CACNA1I cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1I
Entrez Gene ID	282413
Full Name	calcium voltage-gated channel subunit alpha1 I
Synonyms	CACNA1L
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015463606.1	XP_015319092.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X4
XM_024992917.1	XP_024848685.1	voltage-dependent T-type calcium channel subunit alpha-1I
XM_010805738.2	XP_010804040.2	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
XM_015463607.1	XP_015319093.1	voltage-dependent T-type calcium channel subunit alpha-1I isoform X5
XM_010805740.2	XP_010804042.2	voltage-dependent T-type calcium channel subunit alpha-1I isoform X3
XM_010805739.2	XP_010804041.2	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CACNA1I	XP_425474.3
Pan troglodytes (chimpanzee)	CACNA1I	XP_515143.4
Canis lupus familiaris (dog)	CACNA1I	XP_538364.4
Homo sapiens (human)	CACNA1I	NP_001003406.1
Bos taurus (cattle)	CACNA1I	XP_002687981.2
Mus musculus (house mouse)	Cacna1i	NP_001037773.2
Xenopus tropicalis (tropical clawed frog)	cacna1i	XP_002933803.2
Rattus norvegicus (Norway rat)	Cacna1i	NP_064469.3
Danio rerio (zebrafish)	cacna1i	XP_694715.5

Gene Ontology

The following CACNA1I gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1I cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235060	XM_024992917.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa90014	XM_015463606.1 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90015	XM_015463607.1 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90823	XM_010805738.2 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90824	XM_010805739.2 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa90825	XM_010805740.2 Latest version!	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1I ( XM_024992917.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848685.1 Bos taurus calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.

Price & Availability↑

CloneID	OBa235060
Clone ID Related Accession (Same CDS sequence)	XM_024992917.1
Accession Version	XM_024992917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1I
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGTTTGCCC TGGAGATGCT GCTGAAGCTG GCCGCCTTCG GGCTCTTCGA CTACCTGCGC 
AACCCCTATA ACATCTTCGA CAGCATCATC GTCATCATCA GCATCTGGGA GATCGTGGGG 
CAGGCGGACG GCGGGCTGTC AGTGCTGCGG ACTTTCCGGC TACTGCGGGT GCTGAAGCTG 
GTGCGCTTTA TGCCCGCGCT GCGGCGCCAG CTCGTGGTGC TCATGAAGAC CATGGACAAC 
GTGGCCACCT TCTGCATGCT GCTCATGCTT TTCATCTTCA TCTTCAGCAT CCTCGGGATG 
CACATCTTTG GCTGCAAATT CAGCCTGCGC ACGGACACGG GAGACACGGT CCCCGACAGG 
AAGAACTTTG ACTCCCTGCT GTGGGCCATC GTCACGGTGT TCCAGATCCT CACCCAGGAG 
GACTGGAACG TCGTCCTCTA CAACGGCATG GCTTCCACAT CCCCCTGGGC CTCCCTCTAT 
TTCGTTGCCC TCATGACCTT TGGCAATTAT GTGCTCTTCA ACCTGCTGGT GGCCATCCTG 
GTGGAGGGCT TCCAGGCGGA GGGTGATGCC AATCGCTCCT ACTCGGACGA GGACCAGAGC 
TCATCCAACA TGGAGGAGTT TGACAAGCTC CAGGAGGGCC TGGACTGCAG CGGAGATCCC 
AAGCTCTGCC CAATCCCCAT GACCCCCAAT GGGCACCTGG ACCCCAATCT CCCACTGGGT 
GGACATCTGG GCCCCACTGG GGCTGCAGCA TCTGCCCCCC GCCTCTCACT GCAGCCAGAC 
CCCGTGCTAA TGGCCCTGGG CTCCCGCAAG AGCAGTGTCA TGTCACTGGG GAGGATGAGC 
TACGACCAGC GCTCCCTGTC CAGCTCTCGG AGCTCCTACT ATGGGCCGTG GGGCCGCAGC 
GGGGCCTGGG CCAGCCGTCG CTCCAGCTGG AACAGCCTCA AGCACAAGCA ACCGTCGGCG 
GAGCATGAGT CCCTGCTCTC CGCTGAGCGT GGCGGCGCCC GGGGCTGCGA GGGCGCCCGG 
GACGAGGGCC CGCTACGGAC CGCGCCCCTG CACGCCCCGC ACGCCCACCA TGCGCATCAT 
GGTCCCCACC CGGTGCATCG TCACCACCAC CACCGCCGAA CGCTATCCCT CGACACCAGA 
GATTCGGTGG ACCTGGCCGA GCTGGTGCCC GCCGTGGGCA CCCACTCCCG GGCGGCCTGG 
AGGGCGGCAG GCCTGGCCCC CGGGCACGAG GACTGCAATG GCAGGATGCC CAGCATCGCC 
AAGGACGTCT TCACCAAGAT GGATGACCGC CGGGATCGCG GGGAGGACGA GGAGGAGATC 
GACTATACTC TGTGCTTCCG AGTCCGCAAG ATGATTGACG TCTACAAGCC TGACTGGTGT 
GAAGTCCGAG AGGACTGGTC CGTCTACCTC TTCTCCCCCG AGAACAGGTT CCGGGTCCTG 
TGTCAGACCA TCATTGCCCA CAAGCTCTTC GACTATGTCG TCCTGGCCTT CATCTTCCTC 
AACTGCATCA CCATCGCCCT GGAGCGGCCC CAGATTGAGG CTGGCAGCAC CGAACGCATC 
TTCCTCACTG TGTCCAACTA CATCTTCACA GCTATCTTCG TGGGCGAGAT GACACTGAAG 
GTGGTCTCGC TGGGCCTGTA CTTCGGGGAG CAGGCGTACC TGCGCAGCAG CTGGAACGTA 
CTGGACGGCT TCCTCGTCTT CGTGTCAATC ATTGACATTG TGGTGTCCGT GGCCTCGGCT 
GGGGGAGCCA AGATCTTGGG CGTCCTCCGG GTCCTGCGGC TCCTGCGCAC CCTACGACCC 
CTGCGTGTCA TCAGCCGGGC ACCTGGCCTG AAGCTGGTGG TGGAGACACT CATCTCCTCC 
CTAAAGCCCA TCGGCAACAT CGTCCTCATC TGCTGCGCCT TCTTCATCAT CTTCGGCATC 
CTGGGGGTGC AGCTCTTCAA GGGCAAGTTC TACCACTGTC TGGGTGTAGA CACCCGCAAC 
ATCACCAACC GCTCTGACTG CATGGCTGCC AACTACCGCT GGGTCCATCA CAAGTACAAC 
TTCGACAATC TGGGCCAGGC CCTCATGTCC CTCTTCGTGC TGGCCTCCAA GGATGGCTGG 
GTGAACATTA TGTACAATGG ACTGGACGCC GTCGCGGTGG ACCAGCAGCC CGTGCCCAAC 
CACAACCCCT GGATGCTGCT CTACTTCATC TCCTTCCTGC TCATCGTCAG CTTCTTCGTG 
CTCAACATGT TCGTGGGTGT CGTGGTGGAG AACTTCCACA AGTGCCGGCA GCACCAGGAG 
GCCGAGGAGG CGCGGCGGCG CGAGGAGAAG CGGCTGCGGC GGCTGGAGAA GAAGCGCCGG 
AAGGCCCAGC GGCTGCCCTA CTACGCTACC TACTGTCCCA CGAGGCTGCT CATCCACTCC 
ATGTGCACCA GCCACTACCT GGACATCTTC ATCACCTTCA TCATCTGCCT CAACGTGGTC 
ACCATGTCCC TGGAGCACTA CAACCAGCCC ACGTCTCTGG AGACAGCCCT CAAGTACTGC 
AACTACATGT TCACCACTGT CTTTGTGCTG GAGGCTGTGC TGAAGCTGGT GGCATTTGGT 
CTGAGACGCT TCTTCAAGGA CCGGTGGAAC CAACTGGACC TGGCCATCGT GCTGCTGTCG 
GTCATGGGCA TCACGCTGGA GGAGATTGAG ATCAACGCGG CGCTGCCCAT CAACCCCACC 
ATCATCCGAA TCATGCGGGT CCTGCGCATC GCCCGGGTGC TGAAGCTCCT GAAGATGGCC 
ACGGGAATGC GGGCCCTGCT GGACACTGTG GTGCAAGCTC TGCCCCAGGT GGGCAACCTG 
GGCCTCCTCT TCATGCTGCT CTTCTTCATC TACGCCGCCC TGGGAGTGGA GCTCTTCGGG 
AAGCTGGTCT GCAACGACGA GAACCCTTGT GAGGGCATGA GCCGGCACGC CACCTTCGAG 
AACTTCGGCA TGGCCTTCCT CACGCTCTTC CAGGTCTCCA CTGGCGACAA CTGGAATGGG 
ATCATGAAGG ACACACTGCG GGACTGCACG CACGACGAGC GCAGCTGCCT GAGCAGCCTG 
CAGTTCGTGT CACCGCTGTA CTTCGTCAGC TTCGTGCTCA CGGCCCAGTT CGTGCTCATC 
AACGTGGTGG TGGCCGTGCT CATGAAGCAC CTGGACGACA GCAACAAGGA GGCCCAGGAG 
GACGCCGAGA TGGACGCCGA GCTGGAGCTG GAGCTGGCCC ACGGCCTGGG CCCCAGCCCG 
CGGCCACCGG CCAGTGCCCC GGGGGCGCCC GCACCCGCCC ACGGGCCAGG AGGGGCGGCC 
GGCGAGGGTG ATCCCGAGGG CCGCCTGCGC CGAAGCTGCT ACTCGCCAGC CCAGGAGAAC 
CTGTGGCTGG ACAGCGTCTC TTTAATCATC AAGGACTCCT TGGAGGGGGA GCTGACCATC 
ATCGACAACC TGTCCGGGTC CATCTTCCAC CACTACTCCT CGCCGGCCGG CTGCGAGAAG 
TGTCACCACG ATAAGCAGGA GGTGCAGCTG GCCGAGACGG AGGCCTTCTC CCTGAACTCA 
GACAGGTCCT CGTCCATCCT GCTTGGCGAT GACCTGAGTC TGGAGGACCC TGCAGCCTGC 
CCTGTCGGCC CCAGGGACAG CAAGGGCGAG CCTGACCCGC CTGAGCCCAT GCAGGTAGGG 
GACCTGGGCG AATGCTTCTT CCCCTTACCC AACACGGCCG TCTCATCGGG TCCGGAGAAC 
TTCCTGTGCG AGATGGAGGC CATCCCATTC AACCCCGTCC AGTCCTGGCT GAAACACGAG 
AGCAGCCAAG CAGCGCCCCC GAGCCCCTTC TCCCCGGATG CCTCCAGCCC GCTCCTGCCC 
ATGCCGGCCG AGTTCTTCCA CCCTGCCGTG GCCGCCAGCC AGGAAGGGCA GGAGAAGGGC 
GCCGGATCCC GGACCCTCCC CAAGATCGCC CTGCAGGGCT CCTGGGCCTC CCTGAGGTCG 
CCGAGCGTCA ACTGCACCCT CCTTCGACAG GCCACGGGGA GCGACACGTC GCTGGAGGTC 
AGCCGCAGCA GCTCGGCGGG CAGCCTGCAG ACCACGCTGG AGGACAGCCT GACCCTGAGC 
GACAGCCCGC GGCGAGGGCT GGGGCTGCCC GCGTCCGTCC CGGGCCCCCG CGCCGGCCCG 
TCGCCCGCCG CGCGCCGCCG CCCGAGCCCA CGGGGCCGGG GCACGTTCAG CCTGCGCGGG 
CTGCGGGCGC ACCAGCGCAG CCACAGCAGC GGGGGCTCCA CCAGCCCGGG CTGCACGCGG 
CACGACTCCA TGGACCCCTC GGACGAGGAG GGCGCGGGCG GCCGGGCCGG CGGGGGCTGC 
GGGGGCGCGG GCAGCGAGCA CTCGGAGACC CTCAGCAGCC TCTCGCTCAC CTCCCTCTTC 
GGGCCACCGC CCCCGCCGCC CGGCCTCCCG CCCGCGCGCC GGTTCAGCAG CGCCAGCAGC 
CTGGCCGCCG GGCCCGGCCG CCCCCGCGCC GCCGCCGCCC CGGGGCCCCA CGGCCTGGCC 
CGCAGCCCAT CCTGGGCCGC CGCCGATCGC TGCAAGGACC CGCCGCGCCC GGCCGAGCCG 
GCGGCTCCCC GGGACCCCGA GGCGCCTGAG CCACAGCCCC CGCCGCCGCC GCCGGGGGAG 
CCGCCGTCCG GGGACGCCGC GAGCCAGGGG AGGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848685.1
CDS	578..5236
Translation	MFALEMLLKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKLQEGLDCSGDPKLCPIPMTPNGHLDPNLPLGGHLGPTGAAASAPRLSLQPDPVLMALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGAWASRRSSWNSLKHKQPSAEHESLLSAERGGARGCEGARDEGPLRTAPLHAPHAHHAHHGPHPVHRHHHHRRTLSLDTRDSVDLAELVPAVGTHSRAAWRAAGLAPGHEDCNGRMPSIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVPNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELELAHGLGPSPRPPASAPGAPAPAHGPGGAAGEGDPEGRLRRSCYSPAQENLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCEKCHHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPAACPVGPRDSKGEPDPPEPMQVGDLGECFFPLPNTAVSSGPENFLCEMEAIPFNPVQSWLKHESSQAAPPSPFSPDASSPLLPMPAEFFHPAVAASQEGQEKGAGSRTLPKIALQGSWASLRSPSVNCTLLRQATGSDTSLEVSRSSSAGSLQTTLEDSLTLSDSPRRGLGLPASVPGPRAGPSPAARRRPSPRGRGTFSLRGLRAHQRSHSSGGSTSPGCTRHDSMDPSDEEGAGGRAGGGCGGAGSEHSETLSSLSLTSLFGPPPPPPGLPPARRFSSASSLAAGPGRPRAAAAPGPHGLARSPSWAAADRCKDPPRPAEPAAPRDPEAPEPQPPPPPPGEPPSGDAASQGRR

Target ORF information:

RefSeq Version	XM_024992917.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 I (CACNA1I), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992917.1

ORF Insert Sequence:

ATGTTTGCCC TGGAGATGCT GCTGAAGCTG GCCGCCTTCG GGCTCTTCGA CTACCTGCGC 
AACCCCTATA ACATCTTCGA CAGCATCATC GTCATCATCA GCATCTGGGA GATCGTGGGG 
CAGGCGGACG GCGGGCTGTC AGTGCTGCGG ACTTTCCGGC TACTGCGGGT GCTGAAGCTG 
GTGCGCTTTA TGCCCGCGCT GCGGCGCCAG CTCGTGGTGC TCATGAAGAC CATGGACAAC 
GTGGCCACCT TCTGCATGCT GCTCATGCTT TTCATCTTCA TCTTCAGCAT CCTCGGGATG 
CACATCTTTG GCTGCAAATT CAGCCTGCGC ACGGACACGG GAGACACGGT CCCCGACAGG 
AAGAACTTTG ACTCCCTGCT GTGGGCCATC GTCACGGTGT TCCAGATCCT CACCCAGGAG 
GACTGGAACG TCGTCCTCTA CAACGGCATG GCTTCCACAT CCCCCTGGGC CTCCCTCTAT 
TTCGTTGCCC TCATGACCTT TGGCAATTAT GTGCTCTTCA ACCTGCTGGT GGCCATCCTG 
GTGGAGGGCT TCCAGGCGGA GGGTGATGCC AATCGCTCCT ACTCGGACGA GGACCAGAGC 
TCATCCAACA TGGAGGAGTT TGACAAGCTC CAGGAGGGCC TGGACTGCAG CGGAGATCCC 
AAGCTCTGCC CAATCCCCAT GACCCCCAAT GGGCACCTGG ACCCCAATCT CCCACTGGGT 
GGACATCTGG GCCCCACTGG GGCTGCAGCA TCTGCCCCCC GCCTCTCACT GCAGCCAGAC 
CCCGTGCTAA TGGCCCTGGG CTCCCGCAAG AGCAGTGTCA TGTCACTGGG GAGGATGAGC 
TACGACCAGC GCTCCCTGTC CAGCTCTCGG AGCTCCTACT ATGGGCCGTG GGGCCGCAGC 
GGGGCCTGGG CCAGCCGTCG CTCCAGCTGG AACAGCCTCA AGCACAAGCA ACCGTCGGCG 
GAGCATGAGT CCCTGCTCTC CGCTGAGCGT GGCGGCGCCC GGGGCTGCGA GGGCGCCCGG 
GACGAGGGCC CGCTACGGAC CGCGCCCCTG CACGCCCCGC ACGCCCACCA TGCGCATCAT 
GGTCCCCACC CGGTGCATCG TCACCACCAC CACCGCCGAA CGCTATCCCT CGACACCAGA 
GATTCGGTGG ACCTGGCCGA GCTGGTGCCC GCCGTGGGCA CCCACTCCCG GGCGGCCTGG 
AGGGCGGCAG GCCTGGCCCC CGGGCACGAG GACTGCAATG GCAGGATGCC CAGCATCGCC 
AAGGACGTCT TCACCAAGAT GGATGACCGC CGGGATCGCG GGGAGGACGA GGAGGAGATC 
GACTATACTC TGTGCTTCCG AGTCCGCAAG ATGATTGACG TCTACAAGCC TGACTGGTGT 
GAAGTCCGAG AGGACTGGTC CGTCTACCTC TTCTCCCCCG AGAACAGGTT CCGGGTCCTG 
TGTCAGACCA TCATTGCCCA CAAGCTCTTC GACTATGTCG TCCTGGCCTT CATCTTCCTC 
AACTGCATCA CCATCGCCCT GGAGCGGCCC CAGATTGAGG CTGGCAGCAC CGAACGCATC 
TTCCTCACTG TGTCCAACTA CATCTTCACA GCTATCTTCG TGGGCGAGAT GACACTGAAG 
GTGGTCTCGC TGGGCCTGTA CTTCGGGGAG CAGGCGTACC TGCGCAGCAG CTGGAACGTA 
CTGGACGGCT TCCTCGTCTT CGTGTCAATC ATTGACATTG TGGTGTCCGT GGCCTCGGCT 
GGGGGAGCCA AGATCTTGGG CGTCCTCCGG GTCCTGCGGC TCCTGCGCAC CCTACGACCC 
CTGCGTGTCA TCAGCCGGGC ACCTGGCCTG AAGCTGGTGG TGGAGACACT CATCTCCTCC 
CTAAAGCCCA TCGGCAACAT CGTCCTCATC TGCTGCGCCT TCTTCATCAT CTTCGGCATC 
CTGGGGGTGC AGCTCTTCAA GGGCAAGTTC TACCACTGTC TGGGTGTAGA CACCCGCAAC 
ATCACCAACC GCTCTGACTG CATGGCTGCC AACTACCGCT GGGTCCATCA CAAGTACAAC 
TTCGACAATC TGGGCCAGGC CCTCATGTCC CTCTTCGTGC TGGCCTCCAA GGATGGCTGG 
GTGAACATTA TGTACAATGG ACTGGACGCC GTCGCGGTGG ACCAGCAGCC CGTGCCCAAC 
CACAACCCCT GGATGCTGCT CTACTTCATC TCCTTCCTGC TCATCGTCAG CTTCTTCGTG 
CTCAACATGT TCGTGGGTGT CGTGGTGGAG AACTTCCACA AGTGCCGGCA GCACCAGGAG 
GCCGAGGAGG CGCGGCGGCG CGAGGAGAAG CGGCTGCGGC GGCTGGAGAA GAAGCGCCGG 
AAGGCCCAGC GGCTGCCCTA CTACGCTACC TACTGTCCCA CGAGGCTGCT CATCCACTCC 
ATGTGCACCA GCCACTACCT GGACATCTTC ATCACCTTCA TCATCTGCCT CAACGTGGTC 
ACCATGTCCC TGGAGCACTA CAACCAGCCC ACGTCTCTGG AGACAGCCCT CAAGTACTGC 
AACTACATGT TCACCACTGT CTTTGTGCTG GAGGCTGTGC TGAAGCTGGT GGCATTTGGT 
CTGAGACGCT TCTTCAAGGA CCGGTGGAAC CAACTGGACC TGGCCATCGT GCTGCTGTCG 
GTCATGGGCA TCACGCTGGA GGAGATTGAG ATCAACGCGG CGCTGCCCAT CAACCCCACC 
ATCATCCGAA TCATGCGGGT CCTGCGCATC GCCCGGGTGC TGAAGCTCCT GAAGATGGCC 
ACGGGAATGC GGGCCCTGCT GGACACTGTG GTGCAAGCTC TGCCCCAGGT GGGCAACCTG 
GGCCTCCTCT TCATGCTGCT CTTCTTCATC TACGCCGCCC TGGGAGTGGA GCTCTTCGGG 
AAGCTGGTCT GCAACGACGA GAACCCTTGT GAGGGCATGA GCCGGCACGC CACCTTCGAG 
AACTTCGGCA TGGCCTTCCT CACGCTCTTC CAGGTCTCCA CTGGCGACAA CTGGAATGGG 
ATCATGAAGG ACACACTGCG GGACTGCACG CACGACGAGC GCAGCTGCCT GAGCAGCCTG 
CAGTTCGTGT CACCGCTGTA CTTCGTCAGC TTCGTGCTCA CGGCCCAGTT CGTGCTCATC 
AACGTGGTGG TGGCCGTGCT CATGAAGCAC CTGGACGACA GCAACAAGGA GGCCCAGGAG 
GACGCCGAGA TGGACGCCGA GCTGGAGCTG GAGCTGGCCC ACGGCCTGGG CCCCAGCCCG 
CGGCCACCGG CCAGTGCCCC GGGGGCGCCC GCACCCGCCC ACGGGCCAGG AGGGGCGGCC 
GGCGAGGGTG ATCCCGAGGG CCGCCTGCGC CGAAGCTGCT ACTCGCCAGC CCAGGAGAAC 
CTGTGGCTGG ACAGCGTCTC TTTAATCATC AAGGACTCCT TGGAGGGGGA GCTGACCATC 
ATCGACAACC TGTCCGGGTC CATCTTCCAC CACTACTCCT CGCCGGCCGG CTGCGAGAAG 
TGTCACCACG ATAAGCAGGA GGTGCAGCTG GCCGAGACGG AGGCCTTCTC CCTGAACTCA 
GACAGGTCCT CGTCCATCCT GCTTGGCGAT GACCTGAGTC TGGAGGACCC TGCAGCCTGC 
CCTGTCGGCC CCAGGGACAG CAAGGGCGAG CCTGACCCGC CTGAGCCCAT GCAGGTAGGG 
GACCTGGGCG AATGCTTCTT CCCCTTACCC AACACGGCCG TCTCATCGGG TCCGGAGAAC 
TTCCTGTGCG AGATGGAGGC CATCCCATTC AACCCCGTCC AGTCCTGGCT GAAACACGAG 
AGCAGCCAAG CAGCGCCCCC GAGCCCCTTC TCCCCGGATG CCTCCAGCCC GCTCCTGCCC 
ATGCCGGCCG AGTTCTTCCA CCCTGCCGTG GCCGCCAGCC AGGAAGGGCA GGAGAAGGGC 
GCCGGATCCC GGACCCTCCC CAAGATCGCC CTGCAGGGCT CCTGGGCCTC CCTGAGGTCG 
CCGAGCGTCA ACTGCACCCT CCTTCGACAG GCCACGGGGA GCGACACGTC GCTGGAGGTC 
AGCCGCAGCA GCTCGGCGGG CAGCCTGCAG ACCACGCTGG AGGACAGCCT GACCCTGAGC 
GACAGCCCGC GGCGAGGGCT GGGGCTGCCC GCGTCCGTCC CGGGCCCCCG CGCCGGCCCG 
TCGCCCGCCG CGCGCCGCCG CCCGAGCCCA CGGGGCCGGG GCACGTTCAG CCTGCGCGGG 
CTGCGGGCGC ACCAGCGCAG CCACAGCAGC GGGGGCTCCA CCAGCCCGGG CTGCACGCGG 
CACGACTCCA TGGACCCCTC GGACGAGGAG GGCGCGGGCG GCCGGGCCGG CGGGGGCTGC 
GGGGGCGCGG GCAGCGAGCA CTCGGAGACC CTCAGCAGCC TCTCGCTCAC CTCCCTCTTC 
GGGCCACCGC CCCCGCCGCC CGGCCTCCCG CCCGCGCGCC GGTTCAGCAG CGCCAGCAGC 
CTGGCCGCCG GGCCCGGCCG CCCCCGCGCC GCCGCCGCCC CGGGGCCCCA CGGCCTGGCC 
CGCAGCCCAT CCTGGGCCGC CGCCGATCGC TGCAAGGACC CGCCGCGCCC GGCCGAGCCG 
GCGGCTCCCC GGGACCCCGA GGCGCCTGAG CCACAGCCCC CGCCGCCGCC GCCGGGGGAG 
CCGCCGTCCG GGGACGCCGC GAGCCAGGGG AGGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_015463606.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319092.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa90014
Clone ID Related Accession (Same CDS sequence)	XM_015463606.1
Accession Version	XM_015463606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGAGA ACCTGTGGCT GGACAGCGTC 
TCTTTAATCA TCAAGGACTC CTTGGAGGGG GAGCTGACCA TCATCGACAA CCTGTCCGGG 
TCCATCTTCC ACCACTACTC CTCGCCGGCC GGCTGCGAGA AGTGTCACCA CGATAAGCAG 
GAGGTGCAGC TGGCCGAGAC GGAGGCCTTC TCCCTGAACT CAGACAGGTC CTCGTCCATC 
CTGCTTGGCG ATGACCTGAG TCTGGAGGAC CCTGCAGCCT GCCCTGTCGG CCCCAGGGAC 
AGCAAGGGCG AGCCTGACCC GCCTGAGCCC ATGCAGGTAG GGGACCTGGG CGAATGCTTC 
TTCCCCTTAC CCAACACGGC CGTCTCATCG GGTCCGGAGA ACTTCCTGTG CGAGATGGAG 
GCCATCCCAT TCAACCCCGT CCAGTCCTGG CTGAAACACG AGAGCAGCCA AGCAGCGCCC 
CCGAGCCCCT TCTCCCCGGA TGCCTCCAGC CCGCTCCTGC CCATGCCGGC CGAGTTCTTC 
CACCCTGCCG TGGCCGCCAG CCAGGAAGGG CAGGAGAAGG GCGCCGGATC CCGGACCCTC 
CCCAAGATCG CCCTGCAGGG CTCCTGGGCC TCCCTGAGGT CGCCGAGCGT CAACTGCACC 
CTCCTTCGAC AGGCCACGGG GAGCGACACG TCGCTGGAGG TCAGCCGCAG CAGCTCGGCG 
GCAGCCTGCA GACCACGCTG GAGGACAGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319092.1
CDS	346..6618
Translation	MFFRPVVDTWWAEVPGSLCKARGYFSRTKSKALSSGENSASLTSRAKRQEVPPPACCPQEPEKCPNVSALGGPADSLAATGSSWGSLLSADMAENSPPPPSPTAARVAEPGVTEQPGPQSPPPSPPGPEEPLDGVDPEVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECISMLVILLNCVTLGMYQPCDDMDCLSERCKILQVFDDFIFVFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALRSRRQAAGMPGSATPGPRAKEPRHYKLCPRHSPLDPTPHTLVQPISAMLASDPASCPRCQHESDRRPSGQGSSSDSGQEGSGSGDAVGSEDEVGADVARTSEDGASLELGKEDEEGPADGAARLCEDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHQQPEELTNILEICNVVFTSMFALEMLLKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKLQEGLDCSGDPKLCPIPMTPNGHLDPNLPLGGHLGPTGAAASAPRLSLQPDPVLMALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGAWASRRSSWNSLKHKQPSAEHESLLSAERGGARGCEGARDEGPLRTAPLHAPHAHHAHHGPHPVHRHHHHRRTLSLDTRDSVDLAELVPAVGTHSRAAWRAAGLAPGHEDCNGRMPSIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVPNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELELAHGLGPSPRPPASAPGAPAPAHGPGGAAGEGDPEGRLRRSCYSPAQENLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCEKCHHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPAACPVGPRDSKGEPDPPEPMQVGDLGECFFPLPNTAVSSGPENFLCEMEAIPFNPVQSWLKHESSQAAPPSPFSPDASSPLLPMPAEFFHPAVAASQEGQEKGAGSRTLPKIALQGSWASLRSPSVNCTLLRQATGSDTSLEVSRSSSAAACRPRWRTA

Target ORF information:

RefSeq Version	XM_015463606.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463606.1

ORF Insert Sequence:

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGAGA ACCTGTGGCT GGACAGCGTC 
TCTTTAATCA TCAAGGACTC CTTGGAGGGG GAGCTGACCA TCATCGACAA CCTGTCCGGG 
TCCATCTTCC ACCACTACTC CTCGCCGGCC GGCTGCGAGA AGTGTCACCA CGATAAGCAG 
GAGGTGCAGC TGGCCGAGAC GGAGGCCTTC TCCCTGAACT CAGACAGGTC CTCGTCCATC 
CTGCTTGGCG ATGACCTGAG TCTGGAGGAC CCTGCAGCCT GCCCTGTCGG CCCCAGGGAC 
AGCAAGGGCG AGCCTGACCC GCCTGAGCCC ATGCAGGTAG GGGACCTGGG CGAATGCTTC 
TTCCCCTTAC CCAACACGGC CGTCTCATCG GGTCCGGAGA ACTTCCTGTG CGAGATGGAG 
GCCATCCCAT TCAACCCCGT CCAGTCCTGG CTGAAACACG AGAGCAGCCA AGCAGCGCCC 
CCGAGCCCCT TCTCCCCGGA TGCCTCCAGC CCGCTCCTGC CCATGCCGGC CGAGTTCTTC 
CACCCTGCCG TGGCCGCCAG CCAGGAAGGG CAGGAGAAGG GCGCCGGATC CCGGACCCTC 
CCCAAGATCG CCCTGCAGGG CTCCTGGGCC TCCCTGAGGT CGCCGAGCGT CAACTGCACC 
CTCCTTCGAC AGGCCACGGG GAGCGACACG TCGCTGGAGG TCAGCCGCAG CAGCTCGGCG 
GCAGCCTGCA GACCACGCTG GAGGACAGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_015463607.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319093.1 Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X5, mRNA.

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CloneID	OBa90015
Clone ID Related Accession (Same CDS sequence)	XM_015463607.1
Accession Version	XM_015463607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGACT CCTTGGAGGG GGAGCTGACC 
ATCATCGACA ACCTGTCCGG GTCCATCTTC CACCACTACT CCTCGCCGGC CGGCTGCGAG 
AAGTGTCACC ACGATAAGCA GGAGGTGCAG CTGGCCGAGA CGGAGGCCTT CTCCCTGAAC 
TCAGACAGGT CCTCGTCCAT CCTGCTTGGC GATGACCTGA GTCTGGAGGA CCCTGCAGCC 
TGCCCTGTCG GCCCCAGGGA CAGCAAGGGC GAGCCTGACC CGCCTGAGCC CATGCAGGTA 
GGGGACCTGG GCGAATGCTT CTTCCCCTTA CCCAACACGG CCGTCTCATC GGGTCCGGAG 
AACTTCCTGT GCGAGATGGA GGCCATCCCA TTCAACCCCG TCCAGTCCTG GCTGAAACAC 
GAGAGCAGCC AAGCAGCGCC CCCGAGCCCC TTCTCCCCGG ATGCCTCCAG CCCGCTCCTG 
CCCATGCCGG CCGAGTTCTT CCACCCTGCC GTGGCCGCCA GCCAGGAAGG GCAGGAGAAG 
GGCGCCGGAT CCCGGACCCT CCCCAAGATC GCCCTGCAGG GCTCCTGGGC CTCCCTGAGG 
TCGCCGAGCG TCAACTGCAC CCTCCTTCGA CAGGCCACGG GGAGCGACAC GTCGCTGGAG 
GTCAGCCGCA GCAGCTCGGC GGCAGCCTGC AGACCACGCT GGAGGACAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319093.1
CDS	347..6580
Translation	MFFRPVVDTWWAEVPGSLCKARGYFSRTKSKALSSGENSASLTSRAKRQEVPPPACCPQEPEKCPNVSALGGPADSLAATGSSWGSLLSADMAENSPPPPSPTAARVAEPGVTEQPGPQSPPPSPPGPEEPLDGVDPEVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECISMLVILLNCVTLGMYQPCDDMDCLSERCKILQVFDDFIFVFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALRSRRQAAGMPGSATPGPRAKEPRHYKLCPRHSPLDPTPHTLVQPISAMLASDPASCPRCQHESDRRPSGQGSSSDSGQEGSGSGDAVGSEDEVGADVARTSEDGASLELGKEDEEGPADGAARLCEDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHQQPEELTNILEICNVVFTSMFALEMLLKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKLQEGLDCSGDPKLCPIPMTPNGHLDPNLPLGGHLGPTGAAASAPRLSLQPDPVLMALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGAWASRRSSWNSLKHKQPSAEHESLLSAERGGARGCEGARDEGPLRTAPLHAPHAHHAHHGPHPVHRHHHHRRTLSLDTRDSVDLAELVPAVGTHSRAAWRAAGLAPGHEDCNGRMPSIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVPNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELELAHGLGPSPRPPASAPGAPAPAHGPGGAAGEGDPEGRLRRSCYSPAQDSLEGELTIIDNLSGSIFHHYSSPAGCEKCHHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPAACPVGPRDSKGEPDPPEPMQVGDLGECFFPLPNTAVSSGPENFLCEMEAIPFNPVQSWLKHESSQAAPPSPFSPDASSPLLPMPAEFFHPAVAASQEGQEKGAGSRTLPKIALQGSWASLRSPSVNCTLLRQATGSDTSLEVSRSSSAAACRPRWRTA

Target ORF information:

RefSeq Version	XM_015463607.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463607.1

ORF Insert Sequence:

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGACT CCTTGGAGGG GGAGCTGACC 
ATCATCGACA ACCTGTCCGG GTCCATCTTC CACCACTACT CCTCGCCGGC CGGCTGCGAG 
AAGTGTCACC ACGATAAGCA GGAGGTGCAG CTGGCCGAGA CGGAGGCCTT CTCCCTGAAC 
TCAGACAGGT CCTCGTCCAT CCTGCTTGGC GATGACCTGA GTCTGGAGGA CCCTGCAGCC 
TGCCCTGTCG GCCCCAGGGA CAGCAAGGGC GAGCCTGACC CGCCTGAGCC CATGCAGGTA 
GGGGACCTGG GCGAATGCTT CTTCCCCTTA CCCAACACGG CCGTCTCATC GGGTCCGGAG 
AACTTCCTGT GCGAGATGGA GGCCATCCCA TTCAACCCCG TCCAGTCCTG GCTGAAACAC 
GAGAGCAGCC AAGCAGCGCC CCCGAGCCCC TTCTCCCCGG ATGCCTCCAG CCCGCTCCTG 
CCCATGCCGG CCGAGTTCTT CCACCCTGCC GTGGCCGCCA GCCAGGAAGG GCAGGAGAAG 
GGCGCCGGAT CCCGGACCCT CCCCAAGATC GCCCTGCAGG GCTCCTGGGC CTCCCTGAGG 
TCGCCGAGCG TCAACTGCAC CCTCCTTCGA CAGGCCACGG GGAGCGACAC GTCGCTGGAG 
GTCAGCCGCA GCAGCTCGGC GGCAGCCTGC AGACCACGCT GGAGGACAGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_010805738.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804040.2 Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X1, mRNA.

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CloneID	OBa90823
Clone ID Related Accession (Same CDS sequence)	XM_010805738.2
Accession Version	XM_010805738.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805738.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGAGA ACCTGTGGCT GGACAGCGTC 
TCTTTAATCA TCAAGGACTC CTTGGAGGGG GAGCTGACCA TCATCGACAA CCTGTCCGGG 
TCCATCTTCC ACCACTACTC CTCGCCGGCC GGCTGCGAGA AGTGTCACCA CGATAAGCAG 
GAGGTGCAGC TGGCCGAGAC GGAGGCCTTC TCCCTGAACT CAGACAGGTC CTCGTCCATC 
CTGCTTGGCG ATGACCTGAG TCTGGAGGAC CCTGCAGCCT GCCCTGTCGG CCCCAGGGAC 
AGCAAGGGCG AGCCTGACCC GCCTGAGCCC ATGCAGGTAG GGGACCTGGG CGAATGCTTC 
TTCCCCTTAC CCAACACGGC CGTCTCATCG GGTCCGGAGA ACTTCCTGTG CGAGATGGAG 
GCCATCCCAT TCAACCCCGT CCAGTCCTGG CTGAAACACG AGAGCAGCCA AGCAGCGCCC 
CCGAGCCCCT TCTCCCCGGA TGCCTCCAGC CCGCTCCTGC CCATGCCGGC CGAGTTCTTC 
CACCCTGCCG TGGCCGCCAG CCAGGAAGGG CAGGAGAAGG GCGCCGGATC CCGGACCCTC 
CCCAAGATCG CCCTGCAGGG CTCCTGGGCC TCCCTGAGGT CGCCGAGCGT CAACTGCACC 
CTCCTTCGAC AGGTACGGTC CCTCCGGGCT CTGAGAGCTC TCCTGTCAGG GGACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804040.2
CDS	300..6539
Translation	MFFRPVVDTWWAEVPGSLCKARGYFSRTKSKALSSGENSASLTSRAKRQEVPPPACCPQEPEKCPNVSALGGPADSLAATGSSWGSLLSADMAENSPPPPSPTAARVAEPGVTEQPGPQSPPPSPPGPEEPLDGVDPEVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECISMLVILLNCVTLGMYQPCDDMDCLSERCKILQVFDDFIFVFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALRSRRQAAGMPGSATPGPRAKEPRHYKLCPRHSPLDPTPHTLVQPISAMLASDPASCPRCQHESDRRPSGQGSSSDSGQEGSGSGDAVGSEDEVGADVARTSEDGASLELGKEDEEGPADGAARLCEDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHQQPEELTNILEICNVVFTSMFALEMLLKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKLQEGLDCSGDPKLCPIPMTPNGHLDPNLPLGGHLGPTGAAASAPRLSLQPDPVLMALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGAWASRRSSWNSLKHKQPSAEHESLLSAERGGARGCEGARDEGPLRTAPLHAPHAHHAHHGPHPVHRHHHHRRTLSLDTRDSVDLAELVPAVGTHSRAAWRAAGLAPGHEDCNGRMPSIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVPNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELELAHGLGPSPRPPASAPGAPAPAHGPGGAAGEGDPEGRLRRSCYSPAQENLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCEKCHHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPAACPVGPRDSKGEPDPPEPMQVGDLGECFFPLPNTAVSSGPENFLCEMEAIPFNPVQSWLKHESSQAAPPSPFSPDASSPLLPMPAEFFHPAVAASQEGQEKGAGSRTLPKIALQGSWASLRSPSVNCTLLRQVRSLRALRALLSGDM

Target ORF information:

RefSeq Version	XM_010805738.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805738.2

ORF Insert Sequence:

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGAGA ACCTGTGGCT GGACAGCGTC 
TCTTTAATCA TCAAGGACTC CTTGGAGGGG GAGCTGACCA TCATCGACAA CCTGTCCGGG 
TCCATCTTCC ACCACTACTC CTCGCCGGCC GGCTGCGAGA AGTGTCACCA CGATAAGCAG 
GAGGTGCAGC TGGCCGAGAC GGAGGCCTTC TCCCTGAACT CAGACAGGTC CTCGTCCATC 
CTGCTTGGCG ATGACCTGAG TCTGGAGGAC CCTGCAGCCT GCCCTGTCGG CCCCAGGGAC 
AGCAAGGGCG AGCCTGACCC GCCTGAGCCC ATGCAGGTAG GGGACCTGGG CGAATGCTTC 
TTCCCCTTAC CCAACACGGC CGTCTCATCG GGTCCGGAGA ACTTCCTGTG CGAGATGGAG 
GCCATCCCAT TCAACCCCGT CCAGTCCTGG CTGAAACACG AGAGCAGCCA AGCAGCGCCC 
CCGAGCCCCT TCTCCCCGGA TGCCTCCAGC CCGCTCCTGC CCATGCCGGC CGAGTTCTTC 
CACCCTGCCG TGGCCGCCAG CCAGGAAGGG CAGGAGAAGG GCGCCGGATC CCGGACCCTC 
CCCAAGATCG CCCTGCAGGG CTCCTGGGCC TCCCTGAGGT CGCCGAGCGT CAACTGCACC 
CTCCTTCGAC AGGTACGGTC CCTCCGGGCT CTGAGAGCTC TCCTGTCAGG GGACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_010805739.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804041.2 Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X2, mRNA.

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CloneID	OBa90824
Clone ID Related Accession (Same CDS sequence)	XM_010805739.2
Accession Version	XM_010805739.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805739.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGAGA ACCTGTGGCT GGACAGCGTC 
TCTTTAATCA TCAAGGACTC CTTGGAGGGG GAGCTGACCA TCATCGACAA CCTGTCCGGG 
TCCATCTTCC ACCACTACTC CTCGCCGGCC GGCTGCGAGA AGTGTCACCA CGATAAGCAG 
GAGGTGCAGC TGGCCGAGAC GGAGGCCTTC TCCCTGAACT CAGACAGGTC CTCGTCCATC 
CTGCTTGGCG ATGACCTGAG TCTGGAGGAC CCTGCAGCCT GCCCTGTCGG CCCCAGGGAC 
AGCAAGGGCG AGCCTGACCC GCCTGAGCCC ATGCAGGTAG GGGACCTGGG CGAATGCTTC 
TTCCCCTTAC CCAACACGGC CGTCTCATCG GGTCCGGAGA ACTTCCTGTG CGAGATGGAG 
GCCATCCCAT TCAACCCCGT CCAGTCCTGG CTGAAACACG AGAGCAGCCA AGCGCCCCCG 
AGCCCCTTCT CCCCGGATGC CTCCAGCCCG CTCCTGCCCA TGCCGGCCGA GTTCTTCCAC 
CCTGCCGTGG CCGCCAGCCA GGAAGGGCAG GAGAAGGGCG CCGGATCCCG GACCCTCCCC 
AAGATCGCCC TGCAGGGCTC CTGGGCCTCC CTGAGGTCGC CGAGCGTCAA CTGCACCCTC 
CTTCGACAGG TACGGTCCCT CCGGGCTCTG AGAGCTCTCC TGTCAGGGGA CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804041.2
CDS	301..6537
Translation	MFFRPVVDTWWAEVPGSLCKARGYFSRTKSKALSSGENSASLTSRAKRQEVPPPACCPQEPEKCPNVSALGGPADSLAATGSSWGSLLSADMAENSPPPPSPTAARVAEPGVTEQPGPQSPPPSPPGPEEPLDGVDPEVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECISMLVILLNCVTLGMYQPCDDMDCLSERCKILQVFDDFIFVFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALRSRRQAAGMPGSATPGPRAKEPRHYKLCPRHSPLDPTPHTLVQPISAMLASDPASCPRCQHESDRRPSGQGSSSDSGQEGSGSGDAVGSEDEVGADVARTSEDGASLELGKEDEEGPADGAARLCEDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHQQPEELTNILEICNVVFTSMFALEMLLKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKLQEGLDCSGDPKLCPIPMTPNGHLDPNLPLGGHLGPTGAAASAPRLSLQPDPVLMALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGAWASRRSSWNSLKHKQPSAEHESLLSAERGGARGCEGARDEGPLRTAPLHAPHAHHAHHGPHPVHRHHHHRRTLSLDTRDSVDLAELVPAVGTHSRAAWRAAGLAPGHEDCNGRMPSIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVPNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELELAHGLGPSPRPPASAPGAPAPAHGPGGAAGEGDPEGRLRRSCYSPAQENLWLDSVSLIIKDSLEGELTIIDNLSGSIFHHYSSPAGCEKCHHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPAACPVGPRDSKGEPDPPEPMQVGDLGECFFPLPNTAVSSGPENFLCEMEAIPFNPVQSWLKHESSQAPPSPFSPDASSPLLPMPAEFFHPAVAASQEGQEKGAGSRTLPKIALQGSWASLRSPSVNCTLLRQVRSLRALRALLSGDM

Target ORF information:

RefSeq Version	XM_010805739.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805739.2

ORF Insert Sequence:

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGAGA ACCTGTGGCT GGACAGCGTC 
TCTTTAATCA TCAAGGACTC CTTGGAGGGG GAGCTGACCA TCATCGACAA CCTGTCCGGG 
TCCATCTTCC ACCACTACTC CTCGCCGGCC GGCTGCGAGA AGTGTCACCA CGATAAGCAG 
GAGGTGCAGC TGGCCGAGAC GGAGGCCTTC TCCCTGAACT CAGACAGGTC CTCGTCCATC 
CTGCTTGGCG ATGACCTGAG TCTGGAGGAC CCTGCAGCCT GCCCTGTCGG CCCCAGGGAC 
AGCAAGGGCG AGCCTGACCC GCCTGAGCCC ATGCAGGTAG GGGACCTGGG CGAATGCTTC 
TTCCCCTTAC CCAACACGGC CGTCTCATCG GGTCCGGAGA ACTTCCTGTG CGAGATGGAG 
GCCATCCCAT TCAACCCCGT CCAGTCCTGG CTGAAACACG AGAGCAGCCA AGCGCCCCCG 
AGCCCCTTCT CCCCGGATGC CTCCAGCCCG CTCCTGCCCA TGCCGGCCGA GTTCTTCCAC 
CCTGCCGTGG CCGCCAGCCA GGAAGGGCAG GAGAAGGGCG CCGGATCCCG GACCCTCCCC 
AAGATCGCCC TGCAGGGCTC CTGGGCCTCC CTGAGGTCGC CGAGCGTCAA CTGCACCCTC 
CTTCGACAGG TACGGTCCCT CCGGGCTCTG AGAGCTCTCC TGTCAGGGGA CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1I ( XM_010805740.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804042.2 Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X3, mRNA.

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CloneID	OBa90825
Clone ID Related Accession (Same CDS sequence)	XM_010805740.2
Accession Version	XM_010805740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent T-type calcium channel subunit alpha-1I isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGACT CCTTGGAGGG GGAGCTGACC 
ATCATCGACA ACCTGTCCGG GTCCATCTTC CACCACTACT CCTCGCCGGC CGGCTGCGAG 
AAGTGTCACC ACGATAAGCA GGAGGTGCAG CTGGCCGAGA CGGAGGCCTT CTCCCTGAAC 
TCAGACAGGT CCTCGTCCAT CCTGCTTGGC GATGACCTGA GTCTGGAGGA CCCTGCAGCC 
TGCCCTGTCG GCCCCAGGGA CAGCAAGGGC GAGCCTGACC CGCCTGAGCC CATGCAGGTA 
GGGGACCTGG GCGAATGCTT CTTCCCCTTA CCCAACACGG CCGTCTCATC GGGTCCGGAG 
AACTTCCTGT GCGAGATGGA GGCCATCCCA TTCAACCCCG TCCAGTCCTG GCTGAAACAC 
GAGAGCAGCC AAGCAGCGCC CCCGAGCCCC TTCTCCCCGG ATGCCTCCAG CCCGCTCCTG 
CCCATGCCGG CCGAGTTCTT CCACCCTGCC GTGGCCGCCA GCCAGGAAGG GCAGGAGAAG 
GGCGCCGGAT CCCGGACCCT CCCCAAGATC GCCCTGCAGG GCTCCTGGGC CTCCCTGAGG 
TCGCCGAGCG TCAACTGCAC CCTCCTTCGA CAGGTACGGT CCCTCCGGGC TCTGAGAGCT 
CTCCTGTCAG GGGACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804042.2
CDS	301..6501
Translation	MFFRPVVDTWWAEVPGSLCKARGYFSRTKSKALSSGENSASLTSRAKRQEVPPPACCPQEPEKCPNVSALGGPADSLAATGSSWGSLLSADMAENSPPPPSPTAARVAEPGVTEQPGPQSPPPSPPGPEEPLDGVDPEVPHPDLAPVAFFCLRQTTSPRNWCIKMVCNPWFECISMLVILLNCVTLGMYQPCDDMDCLSERCKILQVFDDFIFVFFAMEMVLKMVALGIFGKKCYLGDTWNRLDFFIVMAGMVEYSLDLQNINLSAIRTVRVLRPLKAINRVPSMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCFLEENFTIQGDVALPPYYQPEEDDEMPFICSLSGDNGIMGCHEIPPLKEQGRECCLSKDDVYDFGAGRQDLNASGLCVNWNRYYNVCRTGSANPHKGAINFDNIGYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQREHRLMLEQRQRYLSSSTVASYAEPGDCYEEIFQYVCHILRKAKRRALGLYQALRSRRQAAGMPGSATPGPRAKEPRHYKLCPRHSPLDPTPHTLVQPISAMLASDPASCPRCQHESDRRPSGQGSSSDSGQEGSGSGDAVGSEDEVGADVARTSEDGASLELGKEDEEGPADGAARLCEDVWRETRAKLRGIVDSKYFNRGIMMAILVNTVSMGIEHHQQPEELTNILEICNVVFTSMFALEMLLKLAAFGLFDYLRNPYNIFDSIIVIISIWEIVGQADGGLSVLRTFRLLRVLKLVRFMPALRRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHIFGCKFSLRTDTGDTVPDRKNFDSLLWAIVTVFQILTQEDWNVVLYNGMASTSPWASLYFVALMTFGNYVLFNLLVAILVEGFQAEGDANRSYSDEDQSSSNMEEFDKLQEGLDCSGDPKLCPIPMTPNGHLDPNLPLGGHLGPTGAAASAPRLSLQPDPVLMALGSRKSSVMSLGRMSYDQRSLSSSRSSYYGPWGRSGAWASRRSSWNSLKHKQPSAEHESLLSAERGGARGCEGARDEGPLRTAPLHAPHAHHAHHGPHPVHRHHHHRRTLSLDTRDSVDLAELVPAVGTHSRAAWRAAGLAPGHEDCNGRMPSIAKDVFTKMDDRRDRGEDEEEIDYTLCFRVRKMIDVYKPDWCEVREDWSVYLFSPENRFRVLCQTIIAHKLFDYVVLAFIFLNCITIALERPQIEAGSTERIFLTVSNYIFTAIFVGEMTLKVVSLGLYFGEQAYLRSSWNVLDGFLVFVSIIDIVVSVASAGGAKILGVLRVLRLLRTLRPLRVISRAPGLKLVVETLISSLKPIGNIVLICCAFFIIFGILGVQLFKGKFYHCLGVDTRNITNRSDCMAANYRWVHHKYNFDNLGQALMSLFVLASKDGWVNIMYNGLDAVAVDQQPVPNHNPWMLLYFISFLLIVSFFVLNMFVGVVVENFHKCRQHQEAEEARRREEKRLRRLEKKRRKAQRLPYYATYCPTRLLIHSMCTSHYLDIFITFIICLNVVTMSLEHYNQPTSLETALKYCNYMFTTVFVLEAVLKLVAFGLRRFFKDRWNQLDLAIVLLSVMGITLEEIEINAALPINPTIIRIMRVLRIARVLKLLKMATGMRALLDTVVQALPQVGNLGLLFMLLFFIYAALGVELFGKLVCNDENPCEGMSRHATFENFGMAFLTLFQVSTGDNWNGIMKDTLRDCTHDERSCLSSLQFVSPLYFVSFVLTAQFVLINVVVAVLMKHLDDSNKEAQEDAEMDAELELELAHGLGPSPRPPASAPGAPAPAHGPGGAAGEGDPEGRLRRSCYSPAQDSLEGELTIIDNLSGSIFHHYSSPAGCEKCHHDKQEVQLAETEAFSLNSDRSSSILLGDDLSLEDPAACPVGPRDSKGEPDPPEPMQVGDLGECFFPLPNTAVSSGPENFLCEMEAIPFNPVQSWLKHESSQAAPPSPFSPDASSPLLPMPAEFFHPAVAASQEGQEKGAGSRTLPKIALQGSWASLRSPSVNCTLLRQVRSLRALRALLSGDM

Target ORF information:

RefSeq Version	XM_010805740.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, T type, alpha 1I subunit (CACNA1I), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805740.2

ORF Insert Sequence:

ATGTTTTTCC GCCCAGTGGT TGACACGTGG TGGGCCGAGG TGCCTGGGAG CTTATGTAAG 
GCCCGTGGCT ATTTCTCTAG AACCAAGAGC AAAGCTCTGA GCTCAGGAGA AAACTCGGCC 
TCACTGACCA GCCGGGCGAA GAGGCAGGAG GTGCCACCTC CCGCCTGTTG CCCCCAAGAG 
CCAGAGAAAT GTCCAAATGT ATCAGCTCTG GGTGGCCCAG CTGACAGCTT AGCAGCCACA 
GGCAGCAGCT GGGGCTCCCT GCTCAGCGCC GACATGGCTG AGAACTCCCC ACCGCCCCCT 
TCCCCCACCG CAGCCCGAGT GGCTGAACCC GGAGTCACAG AGCAACCGGG GCCCCAGAGC 
CCCCCTCCCT CTCCTCCTGG CCCAGAAGAA CCCCTGGACG GAGTTGACCC AGAAGTCCCT 
CACCCGGACC TGGCCCCCGT TGCCTTCTTC TGCCTGCGAC AGACCACCAG TCCCCGGAAC 
TGGTGCATCA AGATGGTGTG TAACCCGTGG TTTGAATGTA TCAGCATGCT GGTGATCCTG 
CTGAACTGCG TGACCCTCGG CATGTACCAG CCGTGCGACG ACATGGACTG CCTGTCAGAG 
CGCTGCAAGA TCCTGCAGGT CTTCGATGAC TTCATCTTTG TCTTCTTTGC CATGGAGATG 
GTGCTCAAGA TGGTGGCCCT GGGCATTTTT GGCAAGAAGT GCTACCTTGG GGACACATGG 
AACCGACTGG ATTTCTTCAT TGTCATGGCA GGGATGGTCG AGTACTCCCT GGACCTCCAG 
AACATCAACC TGTCGGCCAT CCGTACCGTG CGCGTCCTGA GGCCCCTCAA AGCTATCAAC 
CGTGTGCCCA GTATGCGGAT CCTGGTGAAC CTGCTCCTGG ACACACTGCC CATGCTGGGG 
AATGTCCTCC TGCTCTGCTT CTTTGTCTTC TTCATCTTTG GCATCATCGG CGTGCAGCTC 
TGGGCCGGCC TGCTTCGTAA CCGCTGCTTC CTGGAGGAGA ACTTCACTAT ACAAGGGGAC 
GTGGCTCTGC CCCCCTACTA CCAGCCGGAG GAGGACGATG AGATGCCCTT CATCTGCTCC 
CTGTCGGGGG ACAACGGCAT CATGGGCTGC CACGAGATCC CTCCGCTCAA GGAGCAGGGC 
CGCGAGTGCT GCCTGTCCAA GGACGACGTC TACGACTTCG GGGCGGGCCG CCAGGACCTC 
AACGCCAGTG GGCTCTGCGT CAACTGGAAC CGCTACTACA ACGTGTGCCG CACGGGCAGC 
GCCAACCCCC ACAAGGGCGC CATCAACTTT GACAACATCG GCTATGCCTG GATCGTCATT 
TTCCAGGTGA TCACTCTGGA AGGCTGGGTG GAGATCATGT ACTATGTGAT GGATGCTCAC 
TCCTTCTACA ACTTCATCTA TTTTATCCTG CTCATCATAG TGGGCTCCTT CTTCATGATC 
AACCTGTGCC TCGTTGTCAT AGCGACCCAG TTCTCGGAGA CCAAGCAGCG GGAGCATCGG 
CTGATGCTGG AGCAGCGGCA GCGCTACCTG TCCTCCAGCA CGGTGGCCAG CTACGCCGAG 
CCCGGCGACT GCTACGAGGA GATCTTCCAG TACGTCTGCC ACATCCTGCG CAAGGCCAAG 
CGCCGGGCCC TGGGCCTCTA CCAGGCCCTG CGGAGCCGGC GCCAGGCCGC GGGGATGCCC 
GGCTCTGCCA CGCCCGGGCC CCGCGCCAAG GAGCCCAGGC ACTACAAGTT ATGCCCCCGA 
CACAGCCCCC TGGACCCGAC ACCCCACACG CTGGTGCAGC CCATCTCTGC CATGCTGGCC 
TCCGACCCTG CCAGCTGCCC CCGCTGCCAG CACGAGAGCG ACCGGCGGCC CTCAGGCCAG 
GGCAGCAGCA GTGACTCGGG CCAGGAGGGC TCGGGCTCCG GGGACGCAGT GGGCAGCGAG 
GATGAAGTCG GCGCGGATGT GGCCCGGACC AGCGAGGACG GGGCCTCCTT GGAGCTGGGG 
AAGGAGGACG AGGAGGGGCC GGCCGACGGG GCGGCTCGGC TGTGCGAAGA CGTGTGGCGG 
GAGACGCGAG CCAAGCTGCG GGGCATCGTG GACAGCAAAT ACTTCAACCG GGGCATCATG 
ATGGCTATTC TGGTCAACAC TGTCAGCATG GGCATCGAGC ACCATCAGCA GCCCGAGGAG 
CTGACCAACA TCCTGGAGAT CTGCAATGTG GTCTTCACCA GCATGTTTGC CCTGGAGATG 
CTGCTGAAGC TGGCCGCCTT CGGGCTCTTC GACTACCTGC GCAACCCCTA TAACATCTTC 
GACAGCATCA TCGTCATCAT CAGCATCTGG GAGATCGTGG GGCAGGCGGA CGGCGGGCTG 
TCAGTGCTGC GGACTTTCCG GCTACTGCGG GTGCTGAAGC TGGTGCGCTT TATGCCCGCG 
CTGCGGCGCC AGCTCGTGGT GCTCATGAAG ACCATGGACA ACGTGGCCAC CTTCTGCATG 
CTGCTCATGC TTTTCATCTT CATCTTCAGC ATCCTCGGGA TGCACATCTT TGGCTGCAAA 
TTCAGCCTGC GCACGGACAC GGGAGACACG GTCCCCGACA GGAAGAACTT TGACTCCCTG 
CTGTGGGCCA TCGTCACGGT GTTCCAGATC CTCACCCAGG AGGACTGGAA CGTCGTCCTC 
TACAACGGCA TGGCTTCCAC ATCCCCCTGG GCCTCCCTCT ATTTCGTTGC CCTCATGACC 
TTTGGCAATT ATGTGCTCTT CAACCTGCTG GTGGCCATCC TGGTGGAGGG CTTCCAGGCG 
GAGGGTGATG CCAATCGCTC CTACTCGGAC GAGGACCAGA GCTCATCCAA CATGGAGGAG 
TTTGACAAGC TCCAGGAGGG CCTGGACTGC AGCGGAGATC CCAAGCTCTG CCCAATCCCC 
ATGACCCCCA ATGGGCACCT GGACCCCAAT CTCCCACTGG GTGGACATCT GGGCCCCACT 
GGGGCTGCAG CATCTGCCCC CCGCCTCTCA CTGCAGCCAG ACCCCGTGCT AATGGCCCTG 
GGCTCCCGCA AGAGCAGTGT CATGTCACTG GGGAGGATGA GCTACGACCA GCGCTCCCTG 
TCCAGCTCTC GGAGCTCCTA CTATGGGCCG TGGGGCCGCA GCGGGGCCTG GGCCAGCCGT 
CGCTCCAGCT GGAACAGCCT CAAGCACAAG CAACCGTCGG CGGAGCATGA GTCCCTGCTC 
TCCGCTGAGC GTGGCGGCGC CCGGGGCTGC GAGGGCGCCC GGGACGAGGG CCCGCTACGG 
ACCGCGCCCC TGCACGCCCC GCACGCCCAC CATGCGCATC ATGGTCCCCA CCCGGTGCAT 
CGTCACCACC ACCACCGCCG AACGCTATCC CTCGACACCA GAGATTCGGT GGACCTGGCC 
GAGCTGGTGC CCGCCGTGGG CACCCACTCC CGGGCGGCCT GGAGGGCGGC AGGCCTGGCC 
CCCGGGCACG AGGACTGCAA TGGCAGGATG CCCAGCATCG CCAAGGACGT CTTCACCAAG 
ATGGATGACC GCCGGGATCG CGGGGAGGAC GAGGAGGAGA TCGACTATAC TCTGTGCTTC 
CGAGTCCGCA AGATGATTGA CGTCTACAAG CCTGACTGGT GTGAAGTCCG AGAGGACTGG 
TCCGTCTACC TCTTCTCCCC CGAGAACAGG TTCCGGGTCC TGTGTCAGAC CATCATTGCC 
CACAAGCTCT TCGACTATGT CGTCCTGGCC TTCATCTTCC TCAACTGCAT CACCATCGCC 
CTGGAGCGGC CCCAGATTGA GGCTGGCAGC ACCGAACGCA TCTTCCTCAC TGTGTCCAAC 
TACATCTTCA CAGCTATCTT CGTGGGCGAG ATGACACTGA AGGTGGTCTC GCTGGGCCTG 
TACTTCGGGG AGCAGGCGTA CCTGCGCAGC AGCTGGAACG TACTGGACGG CTTCCTCGTC 
TTCGTGTCAA TCATTGACAT TGTGGTGTCC GTGGCCTCGG CTGGGGGAGC CAAGATCTTG 
GGCGTCCTCC GGGTCCTGCG GCTCCTGCGC ACCCTACGAC CCCTGCGTGT CATCAGCCGG 
GCACCTGGCC TGAAGCTGGT GGTGGAGACA CTCATCTCCT CCCTAAAGCC CATCGGCAAC 
ATCGTCCTCA TCTGCTGCGC CTTCTTCATC ATCTTCGGCA TCCTGGGGGT GCAGCTCTTC 
AAGGGCAAGT TCTACCACTG TCTGGGTGTA GACACCCGCA ACATCACCAA CCGCTCTGAC 
TGCATGGCTG CCAACTACCG CTGGGTCCAT CACAAGTACA ACTTCGACAA TCTGGGCCAG 
GCCCTCATGT CCCTCTTCGT GCTGGCCTCC AAGGATGGCT GGGTGAACAT TATGTACAAT 
GGACTGGACG CCGTCGCGGT GGACCAGCAG CCCGTGCCCA ACCACAACCC CTGGATGCTG 
CTCTACTTCA TCTCCTTCCT GCTCATCGTC AGCTTCTTCG TGCTCAACAT GTTCGTGGGT 
GTCGTGGTGG AGAACTTCCA CAAGTGCCGG CAGCACCAGG AGGCCGAGGA GGCGCGGCGG 
CGCGAGGAGA AGCGGCTGCG GCGGCTGGAG AAGAAGCGCC GGAAGGCCCA GCGGCTGCCC 
TACTACGCTA CCTACTGTCC CACGAGGCTG CTCATCCACT CCATGTGCAC CAGCCACTAC 
CTGGACATCT TCATCACCTT CATCATCTGC CTCAACGTGG TCACCATGTC CCTGGAGCAC 
TACAACCAGC CCACGTCTCT GGAGACAGCC CTCAAGTACT GCAACTACAT GTTCACCACT 
GTCTTTGTGC TGGAGGCTGT GCTGAAGCTG GTGGCATTTG GTCTGAGACG CTTCTTCAAG 
GACCGGTGGA ACCAACTGGA CCTGGCCATC GTGCTGCTGT CGGTCATGGG CATCACGCTG 
GAGGAGATTG AGATCAACGC GGCGCTGCCC ATCAACCCCA CCATCATCCG AATCATGCGG 
GTCCTGCGCA TCGCCCGGGT GCTGAAGCTC CTGAAGATGG CCACGGGAAT GCGGGCCCTG 
CTGGACACTG TGGTGCAAGC TCTGCCCCAG GTGGGCAACC TGGGCCTCCT CTTCATGCTG 
CTCTTCTTCA TCTACGCCGC CCTGGGAGTG GAGCTCTTCG GGAAGCTGGT CTGCAACGAC 
GAGAACCCTT GTGAGGGCAT GAGCCGGCAC GCCACCTTCG AGAACTTCGG CATGGCCTTC 
CTCACGCTCT TCCAGGTCTC CACTGGCGAC AACTGGAATG GGATCATGAA GGACACACTG 
CGGGACTGCA CGCACGACGA GCGCAGCTGC CTGAGCAGCC TGCAGTTCGT GTCACCGCTG 
TACTTCGTCA GCTTCGTGCT CACGGCCCAG TTCGTGCTCA TCAACGTGGT GGTGGCCGTG 
CTCATGAAGC ACCTGGACGA CAGCAACAAG GAGGCCCAGG AGGACGCCGA GATGGACGCC 
GAGCTGGAGC TGGAGCTGGC CCACGGCCTG GGCCCCAGCC CGCGGCCACC GGCCAGTGCC 
CCGGGGGCGC CCGCACCCGC CCACGGGCCA GGAGGGGCGG CCGGCGAGGG TGATCCCGAG 
GGCCGCCTGC GCCGAAGCTG CTACTCGCCA GCCCAGGACT CCTTGGAGGG GGAGCTGACC 
ATCATCGACA ACCTGTCCGG GTCCATCTTC CACCACTACT CCTCGCCGGC CGGCTGCGAG 
AAGTGTCACC ACGATAAGCA GGAGGTGCAG CTGGCCGAGA CGGAGGCCTT CTCCCTGAAC 
TCAGACAGGT CCTCGTCCAT CCTGCTTGGC GATGACCTGA GTCTGGAGGA CCCTGCAGCC 
TGCCCTGTCG GCCCCAGGGA CAGCAAGGGC GAGCCTGACC CGCCTGAGCC CATGCAGGTA 
GGGGACCTGG GCGAATGCTT CTTCCCCTTA CCCAACACGG CCGTCTCATC GGGTCCGGAG 
AACTTCCTGT GCGAGATGGA GGCCATCCCA TTCAACCCCG TCCAGTCCTG GCTGAAACAC 
GAGAGCAGCC AAGCAGCGCC CCCGAGCCCC TTCTCCCCGG ATGCCTCCAG CCCGCTCCTG 
CCCATGCCGG CCGAGTTCTT CCACCCTGCC GTGGCCGCCA GCCAGGAAGG GCAGGAGAAG 
GGCGCCGGAT CCCGGACCCT CCCCAAGATC GCCCTGCAGG GCTCCTGGGC CTCCCTGAGG 
TCGCCGAGCG TCAACTGCAC CCTCCTTCGA CAGGTACGGT CCCTCCGGGC TCTGAGAGCT 
CTCCTGTCAG GGGACATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CACNA1I cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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