HMGB1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HMGB1
Entrez Gene ID	282691
Full Name	high mobility group box 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005213558.1	XP_005213615.1	high mobility group protein B1 isoform X1
XM_010810664.2	XP_010808966.1	high mobility group protein B1 isoform X1
NM_176612.1	NP_788785.1	high mobility group protein B1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	HMGB1	NP_788785.1
Gallus gallus (chicken)	HMGB1	NP_990233.1
	HMGB1	XP_001100203.1
Canis lupus familiaris (dog)	HMGB1	NP_001002937.1
Rattus norvegicus (Norway rat)	LOC685520	XP_003749961.1
Pan troglodytes (chimpanzee)	HMGB1	XP_509611.1
Danio rerio (zebrafish)	hmgb1a	NP_955849.2
Homo sapiens (human)	HMGB1	NP_002119.1

Gene Ontology
Bibliography

Related articles in PubMed

Synergistic effect of high-mobility group box-1 and lipopolysaccharide on cytokine induction in bovine peripheral blood mononuclear cells.
Appavoo E, Hajam IA, Muneeswaran NS, Kondabattula G, Bhanuprakash V, Kishore S
Microbiology and immunology60(3)196-202(2016 Mar)

One SNP in the 3'-UTR of HMGB1 gene affects the binding of target bta-miR-223 and is involved in mastitis in dairy cattle.
Li L, Huang J, Zhang X, Ju Z, Qi C, Zhang Y, Li Q, Wang C, Miao W, Zhong J, Hou M, Hang S
Immunogenetics64(11)817-24(2012 Nov)

[Conformational peculiarities of nuclear protein HMGB1 and specificity of its interaction with DNA].
Polianichko AM, Rodionova TIu, Vorob'ev VI, Chikhirzhina EV
Tsitologiia53(1)55-60(2011)

[Interaction between non-histone chromatin protein HMGB1 and linker histone H1].
Fonin AV, Stepanenko OV, Turoverov KK, Vorob'ev VI
Tsitologiia52(11)946-9(2010)

The primary structures of non-histone chromosomal proteins HMG 1 and 2.
Walker JM, Gooderham K, Hastings JR, Mayes E, Johns EW
FEBS letters122(2)264-70(1980 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HMGB1 can act as an adjuvant in modulating the bovine immune system and thus lays a foundation for using HMGB1 as an adjuvant in various bovine vaccine preparations.
Title: Synergistic effect of high-mobility group box-1 and lipopolysaccharide on cytokine induction in bovine peripheral blood mononuclear cells.

Single-nucleotide polymorphism in the 3'-untranslated region of the HMGB1 gene affects the binding of target bta-miR-223 and is involved in mastitis.
Title: One SNP in the 3'-UTR of HMGB1 gene affects the binding of target bta-miR-223 and is involved in mastitis in dairy cattle.

The mechanisms of interaction of the non-histone chromosomal protein HMGB1 and linker histone H1 with DNA have been studied using circular dichroism and absorption spectroscopy.
Title: [The structure of the complexes of DNA with chromosomal protein HMGB1 and histone H1 in the presence of manganese ions. I. Circular dicroism spectroscopy].

HMGB1 is able to induce considerable changes in DNA structure upon binding even when the amount of the protein directly associated with DNA is low
Title: [Conformational peculiarities of nuclear protein HMGB1 and specificity of its interaction with DNA].

Interaction between non-histone chromatin protein HMGB1 and linker histone H1
Title: [Interaction between non-histone chromatin protein HMGB1 and linker histone H1].

The following HMGB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HMGB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41492	NM_176612.1 Latest version!	Bos taurus high mobility group box 1 (HMGB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OBa24618	XM_010810664.2 Latest version!	Bos taurus high mobility group box 1 (HMGB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OBa24618	XM_005213558.1 Latest version!	Bos taurus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HMGB1 ( NM_176612.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_788785.1 Bos taurus high mobility group box 1 (HMGB1), mRNA.

Price & Availability↑

CloneID	OBa41492
Clone ID Related Accession (Same CDS sequence)	NM_176612.1
Accession Version	NM_176612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	high mobility group protein B1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X12796.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X12796.1, BC102929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAAAG GAGATCCTAA GAAGCCGAGA GGCAAAATGT CATCATATGC ATTCTTTGTG 
CAAACTTGCC GGGAGGAGCA CAAGAAGAAG CACCCGGATG CTTCTGTCAA CTTCTCAGAG 
TTTTCTAAGA AGTGCTCAGA GAGGTGGAAG ACCATGTCTG CTAAAGAGAA AGGAAAATTT 
GAAGACATGG CAAAGGCGGA CAAGGCCCGT TATGAAAGAG AAATGAAAAC TTATATTCCT 
CCTAAAGGGG AAACAAAAAA GAAGTTCAAG GATCCCAATG CACCTAAGAG GCCTCCTTCG 
GCCTTTTTCT TGTTTTGTTC TGAGTATCGT CCAAAAATCA AAGGCGAACA TCCTGGCCTG 
TCTATTGGTG ATGTTGCAAA GAAACTGGGA GAGATGTGGA ATAACACTGC TGCGGATGAT 
AAGCAGCCTT ATGAGAAGAA GGCTGCTAAG CTGAAGGAAA AGTATGAAAA GGATATTGCC 
GCATACCGAG CTAAAGGGAA ACCTGATGCA GCAAAAAAGG GAGTTGTCAA GGCTGAAAAG 
AGCAAGAAAA AGAAGGAAGA GGAGGAAGAT GAGGAAGATG AAGAGGATGA GGAGGAGGAA 
GAAGATGAAG AGGATGAGGA GGAGGAAGAA GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788785.1
CDS	119..766
Translation	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEEEEEDDDDE

Target ORF information:

RefSeq Version	NM_176612.1
Organism	Bos taurus(cattle)
Definition	Bos taurus high mobility group box 1 (HMGB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176612.1

ORF Insert Sequence:

ATGGGCAAAG GAGATCCTAA GAAGCCGAGA GGCAAAATGT CATCATATGC ATTCTTTGTG 
CAAACTTGCC GGGAGGAGCA CAAGAAGAAG CACCCGGATG CTTCTGTCAA CTTCTCAGAG 
TTTTCTAAGA AGTGCTCAGA GAGGTGGAAG ACCATGTCTG CTAAAGAGAA AGGAAAATTT 
GAAGACATGG CAAAGGCGGA CAAGGCCCGT TATGAAAGAG AAATGAAAAC TTATATTCCT 
CCTAAAGGGG AAACAAAAAA GAAGTTCAAG GATCCCAATG CACCTAAGAG GCCTCCTTCG 
GCCTTTTTCT TGTTTTGTTC TGAGTATCGT CCAAAAATCA AAGGCGAACA TCCTGGCCTG 
TCTATTGGTG ATGTTGCAAA GAAACTGGGA GAGATGTGGA ATAACACTGC TGCGGATGAT 
AAGCAGCCTT ATGAGAAGAA GGCTGCTAAG CTGAAGGAAA AGTATGAAAA GGATATTGCC 
GCATACCGAG CTAAAGGGAA ACCTGATGCA GCAAAAAAGG GAGTTGTCAA GGCTGAAAAG 
AGCAAGAAAA AGAAGGAAGA GGAGGAAGAT GAGGAAGATG AAGAGGATGA GGAGGAGGAA 
GAAGATGAAG AGGATGAGGA GGAGGAAGAA GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGB1 ( XM_005213558.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005213615.1 Bos taurus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa24618
Clone ID Related Accession (Same CDS sequence)	XM_005213558.1 , XM_010810664.2
Accession Version	XM_005213558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	high mobility group protein B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAAG GAGATCCTAA GAAGCCGAGA GGCAAAATGT CATCATATGC ATTCTTTGTG 
CAAACTTGCC GGGAGGAGCA CAAGAAGAAG CACCCGGATG CTTCTGTCAA CTTCTCAGAG 
TTTTCTAAGA AGTGCTCAGA GAGGTGGAAG ACCATGTCTG CTAAAGAGAA AGGAAAATTT 
GAAGACATGG CAAAGGCGGA CAAGGCCCGT TATGAAAGAG AAATGAAAAC TTATATTCCT 
CCTAAAGGGG AAACAAAAAA GAAGTTCAAG GATCCCAATG CACCTAAGAG GCCTCCTTCG 
GCCTTTTTCT TGTTTTGTTC TGAGTATCGT CCAAAAATCA AAGGCGAACA TCCTGGCCTG 
TCTATTGGTG ATGTTGCAAA GAAACTGGGA GAGATGTGGA ATAACACTGC TGCGGATGAT 
AAGCAGCCTT ATGAGAAGAA GGCTGCTAAG CTGAAGGAAA AGTATGAAAA GGATATTGCC 
GCATACCGAG CTAAAGGGAA GCCTGATGCA GCAAAAAAGG GAGTTGTCAA GGCTGAAAAG 
AGCAAGAAAA AGAAGGAAGA GGAGGAAGAT GAGGAAGATG AAGAGGATGA GGAGGAGGAA 
GAAGATGAAG AGGATGAGGA GGAGGAAGAA GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005213615.1
CDS	217..864
Translation	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEEEEEDDDDE

Target ORF information:

RefSeq Version	XM_005213558.1
Organism	Bos taurus(cattle)
Definition	Bos taurus high mobility group box 1 (HMGB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005213558.1

ORF Insert Sequence:

ATGGGCAAAG GAGATCCTAA GAAGCCGAGA GGCAAAATGT CATCATATGC ATTCTTTGTG 
CAAACTTGCC GGGAGGAGCA CAAGAAGAAG CACCCGGATG CTTCTGTCAA CTTCTCAGAG 
TTTTCTAAGA AGTGCTCAGA GAGGTGGAAG ACCATGTCTG CTAAAGAGAA AGGAAAATTT 
GAAGACATGG CAAAGGCGGA CAAGGCCCGT TATGAAAGAG AAATGAAAAC TTATATTCCT 
CCTAAAGGGG AAACAAAAAA GAAGTTCAAG GATCCCAATG CACCTAAGAG GCCTCCTTCG 
GCCTTTTTCT TGTTTTGTTC TGAGTATCGT CCAAAAATCA AAGGCGAACA TCCTGGCCTG 
TCTATTGGTG ATGTTGCAAA GAAACTGGGA GAGATGTGGA ATAACACTGC TGCGGATGAT 
AAGCAGCCTT ATGAGAAGAA GGCTGCTAAG CTGAAGGAAA AGTATGAAAA GGATATTGCC 
GCATACCGAG CTAAAGGGAA GCCTGATGCA GCAAAAAAGG GAGTTGTCAA GGCTGAAAAG 
AGCAAGAAAA AGAAGGAAGA GGAGGAAGAT GAGGAAGATG AAGAGGATGA GGAGGAGGAA 
GAAGATGAAG AGGATGAGGA GGAGGAAGAA GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HMGB1 ( XM_010810664.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808966.1 Bos taurus high mobility group box 1 (HMGB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa24618
Clone ID Related Accession (Same CDS sequence)	XM_005213558.1 , XM_010810664.2
Accession Version	XM_010810664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	high mobility group protein B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104311.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAAG GAGATCCTAA GAAGCCGAGA GGCAAAATGT CATCATATGC ATTCTTTGTG 
CAAACTTGCC GGGAGGAGCA CAAGAAGAAG CACCCGGATG CTTCTGTCAA CTTCTCAGAG 
TTTTCTAAGA AGTGCTCAGA GAGGTGGAAG ACCATGTCTG CTAAAGAGAA AGGAAAATTT 
GAAGACATGG CAAAGGCGGA CAAGGCCCGT TATGAAAGAG AAATGAAAAC TTATATTCCT 
CCTAAAGGGG AAACAAAAAA GAAGTTCAAG GATCCCAATG CACCTAAGAG GCCTCCTTCG 
GCCTTTTTCT TGTTTTGTTC TGAGTATCGT CCAAAAATCA AAGGCGAACA TCCTGGCCTG 
TCTATTGGTG ATGTTGCAAA GAAACTGGGA GAGATGTGGA ATAACACTGC TGCGGATGAT 
AAGCAGCCTT ATGAGAAGAA GGCTGCTAAG CTGAAGGAAA AGTATGAAAA GGATATTGCC 
GCATACCGAG CTAAAGGGAA GCCTGATGCA GCAAAAAAGG GAGTTGTCAA GGCTGAAAAG 
AGCAAGAAAA AGAAGGAAGA GGAGGAAGAT GAGGAAGATG AAGAGGATGA GGAGGAGGAA 
GAAGATGAAG AGGATGAGGA GGAGGAAGAA GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010808966.1
CDS	362..1009
Translation	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEEEEEDDDDE

Target ORF information:

RefSeq Version	XM_010810664.2
Organism	Bos taurus(cattle)
Definition	Bos taurus high mobility group box 1 (HMGB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010810664.2

ORF Insert Sequence:

ATGGGCAAAG GAGATCCTAA GAAGCCGAGA GGCAAAATGT CATCATATGC ATTCTTTGTG 
CAAACTTGCC GGGAGGAGCA CAAGAAGAAG CACCCGGATG CTTCTGTCAA CTTCTCAGAG 
TTTTCTAAGA AGTGCTCAGA GAGGTGGAAG ACCATGTCTG CTAAAGAGAA AGGAAAATTT 
GAAGACATGG CAAAGGCGGA CAAGGCCCGT TATGAAAGAG AAATGAAAAC TTATATTCCT 
CCTAAAGGGG AAACAAAAAA GAAGTTCAAG GATCCCAATG CACCTAAGAG GCCTCCTTCG 
GCCTTTTTCT TGTTTTGTTC TGAGTATCGT CCAAAAATCA AAGGCGAACA TCCTGGCCTG 
TCTATTGGTG ATGTTGCAAA GAAACTGGGA GAGATGTGGA ATAACACTGC TGCGGATGAT 
AAGCAGCCTT ATGAGAAGAA GGCTGCTAAG CTGAAGGAAA AGTATGAAAA GGATATTGCC 
GCATACCGAG CTAAAGGGAA GCCTGATGCA GCAAAAAAGG GAGTTGTCAA GGCTGAAAAG 
AGCAAGAAAA AGAAGGAAGA GGAGGAAGAT GAGGAAGATG AAGAGGATGA GGAGGAGGAA 
GAAGATGAAG AGGATGAGGA GGAGGAAGAA GATGATGATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Synergistic effect of high-mobility group box-1 and lipopolysaccharide on cytokine induction in bovine peripheral blood mononuclear cells.
Microbiology and immunology60(3)196-202(2016 Mar)
Appavoo E,Hajam IA,Muneeswaran NS,Kondabattula G,Bhanuprakash V,Kishore S

One SNP in the 3'-UTR of HMGB1 gene affects the binding of target bta-miR-223 and is involved in mastitis in dairy cattle.
Immunogenetics64(11)817-24(2012 Nov)
Li L,Huang J,Zhang X,Ju Z,Qi C,Zhang Y,Li Q,Wang C,Miao W,Zhong J,Hou M,Hang S

[Conformational peculiarities of nuclear protein HMGB1 and specificity of its interaction with DNA].
Tsitologiia53(1)55-60(2011)
Polianichko AM,Rodionova TIu,Vorob'ev VI,Chikhirzhina EV

[Interaction between non-histone chromatin protein HMGB1 and linker histone H1].
Tsitologiia52(11)946-9(2010)
Fonin AV,Stepanenko OV,Turoverov KK,Vorob'ev VI

The primary structures of non-histone chromosomal proteins HMG 1 and 2.
FEBS letters122(2)264-70(1980 Dec)
Walker JM,Gooderham K,Hastings JR,Mayes E,Johns EW

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HMGB1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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