TIMP2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMP2
Entrez Gene ID	282093
Full Name	TIMP metallopeptidase inhibitor 2
General protein information	Preferred Names TIMP metallopeptidase inhibitor 2 Names metalloproteinase inhibitor 2 TIMP-2 collagenase inhibitor tissue inhibitor of metalloproteinases 2 tissue inhibitor of mettaloproteinase 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174472.4	NP_776897.2	metalloproteinase inhibitor 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIMP2	NP_003246.1
Bos taurus (cattle)	TIMP2	NP_776897.2
Pan troglodytes (chimpanzee)	TIMP2	XP_001146829.2
	TIMP2	XP_001106657.2
Gallus gallus (chicken)	TIMP2	NP_989629.1
Danio rerio (zebrafish)	timp2a	NP_878294.1
Xenopus tropicalis (tropical clawed frog)	timp2	NP_001015760.1
Canis lupus familiaris (dog)	TIMP2	NP_001003082.1
Mus musculus (house mouse)	Timp2	NP_035724.2
Rattus norvegicus (Norway rat)	Timp2	NP_068824.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of PKC-? in NF-?B-MT1-MMP-mediated activation of proMMP-2 by TNF-? in pulmonary artery smooth muscle cells.
Roy S, Chakraborti T, Chowdhury A, Chakraborti S
Journal of biochemistry153(3)289-302(2013 Mar)

Differential immunohistochemical expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in cow uteri with adenomyosis during follicular phase.
Moreira L, de Carvalho EC, Caldas-Bussiere MC
Veterinary research communications35(5)261-9(2011 Jun)

Expression of matrix metalloproteinase (MMP)-2, MMP-14 and tissue inhibitor of matrix metalloproteinase (TIMP)-2 during bovine placentation and at term with or without placental retention.
Dilly M, Hambruch N, Shenavai S, Schuler G, Froehlich R, Haeger JD, Ozalp GR, Pfarrer C
Theriogenology75(6)1104-14(2011 Apr)

Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy.
Ulbrich SE, Meyer SU, Zitta K, Hiendleder S, Sinowatz F, Bauersachs S, B?ttner M, Fr?hlich T, Arnold GJ, Reichenbach HD, Wolf E, Meyer HH
Molecular and cellular endocrinology332(1-2)48-57(2011 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate the involvement of PKC-alpha in proMMP-2 activation and inhibition of TIMP-2 expression by NF-kappaB-MT1-MMP-dependent and -independent pathway.
Title: Role of PKC-? in NF-?B-MT1-MMP-mediated activation of proMMP-2 by TNF-? in pulmonary artery smooth muscle cells.

A differential pattern of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 was observed in cow uteri with adenomyosis.
Title: Differential immunohistochemical expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in cow uteri with adenomyosis during follicular phase.

MMP-14, MMP-2 and TIMP-2 are co-localized in the fetal compartment and therefore could influence the timely release of fetal membranes in cattle.
Title: Expression of matrix metalloproteinase (MMP)-2, MMP-14 and tissue inhibitor of matrix metalloproteinase (TIMP)-2 during bovine placentation and at term with or without placental retention.

Results describe distinct changes in expression of MMP2, MMP14, and the metallopeptidase inhibitor TIMP2 between different phases of the estrous cycle indicating an endocrine regulation.
Title: Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy.

legumain and TIMP-2 mRNAs were up-regulated in the endometrium during the luteal phase of the oestrous cycle and during early pregnancy
Title: Bovine endometrial legumain and TIMP-2 regulation in response to presence of a conceptus.

The following TIMP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa00456	NM_174472.4 Latest version!	Bos taurus TIMP metallopeptidase inhibitor 2 (TIMP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMP2 ( NM_174472.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776897.2 Bos taurus TIMP metallopeptidase inhibitor 2 (TIMP2), mRNA.

Price & Availability↑

CloneID	OBa00456
Clone ID Related Accession (Same CDS sequence)	NM_174472.4
Accession Version	NM_174472.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Bos taurus(cattle)
Product	metalloproteinase inhibitor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102710.1. On Aug 31, 2012 this sequence version replaced NM_174472.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC102710.1, M32303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGGCGCCG CGGCCCGCAG CCTGCCGCTC GCGTTCTGCC TCATGCTGCT GGGGACGCTG 
CTCCCCCGGG CCGACGCCTG CAGCTGCTCC CCGGTGCACC CGCAACAGGC GTTTTGCAAT 
GCAGACATAG TGATCAGGGC CAAAGCAGTC AATAAGAAGG AGGTGGACTC TGGCAACGAC 
ATCTACGGCA ACCCCATCAA GCGGATTCAG TATGAGATCA AGCAGATAAA GATGTTCAAG 
GGACCTGATC AGGACATAGA GTTGATCTAC ACAGCCCCCT CCTCTGCCGT GTGTGGGGTC 
TCGCTGGACA TTGGAGGAAA GAAGGAGTAT CTCATTGCAG GGAAGGCCGA GGGGAATGGC 
AATATGCATA TCACCCTCTG TGACTTCATC GTGCCCTGGG ACACCCTGAG TGCCACCCAG 
AAGAAGAGCC TGAACCACAG GTACCAGATG GGCTGTGAGT GCAAGATCAC TCGATGCCCC 
ATGATCCCAT GCTACATCTC CTCTCCGGAC GAGTGCCTCT GGATGGACTG GGTCACGGAG 
AAGAACATCA ACGGACACCA GGCCAAGTTC TTCGCCTGCA TCAAGAGAAG CGACGGCTCC 
TGCGCCTGGT ACCGCGGAGC AGCACCCCCC AAGCAGGAGT TTCTGGACAT CGAGGACCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776897.2
CDS	302..964
Translation	MGAAARSLPLAFCLMLLGTLLPRADACSCSPVHPQQAFCNADIVIRAKAVNKKEVDSGNDIYGNPIKRIQYEIKQIKMFKGPDQDIELIYTAPSSAVCGVSLDIGGKKEYLIAGKAEGNGNMHITLCDFIVPWDTLSATQKKSLNHRYQMGCECKITRCPMIPCYISSPDECLWMDWVTEKNINGHQAKFFACIKRSDGSCAWYRGAAPPKQEFLDIEDP

Target ORF information:

RefSeq Version	NM_174472.4
Organism	Bos taurus(cattle)
Definition	Bos taurus TIMP metallopeptidase inhibitor 2 (TIMP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174472.4

ORF Insert Sequence:

ATGGGCGCCG CGGCCCGCAG CCTGCCGCTC GCGTTCTGCC TCATGCTGCT GGGGACGCTG 
CTCCCCCGGG CCGACGCCTG CAGCTGCTCC CCGGTGCACC CGCAACAGGC GTTTTGCAAT 
GCAGACATAG TGATCAGGGC CAAAGCAGTC AATAAGAAGG AGGTGGACTC TGGCAACGAC 
ATCTACGGCA ACCCCATCAA GCGGATTCAG TATGAGATCA AGCAGATAAA GATGTTCAAG 
GGACCTGATC AGGACATAGA GTTGATCTAC ACAGCCCCCT CCTCTGCCGT GTGTGGGGTC 
TCGCTGGACA TTGGAGGAAA GAAGGAGTAT CTCATTGCAG GGAAGGCCGA GGGGAATGGC 
AATATGCATA TCACCCTCTG TGACTTCATC GTGCCCTGGG ACACCCTGAG TGCCACCCAG 
AAGAAGAGCC TGAACCACAG GTACCAGATG GGCTGTGAGT GCAAGATCAC TCGATGCCCC 
ATGATCCCAT GCTACATCTC CTCTCCGGAC GAGTGCCTCT GGATGGACTG GGTCACGGAG 
AAGAACATCA ACGGACACCA GGCCAAGTTC TTCGCCTGCA TCAAGAGAAG CGACGGCTCC 
TGCGCCTGGT ACCGCGGAGC AGCACCCCCC AAGCAGGAGT TTCTGGACAT CGAGGACCCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of PKC-? in NF-?B-MT1-MMP-mediated activation of proMMP-2 by TNF-? in pulmonary artery smooth muscle cells.
Journal of biochemistry153(3)289-302(2013 Mar)
Roy S,Chakraborti T,Chowdhury A,Chakraborti S

Differential immunohistochemical expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in cow uteri with adenomyosis during follicular phase.
Veterinary research communications35(5)261-9(2011 Jun)
Moreira L,de Carvalho EC,Caldas-Bussiere MC

Expression of matrix metalloproteinase (MMP)-2, MMP-14 and tissue inhibitor of matrix metalloproteinase (TIMP)-2 during bovine placentation and at term with or without placental retention.
Theriogenology75(6)1104-14(2011 Apr)
Dilly M,Hambruch N,Shenavai S,Schuler G,Froehlich R,Haeger JD,Ozalp GR,Pfarrer C

Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy.
Molecular and cellular endocrinology332(1-2)48-57(2011 Jan)
Ulbrich SE,Meyer SU,Zitta K,Hiendleder S,Sinowatz F,Bauersachs S,B?ttner M,Fr?hlich T,Arnold GJ,Reichenbach HD,Wolf E,Meyer HH

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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TIMP2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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