PCK2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCK2
Entrez Gene ID	282856
Full Name	phosphoenolpyruvate carboxykinase 2, mitochondrial
General protein information	Preferred Names phosphoenolpyruvate carboxykinase 2, mitochondrial Names phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024997291.1	XP_024853059.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform X1
NM_001205594.1	NP_001192523.1	phosphoenolpyruvate carboxykinase [GTP], mitochondrial

Pathways from BioSystems

Homologies

	PCK2	XP_001111487.1
Canis lupus familiaris (dog)	PCK2	XP_537379.2
Mus musculus (house mouse)	Pck2	NP_083270.1
Rattus norvegicus (Norway rat)	Pck2	NP_001101847.2
Homo sapiens (human)	PCK2	NP_004554.2
Drosophila melanogaster (fruit fly)	Pepck	NP_523784.2
Caenorhabditis elegans (roundworm)	pck-1	NP_497134.1
Pan troglodytes (chimpanzee)	PCK2	XP_001165863.1
Bos taurus (cattle)	PCK2	NP_001192523.1
Danio rerio (zebrafish)	pck2	NP_998357.1
Xenopus tropicalis (tropical clawed frog)	pck2	NP_001034816.1
Gallus gallus (chicken)	PCK2	NP_990801.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003350	XP_003436452.1
Caenorhabditis elegans (roundworm)	pck-2	NP_001021587.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cloning and characterization of bovine cytosolic and mitochondrial PEPCK during transition to lactation.
Agca C, Greenfield RB, Hartwell JR, Donkin SS
Physiological genomics11(2)53-63(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transition to lactation does not alter expression of the mitochondrial form of PEPCK.
Title: Cloning and characterization of bovine cytosolic and mitochondrial PEPCK during transition to lactation.

The following PCK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41214	NM_001205594.1 Latest version!	Bos taurus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa45499	XM_024997291.1 Latest version!	Bos taurus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCK2 ( NM_001205594.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192523.1 Bos taurus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.

Price & Availability↑

CloneID	OBa41214
Clone ID Related Accession (Same CDS sequence)	NM_001205594.1
Accession Version	NM_001205594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-24
Organism	Bos taurus(cattle)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000010.1. On Apr 20, 2011 this sequence version replaced XM_583200.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.14280.1, SRR5571260.39058.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCGCTG TGTACCGCCC CGGCCTGCGG CTTAGCTGGC ACAGGCTGAG CCCCTGGGGT 
TGGGCGTCAC GGTGTAGCAT CCAGACGCTG CGAGTCCTCA GCGGAGATCT GGGCCAACTG 
CCTGCTAAGG TTCGAGACTT TGTGGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGGACCGA GGCGGAGAAC ACTGCCACAC TGGCCCTGCT GGAACAGCAG 
GGACTCATCC GAAAACTCCC TAAGTACAAC AATTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAACG GGACACGGTG 
CCACTCCCGG CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGAGTTC 
CAGCAAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCTGG GCCGAACCAT GTACGTGATT 
CCGTTCAGCA TGGGTCCCGT GGGCTCCCCG CTGTCCCGCA TCGGAGTGCA GCTCACGGAC 
TCTGCCTACG TGGTGGCAAG CATGCGGATT ATGACTCGGT TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGCGACTT TGTCAAGTGT CTTCACTCTG TGGGCCAACC CTTGACTGGG 
CAAGGGGAGC CGGTGAGCCA GTGGCCATGC AATCCAGAGA AAACCCTGAT TGGCCACGTG 
CCTGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTTCG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCG 
GAGCACATGC TGATCCTAGG TATCACCAAT CCAGCGGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCCA GTGCTTGTGG CAAGACAAAC CTGGCCATGA TGCGGCCGGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGACATC GCCTGGATGA GGTTTGACAG TGATGGTCGA 
CTCCGGGCCA TCAACCCTGA GAATGGCTTC TTTGGGGTGG CCCCTGGCAC CTCTGCCACC 
ACCAACCCCA ATGCTATGGC CACAATCCAG AGTAACACCA TTTTCACCAA CGTGGCTGAG 
ACCAGCGATG GCGGCGTGTA TTGGGAGGGC ATTGATCAGC CTCTTCCACC TGGCATCACC 
GTGACCTCCT GGCTAGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCGCATCCC 
AACTCTCGCT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TAGACCCCGC CTGGGAGGCC 
CCAGAGGGAG TCCCCATTGA CGCCATCATC TTTGGAGGCC GTAGACCCAA AGGAGTCCCC 
CTGGTATATG AGGCCTTCAA CTGGCGCCAC GGGGTGTTTG TGGGCAGTGC CATGCGCTCC 
GAGGCCACTG CAGCGGCTGA ACACAAAGGG AAGGTCATCA TGCATGACCC ATTTGCCATG 
CGGCCGTTTT TCGGCTACAA CTTTGGGCGC TACCTTGAAC ACTGGCTGAG CATGGAGGGG 
CGCAAGGGGG TCCAGCTGCC CCGCATCTTC CACGTCAACT GGTTCCGGCG CGACGAGGCA 
GGCCGCTTCC TGTGGCCAGG CTTTGGAGAG AATGCCCGGG TGCTAGACTG GATCTGCCGA 
CGGCTCGACG GGGAGGAGAG TGCCCGGGAG ACACCCATCG GACTGGTGCC TAAGGAAGGC 
GCCTTGGATC TCAGCAGCCT GGGAGCCATT GACACCACCC AGTTGTTCTC CCTCCCCAAG 
GACTTTTGGC AACAGGAGGT TCATGACATT CGGAGCTATC TGACAGAACA GGTCAACCAG 
GATCTGCCCA AGGAAGTGCT GGCTGAACTG GAGGCCTTGG AGGGACGTGT GCGCAGAATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192523.1
CDS	115..2037
Translation	MAAVYRPGLRLSWHRLSPWGWASRCSIQTLRVLSGDLGQLPAKVRDFVEHSARLCQPEGIHICDGTEAENTATLALLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLPAGGARGQLGNWMSPAEFQQAVDERFPGCMLGRTMYVIPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITNPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSDGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGITVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIIDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGRYLEHWLSMEGRKGVQLPRIFHVNWFRRDEAGRFLWPGFGENARVLDWICRRLDGEESARETPIGLVPKEGALDLSSLGAIDTTQLFSLPKDFWQQEVHDIRSYLTEQVNQDLPKEVLAELEALEGRVRRM

Target ORF information:

RefSeq Version	NM_001205594.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205594.1

ORF Insert Sequence:

ATGGCCGCTG TGTACCGCCC CGGCCTGCGG CTTAGCTGGC ACAGGCTGAG CCCCTGGGGT 
TGGGCGTCAC GGTGTAGCAT CCAGACGCTG CGAGTCCTCA GCGGAGATCT GGGCCAACTG 
CCTGCTAAGG TTCGAGACTT TGTGGAGCAC AGTGCCCGCC TGTGCCAACC AGAGGGCATC 
CACATCTGTG ATGGGACCGA GGCGGAGAAC ACTGCCACAC TGGCCCTGCT GGAACAGCAG 
GGACTCATCC GAAAACTCCC TAAGTACAAC AATTGCTGGC TGGCCCGCAC AGACCCCAAG 
GATGTGGCAC GAGTAGAGAG CAAGACGGTG ATTGTAACTC CTTCTCAACG GGACACGGTG 
CCACTCCCGG CTGGTGGGGC CCGTGGGCAG CTGGGCAACT GGATGTCCCC AGCTGAGTTC 
CAGCAAGCTG TGGATGAGAG GTTTCCAGGC TGCATGCTGG GCCGAACCAT GTACGTGATT 
CCGTTCAGCA TGGGTCCCGT GGGCTCCCCG CTGTCCCGCA TCGGAGTGCA GCTCACGGAC 
TCTGCCTACG TGGTGGCAAG CATGCGGATT ATGACTCGGT TGGGGACACC TGTGCTTCAG 
GCCCTGGGAG ATGGCGACTT TGTCAAGTGT CTTCACTCTG TGGGCCAACC CTTGACTGGG 
CAAGGGGAGC CGGTGAGCCA GTGGCCATGC AATCCAGAGA AAACCCTGAT TGGCCACGTG 
CCTGACCAGC GGGAGATCAT CTCCTTCGGC AGCGGCTATG GTGGCAACTC CCTGCTGGGC 
AAGAAGTGCT TTGCCCTTCG CATCGCCTCT CGGCTGGCCC GGGATGAGGG CTGGCTGGCG 
GAGCACATGC TGATCCTAGG TATCACCAAT CCAGCGGGGA AGAAGCGCTA TGTGGCAGCC 
GCCTTCCCCA GTGCTTGTGG CAAGACAAAC CTGGCCATGA TGCGGCCGGC ACTGCCAGGC 
TGGAAAGTGG AGTGTGTGGG GGATGACATC GCCTGGATGA GGTTTGACAG TGATGGTCGA 
CTCCGGGCCA TCAACCCTGA GAATGGCTTC TTTGGGGTGG CCCCTGGCAC CTCTGCCACC 
ACCAACCCCA ATGCTATGGC CACAATCCAG AGTAACACCA TTTTCACCAA CGTGGCTGAG 
ACCAGCGATG GCGGCGTGTA TTGGGAGGGC ATTGATCAGC CTCTTCCACC TGGCATCACC 
GTGACCTCCT GGCTAGGCAA ACCCTGGAAA CCTGGTGACA AGGAGCCCTG TGCGCATCCC 
AACTCTCGCT TTTGTGCCCC GGCTCGCCAG TGCCCCATCA TAGACCCCGC CTGGGAGGCC 
CCAGAGGGAG TCCCCATTGA CGCCATCATC TTTGGAGGCC GTAGACCCAA AGGAGTCCCC 
CTGGTATATG AGGCCTTCAA CTGGCGCCAC GGGGTGTTTG TGGGCAGTGC CATGCGCTCC 
GAGGCCACTG CAGCGGCTGA ACACAAAGGG AAGGTCATCA TGCATGACCC ATTTGCCATG 
CGGCCGTTTT TCGGCTACAA CTTTGGGCGC TACCTTGAAC ACTGGCTGAG CATGGAGGGG 
CGCAAGGGGG TCCAGCTGCC CCGCATCTTC CACGTCAACT GGTTCCGGCG CGACGAGGCA 
GGCCGCTTCC TGTGGCCAGG CTTTGGAGAG AATGCCCGGG TGCTAGACTG GATCTGCCGA 
CGGCTCGACG GGGAGGAGAG TGCCCGGGAG ACACCCATCG GACTGGTGCC TAAGGAAGGC 
GCCTTGGATC TCAGCAGCCT GGGAGCCATT GACACCACCC AGTTGTTCTC CCTCCCCAAG 
GACTTTTGGC AACAGGAGGT TCATGACATT CGGAGCTATC TGACAGAACA GGTCAACCAG 
GATCTGCCCA AGGAAGTGCT GGCTGAACTG GAGGCCTTGG AGGGACGTGT GCGCAGAATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCK2 ( XM_024997291.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853059.1 Bos taurus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45499
Clone ID Related Accession (Same CDS sequence)	XM_024997291.1
Accession Version	XM_024997291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphoenolpyruvate carboxykinase [GTP], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACCGA GGCGGAGAAC ACTGCCACAC TGGCCCTGCT GGAACAGCAG GGACTCATCC 
GAAAACTCCC TAAGTACAAC AATTGGCCGA ACCATGTACG TGATTCCGTT CAGCATGGGT 
CCCGTGGGCT CCCCGCTGTC CCGCATCGGA GTGCAGCTCA CGGACTCTGC CTACGTGGTG 
GCAAGCATGC GGATTATGAC TCGGTTGGGG ACACCTGTGC TTCAGGCCCT GGGAGATGGC 
GACTTTGTCA AGTGTCTTCA CTCTGTGGGC CAACCCTTGA CTGGGCAAGG GGAGCCGGTG 
AGCCAGTGGC CATGCAATCC AGAGAAAACC CTGATTGGCC ACGTGCCTGA CCAGCGGGAG 
ATCATCTCCT TCGGCAGCGG CTATGGTGGC AACTCCCTGC TGGGCAAGAA GTGCTTTGCC 
CTTCGCATCG CCTCTCGGCT GGCCCGGGAT GAGGGCTGGC TGGCGGAGCA CATGCTGATC 
CTAGGTATCA CCAATCCAGC GGGGAAGAAG CGCTATGTGG CAGCCGCCTT CCCCAGTGCT 
TGTGGCAAGA CAAACCTGGC CATGATGCGG CCGGCACTGC CAGGCTGGAA AGTGGAGTGT 
GTGGGGGATG ACATCGCCTG GATGAGGTTT GACAGTGATG GTCGACTCCG GGCCATCAAC 
CCTGAGAATG GCTTCTTTGG GGTGGCCCCT GGCACCTCTG CCACCACCAA CCCCAATGCT 
ATGGCCACAA TCCAGAGTAA CACCATTTTC ACCAACGTGG CTGAGACCAG CGATGGCGGC 
GTGTATTGGG AGGGCATTGA TCAGCCTCTT CCACCTGGCA TCACCGTGAC CTCCTGGCTA 
GGCAAACCCT GGAAACCTGG TGACAAGGAG CCCTGTGCGC ATCCCAACTC TCGCTTTTGT 
GCCCCGGCTC GCCAGTGCCC CATCATAGAC CCCGCCTGGG AGGCCCCAGA GGGAGTCCCC 
ATTGACGCCA TCATCTTTGG AGGCCGTAGA CCCAAAGGAG TCCCCCTGGT ATATGAGGCC 
TTCAACTGGC GCCACGGGGT GTTTGTGGGC AGTGCCATGC GCTCCGAGGC CACTGCAGCG 
GCTGAACACA AAGGGAAGGT CATCATGCAT GACCCATTTG CCATGCGGCC GTTTTTCGGC 
TACAACTTTG GGCGCTACCT TGAACACTGG CTGAGCATGG AGGGGCGCAA GGGGGTCCAG 
CTGCCCCGCA TCTTCCACGT CAACTGGTTC CGGCGCGACG AGGCAGGCCG CTTCCTGTGG 
CCAGGCTTTG GAGAGAATGC CCGGGTGCTA GACTGGATCT GCCGACGGCT CGACGGGGAG 
GAGAGTGCCC GGGAGACACC CATCGGACTG GTGCCTAAGG AAGGCGCCTT GGATCTCAGC 
AGCCTGGGAG CCATTGACAC CACCCAGTTG TTCTCCCTCC CCAAGGACTT TTGGCAACAG 
GAGGTTCATG ACATTCGGAG CTATCTGACA GAACAGGTCA ACCAGGATCT GCCCAAGGAA 
GTGCTGGCTG AACTGGAGGC CTTGGAGGGA CGTGTGCGCA GAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853059.1
CDS	320..1867
Translation	MGPRRRTLPHWPCWNSRDSSENSLSTTIGRTMYVIPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITNPAGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSDGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGITVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIIDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGRYLEHWLSMEGRKGVQLPRIFHVNWFRRDEAGRFLWPGFGENARVLDWICRRLDGEESARETPIGLVPKEGALDLSSLGAIDTTQLFSLPKDFWQQEVHDIRSYLTEQVNQDLPKEVLAELEALEGRVRRM

Target ORF information:

RefSeq Version	XM_024997291.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphoenolpyruvate carboxykinase 2, mitochondrial (PCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997291.1

ORF Insert Sequence:

ATGGGACCGA GGCGGAGAAC ACTGCCACAC TGGCCCTGCT GGAACAGCAG GGACTCATCC 
GAAAACTCCC TAAGTACAAC AATTGGCCGA ACCATGTACG TGATTCCGTT CAGCATGGGT 
CCCGTGGGCT CCCCGCTGTC CCGCATCGGA GTGCAGCTCA CGGACTCTGC CTACGTGGTG 
GCAAGCATGC GGATTATGAC TCGGTTGGGG ACACCTGTGC TTCAGGCCCT GGGAGATGGC 
GACTTTGTCA AGTGTCTTCA CTCTGTGGGC CAACCCTTGA CTGGGCAAGG GGAGCCGGTG 
AGCCAGTGGC CATGCAATCC AGAGAAAACC CTGATTGGCC ACGTGCCTGA CCAGCGGGAG 
ATCATCTCCT TCGGCAGCGG CTATGGTGGC AACTCCCTGC TGGGCAAGAA GTGCTTTGCC 
CTTCGCATCG CCTCTCGGCT GGCCCGGGAT GAGGGCTGGC TGGCGGAGCA CATGCTGATC 
CTAGGTATCA CCAATCCAGC GGGGAAGAAG CGCTATGTGG CAGCCGCCTT CCCCAGTGCT 
TGTGGCAAGA CAAACCTGGC CATGATGCGG CCGGCACTGC CAGGCTGGAA AGTGGAGTGT 
GTGGGGGATG ACATCGCCTG GATGAGGTTT GACAGTGATG GTCGACTCCG GGCCATCAAC 
CCTGAGAATG GCTTCTTTGG GGTGGCCCCT GGCACCTCTG CCACCACCAA CCCCAATGCT 
ATGGCCACAA TCCAGAGTAA CACCATTTTC ACCAACGTGG CTGAGACCAG CGATGGCGGC 
GTGTATTGGG AGGGCATTGA TCAGCCTCTT CCACCTGGCA TCACCGTGAC CTCCTGGCTA 
GGCAAACCCT GGAAACCTGG TGACAAGGAG CCCTGTGCGC ATCCCAACTC TCGCTTTTGT 
GCCCCGGCTC GCCAGTGCCC CATCATAGAC CCCGCCTGGG AGGCCCCAGA GGGAGTCCCC 
ATTGACGCCA TCATCTTTGG AGGCCGTAGA CCCAAAGGAG TCCCCCTGGT ATATGAGGCC 
TTCAACTGGC GCCACGGGGT GTTTGTGGGC AGTGCCATGC GCTCCGAGGC CACTGCAGCG 
GCTGAACACA AAGGGAAGGT CATCATGCAT GACCCATTTG CCATGCGGCC GTTTTTCGGC 
TACAACTTTG GGCGCTACCT TGAACACTGG CTGAGCATGG AGGGGCGCAA GGGGGTCCAG 
CTGCCCCGCA TCTTCCACGT CAACTGGTTC CGGCGCGACG AGGCAGGCCG CTTCCTGTGG 
CCAGGCTTTG GAGAGAATGC CCGGGTGCTA GACTGGATCT GCCGACGGCT CGACGGGGAG 
GAGAGTGCCC GGGAGACACC CATCGGACTG GTGCCTAAGG AAGGCGCCTT GGATCTCAGC 
AGCCTGGGAG CCATTGACAC CACCCAGTTG TTCTCCCTCC CCAAGGACTT TTGGCAACAG 
GAGGTTCATG ACATTCGGAG CTATCTGACA GAACAGGTCA ACCAGGATCT GCCCAAGGAA 
GTGCTGGCTG AACTGGAGGC CTTGGAGGGA CGTGTGCGCA GAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cloning and characterization of bovine cytosolic and mitochondrial PEPCK during transition to lactation.
Physiological genomics11(2)53-63(2002 Oct)
Agca C,Greenfield RB,Hartwell JR,Donkin SS