SLC24A1 cDNA ORF clone, Bos taurus(cattle)

The following SLC24A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC24A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219414 XM_010808871.3
Latest version!
Bos taurus solute carrier family 24 member 1 (SLC24A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa219414 XM_024997279.1
Latest version!
Bos taurus solute carrier family 24 member 1 (SLC24A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa41789 NM_174655.2
Latest version!
Bos taurus solute carrier family 24 member 1 (SLC24A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa22900 XM_010808871.2
Latest version!
Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa22901 XM_010808873.2
Latest version!
Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa45446 XM_010808867.2
Latest version!
Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa45448 XM_010808872.2
Latest version!
Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa45449 XM_010808874.2
Latest version!
Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa45450 XM_010808876.2
Latest version!
Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa45451 XM_010808877.2
Latest version!
Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa45452 XM_010808878.2
Latest version!
Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219414
    Clone ID Related Accession (Same CDS sequence) XM_010808871.3 , XM_024997279.1
    Accession Version XM_010808871.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010808871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGCTTT CATCCATGCT GACCCGAGGG AGCAGCTCGG CCTCTCTGCA CAACAGCACC
    ATCCGCAGCA CCATCTACCA GCTCATGCTC CACAGCCTGG ACCCTCTGGG GGAAGCCCGG
    CCCTCCAAGG ACAAGGAGGA GGAGACCTTG ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC
    AAAGCAGAGA GCAAACCAGA GGAGGAGCCG GCCAAGCTCC CTGAGGTCAC GGTCACACCA
    GCCCCTGCTC CAGACGTCAA GGGAGATCAG GAGGAGGATC CTGGCAGTCA GGGAGTCGGG
    GCCGAAGCTG AGAACACAGG TGAAAGGACA GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT
    GAAAACGGAG AGCGAAGTGG AGGCGACGCT GCATTAGGAG GTGAAAGTGA AGGAAAAGCA
    GAGAATGAAA GTGAAGGTGA CATCCCGGCA GAAAGGAGAG GAGATGATGA AGACGAAGGT
    GAAATCCAGG CAGAAGGTGG TGAAGTGAAA GGTGATGAAG ACGAAGGTGA AATCCAGGCA
    GGAGAAGGTG GGGAAGTGGA AGGTGATGAA GACGAAGGTG AAATCCAGGC AGGAGAAGGT
    GGGGAAGTGG AAGGTGATGA AGACGAAGGT GAAATCCAGG CAGGAGAAGG TGGGGAAGTG
    GAAGGTGATG AAGACGAAGG TGAAATCCAG GCAGGAGAAG GTGGGGAAGT GGAAGGTGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GCTGGGGAAG TGGAAGGTGA TGAAGACGAA
    GGTGAAATCC AGGCAGGAGA AGGTGGTGAA GTGAAAGGTG ATGAAGGTGA AATCCAGGCA
    GGAGAAGCTG GGGAAGTGGA AGGAGAGGAT GGTGAAGTGG AAGGTGGTGA AGATGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGTGAAGGT GAAACTGGAG AGCAGGAATT GAATGCTGAA
    ATTCAAGGTG AAGCCAAAGA TGACGAGGAA GGTGTGGATG GTGAAGGGGG AGGTGATGGA
    GGTGACAGTG AAGATGAAGA AGAGGAAGAT GAAGAAGAGG AAGACGAAGA AGAGGAAGAG
    GAGGAAGAGG AGGAGGAAGA GGAAAATGAA CAGCCTTTGT CCCTGGAATG GCCTGAGACC
    AGGCGGAAGC AAGCCATTTA CCTCTTTCTC CTGCCCATTG TGTTCCCGCT GTGGCTGACG
    GTTCCCGACG TCCGGAGGCT GGAGGCTAAG AAGTTTTTTG TTATCACCTT CCTGGGATCC
    ATCCTGTGGA TAGCCATGTT CTCATACCTC ATGGTTTGGT GGGCTCACCA GGTTGGCGAA
    ACAATAGGGA TTTCTGAAGA GATCATGGGT TTGACGATCC TAGCAGCAGG CACATCAATT
    CCTGACCTCA TCACCAGTGT GATTGTCGCT CGGAAAGGCC TGGGTGACAT GGCTGTGTCA
    AGCTCCGTGG GCAGTAACAT ATTTGATATC ACCGTGGGCC TGCCCCTTCC TTGGATGCTT
    TTCTCTCTCA TCAATGGATT GCAGCCTGTT GCTGTCAGTA GCAATGGCTT GTTCTGTGCA
    ATTGTTTTGC TTTTTCTCAT GCTCCTGTTT GTGATCTCGT CAATTGCATT ATGCAAGTGG
    AGAATGAACA AGATCCTGGG CTTCACCATG TTCCTCCTGT ACTTTGTATT CCTGATAATC
    AGTGTGATGC TGGAAGATCG AATCATATCC TGCCCTGTAT CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807173.2
    CDS206..3853
    Translation

    Target ORF information:

    RefSeq Version XM_010808871.3
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 member 1 (SLC24A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808871.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGCTTT CATCCATGCT GACCCGAGGG AGCAGCTCGG CCTCTCTGCA CAACAGCACC
    ATCCGCAGCA CCATCTACCA GCTCATGCTC CACAGCCTGG ACCCTCTGGG GGAAGCCCGG
    CCCTCCAAGG ACAAGGAGGA GGAGACCTTG ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC
    AAAGCAGAGA GCAAACCAGA GGAGGAGCCG GCCAAGCTCC CTGAGGTCAC GGTCACACCA
    GCCCCTGCTC CAGACGTCAA GGGAGATCAG GAGGAGGATC CTGGCAGTCA GGGAGTCGGG
    GCCGAAGCTG AGAACACAGG TGAAAGGACA GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT
    GAAAACGGAG AGCGAAGTGG AGGCGACGCT GCATTAGGAG GTGAAAGTGA AGGAAAAGCA
    GAGAATGAAA GTGAAGGTGA CATCCCGGCA GAAAGGAGAG GAGATGATGA AGACGAAGGT
    GAAATCCAGG CAGAAGGTGG TGAAGTGAAA GGTGATGAAG ACGAAGGTGA AATCCAGGCA
    GGAGAAGGTG GGGAAGTGGA AGGTGATGAA GACGAAGGTG AAATCCAGGC AGGAGAAGGT
    GGGGAAGTGG AAGGTGATGA AGACGAAGGT GAAATCCAGG CAGGAGAAGG TGGGGAAGTG
    GAAGGTGATG AAGACGAAGG TGAAATCCAG GCAGGAGAAG GTGGGGAAGT GGAAGGTGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GCTGGGGAAG TGGAAGGTGA TGAAGACGAA
    GGTGAAATCC AGGCAGGAGA AGGTGGTGAA GTGAAAGGTG ATGAAGGTGA AATCCAGGCA
    GGAGAAGCTG GGGAAGTGGA AGGAGAGGAT GGTGAAGTGG AAGGTGGTGA AGATGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGTGAAGGT GAAACTGGAG AGCAGGAATT GAATGCTGAA
    ATTCAAGGTG AAGCCAAAGA TGACGAGGAA GGTGTGGATG GTGAAGGGGG AGGTGATGGA
    GGTGACAGTG AAGATGAAGA AGAGGAAGAT GAAGAAGAGG AAGACGAAGA AGAGGAAGAG
    GAGGAAGAGG AGGAGGAAGA GGAAAATGAA CAGCCTTTGT CCCTGGAATG GCCTGAGACC
    AGGCGGAAGC AAGCCATTTA CCTCTTTCTC CTGCCCATTG TGTTCCCGCT GTGGCTGACG
    GTTCCCGACG TCCGGAGGCT GGAGGCTAAG AAGTTTTTTG TTATCACCTT CCTGGGATCC
    ATCCTGTGGA TAGCCATGTT CTCATACCTC ATGGTTTGGT GGGCTCACCA GGTTGGCGAA
    ACAATAGGGA TTTCTGAAGA GATCATGGGT TTGACGATCC TAGCAGCAGG CACATCAATT
    CCTGACCTCA TCACCAGTGT GATTGTCGCT CGGAAAGGCC TGGGTGACAT GGCTGTGTCA
    AGCTCCGTGG GCAGTAACAT ATTTGATATC ACCGTGGGCC TGCCCCTTCC TTGGATGCTT
    TTCTCTCTCA TCAATGGATT GCAGCCTGTT GCTGTCAGTA GCAATGGCTT GTTCTGTGCA
    ATTGTTTTGC TTTTTCTCAT GCTCCTGTTT GTGATCTCGT CAATTGCATT ATGCAAGTGG
    AGAATGAACA AGATCCTGGG CTTCACCATG TTCCTCCTGT ACTTTGTATT CCTGATAATC
    AGTGTGATGC TGGAAGATCG AATCATATCC TGCCCTGTAT CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219414
    Clone ID Related Accession (Same CDS sequence) XM_010808871.3 , XM_024997279.1
    Accession Version XM_024997279.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGCTTT CATCCATGCT GACCCGAGGG AGCAGCTCGG CCTCTCTGCA CAACAGCACC
    ATCCGCAGCA CCATCTACCA GCTCATGCTC CACAGCCTGG ACCCTCTGGG GGAAGCCCGG
    CCCTCCAAGG ACAAGGAGGA GGAGACCTTG ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC
    AAAGCAGAGA GCAAACCAGA GGAGGAGCCG GCCAAGCTCC CTGAGGTCAC GGTCACACCA
    GCCCCTGCTC CAGACGTCAA GGGAGATCAG GAGGAGGATC CTGGCAGTCA GGGAGTCGGG
    GCCGAAGCTG AGAACACAGG TGAAAGGACA GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT
    GAAAACGGAG AGCGAAGTGG AGGCGACGCT GCATTAGGAG GTGAAAGTGA AGGAAAAGCA
    GAGAATGAAA GTGAAGGTGA CATCCCGGCA GAAAGGAGAG GAGATGATGA AGACGAAGGT
    GAAATCCAGG CAGAAGGTGG TGAAGTGAAA GGTGATGAAG ACGAAGGTGA AATCCAGGCA
    GGAGAAGGTG GGGAAGTGGA AGGTGATGAA GACGAAGGTG AAATCCAGGC AGGAGAAGGT
    GGGGAAGTGG AAGGTGATGA AGACGAAGGT GAAATCCAGG CAGGAGAAGG TGGGGAAGTG
    GAAGGTGATG AAGACGAAGG TGAAATCCAG GCAGGAGAAG GTGGGGAAGT GGAAGGTGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GCTGGGGAAG TGGAAGGTGA TGAAGACGAA
    GGTGAAATCC AGGCAGGAGA AGGTGGTGAA GTGAAAGGTG ATGAAGGTGA AATCCAGGCA
    GGAGAAGCTG GGGAAGTGGA AGGAGAGGAT GGTGAAGTGG AAGGTGGTGA AGATGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGTGAAGGT GAAACTGGAG AGCAGGAATT GAATGCTGAA
    ATTCAAGGTG AAGCCAAAGA TGACGAGGAA GGTGTGGATG GTGAAGGGGG AGGTGATGGA
    GGTGACAGTG AAGATGAAGA AGAGGAAGAT GAAGAAGAGG AAGACGAAGA AGAGGAAGAG
    GAGGAAGAGG AGGAGGAAGA GGAAAATGAA CAGCCTTTGT CCCTGGAATG GCCTGAGACC
    AGGCGGAAGC AAGCCATTTA CCTCTTTCTC CTGCCCATTG TGTTCCCGCT GTGGCTGACG
    GTTCCCGACG TCCGGAGGCT GGAGGCTAAG AAGTTTTTTG TTATCACCTT CCTGGGATCC
    ATCCTGTGGA TAGCCATGTT CTCATACCTC ATGGTTTGGT GGGCTCACCA GGTTGGCGAA
    ACAATAGGGA TTTCTGAAGA GATCATGGGT TTGACGATCC TAGCAGCAGG CACATCAATT
    CCTGACCTCA TCACCAGTGT GATTGTCGCT CGGAAAGGCC TGGGTGACAT GGCTGTGTCA
    AGCTCCGTGG GCAGTAACAT ATTTGATATC ACCGTGGGCC TGCCCCTTCC TTGGATGCTT
    TTCTCTCTCA TCAATGGATT GCAGCCTGTT GCTGTCAGTA GCAATGGCTT GTTCTGTGCA
    ATTGTTTTGC TTTTTCTCAT GCTCCTGTTT GTGATCTCGT CAATTGCATT ATGCAAGTGG
    AGAATGAACA AGATCCTGGG CTTCACCATG TTCCTCCTGT ACTTTGTATT CCTGATAATC
    AGTGTGATGC TGGAAGATCG AATCATATCC TGCCCTGTAT CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853047.1
    CDS164..3811
    Translation

    Target ORF information:

    RefSeq Version XM_024997279.1
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 member 1 (SLC24A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997279.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGCTTT CATCCATGCT GACCCGAGGG AGCAGCTCGG CCTCTCTGCA CAACAGCACC
    ATCCGCAGCA CCATCTACCA GCTCATGCTC CACAGCCTGG ACCCTCTGGG GGAAGCCCGG
    CCCTCCAAGG ACAAGGAGGA GGAGACCTTG ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC
    AAAGCAGAGA GCAAACCAGA GGAGGAGCCG GCCAAGCTCC CTGAGGTCAC GGTCACACCA
    GCCCCTGCTC CAGACGTCAA GGGAGATCAG GAGGAGGATC CTGGCAGTCA GGGAGTCGGG
    GCCGAAGCTG AGAACACAGG TGAAAGGACA GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT
    GAAAACGGAG AGCGAAGTGG AGGCGACGCT GCATTAGGAG GTGAAAGTGA AGGAAAAGCA
    GAGAATGAAA GTGAAGGTGA CATCCCGGCA GAAAGGAGAG GAGATGATGA AGACGAAGGT
    GAAATCCAGG CAGAAGGTGG TGAAGTGAAA GGTGATGAAG ACGAAGGTGA AATCCAGGCA
    GGAGAAGGTG GGGAAGTGGA AGGTGATGAA GACGAAGGTG AAATCCAGGC AGGAGAAGGT
    GGGGAAGTGG AAGGTGATGA AGACGAAGGT GAAATCCAGG CAGGAGAAGG TGGGGAAGTG
    GAAGGTGATG AAGACGAAGG TGAAATCCAG GCAGGAGAAG GTGGGGAAGT GGAAGGTGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GCTGGGGAAG TGGAAGGTGA TGAAGACGAA
    GGTGAAATCC AGGCAGGAGA AGGTGGTGAA GTGAAAGGTG ATGAAGGTGA AATCCAGGCA
    GGAGAAGCTG GGGAAGTGGA AGGAGAGGAT GGTGAAGTGG AAGGTGGTGA AGATGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGTGAAGGT GAAACTGGAG AGCAGGAATT GAATGCTGAA
    ATTCAAGGTG AAGCCAAAGA TGACGAGGAA GGTGTGGATG GTGAAGGGGG AGGTGATGGA
    GGTGACAGTG AAGATGAAGA AGAGGAAGAT GAAGAAGAGG AAGACGAAGA AGAGGAAGAG
    GAGGAAGAGG AGGAGGAAGA GGAAAATGAA CAGCCTTTGT CCCTGGAATG GCCTGAGACC
    AGGCGGAAGC AAGCCATTTA CCTCTTTCTC CTGCCCATTG TGTTCCCGCT GTGGCTGACG
    GTTCCCGACG TCCGGAGGCT GGAGGCTAAG AAGTTTTTTG TTATCACCTT CCTGGGATCC
    ATCCTGTGGA TAGCCATGTT CTCATACCTC ATGGTTTGGT GGGCTCACCA GGTTGGCGAA
    ACAATAGGGA TTTCTGAAGA GATCATGGGT TTGACGATCC TAGCAGCAGG CACATCAATT
    CCTGACCTCA TCACCAGTGT GATTGTCGCT CGGAAAGGCC TGGGTGACAT GGCTGTGTCA
    AGCTCCGTGG GCAGTAACAT ATTTGATATC ACCGTGGGCC TGCCCCTTCC TTGGATGCTT
    TTCTCTCTCA TCAATGGATT GCAGCCTGTT GCTGTCAGTA GCAATGGCTT GTTCTGTGCA
    ATTGTTTTGC TTTTTCTCAT GCTCCTGTTT GTGATCTCGT CAATTGCATT ATGCAAGTGG
    AGAATGAACA AGATCCTGGG CTTCACCATG TTCCTCCTGT ACTTTGTATT CCTGATAATC
    AGTGTGATGC TGGAAGATCG AATCATATCC TGCCCTGTAT CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41789
    Clone ID Related Accession (Same CDS sequence) NM_174655.2
    Accession Version NM_174655.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X66481.1. On Jun 6, 2003 this sequence version replaced NM_174655.1. ##Evidence-Data-START## Transcript exon combination :: X66481.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCGTG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGCTTT CATCCATGCT GACCCGAGGG AGCAGCTCGG CCTCTCTGCA CAACAGCACC
    ATCCGCAGCA CCATCTACCA GCTCATGCTC CACAGCCTGG ACCCTCTGGG GGAAGCCCGG
    CCCTCCAAGG ACAAGGAGGA GGAGACCTTG ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC
    AAAGCAGAGA GCAAACCAGA GGAGGAGCCG GCCAAGCTCC CTGAGGTCAC GGTCACACCA
    GCCCCTGCTC CAGACGTCAA GGGAGATCAG GAGGAGGATC CTGGCAGTCA GGGAGTCGGG
    GCCGAAGCTG AGAACACAGG TGAAAGGACA GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT
    GAAAACGGAG AGCGAAGTGG AGGCGACGCT GCATTAGGAG GTGAAAGTGA AGGAAAAGCA
    GAGAATGAAA GTGAAGGTGA CATCCCGGCA GAAAGGAGAG GAGATGATGA AGACGAAGGT
    GAAATCCAGG CAGAAGGTGG TGAAGTGAAA GGTGATGAAG ACGAAGGTGA AATCCAGGCA
    GGAGAAGGTG GGGAAGTGGA AGGTGATGAA GACGAAGGTG AAATCCAGGC AGGAGAAGGT
    GGGGAAGTGG AAGGTGATGA AGACGAAGGT GAAATCCAGG CAGGAGAAGC TGGGGAAGTG
    GAAGGTGATG AAGACGAAGG TGAAATCCAG GCAGGAGAAG CTGGGGAAGT GGAAGGTGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GGTGGTGAAG TGAAAGGTGA TGAAGGTGAA
    ATCCAGGCAG GAGAAGCTGG GGAAGTGGAA GGAGAGGATG GTGAAGTGGA AGGTGGTGAA
    GATGAAGGTG AAATCCAGGC AGGAGAAGGT GGTGAAGGTG AAACTGGAGA GCAGGAATTG
    AATGCTGAAA TTCAAGGTGA AGCCAAAGAT GACGAGGAAG GTGTGGATGG TGAAGGGGGA
    GGTGATGGAG GTGACAGTGA AGATGAAGAA GAGGAAGATG AAGAAGAGGA AGACGAAGAA
    GAGGAAGAGG AGGAAGAGGA GGAGGAAGAG GAGGAAAATG AACAGCCTTT GTCCCTGGAA
    TGGCCTGAGA CCAGGCGGAA GCAAGCCATT TACCTCTTTC TCCTGCCCAT TGTGTTCCCG
    CTGTGGCTGA CGGTTCCCGA CGTCCGGAGG CTGGAGGCTA AGAAGTTTTT TGTTATCACC
    TTCCTGGGAT CCATCCTGTG GATAGCCATG TTCTCATACC TCATGGTTTG GTGGGCTCAC
    CAGGTTGGCG AAACAATAGG GATTTCTGAA GAGATCATGG GTTTGACGAT CCTAGCAGCA
    GGCACATCAA TTCCTGACCT CATCACCAGT GTGATTGTCG CTCGGAAAGG CCTGGGTGAC
    ATGGCTGTGT CAAGCTCCGT GGGCAGTAAC ATATTTGATA TCACCGTGGG CCTGCCCCTT
    CCTTGGATGC TTTTCTCTCT CATCAATGGA TTGCAGCCTG TTGCTGTCAG TAGCAATGGC
    TTGTTCTGTG CAATTGTTTT GCTTTTTCTC ATGCTCCTGT TTGTGATCTC GTCAATTGCA
    TTATGCAAGT GGAGAATGAA CAAGATCCTG GGCTTCACCA TGTTCCTCCT GTACTTTGTA
    TTCCTGATAA TCAGTGTGAT GCTGGAAGAT CGAATCATAT CCTGCCCTGT ATCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_777080.1
    CDS212..3811
    Translation

    Target ORF information:

    RefSeq Version NM_174655.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 member 1 (SLC24A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174655.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCGTG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGCTTT CATCCATGCT GACCCGAGGG AGCAGCTCGG CCTCTCTGCA CAACAGCACC
    ATCCGCAGCA CCATCTACCA GCTCATGCTC CACAGCCTGG ACCCTCTGGG GGAAGCCCGG
    CCCTCCAAGG ACAAGGAGGA GGAGACCTTG ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC
    AAAGCAGAGA GCAAACCAGA GGAGGAGCCG GCCAAGCTCC CTGAGGTCAC GGTCACACCA
    GCCCCTGCTC CAGACGTCAA GGGAGATCAG GAGGAGGATC CTGGCAGTCA GGGAGTCGGG
    GCCGAAGCTG AGAACACAGG TGAAAGGACA GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT
    GAAAACGGAG AGCGAAGTGG AGGCGACGCT GCATTAGGAG GTGAAAGTGA AGGAAAAGCA
    GAGAATGAAA GTGAAGGTGA CATCCCGGCA GAAAGGAGAG GAGATGATGA AGACGAAGGT
    GAAATCCAGG CAGAAGGTGG TGAAGTGAAA GGTGATGAAG ACGAAGGTGA AATCCAGGCA
    GGAGAAGGTG GGGAAGTGGA AGGTGATGAA GACGAAGGTG AAATCCAGGC AGGAGAAGGT
    GGGGAAGTGG AAGGTGATGA AGACGAAGGT GAAATCCAGG CAGGAGAAGC TGGGGAAGTG
    GAAGGTGATG AAGACGAAGG TGAAATCCAG GCAGGAGAAG CTGGGGAAGT GGAAGGTGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GGTGGTGAAG TGAAAGGTGA TGAAGGTGAA
    ATCCAGGCAG GAGAAGCTGG GGAAGTGGAA GGAGAGGATG GTGAAGTGGA AGGTGGTGAA
    GATGAAGGTG AAATCCAGGC AGGAGAAGGT GGTGAAGGTG AAACTGGAGA GCAGGAATTG
    AATGCTGAAA TTCAAGGTGA AGCCAAAGAT GACGAGGAAG GTGTGGATGG TGAAGGGGGA
    GGTGATGGAG GTGACAGTGA AGATGAAGAA GAGGAAGATG AAGAAGAGGA AGACGAAGAA
    GAGGAAGAGG AGGAAGAGGA GGAGGAAGAG GAGGAAAATG AACAGCCTTT GTCCCTGGAA
    TGGCCTGAGA CCAGGCGGAA GCAAGCCATT TACCTCTTTC TCCTGCCCAT TGTGTTCCCG
    CTGTGGCTGA CGGTTCCCGA CGTCCGGAGG CTGGAGGCTA AGAAGTTTTT TGTTATCACC
    TTCCTGGGAT CCATCCTGTG GATAGCCATG TTCTCATACC TCATGGTTTG GTGGGCTCAC
    CAGGTTGGCG AAACAATAGG GATTTCTGAA GAGATCATGG GTTTGACGAT CCTAGCAGCA
    GGCACATCAA TTCCTGACCT CATCACCAGT GTGATTGTCG CTCGGAAAGG CCTGGGTGAC
    ATGGCTGTGT CAAGCTCCGT GGGCAGTAAC ATATTTGATA TCACCGTGGG CCTGCCCCTT
    CCTTGGATGC TTTTCTCTCT CATCAATGGA TTGCAGCCTG TTGCTGTCAG TAGCAATGGC
    TTGTTCTGTG CAATTGTTTT GCTTTTTCTC ATGCTCCTGT TTGTGATCTC GTCAATTGCA
    TTATGCAAGT GGAGAATGAA CAAGATCCTG GGCTTCACCA TGTTCCTCCT GTACTTTGTA
    TTCCTGATAA TCAGTGTGAT GCTGGAAGAT CGAATCATAT CCTGCCCTGT ATCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22900
    Clone ID Related Accession (Same CDS sequence) XM_010808871.2
    Accession Version XM_010808871.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3651bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGCTTT CATCCATGCT GACCCGAGGG AGCAGCTCGG CCTCTCTGCA CAACAGCACC
    ATCCGCAGCA CCATCTACCA GCTCATGCTC CACAGCCTGG ACCCTCTGGG GGAAGCCCGG
    CCCTCCAAGG ACAAGGAGGA GGAGACCTTG ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC
    AAAGCAGAGA GCAAACCAGA GGAGGAGCCG GCCAAGCTCC CTGAGGTCAC GGTCACACCA
    GCCCCTGCTC CAGACGTCAA GGGAGATCAG GAGGAGGATC CTGGCAGTCA GGGAGTCGGG
    GCCGAAGCTG AGAACACAGG TGAAAGGACA GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT
    GAAAACGGAG AGCGAAGTGG AGGCGACGCT GCATTAGGAG GTGAAAGTGA AGGAAAAGCA
    GAGAATGAAA GTGAAGGTGA CATCCCGGCA GAAAGGAGAG GAGATGATGA AGACGAAGGT
    GAAATCCAGG CAGAAGGTGG TGAAGTGAAA GGTGATGAAG ACGAAGGTGA AATCCAGGCA
    GGAGAAGGTG GGGAAGTGGA AGGTGATGAA GACGAAGGTG AAATCCAGGC AGGAGAAGGT
    GGGGAAGTGG AAGGTGATGA AGACGAAGGT GAAATCCAGG CAGGAGAAGG TGGGGAAGTG
    GAAGGTGATG AAGACGAAGG TGAAATCCAG GCAGGAGAAG CTGGGGAAGT GGAAGGTGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GCTGGGGAAG TGGAAGGTGA TGAAGACGAA
    GGTGAAATCC AGGCAGGAGA AGGTGGTGAA GTGAAAGGTG ATGAAGGTGA AATCCAGGCA
    GGAGAAGCTG GGGAAGTGGA AGGAGAGGAT GGTGAAGTGG AAGGTGGTGA AGATGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGTGAAGGT GAAACTGGAG AGCAGGAATT GAATGCTGAA
    ATTCAAGGTG AAGCCAAAGA TGACGAGGAA GGTGTGGATG GTGAAGGGGG AGGTGATGGA
    GGTGACAGTG AAGATGAAGA AGAGGAAGAT GAAGAAGAGG AAGACGAAGA AGAGGAAGAG
    GAGGAAGAGG AGGAGGAAGA GGAGGAAAAT GAACAGCCTT TGTCCCTGGA ATGGCCTGAG
    ACCAGGCGGA AGCAAGCCAT TTACCTCTTT CTCCTGCCCA TTGTGTTCCC GCTGTGGCTG
    ACGGTTCCCG ACGTCCGGAG GCTGGAGGCT AAGAAGTTTT TTGTTATCAC CTTCCTGGGA
    TCCATCCTGT GGATAGCCAT GTTCTCATAC CTCATGGTTT GGTGGGCTCA CCAGGTTGGC
    GAAACAATAG GGATTTCTGA AGAGATCATG GGTTTGACGA TCCTAGCAGC AGGCACATCA
    ATTCCTGACC TCATCACCAG TGTGATTGTC GCTCGGAAAG GCCTGGGTGA CATGGCTGTG
    TCAAGCTCCG TGGGCAGTAA CATATTTGAT ATCACCGTGG GCCTGCCCCT TCCTTGGATG
    CTTTTCTCTC TCATCAATGG ATTGCAGCCT GTTGCTGTCA GTAGCAATGG CTTGTTCTGT
    GCAATTGTTT TGCTTTTTCT CATGCTCCTG TTTGTGATCT CGTCAATTGC ATTATGCAAG
    TGGAGAATGA ACAAGATCCT GGGCTTCACC ATGTTCCTCC TGTACTTTGT ATTCCTGATA
    ATCAGTGTGA TGCTGGAAGA TCGAATCATA TCCTGCCCTG TATCTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807173.1
    CDS280..3930
    Translation

    Target ORF information:

    RefSeq Version XM_010808871.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808871.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGCTTT CATCCATGCT GACCCGAGGG AGCAGCTCGG CCTCTCTGCA CAACAGCACC
    ATCCGCAGCA CCATCTACCA GCTCATGCTC CACAGCCTGG ACCCTCTGGG GGAAGCCCGG
    CCCTCCAAGG ACAAGGAGGA GGAGACCTTG ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC
    AAAGCAGAGA GCAAACCAGA GGAGGAGCCG GCCAAGCTCC CTGAGGTCAC GGTCACACCA
    GCCCCTGCTC CAGACGTCAA GGGAGATCAG GAGGAGGATC CTGGCAGTCA GGGAGTCGGG
    GCCGAAGCTG AGAACACAGG TGAAAGGACA GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT
    GAAAACGGAG AGCGAAGTGG AGGCGACGCT GCATTAGGAG GTGAAAGTGA AGGAAAAGCA
    GAGAATGAAA GTGAAGGTGA CATCCCGGCA GAAAGGAGAG GAGATGATGA AGACGAAGGT
    GAAATCCAGG CAGAAGGTGG TGAAGTGAAA GGTGATGAAG ACGAAGGTGA AATCCAGGCA
    GGAGAAGGTG GGGAAGTGGA AGGTGATGAA GACGAAGGTG AAATCCAGGC AGGAGAAGGT
    GGGGAAGTGG AAGGTGATGA AGACGAAGGT GAAATCCAGG CAGGAGAAGG TGGGGAAGTG
    GAAGGTGATG AAGACGAAGG TGAAATCCAG GCAGGAGAAG CTGGGGAAGT GGAAGGTGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GCTGGGGAAG TGGAAGGTGA TGAAGACGAA
    GGTGAAATCC AGGCAGGAGA AGGTGGTGAA GTGAAAGGTG ATGAAGGTGA AATCCAGGCA
    GGAGAAGCTG GGGAAGTGGA AGGAGAGGAT GGTGAAGTGG AAGGTGGTGA AGATGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGTGAAGGT GAAACTGGAG AGCAGGAATT GAATGCTGAA
    ATTCAAGGTG AAGCCAAAGA TGACGAGGAA GGTGTGGATG GTGAAGGGGG AGGTGATGGA
    GGTGACAGTG AAGATGAAGA AGAGGAAGAT GAAGAAGAGG AAGACGAAGA AGAGGAAGAG
    GAGGAAGAGG AGGAGGAAGA GGAGGAAAAT GAACAGCCTT TGTCCCTGGA ATGGCCTGAG
    ACCAGGCGGA AGCAAGCCAT TTACCTCTTT CTCCTGCCCA TTGTGTTCCC GCTGTGGCTG
    ACGGTTCCCG ACGTCCGGAG GCTGGAGGCT AAGAAGTTTT TTGTTATCAC CTTCCTGGGA
    TCCATCCTGT GGATAGCCAT GTTCTCATAC CTCATGGTTT GGTGGGCTCA CCAGGTTGGC
    GAAACAATAG GGATTTCTGA AGAGATCATG GGTTTGACGA TCCTAGCAGC AGGCACATCA
    ATTCCTGACC TCATCACCAG TGTGATTGTC GCTCGGAAAG GCCTGGGTGA CATGGCTGTG
    TCAAGCTCCG TGGGCAGTAA CATATTTGAT ATCACCGTGG GCCTGCCCCT TCCTTGGATG
    CTTTTCTCTC TCATCAATGG ATTGCAGCCT GTTGCTGTCA GTAGCAATGG CTTGTTCTGT
    GCAATTGTTT TGCTTTTTCT CATGCTCCTG TTTGTGATCT CGTCAATTGC ATTATGCAAG
    TGGAGAATGA ACAAGATCCT GGGCTTCACC ATGTTCCTCC TGTACTTTGT ATTCCTGATA
    ATCAGTGTGA TGCTGGAAGA TCGAATCATA TCCTGCCCTG TATCTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22901
    Clone ID Related Accession (Same CDS sequence) XM_010808873.2
    Accession Version XM_010808873.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808873.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCTTTCATC CATGCTGACC CGAGGGAGCA GCTCGGCCTC TCTGCACAAC
    AGCACCATCC GCAGCACCAT CTACCAGCTC ATGCTCCACA GCCTGGACCC TCTGGGGGAA
    GCCCGGCCCT CCAAGGACAA GGAGGAGGAG ACCTTGATTC CGGAGGCAAA AGCCACGCCT
    CAGGCCAAAG CAGAGAGCAA ACCAGAGGAG GAGCCGGCCA AGCTCCCTGA GGTCACGGTC
    ACACCAGCCC CTGCTCCAGA CGTCAAGGGA GATCAGGAGG AGGATCCTGG CAGTCAGGGA
    GTCGGGGCCG AAGCTGAGAA CACAGGTGAA AGGACAGGTG GAGAGGCGGA AGCTCCTGCT
    GAAGGTGAAA ACGGAGAGCG AAGTGGAGGC GACGCTGCAT TAGGAGGTGA AAGTGAAGGA
    AAAGCAGAGA ATGAAAGTGA AGGTGACATC CCGGCAGAAA GGAGAGGAGA TGATGAAGAC
    GAAGGTGAAA TCCAGGCAGA AGGTGGTGAA GTGAAAGGTG ATGAAGACGA AGGTGAAATC
    CAGGCAGGAG AAGGTGGGGA AGTGGAAGGT GATGAAGACG AAGGTGAAAT CCAGGCAGGA
    GAAGGTGGGG AAGTGGAAGG TGATGAAGAC GAAGGTGAAA TCCAGGCAGG AGAAGGTGGG
    GAAGTGGAAG GTGATGAAGA CGAAGGTGAA ATCCAGGCAG GAGAAGCTGG GGAAGTGGAA
    GGTGATGAAG ACGAAGGTGA AATCCAGGCA GGAGAAGCTG GGGAAGTGGA AGGTGATGAA
    GACGAAGGTG AAATCCAGGC AGGAGAAGGT GGTGAAGTGA AAGGTGATGA AGGTGAAATC
    CAGGCAGGAG AAGCTGGGGA AGTGGAAGGA GAGGATGGTG AAGTGGAAGG TGGTGAAGAT
    GAAGGTGAAA TCCAGGCAGG AGAAGGTGGT GAAGGTGAAA CTGGAGAGCA GGAATTGAAT
    GCTGAAATTC AAGGTGAAGC CAAAGATGAC GAGGAAGGTG TGGATGGTGA AGGGGGAGGT
    GATGGAGGTG ACAGTGAAGA TGAAGAAGAG GAAGATGAAG AAGAGGAAGA CGAAGAAGAG
    GAAGAGGAGG AAGAGGAGGA GGAAGAGGAG GAAAATGAAC AGCCTTTGTC CCTGGAATGG
    CCTGAGACCA GGCGGAAGCA AGCCATTTAC CTCTTTCTCC TGCCCATTGT GTTCCCGCTG
    TGGCTGACGG TTCCCGACGT CCGGAGGCTG GAGGCTAAGA AGTTTTTTGT TATCACCTTC
    CTGGGATCCA TCCTGTGGAT AGCCATGTTC TCATACCTCA TGGTTTGGTG GGCTCACCAG
    GTTGGCGAAA CAATAGGGAT TTCTGAAGAG ATCATGGGTT TGACGATCCT AGCAGCAGGC
    ACATCAATTC CTGACCTCAT CACCAGTGTG ATTGTCGCTC GGAAAGGCCT GGGTGACATG
    GCTGTGTCAA GCTCCGTGGG CAGTAACATA TTTGATATCA CCGTGGGCCT GCCCCTTCCT
    TGGATGCTTT TCTCTCTCAT CAATGGATTG CAGCCTGTTG CTGTCAGTAG CAATGGCTTG
    TTCTGTGCAA TTGTTTTGCT TTTTCTCATG CTCCTGTTTG TGATCTCGTC AATTGCATTA
    TGCAAGTGGA GAATGAACAA GATCCTGGGC TTCACCATGT TCCTCCTGTA CTTTGTATTC
    CTGATAATCA GTGTGATGCT GGAAGATCGA ATCATATCCT GCCCTGTATC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807175.1
    CDS280..3876
    Translation

    Target ORF information:

    RefSeq Version XM_010808873.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808873.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCTTTCATC CATGCTGACC CGAGGGAGCA GCTCGGCCTC TCTGCACAAC
    AGCACCATCC GCAGCACCAT CTACCAGCTC ATGCTCCACA GCCTGGACCC TCTGGGGGAA
    GCCCGGCCCT CCAAGGACAA GGAGGAGGAG ACCTTGATTC CGGAGGCAAA AGCCACGCCT
    CAGGCCAAAG CAGAGAGCAA ACCAGAGGAG GAGCCGGCCA AGCTCCCTGA GGTCACGGTC
    ACACCAGCCC CTGCTCCAGA CGTCAAGGGA GATCAGGAGG AGGATCCTGG CAGTCAGGGA
    GTCGGGGCCG AAGCTGAGAA CACAGGTGAA AGGACAGGTG GAGAGGCGGA AGCTCCTGCT
    GAAGGTGAAA ACGGAGAGCG AAGTGGAGGC GACGCTGCAT TAGGAGGTGA AAGTGAAGGA
    AAAGCAGAGA ATGAAAGTGA AGGTGACATC CCGGCAGAAA GGAGAGGAGA TGATGAAGAC
    GAAGGTGAAA TCCAGGCAGA AGGTGGTGAA GTGAAAGGTG ATGAAGACGA AGGTGAAATC
    CAGGCAGGAG AAGGTGGGGA AGTGGAAGGT GATGAAGACG AAGGTGAAAT CCAGGCAGGA
    GAAGGTGGGG AAGTGGAAGG TGATGAAGAC GAAGGTGAAA TCCAGGCAGG AGAAGGTGGG
    GAAGTGGAAG GTGATGAAGA CGAAGGTGAA ATCCAGGCAG GAGAAGCTGG GGAAGTGGAA
    GGTGATGAAG ACGAAGGTGA AATCCAGGCA GGAGAAGCTG GGGAAGTGGA AGGTGATGAA
    GACGAAGGTG AAATCCAGGC AGGAGAAGGT GGTGAAGTGA AAGGTGATGA AGGTGAAATC
    CAGGCAGGAG AAGCTGGGGA AGTGGAAGGA GAGGATGGTG AAGTGGAAGG TGGTGAAGAT
    GAAGGTGAAA TCCAGGCAGG AGAAGGTGGT GAAGGTGAAA CTGGAGAGCA GGAATTGAAT
    GCTGAAATTC AAGGTGAAGC CAAAGATGAC GAGGAAGGTG TGGATGGTGA AGGGGGAGGT
    GATGGAGGTG ACAGTGAAGA TGAAGAAGAG GAAGATGAAG AAGAGGAAGA CGAAGAAGAG
    GAAGAGGAGG AAGAGGAGGA GGAAGAGGAG GAAAATGAAC AGCCTTTGTC CCTGGAATGG
    CCTGAGACCA GGCGGAAGCA AGCCATTTAC CTCTTTCTCC TGCCCATTGT GTTCCCGCTG
    TGGCTGACGG TTCCCGACGT CCGGAGGCTG GAGGCTAAGA AGTTTTTTGT TATCACCTTC
    CTGGGATCCA TCCTGTGGAT AGCCATGTTC TCATACCTCA TGGTTTGGTG GGCTCACCAG
    GTTGGCGAAA CAATAGGGAT TTCTGAAGAG ATCATGGGTT TGACGATCCT AGCAGCAGGC
    ACATCAATTC CTGACCTCAT CACCAGTGTG ATTGTCGCTC GGAAAGGCCT GGGTGACATG
    GCTGTGTCAA GCTCCGTGGG CAGTAACATA TTTGATATCA CCGTGGGCCT GCCCCTTCCT
    TGGATGCTTT TCTCTCTCAT CAATGGATTG CAGCCTGTTG CTGTCAGTAG CAATGGCTTG
    TTCTGTGCAA TTGTTTTGCT TTTTCTCATG CTCCTGTTTG TGATCTCGTC AATTGCATTA
    TGCAAGTGGA GAATGAACAA GATCCTGGGC TTCACCATGT TCCTCCTGTA CTTTGTATTC
    CTGATAATCA GTGTGATGCT GGAAGATCGA ATCATATCCT GCCCTGTATC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45446
    Clone ID Related Accession (Same CDS sequence) XM_010808867.2
    Accession Version XM_010808867.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGAAGGT GGTGAAGTGA AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGGGAAGTG GAAGGTGATG AAGACGAAGG TGAAATCCAG
    GCAGGAGAAG GTGGGGAAGT GGAAGGTGAT GAAGACGAAG GTGAAATCCA GGCAGGAGAA
    GGTGGGGAAG TGGAAGGTGA TGAAGACGAA GGTGAAATCC AGGCAGGAGA AGCTGGGGAA
    GTGGAAGGTG ATGAAGACGA AGGTGAAATC CAGGCAGGAG AAGCTGGGGA AGTGGAAGGT
    GATGAAGACG AAGGTGAAAT CCAGGCAGGA GAAGGTGGTG AAGTGAAAGG TGATGAAGGT
    GAAATCCAGG CAGGAGAAGC TGGGGAAGTG GAAGGAGAGG ATGGTGAAGT GGAAGGTGGT
    GAAGATGAAG GTGAAATCCA GGCAGGAGAA GGTGGTGAAG GTGAAACTGG AGAGCAGGAA
    TTGAATGCTG AAATTCAAGG TGAAGCCAAA GATGACGAGG AAGGTGTGGA TGGTGAAGGG
    GGAGGTGATG GAGGTGACAG TGAAGATGAA GAAGAGGAAG ATGAAGAAGA GGAAGACGAA
    GAAGAGGAAG AGGAGGAAGA GGAGGAGGAA GAGGAGGAAA ATGAACAGCC TTTGTCCCTG
    GAATGGCCTG AGACCAGGCG GAAGCAAGCC ATTTACCTCT TTCTCCTGCC CATTGTGTTC
    CCGCTGTGGC TGACGGTTCC CGACGTCCGG AGGCTGGAGG CTAAGAAGTT TTTTGTTATC
    ACCTTCCTGG GATCCATCCT GTGGATAGCC ATGTTCTCAT ACCTCATGGT TTGGTGGGCT
    CACCAGGTTG GCGAAACAAT AGGGATTTCT GAAGAGATCA TGGGTTTGAC GATCCTAGCA
    GCAGGCACAT CAATTCCTGA CCTCATCACC AGTGTGATTG TCGCTCGGAA AGGCCTGGGT
    GACATGGCTG TGTCAAGCTC CGTGGGCAGT AACATATTTG ATATCACCGT GGGCCTGCCC
    CTTCCTTGGA TGCTTTTCTC TCTCATCAAT GGATTGCAGC CTGTTGCTGT CAGTAGCAAT
    GGCTTGTTCT GTGCAATTGT TTTGCTTTTT CTCATGCTCC TGTTTGTGAT CTCGTCAATT
    GCATTATGCA AGTGGAGAAT GAACAAGATC CTGGGCTTCA CCATGTTCCT CCTGTACTTT
    GTATTCCTGA TAATCAGTGT GATGCTGGAA GATCGAATCA TATCCTGCCC TGTATCTGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807169.1
    CDS280..4002
    Translation

    Target ORF information:

    RefSeq Version XM_010808867.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808867.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGAAGGT GGTGAAGTGA AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGGGAAGTG GAAGGTGATG AAGACGAAGG TGAAATCCAG
    GCAGGAGAAG GTGGGGAAGT GGAAGGTGAT GAAGACGAAG GTGAAATCCA GGCAGGAGAA
    GGTGGGGAAG TGGAAGGTGA TGAAGACGAA GGTGAAATCC AGGCAGGAGA AGCTGGGGAA
    GTGGAAGGTG ATGAAGACGA AGGTGAAATC CAGGCAGGAG AAGCTGGGGA AGTGGAAGGT
    GATGAAGACG AAGGTGAAAT CCAGGCAGGA GAAGGTGGTG AAGTGAAAGG TGATGAAGGT
    GAAATCCAGG CAGGAGAAGC TGGGGAAGTG GAAGGAGAGG ATGGTGAAGT GGAAGGTGGT
    GAAGATGAAG GTGAAATCCA GGCAGGAGAA GGTGGTGAAG GTGAAACTGG AGAGCAGGAA
    TTGAATGCTG AAATTCAAGG TGAAGCCAAA GATGACGAGG AAGGTGTGGA TGGTGAAGGG
    GGAGGTGATG GAGGTGACAG TGAAGATGAA GAAGAGGAAG ATGAAGAAGA GGAAGACGAA
    GAAGAGGAAG AGGAGGAAGA GGAGGAGGAA GAGGAGGAAA ATGAACAGCC TTTGTCCCTG
    GAATGGCCTG AGACCAGGCG GAAGCAAGCC ATTTACCTCT TTCTCCTGCC CATTGTGTTC
    CCGCTGTGGC TGACGGTTCC CGACGTCCGG AGGCTGGAGG CTAAGAAGTT TTTTGTTATC
    ACCTTCCTGG GATCCATCCT GTGGATAGCC ATGTTCTCAT ACCTCATGGT TTGGTGGGCT
    CACCAGGTTG GCGAAACAAT AGGGATTTCT GAAGAGATCA TGGGTTTGAC GATCCTAGCA
    GCAGGCACAT CAATTCCTGA CCTCATCACC AGTGTGATTG TCGCTCGGAA AGGCCTGGGT
    GACATGGCTG TGTCAAGCTC CGTGGGCAGT AACATATTTG ATATCACCGT GGGCCTGCCC
    CTTCCTTGGA TGCTTTTCTC TCTCATCAAT GGATTGCAGC CTGTTGCTGT CAGTAGCAAT
    GGCTTGTTCT GTGCAATTGT TTTGCTTTTT CTCATGCTCC TGTTTGTGAT CTCGTCAATT
    GCATTATGCA AGTGGAGAAT GAACAAGATC CTGGGCTTCA CCATGTTCCT CCTGTACTTT
    GTATTCCTGA TAATCAGTGT GATGCTGGAA GATCGAATCA TATCCTGCCC TGTATCTGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45448
    Clone ID Related Accession (Same CDS sequence) XM_010808872.2
    Accession Version XM_010808872.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808872.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGAAGGT GGTGAAGTGA AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGGGAAGTG GAAGGTGATG AAGACGAAGG TGAAATCCAG
    GCAGGAGAAG GTGGGGAAGT GGAAGGTGAT GAAGACGAAG GTGAAATCCA GGCAGGAGAA
    GGTGGGGAAG TGGAAGGTGA TGAAGACGAA GGTGAAATCC AGGCAGGAGA AGCTGGGGAA
    GTGGAAGGTG ATGAAGACGA AGGTGAAATC CAGGCAGGAG AAGCTGGGGA AGTGGAAGGA
    GAGGATGGTG AAGTGGAAGG TGGTGAAGAT GAAGGTGAAA TCCAGGCAGG AGAAGGTGGT
    GAAGGTGAAA CTGGAGAGCA GGAATTGAAT GCTGAAATTC AAGGTGAAGC CAAAGATGAC
    GAGGAAGGTG TGGATGGTGA AGGGGGAGGT GATGGAGGTG ACAGTGAAGA TGAAGAAGAG
    GAAGATGAAG AAGAGGAAGA CGAAGAAGAG GAAGAGGAGG AAGAGGAGGA GGAAGAGGAG
    GAAAATGAAC AGCCTTTGTC CCTGGAATGG CCTGAGACCA GGCGGAAGCA AGCCATTTAC
    CTCTTTCTCC TGCCCATTGT GTTCCCGCTG TGGCTGACGG TTCCCGACGT CCGGAGGCTG
    GAGGCTAAGA AGTTTTTTGT TATCACCTTC CTGGGATCCA TCCTGTGGAT AGCCATGTTC
    TCATACCTCA TGGTTTGGTG GGCTCACCAG GTTGGCGAAA CAATAGGGAT TTCTGAAGAG
    ATCATGGGTT TGACGATCCT AGCAGCAGGC ACATCAATTC CTGACCTCAT CACCAGTGTG
    ATTGTCGCTC GGAAAGGCCT GGGTGACATG GCTGTGTCAA GCTCCGTGGG CAGTAACATA
    TTTGATATCA CCGTGGGCCT GCCCCTTCCT TGGATGCTTT TCTCTCTCAT CAATGGATTG
    CAGCCTGTTG CTGTCAGTAG CAATGGCTTG TTCTGTGCAA TTGTTTTGCT TTTTCTCATG
    CTCCTGTTTG TGATCTCGTC AATTGCATTA TGCAAGTGGA GAATGAACAA GATCCTGGGC
    TTCACCATGT TCCTCCTGTA CTTTGTATTC CTGATAATCA GTGTGATGCT GGAAGATCGA
    ATCATATCCT GCCCTGTATC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807174.1
    CDS280..3906
    Translation

    Target ORF information:

    RefSeq Version XM_010808872.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808872.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGAAGGT GGTGAAGTGA AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGGGAAGTG GAAGGTGATG AAGACGAAGG TGAAATCCAG
    GCAGGAGAAG GTGGGGAAGT GGAAGGTGAT GAAGACGAAG GTGAAATCCA GGCAGGAGAA
    GGTGGGGAAG TGGAAGGTGA TGAAGACGAA GGTGAAATCC AGGCAGGAGA AGCTGGGGAA
    GTGGAAGGTG ATGAAGACGA AGGTGAAATC CAGGCAGGAG AAGCTGGGGA AGTGGAAGGA
    GAGGATGGTG AAGTGGAAGG TGGTGAAGAT GAAGGTGAAA TCCAGGCAGG AGAAGGTGGT
    GAAGGTGAAA CTGGAGAGCA GGAATTGAAT GCTGAAATTC AAGGTGAAGC CAAAGATGAC
    GAGGAAGGTG TGGATGGTGA AGGGGGAGGT GATGGAGGTG ACAGTGAAGA TGAAGAAGAG
    GAAGATGAAG AAGAGGAAGA CGAAGAAGAG GAAGAGGAGG AAGAGGAGGA GGAAGAGGAG
    GAAAATGAAC AGCCTTTGTC CCTGGAATGG CCTGAGACCA GGCGGAAGCA AGCCATTTAC
    CTCTTTCTCC TGCCCATTGT GTTCCCGCTG TGGCTGACGG TTCCCGACGT CCGGAGGCTG
    GAGGCTAAGA AGTTTTTTGT TATCACCTTC CTGGGATCCA TCCTGTGGAT AGCCATGTTC
    TCATACCTCA TGGTTTGGTG GGCTCACCAG GTTGGCGAAA CAATAGGGAT TTCTGAAGAG
    ATCATGGGTT TGACGATCCT AGCAGCAGGC ACATCAATTC CTGACCTCAT CACCAGTGTG
    ATTGTCGCTC GGAAAGGCCT GGGTGACATG GCTGTGTCAA GCTCCGTGGG CAGTAACATA
    TTTGATATCA CCGTGGGCCT GCCCCTTCCT TGGATGCTTT TCTCTCTCAT CAATGGATTG
    CAGCCTGTTG CTGTCAGTAG CAATGGCTTG TTCTGTGCAA TTGTTTTGCT TTTTCTCATG
    CTCCTGTTTG TGATCTCGTC AATTGCATTA TGCAAGTGGA GAATGAACAA GATCCTGGGC
    TTCACCATGT TCCTCCTGTA CTTTGTATTC CTGATAATCA GTGTGATGCT GGAAGATCGA
    ATCATATCCT GCCCTGTATC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45449
    Clone ID Related Accession (Same CDS sequence) XM_010808874.2
    Accession Version XM_010808874.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGAAGGT GGTGAAGTGA AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGGGAAGTG GAAGGTGATG AAGACGAAGG TGAAATCCAG
    GCAGGAGAAG CTGGGGAAGT GGAAGGAGAG GATGGTGAAG TGGAAGGTGG TGAAGATGAA
    GGTGAAATCC AGGCAGGAGA AGGTGGTGAA GGTGAAACTG GAGAGCAGGA ATTGAATGCT
    GAAATTCAAG GTGAAGCCAA AGATGACGAG GAAGGTGTGG ATGGTGAAGG GGGAGGTGAT
    GGAGGTGACA GTGAAGATGA AGAAGAGGAA GATGAAGAAG AGGAAGACGA AGAAGAGGAA
    GAGGAGGAAG AGGAGGAGGA AGAGGAGGAA AATGAACAGC CTTTGTCCCT GGAATGGCCT
    GAGACCAGGC GGAAGCAAGC CATTTACCTC TTTCTCCTGC CCATTGTGTT CCCGCTGTGG
    CTGACGGTTC CCGACGTCCG GAGGCTGGAG GCTAAGAAGT TTTTTGTTAT CACCTTCCTG
    GGATCCATCC TGTGGATAGC CATGTTCTCA TACCTCATGG TTTGGTGGGC TCACCAGGTT
    GGCGAAACAA TAGGGATTTC TGAAGAGATC ATGGGTTTGA CGATCCTAGC AGCAGGCACA
    TCAATTCCTG ACCTCATCAC CAGTGTGATT GTCGCTCGGA AAGGCCTGGG TGACATGGCT
    GTGTCAAGCT CCGTGGGCAG TAACATATTT GATATCACCG TGGGCCTGCC CCTTCCTTGG
    ATGCTTTTCT CTCTCATCAA TGGATTGCAG CCTGTTGCTG TCAGTAGCAA TGGCTTGTTC
    TGTGCAATTG TTTTGCTTTT TCTCATGCTC CTGTTTGTGA TCTCGTCAAT TGCATTATGC
    AAGTGGAGAA TGAACAAGAT CCTGGGCTTC ACCATGTTCC TCCTGTACTT TGTATTCCTG
    ATAATCAGTG TGATGCTGGA AGATCGAATC ATATCCTGCC CTGTATCTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807176.1
    CDS280..3753
    Translation

    Target ORF information:

    RefSeq Version XM_010808874.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808874.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGAAGGT GGTGAAGTGA AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGGGAAGTG GAAGGTGATG AAGACGAAGG TGAAATCCAG
    GCAGGAGAAG CTGGGGAAGT GGAAGGAGAG GATGGTGAAG TGGAAGGTGG TGAAGATGAA
    GGTGAAATCC AGGCAGGAGA AGGTGGTGAA GGTGAAACTG GAGAGCAGGA ATTGAATGCT
    GAAATTCAAG GTGAAGCCAA AGATGACGAG GAAGGTGTGG ATGGTGAAGG GGGAGGTGAT
    GGAGGTGACA GTGAAGATGA AGAAGAGGAA GATGAAGAAG AGGAAGACGA AGAAGAGGAA
    GAGGAGGAAG AGGAGGAGGA AGAGGAGGAA AATGAACAGC CTTTGTCCCT GGAATGGCCT
    GAGACCAGGC GGAAGCAAGC CATTTACCTC TTTCTCCTGC CCATTGTGTT CCCGCTGTGG
    CTGACGGTTC CCGACGTCCG GAGGCTGGAG GCTAAGAAGT TTTTTGTTAT CACCTTCCTG
    GGATCCATCC TGTGGATAGC CATGTTCTCA TACCTCATGG TTTGGTGGGC TCACCAGGTT
    GGCGAAACAA TAGGGATTTC TGAAGAGATC ATGGGTTTGA CGATCCTAGC AGCAGGCACA
    TCAATTCCTG ACCTCATCAC CAGTGTGATT GTCGCTCGGA AAGGCCTGGG TGACATGGCT
    GTGTCAAGCT CCGTGGGCAG TAACATATTT GATATCACCG TGGGCCTGCC CCTTCCTTGG
    ATGCTTTTCT CTCTCATCAA TGGATTGCAG CCTGTTGCTG TCAGTAGCAA TGGCTTGTTC
    TGTGCAATTG TTTTGCTTTT TCTCATGCTC CTGTTTGTGA TCTCGTCAAT TGCATTATGC
    AAGTGGAGAA TGAACAAGAT CCTGGGCTTC ACCATGTTCC TCCTGTACTT TGTATTCCTG
    ATAATCAGTG TGATGCTGGA AGATCGAATC ATATCCTGCC CTGTATCTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45450
    Clone ID Related Accession (Same CDS sequence) XM_010808876.2
    Accession Version XM_010808876.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGAAGGT GGTGAAGTGA AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGC TGGGGAAGTG GAAGGAGAGG ATGGTGAAGT GGAAGGTGGT
    GAAGATGAAG GTGAAATCCA GGCAGGAGAA GGTGGTGAAG GTGAAACTGG AGAGCAGGAA
    TTGAATGCTG AAATTCAAGG TGAAGCCAAA GATGACGAGG AAGGTGTGGA TGGTGAAGGG
    GGAGGTGATG GAGGTGACAG TGAAGATGAA GAAGAGGAAG ATGAAGAAGA GGAAGACGAA
    GAAGAGGAAG AGGAGGAAGA GGAGGAGGAA GAGGAGGAAA ATGAACAGCC TTTGTCCCTG
    GAATGGCCTG AGACCAGGCG GAAGCAAGCC ATTTACCTCT TTCTCCTGCC CATTGTGTTC
    CCGCTGTGGC TGACGGTTCC CGACGTCCGG AGGCTGGAGG CTAAGAAGTT TTTTGTTATC
    ACCTTCCTGG GATCCATCCT GTGGATAGCC ATGTTCTCAT ACCTCATGGT TTGGTGGGCT
    CACCAGGTTG GCGAAACAAT AGGGATTTCT GAAGAGATCA TGGGTTTGAC GATCCTAGCA
    GCAGGCACAT CAATTCCTGA CCTCATCACC AGTGTGATTG TCGCTCGGAA AGGCCTGGGT
    GACATGGCTG TGTCAAGCTC CGTGGGCAGT AACATATTTG ATATCACCGT GGGCCTGCCC
    CTTCCTTGGA TGCTTTTCTC TCTCATCAAT GGATTGCAGC CTGTTGCTGT CAGTAGCAAT
    GGCTTGTTCT GTGCAATTGT TTTGCTTTTT CTCATGCTCC TGTTTGTGAT CTCGTCAATT
    GCATTATGCA AGTGGAGAAT GAACAAGATC CTGGGCTTCA CCATGTTCCT CCTGTACTTT
    GTATTCCTGA TAATCAGTGT GATGCTGGAA GATCGAATCA TATCCTGCCC TGTATCTGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807178.1
    CDS280..3702
    Translation

    Target ORF information:

    RefSeq Version XM_010808876.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808876.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGAAGGT GGTGAAGTGA AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGC TGGGGAAGTG GAAGGAGAGG ATGGTGAAGT GGAAGGTGGT
    GAAGATGAAG GTGAAATCCA GGCAGGAGAA GGTGGTGAAG GTGAAACTGG AGAGCAGGAA
    TTGAATGCTG AAATTCAAGG TGAAGCCAAA GATGACGAGG AAGGTGTGGA TGGTGAAGGG
    GGAGGTGATG GAGGTGACAG TGAAGATGAA GAAGAGGAAG ATGAAGAAGA GGAAGACGAA
    GAAGAGGAAG AGGAGGAAGA GGAGGAGGAA GAGGAGGAAA ATGAACAGCC TTTGTCCCTG
    GAATGGCCTG AGACCAGGCG GAAGCAAGCC ATTTACCTCT TTCTCCTGCC CATTGTGTTC
    CCGCTGTGGC TGACGGTTCC CGACGTCCGG AGGCTGGAGG CTAAGAAGTT TTTTGTTATC
    ACCTTCCTGG GATCCATCCT GTGGATAGCC ATGTTCTCAT ACCTCATGGT TTGGTGGGCT
    CACCAGGTTG GCGAAACAAT AGGGATTTCT GAAGAGATCA TGGGTTTGAC GATCCTAGCA
    GCAGGCACAT CAATTCCTGA CCTCATCACC AGTGTGATTG TCGCTCGGAA AGGCCTGGGT
    GACATGGCTG TGTCAAGCTC CGTGGGCAGT AACATATTTG ATATCACCGT GGGCCTGCCC
    CTTCCTTGGA TGCTTTTCTC TCTCATCAAT GGATTGCAGC CTGTTGCTGT CAGTAGCAAT
    GGCTTGTTCT GTGCAATTGT TTTGCTTTTT CTCATGCTCC TGTTTGTGAT CTCGTCAATT
    GCATTATGCA AGTGGAGAAT GAACAAGATC CTGGGCTTCA CCATGTTCCT CCTGTACTTT
    GTATTCCTGA TAATCAGTGT GATGCTGGAA GATCGAATCA TATCCTGCCC TGTATCTGTC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45451
    Clone ID Related Accession (Same CDS sequence) XM_010808877.2
    Accession Version XM_010808877.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3375bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GCTGGGGAAG TGGAAGGAGA GGATGGTGAA
    GTGGAAGGTG GTGAAGATGA AGGTGAAATC CAGGCAGGAG AAGGTGGTGA AGGTGAAACT
    GGAGAGCAGG AATTGAATGC TGAAATTCAA GGTGAAGCCA AAGATGACGA GGAAGGTGTG
    GATGGTGAAG GGGGAGGTGA TGGAGGTGAC AGTGAAGATG AAGAAGAGGA AGATGAAGAA
    GAGGAAGACG AAGAAGAGGA AGAGGAGGAA GAGGAGGAGG AAGAGGAGGA AAATGAACAG
    CCTTTGTCCC TGGAATGGCC TGAGACCAGG CGGAAGCAAG CCATTTACCT CTTTCTCCTG
    CCCATTGTGT TCCCGCTGTG GCTGACGGTT CCCGACGTCC GGAGGCTGGA GGCTAAGAAG
    TTTTTTGTTA TCACCTTCCT GGGATCCATC CTGTGGATAG CCATGTTCTC ATACCTCATG
    GTTTGGTGGG CTCACCAGGT TGGCGAAACA ATAGGGATTT CTGAAGAGAT CATGGGTTTG
    ACGATCCTAG CAGCAGGCAC ATCAATTCCT GACCTCATCA CCAGTGTGAT TGTCGCTCGG
    AAAGGCCTGG GTGACATGGC TGTGTCAAGC TCCGTGGGCA GTAACATATT TGATATCACC
    GTGGGCCTGC CCCTTCCTTG GATGCTTTTC TCTCTCATCA ATGGATTGCA GCCTGTTGCT
    GTCAGTAGCA ATGGCTTGTT CTGTGCAATT GTTTTGCTTT TTCTCATGCT CCTGTTTGTG
    ATCTCGTCAA TTGCATTATG CAAGTGGAGA ATGAACAAGA TCCTGGGCTT CACCATGTTC
    CTCCTGTACT TTGTATTCCT GATAATCAGT GTGATGCTGG AAGATCGAAT CATATCCTGC
    CCTGTATCTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807179.1
    CDS280..3654
    Translation

    Target ORF information:

    RefSeq Version XM_010808877.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808877.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGGAAAC TGATCAGGAT GGGGGCACAG GAGAGGCGGT CACTCTGGCC AAAGCGACTT 
    CATTGGAGTC GCCCCCTCTT CCTATTGGGA ATGTTGATCA TAGGTTCCAC CTACCAGTAC
    CTGACGAGCC CTCAGGGCCT CCCCACACTG TGGGCAGCAG TCTCTTCCCA ACACCCTGTA
    AAAGTAGCCA GCCGGGACCT TTCCAACAAG GAGATGATGA TGGTGAGCAG TGAGACTTCA
    AAATCTAGCT CTGAAATGGA GGTTGAGGCA TGGGCACCGG AAGCCACAGC AGGCAGAGAT
    GGAACACCAC CTGGCATAGC CAGGAAGAAC ACCCCCAGTA CACCTAGAGG AACAGCCAGC
    ATCACTCCAG CAATACCCAA TAACTACAGC CCAACACCAA CAGGCACAGG AAAAGTTAAG
    GAAGACACCT CAGCCACACC CAGTGGAGTA CTGAATCACT ACACTCAAAG CAGACCAATG
    GTAAATAGTT ATACCCGCTT GACAGCCAGG GGAGAAGTAA AGAGCTCCCG CCCAACTCAA
    TCCAGGGGAA AGGAGGAGAA ATACTCCCCA TCCCCACTTG GTAGAATGGT AAACAGTTAT
    GCCCCATCCA CACTCATGAC AATGCCAAGG AGCCATGGCA TCACCCCCAG AACAACAGTG
    AAAGACAGAG AAATTATGGC GACCAAGAAA ATGTTGGCGA CCAACCCATC CAAGAGACTA
    GTGGAGAAAA CCACCCCAAC TCCTCTCAAG GGAATAACTG ATAACACCCC AACCTTCCTG
    TTAAGTGACT TAGAAACAGA CACCTTGACT TCTCCAAGAA ATGTGGTAGA AAAGAAGACC
    CTAACTACCC CCAGAAGAGT GGACAGTAAT AGCTCAACCA ACCACCAGGG GTTGGTGGGG
    AAAAACAATC TGACAACACC CCAAGGAATG GTCCTGGAGC ACACTGCGGC CGTCTCTGAG
    GGGCAAGTGA CAATAAGCAC CATGACACGC AGCAGCCCAA CAGAAACCAA AGCTTCTACT
    GATGCTTGGA AAGTCAGGAA TCCCTTGCCC AGAACCAGTG CACCGATCAT CAGAATATCC
    TCAGCCACCT TCAGGGGGCT TTTGAAAAAC CCTTCCAAAG CACCCAGCAC CCCAGCAGCT
    CCCAGGGTTA GGGCAAATCC GACCATTCAG GTCCGCCACT GCCTGGTGGT GGAGCCAGCC
    CCAGTTGCAC CCACTGCCCC CTCCCCTAGT TGGACAACAG CTGTGATCCC AGGGATTCCT
    AGTCCCTCAG GACAGCCAGA CCTGTACCCC AAGGCAGAGT ACCCCCGAGA CCTGTTCAGT
    GTGGAGGAGC GGAGGCAGGG CTGGGTGGTC CTGCACATCT TTGGCATGAT GTATGTGTTT
    GTAGCCTTGG CCATCGTTTG TGACGAGTAC TTTGTCCCTG CCCTGGGCGT CATCACAGAC
    AAGCTGCAGA TCTCTGAGGA TGTGGCAGGC GCTACATTCA TGGCTGCTGG AGGCTCTGCC
    CCCGAGCTCT TCACCTCCCT CATCGGCGTC TTCATCTCCC ATAGCAACGT GGGCATTGGC
    ACCATAGTGG GCTCTGCGGT GTTCAACATC CTCTTTGTCA TTGGCACTTG TGCCCTCTTC
    TCCCGGGAGA TCCTCAACCT CACCTGGTGG CCCTTGTTCC GAGACATCAC CTTCTACATC
    TTTGACCTAA TGATGCTCAT CCTCTTCTTC CTGGATAGCC TCATTGCATG GTGGGAGAGC
    GTGCTCCTGC TGCTGGCTTA TGCCTTCTAT GTGTTCACCA TGAAGTGGAA CCAACAGCTC
    GAGCTCTGGG TGAAAGAGCA GCTCAACAAG AGGCCAGTGG CCAAGGTCAT GGCCCTAGGG
    GACCTCAGCA AGCCGGGCGA TGGGACAGTT GTGGTGGATG AGCAGCAGGA TAACAAGAAG
    CTGAAGGCCC CCCCGTGTGG CGCCTCCCCT GGCTTGGACG GGGGCCCACT GAGGCCTGTG
    CGGCTCTTTC CTGCTCAGCT TTCATCCATG CTGACCCGAG GGAGCAGCTC GGCCTCTCTG
    CACAACAGCA CCATCCGCAG CACCATCTAC CAGCTCATGC TCCACAGCCT GGACCCTCTG
    GGGGAAGCCC GGCCCTCCAA GGACAAGGAG GAGGAGACCT TGATTCCGGA GGCAAAAGCC
    ACGCCTCAGG CCAAAGCAGA GAGCAAACCA GAGGAGGAGC CGGCCAAGCT CCCTGAGGTC
    ACGGTCACAC CAGCCCCTGC TCCAGACGTC AAGGGAGATC AGGAGGAGGA TCCTGGCAGT
    CAGGGAGTCG GGGCCGAAGC TGAGAACACA GGTGAAAGGA CAGGTGGAGA GGCGGAAGCT
    CCTGCTGAAG GTGAAAACGG AGAGCGAAGT GGAGGCGACG CTGCATTAGG AGGTGAAAGT
    GAAGGAAAAG CAGAGAATGA AAGTGAAGGT GACATCCCGG CAGAAAGGAG AGGAGATGAT
    GAAGACGAAG GTGAAATCCA GGCAGGAGAA GCTGGGGAAG TGGAAGGAGA GGATGGTGAA
    GTGGAAGGTG GTGAAGATGA AGGTGAAATC CAGGCAGGAG AAGGTGGTGA AGGTGAAACT
    GGAGAGCAGG AATTGAATGC TGAAATTCAA GGTGAAGCCA AAGATGACGA GGAAGGTGTG
    GATGGTGAAG GGGGAGGTGA TGGAGGTGAC AGTGAAGATG AAGAAGAGGA AGATGAAGAA
    GAGGAAGACG AAGAAGAGGA AGAGGAGGAA GAGGAGGAGG AAGAGGAGGA AAATGAACAG
    CCTTTGTCCC TGGAATGGCC TGAGACCAGG CGGAAGCAAG CCATTTACCT CTTTCTCCTG
    CCCATTGTGT TCCCGCTGTG GCTGACGGTT CCCGACGTCC GGAGGCTGGA GGCTAAGAAG
    TTTTTTGTTA TCACCTTCCT GGGATCCATC CTGTGGATAG CCATGTTCTC ATACCTCATG
    GTTTGGTGGG CTCACCAGGT TGGCGAAACA ATAGGGATTT CTGAAGAGAT CATGGGTTTG
    ACGATCCTAG CAGCAGGCAC ATCAATTCCT GACCTCATCA CCAGTGTGAT TGTCGCTCGG
    AAAGGCCTGG GTGACATGGC TGTGTCAAGC TCCGTGGGCA GTAACATATT TGATATCACC
    GTGGGCCTGC CCCTTCCTTG GATGCTTTTC TCTCTCATCA ATGGATTGCA GCCTGTTGCT
    GTCAGTAGCA ATGGCTTGTT CTGTGCAATT GTTTTGCTTT TTCTCATGCT CCTGTTTGTG
    ATCTCGTCAA TTGCATTATG CAAGTGGAGA ATGAACAAGA TCCTGGGCTT CACCATGTTC
    CTCCTGTACT TTGTATTCCT GATAATCAGT GTGATGCTGG AAGATCGAAT CATATCCTGC
    CCTGTATCTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45452
    Clone ID Related Accession (Same CDS sequence) XM_010808878.2
    Accession Version XM_010808878.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sodium/potassium/calcium exchanger 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104268.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808878.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGTGGGAG AGCGTGCTCC TGCTGCTGGC TTATGCCTTC TATGTGTTCA CCATGAAGTG 
    GAACCAACAG CTCGAGCTCT GGGTGAAAGA GCAGCTCAAC AAGAGGCCAG TGGCCAAGGT
    CATGGCCCTA GGGGACCTCA GCAAGCCCGG CCCTCCAAGG ACAAGGAGGA GGAGACCTTG
    ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC AAAGCAGAGA GCAAACCAGA GGAGGAGCCG
    GCCAAGCTCC CTGAGGTCAC GGTCACACCA GCCCCTGCTC CAGACGTCAA GGGAGATCAG
    GAGGAGGATC CTGGCAGTCA GGGAGTCGGG GCCGAAGCTG AGAACACAGG TGAAAGGACA
    GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT GAAAACGGAG AGCGAAGTGG AGGCGACGCT
    GCATTAGGAG GTGAAAGTGA AGGAAAAGCA GAGAATGAAA GTGAAGGTGA CATCCCGGCA
    GAAAGGAGAG GAGATGATGA AGACGAAGGT GAAATCCAGG CAGAAGGTGG TGAAGTGAAA
    GGTGATGAAG ACGAAGGTGA AATCCAGGCA GGAGAAGGTG GGGAAGTGGA AGGTGATGAA
    GACGAAGGTG AAATCCAGGC AGGAGAAGGT GGGGAAGTGG AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGGGAAGTG GAAGGTGATG AAGACGAAGG TGAAATCCAG
    GCAGGAGAAG CTGGGGAAGT GGAAGGTGAT GAAGACGAAG GTGAAATCCA GGCAGGAGAA
    GCTGGGGAAG TGGAAGGTGA TGAAGACGAA GGTGAAATCC AGGCAGGAGA AGGTGGTGAA
    GTGAAAGGTG ATGAAGGTGA AATCCAGGCA GGAGAAGCTG GGGAAGTGGA AGGAGAGGAT
    GGTGAAGTGG AAGGTGGTGA AGATGAAGGT GAAATCCAGG CAGGAGAAGG TGGTGAAGGT
    GAAACTGGAG AGCAGGAATT GAATGCTGAA ATTCAAGGTG AAGCCAAAGA TGACGAGGAA
    GGTGTGGATG GTGAAGGGGG AGGTGATGGA GGTGACAGTG AAGATGAAGA AGAGGAAGAT
    GAAGAAGAGG AAGACGAAGA AGAGGAAGAG GAGGAAGAGG AGGAGGAAGA GGAGGAAAAT
    GAACAGCCTT TGTCCCTGGA ATGGCCTGAG ACCAGGCGGA AGCAAGCCAT TTACCTCTTT
    CTCCTGCCCA TTGTGTTCCC GCTGTGGCTG ACGGTTCCCG ACGTCCGGAG GCTGGAGGCT
    AAGAAGTTTT TTGTTATCAC CTTCCTGGGA TCCATCCTGT GGATAGCCAT GTTCTCATAC
    CTCATGGTTT GGTGGGCTCA CCAGGTTGGC GAAACAATAG GGATTTCTGA AGAGATCATG
    GGTTTGACGA TCCTAGCAGC AGGCACATCA ATTCCTGACC TCATCACCAG TGTGATTGTC
    GCTCGGAAAG GCCTGGGTGA CATGGCTGTG TCAAGCTCCG TGGGCAGTAA CATATTTGAT
    ATCACCGTGG GCCTGCCCCT TCCTTGGATG CTTTTCTCTC TCATCAATGG ATTGCAGCCT
    GTTGCTGTCA GTAGCAATGG CTTGTTCTGT GCAATTGTTT TGCTTTTTCT CATGCTCCTG
    TTTGTGATCT CGTCAATTGC ATTATGCAAG TGGAGAATGA ACAAGATCCT GGGCTTCACC
    ATGTTCCTCC TGTACTTTGT ATTCCTGATA ATCAGTGTGA TGCTGGAAGA TCGAATCATA
    TCCTGCCCTG TATCTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807180.1
    CDS105..1865
    Translation

    Target ORF information:

    RefSeq Version XM_010808878.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 24 (sodium/potassium/calcium exchanger), member 1 (SLC24A1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010808878.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGTGGGAG AGCGTGCTCC TGCTGCTGGC TTATGCCTTC TATGTGTTCA CCATGAAGTG 
    GAACCAACAG CTCGAGCTCT GGGTGAAAGA GCAGCTCAAC AAGAGGCCAG TGGCCAAGGT
    CATGGCCCTA GGGGACCTCA GCAAGCCCGG CCCTCCAAGG ACAAGGAGGA GGAGACCTTG
    ATTCCGGAGG CAAAAGCCAC GCCTCAGGCC AAAGCAGAGA GCAAACCAGA GGAGGAGCCG
    GCCAAGCTCC CTGAGGTCAC GGTCACACCA GCCCCTGCTC CAGACGTCAA GGGAGATCAG
    GAGGAGGATC CTGGCAGTCA GGGAGTCGGG GCCGAAGCTG AGAACACAGG TGAAAGGACA
    GGTGGAGAGG CGGAAGCTCC TGCTGAAGGT GAAAACGGAG AGCGAAGTGG AGGCGACGCT
    GCATTAGGAG GTGAAAGTGA AGGAAAAGCA GAGAATGAAA GTGAAGGTGA CATCCCGGCA
    GAAAGGAGAG GAGATGATGA AGACGAAGGT GAAATCCAGG CAGAAGGTGG TGAAGTGAAA
    GGTGATGAAG ACGAAGGTGA AATCCAGGCA GGAGAAGGTG GGGAAGTGGA AGGTGATGAA
    GACGAAGGTG AAATCCAGGC AGGAGAAGGT GGGGAAGTGG AAGGTGATGA AGACGAAGGT
    GAAATCCAGG CAGGAGAAGG TGGGGAAGTG GAAGGTGATG AAGACGAAGG TGAAATCCAG
    GCAGGAGAAG CTGGGGAAGT GGAAGGTGAT GAAGACGAAG GTGAAATCCA GGCAGGAGAA
    GCTGGGGAAG TGGAAGGTGA TGAAGACGAA GGTGAAATCC AGGCAGGAGA AGGTGGTGAA
    GTGAAAGGTG ATGAAGGTGA AATCCAGGCA GGAGAAGCTG GGGAAGTGGA AGGAGAGGAT
    GGTGAAGTGG AAGGTGGTGA AGATGAAGGT GAAATCCAGG CAGGAGAAGG TGGTGAAGGT
    GAAACTGGAG AGCAGGAATT GAATGCTGAA ATTCAAGGTG AAGCCAAAGA TGACGAGGAA
    GGTGTGGATG GTGAAGGGGG AGGTGATGGA GGTGACAGTG AAGATGAAGA AGAGGAAGAT
    GAAGAAGAGG AAGACGAAGA AGAGGAAGAG GAGGAAGAGG AGGAGGAAGA GGAGGAAAAT
    GAACAGCCTT TGTCCCTGGA ATGGCCTGAG ACCAGGCGGA AGCAAGCCAT TTACCTCTTT
    CTCCTGCCCA TTGTGTTCCC GCTGTGGCTG ACGGTTCCCG ACGTCCGGAG GCTGGAGGCT
    AAGAAGTTTT TTGTTATCAC CTTCCTGGGA TCCATCCTGT GGATAGCCAT GTTCTCATAC
    CTCATGGTTT GGTGGGCTCA CCAGGTTGGC GAAACAATAG GGATTTCTGA AGAGATCATG
    GGTTTGACGA TCCTAGCAGC AGGCACATCA ATTCCTGACC TCATCACCAG TGTGATTGTC
    GCTCGGAAAG GCCTGGGTGA CATGGCTGTG TCAAGCTCCG TGGGCAGTAA CATATTTGAT
    ATCACCGTGG GCCTGCCCCT TCCTTGGATG CTTTTCTCTC TCATCAATGG ATTGCAGCCT
    GTTGCTGTCA GTAGCAATGG CTTGTTCTGT GCAATTGTTT TGCTTTTTCT CATGCTCCTG
    TTTGTGATCT CGTCAATTGC ATTATGCAAG TGGAGAATGA ACAAGATCCT GGGCTTCACC
    ATGTTCCTCC TGTACTTTGT ATTCCTGATA ATCAGTGTGA TGCTGGAAGA TCGAATCATA
    TCCTGCCCTG TATCTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.