PFKFB1 cDNA ORF clone, Bos taurus(cattle)

The following PFKFB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKFB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa43576 NM_174572.4
Latest version!
Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa35628 XM_010821985.3
Latest version!
Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa35630 XM_015461707.2
Latest version!
Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa35632 XM_005228210.4
Latest version!
Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa45422 XM_010821986.3
Latest version!
Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa238263 XM_024987771.1
Latest version!
Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBa35632 XM_005228210.3 Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa35630 XM_015461707.1 Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa35628 XM_010821985.2 Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa45422 XM_010821986.2 Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa43576
    Clone ID Related Accession (Same CDS sequence) NM_174572.4
    Accession Version NM_174572.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S55569.1. On Apr 28, 2006 this sequence version replaced NM_174572.3. ##Evidence-Data-START## Transcript exon combination :: S55569.1, M64323.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145477 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTCAAG AGATGGGAGA GCTCACCCAA ACCAGATTGC AGAAGATCTG GATTCCACAC 
    AACAACGGCA ACAGCAGGCT TCAACGGAGG AGGGGCTCAT CCATACCACA GTTTACAAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GAAAGACCTA CATCTCAACA
    AAGCTCACAC GCTATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAA
    TATCGACGAG AGGCAGTGAG CTACAAGAAC TACGAATTCT TTCTTCCCGA CAACATGGAG
    GCCCTACTTA TCAGGAAGCA GTGTGCCCTA GCAGCCCTGA AAGATGTTCA TAGCTATCTC
    AGCCATGAGG AAGGTCGTGT TGCAGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG
    TCACTGATTC TGCAGTTTGC TAAAGAACAT GGTTACAAGG TCTTTTTCAT TGAGTCCATT
    TGTAATGACC CTGACGTCAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGTCCTGAT
    TATATAGACT GTGATCGTGA AAAGGTTCTG GAAGATTTTC TAAAGAGAAT AGAGTGCTAT
    GAGGTCAACT ACCAACCCTT GGATGATGAA CTGGATAGCC ACCTGTCCTA CATCAAGATC
    TTCGACGTGG GCACACGCTA CATGGTGAAT CGTGTGCAGG ACCATATCCA GAGCCGCACA
    GTCTACTATC TCATGAACAT TCACGTCACA CCCCGCTCCA TCTACCTATG CCGGCACGGT
    GAGAGTGAAC TCAACCTCAG AGGCCGCATT GGAGGTGACT CTGGCCTCTC AGCTCGGGGC
    AAGCAGTATG CCTATGCCCT GGCCAATTTC ATTCAGTCCC AGGGTATCAG CTCACTGAAG
    GTTGGGACCA GCCACATGAA GAGGACTATC CAGACAGCTG AAGCCTTAGG ACTTCCCTAT
    GAGCAATGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA
    GAAATCCAAG AACACTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATACCGCTAC
    CGCTATCCTA AGGGAGAGTC TTACGAGGAT CTGGTCCAGC GTCTGGAGCC AGTTATAATG
    GAGCTAGAAC GGCAGGAAAA TGTATTGGTG ATCTGCCATC AGGCTGTCAT GCGGTGCCTC
    CTGGCCTACT TTCTGGACAA GAGCTCAGAT GAGTTGCCTT ATCTCAAGTG CCCTCTCCAC
    ACAGTGCTCA AACTTACACC TGTGGCTTAT GGCTGCAAAG TGGAGTCCAT CTACCTGAAT
    GTGGAGGCTG TAAACACACA TCGGGAGAAA CCTGAGAATG TGGACATCAC CCGGGAACCT
    GAAGAAGCCC TGGACACTGT TCCTGCCCAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776997.3
    CDS177..1592
    Translation

    Target ORF information:

    RefSeq Version NM_174572.4
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174572.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTCAAG AGATGGGAGA GCTCACCCAA ACCAGATTGC AGAAGATCTG GATTCCACAC 
    AACAACGGCA ACAGCAGGCT TCAACGGAGG AGGGGCTCAT CCATACCACA GTTTACAAAT
    TCCCCCACAA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GAAAGACCTA CATCTCAACA
    AAGCTCACAC GCTATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGCCAA
    TATCGACGAG AGGCAGTGAG CTACAAGAAC TACGAATTCT TTCTTCCCGA CAACATGGAG
    GCCCTACTTA TCAGGAAGCA GTGTGCCCTA GCAGCCCTGA AAGATGTTCA TAGCTATCTC
    AGCCATGAGG AAGGTCGTGT TGCAGTTTTT GATGCCACCA ACACTACCAG AGAACGACGG
    TCACTGATTC TGCAGTTTGC TAAAGAACAT GGTTACAAGG TCTTTTTCAT TGAGTCCATT
    TGTAATGACC CTGACGTCAT TGCAGAAAAC ATCAGGCAAG TGAAACTTGG CAGTCCTGAT
    TATATAGACT GTGATCGTGA AAAGGTTCTG GAAGATTTTC TAAAGAGAAT AGAGTGCTAT
    GAGGTCAACT ACCAACCCTT GGATGATGAA CTGGATAGCC ACCTGTCCTA CATCAAGATC
    TTCGACGTGG GCACACGCTA CATGGTGAAT CGTGTGCAGG ACCATATCCA GAGCCGCACA
    GTCTACTATC TCATGAACAT TCACGTCACA CCCCGCTCCA TCTACCTATG CCGGCACGGT
    GAGAGTGAAC TCAACCTCAG AGGCCGCATT GGAGGTGACT CTGGCCTCTC AGCTCGGGGC
    AAGCAGTATG CCTATGCCCT GGCCAATTTC ATTCAGTCCC AGGGTATCAG CTCACTGAAG
    GTTGGGACCA GCCACATGAA GAGGACTATC CAGACAGCTG AAGCCTTAGG ACTTCCCTAT
    GAGCAATGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAGGAGAT GACCTATGAA
    GAAATCCAAG AACACTACCC TGAAGAATTT GCACTGCGAG ACCAAGATAA ATACCGCTAC
    CGCTATCCTA AGGGAGAGTC TTACGAGGAT CTGGTCCAGC GTCTGGAGCC AGTTATAATG
    GAGCTAGAAC GGCAGGAAAA TGTATTGGTG ATCTGCCATC AGGCTGTCAT GCGGTGCCTC
    CTGGCCTACT TTCTGGACAA GAGCTCAGAT GAGTTGCCTT ATCTCAAGTG CCCTCTCCAC
    ACAGTGCTCA AACTTACACC TGTGGCTTAT GGCTGCAAAG TGGAGTCCAT CTACCTGAAT
    GTGGAGGCTG TAAACACACA TCGGGAGAAA CCTGAGAATG TGGACATCAC CCGGGAACCT
    GAAGAAGCCC TGGACACTGT TCCTGCCCAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35628
    Clone ID Related Accession (Same CDS sequence) XM_010821985.2 , XM_010821985.3
    Accession Version XM_010821985.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GTATGCCTAT
    GCCCTGGCCA ATTTCATTCA GTCCCAGGGT ATCAGCTCAC TGAAGGTGTG GACCAGCCAC
    ATGAAGAGGA CTATCCAGAC AGCTGAAGCC TTAGGCATTC CCTATGAGCA ATGGAAGGCC
    CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAAGAACAC
    TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATACC GCTACCGCTA TCCTAAGGGA
    GAGTCTTACG AGGATCTGGT CCAGCGTCTG GAGCCAGTTA TAATGGAGCT AGAACGGCAG
    GAAAATGTAT TGGTGATCTG CCATCAGGCT GTCATGCGGT GCCTCCTGGC CTACTTTCTG
    GACAAGAGCT CAGGGTCTGT CCCCGATCCC CAGTACCTGC AGTCCTCCTT GTTCCTGAGG
    CCTCTCTGCT CTCTTCCTCC CTCCCCACTG GGCTCTCAGC ACTGCCCTCT GAAGAGTGTG
    GGGGAGCAGG ACCCAGATGA GTTGCCTTAT CTCAAGTGCC CTCTGCACAC AGTGCTCAAA
    CTTACACCTG TGGCTTATGG CTGCAAAGTG GAGTCCATCT ACCTGAATGT GGAGGCTGTA
    AACACACATC GGGAGAAGCC TGAGAATGTG GACATCACCC GGGAACCTGA AGAAGCCCTG
    GACACTGTTC CTGCCCACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820287.1
    CDS381..2204
    Translation

    Target ORF information:

    RefSeq Version XM_010821985.2
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821985.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GTATGCCTAT
    GCCCTGGCCA ATTTCATTCA GTCCCAGGGT ATCAGCTCAC TGAAGGTGTG GACCAGCCAC
    ATGAAGAGGA CTATCCAGAC AGCTGAAGCC TTAGGCATTC CCTATGAGCA ATGGAAGGCC
    CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAAGAACAC
    TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATACC GCTACCGCTA TCCTAAGGGA
    GAGTCTTACG AGGATCTGGT CCAGCGTCTG GAGCCAGTTA TAATGGAGCT AGAACGGCAG
    GAAAATGTAT TGGTGATCTG CCATCAGGCT GTCATGCGGT GCCTCCTGGC CTACTTTCTG
    GACAAGAGCT CAGGGTCTGT CCCCGATCCC CAGTACCTGC AGTCCTCCTT GTTCCTGAGG
    CCTCTCTGCT CTCTTCCTCC CTCCCCACTG GGCTCTCAGC ACTGCCCTCT GAAGAGTGTG
    GGGGAGCAGG ACCCAGATGA GTTGCCTTAT CTCAAGTGCC CTCTGCACAC AGTGCTCAAA
    CTTACACCTG TGGCTTATGG CTGCAAAGTG GAGTCCATCT ACCTGAATGT GGAGGCTGTA
    AACACACATC GGGAGAAGCC TGAGAATGTG GACATCACCC GGGAACCTGA AGAAGCCCTG
    GACACTGTTC CTGCCCACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35628
    Clone ID Related Accession (Same CDS sequence) XM_010821985.2 , XM_010821985.3
    Accession Version XM_010821985.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821985.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GTATGCCTAT
    GCCCTGGCCA ATTTCATTCA GTCCCAGGGT ATCAGCTCAC TGAAGGTGTG GACCAGCCAC
    ATGAAGAGGA CTATCCAGAC AGCTGAAGCC TTAGGCATTC CCTATGAGCA ATGGAAGGCC
    CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAAGAACAC
    TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATACC GCTACCGCTA TCCTAAGGGA
    GAGTCTTACG AGGATCTGGT CCAGCGTCTG GAGCCAGTTA TAATGGAGCT AGAACGGCAG
    GAAAATGTAT TGGTGATCTG CCATCAGGCT GTCATGCGGT GCCTCCTGGC CTACTTTCTG
    GACAAGAGCT CAGGGTCTGT CCCCGATCCC CAGTACCTGC AGTCCTCCTT GTTCCTGAGG
    CCTCTCTGCT CTCTTCCTCC CTCCCCACTG GGCTCTCAGC ACTGCCCTCT GAAGAGTGTG
    GGGGAGCAGG ACCCAGATGA GTTGCCTTAT CTCAAGTGCC CTCTGCACAC AGTGCTCAAA
    CTTACACCTG TGGCTTATGG CTGCAAAGTG GAGTCCATCT ACCTGAATGT GGAGGCTGTA
    AACACACATC GGGAGAAGCC TGAGAATGTG GACATCACCC GGGAACCTGA AGAAGCCCTG
    GACACTGTTC CTGCCCACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820287.1
    CDS375..2198
    Translation

    Target ORF information:

    RefSeq Version XM_010821985.3
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821985.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GTATGCCTAT
    GCCCTGGCCA ATTTCATTCA GTCCCAGGGT ATCAGCTCAC TGAAGGTGTG GACCAGCCAC
    ATGAAGAGGA CTATCCAGAC AGCTGAAGCC TTAGGCATTC CCTATGAGCA ATGGAAGGCC
    CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAAGAACAC
    TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATACC GCTACCGCTA TCCTAAGGGA
    GAGTCTTACG AGGATCTGGT CCAGCGTCTG GAGCCAGTTA TAATGGAGCT AGAACGGCAG
    GAAAATGTAT TGGTGATCTG CCATCAGGCT GTCATGCGGT GCCTCCTGGC CTACTTTCTG
    GACAAGAGCT CAGGGTCTGT CCCCGATCCC CAGTACCTGC AGTCCTCCTT GTTCCTGAGG
    CCTCTCTGCT CTCTTCCTCC CTCCCCACTG GGCTCTCAGC ACTGCCCTCT GAAGAGTGTG
    GGGGAGCAGG ACCCAGATGA GTTGCCTTAT CTCAAGTGCC CTCTGCACAC AGTGCTCAAA
    CTTACACCTG TGGCTTATGG CTGCAAAGTG GAGTCCATCT ACCTGAATGT GGAGGCTGTA
    AACACACATC GGGAGAAGCC TGAGAATGTG GACATCACCC GGGAACCTGA AGAAGCCCTG
    GACACTGTTC CTGCCCACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35630
    Clone ID Related Accession (Same CDS sequence) XM_015461707.1 , XM_015461707.2
    Accession Version XM_015461707.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GGGTGTCTGT
    GAGGAGATGA CCTATGAAGA AATCCAAGAA CACTACCCTG AAGAATTTGC ACTGCGAGAC
    CAAGATAAAT ACCGCTACCG CTATCCTAAG GGAGAGTCTT ACGAGGATCT GGTCCAGCGT
    CTGGAGCCAG TTATAATGGA GCTAGAACGG CAGGAAAATG TATTGGTGAT CTGCCATCAG
    GCTGTCATGC GGTGCCTCCT GGCCTACTTT CTGGACAAGA GCTCAGGGTC TGTCCCCGAT
    CCCCAGTACC TGCAGTCCTC CTTGTTCCTG AGGCCTCTCT GCTCTCTTCC TCCCTCCCCA
    CTGGGCTCTC AGCACTGCCC TCTGAAGAGT GTGGGGGAGC AGGACCCAGA TGAGTTGCCT
    TATCTCAAGT GCCCTCTGCA CACAGTGCTC AAACTTACAC CTGTGGCTTA TGGCTGCAAA
    GTGGAGTCCA TCTACCTGAA TGTGGAGGCT GTAAACACAC ATCGGGAGAA GCCTGAGAAT
    GTGGACATCA CCCGGGAACC TGAAGAAGCC CTGGACACTG TTCCTGCCCA CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317193.1
    CDS385..2061
    Translation

    Target ORF information:

    RefSeq Version XM_015461707.1
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GGGTGTCTGT
    GAGGAGATGA CCTATGAAGA AATCCAAGAA CACTACCCTG AAGAATTTGC ACTGCGAGAC
    CAAGATAAAT ACCGCTACCG CTATCCTAAG GGAGAGTCTT ACGAGGATCT GGTCCAGCGT
    CTGGAGCCAG TTATAATGGA GCTAGAACGG CAGGAAAATG TATTGGTGAT CTGCCATCAG
    GCTGTCATGC GGTGCCTCCT GGCCTACTTT CTGGACAAGA GCTCAGGGTC TGTCCCCGAT
    CCCCAGTACC TGCAGTCCTC CTTGTTCCTG AGGCCTCTCT GCTCTCTTCC TCCCTCCCCA
    CTGGGCTCTC AGCACTGCCC TCTGAAGAGT GTGGGGGAGC AGGACCCAGA TGAGTTGCCT
    TATCTCAAGT GCCCTCTGCA CACAGTGCTC AAACTTACAC CTGTGGCTTA TGGCTGCAAA
    GTGGAGTCCA TCTACCTGAA TGTGGAGGCT GTAAACACAC ATCGGGAGAA GCCTGAGAAT
    GTGGACATCA CCCGGGAACC TGAAGAAGCC CTGGACACTG TTCCTGCCCA CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35630
    Clone ID Related Accession (Same CDS sequence) XM_015461707.1 , XM_015461707.2
    Accession Version XM_015461707.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015461707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GGGTGTCTGT
    GAGGAGATGA CCTATGAAGA AATCCAAGAA CACTACCCTG AAGAATTTGC ACTGCGAGAC
    CAAGATAAAT ACCGCTACCG CTATCCTAAG GGAGAGTCTT ACGAGGATCT GGTCCAGCGT
    CTGGAGCCAG TTATAATGGA GCTAGAACGG CAGGAAAATG TATTGGTGAT CTGCCATCAG
    GCTGTCATGC GGTGCCTCCT GGCCTACTTT CTGGACAAGA GCTCAGGGTC TGTCCCCGAT
    CCCCAGTACC TGCAGTCCTC CTTGTTCCTG AGGCCTCTCT GCTCTCTTCC TCCCTCCCCA
    CTGGGCTCTC AGCACTGCCC TCTGAAGAGT GTGGGGGAGC AGGACCCAGA TGAGTTGCCT
    TATCTCAAGT GCCCTCTGCA CACAGTGCTC AAACTTACAC CTGTGGCTTA TGGCTGCAAA
    GTGGAGTCCA TCTACCTGAA TGTGGAGGCT GTAAACACAC ATCGGGAGAA GCCTGAGAAT
    GTGGACATCA CCCGGGAACC TGAAGAAGCC CTGGACACTG TTCCTGCCCA CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317193.1
    CDS379..2055
    Translation

    Target ORF information:

    RefSeq Version XM_015461707.2
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461707.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GGGTGTCTGT
    GAGGAGATGA CCTATGAAGA AATCCAAGAA CACTACCCTG AAGAATTTGC ACTGCGAGAC
    CAAGATAAAT ACCGCTACCG CTATCCTAAG GGAGAGTCTT ACGAGGATCT GGTCCAGCGT
    CTGGAGCCAG TTATAATGGA GCTAGAACGG CAGGAAAATG TATTGGTGAT CTGCCATCAG
    GCTGTCATGC GGTGCCTCCT GGCCTACTTT CTGGACAAGA GCTCAGGGTC TGTCCCCGAT
    CCCCAGTACC TGCAGTCCTC CTTGTTCCTG AGGCCTCTCT GCTCTCTTCC TCCCTCCCCA
    CTGGGCTCTC AGCACTGCCC TCTGAAGAGT GTGGGGGAGC AGGACCCAGA TGAGTTGCCT
    TATCTCAAGT GCCCTCTGCA CACAGTGCTC AAACTTACAC CTGTGGCTTA TGGCTGCAAA
    GTGGAGTCCA TCTACCTGAA TGTGGAGGCT GTAAACACAC ATCGGGAGAA GCCTGAGAAT
    GTGGACATCA CCCGGGAACC TGAAGAAGCC CTGGACACTG TTCCTGCCCA CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35632
    Clone ID Related Accession (Same CDS sequence) XM_005228210.4 , XM_005228210.3
    Accession Version XM_005228210.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005228210.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAGAAA AAGCCTCTAG GAGAACAGCA TCCATACCAC AGTTTACAAA TTCCCCCACA 
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGAAAGACCT ACATCTCAAC AAAGCTCACA
    CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA ATATCGACGA
    GAGGCAGTGA GCTACAAGAA CTACGAATTC TTTCTTCCCG ACAACATGGA GGCCCTACTT
    ATCAGGAAGC AGTGTGCCCT AGCAGCCCTG AAAGATGTTC ATAGCTATCT CAGCCATGAG
    GAAGGTCGTG TTGCAGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATT
    CTGCAGTTTG CTAAAGAACA TGGTTACAAG GTCTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGACGTCA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGTCCTGA TTATATAGAC
    TGTGATCGTG AAAAGGTTCT GGAAGATTTT CTAAAGAGAA TAGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGATGA ACTGGATAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA TCGTGTGCAG GACCATATCC AGAGCCGCAC AGTCTACTAT
    CTCATGAACA TTCACGTCAC ACCCCGCTCC ATCTACCTAT GCCGGCACGG TGAGAGTGAA
    CTCAACCTCA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT
    GCCTATGCCC TGGCCAATTT CATTCAGTCC CAGGGTATCA GCTCACTGAA GGTGTGGACC
    AGCCACATGA AGAGGACTAT CCAGACAGCT GAAGCCTTAG GCATTCCCTA TGAGCAATGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAA
    GAACACTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATACCGCTA CCGCTATCCT
    AAGGGAGAGT CTTACGAGGA TCTGGTCCAG CGTCTGGAGC CAGTTATAAT GGAGCTAGAA
    CGGCAGGAAA ATGTATTGGT GATCTGCCAT CAGGCTGTCA TGCGGTGCCT CCTGGCCTAC
    TTTCTGGACA AGAGCTCAGG GTCTGTCCCC GATCCCCAGT ACCTGCAGTC CTCCTTGTTC
    CTGAGGCCTC TCTGCTCTCT TCCTCCCTCC CCACTGGGCT CTCAGCACTG CCCTCTGAAG
    AGTGTGGGGG AGCAGGACCC AGATGAGTTG CCTTATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTTA CACCTGTGGC TTATGGCTGC AAAGTGGAGT CCATCTACCT GAATGTGGAG
    GCTGTAAACA CACATCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAAGAA
    GCCCTGGACA CTGTTCCTGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228267.1
    CDS215..1684
    Translation

    Target ORF information:

    RefSeq Version XM_005228210.4
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228210.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAGAAA AAGCCTCTAG GAGAACAGCA TCCATACCAC AGTTTACAAA TTCCCCCACA 
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGAAAGACCT ACATCTCAAC AAAGCTCACA
    CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA ATATCGACGA
    GAGGCAGTGA GCTACAAGAA CTACGAATTC TTTCTTCCCG ACAACATGGA GGCCCTACTT
    ATCAGGAAGC AGTGTGCCCT AGCAGCCCTG AAAGATGTTC ATAGCTATCT CAGCCATGAG
    GAAGGTCGTG TTGCAGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATT
    CTGCAGTTTG CTAAAGAACA TGGTTACAAG GTCTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGACGTCA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGTCCTGA TTATATAGAC
    TGTGATCGTG AAAAGGTTCT GGAAGATTTT CTAAAGAGAA TAGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGATGA ACTGGATAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA TCGTGTGCAG GACCATATCC AGAGCCGCAC AGTCTACTAT
    CTCATGAACA TTCACGTCAC ACCCCGCTCC ATCTACCTAT GCCGGCACGG TGAGAGTGAA
    CTCAACCTCA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT
    GCCTATGCCC TGGCCAATTT CATTCAGTCC CAGGGTATCA GCTCACTGAA GGTGTGGACC
    AGCCACATGA AGAGGACTAT CCAGACAGCT GAAGCCTTAG GCATTCCCTA TGAGCAATGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAA
    GAACACTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATACCGCTA CCGCTATCCT
    AAGGGAGAGT CTTACGAGGA TCTGGTCCAG CGTCTGGAGC CAGTTATAAT GGAGCTAGAA
    CGGCAGGAAA ATGTATTGGT GATCTGCCAT CAGGCTGTCA TGCGGTGCCT CCTGGCCTAC
    TTTCTGGACA AGAGCTCAGG GTCTGTCCCC GATCCCCAGT ACCTGCAGTC CTCCTTGTTC
    CTGAGGCCTC TCTGCTCTCT TCCTCCCTCC CCACTGGGCT CTCAGCACTG CCCTCTGAAG
    AGTGTGGGGG AGCAGGACCC AGATGAGTTG CCTTATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTTA CACCTGTGGC TTATGGCTGC AAAGTGGAGT CCATCTACCT GAATGTGGAG
    GCTGTAAACA CACATCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAAGAA
    GCCCTGGACA CTGTTCCTGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35632
    Clone ID Related Accession (Same CDS sequence) XM_005228210.4 , XM_005228210.3
    Accession Version XM_005228210.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228210.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAGAAA AAGCCTCTAG GAGAACAGCA TCCATACCAC AGTTTACAAA TTCCCCCACA 
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGAAAGACCT ACATCTCAAC AAAGCTCACA
    CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA ATATCGACGA
    GAGGCAGTGA GCTACAAGAA CTACGAATTC TTTCTTCCCG ACAACATGGA GGCCCTACTT
    ATCAGGAAGC AGTGTGCCCT AGCAGCCCTG AAAGATGTTC ATAGCTATCT CAGCCATGAG
    GAAGGTCGTG TTGCAGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATT
    CTGCAGTTTG CTAAAGAACA TGGTTACAAG GTCTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGACGTCA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGTCCTGA TTATATAGAC
    TGTGATCGTG AAAAGGTTCT GGAAGATTTT CTAAAGAGAA TAGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGATGA ACTGGATAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA TCGTGTGCAG GACCATATCC AGAGCCGCAC AGTCTACTAT
    CTCATGAACA TTCACGTCAC ACCCCGCTCC ATCTACCTAT GCCGGCACGG TGAGAGTGAA
    CTCAACCTCA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT
    GCCTATGCCC TGGCCAATTT CATTCAGTCC CAGGGTATCA GCTCACTGAA GGTGTGGACC
    AGCCACATGA AGAGGACTAT CCAGACAGCT GAAGCCTTAG GCATTCCCTA TGAGCAATGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAA
    GAACACTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATACCGCTA CCGCTATCCT
    AAGGGAGAGT CTTACGAGGA TCTGGTCCAG CGTCTGGAGC CAGTTATAAT GGAGCTAGAA
    CGGCAGGAAA ATGTATTGGT GATCTGCCAT CAGGCTGTCA TGCGGTGCCT CCTGGCCTAC
    TTTCTGGACA AGAGCTCAGG GTCTGTCCCC GATCCCCAGT ACCTGCAGTC CTCCTTGTTC
    CTGAGGCCTC TCTGCTCTCT TCCTCCCTCC CCACTGGGCT CTCAGCACTG CCCTCTGAAG
    AGTGTGGGGG AGCAGGACCC AGATGAGTTG CCTTATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTTA CACCTGTGGC TTATGGCTGC AAAGTGGAGT CCATCTACCT GAATGTGGAG
    GCTGTAAACA CACATCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAAGAA
    GCCCTGGACA CTGTTCCTGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005228267.1
    CDS222..1691
    Translation

    Target ORF information:

    RefSeq Version XM_005228210.3
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005228210.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAGAAA AAGCCTCTAG GAGAACAGCA TCCATACCAC AGTTTACAAA TTCCCCCACA 
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGAAAGACCT ACATCTCAAC AAAGCTCACA
    CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA ATATCGACGA
    GAGGCAGTGA GCTACAAGAA CTACGAATTC TTTCTTCCCG ACAACATGGA GGCCCTACTT
    ATCAGGAAGC AGTGTGCCCT AGCAGCCCTG AAAGATGTTC ATAGCTATCT CAGCCATGAG
    GAAGGTCGTG TTGCAGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATT
    CTGCAGTTTG CTAAAGAACA TGGTTACAAG GTCTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGACGTCA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGTCCTGA TTATATAGAC
    TGTGATCGTG AAAAGGTTCT GGAAGATTTT CTAAAGAGAA TAGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGATGA ACTGGATAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA TCGTGTGCAG GACCATATCC AGAGCCGCAC AGTCTACTAT
    CTCATGAACA TTCACGTCAC ACCCCGCTCC ATCTACCTAT GCCGGCACGG TGAGAGTGAA
    CTCAACCTCA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT
    GCCTATGCCC TGGCCAATTT CATTCAGTCC CAGGGTATCA GCTCACTGAA GGTGTGGACC
    AGCCACATGA AGAGGACTAT CCAGACAGCT GAAGCCTTAG GCATTCCCTA TGAGCAATGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAA
    GAACACTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATACCGCTA CCGCTATCCT
    AAGGGAGAGT CTTACGAGGA TCTGGTCCAG CGTCTGGAGC CAGTTATAAT GGAGCTAGAA
    CGGCAGGAAA ATGTATTGGT GATCTGCCAT CAGGCTGTCA TGCGGTGCCT CCTGGCCTAC
    TTTCTGGACA AGAGCTCAGG GTCTGTCCCC GATCCCCAGT ACCTGCAGTC CTCCTTGTTC
    CTGAGGCCTC TCTGCTCTCT TCCTCCCTCC CCACTGGGCT CTCAGCACTG CCCTCTGAAG
    AGTGTGGGGG AGCAGGACCC AGATGAGTTG CCTTATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTTA CACCTGTGGC TTATGGCTGC AAAGTGGAGT CCATCTACCT GAATGTGGAG
    GCTGTAAACA CACATCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAAGAA
    GCCCTGGACA CTGTTCCTGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45422
    Clone ID Related Accession (Same CDS sequence) XM_010821986.2 , XM_010821986.3
    Accession Version XM_010821986.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGAAAGACCT ACATCTCAAC AAAGCTCACA 
    CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA ATATCGACGA
    GAGGCAGTGA GCTACAAGAA CTACGAATTC TTTCTTCCCG ACAACATGGA GGCCCTACTT
    ATCAGGAAGC AGTGTGCCCT AGCAGCCCTG AAAGATGTTC ATAGCTATCT CAGCCATGAG
    GAAGGTCGTG TTGCAGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATT
    CTGCAGTTTG CTAAAGAACA TGGTTACAAG GTCTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGACGTCA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGTCCTGA TTATATAGAC
    TGTGATCGTG AAAAGGTTCT GGAAGATTTT CTAAAGAGAA TAGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGATGA ACTGGATAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA TCGTGTGCAG GACCATATCC AGAGCCGCAC AGTCTACTAT
    CTCATGAACA TTCACGTCAC ACCCCGCTCC ATCTACCTAT GCCGGCACGG TGAGAGTGAA
    CTCAACCTCA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT
    GCCTATGCCC TGGCCAATTT CATTCAGTCC CAGGGTATCA GCTCACTGAA GGTGTGGACC
    AGCCACATGA AGAGGACTAT CCAGACAGCT GAAGCCTTAG GCATTCCCTA TGAGCAATGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAA
    GAACACTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATACCGCTA CCGCTATCCT
    AAGGGAGAGT CTTACGAGGA TCTGGTCCAG CGTCTGGAGC CAGTTATAAT GGAGCTAGAA
    CGGCAGGAAA ATGTATTGGT GATCTGCCAT CAGGCTGTCA TGCGGTGCCT CCTGGCCTAC
    TTTCTGGACA AGAGCTCAGG GTCTGTCCCC GATCCCCAGT ACCTGCAGTC CTCCTTGTTC
    CTGAGGCCTC TCTGCTCTCT TCCTCCCTCC CCACTGGGCT CTCAGCACTG CCCTCTGAAG
    AGTGTGGGGG AGCAGGACCC AGATGAGTTG CCTTATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTTA CACCTGTGGC TTATGGCTGC AAAGTGGAGT CCATCTACCT GAATGTGGAG
    GCTGTAAACA CACATCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAAGAA
    GCCCTGGACA CTGTTCCTGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820288.1
    CDS359..1768
    Translation

    Target ORF information:

    RefSeq Version XM_010821986.2
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821986.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGAAAGACCT ACATCTCAAC AAAGCTCACA 
    CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA ATATCGACGA
    GAGGCAGTGA GCTACAAGAA CTACGAATTC TTTCTTCCCG ACAACATGGA GGCCCTACTT
    ATCAGGAAGC AGTGTGCCCT AGCAGCCCTG AAAGATGTTC ATAGCTATCT CAGCCATGAG
    GAAGGTCGTG TTGCAGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATT
    CTGCAGTTTG CTAAAGAACA TGGTTACAAG GTCTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGACGTCA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGTCCTGA TTATATAGAC
    TGTGATCGTG AAAAGGTTCT GGAAGATTTT CTAAAGAGAA TAGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGATGA ACTGGATAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA TCGTGTGCAG GACCATATCC AGAGCCGCAC AGTCTACTAT
    CTCATGAACA TTCACGTCAC ACCCCGCTCC ATCTACCTAT GCCGGCACGG TGAGAGTGAA
    CTCAACCTCA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT
    GCCTATGCCC TGGCCAATTT CATTCAGTCC CAGGGTATCA GCTCACTGAA GGTGTGGACC
    AGCCACATGA AGAGGACTAT CCAGACAGCT GAAGCCTTAG GCATTCCCTA TGAGCAATGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAA
    GAACACTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATACCGCTA CCGCTATCCT
    AAGGGAGAGT CTTACGAGGA TCTGGTCCAG CGTCTGGAGC CAGTTATAAT GGAGCTAGAA
    CGGCAGGAAA ATGTATTGGT GATCTGCCAT CAGGCTGTCA TGCGGTGCCT CCTGGCCTAC
    TTTCTGGACA AGAGCTCAGG GTCTGTCCCC GATCCCCAGT ACCTGCAGTC CTCCTTGTTC
    CTGAGGCCTC TCTGCTCTCT TCCTCCCTCC CCACTGGGCT CTCAGCACTG CCCTCTGAAG
    AGTGTGGGGG AGCAGGACCC AGATGAGTTG CCTTATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTTA CACCTGTGGC TTATGGCTGC AAAGTGGAGT CCATCTACCT GAATGTGGAG
    GCTGTAAACA CACATCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAAGAA
    GCCCTGGACA CTGTTCCTGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45422
    Clone ID Related Accession (Same CDS sequence) XM_010821986.2 , XM_010821986.3
    Accession Version XM_010821986.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1410bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGAAAGACCT ACATCTCAAC AAAGCTCACA 
    CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA ATATCGACGA
    GAGGCAGTGA GCTACAAGAA CTACGAATTC TTTCTTCCCG ACAACATGGA GGCCCTACTT
    ATCAGGAAGC AGTGTGCCCT AGCAGCCCTG AAAGATGTTC ATAGCTATCT CAGCCATGAG
    GAAGGTCGTG TTGCAGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATT
    CTGCAGTTTG CTAAAGAACA TGGTTACAAG GTCTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGACGTCA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGTCCTGA TTATATAGAC
    TGTGATCGTG AAAAGGTTCT GGAAGATTTT CTAAAGAGAA TAGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGATGA ACTGGATAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA TCGTGTGCAG GACCATATCC AGAGCCGCAC AGTCTACTAT
    CTCATGAACA TTCACGTCAC ACCCCGCTCC ATCTACCTAT GCCGGCACGG TGAGAGTGAA
    CTCAACCTCA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT
    GCCTATGCCC TGGCCAATTT CATTCAGTCC CAGGGTATCA GCTCACTGAA GGTGTGGACC
    AGCCACATGA AGAGGACTAT CCAGACAGCT GAAGCCTTAG GCATTCCCTA TGAGCAATGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAA
    GAACACTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATACCGCTA CCGCTATCCT
    AAGGGAGAGT CTTACGAGGA TCTGGTCCAG CGTCTGGAGC CAGTTATAAT GGAGCTAGAA
    CGGCAGGAAA ATGTATTGGT GATCTGCCAT CAGGCTGTCA TGCGGTGCCT CCTGGCCTAC
    TTTCTGGACA AGAGCTCAGG GTCTGTCCCC GATCCCCAGT ACCTGCAGTC CTCCTTGTTC
    CTGAGGCCTC TCTGCTCTCT TCCTCCCTCC CCACTGGGCT CTCAGCACTG CCCTCTGAAG
    AGTGTGGGGG AGCAGGACCC AGATGAGTTG CCTTATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTTA CACCTGTGGC TTATGGCTGC AAAGTGGAGT CCATCTACCT GAATGTGGAG
    GCTGTAAACA CACATCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAAGAA
    GCCCTGGACA CTGTTCCTGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010820288.1
    CDS353..1762
    Translation

    Target ORF information:

    RefSeq Version XM_010821986.3
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010821986.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGAAAGACCT ACATCTCAAC AAAGCTCACA 
    CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGCCA ATATCGACGA
    GAGGCAGTGA GCTACAAGAA CTACGAATTC TTTCTTCCCG ACAACATGGA GGCCCTACTT
    ATCAGGAAGC AGTGTGCCCT AGCAGCCCTG AAAGATGTTC ATAGCTATCT CAGCCATGAG
    GAAGGTCGTG TTGCAGTTTT TGATGCCACC AACACTACCA GAGAACGACG GTCACTGATT
    CTGCAGTTTG CTAAAGAACA TGGTTACAAG GTCTTTTTCA TTGAGTCCAT TTGTAATGAC
    CCTGACGTCA TTGCAGAAAA CATCAGGCAA GTGAAACTTG GCAGTCCTGA TTATATAGAC
    TGTGATCGTG AAAAGGTTCT GGAAGATTTT CTAAAGAGAA TAGAGTGCTA TGAGGTCAAC
    TACCAACCCT TGGATGATGA ACTGGATAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG
    GGCACACGCT ACATGGTGAA TCGTGTGCAG GACCATATCC AGAGCCGCAC AGTCTACTAT
    CTCATGAACA TTCACGTCAC ACCCCGCTCC ATCTACCTAT GCCGGCACGG TGAGAGTGAA
    CTCAACCTCA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT
    GCCTATGCCC TGGCCAATTT CATTCAGTCC CAGGGTATCA GCTCACTGAA GGTGTGGACC
    AGCCACATGA AGAGGACTAT CCAGACAGCT GAAGCCTTAG GCATTCCCTA TGAGCAATGG
    AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAGGAGA TGACCTATGA AGAAATCCAA
    GAACACTACC CTGAAGAATT TGCACTGCGA GACCAAGATA AATACCGCTA CCGCTATCCT
    AAGGGAGAGT CTTACGAGGA TCTGGTCCAG CGTCTGGAGC CAGTTATAAT GGAGCTAGAA
    CGGCAGGAAA ATGTATTGGT GATCTGCCAT CAGGCTGTCA TGCGGTGCCT CCTGGCCTAC
    TTTCTGGACA AGAGCTCAGG GTCTGTCCCC GATCCCCAGT ACCTGCAGTC CTCCTTGTTC
    CTGAGGCCTC TCTGCTCTCT TCCTCCCTCC CCACTGGGCT CTCAGCACTG CCCTCTGAAG
    AGTGTGGGGG AGCAGGACCC AGATGAGTTG CCTTATCTCA AGTGCCCTCT GCACACAGTG
    CTCAAACTTA CACCTGTGGC TTATGGCTGC AAAGTGGAGT CCATCTACCT GAATGTGGAG
    GCTGTAAACA CACATCGGGA GAAGCCTGAG AATGTGGACA TCACCCGGGA ACCTGAAGAA
    GCCCTGGACA CTGTTCCTGC CCACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238263
    Clone ID Related Accession (Same CDS sequence) XM_024987771.1
    Accession Version XM_024987771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GTATGCCTAT
    GCCCTGGCCA ATTTCATTCA GTCCCAGGGT ATCAGCTCAC TGAAGGTGTG GACCAGCCAC
    ATGAAGAGGA CTATCCAGAC AGCTGAAGCC TTAGGCATTC CCTATGAGCA ATGGAAGGCC
    CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAAGAACAC
    TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATACC GCTACCGCTA TCCTAAGGGA
    GAGTCTTACG AGGATCTGGT CCAGCGTCTG GAGCCAGTTA TAATGGAGCT AGAACGGCAG
    GAAAATGTAT TGGTGATCTG CCATCAGGCT GTCATGCGGT GCCTCCTGGC CTACTTTCTG
    GACAAGAGCT CAGATGAGTT GCCTTATCTC AAGTGCCCTC TGCACACAGT GCTCAAACTT
    ACACCTGTGG CTTATGGCTG CAAAGTGGAG TCCATCTACC TGAATGTGGA GGCTGTAAAC
    ACACATCGGG AGAAGCCTGA GAATGTGGAC ATCACCCGGG AACCTGAAGA AGCCCTGGAC
    ACTGTTCCTG CCCACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843539.1
    CDS372..2072
    Translation

    Target ORF information:

    RefSeq Version XM_024987771.1
    Organism Bos taurus(cattle)
    Definition Bos taurus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTACACAG CCCTTGGAGT TCAGAGGCCT CTGCTAACCT CTGCCCAACC TCTCCTCTGC 
    ACCCAAGCCC TCGATGGTAC ATACAAGAGC AGAGGAAGGC AAGGACTAGG AGACAAGAGA
    GAGGCTGGAG ACAGGCTACA AAAGGGCTCA GAACCCCAGC ACACCTCCCT GTCCCAACTC
    TGTCCACCAC CTGCTGTGGC CACAGCAAAA GAAGAGAGAG CTATAAGACA GAAAGTAAGG
    CCGGGTACCT TGTGGACAGT AGTGTCGTTA GCTGTGAGGC CCAAGATGTC TCAAGAGATG
    GGAGAGCTCA CCCAAACCAG ATTGCAGAAG ATCTGGATTC CACACAACAA CGGCAACAGC
    AGGCTTCAAC GGAGGAGGGG CTCATCCATA CCACAGTTTA CAAATTCCCC CACAATGGTG
    ATCATGGTGG GTTTACCAGC TCGAGGAAAG ACCTACATCT CAACAAAGCT CACACGCTAT
    CTCAACTGGA TAGGAACACC AACTAAAGTG TTTAATTTAG GCCAATATCG ACGAGAGGCA
    GTGAGCTACA AGAACTACGA ATTCTTTCTT CCCGACAACA TGGAGGCCCT ACTTATCAGG
    AAGCAGTGTG CCCTAGCAGC CCTGAAAGAT GTTCATAGCT ATCTCAGCCA TGAGGAAGGT
    CGTGTTGCAG TTTTTGATGC CACCAACACT ACCAGAGAAC GACGGTCACT GATTCTGCAG
    TTTGCTAAAG AACATGGTTA CAAGGTCTTT TTCATTGAGT CCATTTGTAA TGACCCTGAC
    GTCATTGCAG AAAACATCAG GCAAGTGAAA CTTGGCAGTC CTGATTATAT AGACTGTGAT
    CGTGAAAAGG TTCTGGAAGA TTTTCTAAAG AGAATAGAGT GCTATGAGGT CAACTACCAA
    CCCTTGGATG ATGAACTGGA TAGCCACCTG TCCTACATCA AGATCTTCGA CGTGGGCACA
    CGCTACATGG TGAATCGTGT GCAGGACCAT ATCCAGAGCC GCACAGTCTA CTATCTCATG
    AACATTCACG TCACACCCCG CTCCATCTAC CTATGCCGGC ACGGTGAGAG TGAACTCAAC
    CTCAGAGGCC GCATTGGAGG TGACTCTGGC CTCTCAGCTC GGGGCAAGCA GTATGCCTAT
    GCCCTGGCCA ATTTCATTCA GTCCCAGGGT ATCAGCTCAC TGAAGGTGTG GACCAGCCAC
    ATGAAGAGGA CTATCCAGAC AGCTGAAGCC TTAGGCATTC CCTATGAGCA ATGGAAGGCC
    CTGAATGAGA TTGATGCGGG TGTCTGTGAG GAGATGACCT ATGAAGAAAT CCAAGAACAC
    TACCCTGAAG AATTTGCACT GCGAGACCAA GATAAATACC GCTACCGCTA TCCTAAGGGA
    GAGTCTTACG AGGATCTGGT CCAGCGTCTG GAGCCAGTTA TAATGGAGCT AGAACGGCAG
    GAAAATGTAT TGGTGATCTG CCATCAGGCT GTCATGCGGT GCCTCCTGGC CTACTTTCTG
    GACAAGAGCT CAGATGAGTT GCCTTATCTC AAGTGCCCTC TGCACACAGT GCTCAAACTT
    ACACCTGTGG CTTATGGCTG CAAAGTGGAG TCCATCTACC TGAATGTGGA GGCTGTAAAC
    ACACATCGGG AGAAGCCTGA GAATGTGGAC ATCACCCGGG AACCTGAAGA AGCCCTGGAC
    ACTGTTCCTG CCCACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.