ATP5F1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP5F1A
Entrez Gene ID	282578
Full Name	ATP synthase F1 subunit alpha
Synonyms	ATP5A1
General protein information	Preferred Names ATP synthase F1 subunit alpha Names ATP synthase subunit alpha, mitochondrial ATP synthase alpha subunit ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984181.1	XP_024839949.1	ATP synthase subunit alpha, mitochondrial isoform X1
XM_024984182.1	XP_024839950.1	ATP synthase subunit alpha, mitochondrial isoform X1
NM_174684.2	NP_777109.1	ATP synthase subunit alpha, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ATP5A1	NP_001129208.1
Mus musculus (house mouse)	Atp5a1	NP_031531.1
Saccharomyces cerevisiae (baker's yeast)	ATP1	NP_009453.2
Schizosaccharomyces pombe (fission yeast)	atp1	NP_594919.1
Magnaporthe oryzae (rice blast fungus)	MGG_07752	XP_003712965.1
Neurospora crassa	NCU02514	XP_965645.1
Canis lupus familiaris (dog)	ATP5A1	XP_852877.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005134	XP_314018.2
	ATP5A1	XP_001089681.2
Bos taurus (cattle)	ATP5A1	NP_777109.1
Caenorhabditis elegans (roundworm)	H28O16.1	NP_001021526.1
Arabidopsis thaliana (thale cress)	AT2G07698	NP_178788.1
Arabidopsis thaliana (thale cress)	atp1	NP_085571.2
Homo sapiens (human)	ATP5A1	NP_004037.1
Rattus norvegicus (Norway rat)	Atp5a1	NP_075581.1
Danio rerio (zebrafish)	atp5a1	NP_001070823.1
Kluyveromyces lactis	KLLA0E06667g	XP_454248.1
Gallus gallus (chicken)	ATP5A1	XP_004949099.1
Gallus gallus (chicken)	ATP5A1W	XP_429118.3
Drosophila melanogaster (fruit fly)	blw	NP_726243.1
Eremothecium gossypii	AGOS_AGL272C	NP_986395.1
Xenopus tropicalis (tropical clawed frog)	atp5a1	NP_001025610.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM.
Zhou A, Rohou A, Schep DG, Bason JV, Montgomery MG, Walker JE, Grigorieff N, Rubinstein JL
eLife4e10180(2015 Oct)

Identification of the F1-ATPase at the cell surface of colonic epithelial cells: role in mediating cell proliferation.
Kowalski-Chauvel A, Najib S, Tikhonova IG, Huc L, Lopez F, Martinez LO, Cohen-Jonathan-Moyal E, Ferrand A, Seva C
The Journal of biological chemistry287(49)41458-68(2012 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Mechanism of inhibition of bovine F1-ATPase by resveratrol and related polyphenols.
Gledhill JR, Montgomery MG, Leslie AG, Walker JE
Proceedings of the National Academy of Sciences of the United States of America104(34)13632-7(2007 Aug)

Association of two proteolipids of unknown function with ATP synthase from bovine heart mitochondria.
Chen R, Runswick MJ, Carroll J, Fearnley IM, Walker JE
FEBS letters581(17)3145-8(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Combining cryoelectron microscopy data with bioinformatic analysis allowed the authors to determine the fold of the a subunit, suggesting a proton translocation path through the FO region that involves both the a and b subunits.
Title: Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM.

F1-ATPase at the cell surface of colonic epithelial cells has a role in mediating cell proliferation
Title: Identification of the F1-ATPase at the cell surface of colonic epithelial cells: role in mediating cell proliferation.

The beneficial effects of dietary resveratrol may derive in part by preventing mitochondrial ATP synthesis in tumor cells, thereby inducing apoptosis.
Title: Mechanism of inhibition of bovine F1-ATPase by resveratrol and related polyphenols.

Nuclear magnetic resonance characterization of the interaction between OSCP-N terminal and a peptide corresponding to residues 1-25 of the alpha-subunit of bovine F(1)-ATPase is presented.
Title: How the N-terminal domain of the OSCP subunit of bovine F1Fo-ATP synthase interacts with the N-terminal region of an alpha subunit.

The following ATP5F1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP5F1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43896	NM_174684.2 Latest version!	Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa32493	XM_024984182.1 Latest version!	Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OBa32493	XM_024984181.1 Latest version!	Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP5F1A ( NM_174684.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777109.1 Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), mRNA.

Price & Availability↑

CloneID	OBa43896
Clone ID Related Accession (Same CDS sequence)	NM_174684.2
Accession Version	NM_174684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	ATP synthase subunit alpha, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M22465.1. On Jun 10, 2007 this sequence version replaced NM_174684.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M22465.1, BC116059.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTGTCCG TGCGCGTCGC CGCGGCGGTG GCCCGCGCCC TCCCTCGGCG AGCGGGGCTG 
GTCTCCAAAA ATGCTTTGGG ATCATCCTTC ATTGCTGCAA GGAACCTGCA TGCCTCTAAC 
TCTCGTCTGC AGAAGACCGG CACTGCTGAG GTGTCCTCTA TTCTTGAAGA GCGTATTCTT 
GGAGCTGATA CCTCTGTTGA CCTTGAAGAG ACTGGGCGTG TTTTAAGTAT TGGTGATGGT 
ATTGCTCGGG TACATGGGCT AAGAAATGTT CAAGCAGAAG AAATGGTAGA GTTTTCTTCA 
GGTTTAAAGG GTATGTCTTT GAACTTGGAG CCTGACAATG TTGGTGTTGT CGTGTTTGGA 
AATGATAAAC TTATTAAGGA AGGAGATATT GTGAAGAGAA CTGGGGCTAT TGTAGATGTT 
CCAGTCGGCG AGGAGCTGCT GGGTCGTGTA GTTGATGCCC TTGGTAATGC CATTGATGGA 
AAGGGTCCAA TTGGTTCCAA GGCCCGAAGA CGAGTTGGCT TGAAAGCCCC TGGGATCATT 
CCTCGAATCT CTGTGAGGGA ACCAATGCAA ACTGGCATTA AGGCTGTGGA TAGCTTGGTG 
CCGATTGGTC GTGGTCAGCG TGAGCTGATT ATTGGTGACC GACAGACTGG CAAAACGTCA 
ATTGCAATTG ACACAATCAT TAACCAGAAA CGATTCAATG ATGGAACTGA TGAAAAGAAG 
AAGCTGTACT GTATCTATGT TGCTATTGGT CAAAAGAGAT CCACTGTTGC CCAGTTGGTG 
AAGAGACTTA CAGATGCAGA TGCCATGAAG TATACCATTG TGGTTTCAGC TACTGCTTCA 
GATGCTGCCC CACTTCAGTA CTTGGCTCCT TATTCTGGAT GTTCAATGGG AGAATATTTT 
AGGGATAATG GCAAACACGC TTTGATCATT TATGATGACT TATCCAAACA GGCGGTTGCT 
TATCGTCAGA TGTCTCTGCT GCTCCGCCGA CCCCCTGGTC GTGAGGCCTA TCCTGGTGAT 
GTATTCTACC TGCACTCCCG ACTCCTGGAG AGAGCAGCTA AAATGAACGA TGCTTTTGGT 
GGTGGCTCCT TGACTGCTCT ACCAGTCATC GAAACACAAG CTGGTGATGT GTCTGCTTAC 
ATTCCAACAA ATGTCATTTC CATCACTGAC GGACAGATCT TCTTGGAAAC AGAATTGTTC 
TACAAAGGTA TCCGCCCTGC CATTAATGTT GGTTTGTCTG TGTCCCGTGT CGGATCTGCT 
GCCCAAACCA GGGCCATGAA ACAGGTGGCA GGTACTATGA AACTGGAGTT GGCTCAGTAT 
CGTGAGGTTG CTGCTTTTGC CCAGTTCGGT TCTGACCTTG ATGCTGCCAC TCAACAACTC 
TTGAGTCGTG GTGTGCGTTT GACTGAGCTG CTCAAGCAGG GACAGTATTC TCCCATGGCT 
ATTGAAGAGC AAGTGGCTGT TATCTATGCG GGTGTCAGGG GGTATCTTGA CAAGCTGGAG 
CCCAGCAAGA TCACAAAATT TGAGAATGCT TTCTTGTCTC ACGTTATCAG CCAACATCAG 
GCCCTGTTGA GCAAAATCAG GACCGATGGA AAGATCTCAG AAGAGTCAGA TGCAAAGCTG 
AAAGAGATTG TAACAAACTT CTTGGCTGGA TTTGAAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777109.1
CDS	21..1682
Translation	MLSVRVAAAVARALPRRAGLVSKNALGSSFIAARNLHASNSRLQKTGTAEVSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKARRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVISQHQALLSKIRTDGKISEESDAKLKEIVTNFLAGFEA

Target ORF information:

RefSeq Version	NM_174684.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174684.2

ORF Insert Sequence:

ATGCTGTCCG TGCGCGTCGC CGCGGCGGTG GCCCGCGCCC TCCCTCGGCG AGCGGGGCTG 
GTCTCCAAAA ATGCTTTGGG ATCATCCTTC ATTGCTGCAA GGAACCTGCA TGCCTCTAAC 
TCTCGTCTGC AGAAGACCGG CACTGCTGAG GTGTCCTCTA TTCTTGAAGA GCGTATTCTT 
GGAGCTGATA CCTCTGTTGA CCTTGAAGAG ACTGGGCGTG TTTTAAGTAT TGGTGATGGT 
ATTGCTCGGG TACATGGGCT AAGAAATGTT CAAGCAGAAG AAATGGTAGA GTTTTCTTCA 
GGTTTAAAGG GTATGTCTTT GAACTTGGAG CCTGACAATG TTGGTGTTGT CGTGTTTGGA 
AATGATAAAC TTATTAAGGA AGGAGATATT GTGAAGAGAA CTGGGGCTAT TGTAGATGTT 
CCAGTCGGCG AGGAGCTGCT GGGTCGTGTA GTTGATGCCC TTGGTAATGC CATTGATGGA 
AAGGGTCCAA TTGGTTCCAA GGCCCGAAGA CGAGTTGGCT TGAAAGCCCC TGGGATCATT 
CCTCGAATCT CTGTGAGGGA ACCAATGCAA ACTGGCATTA AGGCTGTGGA TAGCTTGGTG 
CCGATTGGTC GTGGTCAGCG TGAGCTGATT ATTGGTGACC GACAGACTGG CAAAACGTCA 
ATTGCAATTG ACACAATCAT TAACCAGAAA CGATTCAATG ATGGAACTGA TGAAAAGAAG 
AAGCTGTACT GTATCTATGT TGCTATTGGT CAAAAGAGAT CCACTGTTGC CCAGTTGGTG 
AAGAGACTTA CAGATGCAGA TGCCATGAAG TATACCATTG TGGTTTCAGC TACTGCTTCA 
GATGCTGCCC CACTTCAGTA CTTGGCTCCT TATTCTGGAT GTTCAATGGG AGAATATTTT 
AGGGATAATG GCAAACACGC TTTGATCATT TATGATGACT TATCCAAACA GGCGGTTGCT 
TATCGTCAGA TGTCTCTGCT GCTCCGCCGA CCCCCTGGTC GTGAGGCCTA TCCTGGTGAT 
GTATTCTACC TGCACTCCCG ACTCCTGGAG AGAGCAGCTA AAATGAACGA TGCTTTTGGT 
GGTGGCTCCT TGACTGCTCT ACCAGTCATC GAAACACAAG CTGGTGATGT GTCTGCTTAC 
ATTCCAACAA ATGTCATTTC CATCACTGAC GGACAGATCT TCTTGGAAAC AGAATTGTTC 
TACAAAGGTA TCCGCCCTGC CATTAATGTT GGTTTGTCTG TGTCCCGTGT CGGATCTGCT 
GCCCAAACCA GGGCCATGAA ACAGGTGGCA GGTACTATGA AACTGGAGTT GGCTCAGTAT 
CGTGAGGTTG CTGCTTTTGC CCAGTTCGGT TCTGACCTTG ATGCTGCCAC TCAACAACTC 
TTGAGTCGTG GTGTGCGTTT GACTGAGCTG CTCAAGCAGG GACAGTATTC TCCCATGGCT 
ATTGAAGAGC AAGTGGCTGT TATCTATGCG GGTGTCAGGG GGTATCTTGA CAAGCTGGAG 
CCCAGCAAGA TCACAAAATT TGAGAATGCT TTCTTGTCTC ACGTTATCAG CCAACATCAG 
GCCCTGTTGA GCAAAATCAG GACCGATGGA AAGATCTCAG AAGAGTCAGA TGCAAAGCTG 
AAAGAGATTG TAACAAACTT CTTGGCTGGA TTTGAAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5F1A ( XM_024984181.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839949.1 Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32493
Clone ID Related Accession (Same CDS sequence)	XM_024984181.1 , XM_024984182.1
Accession Version	XM_024984181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP synthase subunit alpha, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCG TGCGCGTCGC CGCGGCCGTG GCCCGCGCCC TCCCTCGGCG AGCGGGGCTG 
GTCTCCAAAA ATGCTTTGGG ATCATCCTTC ATTGCTGCAA GGAACCTGCA TGCCTCTAAC 
TCTCGTCTGC AGAAGACCGG CACTGCTGAG GTGTCCTCTA TTCTTGAAGA GCGTATTCTT 
GGAGCTGATA CCTCTGTTGA CCTTGAAGAG ACTGGGCGTG TTTTAAGTAT TGGTGATGGT 
ATTGCTCGGG TACATGGGCT AAGAAATGTT CAAGCAGAAG AAATGGTAGA GTTTTCTTCA 
GGTTTAAAGG GTATGTCTTT GAACTTGGAG CCTGACAATG TTGGTGTTGT CGTGTTTGGA 
AATGATAAAC TTATTAAGGA AGGAGATATT GTGAAGAGAA CTGGGGCTAT TGTAGATGTT 
CCAGTCGGCG AGGAGCTGCT GGGTCGTGTA GTTGATGCCC TTGGTAATGC CATTGATGGA 
AAGGGTCCAA TTGGTTCCAA GGCCCGAAGA CGAGTTGGCT TGAAAGCCCC TGGGATCATT 
CCTCGAATCT CTGTGAGGGA ACCAATGCAA ACTGGCATTA AGGCTGTGGA TAGCTTGGTG 
CCGATTGGTC GTGGTCAGCG TGAGCTGATT ATTGGTGACC GACAGACTGG CAAAACGTCA 
ATTGCAATTG ACACAATCAT TAACCAGAAA CGATTCAATG ATGGAACTGA TGAAAAGAAG 
AAGCTGTACT GTATCTATGT TGCTATTGGT CAAAAGAGAT CCACTGTTGC CCAGTTGGTG 
AAGAGACTTA CAGATGCAGA TGCCATGAAG TATACCATTG TGGTTTCAGC TACTGCTTCA 
GATGCTGCCC CACTTCAGTA CTTGGCTCCT TATTCTGGAT GTTCAATGGG AGAATATTTT 
AGGGATAATG GCAAACACGC TTTGATCATT TATGATGACT TATCCAAACA GGCGGTTGCT 
TATCGTCAGA TGTCTCTGCT GCTCCGCCGA CCCCCTGGTC GTGAGGCCTA TCCTGGTGAT 
GTATTCTACC TGCACTCCCG ACTCCTGGAG AGAGCAGCTA AAATGAACGA TGCTTTTGGT 
GGTGGCTCCT TGACTGCTCT ACCAGTCATC GAAACACAAG CTGGTGATGT GTCTGCTTAC 
ATTCCAACAA ATGTCATTTC CATCACTGAC GGACAGATCT TCTTGGAAAC AGAATTGTTC 
TACAAAGGTA TCCGCCCTGC CATTAATGTT GGTTTGTCTG TGTCCCGTGT CGGATCTGCT 
GCCCAAACCA GGGCCATGAA ACAGGTGGCA GGTACTATGA AACTGGAGTT GGCTCAGTAT 
CGTGAGGTTG CTGCTTTTGC CCAGTTCGGT TCTGACCTTG ATGCTGCCAC TCAACAACTC 
TTGAGTCGTG GTGTGCGTTT GACTGAGCTG CTCAAGCAGG GACAGTATTC TCCCATGGCT 
ATTGAAGAGC AAGTGGCTGT TATCTATGCG GGTGTCAGGG GGTATCTTGA CAAGCTGGAG 
CCCAGCAAGA TCACAAAATT TGAGAATGCT TTCTTGTCTC ACGTTATCAG CCAACATCAG 
GCCCTGTTGG GCAAAATCAG GACCGATGGA AAGATCTCAG AAGAGTCAGA TGCAAAGCTG 
AAAGAGATTG TAACAAACTT CTTGGCTGGA TTTGAAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839949.1
CDS	241..1902
Translation	MLSVRVAAAVARALPRRAGLVSKNALGSSFIAARNLHASNSRLQKTGTAEVSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKARRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVISQHQALLGKIRTDGKISEESDAKLKEIVTNFLAGFEA

Target ORF information:

RefSeq Version	XM_024984181.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984181.1

ORF Insert Sequence:

ATGCTGTCCG TGCGCGTCGC CGCGGCCGTG GCCCGCGCCC TCCCTCGGCG AGCGGGGCTG 
GTCTCCAAAA ATGCTTTGGG ATCATCCTTC ATTGCTGCAA GGAACCTGCA TGCCTCTAAC 
TCTCGTCTGC AGAAGACCGG CACTGCTGAG GTGTCCTCTA TTCTTGAAGA GCGTATTCTT 
GGAGCTGATA CCTCTGTTGA CCTTGAAGAG ACTGGGCGTG TTTTAAGTAT TGGTGATGGT 
ATTGCTCGGG TACATGGGCT AAGAAATGTT CAAGCAGAAG AAATGGTAGA GTTTTCTTCA 
GGTTTAAAGG GTATGTCTTT GAACTTGGAG CCTGACAATG TTGGTGTTGT CGTGTTTGGA 
AATGATAAAC TTATTAAGGA AGGAGATATT GTGAAGAGAA CTGGGGCTAT TGTAGATGTT 
CCAGTCGGCG AGGAGCTGCT GGGTCGTGTA GTTGATGCCC TTGGTAATGC CATTGATGGA 
AAGGGTCCAA TTGGTTCCAA GGCCCGAAGA CGAGTTGGCT TGAAAGCCCC TGGGATCATT 
CCTCGAATCT CTGTGAGGGA ACCAATGCAA ACTGGCATTA AGGCTGTGGA TAGCTTGGTG 
CCGATTGGTC GTGGTCAGCG TGAGCTGATT ATTGGTGACC GACAGACTGG CAAAACGTCA 
ATTGCAATTG ACACAATCAT TAACCAGAAA CGATTCAATG ATGGAACTGA TGAAAAGAAG 
AAGCTGTACT GTATCTATGT TGCTATTGGT CAAAAGAGAT CCACTGTTGC CCAGTTGGTG 
AAGAGACTTA CAGATGCAGA TGCCATGAAG TATACCATTG TGGTTTCAGC TACTGCTTCA 
GATGCTGCCC CACTTCAGTA CTTGGCTCCT TATTCTGGAT GTTCAATGGG AGAATATTTT 
AGGGATAATG GCAAACACGC TTTGATCATT TATGATGACT TATCCAAACA GGCGGTTGCT 
TATCGTCAGA TGTCTCTGCT GCTCCGCCGA CCCCCTGGTC GTGAGGCCTA TCCTGGTGAT 
GTATTCTACC TGCACTCCCG ACTCCTGGAG AGAGCAGCTA AAATGAACGA TGCTTTTGGT 
GGTGGCTCCT TGACTGCTCT ACCAGTCATC GAAACACAAG CTGGTGATGT GTCTGCTTAC 
ATTCCAACAA ATGTCATTTC CATCACTGAC GGACAGATCT TCTTGGAAAC AGAATTGTTC 
TACAAAGGTA TCCGCCCTGC CATTAATGTT GGTTTGTCTG TGTCCCGTGT CGGATCTGCT 
GCCCAAACCA GGGCCATGAA ACAGGTGGCA GGTACTATGA AACTGGAGTT GGCTCAGTAT 
CGTGAGGTTG CTGCTTTTGC CCAGTTCGGT TCTGACCTTG ATGCTGCCAC TCAACAACTC 
TTGAGTCGTG GTGTGCGTTT GACTGAGCTG CTCAAGCAGG GACAGTATTC TCCCATGGCT 
ATTGAAGAGC AAGTGGCTGT TATCTATGCG GGTGTCAGGG GGTATCTTGA CAAGCTGGAG 
CCCAGCAAGA TCACAAAATT TGAGAATGCT TTCTTGTCTC ACGTTATCAG CCAACATCAG 
GCCCTGTTGG GCAAAATCAG GACCGATGGA AAGATCTCAG AAGAGTCAGA TGCAAAGCTG 
AAAGAGATTG TAACAAACTT CTTGGCTGGA TTTGAAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5F1A ( XM_024984182.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839950.1 Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32493
Clone ID Related Accession (Same CDS sequence)	XM_024984181.1 , XM_024984182.1
Accession Version	XM_024984182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ATP synthase subunit alpha, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCG TGCGCGTCGC CGCGGCCGTG GCCCGCGCCC TCCCTCGGCG AGCGGGGCTG 
GTCTCCAAAA ATGCTTTGGG ATCATCCTTC ATTGCTGCAA GGAACCTGCA TGCCTCTAAC 
TCTCGTCTGC AGAAGACCGG CACTGCTGAG GTGTCCTCTA TTCTTGAAGA GCGTATTCTT 
GGAGCTGATA CCTCTGTTGA CCTTGAAGAG ACTGGGCGTG TTTTAAGTAT TGGTGATGGT 
ATTGCTCGGG TACATGGGCT AAGAAATGTT CAAGCAGAAG AAATGGTAGA GTTTTCTTCA 
GGTTTAAAGG GTATGTCTTT GAACTTGGAG CCTGACAATG TTGGTGTTGT CGTGTTTGGA 
AATGATAAAC TTATTAAGGA AGGAGATATT GTGAAGAGAA CTGGGGCTAT TGTAGATGTT 
CCAGTCGGCG AGGAGCTGCT GGGTCGTGTA GTTGATGCCC TTGGTAATGC CATTGATGGA 
AAGGGTCCAA TTGGTTCCAA GGCCCGAAGA CGAGTTGGCT TGAAAGCCCC TGGGATCATT 
CCTCGAATCT CTGTGAGGGA ACCAATGCAA ACTGGCATTA AGGCTGTGGA TAGCTTGGTG 
CCGATTGGTC GTGGTCAGCG TGAGCTGATT ATTGGTGACC GACAGACTGG CAAAACGTCA 
ATTGCAATTG ACACAATCAT TAACCAGAAA CGATTCAATG ATGGAACTGA TGAAAAGAAG 
AAGCTGTACT GTATCTATGT TGCTATTGGT CAAAAGAGAT CCACTGTTGC CCAGTTGGTG 
AAGAGACTTA CAGATGCAGA TGCCATGAAG TATACCATTG TGGTTTCAGC TACTGCTTCA 
GATGCTGCCC CACTTCAGTA CTTGGCTCCT TATTCTGGAT GTTCAATGGG AGAATATTTT 
AGGGATAATG GCAAACACGC TTTGATCATT TATGATGACT TATCCAAACA GGCGGTTGCT 
TATCGTCAGA TGTCTCTGCT GCTCCGCCGA CCCCCTGGTC GTGAGGCCTA TCCTGGTGAT 
GTATTCTACC TGCACTCCCG ACTCCTGGAG AGAGCAGCTA AAATGAACGA TGCTTTTGGT 
GGTGGCTCCT TGACTGCTCT ACCAGTCATC GAAACACAAG CTGGTGATGT GTCTGCTTAC 
ATTCCAACAA ATGTCATTTC CATCACTGAC GGACAGATCT TCTTGGAAAC AGAATTGTTC 
TACAAAGGTA TCCGCCCTGC CATTAATGTT GGTTTGTCTG TGTCCCGTGT CGGATCTGCT 
GCCCAAACCA GGGCCATGAA ACAGGTGGCA GGTACTATGA AACTGGAGTT GGCTCAGTAT 
CGTGAGGTTG CTGCTTTTGC CCAGTTCGGT TCTGACCTTG ATGCTGCCAC TCAACAACTC 
TTGAGTCGTG GTGTGCGTTT GACTGAGCTG CTCAAGCAGG GACAGTATTC TCCCATGGCT 
ATTGAAGAGC AAGTGGCTGT TATCTATGCG GGTGTCAGGG GGTATCTTGA CAAGCTGGAG 
CCCAGCAAGA TCACAAAATT TGAGAATGCT TTCTTGTCTC ACGTTATCAG CCAACATCAG 
GCCCTGTTGG GCAAAATCAG GACCGATGGA AAGATCTCAG AAGAGTCAGA TGCAAAGCTG 
AAAGAGATTG TAACAAACTT CTTGGCTGGA TTTGAAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839950.1
CDS	90..1751
Translation	MLSVRVAAAVARALPRRAGLVSKNALGSSFIAARNLHASNSRLQKTGTAEVSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDKLIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKARRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIGRGQRELIIGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAAPLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREVAAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVISQHQALLGKIRTDGKISEESDAKLKEIVTNFLAGFEA

Target ORF information:

RefSeq Version	XM_024984182.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP synthase F1 subunit alpha (ATP5F1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984182.1

ORF Insert Sequence:

ATGCTGTCCG TGCGCGTCGC CGCGGCCGTG GCCCGCGCCC TCCCTCGGCG AGCGGGGCTG 
GTCTCCAAAA ATGCTTTGGG ATCATCCTTC ATTGCTGCAA GGAACCTGCA TGCCTCTAAC 
TCTCGTCTGC AGAAGACCGG CACTGCTGAG GTGTCCTCTA TTCTTGAAGA GCGTATTCTT 
GGAGCTGATA CCTCTGTTGA CCTTGAAGAG ACTGGGCGTG TTTTAAGTAT TGGTGATGGT 
ATTGCTCGGG TACATGGGCT AAGAAATGTT CAAGCAGAAG AAATGGTAGA GTTTTCTTCA 
GGTTTAAAGG GTATGTCTTT GAACTTGGAG CCTGACAATG TTGGTGTTGT CGTGTTTGGA 
AATGATAAAC TTATTAAGGA AGGAGATATT GTGAAGAGAA CTGGGGCTAT TGTAGATGTT 
CCAGTCGGCG AGGAGCTGCT GGGTCGTGTA GTTGATGCCC TTGGTAATGC CATTGATGGA 
AAGGGTCCAA TTGGTTCCAA GGCCCGAAGA CGAGTTGGCT TGAAAGCCCC TGGGATCATT 
CCTCGAATCT CTGTGAGGGA ACCAATGCAA ACTGGCATTA AGGCTGTGGA TAGCTTGGTG 
CCGATTGGTC GTGGTCAGCG TGAGCTGATT ATTGGTGACC GACAGACTGG CAAAACGTCA 
ATTGCAATTG ACACAATCAT TAACCAGAAA CGATTCAATG ATGGAACTGA TGAAAAGAAG 
AAGCTGTACT GTATCTATGT TGCTATTGGT CAAAAGAGAT CCACTGTTGC CCAGTTGGTG 
AAGAGACTTA CAGATGCAGA TGCCATGAAG TATACCATTG TGGTTTCAGC TACTGCTTCA 
GATGCTGCCC CACTTCAGTA CTTGGCTCCT TATTCTGGAT GTTCAATGGG AGAATATTTT 
AGGGATAATG GCAAACACGC TTTGATCATT TATGATGACT TATCCAAACA GGCGGTTGCT 
TATCGTCAGA TGTCTCTGCT GCTCCGCCGA CCCCCTGGTC GTGAGGCCTA TCCTGGTGAT 
GTATTCTACC TGCACTCCCG ACTCCTGGAG AGAGCAGCTA AAATGAACGA TGCTTTTGGT 
GGTGGCTCCT TGACTGCTCT ACCAGTCATC GAAACACAAG CTGGTGATGT GTCTGCTTAC 
ATTCCAACAA ATGTCATTTC CATCACTGAC GGACAGATCT TCTTGGAAAC AGAATTGTTC 
TACAAAGGTA TCCGCCCTGC CATTAATGTT GGTTTGTCTG TGTCCCGTGT CGGATCTGCT 
GCCCAAACCA GGGCCATGAA ACAGGTGGCA GGTACTATGA AACTGGAGTT GGCTCAGTAT 
CGTGAGGTTG CTGCTTTTGC CCAGTTCGGT TCTGACCTTG ATGCTGCCAC TCAACAACTC 
TTGAGTCGTG GTGTGCGTTT GACTGAGCTG CTCAAGCAGG GACAGTATTC TCCCATGGCT 
ATTGAAGAGC AAGTGGCTGT TATCTATGCG GGTGTCAGGG GGTATCTTGA CAAGCTGGAG 
CCCAGCAAGA TCACAAAATT TGAGAATGCT TTCTTGTCTC ACGTTATCAG CCAACATCAG 
GCCCTGTTGG GCAAAATCAG GACCGATGGA AAGATCTCAG AAGAGTCAGA TGCAAAGCTG 
AAAGAGATTG TAACAAACTT CTTGGCTGGA TTTGAAGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM.
eLife4e10180(2015 Oct)
Zhou A,Rohou A,Schep DG,Bason JV,Montgomery MG,Walker JE,Grigorieff N,Rubinstein JL

Identification of the F1-ATPase at the cell surface of colonic epithelial cells: role in mediating cell proliferation.
The Journal of biological chemistry287(49)41458-68(2012 Nov)
Kowalski-Chauvel A,Najib S,Tikhonova IG,Huc L,Lopez F,Martinez LO,Cohen-Jonathan-Moyal E,Ferrand A,Seva C

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Mechanism of inhibition of bovine F1-ATPase by resveratrol and related polyphenols.
Proceedings of the National Academy of Sciences of the United States of America104(34)13632-7(2007 Aug)
Gledhill JR,Montgomery MG,Leslie AG,Walker JE

Association of two proteolipids of unknown function with ATP synthase from bovine heart mitochondria.
FEBS letters581(17)3145-8(2007 Jul)
Chen R,Runswick MJ,Carroll J,Fearnley IM,Walker JE

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ATP5F1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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