PABPN1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PABPN1
Entrez Gene ID	282298
Full Name	poly(A) binding protein nuclear 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024997278.1	XP_024853046.1	polyadenylate-binding protein 2 isoform X1
NM_174569.2	NP_776994.1	polyadenylate-binding protein 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Pabp2	NP_724648.1
Caenorhabditis elegans (roundworm)	pabp-2	NP_492504.1
Oryza sativa (rice)	Os06g0219600	NP_001057163.1
Homo sapiens (human)	PABPN1	NP_004634.1
Arabidopsis thaliana (thale cress)	AT5G65260	NP_201329.1
Arabidopsis thaliana (thale cress)	AT5G10350	NP_196597.1
Pan troglodytes (chimpanzee)	PABPN1	XP_001162168.1
Canis lupus familiaris (dog)	PABPN1	NP_001123909.2
Rattus norvegicus (Norway rat)	Pabpn1	NP_001128480.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005117	XP_313998.3
Oryza sativa (rice)	Os02g0757900	NP_001048174.1
Bos taurus (cattle)	PABPN1	NP_776994.1
Mus musculus (house mouse)	Pabpn1	NP_062275.1

Gene Ontology
Bibliography

Related articles in PubMed

Poly(A) tail length is controlled by the nuclear poly(A)-binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor.
K?hn U, G?ndel M, Knoth A, Kerwitz Y, R?del S, Wahle E
The Journal of biological chemistry284(34)22803-14(2009 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Promiscuous modification of the nuclear poly(A)-binding protein by multiple protein-arginine methyltransferases does not affect the aggregation behavior.
Fronz K, Otto S, K?lbel K, K?hn U, Friedrich H, Schierhorn A, Beck-Sickinger AG, Ostareck-Lederer A, Wahle E
The Journal of biological chemistry283(29)20408-20(2008 Jul)

Trinucleotide expansions leading to an extended poly-L-alanine segment in the poly (A) binding protein PABPN1 cause fibril formation.
Scheuermann T, Schulz B, Blume A, Wahle E, Rudolph R, Schwarz E
Protein science : a publication of the Protein Society12(12)2685-92(2003 Dec)

Stimulation of poly(A) polymerase through a direct interaction with the nuclear poly(A) binding protein allosterically regulated by RNA.
Kerwitz Y, K?hn U, Lilie H, Knoth A, Scheuermann T, Friedrich H, Schwarz E, Wahle E
The EMBO journal22(14)3705-14(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PABPN1 measures the length of the tail and is responsible for disrupting the CPSF-poly(A) polymerase interaction.
Title: Poly(A) tail length is controlled by the nuclear poly(A)-binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor.

C-terminal domain containing the methylated arginine residues is known to promote PAPBN1 self-association, and arginine methylation has been reported to inhibit self-association of an orthologous protein
Title: Promiscuous modification of the nuclear poly(A)-binding protein by multiple protein-arginine methyltransferases does not affect the aggregation behavior.

fibril formation from trinucleotide expansion
Title: Trinucleotide expansions leading to an extended poly-L-alanine segment in the poly (A) binding protein PABPN1 cause fibril formation.

The following PABPN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PABPN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa219412	XM_024997278.1 Latest version!	Bos taurus poly(A) binding protein nuclear 1 (PABPN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OBa41429	NM_174569.2 Latest version!	Bos taurus poly(A) binding protein nuclear 1 (PABPN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PABPN1 ( XM_024997278.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853046.1 Bos taurus poly(A) binding protein nuclear 1 (PABPN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219412
Clone ID Related Accession (Same CDS sequence)	XM_024997278.1
Accession Version	XM_024997278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	polyadenylate-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG AAGCTGAGAA GCTAAAGGAG CTACAGAACG AGGTAGAGAA GCAGATGAAT 
ATGAGTCCAC CTCCGGGCAA TGCTGGCCCA GTGATCATGT CCATTGAGGA GAAGATGGAG 
GCTGATGCCC GTTCCATCTA TGTTGGCAAT GTGGACTATG GTGCAACAGC AGAAGAGCTA 
GAAGCACACT TTCATGGCTG TGGTTCAGTC AACCGCGTAA CTATACTCTG TGACAAATTT 
AGTGGCCATC CGAAAGGGTT TGCGTATATA GAGTTCTCAG ACAAAGAGTC AGTGAGGACT 
TCCCTGGCCT TAGATGAATC CTTATTTAGA GGAAGACAGA TCAAGGTGAT CCCTAAACGA 
ACCAACAGAC CAGGCATCAG CACAACAGAC CGAGGCTTCC CACGAGCCCG ATACCGTGCC 
CGAACCACCA ACTACAACAG TTCCCGCTCT CGATTCTACA GTGGTTTTAA CAGCAGGCCC 
CGGGGTCGCG TCTACAGGGG CCGGGCTAGA GCGACATCAT GGTATTCCCC TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853046.1
CDS	153..689
Translation	MEEEAEKLKELQNEVEKQMNMSPPPGNAGPVIMSIEEKMEADARSIYVGNVDYGATAEELEAHFHGCGSVNRVTILCDKFSGHPKGFAYIEFSDKESVRTSLALDESLFRGRQIKVIPKRTNRPGISTTDRGFPRARYRARTTNYNSSRSRFYSGFNSRPRGRVYRGRARATSWYSPY

Target ORF information:

RefSeq Version	XM_024997278.1
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(A) binding protein nuclear 1 (PABPN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997278.1

ORF Insert Sequence:

ATGGAGGAAG AAGCTGAGAA GCTAAAGGAG CTACAGAACG AGGTAGAGAA GCAGATGAAT 
ATGAGTCCAC CTCCGGGCAA TGCTGGCCCA GTGATCATGT CCATTGAGGA GAAGATGGAG 
GCTGATGCCC GTTCCATCTA TGTTGGCAAT GTGGACTATG GTGCAACAGC AGAAGAGCTA 
GAAGCACACT TTCATGGCTG TGGTTCAGTC AACCGCGTAA CTATACTCTG TGACAAATTT 
AGTGGCCATC CGAAAGGGTT TGCGTATATA GAGTTCTCAG ACAAAGAGTC AGTGAGGACT 
TCCCTGGCCT TAGATGAATC CTTATTTAGA GGAAGACAGA TCAAGGTGAT CCCTAAACGA 
ACCAACAGAC CAGGCATCAG CACAACAGAC CGAGGCTTCC CACGAGCCCG ATACCGTGCC 
CGAACCACCA ACTACAACAG TTCCCGCTCT CGATTCTACA GTGGTTTTAA CAGCAGGCCC 
CGGGGTCGCG TCTACAGGGG CCGGGCTAGA GCGACATCAT GGTATTCCCC TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PABPN1 ( NM_174569.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776994.1 Bos taurus poly(A) binding protein nuclear 1 (PABPN1), mRNA.

Price & Availability↑

CloneID	OBa41429
Clone ID Related Accession (Same CDS sequence)	NM_174569.2
Accession Version	NM_174569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	polyadenylate-binding protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X89969.1. On Jun 3, 2003 this sequence version replaced NM_174569.1. ##Evidence-Data-START## Transcript exon combination :: X89969.1, BC133558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG CGGCGGCGGC GGCAGCAGCA GCGGGGGCTG CGGGCGGTCG GGGCTCCGGG 
CCGGGGCGGC GGCGCCATCT TGTGCCCGGG GCCGGTGGGG AGGCCGGGGA GGGGGCCCCG 
GGGGGCGCAG GGGACTACGG GAACGGCTTG GAGTCTGAGG AACTGGAGCC TGAGGAGCTG 
CTGCTGGAGC CCGAGCCGGA GCCCGAGCCC GAAGAGGAGC CGCCCCGGCC CCGCGCCCCC 
CCGGGAGCTC CGGGCCCTGG GCCTGGCTCG GGAGCCCCCG GCAATCAGGA GGAGGAGGAG 
GAGTCGGGAC TGGTCGAGGG TGACCCGGGG GACGGCGCCA TTGAGGACCC GGAGCTGGAA 
GCGATCAAAG CTCGAGTTAG GGAGATGGAG GAAGAAGCTG AGAAGCTAAA GGAGCTACAG 
AACGAGGTAG AGAAGCAGAT GAATATGAGT CCACCTCCGG GCAATGCTGG CCCAGTGATC 
ATGTCCATTG AGGAGAAGAT GGAGGCTGAT GCCCGTTCCA TCTATGTTGG CAATGTGGAC 
TATGGTGCAA CAGCAGAAGA GCTAGAAGCA CACTTTCATG GCTGTGGTTC AGTCAACCGC 
GTAACTATAC TCTGTGACAA ATTTAGTGGC CATCCGAAAG GGTTTGCGTA TATAGAGTTC 
TCAGACAAAG AGTCAGTGAG GACTTCCCTG GCCTTAGATG AATCCTTATT TAGAGGAAGA 
CAGATCAAGG TGATCCCTAA ACGAACCAAC AGACCAGGCA TCAGCACAAC AGACCGAGGC 
TTCCCACGAG CCCGATACCG TGCCCGAACC ACCAACTACA ACAGTTCCCG CTCTCGATTC 
TACAGTGGTT TTAACAGCAG GCCCCGGGGT CGCGTCTACA GGGGCCGGGC TAGAGCGACA 
TCATGGTATT CCCCTTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776994.1
CDS	2096..3016
Translation	MAAAAAAAAAAGAAGGRGSGPGRRRHLVPGAGGEAGEGAPGGAGDYGNGLESEELEPEELLLEPEPEPEPEEEPPRPRAPPGAPGPGPGSGAPGNQEEEEESGLVEGDPGDGAIEDPELEAIKARVREMEEEAEKLKELQNEVEKQMNMSPPPGNAGPVIMSIEEKMEADARSIYVGNVDYGATAEELEAHFHGCGSVNRVTILCDKFSGHPKGFAYIEFSDKESVRTSLALDESLFRGRQIKVIPKRTNRPGISTTDRGFPRARYRARTTNYNSSRSRFYSGFNSRPRGRVYRGRARATSWYSPY

Target ORF information:

RefSeq Version	NM_174569.2
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(A) binding protein nuclear 1 (PABPN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174569.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCGGC GGCAGCAGCA GCGGGGGCTG CGGGCGGTCG GGGCTCCGGG 
CCGGGGCGGC GGCGCCATCT TGTGCCCGGG GCCGGTGGGG AGGCCGGGGA GGGGGCCCCG 
GGGGGCGCAG GGGACTACGG GAACGGCTTG GAGTCTGAGG AACTGGAGCC TGAGGAGCTG 
CTGCTGGAGC CCGAGCCGGA GCCCGAGCCC GAAGAGGAGC CGCCCCGGCC CCGCGCCCCC 
CCGGGAGCTC CGGGCCCTGG GCCTGGCTCG GGAGCCCCCG GCAATCAGGA GGAGGAGGAG 
GAGTCGGGAC TGGTCGAGGG TGACCCGGGG GACGGCGCCA TTGAGGACCC GGAGCTGGAA 
GCGATCAAAG CTCGAGTTAG GGAGATGGAG GAAGAAGCTG AGAAGCTAAA GGAGCTACAG 
AACGAGGTAG AGAAGCAGAT GAATATGAGT CCACCTCCGG GCAATGCTGG CCCAGTGATC 
ATGTCCATTG AGGAGAAGAT GGAGGCTGAT GCCCGTTCCA TCTATGTTGG CAATGTGGAC 
TATGGTGCAA CAGCAGAAGA GCTAGAAGCA CACTTTCATG GCTGTGGTTC AGTCAACCGC 
GTAACTATAC TCTGTGACAA ATTTAGTGGC CATCCGAAAG GGTTTGCGTA TATAGAGTTC 
TCAGACAAAG AGTCAGTGAG GACTTCCCTG GCCTTAGATG AATCCTTATT TAGAGGAAGA 
CAGATCAAGG TGATCCCTAA ACGAACCAAC AGACCAGGCA TCAGCACAAC AGACCGAGGC 
TTCCCACGAG CCCGATACCG TGCCCGAACC ACCAACTACA ACAGTTCCCG CTCTCGATTC 
TACAGTGGTT TTAACAGCAG GCCCCGGGGT CGCGTCTACA GGGGCCGGGC TAGAGCGACA 
TCATGGTATT CCCCTTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Poly(A) tail length is controlled by the nuclear poly(A)-binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor.
The Journal of biological chemistry284(34)22803-14(2009 Aug)
K?hn U,G?ndel M,Knoth A,Kerwitz Y,R?del S,Wahle E

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Promiscuous modification of the nuclear poly(A)-binding protein by multiple protein-arginine methyltransferases does not affect the aggregation behavior.
The Journal of biological chemistry283(29)20408-20(2008 Jul)
Fronz K,Otto S,K?lbel K,K?hn U,Friedrich H,Schierhorn A,Beck-Sickinger AG,Ostareck-Lederer A,Wahle E

Trinucleotide expansions leading to an extended poly-L-alanine segment in the poly (A) binding protein PABPN1 cause fibril formation.
Protein science : a publication of the Protein Society12(12)2685-92(2003 Dec)
Scheuermann T,Schulz B,Blume A,Wahle E,Rudolph R,Schwarz E

Stimulation of poly(A) polymerase through a direct interaction with the nuclear poly(A) binding protein allosterically regulated by RNA.
The EMBO journal22(14)3705-14(2003 Jul)
Kerwitz Y,K?hn U,Lilie H,Knoth A,Scheuermann T,Friedrich H,Schwarz E,Wahle E

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PABPN1 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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