NDST2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDST2
Entrez Gene ID	286838
Full Name	N-deacetylase and N-sulfotransferase 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010820534.3	XP_010818836.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X2
XM_024986575.1	XP_024842343.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X3
XM_010820534.2	XP_010818836.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X2
XM_010820535.2	XP_010818837.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X3
XM_005226331.4	XP_005226388.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X1
XM_005226331.3	XP_005226388.1	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X1
NM_174777.3	NP_777202.2	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NDST2	XP_005619103.1
Caenorhabditis elegans (roundworm)	hst-1	NP_501491.4
Homo sapiens (human)	NDST2	NP_003626.1
Pan troglodytes (chimpanzee)	NDST2	XP_507851.4
Mus musculus (house mouse)	Ndst2	NP_034941.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006328	XP_316352.3
Bos taurus (cattle)	NDST2	NP_777202.2
Danio rerio (zebrafish)	ndst2a	XP_001924050.1
Drosophila melanogaster (fruit fly)	sfl	NP_523946.1
Xenopus tropicalis (tropical clawed frog)	ndst2	NP_001136290.1
Gallus gallus (chicken)	NDST2	XP_421613.2
	NDST2	XP_001104406.2
Rattus norvegicus (Norway rat)	Ndst2	NP_001099210.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray.
Ishiwata H, Katsuma S, Kizaki K, Patel OV, Nakano H, Takahashi T, Imai K, Hirasawa A, Shiojima S, Ikawa H, Suzuki Y, Tsujimoto G, Izaike Y, Todoroki J, Hashizume K
Molecular reproduction and development65(1)9-18(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDST2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDST2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43257	NM_174777.3 Latest version!	Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa34176	XM_005226331.4 Latest version!	Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa34178	XM_024986575.1 Latest version!	Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa34178	XM_010820535.2 Latest version!	Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa45521	XM_010820534.3 Latest version!	Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OBa45521	XM_010820534.2	Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa34176	XM_005226331.3	Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDST2 ( NM_174777.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777202.2 Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), mRNA.

Price & Availability↑

CloneID	OBa43257
Clone ID Related Accession (Same CDS sequence)	NM_174777.3
Accession Version	NM_174777.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC134688.1. On Oct 12, 2007 this sequence version replaced NM_174777.2. ##Evidence-Data-START## Transcript exon combination :: BC134688.1, AF064825.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTTGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAAAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGCATCAGCG AGCACATGGA 
GACCCAGTTG CTCTGAACTA TACCTTCTAC CAGGTGATTT CAGCCTCCTC CCAGGCCCCT 
CCAGCACTTC GCTCCCTACA AAACCGTTGT CTTGTCCCCG GCTACTATTC CACTCACTTA 
CAACGCTGGC TGACTTACTT CCCCTCTGGA CAGTTGCTGA TTGTGGATGG GCAAGAGCTG 
CGTACCAACC CAGCTGCCTC AATGGAGAGC ATCCAGAAGT TCCTGGGTAT CACACCCTTT 
CTGAACTACA CACGGACCCT CAGGTTCGAT GAAGATAAGG GATTCTGGTG CCAGGGACTT 
GAAGGTGGCA AGACTCGTTG TCTAGGAAAG AGCAAAGGTC GGAAGTACCC AGATATGGAC 
GCTGAGTCCC GCCTTTTCCT TACGGATTTT TTCCGGAACC ACAATTTGGA GCTGTCAAAG 
CTGCTGAGCC GGCTTGGACA GCCAGTGCCC TCATGGCTTC GAGAAGAACT GCAGCATTCC 
AGTTCGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777202.2
CDS	578..3229
Translation	MLKLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGAAGGPGPVRPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDHTRGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPTAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRLAEPPVPGPVPRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLVFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRREFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLARKYFDLFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQAPPALRSLQNRCLVPGYYSTHLQRWLTYFPSGQLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDEDKGFWCQGLEGGKTRCLGKSKGRKYPDMDAESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSSG

Target ORF information:

RefSeq Version	NM_174777.3
Organism	Bos taurus(cattle)
Definition	Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174777.3

ORF Insert Sequence:

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTTGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAAAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGCATCAGCG AGCACATGGA 
GACCCAGTTG CTCTGAACTA TACCTTCTAC CAGGTGATTT CAGCCTCCTC CCAGGCCCCT 
CCAGCACTTC GCTCCCTACA AAACCGTTGT CTTGTCCCCG GCTACTATTC CACTCACTTA 
CAACGCTGGC TGACTTACTT CCCCTCTGGA CAGTTGCTGA TTGTGGATGG GCAAGAGCTG 
CGTACCAACC CAGCTGCCTC AATGGAGAGC ATCCAGAAGT TCCTGGGTAT CACACCCTTT 
CTGAACTACA CACGGACCCT CAGGTTCGAT GAAGATAAGG GATTCTGGTG CCAGGGACTT 
GAAGGTGGCA AGACTCGTTG TCTAGGAAAG AGCAAAGGTC GGAAGTACCC AGATATGGAC 
GCTGAGTCCC GCCTTTTCCT TACGGATTTT TTCCGGAACC ACAATTTGGA GCTGTCAAAG 
CTGCTGAGCC GGCTTGGACA GCCAGTGCCC TCATGGCTTC GAGAAGAACT GCAGCATTCC 
AGTTCGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST2 ( XM_005226331.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226388.1 Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa34176
Clone ID Related Accession (Same CDS sequence)	XM_005226331.4 , XM_005226331.3
Accession Version	XM_005226331.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226331.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAGAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGCATCAGCG AGCACATGGA 
GACCCAGTTG CTCTGAACTA TACCTTCTAC CAGGTGATTT CAGCCTCCTC CCAGGCCCCT 
CCAGCACTTC GCTCCCTACA AAACCGTTGT CTTGTCCCCG GCTACTATTC CACTCACTTA 
CAACGCTGGC TGACTTACTT CCCCTCTGGA CAGTTGCTGA TTGTGGATGG GCAAGAGCTG 
CGTACCAACC CAGCTGCCTC AATGGAGAGC ATCCAGAAGT TCCTGGGTAT CACACCCTTT 
CTGAACTACA CACGGACCCT CAGGTTCGAT GAAGATAAGG GATTCTGGTG CCAGGGACTT 
GAAGGTGGCA AGACTCGTTG TCTAGGAAAG AGCAAAGGTC GGAAGTACCC AGATATGGAC 
GCTGAGTCCC GCCTTTTCCT TACGGATTTT TTCCGGAACC ACAATTTGGA GCTGTCAAAG 
CTGCTGAGCC GGCTTGGACA GCCAGTGCCC TCATGGCTTC GAGAAGAACT GCAGCATTCC 
AGTTCGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226388.1
CDS	491..3142
Translation	MLKLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGAAGGPGPVRPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDHTRGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPTAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRLAEPPVPGPVPRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLVFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRREFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLARKYFDLFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQAPPALRSLQNRCLVPGYYSTHLQRWLTYFPSGQLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDEDKGFWCQGLEGGKTRCLGKSKGRKYPDMDAESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSSG

Target ORF information:

RefSeq Version	XM_005226331.4
Organism	Bos taurus(cattle)
Definition	Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226331.4

ORF Insert Sequence:

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAGAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGCATCAGCG AGCACATGGA 
GACCCAGTTG CTCTGAACTA TACCTTCTAC CAGGTGATTT CAGCCTCCTC CCAGGCCCCT 
CCAGCACTTC GCTCCCTACA AAACCGTTGT CTTGTCCCCG GCTACTATTC CACTCACTTA 
CAACGCTGGC TGACTTACTT CCCCTCTGGA CAGTTGCTGA TTGTGGATGG GCAAGAGCTG 
CGTACCAACC CAGCTGCCTC AATGGAGAGC ATCCAGAAGT TCCTGGGTAT CACACCCTTT 
CTGAACTACA CACGGACCCT CAGGTTCGAT GAAGATAAGG GATTCTGGTG CCAGGGACTT 
GAAGGTGGCA AGACTCGTTG TCTAGGAAAG AGCAAAGGTC GGAAGTACCC AGATATGGAC 
GCTGAGTCCC GCCTTTTCCT TACGGATTTT TTCCGGAACC ACAATTTGGA GCTGTCAAAG 
CTGCTGAGCC GGCTTGGACA GCCAGTGCCC TCATGGCTTC GAGAAGAACT GCAGCATTCC 
AGTTCGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST2 ( XM_005226331.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226388.1 Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X1, mRNA.

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CloneID	OBa34176
Clone ID Related Accession (Same CDS sequence)	XM_005226331.4 , XM_005226331.3
Accession Version	XM_005226331.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAGAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGCATCAGCG AGCACATGGA 
GACCCAGTTG CTCTGAACTA TACCTTCTAC CAGGTGATTT CAGCCTCCTC CCAGGCCCCT 
CCAGCACTTC GCTCCCTACA AAACCGTTGT CTTGTCCCCG GCTACTATTC CACTCACTTA 
CAACGCTGGC TGACTTACTT CCCCTCTGGA CAGTTGCTGA TTGTGGATGG GCAAGAGCTG 
CGTACCAACC CAGCTGCCTC AATGGAGAGC ATCCAGAAGT TCCTGGGTAT CACACCCTTT 
CTGAACTACA CACGGACCCT CAGGTTCGAT GAAGATAAGG GATTCTGGTG CCAGGGACTT 
GAAGGTGGCA AGACTCGTTG TCTAGGAAAG AGCAAAGGTC GGAAGTACCC AGATATGGAC 
GCTGAGTCCC GCCTTTTCCT TACGGATTTT TTCCGGAACC ACAATTTGGA GCTGTCAAAG 
CTGCTGAGCC GGCTTGGACA GCCAGTGCCC TCATGGCTTC GAGAAGAACT GCAGCATTCC 
AGTTCGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226388.1
CDS	541..3192
Translation	MLKLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGAAGGPGPVRPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDHTRGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPTAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRLAEPPVPGPVPRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLVFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRREFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLARKYFDLFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQAPPALRSLQNRCLVPGYYSTHLQRWLTYFPSGQLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDEDKGFWCQGLEGGKTRCLGKSKGRKYPDMDAESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSSG

Target ORF information:

RefSeq Version	XM_005226331.3
Organism	Bos taurus(cattle)
Definition	Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226331.3

ORF Insert Sequence:

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAGAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGCATCAGCG AGCACATGGA 
GACCCAGTTG CTCTGAACTA TACCTTCTAC CAGGTGATTT CAGCCTCCTC CCAGGCCCCT 
CCAGCACTTC GCTCCCTACA AAACCGTTGT CTTGTCCCCG GCTACTATTC CACTCACTTA 
CAACGCTGGC TGACTTACTT CCCCTCTGGA CAGTTGCTGA TTGTGGATGG GCAAGAGCTG 
CGTACCAACC CAGCTGCCTC AATGGAGAGC ATCCAGAAGT TCCTGGGTAT CACACCCTTT 
CTGAACTACA CACGGACCCT CAGGTTCGAT GAAGATAAGG GATTCTGGTG CCAGGGACTT 
GAAGGTGGCA AGACTCGTTG TCTAGGAAAG AGCAAAGGTC GGAAGTACCC AGATATGGAC 
GCTGAGTCCC GCCTTTTCCT TACGGATTTT TTCCGGAACC ACAATTTGGA GCTGTCAAAG 
CTGCTGAGCC GGCTTGGACA GCCAGTGCCC TCATGGCTTC GAGAAGAACT GCAGCATTCC 
AGTTCGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST2 ( XM_024986575.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842343.1 Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa34178
Clone ID Related Accession (Same CDS sequence)	XM_024986575.1 , XM_010820535.2
Accession Version	XM_024986575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGT GCTGCCGCGG 
CAGACATGTG GTCTCTTCAC TCACACAATC TTCTATAATG AGTATCCAGG AGGCTCTCGT 
GAACTCGACC GGAGCATCCG AGGTGGAGAA CTCTTTCTGA CAGTGCTGCT TAATCCGATC 
AGCATCTTTA TGACTCATCT GTCTAATTAT GGAAATGATC GGCTGGGCCT GTACACCTTT 
GAGAGCCTGG TGCGCTTTCT CCAGTGCTGG ACGAGGCTGC GCCTACAGAC CCTTCCTCCT 
GTCCCTCTTG CACGAAAGTA CTTTGACCTC TTCCCTCAAG AGCGAAGCCC CCTTTGGCAG 
AATCCCTGTG ACGACAAGAG GCACAAAGAT ATCTGGTCCA AGGAGAAAAC CTGTGATCGG 
CTCCCCAAGT TCCTCATTGT GGGACCCCAG AAGACAGGGA CCACAGCTAT TCACTTCTTC 
CTGAGCCTGC ATCCAGCTGT GACTAGCAGT TTTCCTAGCC CCAGCACCTT TGAGGAGATT 
CAGTTCTTCA ACGGCCCTAA TTACCACAAG GGCATTGACT GGTACATGGA TTTCTTCCCT 
GTCCCTTCCA ATGCCAGCAC TGATTTCCTA TTTGAAAAAA GTGCCACGTA CTTTGACTCA 
GAGGTTGTAC CACGGCGGGG AGCTGCCCTC CTGCCACGAG CCAAGATCAT CACTGTGCTC 
ACCAACCCTG CTGACAGGGC ATACTCCTGG TACCAGCATC AGCGAGCACA TGGAGACCCA 
GTTGCTCTGA ACTATACCTT CTACCAGGTG ATTTCAGCCT CCTCCCAGGC CCCTCCAGCA 
CTTCGCTCCC TACAAAACCG TTGTCTTGTC CCCGGCTACT ATTCCACTCA CTTACAACGC 
TGGCTGACTT ACTTCCCCTC TGGACAGTTG CTGATTGTGG ATGGGCAAGA GCTGCGTACC 
AACCCAGCTG CCTCAATGGA GAGCATCCAG AAGTTCCTGG GTATCACACC CTTTCTGAAC 
TACACACGGA CCCTCAGGTT CGATGAAGAT AAGGGATTCT GGTGCCAGGG ACTTGAAGGT 
GGCAAGACTC GTTGTCTAGG AAAGAGCAAA GGTCGGAAGT ACCCAGATAT GGACGCTGAG 
TCCCGCCTTT TCCTTACGGA TTTTTTCCGG AACCACAATT TGGAGCTGTC AAAGCTGCTG 
AGCCGGCTTG GACAGCCAGT GCCCTCATGG CTTCGAGAAG AACTGCAGCA TTCCAGTTCG 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842343.1
CDS	2134..4599
Translation	MLKLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGAAGGPGPVRPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDHTRGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPTAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRLAEPPVPGPVPRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLVFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRREFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALVLPRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLARKYFDLFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQAPPALRSLQNRCLVPGYYSTHLQRWLTYFPSGQLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDEDKGFWCQGLEGGKTRCLGKSKGRKYPDMDAESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSSG

Target ORF information:

RefSeq Version	XM_024986575.1
Organism	Bos taurus(cattle)
Definition	Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986575.1

ORF Insert Sequence:

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGT GCTGCCGCGG 
CAGACATGTG GTCTCTTCAC TCACACAATC TTCTATAATG AGTATCCAGG AGGCTCTCGT 
GAACTCGACC GGAGCATCCG AGGTGGAGAA CTCTTTCTGA CAGTGCTGCT TAATCCGATC 
AGCATCTTTA TGACTCATCT GTCTAATTAT GGAAATGATC GGCTGGGCCT GTACACCTTT 
GAGAGCCTGG TGCGCTTTCT CCAGTGCTGG ACGAGGCTGC GCCTACAGAC CCTTCCTCCT 
GTCCCTCTTG CACGAAAGTA CTTTGACCTC TTCCCTCAAG AGCGAAGCCC CCTTTGGCAG 
AATCCCTGTG ACGACAAGAG GCACAAAGAT ATCTGGTCCA AGGAGAAAAC CTGTGATCGG 
CTCCCCAAGT TCCTCATTGT GGGACCCCAG AAGACAGGGA CCACAGCTAT TCACTTCTTC 
CTGAGCCTGC ATCCAGCTGT GACTAGCAGT TTTCCTAGCC CCAGCACCTT TGAGGAGATT 
CAGTTCTTCA ACGGCCCTAA TTACCACAAG GGCATTGACT GGTACATGGA TTTCTTCCCT 
GTCCCTTCCA ATGCCAGCAC TGATTTCCTA TTTGAAAAAA GTGCCACGTA CTTTGACTCA 
GAGGTTGTAC CACGGCGGGG AGCTGCCCTC CTGCCACGAG CCAAGATCAT CACTGTGCTC 
ACCAACCCTG CTGACAGGGC ATACTCCTGG TACCAGCATC AGCGAGCACA TGGAGACCCA 
GTTGCTCTGA ACTATACCTT CTACCAGGTG ATTTCAGCCT CCTCCCAGGC CCCTCCAGCA 
CTTCGCTCCC TACAAAACCG TTGTCTTGTC CCCGGCTACT ATTCCACTCA CTTACAACGC 
TGGCTGACTT ACTTCCCCTC TGGACAGTTG CTGATTGTGG ATGGGCAAGA GCTGCGTACC 
AACCCAGCTG CCTCAATGGA GAGCATCCAG AAGTTCCTGG GTATCACACC CTTTCTGAAC 
TACACACGGA CCCTCAGGTT CGATGAAGAT AAGGGATTCT GGTGCCAGGG ACTTGAAGGT 
GGCAAGACTC GTTGTCTAGG AAAGAGCAAA GGTCGGAAGT ACCCAGATAT GGACGCTGAG 
TCCCGCCTTT TCCTTACGGA TTTTTTCCGG AACCACAATT TGGAGCTGTC AAAGCTGCTG 
AGCCGGCTTG GACAGCCAGT GCCCTCATGG CTTCGAGAAG AACTGCAGCA TTCCAGTTCG 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST2 ( XM_010820535.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818837.1 Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa34178
Clone ID Related Accession (Same CDS sequence)	XM_024986575.1 , XM_010820535.2
Accession Version	XM_010820535.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820535.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGT GCTGCCGCGG 
CAGACATGTG GTCTCTTCAC TCACACAATC TTCTATAATG AGTATCCAGG AGGCTCTCGT 
GAACTCGACC GGAGCATCCG AGGTGGAGAA CTCTTTCTGA CAGTGCTGCT TAATCCGATC 
AGCATCTTTA TGACTCATCT GTCTAATTAT GGAAATGATC GGCTGGGCCT GTACACCTTT 
GAGAGCCTGG TGCGCTTTCT CCAGTGCTGG ACGAGGCTGC GCCTACAGAC CCTTCCTCCT 
GTCCCTCTTG CACGAAAGTA CTTTGACCTC TTCCCTCAAG AGCGAAGCCC CCTTTGGCAG 
AATCCCTGTG ACGACAAGAG GCACAAAGAT ATCTGGTCCA AGGAGAAAAC CTGTGATCGG 
CTCCCCAAGT TCCTCATTGT GGGACCCCAG AAGACAGGGA CCACAGCTAT TCACTTCTTC 
CTGAGCCTGC ATCCAGCTGT GACTAGCAGT TTTCCTAGCC CCAGCACCTT TGAGGAGATT 
CAGTTCTTCA ACGGCCCTAA TTACCACAAG GGCATTGACT GGTACATGGA TTTCTTCCCT 
GTCCCTTCCA ATGCCAGCAC TGATTTCCTA TTTGAAAAAA GTGCCACGTA CTTTGACTCA 
GAGGTTGTAC CACGGCGGGG AGCTGCCCTC CTGCCACGAG CCAAGATCAT CACTGTGCTC 
ACCAACCCTG CTGACAGGGC ATACTCCTGG TACCAGCATC AGCGAGCACA TGGAGACCCA 
GTTGCTCTGA ACTATACCTT CTACCAGGTG ATTTCAGCCT CCTCCCAGGC CCCTCCAGCA 
CTTCGCTCCC TACAAAACCG TTGTCTTGTC CCCGGCTACT ATTCCACTCA CTTACAACGC 
TGGCTGACTT ACTTCCCCTC TGGACAGTTG CTGATTGTGG ATGGGCAAGA GCTGCGTACC 
AACCCAGCTG CCTCAATGGA GAGCATCCAG AAGTTCCTGG GTATCACACC CTTTCTGAAC 
TACACACGGA CCCTCAGGTT CGATGAAGAT AAGGGATTCT GGTGCCAGGG ACTTGAAGGT 
GGCAAGACTC GTTGTCTAGG AAAGAGCAAA GGTCGGAAGT ACCCAGATAT GGACGCTGAG 
TCCCGCCTTT TCCTTACGGA TTTTTTCCGG AACCACAATT TGGAGCTGTC AAAGCTGCTG 
AGCCGGCTTG GACAGCCAGT GCCCTCATGG CTTCGAGAAG AACTGCAGCA TTCCAGTTCG 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818837.1
CDS	2478..4943
Translation	MLKLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGAAGGPGPVRPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDHTRGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPTAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRLAEPPVPGPVPRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLVFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRREFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALVLPRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLARKYFDLFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQAPPALRSLQNRCLVPGYYSTHLQRWLTYFPSGQLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDEDKGFWCQGLEGGKTRCLGKSKGRKYPDMDAESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSSG

Target ORF information:

RefSeq Version	XM_010820535.2
Organism	Bos taurus(cattle)
Definition	Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820535.2

ORF Insert Sequence:

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGT GCTGCCGCGG 
CAGACATGTG GTCTCTTCAC TCACACAATC TTCTATAATG AGTATCCAGG AGGCTCTCGT 
GAACTCGACC GGAGCATCCG AGGTGGAGAA CTCTTTCTGA CAGTGCTGCT TAATCCGATC 
AGCATCTTTA TGACTCATCT GTCTAATTAT GGAAATGATC GGCTGGGCCT GTACACCTTT 
GAGAGCCTGG TGCGCTTTCT CCAGTGCTGG ACGAGGCTGC GCCTACAGAC CCTTCCTCCT 
GTCCCTCTTG CACGAAAGTA CTTTGACCTC TTCCCTCAAG AGCGAAGCCC CCTTTGGCAG 
AATCCCTGTG ACGACAAGAG GCACAAAGAT ATCTGGTCCA AGGAGAAAAC CTGTGATCGG 
CTCCCCAAGT TCCTCATTGT GGGACCCCAG AAGACAGGGA CCACAGCTAT TCACTTCTTC 
CTGAGCCTGC ATCCAGCTGT GACTAGCAGT TTTCCTAGCC CCAGCACCTT TGAGGAGATT 
CAGTTCTTCA ACGGCCCTAA TTACCACAAG GGCATTGACT GGTACATGGA TTTCTTCCCT 
GTCCCTTCCA ATGCCAGCAC TGATTTCCTA TTTGAAAAAA GTGCCACGTA CTTTGACTCA 
GAGGTTGTAC CACGGCGGGG AGCTGCCCTC CTGCCACGAG CCAAGATCAT CACTGTGCTC 
ACCAACCCTG CTGACAGGGC ATACTCCTGG TACCAGCATC AGCGAGCACA TGGAGACCCA 
GTTGCTCTGA ACTATACCTT CTACCAGGTG ATTTCAGCCT CCTCCCAGGC CCCTCCAGCA 
CTTCGCTCCC TACAAAACCG TTGTCTTGTC CCCGGCTACT ATTCCACTCA CTTACAACGC 
TGGCTGACTT ACTTCCCCTC TGGACAGTTG CTGATTGTGG ATGGGCAAGA GCTGCGTACC 
AACCCAGCTG CCTCAATGGA GAGCATCCAG AAGTTCCTGG GTATCACACC CTTTCTGAAC 
TACACACGGA CCCTCAGGTT CGATGAAGAT AAGGGATTCT GGTGCCAGGG ACTTGAAGGT 
GGCAAGACTC GTTGTCTAGG AAAGAGCAAA GGTCGGAAGT ACCCAGATAT GGACGCTGAG 
TCCCGCCTTT TCCTTACGGA TTTTTTCCGG AACCACAATT TGGAGCTGTC AAAGCTGCTG 
AGCCGGCTTG GACAGCCAGT GCCCTCATGG CTTCGAGAAG AACTGCAGCA TTCCAGTTCG 
GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST2 ( XM_010820534.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818836.1 Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45521
Clone ID Related Accession (Same CDS sequence)	XM_010820534.3 , XM_010820534.2
Accession Version	XM_010820534.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAGAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGGTGATTTC AGCCTCCTCC 
CAGGCCCCTC CAGCACTTCG CTCCCTACAA AACCGTTGTC TTGTCCCCGG CTACTATTCC 
ACTCACTTAC AACGCTGGCT GACTTACTTC CCCTCTGGAC AGTTGCTGAT TGTGGATGGG 
CAAGAGCTGC GTACCAACCC AGCTGCCTCA ATGGAGAGCA TCCAGAAGTT CCTGGGTATC 
ACACCCTTTC TGAACTACAC ACGGACCCTC AGGTTCGATG AAGATAAGGG ATTCTGGTGC 
CAGGGACTTG AAGGTGGCAA GACTCGTTGT CTAGGAAAGA GCAAAGGTCG GAAGTACCCA 
GATATGGACG CTGAGTCCCG CCTTTTCCTT ACGGATTTTT TCCGGAACCA CAATTTGGAG 
CTGTCAAAGC TGCTGAGCCG GCTTGGACAG CCAGTGCCCT CATGGCTTCG AGAAGAACTG 
CAGCATTCCA GTTCGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818836.1
CDS	2138..4738
Translation	MLKLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGAAGGPGPVRPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDHTRGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPTAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRLAEPPVPGPVPRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLVFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRREFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLARKYFDLFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQVISASSQAPPALRSLQNRCLVPGYYSTHLQRWLTYFPSGQLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDEDKGFWCQGLEGGKTRCLGKSKGRKYPDMDAESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSSG

Target ORF information:

RefSeq Version	XM_010820534.3
Organism	Bos taurus(cattle)
Definition	Bos taurus N-deacetylase and N-sulfotransferase 2 (NDST2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820534.3

ORF Insert Sequence:

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAGAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGGTGATTTC AGCCTCCTCC 
CAGGCCCCTC CAGCACTTCG CTCCCTACAA AACCGTTGTC TTGTCCCCGG CTACTATTCC 
ACTCACTTAC AACGCTGGCT GACTTACTTC CCCTCTGGAC AGTTGCTGAT TGTGGATGGG 
CAAGAGCTGC GTACCAACCC AGCTGCCTCA ATGGAGAGCA TCCAGAAGTT CCTGGGTATC 
ACACCCTTTC TGAACTACAC ACGGACCCTC AGGTTCGATG AAGATAAGGG ATTCTGGTGC 
CAGGGACTTG AAGGTGGCAA GACTCGTTGT CTAGGAAAGA GCAAAGGTCG GAAGTACCCA 
GATATGGACG CTGAGTCCCG CCTTTTCCTT ACGGATTTTT TCCGGAACCA CAATTTGGAG 
CTGTCAAAGC TGCTGAGCCG GCTTGGACAG CCAGTGCCCT CATGGCTTCG AGAAGAACTG 
CAGCATTCCA GTTCGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDST2 ( XM_010820534.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818836.1 Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X2, mRNA.

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CloneID	OBa45521
Clone ID Related Accession (Same CDS sequence)	XM_010820534.3 , XM_010820534.2
Accession Version	XM_010820534.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAGAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGGTGATTTC AGCCTCCTCC 
CAGGCCCCTC CAGCACTTCG CTCCCTACAA AACCGTTGTC TTGTCCCCGG CTACTATTCC 
ACTCACTTAC AACGCTGGCT GACTTACTTC CCCTCTGGAC AGTTGCTGAT TGTGGATGGG 
CAAGAGCTGC GTACCAACCC AGCTGCCTCA ATGGAGAGCA TCCAGAAGTT CCTGGGTATC 
ACACCCTTTC TGAACTACAC ACGGACCCTC AGGTTCGATG AAGATAAGGG ATTCTGGTGC 
CAGGGACTTG AAGGTGGCAA GACTCGTTGT CTAGGAAAGA GCAAAGGTCG GAAGTACCCA 
GATATGGACG CTGAGTCCCG CCTTTTCCTT ACGGATTTTT TCCGGAACCA CAATTTGGAG 
CTGTCAAAGC TGCTGAGCCG GCTTGGACAG CCAGTGCCCT CATGGCTTCG AGAAGAACTG 
CAGCATTCCA GTTCGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818836.1
CDS	2479..5079
Translation	MLKLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGAAGGPGPVRPPVPPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDHTRGRYVLVIYENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPTAPLLHLTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRLAEPPVPGPVPRRARLPTVVQDLGLHDGIQRVLFGHGLSFWLHKLVFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPNFTFNLGFSGKFYHTGTEEEDAGDDMLLKHRREFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGIPTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTLPPVPLARKYFDLFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLTNPADRAYSWYQVISASSQAPPALRSLQNRCLVPGYYSTHLQRWLTYFPSGQLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDEDKGFWCQGLEGGKTRCLGKSKGRKYPDMDAESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSSG

Target ORF information:

RefSeq Version	XM_010820534.2
Organism	Bos taurus(cattle)
Definition	Bos taurus N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820534.2

ORF Insert Sequence:

ATGCTCAAGC TGTGGAAGGT GGTACGCCCA GCTCGGCAGC TGGAACTGCA CCGCCTCATA 
CTGCTGCTGA TTGCTTTCAG TCTGGGCTCC ATGGGCTTCT TGGCTTACTA CGTATCCACC 
AGCCCCAAGG CCAAGGAACC GTTGCCCCTC CCCTTGGGAG ACTGCAGCAG TGGCGCAGCG 
GGTGGTCCTG GCCCGGTTCG GCCTCCAGTC CCACCCCGGC CCCCCAGGCC TCCAGAGACA 
GCGCGAACTG AACCCGTGGT CCTTGTGTTT GTGGAGAGTG CATACTCGCA GCTGGGGCAG 
GAGATCGTGG CCATCTTGGA GTCTAGCCGT TTTCGTTACA GCACTGAGCT GGCACCTGGC 
CGAGGGGACA TGCCCACACT GACGGATCAT ACCCGGGGCC GGTATGTCTT GGTCATTTAT 
GAGAACCTGC TCAAGTACGT CAACCTGGAT GCCTGGAGTC GGGAACTGCT AGACCGGTAC 
TGTGTGGAGT ACGGTGTAGG CATCATTGGC TTTTTCCGAG CCCATGAGCA CAGCCTACTG 
AGTGCCCAGC TCAAGGGCTT TCCCCTTTTT CTCCATTCAA ACTTGGGGCT TCGGGACTAC 
CAAGTGAATC CTACAGCTCC ACTCCTACAT CTCACACGTC CCAGCCGCCT GGAGCCTGGG 
CCACTACCCG GTGATGACTG GACCATCTTC CAATCCAACC ACAGCACCTA TGAACCAGTG 
CTTCTCGCCA GCCTTCGGCT GGCTGAGCCC CCTGTGCCAG GACCAGTGCC TCGCCGGGCC 
CGGCTTCCCA CTGTGGTGCA GGACCTGGGG CTTCACGATG GCATCCAGCG GGTGCTGTTT 
GGTCACGGCC TCTCCTTCTG GCTCCACAAA CTAGTTTTCG TCGATGCCGT TGCATACCTC 
ACTGGCAAGC GCCTCTGCTT GGACCTTGAC CGTTACATCT TGGTAGACAT CGATGACATC 
TTTGTGGGCA AGGAAGGTAC CCGCATGAAG GTGGCTGATG TTGAGGCCCT GTTGACCACG 
CAGAACAAAC TCAGGACCTT AGTCCCCAAC TTCACCTTCA ACTTGGGCTT CTCGGGCAAG 
TTCTATCATA CTGGGACAGA GGAGGAGGAT GCTGGGGACG ACATGCTGCT GAAGCACCGC 
CGAGAGTTCT GGTGGTTCCC CCACATGTGG AGCCACATGC AGCCACATCT GTTCCACAAT 
CGCTCCGTGC TGGCTGACCA GATGAGGCTC AACAAACAGT TTGCTCTGGA ACATGGGATT 
CCCACGGATC TGGGGTATGC TGTGGCCCCC CACCACTCCG GCGTGTACCC CATCCACACA 
CAGCTCTATG AAGCCTGGAA ATCTGTGTGG GGCATCCAGG TGACCAGCAC TGAGGAGTAC 
CCCCACCTCC GCCCTGCCCG CTACCGCCGT GGCTTCATTC ACAATGGCAT TATGGTGCTG 
CCGCGGCAGA CATGTGGTCT CTTCACTCAC ACAATCTTCT ATAATGAGTA TCCAGGAGGC 
TCTCGTGAAC TCGACCGGAG CATCCGAGGT GGAGAACTCT TTCTGACAGT GCTGCTTAAT 
CCGATCAGCA TCTTTATGAC TCATCTGTCT AATTATGGAA ATGATCGGCT GGGCCTGTAC 
ACCTTTGAGA GCCTGGTGCG CTTTCTCCAG TGCTGGACGA GGCTGCGCCT ACAGACCCTT 
CCTCCTGTCC CTCTTGCACG AAAGTACTTT GACCTCTTCC CTCAAGAGCG AAGCCCCCTT 
TGGCAGAATC CCTGTGACGA CAAGAGGCAC AAAGATATCT GGTCCAAGGA GAAAACCTGT 
GATCGGCTCC CCAAGTTCCT CATTGTGGGA CCCCAGAAGA CAGGGACCAC AGCTATTCAC 
TTCTTCCTGA GCCTGCATCC AGCTGTGACT AGCAGTTTTC CTAGCCCCAG CACCTTTGAG 
GAGATTCAGT TCTTCAACGG CCCTAATTAC CACAAGGGCA TTGACTGGTA CATGGATTTC 
TTCCCTGTCC CTTCCAATGC CAGCACTGAT TTCCTATTTG AAAAAAGTGC CACGTACTTT 
GACTCAGAGG TTGTACCACG GCGGGGAGCT GCCCTCCTGC CACGAGCCAA GATCATCACT 
GTGCTCACCA ACCCTGCTGA CAGGGCATAC TCCTGGTACC AGGTGATTTC AGCCTCCTCC 
CAGGCCCCTC CAGCACTTCG CTCCCTACAA AACCGTTGTC TTGTCCCCGG CTACTATTCC 
ACTCACTTAC AACGCTGGCT GACTTACTTC CCCTCTGGAC AGTTGCTGAT TGTGGATGGG 
CAAGAGCTGC GTACCAACCC AGCTGCCTCA ATGGAGAGCA TCCAGAAGTT CCTGGGTATC 
ACACCCTTTC TGAACTACAC ACGGACCCTC AGGTTCGATG AAGATAAGGG ATTCTGGTGC 
CAGGGACTTG AAGGTGGCAA GACTCGTTGT CTAGGAAAGA GCAAAGGTCG GAAGTACCCA 
GATATGGACG CTGAGTCCCG CCTTTTCCTT ACGGATTTTT TCCGGAACCA CAATTTGGAG 
CTGTCAAAGC TGCTGAGCCG GCTTGGACAG CCAGTGCCCT CATGGCTTCG AGAAGAACTG 
CAGCATTCCA GTTCGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray.
Molecular reproduction and development65(1)9-18(2003 May)
Ishiwata H,Katsuma S,Kizaki K,Patel OV,Nakano H,Takahashi T,Imai K,Hirasawa A,Shiojima S,Ikawa H,Suzuki Y,Tsujimoto G,Izaike Y,Todoroki J,Hashizume K

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NDST2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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