PLA2G1B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLA2G1B
Entrez Gene ID	282457
Full Name	phospholipase A2 group IB
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174646.3	NP_777071.2	phospholipase A2 preproprotein

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Pla2g1b	NP_113773.1
Xenopus tropicalis (tropical clawed frog)	pla2g1b	XP_004910660.1
	PLA2G1B	XP_001088684.1
Canis lupus familiaris (dog)	PLA2G1B	NP_001003320.1
Mus musculus (house mouse)	Pla2g1b	NP_035237.1
Gallus gallus (chicken)	PLA2G1B	XP_001233627.2
Homo sapiens (human)	PLA2G1B	NP_000919.1
Pan troglodytes (chimpanzee)	PLA2G1B	XP_001160305.1
Bos taurus (cattle)	PLA2G1B	NP_777071.2
Danio rerio (zebrafish)	pla2g1b	XP_700448.2

Gene Ontology
Bibliography

Related articles in PubMed

Differential participation of phospholipase A2 isoforms during iron-induced retinal toxicity. Implications for age-related macular degeneration.
Rodr?guez Diez G, Uranga RM, Mateos MV, Giusto NM, Salvador GA
Neurochemistry international61(5)749-58(2012 Oct)

Surfactant protein B inhibits secretory phospholipase A2 hydrolysis of surfactant phospholipids.
Hite RD, Grier BL, Waite BM, Veldhuizen RA, Possmayer F, Yao LJ, Seeds MC
American journal of physiology. Lung cellular and molecular physiology302(2)L257-65(2012 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Suggestive evidence for the involvement of the second calcium and surface loop in interfacial binding: monoclinic and trigonal crystal structures of a quadruple mutant of phospholipase A2.
Sekar K, Yogavel M, Kanaujia SP, Sharma A, Velmurugan D, Poi MJ, Dauter Z, Tsai MD
Acta crystallographica. Section D, Biological crystallography62(Pt 7)717-24(2006 Jul)

Third calcium ion found in an inhibitor-bound phospholipase A2.
Sekar K, Gayathri D, Velmurugan D, Jeyakanthan J, Yamane T, Poi MJ, Tsai MD
Acta crystallographica. Section D, Biological crystallography62(Pt 4)392-7(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phospholipase A2 isoforms are differentially regulated; they selectively participate in retinal signaling in an experimental model of age-related macular degeneration.
Title: Differential participation of phospholipase A2 isoforms during iron-induced retinal toxicity. Implications for age-related macular degeneration.

results suggest the hydrophobic surfactant protein, SP-B, protects alveolar surfactant phospholiopids from hydrolysis mediated by multiple sPLA(2) in both vesicles (alveolar subphase) and monomolecular films (air-liquid interface)
Title: Surfactant protein B inhibits secretory phospholipase A2 hydrolysis of surfactant phospholipids.

The quadruple mutant of phospholipase A2 has monoclinic or trigonal crystal structures and results suggested evidence for the involvement of the second calcium and surface loop in interfacial binding.
Title: Suggestive evidence for the involvement of the second calcium and surface loop in interfacial binding: monoclinic and trigonal crystal structures of a quadruple mutant of phospholipase A2.

The crystal structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2 complexed with an organic molecule, p-methoxybenzoic acid (anisic acid), is reported.
Title: Third calcium ion found in an inhibitor-bound phospholipase A2.

The final protein model consists of 123 amino-acid residues, two calcium ions, one chloride ion, 243 water molecules and six 2-methyl-2,4-pentanediol molecules.
Title: Atomic resolution (0.97 A) structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2.

The following PLA2G1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44252	NM_174646.3 Latest version!	Bos taurus phospholipase A2 group IB (PLA2G1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLA2G1B ( NM_174646.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777071.2 Bos taurus phospholipase A2 group IB (PLA2G1B), mRNA.

Price & Availability↑

CloneID	OBa44252
Clone ID Related Accession (Same CDS sequence)	NM_174646.3
Accession Version	NM_174646.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Bos taurus(cattle)
Product	phospholipase A2 preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN543510.1. On Mar 26, 2010 this sequence version replaced NM_174646.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DN543510.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145497, SAMN06299470 [ECO:0000348] ##Evidence-Data-END##

ATGAGACTCC TGGTGTTGGC TGCTCTGCTC ACAGTGGGCG CTGGCCAGGC CGGCCTCAAC 
TCGAGGGCAT TATGGCAGTT CAACGGCATG ATTAAGTGCA AGATCCCCAG CAGTGAACCA 
TTGCTGGATT TCAACAACTA TGGCTGCTAT TGTGGCCTGG GTGGATCAGG GACCCCTGTG 
GATGATCTGG ACAGGTGCTG CCAGACGCAC GACAACTGCT ACAAACAAGC CAAGAAACTG 
GACAGCTGTA AAGTCCTCGT GGACAATCCC TACACCAACA ACTACTCCTA CTCATGTTCT 
AACAATGAGA TCACCTGCAG CAGCGAAAAC AATGCCTGTG AGGCCTTCAT CTGCAACTGT 
GACCGCAATG CCGCCATCTG CTTCTCAAAG GTGCCATACA ACAAGGAGCA CAAGAACCTG 
GACAAGAAGA ACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777071.2
CDS	93..530
Translation	MRLLVLAALLTVGAGQAGLNSRALWQFNGMIKCKIPSSEPLLDFNNYGCYCGLGGSGTPVDDLDRCCQTHDNCYKQAKKLDSCKVLVDNPYTNNYSYSCSNNEITCSSENNACEAFICNCDRNAAICFSKVPYNKEHKNLDKKNC

Target ORF information:

RefSeq Version	NM_174646.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase A2 group IB (PLA2G1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174646.3

ORF Insert Sequence:

ATGAGACTCC TGGTGTTGGC TGCTCTGCTC ACAGTGGGCG CTGGCCAGGC CGGCCTCAAC 
TCGAGGGCAT TATGGCAGTT CAACGGCATG ATTAAGTGCA AGATCCCCAG CAGTGAACCA 
TTGCTGGATT TCAACAACTA TGGCTGCTAT TGTGGCCTGG GTGGATCAGG GACCCCTGTG 
GATGATCTGG ACAGGTGCTG CCAGACGCAC GACAACTGCT ACAAACAAGC CAAGAAACTG 
GACAGCTGTA AAGTCCTCGT GGACAATCCC TACACCAACA ACTACTCCTA CTCATGTTCT 
AACAATGAGA TCACCTGCAG CAGCGAAAAC AATGCCTGTG AGGCCTTCAT CTGCAACTGT 
GACCGCAATG CCGCCATCTG CTTCTCAAAG GTGCCATACA ACAAGGAGCA CAAGAACCTG 
GACAAGAAGA ACTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential participation of phospholipase A2 isoforms during iron-induced retinal toxicity. Implications for age-related macular degeneration.
Neurochemistry international61(5)749-58(2012 Oct)
Rodr?guez Diez G,Uranga RM,Mateos MV,Giusto NM,Salvador GA

Surfactant protein B inhibits secretory phospholipase A2 hydrolysis of surfactant phospholipids.
American journal of physiology. Lung cellular and molecular physiology302(2)L257-65(2012 Jan)
Hite RD,Grier BL,Waite BM,Veldhuizen RA,Possmayer F,Yao LJ,Seeds MC

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Suggestive evidence for the involvement of the second calcium and surface loop in interfacial binding: monoclinic and trigonal crystal structures of a quadruple mutant of phospholipase A2.
Acta crystallographica. Section D, Biological crystallography62(Pt 7)717-24(2006 Jul)
Sekar K,Yogavel M,Kanaujia SP,Sharma A,Velmurugan D,Poi MJ,Dauter Z,Tsai MD

Third calcium ion found in an inhibitor-bound phospholipase A2.
Acta crystallographica. Section D, Biological crystallography62(Pt 4)392-7(2006 Apr)
Sekar K,Gayathri D,Velmurugan D,Jeyakanthan J,Yamane T,Poi MJ,Tsai MD

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PLA2G1B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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