DICER1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010817209.3	XP_010815511.1	endoribonuclease Dicer isoform X1
XM_010817208.2	XP_010815510.1	endoribonuclease Dicer isoform X1
XM_010817212.2	XP_010815514.1	endoribonuclease Dicer isoform X1
XM_010817208.3	XP_010815510.1	endoribonuclease Dicer isoform X1
XM_024981616.1	XP_024837384.1	endoribonuclease Dicer isoform X1
XM_015459303.2	XP_015314789.1	endoribonuclease Dicer isoform X1
NM_203359.1	NP_976235.1	endoribonuclease Dicer
XM_015459302.1	XP_015314788.1	endoribonuclease Dicer isoform X1
XM_010817213.2	XP_010815515.1	endoribonuclease Dicer isoform X1
XM_024981613.1	XP_024837381.1	endoribonuclease Dicer isoform X1
XM_010817213.3	XP_010815515.1	endoribonuclease Dicer isoform X1
XM_015459303.1	XP_015314789.1	endoribonuclease Dicer isoform X1
XM_010817209.2	XP_010815511.1	endoribonuclease Dicer isoform X1
XM_024981615.1	XP_024837383.1	endoribonuclease Dicer isoform X1
XM_010817212.3	XP_010815514.1	endoribonuclease Dicer isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa44054	NM_203359.1 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_024981615.1 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_010817213.3 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_010817209.3 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_015459303.2 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_010817212.3 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_024981613.1 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_024981616.1 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_015459302.1 Latest version!	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_010817208.3 Latest version!	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OBa45562	XM_010817208.2	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_010817213.2	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_015459303.1	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_010817209.2	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45562	XM_010817212.2	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

DICER1 ( NM_203359.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_976235.1 Bos taurus dicer 1, ribonuclease III (DICER1), mRNA.

Price & Availability↑

CloneID	OBa44054
Clone ID Related Accession (Same CDS sequence)	NM_203359.1
Accession Version	NM_203359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY386968.1. ##Evidence-Data-START## Transcript exon combination :: AY386968.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACCTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACCGTA TCTCTTTCTG CACTTATCCT GGATGCGCAT 
GAGGGCCGCC TTTCCTACAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGGCTGCC CAGCCGCATG GTGGTGTCCA TATTTGACCC CCCTGTGAAT 
GGGCTTCCTC CTGGTTACGT AGTAAATCAA GACAAAAGTA ACACAGAGAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAAC TGGACGATGA CTTCGAGGAG 
GAGGAAGAGG AGGAGGAGGA CCTGATGTGG AGGGCTCACA AGGAGGATGC GGACGATGAG 
GACGACTTCC TGGAATACGA TCAGGAGCAC ATCAAGTTCA TAGATAACAT GCTGATGGGG 
TCAGGGGCTT TCGTCAAGAA AATCTCTCTT TCTCCTTTTT CTGCCACCGA TTCTGCATAC 
GAGTGGAAAA TGCCCAAAAA GTCCTCCCTT GGTAGTCTGC CATTCTCATC CGATTTTGAG 
GATTTCGACT ACAGCTCCTG GGATGCGATG TGCTATCTGG ATCCGAGCAA AGCCGTTGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTGGACACA 
GGGAAGCAGT CCATCTCTTA CGACCTGCAC ACCGAGCAGT GCATTGCCGA CAAAAGCATA 
GCCGACTGTG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGTGGCGA GAGGGCTGCT 
CAGCTTTTCC TCTGTTCGCT GGGGCTGAAG GTGCTCCCGG TAATTAAAAG GACTGATCGG 
GAAAAGGCCA TGTGCCCGAC CAGGGAGAAT TTCACCAGCC AACAAAAGAA CCTTTCAGGG 
AGCCGTGCGG CTGCCTCTGG AGCCGGCTAC CGAGCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGGTGTTTGA AGATCCCCCC GAGGTGTATG TTTGATCACC CCGAGGCAGA CCGGACACTG 
CGTCACCTGA TCTCGGGTTT TGAAAATTTC GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTACC TTCTACAGGC TTTTACACAC GCCTCCTACC ACTACAATAC GATCACGGAT 
TGTTACCAGC GCTTAGAATT CCTGGGAGAT GCAATTTTGG ACTACCTCAT AACTAAGCAC 
CTTTATGAAG ACCCACGGCA GCACTCCCCA GGGGTCCTGA CTGACCTGCG CTCTGCTCTG 
GTCAACAATA CCATCTTCGC GTCTTTGGCT GTGAAGTATG ACTACCACAA GTACTTTAAG 
GCCGTTTCTC CCGAGCTCTT CCATGTCATC GACGATTTCG TGCAGTTTCA GCTCGAGAAG 
AACGAAATGC AAGGAATGGA TTCTGAGCTT AGGAGATCCG AGGAGGATGA AGAGAAGGAA 
GAGGATATTG AAGTTCCAAA GGCCATGGGG GATATTTTCG AGTCACTCGC GGGCGCCATT 
TACATGGACA GCGGGATGTC GCTGGAGACG GTCTGGCAGG TGTACTATCC CATGATGCGG 
CCGCTGATCG AAAAATTTTC TGCAAATGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAATTACCAA ATTCAGCCCA GCTGAGAGAA CCTACGATGG CAAGGTCAGA 
GTCACCGTGG AAGTGGTCGG GAAGGGGAAG TTCAAAGGCG TTGGCCGAAG TTACAGGATT 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTG CGAAGCCTCA AAGCTAATCA ACCTCAGGTT 
CCCAATAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976235.1
CDS	17..5788
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTVSLSALILDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNGLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPEADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPEITKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	NM_203359.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203359.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AGCACCAGGT TCTCATTATG ACCTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACGGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACCGTA TCTCTTTCTG CACTTATCCT GGATGCGCAT 
GAGGGCCGCC TTTCCTACAT GAGAAGCAAA AAGGTCAGCA ACTGTAATCT GTATCGGCTT 
GGAAAAAAGA AGGGGCTGCC CAGCCGCATG GTGGTGTCCA TATTTGACCC CCCTGTGAAT 
GGGCTTCCTC CTGGTTACGT AGTAAATCAA GACAAAAGTA ACACAGAGAA ATGGGAAAAA 
GATGAAATGA CAAAAGACTG CATGTTGGCT AATGGCAAAC TGGACGATGA CTTCGAGGAG 
GAGGAAGAGG AGGAGGAGGA CCTGATGTGG AGGGCTCACA AGGAGGATGC GGACGATGAG 
GACGACTTCC TGGAATACGA TCAGGAGCAC ATCAAGTTCA TAGATAACAT GCTGATGGGG 
TCAGGGGCTT TCGTCAAGAA AATCTCTCTT TCTCCTTTTT CTGCCACCGA TTCTGCATAC 
GAGTGGAAAA TGCCCAAAAA GTCCTCCCTT GGTAGTCTGC CATTCTCATC CGATTTTGAG 
GATTTCGACT ACAGCTCCTG GGATGCGATG TGCTATCTGG ATCCGAGCAA AGCCGTTGAA 
GAGGATGACT TTGTGGTGGG GTTCTGGAAT CCATCAGAAG AAAACTGTGG TGTGGACACA 
GGGAAGCAGT CCATCTCTTA CGACCTGCAC ACCGAGCAGT GCATTGCCGA CAAAAGCATA 
GCCGACTGTG TGGAAGCCCT GCTGGGCTGC TATTTAACCA GCTGTGGCGA GAGGGCTGCT 
CAGCTTTTCC TCTGTTCGCT GGGGCTGAAG GTGCTCCCGG TAATTAAAAG GACTGATCGG 
GAAAAGGCCA TGTGCCCGAC CAGGGAGAAT TTCACCAGCC AACAAAAGAA CCTTTCAGGG 
AGCCGTGCGG CTGCCTCTGG AGCCGGCTAC CGAGCCTCTG TGTTGAAAGA CTTGGAGTAT 
GGGTGTTTGA AGATCCCCCC GAGGTGTATG TTTGATCACC CCGAGGCAGA CCGGACACTG 
CGTCACCTGA TCTCGGGTTT TGAAAATTTC GAAAAGAAAA TCAACTACAG ATTCAAGAAT 
AAGGCTTACC TTCTACAGGC TTTTACACAC GCCTCCTACC ACTACAATAC GATCACGGAT 
TGTTACCAGC GCTTAGAATT CCTGGGAGAT GCAATTTTGG ACTACCTCAT AACTAAGCAC 
CTTTATGAAG ACCCACGGCA GCACTCCCCA GGGGTCCTGA CTGACCTGCG CTCTGCTCTG 
GTCAACAATA CCATCTTCGC GTCTTTGGCT GTGAAGTATG ACTACCACAA GTACTTTAAG 
GCCGTTTCTC CCGAGCTCTT CCATGTCATC GACGATTTCG TGCAGTTTCA GCTCGAGAAG 
AACGAAATGC AAGGAATGGA TTCTGAGCTT AGGAGATCCG AGGAGGATGA AGAGAAGGAA 
GAGGATATTG AAGTTCCAAA GGCCATGGGG GATATTTTCG AGTCACTCGC GGGCGCCATT 
TACATGGACA GCGGGATGTC GCTGGAGACG GTCTGGCAGG TGTACTATCC CATGATGCGG 
CCGCTGATCG AAAAATTTTC TGCAAATGTG CCCCGTTCCC CTGTGCGAGA ATTGCTTGAA 
ATGGAACCAG AAATTACCAA ATTCAGCCCA GCTGAGAGAA CCTACGATGG CAAGGTCAGA 
GTCACCGTGG AAGTGGTCGG GAAGGGGAAG TTCAAAGGCG TTGGCCGAAG TTACAGGATT 
GCCAAGTCTG CAGCAGCAAG AAGAGCCCTG CGAAGCCTCA AAGCTAATCA ACCTCAGGTT 
CCCAATAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_010817209.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815511.1 Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_010817209.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817209.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815511.1
CDS	337..6105
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_010817209.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817209.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_010817208.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815510.1 Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X1, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_010817208.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815510.1
CDS	478..6246
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_010817208.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817208.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_010817212.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815514.1 Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X4, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_010817212.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815514.1
CDS	139..5907
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_010817212.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817212.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_010817208.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815510.1 Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_010817208.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817208.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815510.1
CDS	367..6135
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_010817208.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817208.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_024981616.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837384.1 Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_024981616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837384.1
CDS	266..6034
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_024981616.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981616.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015459303.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314789.1 Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_015459303.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459303.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314789.1
CDS	199..5967
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015459303.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459303.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015459302.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314788.1 Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X2, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_015459302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314788.1
CDS	506..6274
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015459302.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459302.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_010817213.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815515.1 Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X5, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_010817213.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815515.1
CDS	77..5845
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_010817213.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817213.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_024981613.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837381.1 Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_024981613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837381.1
CDS	309..6077
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_024981613.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981613.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_010817213.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815515.1 Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_010817213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815515.1
CDS	108..5876
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_010817213.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817213.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_015459303.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314789.1 Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X6, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_015459303.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314789.1
CDS	313..6081
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_015459303.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459303.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_010817209.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815511.1 Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X3, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_010817209.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104538.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815511.1
CDS	361..6129
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_010817209.2
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1 ribonuclease III (DICER1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817209.2

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_024981615.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837383.1 Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_024981615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837383.1
CDS	208..5976
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_024981615.1
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981615.1

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DICER1 ( XM_010817212.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815514.1 Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.

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CloneID	OBa45562
Clone ID Related Accession (Same CDS sequence)	XM_010817209.3 , XM_010817208.2 , XM_010817212.2 , XM_010817208.3 , XM_024981616.1 , XM_015459303.2 , XM_015459302.1 , XM_010817213.2 , XM_024981613.1 , XM_010817213.3 , XM_015459303.1 , XM_010817209.2 , XM_024981615.1 , XM_010817212.3
Accession Version	XM_010817212.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	endoribonuclease Dicer isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010817212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815514.1
CDS	370..6138
Translation	MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFNRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKHIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDEEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYVMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGEADTEPVVDDDDVFPPYVLRPEDGPRVTINTAIGHVNRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRESYPRPGQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISIANSSSAENENYCKHSTIVVPENAAHQGANRTSPLENHDQMSVNCRTLFSESPGKLQIEVSTDLTAINGLSYNKSLANGSYDLANRDFCQGNHLNYYKQEIPVQPTTSYPIQNLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPVTAAVPGTTETGEAPPDRTASEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTEKWEKDEMTKDCMLANGKLDDDFEEEEEEEEDLMWRAHKEDADDEDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSATDSAYEWKMPKKSSLGSLPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKAMCPTRENFTSQQKNLSGSRAAASGAGYRASVLKDLEYGCLKIPPRCMFDHPDADRTLRHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS

Target ORF information:

RefSeq Version	XM_010817212.3
Organism	Bos taurus(cattle)
Definition	Bos taurus dicer 1, ribonuclease III (DICER1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010817212.3

ORF Insert Sequence:

ATGAAAAGCC CTGCTTTGCA ACCCCTCAGC ATGGCAGGCC TGCAGCTCAT GACCCCTGCT 
TCCTCACCAA TGGGTCCTTT CTTTGGACTG CCATGGCAAC AAGAAGCAAT TCATGATAAC 
ATTTATACGC CAAGAAAATA TCAGGTTGAA CTGCTTGAAG CAGCTCTGGA TCATAATACC 
ATAGTCTGTT TAAACACTGG CTCAGGGAAG ACGTTTATTG CAGTACTACT CACTAAAGAG 
CTGTCTTATC AGATCAGGGG AGACTTCAAC AGAAATGGCA AAAGGACGGT GTTCTTGGTC 
AACTCTGCAA ACCAGGTTGC CCAACAAGTG TCAGCTGTCA GAACTCACTC AGATCTCAAG 
GTCGGGGAAT ACTCAAACCT AGAAGTAAGT GCATCTTGGA CAAAAGAGAA ATGGAACCAA 
GAGTTTACTA AACATCAGGT TCTCGTTATG ACTTGCTATG TCGCCTTGAA TGTTTTGAAA 
AATGGTTACT TATCACTGTC AGACATTAAC CTTTTGGTGT TTGATGAGTG TCATCTTGCA 
ATCCTAGACC ACCCCTACCG AGAAATTATG AAGCTTTGTG AAAATTGTCC ATCATGTCCT 
CGTATTTTGG GACTAACTGC TTCCATTTTA AATGGGAAAT GTGATCCAGA GGAATTGGAA 
GAGAAGATTC AGAAACTGGA GAAAATTCTT AAGAGTAATG CTGAAACTGC AACTGACTTG 
GTGGTCTTAG ACAGATATAC TTCTCAGCCA TGTGAGATTG TGGTAGACTG TGGACCATTT 
ACTGACAGAA GTGGGCTTTA TGAAAGACTG CTGATGGAGT TAGAAGAAGC ACTTAATTTT 
ATCAATGACT GTAACATATC TGTACATTCA AAAGAAAGAG ATTCTACTTT AATTTCTAAA 
CAGATACTCT CAGACTGCCG TGCGGTCCTG GTTGTCCTGG GACCCTGGTG TGCCGATAAA 
GTAGCTGGAA TGATGGTCAG AGAGCTGCAG AAACACATCA AACATGAGCA GGAGGAGCTG 
CACCGGAAGT TTCTGTTGTT CACAGACACT TTCCTACGGA AAATCCACGC CCTGTGTGAA 
GAGCACTTCT CCCCTGCCTC GCTTGACCTG AAGTTTGTCA CTCCTAAAGT AATAAAGCTG 
CTCGAGATCT TACGCAAATA CAAACCGTAT GAGCGGCAGC AGTTTGAAAG CGTGGAGTGG 
TATAATAATA GGAACCAGGA TAATTACGTG TCCTGGAGCG ATTCTGAGGA TGACGAGGAA 
GATGAAGAGA TTGAAGAGAA AGAAAAGCCA GAGACAAATT TTCCTTCTCC GTTTACCAAC 
ATTTTGTGTG GAATTATTTT TGTGGAAAGA AGATACACAG CCGTGGTCTT AAACAGATTG 
ATAAAGGAAG CTGGCAAACA AGATCCAGAG CTGGCTTACA TCAGCAGCAA TTTTATAACT 
GGACATGGCA TCGGAAAGAA TCAGCCTCGG AACAAACAGA TGGAAGCGGA ATTCAGAAAA 
CAGGAAGAGG TACTTAGGAA ATTTCGAGCA CATGAAACCA ACCTGCTTAT TGCAACAAGT 
ATTGTGGAGG AGGGTGTTGA CATACCGAAA TGCAACTTGG TGGTTCGTTT CGATCTGCCC 
ACAGAGTATC GATCCTACGT TCAGTCCAAG GGAAGAGCGA GGGCACCCAT CTCTAATTAT 
GTAATGTTAG CAGACACAGA TAAAATAAAG AGTTTTGAAG AAGACCTTAA AACATACAAA 
GCTATTGAAA AGATCTTGCG AAACAAATGT TCCAAGTCGG TTGATACCGG GGAGGCCGAC 
ACGGAGCCCG TGGTGGATGA CGACGATGTT TTCCCACCGT ATGTGCTGAG GCCTGAGGAC 
GGTCCCCGCG TCACGATCAA CACGGCCATT GGGCACGTCA ACAGATACTG TGCTAGATTA 
CCAAGTGATC CGTTTACTCA TCTGGCTCCT AAATGTAGAA CCCGAGAGTT GCCTGATGGT 
ACATTTTATT CAACTCTTTA TCTGCCAATT AACTCACCTC TTCGAGCCTC CATTGTTGGT 
CCCCCAATGA GCTGCATTCG GTTGGCTGAA AGAGTCGTGG CTCTCATTTG CTGTGAAAAA 
CTGCACAAAA TTGGCGAACT GGATGACCAT TTGATGCCAG TTGGGAAGGA GACTGTTAAA 
TATGAGGAAG AGCTGGATTT ACACGATGAA GAAGAGACCA GTGTTCCAGG AAGACCAGGT 
TCCACAAAAC GAAGACAGTG CTACCCAAAA GCAATTCCAG AATGTTTGAG GGAGAGCTAC 
CCCAGGCCTG GTCAGCCCTG TTACCTGTAC GTGATCGGAA TGGTCCTGAC GACACCTTTA 
CCTGATGAGC TCAACTTCAG AAGGCGGAAG CTCTACCCCC CTGAGGACAC CACAAGATGC 
TTTGGAATAC TGACAGCCAA GCCCATACCT CAGATCCCAC ACTTTCCTGT GTACACGCGC 
TCTGGAGAGG TGACCATATC TATTGAGTTG AAGAAGTCCG GCTTCACGTT ATCTCTGCAA 
ATGCTTGAGT TGATCACAAG GCTTCACCAG TATATATTCT CACATATTCT TCGGCTTGAA 
AAACCTGCAC TAGAATTTAA ACCCACAGAC GCGGACTCAG CCTACTGCGT TCTACCTCTT 
AATGTTGTTA ATGACTCCAG CACTTTGGAC ATTGACTTTA AATTCATGGA AGATATCGAG 
AAATCTGAAG CTCGAATAGG CATTCCCAGC ACAAAGTATT CAAAAGAAAC TCCTTTTGTT 
TTCAAATTAG AAGATTACCA AGATGCAGTT ATCATTCCAA GATACCGCAA TTTTGATCAG 
CCTCATCGAT TTTATGTCGC TGACGTGTAC ACTGATCTTA CCCCCCTGAG TAAATTTCCT 
TCCCCTGAGT ATGAAACGTT TGCGGAGTAT TATAAAACAA AGTATAACCT TGACCTGACC 
AATCTCAACC AGCCGCTGCT GGACGTGGAC CACACGTCTT CACGACTCAA TCTTTTGACA 
CCTCGTCATT TGAATCAGAA GGGGAAAGCT CTCCCTCTGA GCAGTGCTGA GAAGAGGAAA 
GCCAAGTGGG AGAGCCTGCA GAATAAGCAG ATACTGGTTC CAGAACTCTG TGCTATACAT 
CCAATTCCAG CATCACTGTG GAGAAAAGCC GTTTGTCTCC CGAGCATACT TTACCGCCTT 
CACTGCCTTC TGACTGCAGA GGAGCTCAGA GCCCAGACGG CCAGCGACGC TGGTGTGGGA 
GTCAGGTCAC TTCCTGTGGA TTTTAGATAC CCTAACTTAG ACTTCGGGTG GAAAAAATCT 
ATCGACAGCA AATCTTTCAT CTCAATTGCT AACTCCTCTT CAGCTGAAAA TGAGAATTAC 
TGTAAGCACA GCACAATTGT CGTCCCTGAA AATGCTGCAC ATCAAGGTGC TAATAGAACC 
TCCCCTCTAG AAAATCATGA CCAAATGTCT GTGAACTGCA GAACGTTGTT CAGCGAGTCA 
CCTGGTAAGC TCCAAATTGA AGTTTCAACA GATCTGACAG CAATTAATGG TCTTTCTTAC 
AATAAAAGTC TTGCCAATGG CAGTTACGAT TTAGCTAACA GAGACTTTTG CCAAGGAAAC 
CATCTGAATT ACTACAAGCA GGAAATACCT GTACAACCAA CTACCTCATA TCCCATTCAG 
AATTTATACA ATTACGAGAA CCAGCCCAAG CCCAGCGATG AATGTACTCT ACTGAGTAAT 
AAATACCTTG ATGGAAATGC TAACACATCT ACCTCAGATG GAAGTCCCGT GACGGCTGCC 
GTGCCTGGTA CTACAGAGAC TGGGGAGGCG CCCCCGGACA GGACAGCTTC TGAGCAGAGC 
CCTTCTCCTG GGTACTCCTC GAGGACGCTT GGCCCCAACC CTGGGCTCAT TCTTCAGGCT 
TTGACCCTTT CGAACGCTAG TGATGGATTT AACCTGGAGC GGCTCGAGAT GCTTGGCGAC 
TCCTTCCTCA AGCATGCCAT CACCACGTAT CTCTTCTGCA CTTATCCTGA TGCGCATGAG 
GGCCGCCTTT CCTACATGAG AAGCAAAAAG GTCAGCAACT GTAATCTGTA TCGGCTTGGA 
AAAAAGAAGG GGCTGCCCAG CCGCATGGTG GTGTCCATAT TTGACCCCCC TGTGAATTGG 
CTTCCTCCTG GTTACGTAGT AAATCAAGAC AAAAGTAACA CAGAGAAATG GGAAAAAGAT 
GAAATGACAA AAGACTGCAT GTTGGCTAAT GGCAAACTGG ACGATGACTT CGAGGAGGAG 
GAAGAGGAGG AGGAGGACCT GATGTGGAGG GCTCACAAGG AGGATGCGGA CGATGAGGAC 
GACTTCCTGG AATACGATCA GGAGCACATC AAGTTCATAG ATAACATGCT GATGGGGTCA 
GGGGCTTTCG TCAAGAAAAT CTCTCTTTCT CCTTTTTCTG CCACCGATTC TGCATACGAG 
TGGAAAATGC CCAAAAAGTC CTCCCTTGGT AGTCTGCCAT TCTCATCCGA TTTTGAGGAT 
TTCGACTACA GCTCCTGGGA TGCGATGTGC TATCTGGATC CGAGCAAAGC CGTTGAAGAG 
GATGACTTTG TGGTGGGGTT CTGGAATCCA TCAGAAGAAA ACTGTGGTGT GGACACAGGG 
AAGCAGTCCA TCTCTTACGA CCTGCACACC GAGCAGTGCA TTGCCGACAA AAGCATAGCC 
GACTGTGTGG AAGCCCTGCT GGGCTGCTAT TTAACCAGCT GTGGCGAGAG GGCTGCTCAG 
CTTTTCCTCT GTTCGCTGGG GCTGAAGGTG CTCCCGGTAA TTAAAAGGAC TGATCGGGAA 
AAGGCCATGT GCCCGACCAG GGAGAATTTC ACCAGCCAAC AAAAGAACCT TTCAGGGAGC 
CGTGCGGCCG CCTCTGGAGC CGGCTACCGA GCCTCTGTGT TGAAAGACTT GGAGTATGGG 
TGTTTGAAGA TCCCCCCGAG GTGTATGTTT GATCACCCCG ACGCAGACCG GACACTGCGT 
CACCTGATCT CGGGTTTTGA AAATTTCGAA AAGAAAATCA ACTACAGATT CAAGAATAAG 
GCTTACCTTC TACAGGCTTT TACACACGCC TCCTACCACT ACAATACGAT CACGGATTGT 
TACCAGCGCT TAGAATTCCT GGGAGATGCA ATTTTGGACT ACCTCATAAC TAAGCACCTT 
TATGAAGACC CACGGCAGCA CTCCCCAGGG GTCCTGACTG ACCTGCGCTC TGCTCTGGTC 
AACAATACCA TCTTCGCGTC TTTGGCTGTG AAGTATGACT ACCACAAGTA CTTTAAGGCC 
GTTTCTCCCG AGCTCTTCCA TGTCATCGAC GATTTCGTGC AGTTTCAGCT CGAGAAGAAC 
GAAATGCAAG GAATGGATTC TGAGCTTAGG AGATCCGAGG AGGATGAAGA GAAGGAAGAG 
GATATTGAAG TTCCAAAGGC CATGGGGGAT ATTTTCGAGT CACTCGCGGG CGCCATTTAC 
ATGGACAGCG GGATGTCGCT GGAGACGGTC TGGCAGGTGT ACTATCCCAT GATGCGGCCG 
CTGATCGAAA AATTTTCTGC AAATGTGCCC CGTTCCCCTG TGCGAGAATT GCTTGAAATG 
GAACCAGAAA CTGCCAAATT CAGCCCAGCT GAGAGAACCT ACGATGGCAA GGTCAGAGTC 
ACCGTGGAAG TGGTCGGGAA GGGGAAGTTC AAAGGCGTTG GCCGAAGTTA CAGGATTGCC 
AAGTCTGCAG CAGCAAGAAG AGCCCTGCGA AGCCTCAAAG CTAATCAACC TCAGGTTCCC 
AATAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DICER1
Entrez Gene ID	337871
Full Name	dicer 1, ribonuclease III
Synonyms	Dicer
General protein information	Preferred Names dicer 1, ribonuclease III Names endoribonuclease Dicer Dicer1, Dcr-1 homolog dicer 1, ribonuclease type III ribonuclease III
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DICER1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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