COL6A2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL6A2
Entrez Gene ID	282194
Full Name	collagen type VI alpha 2 chain
General protein information	Preferred Names collagen type VI alpha 2 chain Names collagen alpha-2(VI) chain collagen, type VI, alpha 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005202053.4	XP_005202110.1	collagen alpha-2(VI) chain isoform X1
XM_010801673.3	XP_010799975.1	collagen alpha-2(VI) chain isoform X2
NM_001075126.1	NP_001068594.1	collagen alpha-2(VI) chain precursor
XM_010801673.2	XP_010799975.1	collagen alpha-2(VI) chain isoform X2
XM_005202053.3	XP_005202110.1	collagen alpha-2(VI) chain isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following COL6A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL6A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa218609	XM_010801673.3 Latest version!	Bos taurus collagen type VI alpha 2 chain (COL6A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa218608	XM_005202053.4 Latest version!	Bos taurus collagen type VI alpha 2 chain (COL6A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa38197	NM_001075126.1 Latest version!	Bos taurus collagen type VI alpha 2 chain (COL6A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa16124	XM_005202053.3 Latest version!	Bos taurus collagen type VI alpha 2 (COL6A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa45392	XM_010801673.2 Latest version!	Bos taurus collagen type VI alpha 2 (COL6A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL6A2 ( XM_010801673.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799975.1 Bos taurus collagen type VI alpha 2 chain (COL6A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa218609
Clone ID Related Accession (Same CDS sequence)	XM_010801673.3
Accession Version	XM_010801673.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-2(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGGC GCAAGGGTGC TGCCAAGATG CTCCGCAGCC CCTGCTCTGC CCTCCTGCTC 
TGGGGGCTCC TGGGGGCCGT CCATGCTCAG CAGCAGGAGG TCATCAGCCC TGGCCCCTCG 
GACAGGAATA GCTGCCCTGA GAAGGCCGAC TGCCCGGTCC ACGTGTACTT CGTGCTGGAC 
ACGTCGGAGA GCATCACCAT GCAGTCCCCC ACTGACAGCC TGCTCTACCA CATGCAGCAG 
TTCGTGCTGC AGTTCATCAG CCAACTGCAG GATGAGCTCT ACCTGGACCA GGTGGCCCTG 
AGCTGGCGCT ACGGCGGCCT GCACTTCTCC GATCTGGTAG AGGTGTTCAG CCCGCCGGGC 
AGCGACCGGG CCTCCTTCAC CAAGAGCCTG CAGAGCATCA GCTCCTTCCG CCGGGGCACC 
TTCACCGACT GCATGCTGGC CAACATGACC CAGGAGGTCC GGCGGCATGT GGGCAAGGGC 
GTGGTCAACT TCGCGGTGGT CATCACCGAC GGTCACGTGA CCGGCAGCCC CTGCGGGGGC 
ATCAAGCTGC AGGCAGAGAG GGCCCGCGAG GAGGGCATCC GGCTGTTCGC GGTGCCGCCC 
AACCTGAAGC TGAATGAGCA GGGCCTGCGG GACATTGCCA ACACACCCCA CGAGCTCTAC 
CGGAACAACT ACGCGACCAT GCGGCCGGAC TCCACTGAGA TCGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TATGGAGAGT GCTACAAGGT CAGCTGTCTG 
GAGATCCCTG GGCCCCCTGG CCCCAAGGGC TACCGCGGAC AGAAGGGTGC CAAGGGCAAC 
ATGGGTGAGC CTGGAGAGCC TGGACAGAAG GGGCGACAGG GAGATCCAGG CATCGAAGGC 
CCCATTGGAT TCCCTGGACC CAAGGGTGTT CCTGGTTTCA AAGGCGAGAA GGGCGAGTTT 
GGAGCCGACG GGCGGAAGGG TGCCCCGGGC CTGGCGGGCA AGAACGGGAC AGATGGGCAG 
AAGGGCAAGC TGGGGCGCAT TGGACCTCCT GGCTGCAAGG GAGACACCGG GGATCGGGGC 
CCTGATGGCT ACGTGGGGGA AGCCGGCAGC CCTGGGGAGC GAGGAGACCA AGGCTCAAAG 
GGGGACCCTG GCCGCCCAGG ACGCAGAGGC CCCCCCGGGG AGAATGGGGC AAAAGGAAGC 
AAGGGCTATC AAGGCAACAA CGGAGCCCCA GGAAGTCCTG GCCTGAAAGG AGCCAAGGGC 
GGGCCTGGGC CCCGAGGACC CAAAGGCGAG CCTGGGCGCA GGGGAGACCC TGGAACCAAA 
GGCGGCCCAG GCAGTGATGG CCCCAAGGGT GAGAAGGGGG ACCCTGGTCC TGAGGGGCCC 
CGGGGCCTGG CTGGAGAGGT TGGCAACAAA GGAGCCAAGG GAGATCGTGG TTTGCCTGGA 
CCCAGAGGTC CCCAGGGGAC TGTCGGAGAG CCTGGGAAAC AGGGATCTCG GGGAGACCCT 
GGTGATGCTG GTCCCCGCGG AGATTCGGGA CAGCCTGGCC CCAAGGGAGA CCCTGGCAGG 
CCTGGATTCA GCTATCCTGG ACCCCGAGGA GCACCTGGAG ACAAGGGTGA GCCAGGCCCC 
CCCGGCCCCG AGGGGGGCAG AGGTGACTTT GGCTCCAAAG GAGAACCTGG GAGGAAAGGC 
CAGAAGGGAG AGCCTGCAGA TCCTGGTCCC CCTGGTGAGC CTGGCCCACG AGGGCAGAGA 
GGAGCCCCAG GACCTGAGGG AGAGCCTGGC CCCCCAGGAG ACCCCGGCCT CACGGAATGC 
GACGTCATGA CCTACGTGCG GGAGACCTGC GGGTGCTGTG ACTGTGAGAA GCGCTGTGGC 
GCCTTGGATG TGGTGTTCGT CATCGACAGC TCTGAGAGCA TCGGCTACAC CAACTTCACC 
CTGGAGAAGA ACTTCGTCAT CAACGTGGTC AACAGGCTGG GAGCCATCGC CAAGGACCCC 
AAGTCGGAGA CGGGGACCCG CGTGGGCGTC GTGCAGTACA GCCACGAGGG CACCTTCGAA 
GCCATCCAGC TGGACGACGA GCGCATCGAC TCGCTGTCCA GCTTCAAAGA GGCCGTCAAG 
AACCTTGAGT GGATTGCTGG TGGCACCTGG ACGCCCTCGG CCCTCAAGTT CGCCTACAAC 
AAGCTCATCA AGGAGAGCCG GCGCCAGAAG ACCCGCGTGT TCGCGGTGGT CATCACAGAC 
GGCCGCCACG ACCCCCGGGA CGACGACCTC AACCTGCGGG CGCTGTGCAA CCACGAAGTG 
ACGGTGACGG CCATCGGCAT CGGGGACATG TTCCACGAGA AGCACGAGAG CGAGAACCTG 
TACTCCATTG CCTGCGACAA GCCGCAGCAG GTGCGCAACA TGACACTCTT CTCCGACCTG 
GTGGCCGAGA AGTTCATCGA CGACATGGAG GACGTGCTGT GCCCGGATCC CCAGATTGTG 
TGCCCGGATC TTCCCTGCCA AACAGTAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799975.1
CDS	122..2614
Translation	MLRRKGAAKMLRSPCSALLLWGLLGAVHAQQQEVISPGPSDRNSCPEKADCPVHVYFVLDTSESITMQSPTDSLLYHMQQFVLQFISQLQDELYLDQVALSWRYGGLHFSDLVEVFSPPGSDRASFTKSLQSISSFRRGTFTDCMLANMTQEVRRHVGKGVVNFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVPPNLKLNEQGLRDIANTPHELYRNNYATMRPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPPGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDTGDRGPDGYVGEAGSPGERGDQGSKGDPGRPGRRGPPGENGAKGSKGYQGNNGAPGSPGLKGAKGGPGPRGPKGEPGRRGDPGTKGGPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGTVGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGDKGEPGPPGPEGGRGDFGSKGEPGRKGQKGEPADPGPPGEPGPRGQRGAPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYNKLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCNHEVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTVN

Target ORF information:

RefSeq Version	XM_010801673.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 2 chain (COL6A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801673.3

ORF Insert Sequence:

ATGTTGAGGC GCAAGGGTGC TGCCAAGATG CTCCGCAGCC CCTGCTCTGC CCTCCTGCTC 
TGGGGGCTCC TGGGGGCCGT CCATGCTCAG CAGCAGGAGG TCATCAGCCC TGGCCCCTCG 
GACAGGAATA GCTGCCCTGA GAAGGCCGAC TGCCCGGTCC ACGTGTACTT CGTGCTGGAC 
ACGTCGGAGA GCATCACCAT GCAGTCCCCC ACTGACAGCC TGCTCTACCA CATGCAGCAG 
TTCGTGCTGC AGTTCATCAG CCAACTGCAG GATGAGCTCT ACCTGGACCA GGTGGCCCTG 
AGCTGGCGCT ACGGCGGCCT GCACTTCTCC GATCTGGTAG AGGTGTTCAG CCCGCCGGGC 
AGCGACCGGG CCTCCTTCAC CAAGAGCCTG CAGAGCATCA GCTCCTTCCG CCGGGGCACC 
TTCACCGACT GCATGCTGGC CAACATGACC CAGGAGGTCC GGCGGCATGT GGGCAAGGGC 
GTGGTCAACT TCGCGGTGGT CATCACCGAC GGTCACGTGA CCGGCAGCCC CTGCGGGGGC 
ATCAAGCTGC AGGCAGAGAG GGCCCGCGAG GAGGGCATCC GGCTGTTCGC GGTGCCGCCC 
AACCTGAAGC TGAATGAGCA GGGCCTGCGG GACATTGCCA ACACACCCCA CGAGCTCTAC 
CGGAACAACT ACGCGACCAT GCGGCCGGAC TCCACTGAGA TCGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TATGGAGAGT GCTACAAGGT CAGCTGTCTG 
GAGATCCCTG GGCCCCCTGG CCCCAAGGGC TACCGCGGAC AGAAGGGTGC CAAGGGCAAC 
ATGGGTGAGC CTGGAGAGCC TGGACAGAAG GGGCGACAGG GAGATCCAGG CATCGAAGGC 
CCCATTGGAT TCCCTGGACC CAAGGGTGTT CCTGGTTTCA AAGGCGAGAA GGGCGAGTTT 
GGAGCCGACG GGCGGAAGGG TGCCCCGGGC CTGGCGGGCA AGAACGGGAC AGATGGGCAG 
AAGGGCAAGC TGGGGCGCAT TGGACCTCCT GGCTGCAAGG GAGACACCGG GGATCGGGGC 
CCTGATGGCT ACGTGGGGGA AGCCGGCAGC CCTGGGGAGC GAGGAGACCA AGGCTCAAAG 
GGGGACCCTG GCCGCCCAGG ACGCAGAGGC CCCCCCGGGG AGAATGGGGC AAAAGGAAGC 
AAGGGCTATC AAGGCAACAA CGGAGCCCCA GGAAGTCCTG GCCTGAAAGG AGCCAAGGGC 
GGGCCTGGGC CCCGAGGACC CAAAGGCGAG CCTGGGCGCA GGGGAGACCC TGGAACCAAA 
GGCGGCCCAG GCAGTGATGG CCCCAAGGGT GAGAAGGGGG ACCCTGGTCC TGAGGGGCCC 
CGGGGCCTGG CTGGAGAGGT TGGCAACAAA GGAGCCAAGG GAGATCGTGG TTTGCCTGGA 
CCCAGAGGTC CCCAGGGGAC TGTCGGAGAG CCTGGGAAAC AGGGATCTCG GGGAGACCCT 
GGTGATGCTG GTCCCCGCGG AGATTCGGGA CAGCCTGGCC CCAAGGGAGA CCCTGGCAGG 
CCTGGATTCA GCTATCCTGG ACCCCGAGGA GCACCTGGAG ACAAGGGTGA GCCAGGCCCC 
CCCGGCCCCG AGGGGGGCAG AGGTGACTTT GGCTCCAAAG GAGAACCTGG GAGGAAAGGC 
CAGAAGGGAG AGCCTGCAGA TCCTGGTCCC CCTGGTGAGC CTGGCCCACG AGGGCAGAGA 
GGAGCCCCAG GACCTGAGGG AGAGCCTGGC CCCCCAGGAG ACCCCGGCCT CACGGAATGC 
GACGTCATGA CCTACGTGCG GGAGACCTGC GGGTGCTGTG ACTGTGAGAA GCGCTGTGGC 
GCCTTGGATG TGGTGTTCGT CATCGACAGC TCTGAGAGCA TCGGCTACAC CAACTTCACC 
CTGGAGAAGA ACTTCGTCAT CAACGTGGTC AACAGGCTGG GAGCCATCGC CAAGGACCCC 
AAGTCGGAGA CGGGGACCCG CGTGGGCGTC GTGCAGTACA GCCACGAGGG CACCTTCGAA 
GCCATCCAGC TGGACGACGA GCGCATCGAC TCGCTGTCCA GCTTCAAAGA GGCCGTCAAG 
AACCTTGAGT GGATTGCTGG TGGCACCTGG ACGCCCTCGG CCCTCAAGTT CGCCTACAAC 
AAGCTCATCA AGGAGAGCCG GCGCCAGAAG ACCCGCGTGT TCGCGGTGGT CATCACAGAC 
GGCCGCCACG ACCCCCGGGA CGACGACCTC AACCTGCGGG CGCTGTGCAA CCACGAAGTG 
ACGGTGACGG CCATCGGCAT CGGGGACATG TTCCACGAGA AGCACGAGAG CGAGAACCTG 
TACTCCATTG CCTGCGACAA GCCGCAGCAG GTGCGCAACA TGACACTCTT CTCCGACCTG 
GTGGCCGAGA AGTTCATCGA CGACATGGAG GACGTGCTGT GCCCGGATCC CCAGATTGTG 
TGCCCGGATC TTCCCTGCCA AACAGTAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( XM_005202053.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202110.1 Bos taurus collagen type VI alpha 2 chain (COL6A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa218608
Clone ID Related Accession (Same CDS sequence)	XM_005202053.4
Accession Version	XM_005202053.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-2(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202053.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGGC GCAAGGGTGC TGCCAAGATG CTCCGCAGCC CCTGCTCTGC CCTCCTGCTC 
TGGGGGCTCC TGGGGGCCGT CCATGCTCAG CAGCAGGAGG TCATCAGCCC TGGCCCCTCG 
GACAGGAATA GCTGCCCTGA GAAGGCCGAC TGCCCGGTCC ACGTGTACTT CGTGCTGGAC 
ACGTCGGAGA GCATCACCAT GCAGTCCCCC ACTGACAGCC TGCTCTACCA CATGCAGCAG 
TTCGTGCTGC AGTTCATCAG CCAACTGCAG GATGAGCTCT ACCTGGACCA GGTGGCCCTG 
AGCTGGCGCT ACGGCGGCCT GCACTTCTCC GATCTGGTAG AGGTGTTCAG CCCGCCGGGC 
AGCGACCGGG CCTCCTTCAC CAAGAGCCTG CAGAGCATCA GCTCCTTCCG CCGGGGCACC 
TTCACCGACT GCATGCTGGC CAACATGACC CAGGAGGTCC GGCGGCATGT GGGCAAGGGC 
GTGGTCAACT TCGCGGTGGT CATCACCGAC GGTCACGTGA CCGGCAGCCC CTGCGGGGGC 
ATCAAGCTGC AGGCAGAGAG GGCCCGCGAG GAGGGCATCC GGCTGTTCGC GGTGCCGCCC 
AACCTGAAGC TGAATGAGCA GGGCCTGCGG GACATTGCCA ACACACCCCA CGAGCTCTAC 
CGGAACAACT ACGCGACCAT GCGGCCGGAC TCCACTGAGA TCGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TATGGAGAGT GCTACAAGGT CAGCTGTCTG 
GAGATCCCTG GGCCCCCTGG CCCCAAGGGC TACCGCGGAC AGAAGGGTGC CAAGGGCAAC 
ATGGGTGAGC CTGGAGAGCC TGGACAGAAG GGGCGACAGG GAGATCCAGG CATCGAAGGC 
CCCATTGGAT TCCCTGGACC CAAGGGTGTT CCTGGTTTCA AAGGCGAGAA GGGCGAGTTT 
GGAGCCGACG GGCGGAAGGG TGCCCCGGGC CTGGCGGGCA AGAACGGGAC AGATGGGCAG 
AAGGGCAAGC TGGGGCGCAT TGGACCTCCT GGCTGCAAGG GAGACACCGG GGATCGGGGC 
CCTGATGGCT ACGTGGGGGA AGCCGGCAGC CCTGGGGAGC GAGGAGACCA AGGCTCAAAG 
GGGGACCCTG GCCGCCCAGG ACGCAGAGGC CCCCCCGGGG AGAATGGGGC AAAAGGAAGC 
AAGGGCTATC AAGGCAACAA CGGAGCCCCA GGAAGTCCTG GCCTGAAAGG AGCCAAGGGC 
GGGCCTGGGC CCCGAGGACC CAAAGGCGAG CCTGGGCGCA GGGGAGACCC TGGAACCAAA 
GGCGGCCCAG GCAGTGATGG CCCCAAGGGT GAGAAGGGGG ACCCTGGTCC TGAGGGGCCC 
CGGGGCCTGG CTGGAGAGGT TGGCAACAAA GGAGCCAAGG GAGATCGTGG TTTGCCTGGA 
CCCAGAGGTC CCCAGGGGAC TGTCGGAGAG CCTGGGAAAC AGGGATCTCG GGGAGACCCT 
GGTGATGCTG GTCCCCGCGG AGATTCGGGA CAGCCTGGCC CCAAGGGAGA CCCTGGCAGG 
CCTGGATTCA GCTATCCTGG ACCCCGAGGA GCACCTGGAG ACAAGGGTGA GCCAGGCCCC 
CCCGGCCCCG AGGGGGGCAG AGGTGACTTT GGCTCCAAAG GAGAACCTGG GAGGAAAGGC 
CAGAAGGGAG AGCCTGCAGA TCCTGGTCCC CCTGGTGAGC CTGGCCCACG AGGGCAGAGA 
GGAGCCCCAG GACCTGAGGG AGAGCCTGGC CCCCCAGGAG ACCCCGGCCT CACGGAATGC 
GACGTCATGA CCTACGTGCG GGAGACCTGC GGGTGCTGTG ACTGTGAGAA GCGCTGTGGC 
GCCTTGGATG TGGTGTTCGT CATCGACAGC TCTGAGAGCA TCGGCTACAC CAACTTCACC 
CTGGAGAAGA ACTTCGTCAT CAACGTGGTC AACAGGCTGG GAGCCATCGC CAAGGACCCC 
AAGTCGGAGA CGGGGACCCG CGTGGGCGTC GTGCAGTACA GCCACGAGGG CACCTTCGAA 
GCCATCCAGC TGGACGACGA GCGCATCGAC TCGCTGTCCA GCTTCAAAGA GGCCGTCAAG 
AACCTTGAGT GGATTGCTGG TGGCACCTGG ACGCCCTCGG CCCTCAAGTT CGCCTACAAC 
AAGCTCATCA AGGAGAGCCG GCGCCAGAAG ACCCGCGTGT TCGCGGTGGT CATCACAGAC 
GGCCGCCACG ACCCCCGGGA CGACGACCTC AACCTGCGGG CGCTGTGCAA CCACGAAGTG 
ACGGTGACGG CCATCGGCAT CGGGGACATG TTCCACGAGA AGCACGAGAG CGAGAACCTG 
TACTCCATTG CCTGCGACAA GCCGCAGCAG GTGCGCAACA TGACACTCTT CTCCGACCTG 
GTGGCCGAGA AGTTCATCGA CGACATGGAG GACGTGCTGT GCCCGGATCC CCAGATTGTG 
TGCCCGGATC TTCCCTGCCA AACAGAGCTG TACGTGGCCC AGTGCACACA GCGGCCGGTG 
GACATCGTCT TCTTGCTGGA CGGCTCTGAG CGGCTGGGCG AGCAGAACTT CCACAAGGTG 
CGGCGCTTCG TGGAGGAGGT ATCCCGACGG CTGACCCTGG CGCGCAAGGA CGACGACCCG 
CTCAACGCGC GTGTGGCATT GCTGCAGTTC GGGGGCCCGC GTGAGCAGCA AGTGGCCTTC 
CCGCTGACCT CCAACCTGAC CGTCATTCAG GAGGCGCTGG CGAGCGCGCG CTACCTCAAC 
TCCTTCTCTC ACGTGGGCAC GGGCATCGTG CAGGCCATCA ATCAGGTGGT GCAGGGCGCT 
CGGGCTGGGG CACGCCGCCA CGCGGAGCTG TCCTTCGTGT TCCTCACCGA TGGCGTCACA 
GGCAACGACA GCCTGGATGA GGCGGTACAC TCCATGCGCA AGCAGAACGT GGTGCCCACC 
GTGGTGGCCG TGGGTAGCGA CGTGGACACG GATGTGCTCT CCAAGATCAG CCTGGGCGAC 
CCGGCCGCCG TCTTCCGCGA GAAAGACTAC GACAGCCTGG CCCAGCCCGG CTTCTTCGAC 
AGGTTCATTC GCTGGATCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202110.1
CDS	136..3219
Translation	MLRRKGAAKMLRSPCSALLLWGLLGAVHAQQQEVISPGPSDRNSCPEKADCPVHVYFVLDTSESITMQSPTDSLLYHMQQFVLQFISQLQDELYLDQVALSWRYGGLHFSDLVEVFSPPGSDRASFTKSLQSISSFRRGTFTDCMLANMTQEVRRHVGKGVVNFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVPPNLKLNEQGLRDIANTPHELYRNNYATMRPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPPGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDTGDRGPDGYVGEAGSPGERGDQGSKGDPGRPGRRGPPGENGAKGSKGYQGNNGAPGSPGLKGAKGGPGPRGPKGEPGRRGDPGTKGGPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGTVGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGDKGEPGPPGPEGGRGDFGSKGEPGRKGQKGEPADPGPPGEPGPRGQRGAPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYNKLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCNHEVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELYVAQCTQRPVDIVFLLDGSERLGEQNFHKVRRFVEEVSRRLTLARKDDDPLNARVALLQFGGPREQQVAFPLTSNLTVIQEALASARYLNSFSHVGTGIVQAINQVVQGARAGARRHAELSFVFLTDGVTGNDSLDEAVHSMRKQNVVPTVVAVGSDVDTDVLSKISLGDPAAVFREKDYDSLAQPGFFDRFIRWIC

Target ORF information:

RefSeq Version	XM_005202053.4
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 2 chain (COL6A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202053.4

ORF Insert Sequence:

ATGTTGAGGC GCAAGGGTGC TGCCAAGATG CTCCGCAGCC CCTGCTCTGC CCTCCTGCTC 
TGGGGGCTCC TGGGGGCCGT CCATGCTCAG CAGCAGGAGG TCATCAGCCC TGGCCCCTCG 
GACAGGAATA GCTGCCCTGA GAAGGCCGAC TGCCCGGTCC ACGTGTACTT CGTGCTGGAC 
ACGTCGGAGA GCATCACCAT GCAGTCCCCC ACTGACAGCC TGCTCTACCA CATGCAGCAG 
TTCGTGCTGC AGTTCATCAG CCAACTGCAG GATGAGCTCT ACCTGGACCA GGTGGCCCTG 
AGCTGGCGCT ACGGCGGCCT GCACTTCTCC GATCTGGTAG AGGTGTTCAG CCCGCCGGGC 
AGCGACCGGG CCTCCTTCAC CAAGAGCCTG CAGAGCATCA GCTCCTTCCG CCGGGGCACC 
TTCACCGACT GCATGCTGGC CAACATGACC CAGGAGGTCC GGCGGCATGT GGGCAAGGGC 
GTGGTCAACT TCGCGGTGGT CATCACCGAC GGTCACGTGA CCGGCAGCCC CTGCGGGGGC 
ATCAAGCTGC AGGCAGAGAG GGCCCGCGAG GAGGGCATCC GGCTGTTCGC GGTGCCGCCC 
AACCTGAAGC TGAATGAGCA GGGCCTGCGG GACATTGCCA ACACACCCCA CGAGCTCTAC 
CGGAACAACT ACGCGACCAT GCGGCCGGAC TCCACTGAGA TCGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TATGGAGAGT GCTACAAGGT CAGCTGTCTG 
GAGATCCCTG GGCCCCCTGG CCCCAAGGGC TACCGCGGAC AGAAGGGTGC CAAGGGCAAC 
ATGGGTGAGC CTGGAGAGCC TGGACAGAAG GGGCGACAGG GAGATCCAGG CATCGAAGGC 
CCCATTGGAT TCCCTGGACC CAAGGGTGTT CCTGGTTTCA AAGGCGAGAA GGGCGAGTTT 
GGAGCCGACG GGCGGAAGGG TGCCCCGGGC CTGGCGGGCA AGAACGGGAC AGATGGGCAG 
AAGGGCAAGC TGGGGCGCAT TGGACCTCCT GGCTGCAAGG GAGACACCGG GGATCGGGGC 
CCTGATGGCT ACGTGGGGGA AGCCGGCAGC CCTGGGGAGC GAGGAGACCA AGGCTCAAAG 
GGGGACCCTG GCCGCCCAGG ACGCAGAGGC CCCCCCGGGG AGAATGGGGC AAAAGGAAGC 
AAGGGCTATC AAGGCAACAA CGGAGCCCCA GGAAGTCCTG GCCTGAAAGG AGCCAAGGGC 
GGGCCTGGGC CCCGAGGACC CAAAGGCGAG CCTGGGCGCA GGGGAGACCC TGGAACCAAA 
GGCGGCCCAG GCAGTGATGG CCCCAAGGGT GAGAAGGGGG ACCCTGGTCC TGAGGGGCCC 
CGGGGCCTGG CTGGAGAGGT TGGCAACAAA GGAGCCAAGG GAGATCGTGG TTTGCCTGGA 
CCCAGAGGTC CCCAGGGGAC TGTCGGAGAG CCTGGGAAAC AGGGATCTCG GGGAGACCCT 
GGTGATGCTG GTCCCCGCGG AGATTCGGGA CAGCCTGGCC CCAAGGGAGA CCCTGGCAGG 
CCTGGATTCA GCTATCCTGG ACCCCGAGGA GCACCTGGAG ACAAGGGTGA GCCAGGCCCC 
CCCGGCCCCG AGGGGGGCAG AGGTGACTTT GGCTCCAAAG GAGAACCTGG GAGGAAAGGC 
CAGAAGGGAG AGCCTGCAGA TCCTGGTCCC CCTGGTGAGC CTGGCCCACG AGGGCAGAGA 
GGAGCCCCAG GACCTGAGGG AGAGCCTGGC CCCCCAGGAG ACCCCGGCCT CACGGAATGC 
GACGTCATGA CCTACGTGCG GGAGACCTGC GGGTGCTGTG ACTGTGAGAA GCGCTGTGGC 
GCCTTGGATG TGGTGTTCGT CATCGACAGC TCTGAGAGCA TCGGCTACAC CAACTTCACC 
CTGGAGAAGA ACTTCGTCAT CAACGTGGTC AACAGGCTGG GAGCCATCGC CAAGGACCCC 
AAGTCGGAGA CGGGGACCCG CGTGGGCGTC GTGCAGTACA GCCACGAGGG CACCTTCGAA 
GCCATCCAGC TGGACGACGA GCGCATCGAC TCGCTGTCCA GCTTCAAAGA GGCCGTCAAG 
AACCTTGAGT GGATTGCTGG TGGCACCTGG ACGCCCTCGG CCCTCAAGTT CGCCTACAAC 
AAGCTCATCA AGGAGAGCCG GCGCCAGAAG ACCCGCGTGT TCGCGGTGGT CATCACAGAC 
GGCCGCCACG ACCCCCGGGA CGACGACCTC AACCTGCGGG CGCTGTGCAA CCACGAAGTG 
ACGGTGACGG CCATCGGCAT CGGGGACATG TTCCACGAGA AGCACGAGAG CGAGAACCTG 
TACTCCATTG CCTGCGACAA GCCGCAGCAG GTGCGCAACA TGACACTCTT CTCCGACCTG 
GTGGCCGAGA AGTTCATCGA CGACATGGAG GACGTGCTGT GCCCGGATCC CCAGATTGTG 
TGCCCGGATC TTCCCTGCCA AACAGAGCTG TACGTGGCCC AGTGCACACA GCGGCCGGTG 
GACATCGTCT TCTTGCTGGA CGGCTCTGAG CGGCTGGGCG AGCAGAACTT CCACAAGGTG 
CGGCGCTTCG TGGAGGAGGT ATCCCGACGG CTGACCCTGG CGCGCAAGGA CGACGACCCG 
CTCAACGCGC GTGTGGCATT GCTGCAGTTC GGGGGCCCGC GTGAGCAGCA AGTGGCCTTC 
CCGCTGACCT CCAACCTGAC CGTCATTCAG GAGGCGCTGG CGAGCGCGCG CTACCTCAAC 
TCCTTCTCTC ACGTGGGCAC GGGCATCGTG CAGGCCATCA ATCAGGTGGT GCAGGGCGCT 
CGGGCTGGGG CACGCCGCCA CGCGGAGCTG TCCTTCGTGT TCCTCACCGA TGGCGTCACA 
GGCAACGACA GCCTGGATGA GGCGGTACAC TCCATGCGCA AGCAGAACGT GGTGCCCACC 
GTGGTGGCCG TGGGTAGCGA CGTGGACACG GATGTGCTCT CCAAGATCAG CCTGGGCGAC 
CCGGCCGCCG TCTTCCGCGA GAAAGACTAC GACAGCCTGG CCCAGCCCGG CTTCTTCGAC 
AGGTTCATTC GCTGGATCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( NM_001075126.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001068594.1 Bos taurus collagen type VI alpha 2 chain (COL6A2), mRNA.

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CloneID	OBa38197
Clone ID Related Accession (Same CDS sequence)	NM_001075126.1
Accession Version	NM_001075126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	collagen alpha-2(VI) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC116097.1. On or before Oct 4, 2006 this sequence version replaced XM_874616.1, XM_617127.2. ##Evidence-Data-START## Transcript exon combination :: BC116097.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGCTCCGCA GCCCCTGCTC TGCCCTCCTG CTCTGGGGGC TCCTGGGGGC CGTCCATGCT 
CAGCAGCAGG AGGTCATCAG CCCTGGCCCC TCGGACAGGA ATAGCTGCCC TGAGAAGGCC 
GACTGCCCGG TCCACGTGTA CTTCGTGCTG GACACGTCGG AGAGCATCAC CATGCAGTCC 
CCCACTGACA GCCTGCTCTA CCACATGCAG CAGTTCGTGC TGCAGTTCAT CAGCCAACTG 
CAGGATGAGC TCTACCTGGA CCAGGTGGCC CTGAGCTGGC GCTACGGCGG CCTGCACTTC 
TCCGATCTGG TAGAGGTGTT CAGCCCGCCA GGCAGCGACC GGGCCTCCTT CACCAAGAGC 
CTGCAGAGCA TCAGCTCCTT CCGCCGGGGC ACCTTCACCG ACTGCATGCT GGCCAACATG 
ACCCAGGAGG TCCGGCGGCA TGTGGGCAAG GGCGTGGTCA ACTTCGCGGT GGTCATCACC 
GACGGTCACG TGACCGGCAG CCCCTGCGGG GGCATCAAGC TGCAGGCAGA GAGGGCCCGC 
GAGGAGGGCA TCCGGCTGTT CGCGGTGCCG CCCAACCTGA AGCTGAATGA GCAGGGCCTG 
CGGGACATTG CCAACACACC CCACGAGCTC TACCGGAACA ACTACGCGAC CATGCGGCCG 
GACTCCACTG AGATCGACCA GGACACCATC AACCGCATCA TCAAGGTCAT GAAACATGAA 
GCCTATGGAG AGTGCTACAA GGTCAGCTGT CTGGAGATCC CTGGGCCCCC TGGCCCCAAG 
GGCTACCGCG GACAGAAGGG CGCCAAGGGC AACATGGGTG AGCCTGGAGA GCCTGGACAG 
AAGGGGCGAC AGGGAGATCC AGGCATCGAA GGCCCCATTG GATTCCCTGG ACCCAAGGGT 
GTTCCTGGTT TCAAAGGCGA GAAGGGCGAG TTTGGAGCCG ACGGGCGGAA GGGTGCCCCG 
GGCCTGGCGG GCAAGAACGG GACAGATGGG CAGAAGGGCA AGCTGGGGCG CATTGGACCT 
CCTGGCTGCA AGGGAGACAC CGGGGATCGG GGCCCTGATG GCTACGTGGG GGAAGCCGGC 
AGCCCTGGGG AGCGAGGAGA CCAAGGCTCA AAGGGGGACC CTGGCCGCCC AGGACGCAGA 
GGCCCCCCCG GGGAGAATGG GGCAAAAGGA AGCAAGGGCT ATCAAGGCAA CAACGGAGCC 
CCAGGAAGTC CTGGCCTGAA AGGAGCCAAG GGCGGGCCTG GGCCCCGAGG ACCCAAAGGC 
GAGCCTGGGC GCAGGGGAGA CCCTGGAACC AAAGGCGGCC CAGGCAGTGA TGGCCCCAAG 
GGTGAGAAGG GGGACCCTGG TCCTGAGGGG CCCCGGGGCC TGGCTGGAGA GGTTGGCAAC 
AAAGGAGCCA AGGGAGATCG TGGTTTGCCT GGACCCAGAG GTCCCCAGGG GACTGTCGGA 
GAGCCTGGGA AACAGGGATC TCGGGGAGAC CCTGGTGATG CTGGTCCCCG CGGAGATTCG 
GGACAGCCTG GCCCCAAGGG AGACCCTGGC AGGCCTGGAT TCAGCTATCC TGGACCCCGA 
GGAGCACCTG GAGACAAGGG TGAGCCAGGC CCCCCCGGCC CCGAGGGGGG CAGAGGTGAC 
TTTGGCTCCA AAGGAGAACC TGGGAGGAAA GGCCAGAAGG GAGAGCCTGC AGATCCTGGT 
CCCCCTGGTG AGCCTGGCCC ACGAGGGCAG AGAGGAGCCC CAGGACCTGA GGGAGAGCCT 
GGCCCCCCAG GAGACCCCGG CCTCACGGAA TGCGACGTCA TGACCTACGT GCGGGAGACC 
TGCGGGTGCT GTGACTGTGA GAAGCGCTGT GGCGCCTTGG ATGTGGTGTT CGTCATCGAC 
AGCTCTGAGA GCATCGGCTA CACCAACTTC ACCCTGGAGA AGAACTTCGT CATCAACGTG 
GTCAACAGGC TGGGAGCCAT CGCCAAGGAC CCCAAGTCGG AGACGGGGAC CCGCGTGGGC 
GTCGTGCAGT ACAGCCACGA GGGCACCTTC GAAGCCATCC AGCTGGACGA CGAGCGCATC 
GACTCGCTGT CCAGCTTCAA AGAGGCCGTC AAGAACCTTG AGTGGATTGC TGGTGGCACC 
TGGACGCCCT CGGCCCTCAA GTTCGCCTAC AACAAGCTCA TCAAGGAGAG CCGGCGCCAG 
AAGACCCGCG TGTTCGCGGT GGTCATCACA GACGGCCGCC ACGACCCCCG GGACGACGAC 
CTCAACCTGC GGGCGCTGTG CAACCACGAA GTGACGGTGA CGGCCATCGG CATCGGGGAC 
ATGTTCCACG AGAAGCACGA GAGCGAGAAC CTGTACTCCA TTGCCTGCGA CAAGCCGCAG 
CAGGTGCGCA ACATGACACT CTTCTCCGAC CTGGTGGCCG AGAAGTTCAT CGACGACATG 
GAGGACGTGC TGTGCCCGGA TCCCCAGATT GTGTGCCCGG ATCTTCCCTG CCAAACAGAT 
GGACCGTGGC CTGGCGGCGA GCCCCCGGTC ACCTTCCTCC GCACGGAAGA GGGCCCGGAT 
GTCTCCTTCC CCAGGACCAT CCCCCTGATC CAACAGTTGC TAAACGCCAC GGAGCTCACA 
CAGGACCCAG CTGCCTACTC CAAGCTGGTG GCTGTGCTGG TCTACACTGC GGAGCGGGCC 
AAGTTCGCCA CAGGAAACGA GCGGCAGGAC TGGATGGACC TGTTCATTGA CACCTTTAAA 
CTGGTGCACA GGGACATCGT GGGGGACCCT GAGACCGCGC TGGCGCTGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001068594.1
CDS	45..2798
Translation	MLRSPCSALLLWGLLGAVHAQQQEVISPGPSDRNSCPEKADCPVHVYFVLDTSESITMQSPTDSLLYHMQQFVLQFISQLQDELYLDQVALSWRYGGLHFSDLVEVFSPPGSDRASFTKSLQSISSFRRGTFTDCMLANMTQEVRRHVGKGVVNFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVPPNLKLNEQGLRDIANTPHELYRNNYATMRPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPPGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDTGDRGPDGYVGEAGSPGERGDQGSKGDPGRPGRRGPPGENGAKGSKGYQGNNGAPGSPGLKGAKGGPGPRGPKGEPGRRGDPGTKGGPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGTVGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGDKGEPGPPGPEGGRGDFGSKGEPGRKGQKGEPADPGPPGEPGPRGQRGAPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYNKLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCNHEVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTDGPWPGGEPPVTFLRTEEGPDVSFPRTIPLIQQLLNATELTQDPAAYSKLVAVLVYTAERAKFATGNERQDWMDLFIDTFKLVHRDIVGDPETALALC

Target ORF information:

RefSeq Version	NM_001075126.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 2 chain (COL6A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001075126.1

ORF Insert Sequence:

ATGCTCCGCA GCCCCTGCTC TGCCCTCCTG CTCTGGGGGC TCCTGGGGGC CGTCCATGCT 
CAGCAGCAGG AGGTCATCAG CCCTGGCCCC TCGGACAGGA ATAGCTGCCC TGAGAAGGCC 
GACTGCCCGG TCCACGTGTA CTTCGTGCTG GACACGTCGG AGAGCATCAC CATGCAGTCC 
CCCACTGACA GCCTGCTCTA CCACATGCAG CAGTTCGTGC TGCAGTTCAT CAGCCAACTG 
CAGGATGAGC TCTACCTGGA CCAGGTGGCC CTGAGCTGGC GCTACGGCGG CCTGCACTTC 
TCCGATCTGG TAGAGGTGTT CAGCCCGCCA GGCAGCGACC GGGCCTCCTT CACCAAGAGC 
CTGCAGAGCA TCAGCTCCTT CCGCCGGGGC ACCTTCACCG ACTGCATGCT GGCCAACATG 
ACCCAGGAGG TCCGGCGGCA TGTGGGCAAG GGCGTGGTCA ACTTCGCGGT GGTCATCACC 
GACGGTCACG TGACCGGCAG CCCCTGCGGG GGCATCAAGC TGCAGGCAGA GAGGGCCCGC 
GAGGAGGGCA TCCGGCTGTT CGCGGTGCCG CCCAACCTGA AGCTGAATGA GCAGGGCCTG 
CGGGACATTG CCAACACACC CCACGAGCTC TACCGGAACA ACTACGCGAC CATGCGGCCG 
GACTCCACTG AGATCGACCA GGACACCATC AACCGCATCA TCAAGGTCAT GAAACATGAA 
GCCTATGGAG AGTGCTACAA GGTCAGCTGT CTGGAGATCC CTGGGCCCCC TGGCCCCAAG 
GGCTACCGCG GACAGAAGGG CGCCAAGGGC AACATGGGTG AGCCTGGAGA GCCTGGACAG 
AAGGGGCGAC AGGGAGATCC AGGCATCGAA GGCCCCATTG GATTCCCTGG ACCCAAGGGT 
GTTCCTGGTT TCAAAGGCGA GAAGGGCGAG TTTGGAGCCG ACGGGCGGAA GGGTGCCCCG 
GGCCTGGCGG GCAAGAACGG GACAGATGGG CAGAAGGGCA AGCTGGGGCG CATTGGACCT 
CCTGGCTGCA AGGGAGACAC CGGGGATCGG GGCCCTGATG GCTACGTGGG GGAAGCCGGC 
AGCCCTGGGG AGCGAGGAGA CCAAGGCTCA AAGGGGGACC CTGGCCGCCC AGGACGCAGA 
GGCCCCCCCG GGGAGAATGG GGCAAAAGGA AGCAAGGGCT ATCAAGGCAA CAACGGAGCC 
CCAGGAAGTC CTGGCCTGAA AGGAGCCAAG GGCGGGCCTG GGCCCCGAGG ACCCAAAGGC 
GAGCCTGGGC GCAGGGGAGA CCCTGGAACC AAAGGCGGCC CAGGCAGTGA TGGCCCCAAG 
GGTGAGAAGG GGGACCCTGG TCCTGAGGGG CCCCGGGGCC TGGCTGGAGA GGTTGGCAAC 
AAAGGAGCCA AGGGAGATCG TGGTTTGCCT GGACCCAGAG GTCCCCAGGG GACTGTCGGA 
GAGCCTGGGA AACAGGGATC TCGGGGAGAC CCTGGTGATG CTGGTCCCCG CGGAGATTCG 
GGACAGCCTG GCCCCAAGGG AGACCCTGGC AGGCCTGGAT TCAGCTATCC TGGACCCCGA 
GGAGCACCTG GAGACAAGGG TGAGCCAGGC CCCCCCGGCC CCGAGGGGGG CAGAGGTGAC 
TTTGGCTCCA AAGGAGAACC TGGGAGGAAA GGCCAGAAGG GAGAGCCTGC AGATCCTGGT 
CCCCCTGGTG AGCCTGGCCC ACGAGGGCAG AGAGGAGCCC CAGGACCTGA GGGAGAGCCT 
GGCCCCCCAG GAGACCCCGG CCTCACGGAA TGCGACGTCA TGACCTACGT GCGGGAGACC 
TGCGGGTGCT GTGACTGTGA GAAGCGCTGT GGCGCCTTGG ATGTGGTGTT CGTCATCGAC 
AGCTCTGAGA GCATCGGCTA CACCAACTTC ACCCTGGAGA AGAACTTCGT CATCAACGTG 
GTCAACAGGC TGGGAGCCAT CGCCAAGGAC CCCAAGTCGG AGACGGGGAC CCGCGTGGGC 
GTCGTGCAGT ACAGCCACGA GGGCACCTTC GAAGCCATCC AGCTGGACGA CGAGCGCATC 
GACTCGCTGT CCAGCTTCAA AGAGGCCGTC AAGAACCTTG AGTGGATTGC TGGTGGCACC 
TGGACGCCCT CGGCCCTCAA GTTCGCCTAC AACAAGCTCA TCAAGGAGAG CCGGCGCCAG 
AAGACCCGCG TGTTCGCGGT GGTCATCACA GACGGCCGCC ACGACCCCCG GGACGACGAC 
CTCAACCTGC GGGCGCTGTG CAACCACGAA GTGACGGTGA CGGCCATCGG CATCGGGGAC 
ATGTTCCACG AGAAGCACGA GAGCGAGAAC CTGTACTCCA TTGCCTGCGA CAAGCCGCAG 
CAGGTGCGCA ACATGACACT CTTCTCCGAC CTGGTGGCCG AGAAGTTCAT CGACGACATG 
GAGGACGTGC TGTGCCCGGA TCCCCAGATT GTGTGCCCGG ATCTTCCCTG CCAAACAGAT 
GGACCGTGGC CTGGCGGCGA GCCCCCGGTC ACCTTCCTCC GCACGGAAGA GGGCCCGGAT 
GTCTCCTTCC CCAGGACCAT CCCCCTGATC CAACAGTTGC TAAACGCCAC GGAGCTCACA 
CAGGACCCAG CTGCCTACTC CAAGCTGGTG GCTGTGCTGG TCTACACTGC GGAGCGGGCC 
AAGTTCGCCA CAGGAAACGA GCGGCAGGAC TGGATGGACC TGTTCATTGA CACCTTTAAA 
CTGGTGCACA GGGACATCGT GGGGGACCCT GAGACCGCGC TGGCGCTGTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( XM_005202053.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202110.1 Bos taurus collagen type VI alpha 2 (COL6A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa16124
Clone ID Related Accession (Same CDS sequence)	XM_005202053.3
Accession Version	XM_005202053.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-2(VI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGGC GCAAGGGTGC TGCCAAGATG CTCCGCAGCC CCTGCTCTGC CCTCCTGCTC 
TGGGGGCTCC TGGGGGCCGT CCATGCTCAG CAGCAGGAGG TCATCAGCCC TGGCCCCTCG 
GACAGGAATA GCTGCCCTGA GAAGGCCGAC TGCCCGGTCC ACGTGTACTT CGTGCTGGAC 
ACGTCGGAGA GCATCACCAT GCAGTCCCCC ACTGACAGCC TGCTCTACCA CATGCAGCAG 
TTCGTGCTGC AGTTCATCAG CCAACTGCAG GATGAGCTCT ACCTGGACCA GGTGGCCCTG 
AGCTGGCGCT ACGGCGGCCT GCACTTCTCC GATCTGGTAG AGGTGTTCAG CCCGCCGGGC 
AGCGACCGGG CCTCCTTCAC CAAGAGCCTG CAGAGCATCA GCTCCTTCCG CCGGGGCACC 
TTCACCGACT GCATGCTGGC CAACATGACC CAGGAGGTCC GGCGGCATGT GGGCAAGGGC 
GTGGTCAACT TCGCGGTGGT CATCACCGAC GGTCACGTGA CCGGCAGCCC CTGCGGGGGC 
ATCAAGCTGC AGGCAGAGCG GGCCCGCGAG GAGGGCATCC GGCTGTTCGC GGTGCCGCCC 
AACCTGAAGC TGAATGAGCA GGGCCTGCGG GACATTGCCA ACACACCCCA CGAGCTCTAC 
CGGAACAACT ACGCGACCAT GCGGCCGGAC TCCACTGAGA TCGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TATGGAGAGT GCTACAAGGT CAGCTGTCTG 
GAGATCCCTG GGCCCCCTGG CCCCAAGGGC TACCGCGGAC AGAAGGGCGC CAAGGGCAAC 
ATGGGTGAGC CTGGAGAGCC TGGACAGAAG GGGCGACAGG GAGATCCAGG CATCGAAGGC 
CCCATTGGAT TCCCTGGACC CAAGGGTGTT CCTGGTTTCA AAGGCGAGAA GGGCGAGTTT 
GGAGCCGACG GGCGGAAGGG TGCCCCGGGC CTGGCGGGCA AGAACGGGAC AGATGGGCAG 
AAGGGCAAGC TGGGGCGCAT TGGACCTCCT GGCTGCAAGG GAGACACCGG GGATCGGGGC 
CCTGATGGCT ACGTGGGGGA AGCCGGCAGC CCTGGGGAGC GAGGAGACCA AGGCTCAAAG 
GGGGACCCTG GCCGCCCAGG ACGCAGAGGC CCCCCCGGGG AGAATGGGGC AAAAGGAAGC 
AAGGGCTATC AAGGCAACAA CGGAGCCCCA GGAAGTCCTG GCCTGAAAGG AGCCAAGGGC 
GGGCCTGGGC CCCGAGGACC CAAAGGCGAG CCTGGGCGCA GGGGAGACCC TGGAACCAAA 
GGCGGCCCAG GCAGTGACGG CCCCAAGGGT GAGAAGGGGG ACCCTGGTCC TGAGGGGCCC 
CGGGGCCTGG CTGGAGAGGT TGGCAACAAA GGAGCCAAGG GAGATCGTGG TTTGCCTGGA 
CCCAGAGGTC CCCAGGGGAC TGTCGGAGAG CCTGGGAAAC AGGGATCTCG GGGAGACCCT 
GGTGATGCTG GTCCCCGCGG AGATTCGGGA CAGCCTGGCC CCAAGGGAGA CCCTGGCAGG 
CCTGGATTCA GCTATCCTGG ACCCCGAGGA GCACCTGGAG ACAAGGGTGA GCCAGGCCCC 
CCCGGCCCCG AGGGGGGCAG AGGTGACTTT GGCTCCAAAG GAGAACCTGG GAGGAAAGGC 
CAGAAGGGAG AGCCTGCAGA TCCTGGTCCC CCTGGTGAGC CTGGCCCACG AGGGCAGAGA 
GGAGCCCCAG GACCTGAGGG AGAGCCTGGC CCCCCAGGAG ACCCCGGCCT CACGGAATGC 
GACGTCATGA CCTACGTGCG GGAGACCTGC GGGTGCTGTG ACTGTGAGAA GCGCTGTGGC 
GCCTTGGATG TGGTGTTCGT CATCGACAGC TCTGAGAGCA TCGGCTACAC CAACTTCACC 
CTGGAGAAGA ACTTCGTCAT CAACGTGGTC AACAGGCTGG GAGCCATCGC CAAGGACCCC 
AAGTCGGAGA CGGGGACCCG CGTGGGCGTC GTGCAGTACA GCCACGAGGG CACCTTCGAA 
GCCATCCAGC TGGACGACGA GCGCATCGAC TCGCTGTCCA GCTTCAAAGA GGCCGTCAAG 
AACCTTGAGT GGATTGCTGG TGGCACCTGG ACGCCCTCGG CCCTCAAGTT CGCCTACAAC 
AAGCTCATCA AGGAGAGCCG GCGCCAGAAG ACCCGCGTGT TCGCGGTGGT CATCACAGAC 
GGCCGCCACG ACCCCCGGGA CGACGACCTC AACCTGCGGG CGCTGTGCAA CCACGAAGTG 
ACGGTGACGG CCATCGGCAT CGGGGACATG TTCCACGAGA AGCACGAGAG CGAGAACCTG 
TACTCCATTG CCTGCGACAA GCCGCAGCAG GTGCGCAACA TGACACTCTT CTCCGACCTG 
GTGGCCGAGA AGTTCATCGA CGACATGGAG GACGTGCTGT GCCCGGATCC CCAGATTGTG 
TGCCCGGATC TTCCCTGCCA AACAGAGCTG TACGTGGCCC AGTGCACACA GCGGCCGGTG 
GACATCGTCT TCTTGCTGGA CGGCTCTGAG CGGCTGGGCG AGCAGAACTT CCACAAGGTG 
CGGCGCTTCG TGGAGGAGGT ATCCCGACGG CTGACCCTGG CGCGCAAGGA CGACGACCCG 
CTCAACGCGC GTGTGGCATT GCTGCAGTTC GGGGGCCCGC GTGAGCAGCA AGTGGCCTTC 
CCGCTGACCT CCAACCTGAC CGTCATTCAG GAGGCGCTGG CGAGCGCGCG CTACCTCAAC 
TCCTTCTCTC ACGTGGGCAC GGGCATCGTG CAGGCCATCA ATCAGGTGGT GCAGGGCGCT 
CGGGCCGGGG CACGCCGCCA CGCGGAGCTG TCCTTCGTGT TCCTCACCGA TGGCGTCACA 
GGCAACGACA GCCTGGATGA GGCGGTACAC TCCATGCGCA AGCAGAACGT GGTGCCCACC 
GTGGTGGCCG TGGGTAGCGA CGTGGACACG GATGTGCTCT CCAAGATCAG CCTGGGCGAC 
CCGGCCGCCG TCTTCCGCGA GAAAGACTAC GACAGCCTGG CCCAGCCCGG CTTCTTCGAC 
AGGTTCATTC GCTGGATCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202110.1
CDS	119..3202
Translation	MLRRKGAAKMLRSPCSALLLWGLLGAVHAQQQEVISPGPSDRNSCPEKADCPVHVYFVLDTSESITMQSPTDSLLYHMQQFVLQFISQLQDELYLDQVALSWRYGGLHFSDLVEVFSPPGSDRASFTKSLQSISSFRRGTFTDCMLANMTQEVRRHVGKGVVNFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVPPNLKLNEQGLRDIANTPHELYRNNYATMRPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPPGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDTGDRGPDGYVGEAGSPGERGDQGSKGDPGRPGRRGPPGENGAKGSKGYQGNNGAPGSPGLKGAKGGPGPRGPKGEPGRRGDPGTKGGPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGTVGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGDKGEPGPPGPEGGRGDFGSKGEPGRKGQKGEPADPGPPGEPGPRGQRGAPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYNKLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCNHEVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTELYVAQCTQRPVDIVFLLDGSERLGEQNFHKVRRFVEEVSRRLTLARKDDDPLNARVALLQFGGPREQQVAFPLTSNLTVIQEALASARYLNSFSHVGTGIVQAINQVVQGARAGARRHAELSFVFLTDGVTGNDSLDEAVHSMRKQNVVPTVVAVGSDVDTDVLSKISLGDPAAVFREKDYDSLAQPGFFDRFIRWIC

Target ORF information:

RefSeq Version	XM_005202053.3
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 2 (COL6A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202053.3

ORF Insert Sequence:

ATGTTGAGGC GCAAGGGTGC TGCCAAGATG CTCCGCAGCC CCTGCTCTGC CCTCCTGCTC 
TGGGGGCTCC TGGGGGCCGT CCATGCTCAG CAGCAGGAGG TCATCAGCCC TGGCCCCTCG 
GACAGGAATA GCTGCCCTGA GAAGGCCGAC TGCCCGGTCC ACGTGTACTT CGTGCTGGAC 
ACGTCGGAGA GCATCACCAT GCAGTCCCCC ACTGACAGCC TGCTCTACCA CATGCAGCAG 
TTCGTGCTGC AGTTCATCAG CCAACTGCAG GATGAGCTCT ACCTGGACCA GGTGGCCCTG 
AGCTGGCGCT ACGGCGGCCT GCACTTCTCC GATCTGGTAG AGGTGTTCAG CCCGCCGGGC 
AGCGACCGGG CCTCCTTCAC CAAGAGCCTG CAGAGCATCA GCTCCTTCCG CCGGGGCACC 
TTCACCGACT GCATGCTGGC CAACATGACC CAGGAGGTCC GGCGGCATGT GGGCAAGGGC 
GTGGTCAACT TCGCGGTGGT CATCACCGAC GGTCACGTGA CCGGCAGCCC CTGCGGGGGC 
ATCAAGCTGC AGGCAGAGCG GGCCCGCGAG GAGGGCATCC GGCTGTTCGC GGTGCCGCCC 
AACCTGAAGC TGAATGAGCA GGGCCTGCGG GACATTGCCA ACACACCCCA CGAGCTCTAC 
CGGAACAACT ACGCGACCAT GCGGCCGGAC TCCACTGAGA TCGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TATGGAGAGT GCTACAAGGT CAGCTGTCTG 
GAGATCCCTG GGCCCCCTGG CCCCAAGGGC TACCGCGGAC AGAAGGGCGC CAAGGGCAAC 
ATGGGTGAGC CTGGAGAGCC TGGACAGAAG GGGCGACAGG GAGATCCAGG CATCGAAGGC 
CCCATTGGAT TCCCTGGACC CAAGGGTGTT CCTGGTTTCA AAGGCGAGAA GGGCGAGTTT 
GGAGCCGACG GGCGGAAGGG TGCCCCGGGC CTGGCGGGCA AGAACGGGAC AGATGGGCAG 
AAGGGCAAGC TGGGGCGCAT TGGACCTCCT GGCTGCAAGG GAGACACCGG GGATCGGGGC 
CCTGATGGCT ACGTGGGGGA AGCCGGCAGC CCTGGGGAGC GAGGAGACCA AGGCTCAAAG 
GGGGACCCTG GCCGCCCAGG ACGCAGAGGC CCCCCCGGGG AGAATGGGGC AAAAGGAAGC 
AAGGGCTATC AAGGCAACAA CGGAGCCCCA GGAAGTCCTG GCCTGAAAGG AGCCAAGGGC 
GGGCCTGGGC CCCGAGGACC CAAAGGCGAG CCTGGGCGCA GGGGAGACCC TGGAACCAAA 
GGCGGCCCAG GCAGTGACGG CCCCAAGGGT GAGAAGGGGG ACCCTGGTCC TGAGGGGCCC 
CGGGGCCTGG CTGGAGAGGT TGGCAACAAA GGAGCCAAGG GAGATCGTGG TTTGCCTGGA 
CCCAGAGGTC CCCAGGGGAC TGTCGGAGAG CCTGGGAAAC AGGGATCTCG GGGAGACCCT 
GGTGATGCTG GTCCCCGCGG AGATTCGGGA CAGCCTGGCC CCAAGGGAGA CCCTGGCAGG 
CCTGGATTCA GCTATCCTGG ACCCCGAGGA GCACCTGGAG ACAAGGGTGA GCCAGGCCCC 
CCCGGCCCCG AGGGGGGCAG AGGTGACTTT GGCTCCAAAG GAGAACCTGG GAGGAAAGGC 
CAGAAGGGAG AGCCTGCAGA TCCTGGTCCC CCTGGTGAGC CTGGCCCACG AGGGCAGAGA 
GGAGCCCCAG GACCTGAGGG AGAGCCTGGC CCCCCAGGAG ACCCCGGCCT CACGGAATGC 
GACGTCATGA CCTACGTGCG GGAGACCTGC GGGTGCTGTG ACTGTGAGAA GCGCTGTGGC 
GCCTTGGATG TGGTGTTCGT CATCGACAGC TCTGAGAGCA TCGGCTACAC CAACTTCACC 
CTGGAGAAGA ACTTCGTCAT CAACGTGGTC AACAGGCTGG GAGCCATCGC CAAGGACCCC 
AAGTCGGAGA CGGGGACCCG CGTGGGCGTC GTGCAGTACA GCCACGAGGG CACCTTCGAA 
GCCATCCAGC TGGACGACGA GCGCATCGAC TCGCTGTCCA GCTTCAAAGA GGCCGTCAAG 
AACCTTGAGT GGATTGCTGG TGGCACCTGG ACGCCCTCGG CCCTCAAGTT CGCCTACAAC 
AAGCTCATCA AGGAGAGCCG GCGCCAGAAG ACCCGCGTGT TCGCGGTGGT CATCACAGAC 
GGCCGCCACG ACCCCCGGGA CGACGACCTC AACCTGCGGG CGCTGTGCAA CCACGAAGTG 
ACGGTGACGG CCATCGGCAT CGGGGACATG TTCCACGAGA AGCACGAGAG CGAGAACCTG 
TACTCCATTG CCTGCGACAA GCCGCAGCAG GTGCGCAACA TGACACTCTT CTCCGACCTG 
GTGGCCGAGA AGTTCATCGA CGACATGGAG GACGTGCTGT GCCCGGATCC CCAGATTGTG 
TGCCCGGATC TTCCCTGCCA AACAGAGCTG TACGTGGCCC AGTGCACACA GCGGCCGGTG 
GACATCGTCT TCTTGCTGGA CGGCTCTGAG CGGCTGGGCG AGCAGAACTT CCACAAGGTG 
CGGCGCTTCG TGGAGGAGGT ATCCCGACGG CTGACCCTGG CGCGCAAGGA CGACGACCCG 
CTCAACGCGC GTGTGGCATT GCTGCAGTTC GGGGGCCCGC GTGAGCAGCA AGTGGCCTTC 
CCGCTGACCT CCAACCTGAC CGTCATTCAG GAGGCGCTGG CGAGCGCGCG CTACCTCAAC 
TCCTTCTCTC ACGTGGGCAC GGGCATCGTG CAGGCCATCA ATCAGGTGGT GCAGGGCGCT 
CGGGCCGGGG CACGCCGCCA CGCGGAGCTG TCCTTCGTGT TCCTCACCGA TGGCGTCACA 
GGCAACGACA GCCTGGATGA GGCGGTACAC TCCATGCGCA AGCAGAACGT GGTGCCCACC 
GTGGTGGCCG TGGGTAGCGA CGTGGACACG GATGTGCTCT CCAAGATCAG CCTGGGCGAC 
CCGGCCGCCG TCTTCCGCGA GAAAGACTAC GACAGCCTGG CCCAGCCCGG CTTCTTCGAC 
AGGTTCATTC GCTGGATCTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL6A2 ( XM_010801673.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010799975.1 Bos taurus collagen type VI alpha 2 (COL6A2), transcript variant X2, mRNA.

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CloneID	OBa45392
Clone ID Related Accession (Same CDS sequence)	XM_010801673.2
Accession Version	XM_010801673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	collagen alpha-2(VI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGGC GCAAGGGTGC TGCCAAGATG CTCCGCAGCC CCTGCTCTGC CCTCCTGCTC 
TGGGGGCTCC TGGGGGCCGT CCATGCTCAG CAGCAGGAGG TCATCAGCCC TGGCCCCTCG 
GACAGGAATA GCTGCCCTGA GAAGGCCGAC TGCCCGGTCC ACGTGTACTT CGTGCTGGAC 
ACGTCGGAGA GCATCACCAT GCAGTCCCCC ACTGACAGCC TGCTCTACCA CATGCAGCAG 
TTCGTGCTGC AGTTCATCAG CCAACTGCAG GATGAGCTCT ACCTGGACCA GGTGGCCCTG 
AGCTGGCGCT ACGGCGGCCT GCACTTCTCC GATCTGGTAG AGGTGTTCAG CCCGCCGGGC 
AGCGACCGGG CCTCCTTCAC CAAGAGCCTG CAGAGCATCA GCTCCTTCCG CCGGGGCACC 
TTCACCGACT GCATGCTGGC CAACATGACC CAGGAGGTCC GGCGGCATGT GGGCAAGGGC 
GTGGTCAACT TCGCGGTGGT CATCACCGAC GGTCACGTGA CCGGCAGCCC CTGCGGGGGC 
ATCAAGCTGC AGGCAGAGCG GGCCCGCGAG GAGGGCATCC GGCTGTTCGC GGTGCCGCCC 
AACCTGAAGC TGAATGAGCA GGGCCTGCGG GACATTGCCA ACACACCCCA CGAGCTCTAC 
CGGAACAACT ACGCGACCAT GCGGCCGGAC TCCACTGAGA TCGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TATGGAGAGT GCTACAAGGT CAGCTGTCTG 
GAGATCCCTG GGCCCCCTGG CCCCAAGGGC TACCGCGGAC AGAAGGGCGC CAAGGGCAAC 
ATGGGTGAGC CTGGAGAGCC TGGACAGAAG GGGCGACAGG GAGATCCAGG CATCGAAGGC 
CCCATTGGAT TCCCTGGACC CAAGGGTGTT CCTGGTTTCA AAGGCGAGAA GGGCGAGTTT 
GGAGCCGACG GGCGGAAGGG TGCCCCGGGC CTGGCGGGCA AGAACGGGAC AGATGGGCAG 
AAGGGCAAGC TGGGGCGCAT TGGACCTCCT GGCTGCAAGG GAGACACCGG GGATCGGGGC 
CCTGATGGCT ACGTGGGGGA AGCCGGCAGC CCTGGGGAGC GAGGAGACCA AGGCTCAAAG 
GGGGACCCTG GCCGCCCAGG ACGCAGAGGC CCCCCCGGGG AGAATGGGGC AAAAGGAAGC 
AAGGGCTATC AAGGCAACAA CGGAGCCCCA GGAAGTCCTG GCCTGAAAGG AGCCAAGGGC 
GGGCCTGGGC CCCGAGGACC CAAAGGCGAG CCTGGGCGCA GGGGAGACCC TGGAACCAAA 
GGCGGCCCAG GCAGTGACGG CCCCAAGGGT GAGAAGGGGG ACCCTGGTCC TGAGGGGCCC 
CGGGGCCTGG CTGGAGAGGT TGGCAACAAA GGAGCCAAGG GAGATCGTGG TTTGCCTGGA 
CCCAGAGGTC CCCAGGGGAC TGTCGGAGAG CCTGGGAAAC AGGGATCTCG GGGAGACCCT 
GGTGATGCTG GTCCCCGCGG AGATTCGGGA CAGCCTGGCC CCAAGGGAGA CCCTGGCAGG 
CCTGGATTCA GCTATCCTGG ACCCCGAGGA GCACCTGGAG ACAAGGGTGA GCCAGGCCCC 
CCCGGCCCCG AGGGGGGCAG AGGTGACTTT GGCTCCAAAG GAGAACCTGG GAGGAAAGGC 
CAGAAGGGAG AGCCTGCAGA TCCTGGTCCC CCTGGTGAGC CTGGCCCACG AGGGCAGAGA 
GGAGCCCCAG GACCTGAGGG AGAGCCTGGC CCCCCAGGAG ACCCCGGCCT CACGGAATGC 
GACGTCATGA CCTACGTGCG GGAGACCTGC GGGTGCTGTG ACTGTGAGAA GCGCTGTGGC 
GCCTTGGATG TGGTGTTCGT CATCGACAGC TCTGAGAGCA TCGGCTACAC CAACTTCACC 
CTGGAGAAGA ACTTCGTCAT CAACGTGGTC AACAGGCTGG GAGCCATCGC CAAGGACCCC 
AAGTCGGAGA CGGGGACCCG CGTGGGCGTC GTGCAGTACA GCCACGAGGG CACCTTCGAA 
GCCATCCAGC TGGACGACGA GCGCATCGAC TCGCTGTCCA GCTTCAAAGA GGCCGTCAAG 
AACCTTGAGT GGATTGCTGG TGGCACCTGG ACGCCCTCGG CCCTCAAGTT CGCCTACAAC 
AAGCTCATCA AGGAGAGCCG GCGCCAGAAG ACCCGCGTGT TCGCGGTGGT CATCACAGAC 
GGCCGCCACG ACCCCCGGGA CGACGACCTC AACCTGCGGG CGCTGTGCAA CCACGAAGTG 
ACGGTGACGG CCATCGGCAT CGGGGACATG TTCCACGAGA AGCACGAGAG CGAGAACCTG 
TACTCCATTG CCTGCGACAA GCCGCAGCAG GTGCGCAACA TGACACTCTT CTCCGACCTG 
GTGGCCGAGA AGTTCATCGA CGACATGGAG GACGTGCTGT GCCCGGATCC CCAGATTGTG 
TGCCCGGATC TTCCCTGCCA AACAGTAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010799975.1
CDS	119..2611
Translation	MLRRKGAAKMLRSPCSALLLWGLLGAVHAQQQEVISPGPSDRNSCPEKADCPVHVYFVLDTSESITMQSPTDSLLYHMQQFVLQFISQLQDELYLDQVALSWRYGGLHFSDLVEVFSPPGSDRASFTKSLQSISSFRRGTFTDCMLANMTQEVRRHVGKGVVNFAVVITDGHVTGSPCGGIKLQAERAREEGIRLFAVPPNLKLNEQGLRDIANTPHELYRNNYATMRPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPPGPKGYRGQKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGADGRKGAPGLAGKNGTDGQKGKLGRIGPPGCKGDTGDRGPDGYVGEAGSPGERGDQGSKGDPGRPGRRGPPGENGAKGSKGYQGNNGAPGSPGLKGAKGGPGPRGPKGEPGRRGDPGTKGGPGSDGPKGEKGDPGPEGPRGLAGEVGNKGAKGDRGLPGPRGPQGTVGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGAPGDKGEPGPPGPEGGRGDFGSKGEPGRKGQKGEPADPGPPGEPGPRGQRGAPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALDVVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIQLDDERIDSLSSFKEAVKNLEWIAGGTWTPSALKFAYNKLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCNHEVTVTAIGIGDMFHEKHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPDLPCQTVN

Target ORF information:

RefSeq Version	XM_010801673.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type VI alpha 2 (COL6A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801673.2

ORF Insert Sequence:

ATGTTGAGGC GCAAGGGTGC TGCCAAGATG CTCCGCAGCC CCTGCTCTGC CCTCCTGCTC 
TGGGGGCTCC TGGGGGCCGT CCATGCTCAG CAGCAGGAGG TCATCAGCCC TGGCCCCTCG 
GACAGGAATA GCTGCCCTGA GAAGGCCGAC TGCCCGGTCC ACGTGTACTT CGTGCTGGAC 
ACGTCGGAGA GCATCACCAT GCAGTCCCCC ACTGACAGCC TGCTCTACCA CATGCAGCAG 
TTCGTGCTGC AGTTCATCAG CCAACTGCAG GATGAGCTCT ACCTGGACCA GGTGGCCCTG 
AGCTGGCGCT ACGGCGGCCT GCACTTCTCC GATCTGGTAG AGGTGTTCAG CCCGCCGGGC 
AGCGACCGGG CCTCCTTCAC CAAGAGCCTG CAGAGCATCA GCTCCTTCCG CCGGGGCACC 
TTCACCGACT GCATGCTGGC CAACATGACC CAGGAGGTCC GGCGGCATGT GGGCAAGGGC 
GTGGTCAACT TCGCGGTGGT CATCACCGAC GGTCACGTGA CCGGCAGCCC CTGCGGGGGC 
ATCAAGCTGC AGGCAGAGCG GGCCCGCGAG GAGGGCATCC GGCTGTTCGC GGTGCCGCCC 
AACCTGAAGC TGAATGAGCA GGGCCTGCGG GACATTGCCA ACACACCCCA CGAGCTCTAC 
CGGAACAACT ACGCGACCAT GCGGCCGGAC TCCACTGAGA TCGACCAGGA CACCATCAAC 
CGCATCATCA AGGTCATGAA ACATGAAGCC TATGGAGAGT GCTACAAGGT CAGCTGTCTG 
GAGATCCCTG GGCCCCCTGG CCCCAAGGGC TACCGCGGAC AGAAGGGCGC CAAGGGCAAC 
ATGGGTGAGC CTGGAGAGCC TGGACAGAAG GGGCGACAGG GAGATCCAGG CATCGAAGGC 
CCCATTGGAT TCCCTGGACC CAAGGGTGTT CCTGGTTTCA AAGGCGAGAA GGGCGAGTTT 
GGAGCCGACG GGCGGAAGGG TGCCCCGGGC CTGGCGGGCA AGAACGGGAC AGATGGGCAG 
AAGGGCAAGC TGGGGCGCAT TGGACCTCCT GGCTGCAAGG GAGACACCGG GGATCGGGGC 
CCTGATGGCT ACGTGGGGGA AGCCGGCAGC CCTGGGGAGC GAGGAGACCA AGGCTCAAAG 
GGGGACCCTG GCCGCCCAGG ACGCAGAGGC CCCCCCGGGG AGAATGGGGC AAAAGGAAGC 
AAGGGCTATC AAGGCAACAA CGGAGCCCCA GGAAGTCCTG GCCTGAAAGG AGCCAAGGGC 
GGGCCTGGGC CCCGAGGACC CAAAGGCGAG CCTGGGCGCA GGGGAGACCC TGGAACCAAA 
GGCGGCCCAG GCAGTGACGG CCCCAAGGGT GAGAAGGGGG ACCCTGGTCC TGAGGGGCCC 
CGGGGCCTGG CTGGAGAGGT TGGCAACAAA GGAGCCAAGG GAGATCGTGG TTTGCCTGGA 
CCCAGAGGTC CCCAGGGGAC TGTCGGAGAG CCTGGGAAAC AGGGATCTCG GGGAGACCCT 
GGTGATGCTG GTCCCCGCGG AGATTCGGGA CAGCCTGGCC CCAAGGGAGA CCCTGGCAGG 
CCTGGATTCA GCTATCCTGG ACCCCGAGGA GCACCTGGAG ACAAGGGTGA GCCAGGCCCC 
CCCGGCCCCG AGGGGGGCAG AGGTGACTTT GGCTCCAAAG GAGAACCTGG GAGGAAAGGC 
CAGAAGGGAG AGCCTGCAGA TCCTGGTCCC CCTGGTGAGC CTGGCCCACG AGGGCAGAGA 
GGAGCCCCAG GACCTGAGGG AGAGCCTGGC CCCCCAGGAG ACCCCGGCCT CACGGAATGC 
GACGTCATGA CCTACGTGCG GGAGACCTGC GGGTGCTGTG ACTGTGAGAA GCGCTGTGGC 
GCCTTGGATG TGGTGTTCGT CATCGACAGC TCTGAGAGCA TCGGCTACAC CAACTTCACC 
CTGGAGAAGA ACTTCGTCAT CAACGTGGTC AACAGGCTGG GAGCCATCGC CAAGGACCCC 
AAGTCGGAGA CGGGGACCCG CGTGGGCGTC GTGCAGTACA GCCACGAGGG CACCTTCGAA 
GCCATCCAGC TGGACGACGA GCGCATCGAC TCGCTGTCCA GCTTCAAAGA GGCCGTCAAG 
AACCTTGAGT GGATTGCTGG TGGCACCTGG ACGCCCTCGG CCCTCAAGTT CGCCTACAAC 
AAGCTCATCA AGGAGAGCCG GCGCCAGAAG ACCCGCGTGT TCGCGGTGGT CATCACAGAC 
GGCCGCCACG ACCCCCGGGA CGACGACCTC AACCTGCGGG CGCTGTGCAA CCACGAAGTG 
ACGGTGACGG CCATCGGCAT CGGGGACATG TTCCACGAGA AGCACGAGAG CGAGAACCTG 
TACTCCATTG CCTGCGACAA GCCGCAGCAG GTGCGCAACA TGACACTCTT CTCCGACCTG 
GTGGCCGAGA AGTTCATCGA CGACATGGAG GACGTGCTGT GCCCGGATCC CCAGATTGTG 
TGCCCGGATC TTCCCTGCCA AACAGTAAAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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COL6A2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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