PRKCB cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKCB
Entrez Gene ID	282325
Full Name	protein kinase C beta
Synonyms	PRKCB1
General protein information	Preferred Names protein kinase C beta Names protein kinase C beta type PKC-B PKC-beta protein kinase C, beta 1 polypeptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024984661.1	XP_024840429.1	protein kinase C beta type isoform X2
XM_005224690.3	XP_005224747.1	protein kinase C beta type isoform X1
XM_015460312.1	XP_015315798.1	protein kinase C beta type isoform X2
XM_005224690.4	XP_005224747.1	protein kinase C beta type isoform X1
XM_024984662.1	XP_024840430.1	protein kinase C beta type isoform X4
XM_015460312.2	XP_015315798.1	protein kinase C beta type isoform X3
NM_174587.1	NP_777012.1	protein kinase C beta type

Pathways from BioSystems

Homologies

	PRKCB	XP_001096447.1
Canis lupus familiaris (dog)	PRKCB	XP_005622125.1
Bos taurus (cattle)	PRKCB	NP_777012.1
Rattus norvegicus (Norway rat)	Prkcb	NP_036845.3
Danio rerio (zebrafish)	prkcbb	NP_957272.1
Schizosaccharomyces pombe (fission yeast)	pck1	NP_593737.2
Gallus gallus (chicken)	PRKCB	XP_414868.4
Homo sapiens (human)	PRKCB	NP_002729.2
Mus musculus (house mouse)	Prkcb	NP_032881.1
Xenopus tropicalis (tropical clawed frog)	prkcb	NP_001103513.1
Pan troglodytes (chimpanzee)	PRKCB	XP_003315067.1

Gene Ontology
Bibliography

Related articles in PubMed

Serine phosphorylation sites on IRS2 activated by angiotensin II and protein kinase C to induce selective insulin resistance in endothelial cells.
Park K, Li Q, Rask-Madsen C, Mima A, Mizutani K, Winnay J, Maeda Y, D'Aquino K, White MF, Feener EP, King GL
Molecular and cellular biology33(16)3227-41(2013 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
Nishizuka Y
Nature334(6184)661-5(1988 Aug)

Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways.
Coussens L, Parker PJ, Rhee L, Yang-Feng TL, Chen E, Waterfield MD, Francke U, Ullrich A
Science (New York, N.Y.)233(4766)859-66(1986 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study identified the serine phosphorylation (p-Ser) sites induced by PKC-Beta activation or AGT, which inhibits insulin-induced p-Tyr sites on IRS2 and its signals in endothelial cells.
Title: Serine phosphorylation sites on IRS2 activated by angiotensin II and protein kinase C to induce selective insulin resistance in endothelial cells.

The following PRKCB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKCB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01243	NM_174587.1 Latest version!	Bos taurus protein kinase C beta (PRKCB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa32942	XM_005224690.3 Latest version!	Bos taurus protein kinase C, beta (PRKCB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa230152	XM_005224690.4 Latest version!	Bos taurus protein kinase C beta (PRKCB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa230153	XM_024984661.1 Latest version!	Bos taurus protein kinase C beta (PRKCB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa230154	XM_015460312.2 Latest version!	Bos taurus protein kinase C beta (PRKCB), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa230155	XM_024984662.1 Latest version!	Bos taurus protein kinase C beta (PRKCB), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa88480	XM_015460312.1 Latest version!	Bos taurus protein kinase C, beta (PRKCB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKCB ( NM_174587.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777012.1 Bos taurus protein kinase C beta (PRKCB), mRNA.

Price & Availability↑

CloneID	OBa01243
Clone ID Related Accession (Same CDS sequence)	NM_174587.1
Accession Version	NM_174587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	protein kinase C beta type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M13974.1. ##Evidence-Data-START## Transcript exon combination :: M13974.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGACC CGGCCGCGGG GCCGCCGCCG AGCGAGGGAG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTTCAGTG CCAAGTCTGC TGTTTTGTCG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC GGGCGCTGAC AAGGGCCCAG CCTCTGACGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACATACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
TTGCTCTATG GACTTATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAAGCCCA CATCGAGAGG GAGGTCCTCA TTGTTGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA TCCTAATGGC TTGTCAGATC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGTGA GAGCAAGCAG AAGACCAAGA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAAACATT TAGATTCCAG CTGAAAGAAT CGGACAAAGA CAGGAGACTG 
TCTGTAGAGA TTTGGGATTG GGATCTGACC AGCAGAAATG ACTTCATGGG ATCTTTGTCG 
TTTGGGATTT CTGAACTGCA GAAAGCTGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTGCCCGTG CCACCGGAAG GCAGTGAGGG CAACGAGGAA 
CTGCGACAGA AATTCGAGAG AGCCAAGATC GGTCCTGGGC CCAAGACTCC TGAAGAAAAG 
ACGACCAACA CCATCTCCAA ATTTGACAAC AACGGCAACA GGGACCGGAT GAAATTGACC 
GATTTTAACT TCCTGATGGT GCTGGGAAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGAAAAGGCA CAGATGAGCT GTATGCTGTG AAGATCCTGA AAAAGGACGT GGTCATCCAA 
GATGATGACG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ATCGCCTGTA CTTTGTGATG 
GAGTATGTGA ATGGTGGTGA CCTCATGTAC CACATCCAGC AAGTGGGCCG GTTCAAGGAG 
CCTCACGCCG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC GACTAAGACC 
TTCTGCGGCA CCCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGCGTCCTG CTGTATGAGA TGTTGGCTGG GCAGGCACCG 
TTTGAAGGGG AGGACGAGGA TGAACTCTTC CAGTCCATCA TGGAACACAA CGTGGCTTAT 
CCGAAGTCCA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATCAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ACTGGGAGAA ACTTGAACGC AAAGAGATTC AGCCCCCGTA TAAGCCGAAA 
GCCTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTTCTA 
ACACCTCCTG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCTGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777012.1
CDS	65..2086
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIEREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGPGPKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLITKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS

Target ORF information:

RefSeq Version	NM_174587.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C beta (PRKCB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174587.1

ORF Insert Sequence:

ATGGCTGACC CGGCCGCGGG GCCGCCGCCG AGCGAGGGAG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTTCAGTG CCAAGTCTGC TGTTTTGTCG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC GGGCGCTGAC AAGGGCCCAG CCTCTGACGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACATACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
TTGCTCTATG GACTTATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAAGCCCA CATCGAGAGG GAGGTCCTCA TTGTTGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA TCCTAATGGC TTGTCAGATC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGTGA GAGCAAGCAG AAGACCAAGA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAAACATT TAGATTCCAG CTGAAAGAAT CGGACAAAGA CAGGAGACTG 
TCTGTAGAGA TTTGGGATTG GGATCTGACC AGCAGAAATG ACTTCATGGG ATCTTTGTCG 
TTTGGGATTT CTGAACTGCA GAAAGCTGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTGCCCGTG CCACCGGAAG GCAGTGAGGG CAACGAGGAA 
CTGCGACAGA AATTCGAGAG AGCCAAGATC GGTCCTGGGC CCAAGACTCC TGAAGAAAAG 
ACGACCAACA CCATCTCCAA ATTTGACAAC AACGGCAACA GGGACCGGAT GAAATTGACC 
GATTTTAACT TCCTGATGGT GCTGGGAAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGAAAAGGCA CAGATGAGCT GTATGCTGTG AAGATCCTGA AAAAGGACGT GGTCATCCAA 
GATGATGACG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ATCGCCTGTA CTTTGTGATG 
GAGTATGTGA ATGGTGGTGA CCTCATGTAC CACATCCAGC AAGTGGGCCG GTTCAAGGAG 
CCTCACGCCG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC GACTAAGACC 
TTCTGCGGCA CCCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGCGTCCTG CTGTATGAGA TGTTGGCTGG GCAGGCACCG 
TTTGAAGGGG AGGACGAGGA TGAACTCTTC CAGTCCATCA TGGAACACAA CGTGGCTTAT 
CCGAAGTCCA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATCAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ACTGGGAGAA ACTTGAACGC AAAGAGATTC AGCCCCCGTA TAAGCCGAAA 
GCCTGTGGGC GAAATGCTGA AAACTTCGAC CGATTTTTCA CCCGCCATCC ACCAGTTCTA 
ACACCTCCTG ACCAGGAAGT CATCAGGAAT ATTGACCAAT CAGAATTCGA AGGATTTTCC 
TTTGTTAACT CTGAATTTTT AAAACCTGAA GTCAAGAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( XM_005224690.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224747.1 Bos taurus protein kinase C, beta (PRKCB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32942
Clone ID Related Accession (Same CDS sequence)	XM_005224690.3
Accession Version	XM_005224690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein kinase C beta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CGGCCGCGGG GCCGCCGCCG AGCGAGGGAG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTTCAGTG CCAAGTCTGC TGTTTTGTCG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC GGGCGCTGAC AAGGGCCCAG CCTCTGACGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACATACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
TTGCTCTATG GACTTATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAAGCCCA CATCGAGAGG GAGGTCCTCA TTGTTGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA TCCTAATGGC TTGTCAGATC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGTGA GAGCAAGCAG AAGACCAAGA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAAACATT TAGATTCCAG CTGAAAGAAT CGGACAAAGA CAGGAGACTG 
TCTGTAGAGA TTTGGGATTG GGATCTGACC AGCAGAAATG ACTTCATGGG ATCTTTGTCG 
TTTGGGATTT CTGAACTGCA GAAAGCTGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTGCCCGTG CCACCGGAAG GCAGTGAGGG CAACGAGGAA 
CTGCGACAGA AATTCGAGAG AGCCAAGATC GGTCCTGGGC CCAAGACTCC TGAAGAAAAG 
ACGACCAACA CCATCTCCAA ATTTGACAAC AACGGCAACA GGGACCGGAT GAAATTGACC 
GATTTTAACT TCCTGATGGT GCTGGGAAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGAAAAGGCA CAGATGAGCT GTATGCTGTG AAGATCCTGA AAAAGGACGT GGTCATCCAA 
GATGATGACG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ATCGCCTGTA CTTCGTGATG 
GAGTATGTGA ATGGTGGTGA CCTCATGTAC CACATCCAGC AAGTGGGCCG GTTCAAGGAG 
CCTCACGCCG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC GACTAAGACC 
TTCTGCGGCA CCCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGCGTCCTG CTGTATGAGA TGTTGGCTGG GCAGGCACCG 
TTTGAAGGGG AGGACGAGGA TGAACTCTTC CAGTCCATCA TGGAACACAA CGTGGCTTAT 
CCGAAGTCCA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ACTGGGAGAA ACTTGAACGC AAAGAGATTC AGCCCCCGTA TAAGCCGAAA 
GCCAGAGACA AGCGAGACAC CTCCAATTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTCACGCCTA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCCTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224747.1
CDS	195..2210
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIEREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGPGPKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

Target ORF information:

RefSeq Version	XM_005224690.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C, beta (PRKCB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224690.3

ORF Insert Sequence:

ATGGCTGACC CGGCCGCGGG GCCGCCGCCG AGCGAGGGAG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTTCAGTG CCAAGTCTGC TGTTTTGTCG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC GGGCGCTGAC AAGGGCCCAG CCTCTGACGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACATACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
TTGCTCTATG GACTTATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAAGCCCA CATCGAGAGG GAGGTCCTCA TTGTTGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA TCCTAATGGC TTGTCAGATC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGTGA GAGCAAGCAG AAGACCAAGA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAAACATT TAGATTCCAG CTGAAAGAAT CGGACAAAGA CAGGAGACTG 
TCTGTAGAGA TTTGGGATTG GGATCTGACC AGCAGAAATG ACTTCATGGG ATCTTTGTCG 
TTTGGGATTT CTGAACTGCA GAAAGCTGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTGCCCGTG CCACCGGAAG GCAGTGAGGG CAACGAGGAA 
CTGCGACAGA AATTCGAGAG AGCCAAGATC GGTCCTGGGC CCAAGACTCC TGAAGAAAAG 
ACGACCAACA CCATCTCCAA ATTTGACAAC AACGGCAACA GGGACCGGAT GAAATTGACC 
GATTTTAACT TCCTGATGGT GCTGGGAAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGAAAAGGCA CAGATGAGCT GTATGCTGTG AAGATCCTGA AAAAGGACGT GGTCATCCAA 
GATGATGACG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ATCGCCTGTA CTTCGTGATG 
GAGTATGTGA ATGGTGGTGA CCTCATGTAC CACATCCAGC AAGTGGGCCG GTTCAAGGAG 
CCTCACGCCG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC GACTAAGACC 
TTCTGCGGCA CCCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGCGTCCTG CTGTATGAGA TGTTGGCTGG GCAGGCACCG 
TTTGAAGGGG AGGACGAGGA TGAACTCTTC CAGTCCATCA TGGAACACAA CGTGGCTTAT 
CCGAAGTCCA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ACTGGGAGAA ACTTGAACGC AAAGAGATTC AGCCCCCGTA TAAGCCGAAA 
GCCAGAGACA AGCGAGACAC CTCCAATTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTCACGCCTA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCCTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( XM_005224690.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224747.1 Bos taurus protein kinase C beta (PRKCB), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230152
Clone ID Related Accession (Same CDS sequence)	XM_005224690.4
Accession Version	XM_005224690.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C beta type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224690.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CGGCCGCGGG GCCGCCGCCG AGCGAGGGAG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTTCAGTG CCAAGTCTGC TGTTTTGTCG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC GGGCGCTGAC AAGGGCCCAG CCTCTGACGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACATACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
TTGCTCTATG GACTTATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAAGCCCA CATCGAGAGG GAGGTCCTCA TTGTTGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA TCCTAATGGC TTGTCAGATC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGTGA GAGCAAGCAG AAGACCAAGA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAAACATT TAGATTCCAG CTGAAAGAAT CGGACAAAGA CAGGAGACTG 
TCTGTAGAGA TTTGGGATTG GGATCTGACC AGCAGAAATG ACTTCATGGG ATCTTTGTCG 
TTTGGGATTT CTGAACTGCA GAAAGCTGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTGCCCGTG CCACCGGAAG GCAGTGAGGG CAACGAGGAA 
CTGCGACAGA AATTCGAGAG AGCCAAGATC GGTCCTGGGC CCAAGACTCC TGAAGAAAAG 
ACGACCAACA CCATCTCCAA ATTTGACAAC AACGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGAAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGAAAAGGCA CAGATGAGCT GTATGCTGTG AAGATCCTGA AAAAGGACGT GGTCATCCAA 
GATGATGACG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ATCGCCTGTA CTTCGTGATG 
GAGTATGTGA ATGGTGGTGA CCTCATGTAC CACATCCAGC AAGTGGGCCG GTTCAAGGAG 
CCTCACGCCG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC GACTAAGACC 
TTCTGCGGCA CCCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGCGTCCTG CTGTATGAGA TGTTGGCTGG GCAGGCACCG 
TTTGAAGGGG AGGACGAGGA TGAACTCTTC CAGTCCATCA TGGAACACAA CGTGGCTTAT 
CCGAAGTCCA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ACTGGGAGAA ACTTGAACGC AAAGAGATTC AGCCCCCGTA TAAGCCGAAA 
GCCAGAGACA AGCGAGACAC CTCCAATTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTCACGCCTA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCCTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224747.1
CDS	364..2379
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIEREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGPGPKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV

Target ORF information:

RefSeq Version	XM_005224690.4
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C beta (PRKCB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224690.4

ORF Insert Sequence:

ATGGCTGACC CGGCCGCGGG GCCGCCGCCG AGCGAGGGAG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTTCAGTG CCAAGTCTGC TGTTTTGTCG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC GGGCGCTGAC AAGGGCCCAG CCTCTGACGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACATACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
TTGCTCTATG GACTTATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAAGCCCA CATCGAGAGG GAGGTCCTCA TTGTTGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA TCCTAATGGC TTGTCAGATC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGTGA GAGCAAGCAG AAGACCAAGA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAAACATT TAGATTCCAG CTGAAAGAAT CGGACAAAGA CAGGAGACTG 
TCTGTAGAGA TTTGGGATTG GGATCTGACC AGCAGAAATG ACTTCATGGG ATCTTTGTCG 
TTTGGGATTT CTGAACTGCA GAAAGCTGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTGCCCGTG CCACCGGAAG GCAGTGAGGG CAACGAGGAA 
CTGCGACAGA AATTCGAGAG AGCCAAGATC GGTCCTGGGC CCAAGACTCC TGAAGAAAAG 
ACGACCAACA CCATCTCCAA ATTTGACAAC AACGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGAAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGAAAAGGCA CAGATGAGCT GTATGCTGTG AAGATCCTGA AAAAGGACGT GGTCATCCAA 
GATGATGACG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ATCGCCTGTA CTTCGTGATG 
GAGTATGTGA ATGGTGGTGA CCTCATGTAC CACATCCAGC AAGTGGGCCG GTTCAAGGAG 
CCTCACGCCG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC GACTAAGACC 
TTCTGCGGCA CCCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGCGTCCTG CTGTATGAGA TGTTGGCTGG GCAGGCACCG 
TTTGAAGGGG AGGACGAGGA TGAACTCTTC CAGTCCATCA TGGAACACAA CGTGGCTTAT 
CCGAAGTCCA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ACTGGGAGAA ACTTGAACGC AAAGAGATTC AGCCCCCGTA TAAGCCGAAA 
GCCAGAGACA AGCGAGACAC CTCCAATTTC GACAAAGAGT TCACCAGACA GCCTGTGGAA 
CTCACGCCTA CTGATAAACT CTTCATCATG AACTTGGACC AAAATGAATT TGCTGGCTTC 
TCCTATACTA ACCCAGAGTT TGTCATTAAT GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( XM_024984661.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840429.1 Bos taurus protein kinase C beta (PRKCB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa230153
Clone ID Related Accession (Same CDS sequence)	XM_024984661.1
Accession Version	XM_024984661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C beta type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACC CGGCCGCGGG GCCGCCGCCG AGCGAGGGAG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTTCAGTG CCAAGTCTGC TGTTTTGTCG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC GGGCGCTGAC AAGGGCCCAG CCTCTGACGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACATACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
TTGCTCTATG GACTTATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAAGCCCA CATCGAGAGG GAGGTCCTCA TTGTTGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA TCCTAATGGC TTGTCAGATC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGTGA GAGCAAGCAG AAGACCAAGA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAAACATT TAGATTCCAG CTGAAAGAAT CGGACAAAGA CAGGAGACTG 
TCTGTAGAGA TTTGGGATTG GGATCTGACC AGCAGAAATG ACTTCATGGG ATCTTTGTCG 
TTTGGGATTT CTGAACTGCA GAAAGCTGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTGCCCGTG CCACCGGAAG GCAGTGAGGG CAACGAGGAA 
CTGCGACAGA AATTCGAGAG AGCCAAGATC GGTCCTGGGC CCAAGACTCC TGAAGAAAAG 
ACGACCAACA CCATCTCCAA ATTTGACAAC AACGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGAAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGAAAAGGCA CAGATGAGCT GTATGCTGTG AAGATCCTGA AAAAGGACGT GGTCATCCAA 
GATGATGACG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ATCGCCTGTA CTTCGTGATG 
GAGTATGTGA ATGGTGGTGA CCTCATGTAC CACATCCAGC AAGTGGGCCG GTTCAAGGAG 
CCTCACGCCG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC GACTAAGACC 
TTCTGCGGCA CCCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGCGTCCTG CTGTATGAGA TGTTGGCTGG GCAGGCACCG 
TTTGAAGGGG AGGACGAGGA TGAACTCTTC CAGTCCATCA TGGAACACAA CGTGGCTTAT 
CCGAAGTCCA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ACTGGGAGAA ACTTGAACGC AAAGAGATTC AGCCCCCGTA TAAGCCGAAA 
GCCGAGCCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840429.1
CDS	187..2061
Translation	MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIEREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGPGPKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKAEPQ

Target ORF information:

RefSeq Version	XM_024984661.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C beta (PRKCB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984661.1

ORF Insert Sequence:

ATGGCTGACC CGGCCGCGGG GCCGCCGCCG AGCGAGGGAG AGGAGAGCAC CGTGCGCTTC 
GCCCGCAAAG GCGCCCTCCG GCAGAAGAAC GTGCACGAGG TGAAGAACCA CAAATTCACC 
GCCCGCTTCT TCAAGCAGCC CACCTTCTGC AGCCACTGCA CCGACTTCAT CTGGGGCTTC 
GGGAAGCAAG GATTTCAGTG CCAAGTCTGC TGTTTTGTCG TGCACAAGCG GTGCCATGAA 
TTTGTCACCT TCTCCTGCCC GGGCGCTGAC AAGGGCCCAG CCTCTGACGA CCCCCGGAGC 
AAACACAAGT TCAAGATCCA CACATACTCC AGCCCCACGT TTTGTGACCA CTGTGGGTCA 
TTGCTCTATG GACTTATCCA CCAGGGGATG AAATGTGACA CCTGCATGAT GAACGTCCAC 
AAGCGCTGCG TGATGAACGT CCCCAGCCTC TGCGGCACGG ACCACACGGA GCGCCGCGGC 
CGCATCTACA TCCAAGCCCA CATCGAGAGG GAGGTCCTCA TTGTTGTCGT AAGAGATGCT 
AAAAACCTTG TACCTATGGA TCCTAATGGC TTGTCAGATC CTTACGTGAA ACTGAAACTG 
ATTCCTGATC CCAAGAGTGA GAGCAAGCAG AAGACCAAGA CCATCAAGTG CTCCCTCAAC 
CCCGAGTGGA ACGAAACATT TAGATTCCAG CTGAAAGAAT CGGACAAAGA CAGGAGACTG 
TCTGTAGAGA TTTGGGATTG GGATCTGACC AGCAGAAATG ACTTCATGGG ATCTTTGTCG 
TTTGGGATTT CTGAACTGCA GAAAGCTGGT GTTGATGGCT GGTTTAAGTT ACTGAGCCAG 
GAGGAAGGCG AGTACTTCAA CGTGCCCGTG CCACCGGAAG GCAGTGAGGG CAACGAGGAA 
CTGCGACAGA AATTCGAGAG AGCCAAGATC GGTCCTGGGC CCAAGACTCC TGAAGAAAAG 
ACGACCAACA CCATCTCCAA ATTTGACAAC AACGGCAACA GGGACCGGAT GAAACTGACC 
GATTTTAACT TCCTGATGGT GCTGGGAAAA GGCAGCTTTG GCAAGGTCAT GCTCTCAGAG 
CGAAAAGGCA CAGATGAGCT GTATGCTGTG AAGATCCTGA AAAAGGACGT GGTCATCCAA 
GATGATGACG TGGAATGCAC GATGGTGGAG AAGCGGGTGC TGGCCCTGCC CGGGAAGCCG 
CCCTTCCTGA CCCAGCTGCA TTCCTGCTTC CAGACCATGG ATCGCCTGTA CTTCGTGATG 
GAGTATGTGA ATGGTGGTGA CCTCATGTAC CACATCCAGC AAGTGGGCCG GTTCAAGGAG 
CCTCACGCCG TATTTTACGC TGCAGAAATT GCCATCGGTC TGTTCTTCTT GCAGAGCAAG 
GGCATCATTT ACCGTGACCT GAAACTTGAC AATGTGATGC TGGATTCCGA GGGCCACATC 
AAGATCGCTG ATTTTGGCAT GTGTAAGGAG AACATCTGGG ATGGGGTGAC GACTAAGACC 
TTCTGCGGCA CCCCAGACTA CATCGCCCCC GAGATAATTG CTTATCAGCC CTATGGGAAG 
TCCGTGGATT GGTGGGCATT TGGCGTCCTG CTGTATGAGA TGTTGGCTGG GCAGGCACCG 
TTTGAAGGGG AGGACGAGGA TGAACTCTTC CAGTCCATCA TGGAACACAA CGTGGCTTAT 
CCGAAGTCCA TGTCCAAGGA AGCTGTGGCC ATCTGCAAAG GGCTGATGAC CAAACACCCA 
GGCAAACGTC TGGGTTGTGG ACCTGAAGGT GAACGTGACA TCAAAGAGCA TGCATTTTTC 
CGGTATATTG ACTGGGAGAA ACTTGAACGC AAAGAGATTC AGCCCCCGTA TAAGCCGAAA 
GCCGAGCCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( XM_015460312.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315798.1 Bos taurus protein kinase C beta (PRKCB), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa230154
Clone ID Related Accession (Same CDS sequence)	XM_015460312.2
Accession Version	XM_015460312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C beta type isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTTG TCAGCAAAGA CAGTGAAGAC CGCCTAGAGA GAGTGGGGAT CATTTTGTTC 
TGCTGTTTTG TCGTGCACAA GCGGTGCCAT GAATTTGTCA CCTTCTCCTG CCCGGGCGCT 
GACAAGGGCC CAGCCTCTGA CGACCCCCGG AGCAAACACA AGTTCAAGAT CCACACATAC 
TCCAGCCCCA CGTTTTGTGA CCACTGTGGG TCATTGCTCT ATGGACTTAT CCACCAGGGG 
ATGAAATGTG ACACCTGCAT GATGAACGTC CACAAGCGCT GCGTGATGAA CGTCCCCAGC 
CTCTGCGGCA CGGACCACAC GGAGCGCCGC GGCCGCATCT ACATCCAAGC CCACATCGAG 
AGGGAGGTCC TCATTGTTGT CGTAAGAGAT GCTAAAAACC TTGTACCTAT GGATCCTAAT 
GGCTTGTCAG ATCCTTACGT GAAACTGAAA CTGATTCCTG ATCCCAAGAG TGAGAGCAAG 
CAGAAGACCA AGACCATCAA GTGCTCCCTC AACCCCGAGT GGAACGAAAC ATTTAGATTC 
CAGCTGAAAG AATCGGACAA AGACAGGAGA CTGTCTGTAG AGATTTGGGA TTGGGATCTG 
ACCAGCAGAA ATGACTTCAT GGGATCTTTG TCGTTTGGGA TTTCTGAACT GCAGAAAGCT 
GGTGTTGATG GCTGGTTTAA GTTACTGAGC CAGGAGGAAG GCGAGTACTT CAACGTGCCC 
GTGCCACCGG AAGGCAGTGA GGGCAACGAG GAACTGCGAC AGAAATTCGA GAGAGCCAAG 
ATCGGTCCTG GGCCCAAGAC TCCTGAAGAA AAGACGACCA ACACCATCTC CAAATTTGAC 
AACAACGGCA ACAGGGACCG GATGAAACTG ACCGATTTTA ACTTCCTGAT GGTGCTGGGA 
AAAGGCAGCT TTGGCAAGGT CATGCTCTCA GAGCGAAAAG GCACAGATGA GCTGTATGCT 
GTGAAGATCC TGAAAAAGGA CGTGGTCATC CAAGATGATG ACGTGGAATG CACGATGGTG 
GAGAAGCGGG TGCTGGCCCT GCCCGGGAAG CCGCCCTTCC TGACCCAGCT GCATTCCTGC 
TTCCAGACCA TGGATCGCCT GTACTTCGTG ATGGAGTATG TGAATGGTGG TGACCTCATG 
TACCACATCC AGCAAGTGGG CCGGTTCAAG GAGCCTCACG CCGTATTTTA CGCTGCAGAA 
ATTGCCATCG GTCTGTTCTT CTTGCAGAGC AAGGGCATCA TTTACCGTGA CCTGAAACTT 
GACAATGTGA TGCTGGATTC CGAGGGCCAC ATCAAGATCG CTGATTTTGG CATGTGTAAG 
GAGAACATCT GGGATGGGGT GACGACTAAG ACCTTCTGCG GCACCCCAGA CTACATCGCC 
CCCGAGATAA TTGCTTATCA GCCCTATGGG AAGTCCGTGG ATTGGTGGGC ATTTGGCGTC 
CTGCTGTATG AGATGTTGGC TGGGCAGGCA CCGTTTGAAG GGGAGGACGA GGATGAACTC 
TTCCAGTCCA TCATGGAACA CAACGTGGCT TATCCGAAGT CCATGTCCAA GGAAGCTGTG 
GCCATCTGCA AAGGGCTGAT GACCAAACAC CCAGGCAAAC GTCTGGGTTG TGGACCTGAA 
GGTGAACGTG ACATCAAAGA GCATGCATTT TTCCGGTATA TTGACTGGGA GAAACTTGAA 
CGCAAAGAGA TTCAGCCCCC GTATAAGCCG AAAGCCTGTG GGCGAAATGC TGAAAACTTC 
GACCGATTTT TCACCCGCCA TCCACCAGTT CTAACACCTC CTGACCAGGA AGTCATCAGG 
AATATTGACC AATCAGAATT CGAAGGATTT TCCTTTGTTA ACTCTGAATT TTTAAAACCT 
GAAGTCAAGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315798.1
CDS	16..1890
Translation	MGFVSKDSEDRLERVGIILFCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIEREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGPGPKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS

Target ORF information:

RefSeq Version	XM_015460312.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C beta (PRKCB), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460312.2

ORF Insert Sequence:

ATGGGGTTTG TCAGCAAAGA CAGTGAAGAC CGCCTAGAGA GAGTGGGGAT CATTTTGTTC 
TGCTGTTTTG TCGTGCACAA GCGGTGCCAT GAATTTGTCA CCTTCTCCTG CCCGGGCGCT 
GACAAGGGCC CAGCCTCTGA CGACCCCCGG AGCAAACACA AGTTCAAGAT CCACACATAC 
TCCAGCCCCA CGTTTTGTGA CCACTGTGGG TCATTGCTCT ATGGACTTAT CCACCAGGGG 
ATGAAATGTG ACACCTGCAT GATGAACGTC CACAAGCGCT GCGTGATGAA CGTCCCCAGC 
CTCTGCGGCA CGGACCACAC GGAGCGCCGC GGCCGCATCT ACATCCAAGC CCACATCGAG 
AGGGAGGTCC TCATTGTTGT CGTAAGAGAT GCTAAAAACC TTGTACCTAT GGATCCTAAT 
GGCTTGTCAG ATCCTTACGT GAAACTGAAA CTGATTCCTG ATCCCAAGAG TGAGAGCAAG 
CAGAAGACCA AGACCATCAA GTGCTCCCTC AACCCCGAGT GGAACGAAAC ATTTAGATTC 
CAGCTGAAAG AATCGGACAA AGACAGGAGA CTGTCTGTAG AGATTTGGGA TTGGGATCTG 
ACCAGCAGAA ATGACTTCAT GGGATCTTTG TCGTTTGGGA TTTCTGAACT GCAGAAAGCT 
GGTGTTGATG GCTGGTTTAA GTTACTGAGC CAGGAGGAAG GCGAGTACTT CAACGTGCCC 
GTGCCACCGG AAGGCAGTGA GGGCAACGAG GAACTGCGAC AGAAATTCGA GAGAGCCAAG 
ATCGGTCCTG GGCCCAAGAC TCCTGAAGAA AAGACGACCA ACACCATCTC CAAATTTGAC 
AACAACGGCA ACAGGGACCG GATGAAACTG ACCGATTTTA ACTTCCTGAT GGTGCTGGGA 
AAAGGCAGCT TTGGCAAGGT CATGCTCTCA GAGCGAAAAG GCACAGATGA GCTGTATGCT 
GTGAAGATCC TGAAAAAGGA CGTGGTCATC CAAGATGATG ACGTGGAATG CACGATGGTG 
GAGAAGCGGG TGCTGGCCCT GCCCGGGAAG CCGCCCTTCC TGACCCAGCT GCATTCCTGC 
TTCCAGACCA TGGATCGCCT GTACTTCGTG ATGGAGTATG TGAATGGTGG TGACCTCATG 
TACCACATCC AGCAAGTGGG CCGGTTCAAG GAGCCTCACG CCGTATTTTA CGCTGCAGAA 
ATTGCCATCG GTCTGTTCTT CTTGCAGAGC AAGGGCATCA TTTACCGTGA CCTGAAACTT 
GACAATGTGA TGCTGGATTC CGAGGGCCAC ATCAAGATCG CTGATTTTGG CATGTGTAAG 
GAGAACATCT GGGATGGGGT GACGACTAAG ACCTTCTGCG GCACCCCAGA CTACATCGCC 
CCCGAGATAA TTGCTTATCA GCCCTATGGG AAGTCCGTGG ATTGGTGGGC ATTTGGCGTC 
CTGCTGTATG AGATGTTGGC TGGGCAGGCA CCGTTTGAAG GGGAGGACGA GGATGAACTC 
TTCCAGTCCA TCATGGAACA CAACGTGGCT TATCCGAAGT CCATGTCCAA GGAAGCTGTG 
GCCATCTGCA AAGGGCTGAT GACCAAACAC CCAGGCAAAC GTCTGGGTTG TGGACCTGAA 
GGTGAACGTG ACATCAAAGA GCATGCATTT TTCCGGTATA TTGACTGGGA GAAACTTGAA 
CGCAAAGAGA TTCAGCCCCC GTATAAGCCG AAAGCCTGTG GGCGAAATGC TGAAAACTTC 
GACCGATTTT TCACCCGCCA TCCACCAGTT CTAACACCTC CTGACCAGGA AGTCATCAGG 
AATATTGACC AATCAGAATT CGAAGGATTT TCCTTTGTTA ACTCTGAATT TTTAAAACCT 
GAAGTCAAGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( XM_024984662.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840430.1 Bos taurus protein kinase C beta (PRKCB), transcript variant X4, mRNA.

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CloneID	OBa230155
Clone ID Related Accession (Same CDS sequence)	XM_024984662.1
Accession Version	XM_024984662.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein kinase C beta type isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGA GAGCACTTAG GACATTCTTC CAGAATGTTC TCTGTACCAG CCAGCAGATA 
GCTGTGACCC TCCTCAGGTG GCTCTCCCAC AGTCCCAGTG GCTCTCTTGA GTCCCTCCCT 
GGACCTCCCC CCAGCCCAGC AACTAAATTC ACACCTGAGC ATCTAGAGGG ACCTCCAAGA 
CCCTGTTCGA TTTCTGAGGA TGGCATCAGT ACTGTAAGAG ATGCTAAAAA CCTTGTACCT 
ATGGATCCTA ATGGCTTGTC AGATCCTTAC GTGAAACTGA AACTGATTCC TGATCCCAAG 
AGTGAGAGCA AGCAGAAGAC CAAGACCATC AAGTGCTCCC TCAACCCCGA GTGGAACGAA 
ACATTTAGAT TCCAGCTGAA AGAATCGGAC AAAGACAGGA GACTGTCTGT AGAGATTTGG 
GATTGGGATC TGACCAGCAG AAATGACTTC ATGGGATCTT TGTCGTTTGG GATTTCTGAA 
CTGCAGAAAG CTGGTGTTGA TGGCTGGTTT AAGTTACTGA GCCAGGAGGA AGGCGAGTAC 
TTCAACGTGC CCGTGCCACC GGAAGGCAGT GAGGGCAACG AGGAACTGCG ACAGAAATTC 
GAGAGAGCCA AGATCGGTCC TGGGCCCAAG ACTCCTGAAG AAAAGACGAC CAACACCATC 
TCCAAATTTG ACAACAACGG CAACAGGGAC CGGATGAAAC TGACCGATTT TAACTTCCTG 
ATGGTGCTGG GAAAAGGCAG CTTTGGCAAG GTCATGCTCT CAGAGCGAAA AGGCACAGAT 
GAGCTGTATG CTGTGAAGAT CCTGAAAAAG GACGTGGTCA TCCAAGATGA TGACGTGGAA 
TGCACGATGG TGGAGAAGCG GGTGCTGGCC CTGCCCGGGA AGCCGCCCTT CCTGACCCAG 
CTGCATTCCT GCTTCCAGAC CATGGATCGC CTGTACTTCG TGATGGAGTA TGTGAATGGT 
GGTGACCTCA TGTACCACAT CCAGCAAGTG GGCCGGTTCA AGGAGCCTCA CGCCGTATTT 
TACGCTGCAG AAATTGCCAT CGGTCTGTTC TTCTTGCAGA GCAAGGGCAT CATTTACCGT 
GACCTGAAAC TTGACAATGT GATGCTGGAT TCCGAGGGCC ACATCAAGAT CGCTGATTTT 
GGCATGTGTA AGGAGAACAT CTGGGATGGG GTGACGACTA AGACCTTCTG CGGCACCCCA 
GACTACATCG CCCCCGAGAT AATTGCTTAT CAGCCCTATG GGAAGTCCGT GGATTGGTGG 
GCATTTGGCG TCCTGCTGTA TGAGATGTTG GCTGGGCAGG CACCGTTTGA AGGGGAGGAC 
GAGGATGAAC TCTTCCAGTC CATCATGGAA CACAACGTGG CTTATCCGAA GTCCATGTCC 
AAGGAAGCTG TGGCCATCTG CAAAGGGCTG ATGACCAAAC ACCCAGGCAA ACGTCTGGGT 
TGTGGACCTG AAGGTGAACG TGACATCAAA GAGCATGCAT TTTTCCGGTA TATTGACTGG 
GAGAAACTTG AACGCAAAGA GATTCAGCCC CCGTATAAGC CGAAAGCCTG TGGGCGAAAT 
GCTGAAAACT TCGACCGATT TTTCACCCGC CATCCACCAG TTCTAACACC TCCTGACCAG 
GAAGTCATCA GGAATATTGA CCAATCAGAA TTCGAAGGAT TTTCCTTTGT TAACTCTGAA 
TTTTTAAAAC CTGAAGTCAA GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840430.1
CDS	25..1731
Translation	MCMRALRTFFQNVLCTSQQIAVTLLRWLSHSPSGSLESLPGPPPSPATKFTPEHLEGPPRPCSISEDGISTVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGPGPKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS

Target ORF information:

RefSeq Version	XM_024984662.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C beta (PRKCB), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984662.1

ORF Insert Sequence:

ATGTGCATGA GAGCACTTAG GACATTCTTC CAGAATGTTC TCTGTACCAG CCAGCAGATA 
GCTGTGACCC TCCTCAGGTG GCTCTCCCAC AGTCCCAGTG GCTCTCTTGA GTCCCTCCCT 
GGACCTCCCC CCAGCCCAGC AACTAAATTC ACACCTGAGC ATCTAGAGGG ACCTCCAAGA 
CCCTGTTCGA TTTCTGAGGA TGGCATCAGT ACTGTAAGAG ATGCTAAAAA CCTTGTACCT 
ATGGATCCTA ATGGCTTGTC AGATCCTTAC GTGAAACTGA AACTGATTCC TGATCCCAAG 
AGTGAGAGCA AGCAGAAGAC CAAGACCATC AAGTGCTCCC TCAACCCCGA GTGGAACGAA 
ACATTTAGAT TCCAGCTGAA AGAATCGGAC AAAGACAGGA GACTGTCTGT AGAGATTTGG 
GATTGGGATC TGACCAGCAG AAATGACTTC ATGGGATCTT TGTCGTTTGG GATTTCTGAA 
CTGCAGAAAG CTGGTGTTGA TGGCTGGTTT AAGTTACTGA GCCAGGAGGA AGGCGAGTAC 
TTCAACGTGC CCGTGCCACC GGAAGGCAGT GAGGGCAACG AGGAACTGCG ACAGAAATTC 
GAGAGAGCCA AGATCGGTCC TGGGCCCAAG ACTCCTGAAG AAAAGACGAC CAACACCATC 
TCCAAATTTG ACAACAACGG CAACAGGGAC CGGATGAAAC TGACCGATTT TAACTTCCTG 
ATGGTGCTGG GAAAAGGCAG CTTTGGCAAG GTCATGCTCT CAGAGCGAAA AGGCACAGAT 
GAGCTGTATG CTGTGAAGAT CCTGAAAAAG GACGTGGTCA TCCAAGATGA TGACGTGGAA 
TGCACGATGG TGGAGAAGCG GGTGCTGGCC CTGCCCGGGA AGCCGCCCTT CCTGACCCAG 
CTGCATTCCT GCTTCCAGAC CATGGATCGC CTGTACTTCG TGATGGAGTA TGTGAATGGT 
GGTGACCTCA TGTACCACAT CCAGCAAGTG GGCCGGTTCA AGGAGCCTCA CGCCGTATTT 
TACGCTGCAG AAATTGCCAT CGGTCTGTTC TTCTTGCAGA GCAAGGGCAT CATTTACCGT 
GACCTGAAAC TTGACAATGT GATGCTGGAT TCCGAGGGCC ACATCAAGAT CGCTGATTTT 
GGCATGTGTA AGGAGAACAT CTGGGATGGG GTGACGACTA AGACCTTCTG CGGCACCCCA 
GACTACATCG CCCCCGAGAT AATTGCTTAT CAGCCCTATG GGAAGTCCGT GGATTGGTGG 
GCATTTGGCG TCCTGCTGTA TGAGATGTTG GCTGGGCAGG CACCGTTTGA AGGGGAGGAC 
GAGGATGAAC TCTTCCAGTC CATCATGGAA CACAACGTGG CTTATCCGAA GTCCATGTCC 
AAGGAAGCTG TGGCCATCTG CAAAGGGCTG ATGACCAAAC ACCCAGGCAA ACGTCTGGGT 
TGTGGACCTG AAGGTGAACG TGACATCAAA GAGCATGCAT TTTTCCGGTA TATTGACTGG 
GAGAAACTTG AACGCAAAGA GATTCAGCCC CCGTATAAGC CGAAAGCCTG TGGGCGAAAT 
GCTGAAAACT TCGACCGATT TTTCACCCGC CATCCACCAG TTCTAACACC TCCTGACCAG 
GAAGTCATCA GGAATATTGA CCAATCAGAA TTCGAAGGAT TTTCCTTTGT TAACTCTGAA 
TTTTTAAAAC CTGAAGTCAA GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKCB ( XM_015460312.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315798.1 Bos taurus protein kinase C, beta (PRKCB), transcript variant X2, mRNA.

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CloneID	OBa88480
Clone ID Related Accession (Same CDS sequence)	XM_015460312.1
Accession Version	XM_015460312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein kinase C beta type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTTTG TCAGCAAAGA CAGTGAAGAC CGCCTAGAGA GAGTGGGGAT CATTTTGTTC 
TGCTGTTTTG TCGTGCACAA GCGGTGCCAT GAATTTGTCA CCTTCTCCTG CCCGGGCGCT 
GACAAGGGCC CAGCCTCTGA CGACCCCCGG AGCAAACACA AGTTCAAGAT CCACACATAC 
TCCAGCCCCA CGTTTTGTGA CCACTGTGGG TCATTGCTCT ATGGACTTAT CCACCAGGGG 
ATGAAATGTG ACACCTGCAT GATGAACGTC CACAAGCGCT GCGTGATGAA CGTCCCCAGC 
CTCTGCGGCA CGGACCACAC GGAGCGCCGC GGCCGCATCT ACATCCAAGC CCACATCGAG 
AGGGAGGTCC TCATTGTTGT CGTAAGAGAT GCTAAAAACC TTGTACCTAT GGATCCTAAT 
GGCTTGTCAG ATCCTTACGT GAAACTGAAA CTGATTCCTG ATCCCAAGAG TGAGAGCAAG 
CAGAAGACCA AGACCATCAA GTGCTCCCTC AACCCCGAGT GGAACGAAAC ATTTAGATTC 
CAGCTGAAAG AATCGGACAA AGACAGGAGA CTGTCTGTAG AGATTTGGGA TTGGGATCTG 
ACCAGCAGAA ATGACTTCAT GGGATCTTTG TCGTTTGGGA TTTCTGAACT GCAGAAAGCT 
GGTGTTGATG GCTGGTTTAA GTTACTGAGC CAGGAGGAAG GCGAGTACTT CAACGTGCCC 
GTGCCACCGG AAGGCAGTGA GGGCAACGAG GAACTGCGAC AGAAATTCGA GAGAGCCAAG 
ATCGGTCCTG GGCCCAAGAC TCCTGAAGAA AAGACGACCA ACACCATCTC CAAATTTGAC 
AACAACGGCA ACAGGGACCG GATGAAATTG ACCGATTTTA ACTTCCTGAT GGTGCTGGGA 
AAAGGCAGCT TTGGCAAGGT CATGCTCTCA GAGCGAAAAG GCACAGATGA GCTGTATGCT 
GTGAAGATCC TGAAAAAGGA CGTGGTCATC CAAGATGATG ACGTGGAATG CACGATGGTG 
GAGAAGCGGG TGCTGGCCCT GCCCGGGAAG CCGCCCTTCC TGACCCAGCT GCATTCCTGC 
TTCCAGACCA TGGATCGCCT GTACTTCGTG ATGGAGTATG TGAATGGTGG TGACCTCATG 
TACCACATCC AGCAAGTGGG CCGGTTCAAG GAGCCTCACG CCGTATTTTA CGCTGCAGAA 
ATTGCCATCG GTCTGTTCTT CTTGCAGAGC AAGGGCATCA TTTACCGTGA CCTGAAACTT 
GACAATGTGA TGCTGGATTC CGAGGGCCAC ATCAAGATCG CTGATTTTGG CATGTGTAAG 
GAGAACATCT GGGATGGGGT GACGACTAAG ACCTTCTGCG GCACCCCAGA CTACATCGCC 
CCCGAGATAA TTGCTTATCA GCCCTATGGG AAGTCCGTGG ATTGGTGGGC ATTTGGCGTC 
CTGCTGTATG AGATGTTGGC TGGGCAGGCA CCGTTTGAAG GGGAGGACGA GGATGAACTC 
TTCCAGTCCA TCATGGAACA CAACGTGGCT TATCCGAAGT CCATGTCCAA GGAAGCTGTG 
GCCATCTGCA AAGGGCTGAT GACCAAACAC CCAGGCAAAC GTCTGGGTTG TGGACCTGAA 
GGTGAACGTG ACATCAAAGA GCATGCATTT TTCCGGTATA TTGACTGGGA GAAACTTGAA 
CGCAAAGAGA TTCAGCCCCC GTATAAGCCG AAAGCCTGTG GGCGAAATGC TGAAAACTTC 
GACCGATTTT TCACCCGCCA TCCACCAGTT CTAACACCTC CTGACCAGGA AGTCATCAGG 
AATATTGACC AATCAGAATT CGAAGGATTT TCCTTTGTTA ACTCTGAATT TTTAAAACCT 
GAAGTCAAGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315798.1
CDS	148..2022
Translation	MGFVSKDSEDRLERVGIILFCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIEREVLIVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKAGVDGWFKLLSQEEGEYFNVPVPPEGSEGNEELRQKFERAKIGPGPKTPEEKTTNTISKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKACGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFVNSEFLKPEVKS

Target ORF information:

RefSeq Version	XM_015460312.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase C, beta (PRKCB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460312.1

ORF Insert Sequence:

ATGGGGTTTG TCAGCAAAGA CAGTGAAGAC CGCCTAGAGA GAGTGGGGAT CATTTTGTTC 
TGCTGTTTTG TCGTGCACAA GCGGTGCCAT GAATTTGTCA CCTTCTCCTG CCCGGGCGCT 
GACAAGGGCC CAGCCTCTGA CGACCCCCGG AGCAAACACA AGTTCAAGAT CCACACATAC 
TCCAGCCCCA CGTTTTGTGA CCACTGTGGG TCATTGCTCT ATGGACTTAT CCACCAGGGG 
ATGAAATGTG ACACCTGCAT GATGAACGTC CACAAGCGCT GCGTGATGAA CGTCCCCAGC 
CTCTGCGGCA CGGACCACAC GGAGCGCCGC GGCCGCATCT ACATCCAAGC CCACATCGAG 
AGGGAGGTCC TCATTGTTGT CGTAAGAGAT GCTAAAAACC TTGTACCTAT GGATCCTAAT 
GGCTTGTCAG ATCCTTACGT GAAACTGAAA CTGATTCCTG ATCCCAAGAG TGAGAGCAAG 
CAGAAGACCA AGACCATCAA GTGCTCCCTC AACCCCGAGT GGAACGAAAC ATTTAGATTC 
CAGCTGAAAG AATCGGACAA AGACAGGAGA CTGTCTGTAG AGATTTGGGA TTGGGATCTG 
ACCAGCAGAA ATGACTTCAT GGGATCTTTG TCGTTTGGGA TTTCTGAACT GCAGAAAGCT 
GGTGTTGATG GCTGGTTTAA GTTACTGAGC CAGGAGGAAG GCGAGTACTT CAACGTGCCC 
GTGCCACCGG AAGGCAGTGA GGGCAACGAG GAACTGCGAC AGAAATTCGA GAGAGCCAAG 
ATCGGTCCTG GGCCCAAGAC TCCTGAAGAA AAGACGACCA ACACCATCTC CAAATTTGAC 
AACAACGGCA ACAGGGACCG GATGAAATTG ACCGATTTTA ACTTCCTGAT GGTGCTGGGA 
AAAGGCAGCT TTGGCAAGGT CATGCTCTCA GAGCGAAAAG GCACAGATGA GCTGTATGCT 
GTGAAGATCC TGAAAAAGGA CGTGGTCATC CAAGATGATG ACGTGGAATG CACGATGGTG 
GAGAAGCGGG TGCTGGCCCT GCCCGGGAAG CCGCCCTTCC TGACCCAGCT GCATTCCTGC 
TTCCAGACCA TGGATCGCCT GTACTTCGTG ATGGAGTATG TGAATGGTGG TGACCTCATG 
TACCACATCC AGCAAGTGGG CCGGTTCAAG GAGCCTCACG CCGTATTTTA CGCTGCAGAA 
ATTGCCATCG GTCTGTTCTT CTTGCAGAGC AAGGGCATCA TTTACCGTGA CCTGAAACTT 
GACAATGTGA TGCTGGATTC CGAGGGCCAC ATCAAGATCG CTGATTTTGG CATGTGTAAG 
GAGAACATCT GGGATGGGGT GACGACTAAG ACCTTCTGCG GCACCCCAGA CTACATCGCC 
CCCGAGATAA TTGCTTATCA GCCCTATGGG AAGTCCGTGG ATTGGTGGGC ATTTGGCGTC 
CTGCTGTATG AGATGTTGGC TGGGCAGGCA CCGTTTGAAG GGGAGGACGA GGATGAACTC 
TTCCAGTCCA TCATGGAACA CAACGTGGCT TATCCGAAGT CCATGTCCAA GGAAGCTGTG 
GCCATCTGCA AAGGGCTGAT GACCAAACAC CCAGGCAAAC GTCTGGGTTG TGGACCTGAA 
GGTGAACGTG ACATCAAAGA GCATGCATTT TTCCGGTATA TTGACTGGGA GAAACTTGAA 
CGCAAAGAGA TTCAGCCCCC GTATAAGCCG AAAGCCTGTG GGCGAAATGC TGAAAACTTC 
GACCGATTTT TCACCCGCCA TCCACCAGTT CTAACACCTC CTGACCAGGA AGTCATCAGG 
AATATTGACC AATCAGAATT CGAAGGATTT TCCTTTGTTA ACTCTGAATT TTTAAAACCT 
GAAGTCAAGA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Serine phosphorylation sites on IRS2 activated by angiotensin II and protein kinase C to induce selective insulin resistance in endothelial cells.
Molecular and cellular biology33(16)3227-41(2013 Aug)
Park K,Li Q,Rask-Madsen C,Mima A,Mizutani K,Winnay J,Maeda Y,D'Aquino K,White MF,Feener EP,King GL

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
Nature334(6184)661-5(1988 Aug)
Nishizuka Y

Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways.
Science (New York, N.Y.)233(4766)859-66(1986 Aug)
Coussens L,Parker PJ,Rhee L,Yang-Feng TL,Chen E,Waterfield MD,Francke U,Ullrich A

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PRKCB cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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