SYNJ1 cDNA ORF clone, Bos taurus(cattle)

The following SYNJ1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SYNJ1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa218603 XM_024992101.1
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa218604 XM_024992193.1
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa87628 XM_015464030.1
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa44412 NM_174468.3
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa45338 XM_005201097.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa218601 XM_024991867.1
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218602 XM_024991927.1
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218602 XM_024991959.1
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa218602 XM_024991891.1
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa87627 XM_015463989.1
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa45325 XM_005201086.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45326 XM_005201087.3
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45327 XM_005201088.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45328 XM_005201089.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45329 XM_005201090.3
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45330 XM_010800891.3
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45331 XM_010800892.2
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45332 XM_005201091.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45333 XM_005201092.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45334 XM_005201093.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45335 XM_005201094.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45336 XM_005201095.4
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45337 XM_005201096.3
Latest version!
Bos taurus synaptojanin 1 (SYNJ1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa45327 XM_005201088.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45330 XM_010800891.2 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45334 XM_005201093.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45325 XM_005201086.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45328 XM_005201089.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45335 XM_005201094.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45336 XM_005201095.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45333 XM_005201092.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45338 XM_005201097.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa45332 XM_005201091.3 Bos taurus synaptojanin 1 (SYNJ1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa218603
    Clone ID Related Accession (Same CDS sequence) XM_024992101.1
    Accession Version XM_024992101.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGA CGCAGCCAGC TTCCACCTCA AGCCGGACTG
    CCAGGCCCGG GACTTGCTGG ACACAGTGCA GCCAGACCGA TTATTCCGCC CCGTGCTGGA
    GTCATCAGCG CCCCCCAGAG CCACGCACGG GTGTCTGCTG GGAGACTGAC TCCTGAAAGC
    CAAAGCAAAA CCTCAGAAGT ACTGAAAGGG CCAGCTCTTC TTCCCGAGCC CCTGAAGCCT
    CAGGCCGCCC TTCCTGTGCC GCCTTCTCTT GCGCCACCTT CTCTTGCGCC ACCTGTTCAG
    AAGATGCAGG AGCCTCTCAT CCCGGTGGCC GCACCTCTGG CCCAGGCTGC CCTGCAGCCC
    AGCCTGGAAA CGCCCCCGCA GCCACCCCCT CGGAGCAGGT CGTCCCACAG CTTGCCTTCT
    GAGCCTCCGG CGCAGCCGCA GGAGCAACCA TCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847869.1
    CDS16..3954
    Translation

    Target ORF information:

    RefSeq Version XM_024992101.1
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992101.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGA CGCAGCCAGC TTCCACCTCA AGCCGGACTG
    CCAGGCCCGG GACTTGCTGG ACACAGTGCA GCCAGACCGA TTATTCCGCC CCGTGCTGGA
    GTCATCAGCG CCCCCCAGAG CCACGCACGG GTGTCTGCTG GGAGACTGAC TCCTGAAAGC
    CAAAGCAAAA CCTCAGAAGT ACTGAAAGGG CCAGCTCTTC TTCCCGAGCC CCTGAAGCCT
    CAGGCCGCCC TTCCTGTGCC GCCTTCTCTT GCGCCACCTT CTCTTGCGCC ACCTGTTCAG
    AAGATGCAGG AGCCTCTCAT CCCGGTGGCC GCACCTCTGG CCCAGGCTGC CCTGCAGCCC
    AGCCTGGAAA CGCCCCCGCA GCCACCCCCT CGGAGCAGGT CGTCCCACAG CTTGCCTTCT
    GAGCCTCCGG CGCAGCCGCA GGAGCAACCA TCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218604
    Clone ID Related Accession (Same CDS sequence) XM_024992193.1
    Accession Version XM_024992193.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGACG CAGCCAGCTT CCACCTCAAG CCGGACTGCC AGGCCCGGGA
    CTTGCTGGAC ACAGTGCAGC CAGACCGATT ATTCCGCCCC GTGCTGGAGT CATCAGCGCC
    CCCCAGAGCC ACGCACGGGT GTCTGCTGGG AGACTGACTC CTGAAAGCCA AAGCAAAACC
    TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT
    CCTGTGCCGC CTTCTCTTGC GCCACCTTCT CTTGCGCCAC CTGTTCAGAA GATGCAGGAG
    CCTCTCATCC CGGTGGCCGC ACCTCTGGCC CAGGCTGCCC TGCAGCCCAG CCTGGAAACG
    CCCCCGCAGC CACCCCCTCG GAGCAGGTCG TCCCACAGCT TGCCTTCTGA GCCTCCGGCG
    CAGCCGCAGG AGCAACCATC AGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847961.1
    CDS94..3900
    Translation

    Target ORF information:

    RefSeq Version XM_024992193.1
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGACG CAGCCAGCTT CCACCTCAAG CCGGACTGCC AGGCCCGGGA
    CTTGCTGGAC ACAGTGCAGC CAGACCGATT ATTCCGCCCC GTGCTGGAGT CATCAGCGCC
    CCCCAGAGCC ACGCACGGGT GTCTGCTGGG AGACTGACTC CTGAAAGCCA AAGCAAAACC
    TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT
    CCTGTGCCGC CTTCTCTTGC GCCACCTTCT CTTGCGCCAC CTGTTCAGAA GATGCAGGAG
    CCTCTCATCC CGGTGGCCGC ACCTCTGGCC CAGGCTGCCC TGCAGCCCAG CCTGGAAACG
    CCCCCGCAGC CACCCCCTCG GAGCAGGTCG TCCCACAGCT TGCCTTCTGA GCCTCCGGCG
    CAGCCGCAGG AGCAACCATC AGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87628
    Clone ID Related Accession (Same CDS sequence) XM_015464030.1
    Accession Version XM_015464030.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GTTAAAAGAT GGTGCTCGTT CAGTTAGTAG AACAATTCAG
    AATAACTTCT TTGACAGCTC CAAGCAAGAA GCAATTGATG TTTTGCTCCT GGGAAATACT
    CTAAATAGTG ATTTAGCTGA CAAAGCTCGA GCCCTTTTAA CTACTGGAAG TTTGCGTGTT
    TCTGAACAGA CGTTACAGTC AGCATCTTCT AAAGTACTAA AGAGTATGTG TGAGAATTTC
    TACAAGTATT CAAAGCCCAA GAAAATTCGA GTATGTGTGG GCACATGGAA CGTGAATGGC
    GGGAAGCAGT TTCGCAGCAT AGCTTTTAAG AATCAGACCC TTACGGACTG GCTTCTTGAT
    GCCCCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA GAAGTAAACC AATGGATATA
    TTTGCAATTG GTTTTGAGGA AATGGTAGAG CTGAATGCTG GAAACATTGT GAATGCAAGC
    ACAACAAATC AGAAGCTCTG GGCTGCGGAA CTTCAGAAGA CCATCTCCAG AGACAACAAG
    TATGTGCTGC TGGCCTCTGA GCAGTTGGTG GGCGTCTGTC TGTTTGTTTT TATCAGACCA
    CAGCACGCTC CCTTCATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG AATGGGAGGC
    GCAACTGGAA ATAAGGGAGC AGTTGCAATA CGAATGCTGT TCCACACCAC AAGCCTCTGC
    TTTGTCTGCA GCCACTTCGC TGCGGGACAA TCCCAAGTCA AAGAACGAAA TGATGATTTT
    GTAGAAATAG CGCGGAAGTT GAGTTTTCCA ATGGGAAGGC TGCTCTTCTC CCATGACTAT
    GTGTTTTGGT GTGGCGATTT CAACTACCGA ATCGATCTCC CTAATGAGGA AGTGAAAGAG
    CTTATCAGAC AGCAAAACTG GGATTCTCTT ATCGCAGGAG ATCAGCTTAT CAATCAGAAA
    AATGCTGGAC AGATTTTTAG AGGATTTTTA GAAGGAAAAG TGACCTTTGC TCCAACGTAT
    AAATACGACT TGTTTTCTGA TGACTACGAC ACTAGTGAAA AGTGCCGCAC CCCTGCATGG
    ACAGACCGTG TCCTCTGGAG AAGACGGAAG TGGCCTTTTG ATAGATCAGC TGAAGATTTA
    GATCTCCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATCC TCTACACATG GACTCCTGGC
    ACTTTGCTGC ACTACGGAAG GGCTGAGCTG AAGACTTCTG ACCATAGGCC TGTTGTTGCC
    CTGATCGATA TTGATATATT TGAAGTTGAA GCTGAAGAGA GGCAAAACAT TTATAAAGAA
    GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACGGTGTTGG TCTCAATCAA AAGCTCTTTA
    CCAGAAAATA ATTTTTTCAA CGATGCTTTG ATTGATGAGC TTTTACAGCA GTTTACAAAT
    TTCGGTGAAG TTATACTCAT AAGATTTGTG GAAGATAAAA TGTGGGTTAC GTTTTTAGAG
    GGAAGCTCTG CCTTGAATGT TCTGAACCTG AATGGGAAAG AGTTACTGGG TAGGACAATA
    ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG AAGAAGAAAT GAGCTTAGAG
    AAAATCAACG TCCCCTTGCC ATCATCAACC AGCTCCACCC TCCTAGGTGA AGACGCGGAG
    GTCACGGCCG ACTTCGATAT GGAAGGTGAT GTGGATGACT ATAGTGCTGA GGTAGAGGAG
    ATCCTTCCTC AGCATCTCCA GCCGTCTTCC AGTTCTGGCC TTGGCACGTC CCCCGGTTCT
    TCACCCCGGA CCAGTCCCTG CCAGTCACCT ACCATATCGG AGGGGCCTGT GCCTTCCCTC
    CCAGTGAGAC CAAGTCGAGC TCCGTCCAGA ACGCCCGGGC CCCCTGCTTC ACAAAGTTCT
    CCTGTTGACA CTCTGCCGGC AACACAGCTG CAGCAGAAAG ATTCTTCCCA GACCCTGGAA
    CCCAAGCGGC CTCCCCCTCC CCGCCCGGTT GCTCCTCCTG CACGTCCAGC TCCTCCACAA
    CGACCACCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCTG CTAGAAAAGA GTTTGGAGGT
    GTTGGAGCCC CTCCCAGTCC GGGGGTAGCT AGGCGAGAGA TGGAAGCACC CAAAAGCCCA
    GGAACGACCC GGAGAGATAA TCTAGGACGC AGCCAGCTTC CACCTCAAGC CGGACTGCCA
    GGCCCGGGAC TTGCTGGACA CAGTGCAGCC AGACCGATTA TTCCGCCCCG TGCTGGAGTC
    ATCAGCGCCC CCCAGAGCCA CGCACGGGTG TCTGCTGGGA GACTGACTCC TGAAAGCCAA
    AGCAAAACCT CAGAAGTACT GAAAGGGCCA GCTCTTCTTC CCGAGCCCCT GAAGCCTCAG
    GCCGCCCTTC CTGTGCCGCC TTCTCTTGCG CCACCTTCTC TTGCGCCACC TGTTCAGAAG
    ATGCAGGAGC CTCTCATCCC GGTGGCCGCA CCTCTGGCCC AGGCTGCCCT GCAGCCCAGC
    CTGGAAACGC CCCCGCAGCC ACCCCCTCGG AGCAGGTCGT CCCACAGCTT GCCTTCTGAG
    CCTCCGGCGC AGCCGCAGCA GGAGCAACCA TCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319516.1
    CDS29..4087
    Translation

    Target ORF information:

    RefSeq Version XM_015464030.1
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GTTAAAAGAT GGTGCTCGTT CAGTTAGTAG AACAATTCAG
    AATAACTTCT TTGACAGCTC CAAGCAAGAA GCAATTGATG TTTTGCTCCT GGGAAATACT
    CTAAATAGTG ATTTAGCTGA CAAAGCTCGA GCCCTTTTAA CTACTGGAAG TTTGCGTGTT
    TCTGAACAGA CGTTACAGTC AGCATCTTCT AAAGTACTAA AGAGTATGTG TGAGAATTTC
    TACAAGTATT CAAAGCCCAA GAAAATTCGA GTATGTGTGG GCACATGGAA CGTGAATGGC
    GGGAAGCAGT TTCGCAGCAT AGCTTTTAAG AATCAGACCC TTACGGACTG GCTTCTTGAT
    GCCCCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA GAAGTAAACC AATGGATATA
    TTTGCAATTG GTTTTGAGGA AATGGTAGAG CTGAATGCTG GAAACATTGT GAATGCAAGC
    ACAACAAATC AGAAGCTCTG GGCTGCGGAA CTTCAGAAGA CCATCTCCAG AGACAACAAG
    TATGTGCTGC TGGCCTCTGA GCAGTTGGTG GGCGTCTGTC TGTTTGTTTT TATCAGACCA
    CAGCACGCTC CCTTCATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG AATGGGAGGC
    GCAACTGGAA ATAAGGGAGC AGTTGCAATA CGAATGCTGT TCCACACCAC AAGCCTCTGC
    TTTGTCTGCA GCCACTTCGC TGCGGGACAA TCCCAAGTCA AAGAACGAAA TGATGATTTT
    GTAGAAATAG CGCGGAAGTT GAGTTTTCCA ATGGGAAGGC TGCTCTTCTC CCATGACTAT
    GTGTTTTGGT GTGGCGATTT CAACTACCGA ATCGATCTCC CTAATGAGGA AGTGAAAGAG
    CTTATCAGAC AGCAAAACTG GGATTCTCTT ATCGCAGGAG ATCAGCTTAT CAATCAGAAA
    AATGCTGGAC AGATTTTTAG AGGATTTTTA GAAGGAAAAG TGACCTTTGC TCCAACGTAT
    AAATACGACT TGTTTTCTGA TGACTACGAC ACTAGTGAAA AGTGCCGCAC CCCTGCATGG
    ACAGACCGTG TCCTCTGGAG AAGACGGAAG TGGCCTTTTG ATAGATCAGC TGAAGATTTA
    GATCTCCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATCC TCTACACATG GACTCCTGGC
    ACTTTGCTGC ACTACGGAAG GGCTGAGCTG AAGACTTCTG ACCATAGGCC TGTTGTTGCC
    CTGATCGATA TTGATATATT TGAAGTTGAA GCTGAAGAGA GGCAAAACAT TTATAAAGAA
    GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACGGTGTTGG TCTCAATCAA AAGCTCTTTA
    CCAGAAAATA ATTTTTTCAA CGATGCTTTG ATTGATGAGC TTTTACAGCA GTTTACAAAT
    TTCGGTGAAG TTATACTCAT AAGATTTGTG GAAGATAAAA TGTGGGTTAC GTTTTTAGAG
    GGAAGCTCTG CCTTGAATGT TCTGAACCTG AATGGGAAAG AGTTACTGGG TAGGACAATA
    ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG AAGAAGAAAT GAGCTTAGAG
    AAAATCAACG TCCCCTTGCC ATCATCAACC AGCTCCACCC TCCTAGGTGA AGACGCGGAG
    GTCACGGCCG ACTTCGATAT GGAAGGTGAT GTGGATGACT ATAGTGCTGA GGTAGAGGAG
    ATCCTTCCTC AGCATCTCCA GCCGTCTTCC AGTTCTGGCC TTGGCACGTC CCCCGGTTCT
    TCACCCCGGA CCAGTCCCTG CCAGTCACCT ACCATATCGG AGGGGCCTGT GCCTTCCCTC
    CCAGTGAGAC CAAGTCGAGC TCCGTCCAGA ACGCCCGGGC CCCCTGCTTC ACAAAGTTCT
    CCTGTTGACA CTCTGCCGGC AACACAGCTG CAGCAGAAAG ATTCTTCCCA GACCCTGGAA
    CCCAAGCGGC CTCCCCCTCC CCGCCCGGTT GCTCCTCCTG CACGTCCAGC TCCTCCACAA
    CGACCACCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCTG CTAGAAAAGA GTTTGGAGGT
    GTTGGAGCCC CTCCCAGTCC GGGGGTAGCT AGGCGAGAGA TGGAAGCACC CAAAAGCCCA
    GGAACGACCC GGAGAGATAA TCTAGGACGC AGCCAGCTTC CACCTCAAGC CGGACTGCCA
    GGCCCGGGAC TTGCTGGACA CAGTGCAGCC AGACCGATTA TTCCGCCCCG TGCTGGAGTC
    ATCAGCGCCC CCCAGAGCCA CGCACGGGTG TCTGCTGGGA GACTGACTCC TGAAAGCCAA
    AGCAAAACCT CAGAAGTACT GAAAGGGCCA GCTCTTCTTC CCGAGCCCCT GAAGCCTCAG
    GCCGCCCTTC CTGTGCCGCC TTCTCTTGCG CCACCTTCTC TTGCGCCACC TGTTCAGAAG
    ATGCAGGAGC CTCTCATCCC GGTGGCCGCA CCTCTGGCCC AGGCTGCCCT GCAGCCCAGC
    CTGGAAACGC CCCCGCAGCC ACCCCCTCGG AGCAGGTCGT CCCACAGCTT GCCTTCTGAG
    CCTCCGGCGC AGCCGCAGCA GGAGCAACCA TCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa44412
    Clone ID Related Accession (Same CDS sequence) NM_174468.3
    Accession Version NM_174468.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Bos taurus(cattle)
    Product synaptojanin-1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Jan 4, 2008 this sequence version replaced NM_174468.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: D85682.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145431, SAMN03145437 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGTTAAAAG ATGGTGCTCG TTCAGTTAGT
    AGAACAATTC AGAATAACTT CTTTGACAGC TCCAAGCAAG AAGCAATTGA TGTTTTGCTC
    CTGGGAAATA CTCTAAATAG TGATTTAGCT GACAAAGCTC GAGCCCTTTT AACTACTGGA
    AGTTTGCGTG TTTCTGAACA GACGTTACAG TCAGCATCTT CTAAAGTACT AAAGAGTATG
    TGTGAGAATT TCTACAAGTA TTCAAAGCCC AAGAAAATTC GAGTATGTGT GGGCACATGG
    AACGTGAATG GCGGGAAGCA GTTTCGCAGC ATAGCTTTTA AGAATCAGAC CCTTACGGAC
    TGGCTTCTTG ATGCCCCCAA GTTAGCTGGC ATCCAGGAGT TTCAAGATAA AAGAAGTAAA
    CCAATGGATA TATTTGCAAT TGGTTTTGAG GAAATGGTAG AGCTGAATGC TGGAAACATT
    GTGAATGCAA GCACAACAAA TCAGAAGCTC TGGGCTGCGG AACTTCAGAA GACCATCTCC
    AGAGACAACA AGTATGTGCT GCTGGCCTCT GAGCAGTTGG TGGGCGTCTG TCTGTTTGTT
    TTTATCAGAC CACAGCACGC TCCCTTCATC AGGGATGTTG CAGTTGATAC TGTGAAAACT
    GGAATGGGAG GCGCAACTGG AAATAAGGGA GCAGTTGCAA TACGAATGCT GTTCCACACC
    ACAAGCCTCT GCTTTGTCTG CAGCCACTTC GCTGCGGGAC AATCCCAAGT CAAAGAACGA
    AATGATGATT TTGTAGAAAT AGCGCGGAAG TTGAGTTTTC CAATGGGAAG GCTGCTCTTC
    TCCCATGACT ATGTGTTTTG GTGTGGCGAT TTCAACTACC GAATCGATCT CCCTAATGAG
    GAAGTGAAAG AGCTTATCAG ACAGCAAAAC TGGGATTCTC TTATCGCAGG AGATCAGCTT
    ATCAATCAGA AAAATGCTGG ACAGATTTTT AGAGGATTTT TAGAAGGAAA AGTGACCTTT
    GCTCCAACGT ATAAATACGA CTTGTTTTCT GATGACTACG ACACTAGTGA AAAGTGCCGC
    ACCCCTGCAT GGACAGACCG TGTCCTCTGG AGAAGACGGA AGTGGCCTTT TGATAGATCA
    GCTGAAGATT TAGATCTCCT AAATGCTAGT TTTCAAGATG AAAGCAAAAT CCTCTACACA
    TGGACTCCTG GCACTTTGCT GCACTACGGA AGGGCTGAGC TGAAGACTTC TGACCATAGG
    CCTGTTGTTG CCCTGATCGA TATTGATATA TTTGAAGTTG AAGCTGAAGA GAGGCAAAAC
    ATTTATAAAG AAGTAATTGC AGTTCAGGGT CCACCAGATG GTACGGTGTT GGTCTCAATC
    AAAAGCTCTT TACCAGAAAA TAATTTTTTC AACGATGCTT TGATTGATGA GCTTTTACAG
    CAGTTTACAA ATTTCGGTGA AGTTATACTC ATAAGATTTG TGGAAGATAA AATGTGGGTT
    ACGTTTTTAG AGGGAAGCTC TGCCTTGAAT GTTCTGAACC TGAATGGGAA AGAGTTACTG
    GGTAGGACAA TAACAATTAC TTTAAAAAGT CCAGACTGGA TCAAAACTTT GGAAGAAGAA
    ATGAGCTTAG AGAAAATCAA CGTCCCCTTG CCATCATCAA CCAGCTCCAC CCTCCTAGGT
    GAAGACGCGG AGGTCACGGC CGACTTCGAT ATGGAAGGTG ATGTGGATGA CTATAGTGCT
    GAGGTAGAGG AGATCCTTCC TCAGCATCTC CAGCCGTCTT CCAGTTCTGG CCTTGGCACG
    TCCCCCGGTT CTTCACCCCG GACCAGTCCC TGCCAGTCAC CTACCATATC GGAGGGGCCT
    GTGCCTTCCC TCCCAGTGAG ACCAAGTCGA GCTCCGTCCA GAACGCCCGG GCCCCCTGCT
    TCACAAAGTT CTCCTGTTGA CACTCTGCCG GCAACACAGC TGCAGCAGAA AGATTCTTCC
    CAGACCCTGG AACCCAAGCG GCCTCCCCCT CCCCGCCCGG TTGCTCCTCC TGCACGTCCA
    GCTCCTCCAC AACGACCACC TCCGCCTTCA GGGGCTAGGA GTCCTGCACC TGCTAGAAAA
    GAGTTTGGAG CACCCAAAAG CCCAGGAACG ACCCGGAGAG ATAATCTAGG ACGCAGCCAG
    CTTCCACCTC AAGCCGGACT GCCAGGCCCG GGACTTGCTG GACACAGTGC AGCCAGACCG
    ATTATTCCGC CCCGTGCTGG AGTCATCAGC GCCCCCCAGA GCCACGCACG GGTGTCTGCT
    GGGAGACTGA CTCCTGAAAG CCAAAGCAAA ACCTCAGAAG TACTGAAAGG GCCAGCTCTT
    CTTCCCGAGC CCCTGAAGCC TCAGGCCGCC CTTCCTGTGC CGCCTTCTCT TGCGCCACCT
    TCTCTTGCGC CACCTGTTCA GAAGATGCAG GAGCCTCTCA TCCCGGTGGC CGCACCTCTG
    GCCCAGGCTG CCCTGCAGCC CAGCCTGGAA ACGCCCCCGC AGCCACCCCC TCGGAGCAGG
    TCGTCCCACA GCTTGCCTTC TGAGCCTCCG GCGCAGCCGC AGCAGGAGCA ACCATCAGGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776893.2
    CDS1..3903
    Translation

    Target ORF information:

    RefSeq Version NM_174468.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174468.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGTTAAAAG ATGGTGCTCG TTCAGTTAGT
    AGAACAATTC AGAATAACTT CTTTGACAGC TCCAAGCAAG AAGCAATTGA TGTTTTGCTC
    CTGGGAAATA CTCTAAATAG TGATTTAGCT GACAAAGCTC GAGCCCTTTT AACTACTGGA
    AGTTTGCGTG TTTCTGAACA GACGTTACAG TCAGCATCTT CTAAAGTACT AAAGAGTATG
    TGTGAGAATT TCTACAAGTA TTCAAAGCCC AAGAAAATTC GAGTATGTGT GGGCACATGG
    AACGTGAATG GCGGGAAGCA GTTTCGCAGC ATAGCTTTTA AGAATCAGAC CCTTACGGAC
    TGGCTTCTTG ATGCCCCCAA GTTAGCTGGC ATCCAGGAGT TTCAAGATAA AAGAAGTAAA
    CCAATGGATA TATTTGCAAT TGGTTTTGAG GAAATGGTAG AGCTGAATGC TGGAAACATT
    GTGAATGCAA GCACAACAAA TCAGAAGCTC TGGGCTGCGG AACTTCAGAA GACCATCTCC
    AGAGACAACA AGTATGTGCT GCTGGCCTCT GAGCAGTTGG TGGGCGTCTG TCTGTTTGTT
    TTTATCAGAC CACAGCACGC TCCCTTCATC AGGGATGTTG CAGTTGATAC TGTGAAAACT
    GGAATGGGAG GCGCAACTGG AAATAAGGGA GCAGTTGCAA TACGAATGCT GTTCCACACC
    ACAAGCCTCT GCTTTGTCTG CAGCCACTTC GCTGCGGGAC AATCCCAAGT CAAAGAACGA
    AATGATGATT TTGTAGAAAT AGCGCGGAAG TTGAGTTTTC CAATGGGAAG GCTGCTCTTC
    TCCCATGACT ATGTGTTTTG GTGTGGCGAT TTCAACTACC GAATCGATCT CCCTAATGAG
    GAAGTGAAAG AGCTTATCAG ACAGCAAAAC TGGGATTCTC TTATCGCAGG AGATCAGCTT
    ATCAATCAGA AAAATGCTGG ACAGATTTTT AGAGGATTTT TAGAAGGAAA AGTGACCTTT
    GCTCCAACGT ATAAATACGA CTTGTTTTCT GATGACTACG ACACTAGTGA AAAGTGCCGC
    ACCCCTGCAT GGACAGACCG TGTCCTCTGG AGAAGACGGA AGTGGCCTTT TGATAGATCA
    GCTGAAGATT TAGATCTCCT AAATGCTAGT TTTCAAGATG AAAGCAAAAT CCTCTACACA
    TGGACTCCTG GCACTTTGCT GCACTACGGA AGGGCTGAGC TGAAGACTTC TGACCATAGG
    CCTGTTGTTG CCCTGATCGA TATTGATATA TTTGAAGTTG AAGCTGAAGA GAGGCAAAAC
    ATTTATAAAG AAGTAATTGC AGTTCAGGGT CCACCAGATG GTACGGTGTT GGTCTCAATC
    AAAAGCTCTT TACCAGAAAA TAATTTTTTC AACGATGCTT TGATTGATGA GCTTTTACAG
    CAGTTTACAA ATTTCGGTGA AGTTATACTC ATAAGATTTG TGGAAGATAA AATGTGGGTT
    ACGTTTTTAG AGGGAAGCTC TGCCTTGAAT GTTCTGAACC TGAATGGGAA AGAGTTACTG
    GGTAGGACAA TAACAATTAC TTTAAAAAGT CCAGACTGGA TCAAAACTTT GGAAGAAGAA
    ATGAGCTTAG AGAAAATCAA CGTCCCCTTG CCATCATCAA CCAGCTCCAC CCTCCTAGGT
    GAAGACGCGG AGGTCACGGC CGACTTCGAT ATGGAAGGTG ATGTGGATGA CTATAGTGCT
    GAGGTAGAGG AGATCCTTCC TCAGCATCTC CAGCCGTCTT CCAGTTCTGG CCTTGGCACG
    TCCCCCGGTT CTTCACCCCG GACCAGTCCC TGCCAGTCAC CTACCATATC GGAGGGGCCT
    GTGCCTTCCC TCCCAGTGAG ACCAAGTCGA GCTCCGTCCA GAACGCCCGG GCCCCCTGCT
    TCACAAAGTT CTCCTGTTGA CACTCTGCCG GCAACACAGC TGCAGCAGAA AGATTCTTCC
    CAGACCCTGG AACCCAAGCG GCCTCCCCCT CCCCGCCCGG TTGCTCCTCC TGCACGTCCA
    GCTCCTCCAC AACGACCACC TCCGCCTTCA GGGGCTAGGA GTCCTGCACC TGCTAGAAAA
    GAGTTTGGAG CACCCAAAAG CCCAGGAACG ACCCGGAGAG ATAATCTAGG ACGCAGCCAG
    CTTCCACCTC AAGCCGGACT GCCAGGCCCG GGACTTGCTG GACACAGTGC AGCCAGACCG
    ATTATTCCGC CCCGTGCTGG AGTCATCAGC GCCCCCCAGA GCCACGCACG GGTGTCTGCT
    GGGAGACTGA CTCCTGAAAG CCAAAGCAAA ACCTCAGAAG TACTGAAAGG GCCAGCTCTT
    CTTCCCGAGC CCCTGAAGCC TCAGGCCGCC CTTCCTGTGC CGCCTTCTCT TGCGCCACCT
    TCTCTTGCGC CACCTGTTCA GAAGATGCAG GAGCCTCTCA TCCCGGTGGC CGCACCTCTG
    GCCCAGGCTG CCCTGCAGCC CAGCCTGGAA ACGCCCCCGC AGCCACCCCC TCGGAGCAGG
    TCGTCCCACA GCTTGCCTTC TGAGCCTCCG GCGCAGCCGC AGCAGGAGCA ACCATCAGGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45338
    Clone ID Related Accession (Same CDS sequence) XM_005201097.4 , XM_005201097.3
    Accession Version XM_005201097.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201097.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGACGCA GCCAGCTTCC ACCTCAAGCC GGACTGCCAG GCCCGGGACT TGCTGGACAC
    AGTGCAGCCA GACCGATTAT TCCGCCCCGT GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC
    GCACGGGTGT CTGCTGGGAG ACTGACTCCT GAAAGCCAAA GCAAAACCTC AGAAGTACTG
    AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT
    TCTCTTGCGC CACCTTCTCT TGCGCCACCT GTTCAGAAGA TGCAGGAGCC TCTCATCCCG
    GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG CAGCCCAGCC TGGAAACGCC CCCGCAGCCA
    CCCCCTCGGA GCAGGTCGTC CCACAGCTTG CCTTCTGAGC CTCCGGCGCA GCCGCAGCAG
    GAGCAACCAT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201154.2
    CDS16..3933
    Translation

    Target ORF information:

    RefSeq Version XM_005201097.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201097.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGACGCA GCCAGCTTCC ACCTCAAGCC GGACTGCCAG GCCCGGGACT TGCTGGACAC
    AGTGCAGCCA GACCGATTAT TCCGCCCCGT GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC
    GCACGGGTGT CTGCTGGGAG ACTGACTCCT GAAAGCCAAA GCAAAACCTC AGAAGTACTG
    AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT
    TCTCTTGCGC CACCTTCTCT TGCGCCACCT GTTCAGAAGA TGCAGGAGCC TCTCATCCCG
    GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG CAGCCCAGCC TGGAAACGCC CCCGCAGCCA
    CCCCCTCGGA GCAGGTCGTC CCACAGCTTG CCTTCTGAGC CTCCGGCGCA GCCGCAGCAG
    GAGCAACCAT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45338
    Clone ID Related Accession (Same CDS sequence) XM_005201097.4 , XM_005201097.3
    Accession Version XM_005201097.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGACGCA GCCAGCTTCC ACCTCAAGCC GGACTGCCAG GCCCGGGACT TGCTGGACAC
    AGTGCAGCCA GACCGATTAT TCCGCCCCGT GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC
    GCACGGGTGT CTGCTGGGAG ACTGACTCCT GAAAGCCAAA GCAAAACCTC AGAAGTACTG
    AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT
    TCTCTTGCGC CACCTTCTCT TGCGCCACCT GTTCAGAAGA TGCAGGAGCC TCTCATCCCG
    GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG CAGCCCAGCC TGGAAACGCC CCCGCAGCCA
    CCCCCTCGGA GCAGGTCGTC CCACAGCTTG CCTTCTGAGC CTCCGGCGCA GCCGCAGCAG
    GAGCAACCAT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201154.2
    CDS29..3946
    Translation

    Target ORF information:

    RefSeq Version XM_005201097.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201097.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGACGCA GCCAGCTTCC ACCTCAAGCC GGACTGCCAG GCCCGGGACT TGCTGGACAC
    AGTGCAGCCA GACCGATTAT TCCGCCCCGT GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC
    GCACGGGTGT CTGCTGGGAG ACTGACTCCT GAAAGCCAAA GCAAAACCTC AGAAGTACTG
    AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT
    TCTCTTGCGC CACCTTCTCT TGCGCCACCT GTTCAGAAGA TGCAGGAGCC TCTCATCCCG
    GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG CAGCCCAGCC TGGAAACGCC CCCGCAGCCA
    CCCCCTCGGA GCAGGTCGTC CCACAGCTTG CCTTCTGAGC CTCCGGCGCA GCCGCAGCAG
    GAGCAACCAT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218601
    Clone ID Related Accession (Same CDS sequence) XM_024991867.1
    Accession Version XM_024991867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGGGC TAGGAGTCCT GCACCTGCTA GAAAAGAGTT TGGAGGTGTT
    GGAGCCCCTC CCAGTCCGGG GGTAGCTAGG CGAGAGATGG AAGCACCCAA AAGCCCAGGA
    ACGACCCGGA GAGATAATCT AGGACGCAGC CAGCTTCCAC CTCAAGCCGG ACTGCCAGGC
    CCGGGACTTG CTGGACACAG TGCAGCCAGA CCGATTATTC CGCCCCGTGC TGGAGTCATC
    AGCGCCCCCC AGAGCCACGC ACGGGTGTCT GCTGGGAGAC TGACTCCTGA AAGCCAAAGC
    AAAACCTCAG AAGTACTGAA AGGGCCAGCT CTTCTTCCCG AGCCCCTGAA GCCTCAGGCC
    GCCCTTCCTG TGCCGCCTTC TCTTGCGCCA CCTTCTCTTG CGCCACCTGT TCAGAAGATG
    CAGGAGCCTC TCATCCCGGT GGCCGCACCT CTGGCCCAGG CTGCCCTGCA GCCCAGCCTG
    GAAACGCCCC CGCAGCCACC CCCTCGGAGC AGGTCGTCCC ACAGCTTGCC TTCTGAGCCT
    CCGGCGCAGC CGCAGGTGAA AACAAACGGA GTCTCCGCCG TCAGACTGGA CTCGCCATTA
    AAGAGTGACC CATTTGAAGA CTTGTCATTG AACCTGCTTG CTGTATCAAA GGCTCAGCCA
    TCTGTTCACA CCCCAAACCC AAGGGGGTTG ACGCCGTTGC CTTCTGCAGC CCCAAGTAAC
    ACCAACACTC TGAGTTCTGT AAGCTGCATG CCGACAATGC CTCCAATTCC AGCCCGGAGT
    AAATCCCAGG AAAACACGCG ATGTTCCCCA AACCCATTCA TCCCAAGCTC AAGCAGCACA
    AATCCTTTCA CCGACAGGAC CGCCGCTCCT GGAAACCCCT TTCGAGCTGA GTCTCAAGAA
    TCAGAGGCCA CTTCATGGTT CTCCAAAGAA GAGCCTGTTG CTCCGAGTCC ATTCTCTTCG
    CTGAGGCCTC TGGATCAGAA CAGTAGCAAG CCTTCATCCT CCCTGGATGG GTTTAAGGAC
    AGTTTTGATC CACAGGGCCT GTCTGCACTA ACAGTCAGCA ACCCCAAAGG ATGGGTAACC
    TTCGAGGAAG AAGAGGACTT TGGTGTGACA GGGAAGTCAG GGTCCACTCG CCCAGACGTT
    TTCCTGGGTA AGCAGCTGAG CTCGTCTCCT GGCTCCAAGG TGATGCTTGG TGATGACTGG
    GGTAGGAGTA CCAATGTGTC TCTCTGTGTG TTGCCAGCAA GACCACCACC CCCACCGCCT
    GTCCCTCTGC TCCCACCTGG CACCACCCCT CCCGCAGACG CTTTCACAGC CCTGGCCTCT
    AAGGCATCAC CCACGCTAGA CTTGACAGAA AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847635.1
    CDS149..4864
    Translation

    Target ORF information:

    RefSeq Version XM_024991867.1
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGGGC TAGGAGTCCT GCACCTGCTA GAAAAGAGTT TGGAGGTGTT
    GGAGCCCCTC CCAGTCCGGG GGTAGCTAGG CGAGAGATGG AAGCACCCAA AAGCCCAGGA
    ACGACCCGGA GAGATAATCT AGGACGCAGC CAGCTTCCAC CTCAAGCCGG ACTGCCAGGC
    CCGGGACTTG CTGGACACAG TGCAGCCAGA CCGATTATTC CGCCCCGTGC TGGAGTCATC
    AGCGCCCCCC AGAGCCACGC ACGGGTGTCT GCTGGGAGAC TGACTCCTGA AAGCCAAAGC
    AAAACCTCAG AAGTACTGAA AGGGCCAGCT CTTCTTCCCG AGCCCCTGAA GCCTCAGGCC
    GCCCTTCCTG TGCCGCCTTC TCTTGCGCCA CCTTCTCTTG CGCCACCTGT TCAGAAGATG
    CAGGAGCCTC TCATCCCGGT GGCCGCACCT CTGGCCCAGG CTGCCCTGCA GCCCAGCCTG
    GAAACGCCCC CGCAGCCACC CCCTCGGAGC AGGTCGTCCC ACAGCTTGCC TTCTGAGCCT
    CCGGCGCAGC CGCAGGTGAA AACAAACGGA GTCTCCGCCG TCAGACTGGA CTCGCCATTA
    AAGAGTGACC CATTTGAAGA CTTGTCATTG AACCTGCTTG CTGTATCAAA GGCTCAGCCA
    TCTGTTCACA CCCCAAACCC AAGGGGGTTG ACGCCGTTGC CTTCTGCAGC CCCAAGTAAC
    ACCAACACTC TGAGTTCTGT AAGCTGCATG CCGACAATGC CTCCAATTCC AGCCCGGAGT
    AAATCCCAGG AAAACACGCG ATGTTCCCCA AACCCATTCA TCCCAAGCTC AAGCAGCACA
    AATCCTTTCA CCGACAGGAC CGCCGCTCCT GGAAACCCCT TTCGAGCTGA GTCTCAAGAA
    TCAGAGGCCA CTTCATGGTT CTCCAAAGAA GAGCCTGTTG CTCCGAGTCC ATTCTCTTCG
    CTGAGGCCTC TGGATCAGAA CAGTAGCAAG CCTTCATCCT CCCTGGATGG GTTTAAGGAC
    AGTTTTGATC CACAGGGCCT GTCTGCACTA ACAGTCAGCA ACCCCAAAGG ATGGGTAACC
    TTCGAGGAAG AAGAGGACTT TGGTGTGACA GGGAAGTCAG GGTCCACTCG CCCAGACGTT
    TTCCTGGGTA AGCAGCTGAG CTCGTCTCCT GGCTCCAAGG TGATGCTTGG TGATGACTGG
    GGTAGGAGTA CCAATGTGTC TCTCTGTGTG TTGCCAGCAA GACCACCACC CCCACCGCCT
    GTCCCTCTGC TCCCACCTGG CACCACCCCT CCCGCAGACG CTTTCACAGC CCTGGCCTCT
    AAGGCATCAC CCACGCTAGA CTTGACAGAA AGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218602
    Clone ID Related Accession (Same CDS sequence) XM_024991891.1 , XM_024991927.1 , XM_024991959.1
    Accession Version XM_024991891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGACG CAGCCAGCTT CCACCTCAAG CCGGACTGCC AGGCCCGGGA
    CTTGCTGGAC ACAGTGCAGC CAGACCGATT ATTCCGCCCC GTGCTGGAGT CATCAGCGCC
    CCCCAGAGCC ACGCACGGGT GTCTGCTGGG AGACTGACTC CTGAAAGCCA AAGCAAAACC
    TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT
    CCTGTGCCGC CTTCTCTTGC GCCACCTTCT CTTGCGCCAC CTGTTCAGAA GATGCAGGAG
    CCTCTCATCC CGGTGGCCGC ACCTCTGGCC CAGGCTGCCC TGCAGCCCAG CCTGGAAACG
    CCCCCGCAGC CACCCCCTCG GAGCAGGTCG TCCCACAGCT TGCCTTCTGA GCCTCCGGCG
    CAGCCGCAGG TGAAAACAAA CGGAGTCTCC GCCGTCAGAC TGGACTCGCC ATTAAAGAGT
    GACCCATTTG AAGACTTGTC ATTGAACCTG CTTGCTGTAT CAAAGGCTCA GCCATCTGTT
    CACACCCCAA ACCCAAGGGG GTTGACGCCG TTGCCTTCTG CAGCCCCAAG TAACACCAAC
    ACTCTGAGTT CTGTAAGCTG CATGCCGACA ATGCCTCCAA TTCCAGCCCG GAGTAAATCC
    CAGGAAAACA CGCGATGTTC CCCAAACCCA TTCATCCCAA GCTCAAGCAG CACAAATCCT
    TTCACCGACA GGACCGCCGC TCCTGGAAAC CCCTTTCGAG CTGAGTCTCA AGAATCAGAG
    GCCACTTCAT GGTTCTCCAA AGAAGAGCCT GTTGCTCCGA GTCCATTCTC TTCGCTGAGG
    CCTCTGGATC AGAACAGTAG CAAGCCTTCA TCCTCCCTGG ATGGGTTTAA GGACAGTTTT
    GATCCACAGG GCCTGTCTGC ACTAACAGTC AGCAACCCCA AAGGATGGGT AACCTTCGAG
    GAAGAAGAGG ACTTTGGTGT GACAGGGAAG TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG
    GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC AAGGTGATGC TTGGTGATGA CTGGGGTAGG
    AGTACCAATG TGTCTCTCTG TGTGTTGCCA GCAAGACCAC CACCCCCACC GCCTGTCCCT
    CTGCTCCCAC CTGGCACCAC CCCTCCCGCA GACGCTTTCA CAGCCCTGGC CTCTAAGGCA
    TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847659.1
    CDS149..4738
    Translation

    Target ORF information:

    RefSeq Version XM_024991891.1
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGACG CAGCCAGCTT CCACCTCAAG CCGGACTGCC AGGCCCGGGA
    CTTGCTGGAC ACAGTGCAGC CAGACCGATT ATTCCGCCCC GTGCTGGAGT CATCAGCGCC
    CCCCAGAGCC ACGCACGGGT GTCTGCTGGG AGACTGACTC CTGAAAGCCA AAGCAAAACC
    TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT
    CCTGTGCCGC CTTCTCTTGC GCCACCTTCT CTTGCGCCAC CTGTTCAGAA GATGCAGGAG
    CCTCTCATCC CGGTGGCCGC ACCTCTGGCC CAGGCTGCCC TGCAGCCCAG CCTGGAAACG
    CCCCCGCAGC CACCCCCTCG GAGCAGGTCG TCCCACAGCT TGCCTTCTGA GCCTCCGGCG
    CAGCCGCAGG TGAAAACAAA CGGAGTCTCC GCCGTCAGAC TGGACTCGCC ATTAAAGAGT
    GACCCATTTG AAGACTTGTC ATTGAACCTG CTTGCTGTAT CAAAGGCTCA GCCATCTGTT
    CACACCCCAA ACCCAAGGGG GTTGACGCCG TTGCCTTCTG CAGCCCCAAG TAACACCAAC
    ACTCTGAGTT CTGTAAGCTG CATGCCGACA ATGCCTCCAA TTCCAGCCCG GAGTAAATCC
    CAGGAAAACA CGCGATGTTC CCCAAACCCA TTCATCCCAA GCTCAAGCAG CACAAATCCT
    TTCACCGACA GGACCGCCGC TCCTGGAAAC CCCTTTCGAG CTGAGTCTCA AGAATCAGAG
    GCCACTTCAT GGTTCTCCAA AGAAGAGCCT GTTGCTCCGA GTCCATTCTC TTCGCTGAGG
    CCTCTGGATC AGAACAGTAG CAAGCCTTCA TCCTCCCTGG ATGGGTTTAA GGACAGTTTT
    GATCCACAGG GCCTGTCTGC ACTAACAGTC AGCAACCCCA AAGGATGGGT AACCTTCGAG
    GAAGAAGAGG ACTTTGGTGT GACAGGGAAG TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG
    GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC AAGGTGATGC TTGGTGATGA CTGGGGTAGG
    AGTACCAATG TGTCTCTCTG TGTGTTGCCA GCAAGACCAC CACCCCCACC GCCTGTCCCT
    CTGCTCCCAC CTGGCACCAC CCCTCCCGCA GACGCTTTCA CAGCCCTGGC CTCTAAGGCA
    TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218602
    Clone ID Related Accession (Same CDS sequence) XM_024991891.1 , XM_024991927.1 , XM_024991959.1
    Accession Version XM_024991927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGACG CAGCCAGCTT CCACCTCAAG CCGGACTGCC AGGCCCGGGA
    CTTGCTGGAC ACAGTGCAGC CAGACCGATT ATTCCGCCCC GTGCTGGAGT CATCAGCGCC
    CCCCAGAGCC ACGCACGGGT GTCTGCTGGG AGACTGACTC CTGAAAGCCA AAGCAAAACC
    TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT
    CCTGTGCCGC CTTCTCTTGC GCCACCTTCT CTTGCGCCAC CTGTTCAGAA GATGCAGGAG
    CCTCTCATCC CGGTGGCCGC ACCTCTGGCC CAGGCTGCCC TGCAGCCCAG CCTGGAAACG
    CCCCCGCAGC CACCCCCTCG GAGCAGGTCG TCCCACAGCT TGCCTTCTGA GCCTCCGGCG
    CAGCCGCAGG TGAAAACAAA CGGAGTCTCC GCCGTCAGAC TGGACTCGCC ATTAAAGAGT
    GACCCATTTG AAGACTTGTC ATTGAACCTG CTTGCTGTAT CAAAGGCTCA GCCATCTGTT
    CACACCCCAA ACCCAAGGGG GTTGACGCCG TTGCCTTCTG CAGCCCCAAG TAACACCAAC
    ACTCTGAGTT CTGTAAGCTG CATGCCGACA ATGCCTCCAA TTCCAGCCCG GAGTAAATCC
    CAGGAAAACA CGCGATGTTC CCCAAACCCA TTCATCCCAA GCTCAAGCAG CACAAATCCT
    TTCACCGACA GGACCGCCGC TCCTGGAAAC CCCTTTCGAG CTGAGTCTCA AGAATCAGAG
    GCCACTTCAT GGTTCTCCAA AGAAGAGCCT GTTGCTCCGA GTCCATTCTC TTCGCTGAGG
    CCTCTGGATC AGAACAGTAG CAAGCCTTCA TCCTCCCTGG ATGGGTTTAA GGACAGTTTT
    GATCCACAGG GCCTGTCTGC ACTAACAGTC AGCAACCCCA AAGGATGGGT AACCTTCGAG
    GAAGAAGAGG ACTTTGGTGT GACAGGGAAG TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG
    GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC AAGGTGATGC TTGGTGATGA CTGGGGTAGG
    AGTACCAATG TGTCTCTCTG TGTGTTGCCA GCAAGACCAC CACCCCCACC GCCTGTCCCT
    CTGCTCCCAC CTGGCACCAC CCCTCCCGCA GACGCTTTCA CAGCCCTGGC CTCTAAGGCA
    TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847695.1
    CDS229..4818
    Translation

    Target ORF information:

    RefSeq Version XM_024991927.1
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGACG CAGCCAGCTT CCACCTCAAG CCGGACTGCC AGGCCCGGGA
    CTTGCTGGAC ACAGTGCAGC CAGACCGATT ATTCCGCCCC GTGCTGGAGT CATCAGCGCC
    CCCCAGAGCC ACGCACGGGT GTCTGCTGGG AGACTGACTC CTGAAAGCCA AAGCAAAACC
    TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT
    CCTGTGCCGC CTTCTCTTGC GCCACCTTCT CTTGCGCCAC CTGTTCAGAA GATGCAGGAG
    CCTCTCATCC CGGTGGCCGC ACCTCTGGCC CAGGCTGCCC TGCAGCCCAG CCTGGAAACG
    CCCCCGCAGC CACCCCCTCG GAGCAGGTCG TCCCACAGCT TGCCTTCTGA GCCTCCGGCG
    CAGCCGCAGG TGAAAACAAA CGGAGTCTCC GCCGTCAGAC TGGACTCGCC ATTAAAGAGT
    GACCCATTTG AAGACTTGTC ATTGAACCTG CTTGCTGTAT CAAAGGCTCA GCCATCTGTT
    CACACCCCAA ACCCAAGGGG GTTGACGCCG TTGCCTTCTG CAGCCCCAAG TAACACCAAC
    ACTCTGAGTT CTGTAAGCTG CATGCCGACA ATGCCTCCAA TTCCAGCCCG GAGTAAATCC
    CAGGAAAACA CGCGATGTTC CCCAAACCCA TTCATCCCAA GCTCAAGCAG CACAAATCCT
    TTCACCGACA GGACCGCCGC TCCTGGAAAC CCCTTTCGAG CTGAGTCTCA AGAATCAGAG
    GCCACTTCAT GGTTCTCCAA AGAAGAGCCT GTTGCTCCGA GTCCATTCTC TTCGCTGAGG
    CCTCTGGATC AGAACAGTAG CAAGCCTTCA TCCTCCCTGG ATGGGTTTAA GGACAGTTTT
    GATCCACAGG GCCTGTCTGC ACTAACAGTC AGCAACCCCA AAGGATGGGT AACCTTCGAG
    GAAGAAGAGG ACTTTGGTGT GACAGGGAAG TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG
    GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC AAGGTGATGC TTGGTGATGA CTGGGGTAGG
    AGTACCAATG TGTCTCTCTG TGTGTTGCCA GCAAGACCAC CACCCCCACC GCCTGTCCCT
    CTGCTCCCAC CTGGCACCAC CCCTCCCGCA GACGCTTTCA CAGCCCTGGC CTCTAAGGCA
    TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218602
    Clone ID Related Accession (Same CDS sequence) XM_024991891.1 , XM_024991927.1 , XM_024991959.1
    Accession Version XM_024991959.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGACG CAGCCAGCTT CCACCTCAAG CCGGACTGCC AGGCCCGGGA
    CTTGCTGGAC ACAGTGCAGC CAGACCGATT ATTCCGCCCC GTGCTGGAGT CATCAGCGCC
    CCCCAGAGCC ACGCACGGGT GTCTGCTGGG AGACTGACTC CTGAAAGCCA AAGCAAAACC
    TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT
    CCTGTGCCGC CTTCTCTTGC GCCACCTTCT CTTGCGCCAC CTGTTCAGAA GATGCAGGAG
    CCTCTCATCC CGGTGGCCGC ACCTCTGGCC CAGGCTGCCC TGCAGCCCAG CCTGGAAACG
    CCCCCGCAGC CACCCCCTCG GAGCAGGTCG TCCCACAGCT TGCCTTCTGA GCCTCCGGCG
    CAGCCGCAGG TGAAAACAAA CGGAGTCTCC GCCGTCAGAC TGGACTCGCC ATTAAAGAGT
    GACCCATTTG AAGACTTGTC ATTGAACCTG CTTGCTGTAT CAAAGGCTCA GCCATCTGTT
    CACACCCCAA ACCCAAGGGG GTTGACGCCG TTGCCTTCTG CAGCCCCAAG TAACACCAAC
    ACTCTGAGTT CTGTAAGCTG CATGCCGACA ATGCCTCCAA TTCCAGCCCG GAGTAAATCC
    CAGGAAAACA CGCGATGTTC CCCAAACCCA TTCATCCCAA GCTCAAGCAG CACAAATCCT
    TTCACCGACA GGACCGCCGC TCCTGGAAAC CCCTTTCGAG CTGAGTCTCA AGAATCAGAG
    GCCACTTCAT GGTTCTCCAA AGAAGAGCCT GTTGCTCCGA GTCCATTCTC TTCGCTGAGG
    CCTCTGGATC AGAACAGTAG CAAGCCTTCA TCCTCCCTGG ATGGGTTTAA GGACAGTTTT
    GATCCACAGG GCCTGTCTGC ACTAACAGTC AGCAACCCCA AAGGATGGGT AACCTTCGAG
    GAAGAAGAGG ACTTTGGTGT GACAGGGAAG TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG
    GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC AAGGTGATGC TTGGTGATGA CTGGGGTAGG
    AGTACCAATG TGTCTCTCTG TGTGTTGCCA GCAAGACCAC CACCCCCACC GCCTGTCCCT
    CTGCTCCCAC CTGGCACCAC CCCTCCCGCA GACGCTTTCA CAGCCCTGGC CTCTAAGGCA
    TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847727.1
    CDS110..4699
    Translation

    Target ORF information:

    RefSeq Version XM_024991959.1
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991959.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGTTCA GCAAAGGATT TCGGATCTAT CACAAATTGG ATCCCCCACC TTTCAGCCTC 
    ATAGTGGAAA CCAGGCATAA GGAAGAATGT CTCATGTTCG AGTCTGGGGC TGTAGCGGTG
    CTCTCATCAG CAGAAAAAGA GGCAATCAAG GGTACATACT CCAAAGTTCT AGATGCCTAT
    GGACTTTTAG GTGTTTTACG ATTAAATCTT GGTGATATTA TGTTACATTA TCTGGTCCTA
    GTCACTGGAT GTATGTCTGT TGGAAAAATT CAAGAATCTG AAGTTTTCCG AGTTACTTCC
    ACTGAATTTA TATCACTGCG AGTTGATTCT TCGGATGAGG ATCGCATTTC GGAAGTGCGA
    AAAGTTTTAA ATTCAGGAAA CTTTTATTTT GCATGGTCTG CATCCGGAGT CAGTTTGGAT
    CTGAGTCTTA ATGCCCACCG TAGCTTGCAA GAACACACAA CTGACAATAG ATTTTTCTGG
    AATCAGTCTT TGCACTTGCA TCTCAAGCAC TACGGTGTGA ATTGTGACGA CTGGTTATTA
    CGCCTCATGT GTGGGGGAGT AGAAATCAGA ACAATTTATG CTGCTCATAA ACAGGCAAAG
    GCTTGCCTCA TTTCCAGACT AAGCTGTGAA CGAGCTGGGA CCAGGTTTAA CGTCCGGGGA
    ACAAATGATG ATGGTCACGT TGCCAATTTT GTAGAAACAG AACAGGTTGT GTACTTAGAT
    GACTCTGTTT CTTCCTTCAT ACAAATCCGG GGATCTGTCC CATTGTTCTG GGAGCAGCCA
    GGATTGCAGG TGGGATCTCA TCGTGTCCGT ATGTCAAGGG GATTTGAAGC CAATGCACCT
    GCTTTTGACA GGCATTTTAG GACACTTAAG AATTTGTATG GTAAACAAAT AATAGTAAAT
    CTGCTTGGAT CTAAGGAAGG TGAACATATG CTAAGTAAGG CTTTCCAGAG TCATCTGAAA
    GCTTCTGAAC ATGCTGCTGA TATCCAGATG GTGAATTTTG ACTATCATCA AATGGTTAAA
    GGAGGAAAGG CAGAAAAATT ACATAGTGTT CTTAAACCTC AAGTCCAGAA GTTTCTGGAT
    TATGGAATTT TTCATTTTGA TGGAAGTGAA GTTCAAAGGT GCCAGAGTGG TACAGTTCGA
    ACAAACTGCT TGGATTGTCT TGACAGAACA AATAGTGTGC AGGCATTCCT TGGTTTAGAG
    ATGCTGACTA AACAGTTGGA AGCTCTTGGT TTAGCTGAAA AGCCTCAGTT GGTGACTCGC
    TTTCAAGAAG TTTTTCGATC AATGTGGTCT GTGAATGGTG ATTCAATCAG TAAGATTTAT
    GCAGGAACTG GAGCCCTTGA AGGAAAGGCT AAGGCTGGAA AGTTAAAAGA TGGTGCTCGT
    TCAGTTAGTA GAACAATTCA GAATAACTTC TTTGACAGCT CCAAGCAAGA AGCAATTGAT
    GTTTTGCTCC TGGGAAATAC TCTAAATAGT GATTTAGCTG ACAAAGCTCG AGCCCTTTTA
    ACTACTGGAA GTTTGCGTGC ATCTTCTAAA GTACTAAAGA GTATGTGTGA GAATTTCTAC
    AAGTATTCAA AGCCCAAGAA AATTCGAGTA TGTGTGGGCA CATGGAACGT GAATGGCGGG
    AAGCAGTTTC GCAGCATAGC TTTTAAGAAT CAGACCCTTA CGGACTGGCT TCTTGATGCC
    CCCAAGTTAG CTGGCATCCA GGAGTTTCAA GATAAAAGAA GTAAACCAAT GGATATATTT
    GCAATTGGTT TTGAGGAAAT GGTAGAGCTG AATGCTGGAA ACATTGTGAA TGCAAGCACA
    ACAAATCAGA AGCTCTGGGC TGCGGAACTT CAGAAGACCA TCTCCAGAGA CAACAAGTAT
    GTGCTGCTGG CCTCTGAGCA GTTGGTGGGC GTCTGTCTGT TTGTTTTTAT CAGACCACAG
    CACGCTCCCT TCATCAGGGA TGTTGCAGTT GATACTGTGA AAACTGGAAT GGGAGGCGCA
    ACTGGAAATA AGGGAGCAGT TGCAATACGA ATGCTGTTCC ACACCACAAG CCTCTGCTTT
    GTCTGCAGCC ACTTCGCTGC GGGACAATCC CAAGTCAAAG AACGAAATGA TGATTTTGTA
    GAAATAGCGC GGAAGTTGAG TTTTCCAATG GGAAGGCTGC TCTTCTCCCA TGACTATGTG
    TTTTGGTGTG GCGATTTCAA CTACCGAATC GATCTCCCTA ATGAGGAAGT GAAAGAGCTT
    ATCAGACAGC AAAACTGGGA TTCTCTTATC GCAGGAGATC AGCTTATCAA TCAGAAAAAT
    GCTGGACAGA TTTTTAGAGG ATTTTTAGAA GGAAAAGTGA CCTTTGCTCC AACGTATAAA
    TACGACTTGT TTTCTGATGA CTACGACACT AGTGAAAAGT GCCGCACCCC TGCATGGACA
    GACCGTGTCC TCTGGAGAAG ACGGAAGTGG CCTTTTGATA GATCAGCTGA AGATTTAGAT
    CTCCTAAATG CTAGTTTTCA AGATGAAAGC AAAATCCTCT ACACATGGAC TCCTGGCACT
    TTGCTGCACT ACGGAAGGGC TGAGCTGAAG ACTTCTGACC ATAGGCCTGT TGTTGCCCTG
    ATCGATATTG ATATATTTGA AGTTGAAGCT GAAGAGAGGC AAAACATTTA TAAAGAAGTA
    ATTGCAGTTC AGGGTCCACC AGATGGTACG GTGTTGGTCT CAATCAAAAG CTCTTTACCA
    GAAAATAATT TTTTCAACGA TGCTTTGATT GATGAGCTTT TACAGCAGTT TACAAATTTC
    GGTGAAGTTA TACTCATAAG ATTTGTGGAA GATAAAATGT GGGTTACGTT TTTAGAGGGA
    AGCTCTGCCT TGAATGTTCT GAACCTGAAT GGGAAAGAGT TACTGGGTAG GACAATAACA
    ATTACTTTAA AAAGTCCAGA CTGGATCAAA ACTTTGGAAG AAGAAATGAG CTTAGAGAAA
    ATCAACGTCC CCTTGCCATC ATCAACCAGC TCCACCCTCC TAGGTGAAGA CGCGGAGGTC
    ACGGCCGACT TCGATATGGA AGGTGATGTG GATGACTATA GTGCTGAGGT AGAGGAGATC
    CTTCCTCAGC ATCTCCAGCC GTCTTCCAGT TCTGGCCTTG GCACGTCCCC CGGTTCTTCA
    CCCCGGACCA GTCCCTGCCA GTCACCTACC ATATCGGAGG GGCCTGTGCC TTCCCTCCCA
    GTGAGACCAA GTCGAGCTCC GTCCAGAACG CCCGGGCCCC CTGCTTCACA AAGTTCTCCT
    GTTGACACTC TGCCGGCAAC ACAGCTGCAG CAGAAAGATT CTTCCCAGAC CCTGGAACCC
    AAGCGGCCTC CCCCTCCCCG CCCGGTTGCT CCTCCTGCAC GTCCAGCTCC TCCACAACGA
    CCACCTCCGC CTTCAGGACG CAGCCAGCTT CCACCTCAAG CCGGACTGCC AGGCCCGGGA
    CTTGCTGGAC ACAGTGCAGC CAGACCGATT ATTCCGCCCC GTGCTGGAGT CATCAGCGCC
    CCCCAGAGCC ACGCACGGGT GTCTGCTGGG AGACTGACTC CTGAAAGCCA AAGCAAAACC
    TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT
    CCTGTGCCGC CTTCTCTTGC GCCACCTTCT CTTGCGCCAC CTGTTCAGAA GATGCAGGAG
    CCTCTCATCC CGGTGGCCGC ACCTCTGGCC CAGGCTGCCC TGCAGCCCAG CCTGGAAACG
    CCCCCGCAGC CACCCCCTCG GAGCAGGTCG TCCCACAGCT TGCCTTCTGA GCCTCCGGCG
    CAGCCGCAGG TGAAAACAAA CGGAGTCTCC GCCGTCAGAC TGGACTCGCC ATTAAAGAGT
    GACCCATTTG AAGACTTGTC ATTGAACCTG CTTGCTGTAT CAAAGGCTCA GCCATCTGTT
    CACACCCCAA ACCCAAGGGG GTTGACGCCG TTGCCTTCTG CAGCCCCAAG TAACACCAAC
    ACTCTGAGTT CTGTAAGCTG CATGCCGACA ATGCCTCCAA TTCCAGCCCG GAGTAAATCC
    CAGGAAAACA CGCGATGTTC CCCAAACCCA TTCATCCCAA GCTCAAGCAG CACAAATCCT
    TTCACCGACA GGACCGCCGC TCCTGGAAAC CCCTTTCGAG CTGAGTCTCA AGAATCAGAG
    GCCACTTCAT GGTTCTCCAA AGAAGAGCCT GTTGCTCCGA GTCCATTCTC TTCGCTGAGG
    CCTCTGGATC AGAACAGTAG CAAGCCTTCA TCCTCCCTGG ATGGGTTTAA GGACAGTTTT
    GATCCACAGG GCCTGTCTGC ACTAACAGTC AGCAACCCCA AAGGATGGGT AACCTTCGAG
    GAAGAAGAGG ACTTTGGTGT GACAGGGAAG TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG
    GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC AAGGTGATGC TTGGTGATGA CTGGGGTAGG
    AGTACCAATG TGTCTCTCTG TGTGTTGCCA GCAAGACCAC CACCCCCACC GCCTGTCCCT
    CTGCTCCCAC CTGGCACCAC CCCTCCCGCA GACGCTTTCA CAGCCCTGGC CTCTAAGGCA
    TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87627
    Clone ID Related Accession (Same CDS sequence) XM_015463989.1
    Accession Version XM_015463989.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTACATT ATCTGGTCCT AGTCACTGGA TGTATGTCTG TTGGAAAAAT TCAAGAATCT 
    GAAGTTTTCC GAGTTACTTC CACTGAATTT ATATCACTGC GAGTTGATTC TTCGGATGAG
    GATCGCATTT CGGAAGTGCG AAAAGTTTTA AATTCAGGAA ACTTTTATTT TGCATGGTCT
    GCATCCGGAG TCAGTTTGGA TCTGAGTCTT AATGCCCACC GTAGCTTGCA AGAACACACA
    ACTGACAATA GATTTTTCTG GAATCAGTCT TTGCACTTGC ATCTCAAGCA CTACGGTGTG
    AATTGTGACG ACTGGTTATT ACGCCTCATG TGTGGGGGAG TAGAAATCAG AACAATTTAT
    GCTGCTCATA AACAGGCAAA GGCTTGCCTC ATTTCCAGAC TAAGCTGTGA ACGAGCTGGG
    ACCAGGTTTA ACGTCCGGGG AACAAATGAT GATGGTCACG TTGCCAATTT TGTAGAAACA
    GAACAGGTTG TGTACTTAGA TGACTCTGTT TCTTCCTTCA TACAAATCCG GGGATCTGTC
    CCATTGTTCT GGGAGCAGCC AGGATTGCAG GTGGGATCTC ATCGTGTCCG TATGTCAAGG
    GGATTTGAAG CCAATGCACC TGCTTTTGAC AGGCATTTTA GGACACTTAA GAATTTGTAT
    GGTAAACAAA TAATAGTAAA TCTGCTTGGA TCTAAGGAAG GTGAACATAT GCTAAGTAAG
    GCTTTCCAGA GTCATCTGAA AGCTTCTGAA CATGCTGCTG ATATCCAGAT GGTGAATTTT
    GACTATCATC AAATGGTTAA AGGAGGAAAG GCAGAAAAAT TACATAGTGT TCTTAAACCT
    CAAGTCCAGA AGTTTCTGGA TTATGGAATT TTTCATTTTG ATGGAAGTGA AGTTCAAAGG
    TGCCAGAGTG GTACAGTTCG AACAAACTGC TTGGATTGTC TTGACAGAAC AAATAGTGTG
    CAGGCATTCC TTGGTTTAGA GATGCTGACT AAACAGTTGG AAGCTCTTGG TTTAGCTGAA
    AAGCCTCAGT TGGTGACTCG CTTTCAAGAA GTTTTTCGAT CAATGTGGTC TGTGAATGGT
    GATTCAATCA GTAAGATTTA TGCAGGAACT GGAGCCCTTG AAGGAAAGGC TAAGGCTGGA
    AAGTTAAAAG ATGGTGCTCG TTCAGTTAGT AGAACAATTC AGAATAACTT CTTTGACAGC
    TCCAAGCAAG AAGCAATTGA TGTTTTGCTC CTGGGAAATA CTCTAAATAG TGATTTAGCT
    GACAAAGCTC GAGCCCTTTT AACTACTGGA AGTTTGCGTG TTTCTGAACA GACGTTACAG
    TCAGCATCTT CTAAAGTACT AAAGAGTATG TGTGAGAATT TCTACAAGTA TTCAAAGCCC
    AAGAAAATTC GAGTATGTGT GGGCACATGG AACGTGAATG GCGGGAAGCA GTTTCGCAGC
    ATAGCTTTTA AGAATCAGAC CCTTACGGAC TGGCTTCTTG ATGCCCCCAA GTTAGCTGGC
    ATCCAGGAGT TTCAAGATAA AAGAAGTAAA CCAATGGATA TATTTGCAAT TGGTTTTGAG
    GAAATGGTAG AGCTGAATGC TGGAAACATT GTGAATGCAA GCACAACAAA TCAGAAGCTC
    TGGGCTGCGG AACTTCAGAA GACCATCTCC AGAGACAACA AGTATGTGCT GCTGGCCTCT
    GAGCAGTTGG TGGGCGTCTG TCTGTTTGTT TTTATCAGAC CACAGCACGC TCCCTTCATC
    AGGGATGTTG CAGTTGATAC TGTGAAAACT GGAATGGGAG GCGCAACTGG AAATAAGGGA
    GCAGTTGCAA TACGAATGCT GTTCCACACC ACAAGCCTCT GCTTTGTCTG CAGCCACTTC
    GCTGCGGGAC AATCCCAAGT CAAAGAACGA AATGATGATT TTGTAGAAAT AGCGCGGAAG
    TTGAGTTTTC CAATGGGAAG GCTGCTCTTC TCCCATGACT ATGTGTTTTG GTGTGGCGAT
    TTCAACTACC GAATCGATCT CCCTAATGAG GAAGTGAAAG AGCTTATCAG ACAGCAAAAC
    TGGGATTCTC TTATCGCAGG AGATCAGCTT ATCAATCAGA AAAATGCTGG ACAGATTTTT
    AGAGGATTTT TAGAAGGAAA AGTGACCTTT GCTCCAACGT ATAAATACGA CTTGTTTTCT
    GATGACTACG ACACTAGTGA AAAGTGCCGC ACCCCTGCAT GGACAGACCG TGTCCTCTGG
    AGAAGACGGA AGTGGCCTTT TGATAGATCA GCTGAAGATT TAGATCTCCT AAATGCTAGT
    TTTCAAGATG AAAGCAAAAT CCTCTACACA TGGACTCCTG GCACTTTGCT GCACTACGGA
    AGGGCTGAGC TGAAGACTTC TGACCATAGG CCTGTTGTTG CCCTGATCGA TATTGATATA
    TTTGAAGTTG AAGCTGAAGA GAGGCAAAAC ATTTATAAAG AAGTAATTGC AGTTCAGGGT
    CCACCAGATG GTACGGTGTT GGTCTCAATC AAAAGCTCTT TACCAGAAAA TAATTTTTTC
    AACGATGCTT TGATTGATGA GCTTTTACAG CAGTTTACAA ATTTCGGTGA AGTTATACTC
    ATAAGATTTG TGGAAGATAA AATGTGGGTT ACGTTTTTAG AGGGAAGCTC TGCCTTGAAT
    GTTCTGAACC TGAATGGGAA AGAGTTACTG GGTAGGACAA TAACAATTAC TTTAAAAAGT
    CCAGACTGGA TCAAAACTTT GGAAGAAGAA ATGAGCTTAG AGAAAATCAA CGTCCCCTTG
    CCATCATCAA CCAGCTCCAC CCTCCTAGGT GAAGACGCGG AGGTCACGGC CGACTTCGAT
    ATGGAAGGTG ATGTGGATGA CTATAGTGCT GAGGTAGAGG AGATCCTTCC TCAGCATCTC
    CAGCCGTCTT CCAGTTCTGG CCTTGGCACG TCCCCCGGTT CTTCACCCCG GACCAGTCCC
    TGCCAGTCAC CTACCATATC GGAGGGGCCT GTGCCTTCCC TCCCAGTGAG ACCAAGTCGA
    GCTCCGTCCA GAACGCCCGG GCCCCCTGCT TCACAAAGTT CTCCTGTTGA CACTCTGCCG
    GCAACACAGC TGCAGCAGAA AGATTCTTCC CAGACCCTGG AACCCAAGCG GCCTCCCCCT
    CCCCGCCCGG TTGCTCCTCC TGCACGTCCA GCTCCTCCAC AACGACCACC TCCGCCTTCA
    GGGGCTAGGA GTCCTGCACC TGCTAGAAAA GAGTTTGGAG GTGTTGGAGC CCCTCCCAGT
    CCGGGGGTAG CTAGGCGAGA GATGGAAGCA CCCAAAAGCC CAGGAACGAC CCGGAGAGAT
    AATCTAGGAC GCAGCCAGCT TCCACCTCAA GCCGGACTGC CAGGCCCGGG ACTTGCTGGA
    CACAGTGCAG CCAGACCGAT TATTCCGCCC CGTGCTGGAG TCATCAGCGC CCCCCAGAGC
    CACGCACGGG TGTCTGCTGG GAGACTGACT CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA
    CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG
    CCTTCTCTTG CGCCACCTTC TCTTGCGCCA CCTGTTCAGA AGATGCAGGA GCCTCTCATC
    CCGGTGGCCG CACCTCTGGC CCAGGCTGCC CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG
    CCACCCCCTC GGAGCAGGTC GTCCCACAGC TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG
    GTGAAAACAA ACGGAGTCTC CGCCGTCAGA CTGGACTCGC CATTAAAGAG TGACCCATTT
    GAAGACTTGT CATTGAACCT GCTTGCTGTA TCAAAGGCTC AGCCATCTGT TCACACCCCA
    AACCCAAGGG GGTTGACGCC GTTGCCTTCT GCAGCCCCAA GTAACACCAA CACTCTGAGT
    TCTGTAAGCT GCATGCCGAC AATGCCTCCA ATTCCAGCCC GGAGTAAATC CCAGGAAAAC
    ACGCGATGTT CCCCAAACCC ATTCATCCCA AGCTCAAGCA GCACAAATCC TTTCACCGAC
    AGGACCGCCG CTCCTGGAAA CCCCTTTCGA GCTGAGTCTC AAGAATCAGA GGCCACTTCA
    TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG AGTCCATTCT CTTCGCTGAG GCCTCTGGAT
    CAGAACAGTA GCAAGCCTTC ATCCTCCCTG GATGGGTTTA AGGACAGTTT TGATCCACAG
    GGCCTGTCTG CACTAACAGT CAGCAACCCC AAAGGATGGG TAACCTTCGA GGAAGAAGAG
    GACTTTGGTG TGACAGGGAA GTCAGGGTCC ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG
    CTGAGCTCGT CTCCTGGCTC CAAGGTGATG CTTGGTGATG ACTGGGGTAG GAGTACCAAT
    GTGTCTCTCT GTGTGTTGCC AGCAAGACCA CCACCCCCAC CGCCTGTCCC TCTGCTCCCA
    CCTGGCACCA CCCCTCCCGC AGACGCTTTC ACAGCCCTGG CCTCTAAGGC ATCACCCACG
    CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319475.1
    CDS270..4790
    Translation

    Target ORF information:

    RefSeq Version XM_015463989.1
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTACATT ATCTGGTCCT AGTCACTGGA TGTATGTCTG TTGGAAAAAT TCAAGAATCT 
    GAAGTTTTCC GAGTTACTTC CACTGAATTT ATATCACTGC GAGTTGATTC TTCGGATGAG
    GATCGCATTT CGGAAGTGCG AAAAGTTTTA AATTCAGGAA ACTTTTATTT TGCATGGTCT
    GCATCCGGAG TCAGTTTGGA TCTGAGTCTT AATGCCCACC GTAGCTTGCA AGAACACACA
    ACTGACAATA GATTTTTCTG GAATCAGTCT TTGCACTTGC ATCTCAAGCA CTACGGTGTG
    AATTGTGACG ACTGGTTATT ACGCCTCATG TGTGGGGGAG TAGAAATCAG AACAATTTAT
    GCTGCTCATA AACAGGCAAA GGCTTGCCTC ATTTCCAGAC TAAGCTGTGA ACGAGCTGGG
    ACCAGGTTTA ACGTCCGGGG AACAAATGAT GATGGTCACG TTGCCAATTT TGTAGAAACA
    GAACAGGTTG TGTACTTAGA TGACTCTGTT TCTTCCTTCA TACAAATCCG GGGATCTGTC
    CCATTGTTCT GGGAGCAGCC AGGATTGCAG GTGGGATCTC ATCGTGTCCG TATGTCAAGG
    GGATTTGAAG CCAATGCACC TGCTTTTGAC AGGCATTTTA GGACACTTAA GAATTTGTAT
    GGTAAACAAA TAATAGTAAA TCTGCTTGGA TCTAAGGAAG GTGAACATAT GCTAAGTAAG
    GCTTTCCAGA GTCATCTGAA AGCTTCTGAA CATGCTGCTG ATATCCAGAT GGTGAATTTT
    GACTATCATC AAATGGTTAA AGGAGGAAAG GCAGAAAAAT TACATAGTGT TCTTAAACCT
    CAAGTCCAGA AGTTTCTGGA TTATGGAATT TTTCATTTTG ATGGAAGTGA AGTTCAAAGG
    TGCCAGAGTG GTACAGTTCG AACAAACTGC TTGGATTGTC TTGACAGAAC AAATAGTGTG
    CAGGCATTCC TTGGTTTAGA GATGCTGACT AAACAGTTGG AAGCTCTTGG TTTAGCTGAA
    AAGCCTCAGT TGGTGACTCG CTTTCAAGAA GTTTTTCGAT CAATGTGGTC TGTGAATGGT
    GATTCAATCA GTAAGATTTA TGCAGGAACT GGAGCCCTTG AAGGAAAGGC TAAGGCTGGA
    AAGTTAAAAG ATGGTGCTCG TTCAGTTAGT AGAACAATTC AGAATAACTT CTTTGACAGC
    TCCAAGCAAG AAGCAATTGA TGTTTTGCTC CTGGGAAATA CTCTAAATAG TGATTTAGCT
    GACAAAGCTC GAGCCCTTTT AACTACTGGA AGTTTGCGTG TTTCTGAACA GACGTTACAG
    TCAGCATCTT CTAAAGTACT AAAGAGTATG TGTGAGAATT TCTACAAGTA TTCAAAGCCC
    AAGAAAATTC GAGTATGTGT GGGCACATGG AACGTGAATG GCGGGAAGCA GTTTCGCAGC
    ATAGCTTTTA AGAATCAGAC CCTTACGGAC TGGCTTCTTG ATGCCCCCAA GTTAGCTGGC
    ATCCAGGAGT TTCAAGATAA AAGAAGTAAA CCAATGGATA TATTTGCAAT TGGTTTTGAG
    GAAATGGTAG AGCTGAATGC TGGAAACATT GTGAATGCAA GCACAACAAA TCAGAAGCTC
    TGGGCTGCGG AACTTCAGAA GACCATCTCC AGAGACAACA AGTATGTGCT GCTGGCCTCT
    GAGCAGTTGG TGGGCGTCTG TCTGTTTGTT TTTATCAGAC CACAGCACGC TCCCTTCATC
    AGGGATGTTG CAGTTGATAC TGTGAAAACT GGAATGGGAG GCGCAACTGG AAATAAGGGA
    GCAGTTGCAA TACGAATGCT GTTCCACACC ACAAGCCTCT GCTTTGTCTG CAGCCACTTC
    GCTGCGGGAC AATCCCAAGT CAAAGAACGA AATGATGATT TTGTAGAAAT AGCGCGGAAG
    TTGAGTTTTC CAATGGGAAG GCTGCTCTTC TCCCATGACT ATGTGTTTTG GTGTGGCGAT
    TTCAACTACC GAATCGATCT CCCTAATGAG GAAGTGAAAG AGCTTATCAG ACAGCAAAAC
    TGGGATTCTC TTATCGCAGG AGATCAGCTT ATCAATCAGA AAAATGCTGG ACAGATTTTT
    AGAGGATTTT TAGAAGGAAA AGTGACCTTT GCTCCAACGT ATAAATACGA CTTGTTTTCT
    GATGACTACG ACACTAGTGA AAAGTGCCGC ACCCCTGCAT GGACAGACCG TGTCCTCTGG
    AGAAGACGGA AGTGGCCTTT TGATAGATCA GCTGAAGATT TAGATCTCCT AAATGCTAGT
    TTTCAAGATG AAAGCAAAAT CCTCTACACA TGGACTCCTG GCACTTTGCT GCACTACGGA
    AGGGCTGAGC TGAAGACTTC TGACCATAGG CCTGTTGTTG CCCTGATCGA TATTGATATA
    TTTGAAGTTG AAGCTGAAGA GAGGCAAAAC ATTTATAAAG AAGTAATTGC AGTTCAGGGT
    CCACCAGATG GTACGGTGTT GGTCTCAATC AAAAGCTCTT TACCAGAAAA TAATTTTTTC
    AACGATGCTT TGATTGATGA GCTTTTACAG CAGTTTACAA ATTTCGGTGA AGTTATACTC
    ATAAGATTTG TGGAAGATAA AATGTGGGTT ACGTTTTTAG AGGGAAGCTC TGCCTTGAAT
    GTTCTGAACC TGAATGGGAA AGAGTTACTG GGTAGGACAA TAACAATTAC TTTAAAAAGT
    CCAGACTGGA TCAAAACTTT GGAAGAAGAA ATGAGCTTAG AGAAAATCAA CGTCCCCTTG
    CCATCATCAA CCAGCTCCAC CCTCCTAGGT GAAGACGCGG AGGTCACGGC CGACTTCGAT
    ATGGAAGGTG ATGTGGATGA CTATAGTGCT GAGGTAGAGG AGATCCTTCC TCAGCATCTC
    CAGCCGTCTT CCAGTTCTGG CCTTGGCACG TCCCCCGGTT CTTCACCCCG GACCAGTCCC
    TGCCAGTCAC CTACCATATC GGAGGGGCCT GTGCCTTCCC TCCCAGTGAG ACCAAGTCGA
    GCTCCGTCCA GAACGCCCGG GCCCCCTGCT TCACAAAGTT CTCCTGTTGA CACTCTGCCG
    GCAACACAGC TGCAGCAGAA AGATTCTTCC CAGACCCTGG AACCCAAGCG GCCTCCCCCT
    CCCCGCCCGG TTGCTCCTCC TGCACGTCCA GCTCCTCCAC AACGACCACC TCCGCCTTCA
    GGGGCTAGGA GTCCTGCACC TGCTAGAAAA GAGTTTGGAG GTGTTGGAGC CCCTCCCAGT
    CCGGGGGTAG CTAGGCGAGA GATGGAAGCA CCCAAAAGCC CAGGAACGAC CCGGAGAGAT
    AATCTAGGAC GCAGCCAGCT TCCACCTCAA GCCGGACTGC CAGGCCCGGG ACTTGCTGGA
    CACAGTGCAG CCAGACCGAT TATTCCGCCC CGTGCTGGAG TCATCAGCGC CCCCCAGAGC
    CACGCACGGG TGTCTGCTGG GAGACTGACT CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA
    CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG
    CCTTCTCTTG CGCCACCTTC TCTTGCGCCA CCTGTTCAGA AGATGCAGGA GCCTCTCATC
    CCGGTGGCCG CACCTCTGGC CCAGGCTGCC CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG
    CCACCCCCTC GGAGCAGGTC GTCCCACAGC TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG
    GTGAAAACAA ACGGAGTCTC CGCCGTCAGA CTGGACTCGC CATTAAAGAG TGACCCATTT
    GAAGACTTGT CATTGAACCT GCTTGCTGTA TCAAAGGCTC AGCCATCTGT TCACACCCCA
    AACCCAAGGG GGTTGACGCC GTTGCCTTCT GCAGCCCCAA GTAACACCAA CACTCTGAGT
    TCTGTAAGCT GCATGCCGAC AATGCCTCCA ATTCCAGCCC GGAGTAAATC CCAGGAAAAC
    ACGCGATGTT CCCCAAACCC ATTCATCCCA AGCTCAAGCA GCACAAATCC TTTCACCGAC
    AGGACCGCCG CTCCTGGAAA CCCCTTTCGA GCTGAGTCTC AAGAATCAGA GGCCACTTCA
    TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG AGTCCATTCT CTTCGCTGAG GCCTCTGGAT
    CAGAACAGTA GCAAGCCTTC ATCCTCCCTG GATGGGTTTA AGGACAGTTT TGATCCACAG
    GGCCTGTCTG CACTAACAGT CAGCAACCCC AAAGGATGGG TAACCTTCGA GGAAGAAGAG
    GACTTTGGTG TGACAGGGAA GTCAGGGTCC ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG
    CTGAGCTCGT CTCCTGGCTC CAAGGTGATG CTTGGTGATG ACTGGGGTAG GAGTACCAAT
    GTGTCTCTCT GTGTGTTGCC AGCAAGACCA CCACCCCCAC CGCCTGTCCC TCTGCTCCCA
    CCTGGCACCA CCCCTCCCGC AGACGCTTTC ACAGCCCTGG CCTCTAAGGC ATCACCCACG
    CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45325
    Clone ID Related Accession (Same CDS sequence) XM_005201086.3 , XM_005201086.4
    Accession Version XM_005201086.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201086.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGGTG AAAACAAACG GAGTCTCCGC CGTCAGACTG
    GACTCGCCAT TAAAGAGTGA CCCATTTGAA GACTTGTCAT TGAACCTGCT TGCTGTATCA
    AAGGCTCAGC CATCTGTTCA CACCCCAAAC CCAAGGGGGT TGACGCCGTT GCCTTCTGCA
    GCCCCAAGTA ACACCAACAC TCTGAGTTCT GTAAGCTGCA TGCCGACAAT GCCTCCAATT
    CCAGCCCGGA GTAAATCCCA GGAAAACACG CGATGTTCCC CAAACCCATT CATCCCAAGC
    TCAAGCAGCA CAAATCCTTT CACCGACAGG ACCGCCGCTC CTGGAAACCC CTTTCGAGCT
    GAGTCTCAAG AATCAGAGGC CACTTCATGG TTCTCCAAAG AAGAGCCTGT TGCTCCGAGT
    CCATTCTCTT CGCTGAGGCC TCTGGATCAG AACAGTAGCA AGCCTTCATC CTCCCTGGAT
    GGGTTTAAGG ACAGTTTTGA TCCACAGGGC CTGTCTGCAC TAACAGTCAG CAACCCCAAA
    GGATGGGTAA CCTTCGAGGA AGAAGAGGAC TTTGGTGTGA CAGGGAAGTC AGGGTCCACT
    CGCCCAGACG TTTTCCTGGG TAAGCAGCTG AGCTCGTCTC CTGGCTCCAA GGTGATGCTT
    GGTGATGACT GGGGTAGGAG TACCAATGTG TCTCTCTGTG TGTTGCCAGC AAGACCACCA
    CCCCCACCGC CTGTCCCTCT GCTCCCACCT GGCACCACCC CTCCCGCAGA CGCTTTCACA
    GCCCTGGCCT CTAAGGCATC ACCCACGCTA GACTTGACAG AAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201143.2
    CDS29..4876
    Translation

    Target ORF information:

    RefSeq Version XM_005201086.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201086.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGGTG AAAACAAACG GAGTCTCCGC CGTCAGACTG
    GACTCGCCAT TAAAGAGTGA CCCATTTGAA GACTTGTCAT TGAACCTGCT TGCTGTATCA
    AAGGCTCAGC CATCTGTTCA CACCCCAAAC CCAAGGGGGT TGACGCCGTT GCCTTCTGCA
    GCCCCAAGTA ACACCAACAC TCTGAGTTCT GTAAGCTGCA TGCCGACAAT GCCTCCAATT
    CCAGCCCGGA GTAAATCCCA GGAAAACACG CGATGTTCCC CAAACCCATT CATCCCAAGC
    TCAAGCAGCA CAAATCCTTT CACCGACAGG ACCGCCGCTC CTGGAAACCC CTTTCGAGCT
    GAGTCTCAAG AATCAGAGGC CACTTCATGG TTCTCCAAAG AAGAGCCTGT TGCTCCGAGT
    CCATTCTCTT CGCTGAGGCC TCTGGATCAG AACAGTAGCA AGCCTTCATC CTCCCTGGAT
    GGGTTTAAGG ACAGTTTTGA TCCACAGGGC CTGTCTGCAC TAACAGTCAG CAACCCCAAA
    GGATGGGTAA CCTTCGAGGA AGAAGAGGAC TTTGGTGTGA CAGGGAAGTC AGGGTCCACT
    CGCCCAGACG TTTTCCTGGG TAAGCAGCTG AGCTCGTCTC CTGGCTCCAA GGTGATGCTT
    GGTGATGACT GGGGTAGGAG TACCAATGTG TCTCTCTGTG TGTTGCCAGC AAGACCACCA
    CCCCCACCGC CTGTCCCTCT GCTCCCACCT GGCACCACCC CTCCCGCAGA CGCTTTCACA
    GCCCTGGCCT CTAAGGCATC ACCCACGCTA GACTTGACAG AAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45325
    Clone ID Related Accession (Same CDS sequence) XM_005201086.3 , XM_005201086.4
    Accession Version XM_005201086.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201086.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGGTG AAAACAAACG GAGTCTCCGC CGTCAGACTG
    GACTCGCCAT TAAAGAGTGA CCCATTTGAA GACTTGTCAT TGAACCTGCT TGCTGTATCA
    AAGGCTCAGC CATCTGTTCA CACCCCAAAC CCAAGGGGGT TGACGCCGTT GCCTTCTGCA
    GCCCCAAGTA ACACCAACAC TCTGAGTTCT GTAAGCTGCA TGCCGACAAT GCCTCCAATT
    CCAGCCCGGA GTAAATCCCA GGAAAACACG CGATGTTCCC CAAACCCATT CATCCCAAGC
    TCAAGCAGCA CAAATCCTTT CACCGACAGG ACCGCCGCTC CTGGAAACCC CTTTCGAGCT
    GAGTCTCAAG AATCAGAGGC CACTTCATGG TTCTCCAAAG AAGAGCCTGT TGCTCCGAGT
    CCATTCTCTT CGCTGAGGCC TCTGGATCAG AACAGTAGCA AGCCTTCATC CTCCCTGGAT
    GGGTTTAAGG ACAGTTTTGA TCCACAGGGC CTGTCTGCAC TAACAGTCAG CAACCCCAAA
    GGATGGGTAA CCTTCGAGGA AGAAGAGGAC TTTGGTGTGA CAGGGAAGTC AGGGTCCACT
    CGCCCAGACG TTTTCCTGGG TAAGCAGCTG AGCTCGTCTC CTGGCTCCAA GGTGATGCTT
    GGTGATGACT GGGGTAGGAG TACCAATGTG TCTCTCTGTG TGTTGCCAGC AAGACCACCA
    CCCCCACCGC CTGTCCCTCT GCTCCCACCT GGCACCACCC CTCCCGCAGA CGCTTTCACA
    GCCCTGGCCT CTAAGGCATC ACCCACGCTA GACTTGACAG AAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201143.2
    CDS16..4863
    Translation

    Target ORF information:

    RefSeq Version XM_005201086.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201086.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGGTG AAAACAAACG GAGTCTCCGC CGTCAGACTG
    GACTCGCCAT TAAAGAGTGA CCCATTTGAA GACTTGTCAT TGAACCTGCT TGCTGTATCA
    AAGGCTCAGC CATCTGTTCA CACCCCAAAC CCAAGGGGGT TGACGCCGTT GCCTTCTGCA
    GCCCCAAGTA ACACCAACAC TCTGAGTTCT GTAAGCTGCA TGCCGACAAT GCCTCCAATT
    CCAGCCCGGA GTAAATCCCA GGAAAACACG CGATGTTCCC CAAACCCATT CATCCCAAGC
    TCAAGCAGCA CAAATCCTTT CACCGACAGG ACCGCCGCTC CTGGAAACCC CTTTCGAGCT
    GAGTCTCAAG AATCAGAGGC CACTTCATGG TTCTCCAAAG AAGAGCCTGT TGCTCCGAGT
    CCATTCTCTT CGCTGAGGCC TCTGGATCAG AACAGTAGCA AGCCTTCATC CTCCCTGGAT
    GGGTTTAAGG ACAGTTTTGA TCCACAGGGC CTGTCTGCAC TAACAGTCAG CAACCCCAAA
    GGATGGGTAA CCTTCGAGGA AGAAGAGGAC TTTGGTGTGA CAGGGAAGTC AGGGTCCACT
    CGCCCAGACG TTTTCCTGGG TAAGCAGCTG AGCTCGTCTC CTGGCTCCAA GGTGATGCTT
    GGTGATGACT GGGGTAGGAG TACCAATGTG TCTCTCTGTG TGTTGCCAGC AAGACCACCA
    CCCCCACCGC CTGTCCCTCT GCTCCCACCT GGCACCACCC CTCCCGCAGA CGCTTTCACA
    GCCCTGGCCT CTAAGGCATC ACCCACGCTA GACTTGACAG AAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45326
    Clone ID Related Accession (Same CDS sequence) XM_005201087.3
    Accession Version XM_005201087.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GTTAAAAGAT GGTGCTCGTT CAGTTAGTAG AACAATTCAG
    AATAACTTCT TTGACAGCTC CAAGCAAGAA GCAATTGATG TTTTGCTCCT GGGAAATACT
    CTAAATAGTG ATTTAGCTGA CAAAGCTCGA GCCCTTTTAA CTACTGGAAG TTTGCGTGTT
    TCTGAACAGA CGTTACAGTC AGCATCTTCT AAAGTACTAA AGAGTATGTG TGAGAATTTC
    TACAAGTATT CAAAGCCCAA GAAAATTCGA GTATGTGTGG GCACATGGAA CGTGAATGGC
    GGGAAGCAGT TTCGCAGCAT AGCTTTTAAG AATCAGACCC TTACGGACTG GCTTCTTGAT
    GCCCCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA GAAGTAAACC AATGGATATA
    TTTGCAATTG GTTTTGAGGA AATGGTAGAG CTGAATGCTG GAAACATTGT GAATGCAAGC
    ACAACAAATC AGAAGCTCTG GGCTGCGGAA CTTCAGAAGA CCATCTCCAG AGACAACAAG
    TATGTGCTGC TGGCCTCTGA GCAGTTGGTG GGCGTCTGTC TGTTTGTTTT TATCAGACCA
    CAGCACGCTC CCTTCATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG AATGGGAGGC
    GCAACTGGAA ATAAGGGAGC AGTTGCAATA CGAATGCTGT TCCACACCAC AAGCCTCTGC
    TTTGTCTGCA GCCACTTCGC TGCGGGACAA TCCCAAGTCA AAGAACGAAA TGATGATTTT
    GTAGAAATAG CGCGGAAGTT GAGTTTTCCA ATGGGAAGGC TGCTCTTCTC CCATGACTAT
    GTGTTTTGGT GTGGCGATTT CAACTACCGA ATCGATCTCC CTAATGAGGA AGTGAAAGAG
    CTTATCAGAC AGCAAAACTG GGATTCTCTT ATCGCAGGAG ATCAGCTTAT CAATCAGAAA
    AATGCTGGAC AGATTTTTAG AGGATTTTTA GAAGGAAAAG TGACCTTTGC TCCAACGTAT
    AAATACGACT TGTTTTCTGA TGACTACGAC ACTAGTGAAA AGTGCCGCAC CCCTGCATGG
    ACAGACCGTG TCCTCTGGAG AAGACGGAAG TGGCCTTTTG ATAGATCAGC TGAAGATTTA
    GATCTCCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATCC TCTACACATG GACTCCTGGC
    ACTTTGCTGC ACTACGGAAG GGCTGAGCTG AAGACTTCTG ACCATAGGCC TGTTGTTGCC
    CTGATCGATA TTGATATATT TGAAGTTGAA GCTGAAGAGA GGCAAAACAT TTATAAAGAA
    GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACGGTGTTGG TCTCAATCAA AAGCTCTTTA
    CCAGAAAATA ATTTTTTCAA CGATGCTTTG ATTGATGAGC TTTTACAGCA GTTTACAAAT
    TTCGGTGAAG TTATACTCAT AAGATTTGTG GAAGATAAAA TGTGGGTTAC GTTTTTAGAG
    GGAAGCTCTG CCTTGAATGT TCTGAACCTG AATGGGAAAG AGTTACTGGG TAGGACAATA
    ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG AAGAAGAAAT GAGCTTAGAG
    AAAATCAACG TCCCCTTGCC ATCATCAACC AGCTCCACCC TCCTAGGTGA AGACGCGGAG
    GTCACGGCCG ACTTCGATAT GGAAGGTGAT GTGGATGACT ATAGTGCTGA GGTAGAGGAG
    ATCCTTCCTC AGCATCTCCA GCCGTCTTCC AGTTCTGGCC TTGGCACGTC CCCCGGTTCT
    TCACCCCGGA CCAGTCCCTG CCAGTCACCT ACCATATCGG AGGGGCCTGT GCCTTCCCTC
    CCAGTGAGAC CAAGTCGAGC TCCGTCCAGA ACGCCCGGGC CCCCTGCTTC ACAAAGTTCT
    CCTGTTGACA CTCTGCCGGC AACACAGCTG CAGCAGAAAG ATTCTTCCCA GACCCTGGAA
    CCCAAGCGGC CTCCCCCTCC CCGCCCGGTT GCTCCTCCTG CACGTCCAGC TCCTCCACAA
    CGACCACCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCTG CTAGAAAAGA GTTTGGAGGT
    GTTGGAGCCC CTCCCAGTCC GGGGGTAGCT AGGCGAGAGA TGGAAGCACC CAAAAGCCCA
    GGAACGACCC GGAGAGATAA TCTAGGACGC AGCCAGCTTC CACCTCAAGC CGGACTGCCA
    GGCCCGGGAC TTGCTGGACA CAGTGCAGCC AGACCGATTA TTCCGCCCCG TGCTGGAGTC
    ATCAGCGCCC CCCAGAGCCA CGCACGGGTG TCTGCTGGGA GACTGACTCC TGAAAGCCAA
    AGCAAAACCT CAGAAGTACT GAAAGGGCCA GCTCTTCTTC CCGAGCCCCT GAAGCCTCAG
    GCCGCCCTTC CTGTGCCGCC TTCTCTTGCG CCACCTTCTC TTGCGCCACC TGTTCAGAAG
    ATGCAGGAGC CTCTCATCCC GGTGGCCGCA CCTCTGGCCC AGGCTGCCCT GCAGCCCAGC
    CTGGAAACGC CCCCGCAGCC ACCCCCTCGG AGCAGGTCGT CCCACAGCTT GCCTTCTGAG
    CCTCCGGCGC AGCCGCAGGT GAAAACAAAC GGAGTCTCCG CCGTCAGACT GGACTCGCCA
    TTAAAGAGTG ACCCATTTGA AGACTTGTCA TTGAACCTGC TTGCTGTATC AAAGGCTCAG
    CCATCTGTTC ACACCCCAAA CCCAAGGGGG TTGACGCCGT TGCCTTCTGC AGCCCCAAGT
    AACACCAACA CTCTGAGTTC TGTAAGCTGC ATGCCGACAA TGCCTCCAAT TCCAGCCCGG
    AGTAAATCCC AGGAAAACAC GCGATGTTCC CCAAACCCAT TCATCCCAAG CTCAAGCAGC
    ACAAATCCTT TCACCGACAG GACCGCCGCT CCTGGAAACC CCTTTCGAGC TGAGTCTCAA
    GAATCAGAGG CCACTTCATG GTTCTCCAAA GAAGAGCCTG TTGCTCCGAG TCCATTCTCT
    TCGCTGAGGC CTCTGGATCA GAACAGTAGC AAGCCTTCAT CCTCCCTGGA TGGGTTTAAG
    GACAGTTTTG ATCCACAGGG CCTGTCTGCA CTAACAGTCA GCAACCCCAA AGGATGGGTA
    ACCTTCGAGG AAGAAGAGGA CTTTGGTGTG ACAGGGAAGT CAGGGTCCAC TCGCCCAGAC
    GTTTTCCTGG GTAAGCAGCT GAGCTCGTCT CCTGGCTCCA AGGTGATGCT TGGTGATGAC
    TGGGGTAGGA GTACCAATGT GTCTCTCTGT GTGTTGCCAG CAAGACCACC ACCCCCACCG
    CCTGTCCCTC TGCTCCCACC TGGCACCACC CCTCCCGCAG ACGCTTTCAC AGCCCTGGCC
    TCTAAGGCAT CACCCACGCT AGACTTGACA GAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201144.2
    CDS29..4867
    Translation

    Target ORF information:

    RefSeq Version XM_005201087.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201087.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GTTAAAAGAT GGTGCTCGTT CAGTTAGTAG AACAATTCAG
    AATAACTTCT TTGACAGCTC CAAGCAAGAA GCAATTGATG TTTTGCTCCT GGGAAATACT
    CTAAATAGTG ATTTAGCTGA CAAAGCTCGA GCCCTTTTAA CTACTGGAAG TTTGCGTGTT
    TCTGAACAGA CGTTACAGTC AGCATCTTCT AAAGTACTAA AGAGTATGTG TGAGAATTTC
    TACAAGTATT CAAAGCCCAA GAAAATTCGA GTATGTGTGG GCACATGGAA CGTGAATGGC
    GGGAAGCAGT TTCGCAGCAT AGCTTTTAAG AATCAGACCC TTACGGACTG GCTTCTTGAT
    GCCCCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA GAAGTAAACC AATGGATATA
    TTTGCAATTG GTTTTGAGGA AATGGTAGAG CTGAATGCTG GAAACATTGT GAATGCAAGC
    ACAACAAATC AGAAGCTCTG GGCTGCGGAA CTTCAGAAGA CCATCTCCAG AGACAACAAG
    TATGTGCTGC TGGCCTCTGA GCAGTTGGTG GGCGTCTGTC TGTTTGTTTT TATCAGACCA
    CAGCACGCTC CCTTCATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG AATGGGAGGC
    GCAACTGGAA ATAAGGGAGC AGTTGCAATA CGAATGCTGT TCCACACCAC AAGCCTCTGC
    TTTGTCTGCA GCCACTTCGC TGCGGGACAA TCCCAAGTCA AAGAACGAAA TGATGATTTT
    GTAGAAATAG CGCGGAAGTT GAGTTTTCCA ATGGGAAGGC TGCTCTTCTC CCATGACTAT
    GTGTTTTGGT GTGGCGATTT CAACTACCGA ATCGATCTCC CTAATGAGGA AGTGAAAGAG
    CTTATCAGAC AGCAAAACTG GGATTCTCTT ATCGCAGGAG ATCAGCTTAT CAATCAGAAA
    AATGCTGGAC AGATTTTTAG AGGATTTTTA GAAGGAAAAG TGACCTTTGC TCCAACGTAT
    AAATACGACT TGTTTTCTGA TGACTACGAC ACTAGTGAAA AGTGCCGCAC CCCTGCATGG
    ACAGACCGTG TCCTCTGGAG AAGACGGAAG TGGCCTTTTG ATAGATCAGC TGAAGATTTA
    GATCTCCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATCC TCTACACATG GACTCCTGGC
    ACTTTGCTGC ACTACGGAAG GGCTGAGCTG AAGACTTCTG ACCATAGGCC TGTTGTTGCC
    CTGATCGATA TTGATATATT TGAAGTTGAA GCTGAAGAGA GGCAAAACAT TTATAAAGAA
    GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACGGTGTTGG TCTCAATCAA AAGCTCTTTA
    CCAGAAAATA ATTTTTTCAA CGATGCTTTG ATTGATGAGC TTTTACAGCA GTTTACAAAT
    TTCGGTGAAG TTATACTCAT AAGATTTGTG GAAGATAAAA TGTGGGTTAC GTTTTTAGAG
    GGAAGCTCTG CCTTGAATGT TCTGAACCTG AATGGGAAAG AGTTACTGGG TAGGACAATA
    ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG AAGAAGAAAT GAGCTTAGAG
    AAAATCAACG TCCCCTTGCC ATCATCAACC AGCTCCACCC TCCTAGGTGA AGACGCGGAG
    GTCACGGCCG ACTTCGATAT GGAAGGTGAT GTGGATGACT ATAGTGCTGA GGTAGAGGAG
    ATCCTTCCTC AGCATCTCCA GCCGTCTTCC AGTTCTGGCC TTGGCACGTC CCCCGGTTCT
    TCACCCCGGA CCAGTCCCTG CCAGTCACCT ACCATATCGG AGGGGCCTGT GCCTTCCCTC
    CCAGTGAGAC CAAGTCGAGC TCCGTCCAGA ACGCCCGGGC CCCCTGCTTC ACAAAGTTCT
    CCTGTTGACA CTCTGCCGGC AACACAGCTG CAGCAGAAAG ATTCTTCCCA GACCCTGGAA
    CCCAAGCGGC CTCCCCCTCC CCGCCCGGTT GCTCCTCCTG CACGTCCAGC TCCTCCACAA
    CGACCACCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCTG CTAGAAAAGA GTTTGGAGGT
    GTTGGAGCCC CTCCCAGTCC GGGGGTAGCT AGGCGAGAGA TGGAAGCACC CAAAAGCCCA
    GGAACGACCC GGAGAGATAA TCTAGGACGC AGCCAGCTTC CACCTCAAGC CGGACTGCCA
    GGCCCGGGAC TTGCTGGACA CAGTGCAGCC AGACCGATTA TTCCGCCCCG TGCTGGAGTC
    ATCAGCGCCC CCCAGAGCCA CGCACGGGTG TCTGCTGGGA GACTGACTCC TGAAAGCCAA
    AGCAAAACCT CAGAAGTACT GAAAGGGCCA GCTCTTCTTC CCGAGCCCCT GAAGCCTCAG
    GCCGCCCTTC CTGTGCCGCC TTCTCTTGCG CCACCTTCTC TTGCGCCACC TGTTCAGAAG
    ATGCAGGAGC CTCTCATCCC GGTGGCCGCA CCTCTGGCCC AGGCTGCCCT GCAGCCCAGC
    CTGGAAACGC CCCCGCAGCC ACCCCCTCGG AGCAGGTCGT CCCACAGCTT GCCTTCTGAG
    CCTCCGGCGC AGCCGCAGGT GAAAACAAAC GGAGTCTCCG CCGTCAGACT GGACTCGCCA
    TTAAAGAGTG ACCCATTTGA AGACTTGTCA TTGAACCTGC TTGCTGTATC AAAGGCTCAG
    CCATCTGTTC ACACCCCAAA CCCAAGGGGG TTGACGCCGT TGCCTTCTGC AGCCCCAAGT
    AACACCAACA CTCTGAGTTC TGTAAGCTGC ATGCCGACAA TGCCTCCAAT TCCAGCCCGG
    AGTAAATCCC AGGAAAACAC GCGATGTTCC CCAAACCCAT TCATCCCAAG CTCAAGCAGC
    ACAAATCCTT TCACCGACAG GACCGCCGCT CCTGGAAACC CCTTTCGAGC TGAGTCTCAA
    GAATCAGAGG CCACTTCATG GTTCTCCAAA GAAGAGCCTG TTGCTCCGAG TCCATTCTCT
    TCGCTGAGGC CTCTGGATCA GAACAGTAGC AAGCCTTCAT CCTCCCTGGA TGGGTTTAAG
    GACAGTTTTG ATCCACAGGG CCTGTCTGCA CTAACAGTCA GCAACCCCAA AGGATGGGTA
    ACCTTCGAGG AAGAAGAGGA CTTTGGTGTG ACAGGGAAGT CAGGGTCCAC TCGCCCAGAC
    GTTTTCCTGG GTAAGCAGCT GAGCTCGTCT CCTGGCTCCA AGGTGATGCT TGGTGATGAC
    TGGGGTAGGA GTACCAATGT GTCTCTCTGT GTGTTGCCAG CAAGACCACC ACCCCCACCG
    CCTGTCCCTC TGCTCCCACC TGGCACCACC CCTCCCGCAG ACGCTTTCAC AGCCCTGGCC
    TCTAAGGCAT CACCCACGCT AGACTTGACA GAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45327
    Clone ID Related Accession (Same CDS sequence) XM_005201088.3 , XM_005201088.4
    Accession Version XM_005201088.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGGGCTA GGAGTCCTGC ACCTGCTAGA AAAGAGTTTG GAGGTGTTGG AGCCCCTCCC
    AGTCCGGGGG TAGCTAGGCG AGAGATGGAA GCACCCAAAA GCCCAGGAAC GACCCGGAGA
    GATAATCTAG GACGCAGCCA GCTTCCACCT CAAGCCGGAC TGCCAGGCCC GGGACTTGCT
    GGACACAGTG CAGCCAGACC GATTATTCCG CCCCGTGCTG GAGTCATCAG CGCCCCCCAG
    AGCCACGCAC GGGTGTCTGC TGGGAGACTG ACTCCTGAAA GCCAAAGCAA AACCTCAGAA
    GTACTGAAAG GGCCAGCTCT TCTTCCCGAG CCCCTGAAGC CTCAGGCCGC CCTTCCTGTG
    CCGCCTTCTC TTGCGCCACC TTCTCTTGCG CCACCTGTTC AGAAGATGCA GGAGCCTCTC
    ATCCCGGTGG CCGCACCTCT GGCCCAGGCT GCCCTGCAGC CCAGCCTGGA AACGCCCCCG
    CAGCCACCCC CTCGGAGCAG GTCGTCCCAC AGCTTGCCTT CTGAGCCTCC GGCGCAGCCG
    CAGGTGAAAA CAAACGGAGT CTCCGCCGTC AGACTGGACT CGCCATTAAA GAGTGACCCA
    TTTGAAGACT TGTCATTGAA CCTGCTTGCT GTATCAAAGG CTCAGCCATC TGTTCACACC
    CCAAACCCAA GGGGGTTGAC GCCGTTGCCT TCTGCAGCCC CAAGTAACAC CAACACTCTG
    AGTTCTGTAA GCTGCATGCC GACAATGCCT CCAATTCCAG CCCGGAGTAA ATCCCAGGAA
    AACACGCGAT GTTCCCCAAA CCCATTCATC CCAAGCTCAA GCAGCACAAA TCCTTTCACC
    GACAGGACCG CCGCTCCTGG AAACCCCTTT CGAGCTGAGT CTCAAGAATC AGAGGCCACT
    TCATGGTTCT CCAAAGAAGA GCCTGTTGCT CCGAGTCCAT TCTCTTCGCT GAGGCCTCTG
    GATCAGAACA GTAGCAAGCC TTCATCCTCC CTGGATGGGT TTAAGGACAG TTTTGATCCA
    CAGGGCCTGT CTGCACTAAC AGTCAGCAAC CCCAAAGGAT GGGTAACCTT CGAGGAAGAA
    GAGGACTTTG GTGTGACAGG GAAGTCAGGG TCCACTCGCC CAGACGTTTT CCTGGGTAAG
    CAGCTGAGCT CGTCTCCTGG CTCCAAGGTG ATGCTTGGTG ATGACTGGGG TAGGAGTACC
    AATGTGTCTC TCTGTGTGTT GCCAGCAAGA CCACCACCCC CACCGCCTGT CCCTCTGCTC
    CCACCTGGCA CCACCCCTCC CGCAGACGCT TTCACAGCCC TGGCCTCTAA GGCATCACCC
    ACGCTAGACT TGACAGAAAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201145.2
    CDS29..4852
    Translation

    Target ORF information:

    RefSeq Version XM_005201088.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201088.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGGGCTA GGAGTCCTGC ACCTGCTAGA AAAGAGTTTG GAGGTGTTGG AGCCCCTCCC
    AGTCCGGGGG TAGCTAGGCG AGAGATGGAA GCACCCAAAA GCCCAGGAAC GACCCGGAGA
    GATAATCTAG GACGCAGCCA GCTTCCACCT CAAGCCGGAC TGCCAGGCCC GGGACTTGCT
    GGACACAGTG CAGCCAGACC GATTATTCCG CCCCGTGCTG GAGTCATCAG CGCCCCCCAG
    AGCCACGCAC GGGTGTCTGC TGGGAGACTG ACTCCTGAAA GCCAAAGCAA AACCTCAGAA
    GTACTGAAAG GGCCAGCTCT TCTTCCCGAG CCCCTGAAGC CTCAGGCCGC CCTTCCTGTG
    CCGCCTTCTC TTGCGCCACC TTCTCTTGCG CCACCTGTTC AGAAGATGCA GGAGCCTCTC
    ATCCCGGTGG CCGCACCTCT GGCCCAGGCT GCCCTGCAGC CCAGCCTGGA AACGCCCCCG
    CAGCCACCCC CTCGGAGCAG GTCGTCCCAC AGCTTGCCTT CTGAGCCTCC GGCGCAGCCG
    CAGGTGAAAA CAAACGGAGT CTCCGCCGTC AGACTGGACT CGCCATTAAA GAGTGACCCA
    TTTGAAGACT TGTCATTGAA CCTGCTTGCT GTATCAAAGG CTCAGCCATC TGTTCACACC
    CCAAACCCAA GGGGGTTGAC GCCGTTGCCT TCTGCAGCCC CAAGTAACAC CAACACTCTG
    AGTTCTGTAA GCTGCATGCC GACAATGCCT CCAATTCCAG CCCGGAGTAA ATCCCAGGAA
    AACACGCGAT GTTCCCCAAA CCCATTCATC CCAAGCTCAA GCAGCACAAA TCCTTTCACC
    GACAGGACCG CCGCTCCTGG AAACCCCTTT CGAGCTGAGT CTCAAGAATC AGAGGCCACT
    TCATGGTTCT CCAAAGAAGA GCCTGTTGCT CCGAGTCCAT TCTCTTCGCT GAGGCCTCTG
    GATCAGAACA GTAGCAAGCC TTCATCCTCC CTGGATGGGT TTAAGGACAG TTTTGATCCA
    CAGGGCCTGT CTGCACTAAC AGTCAGCAAC CCCAAAGGAT GGGTAACCTT CGAGGAAGAA
    GAGGACTTTG GTGTGACAGG GAAGTCAGGG TCCACTCGCC CAGACGTTTT CCTGGGTAAG
    CAGCTGAGCT CGTCTCCTGG CTCCAAGGTG ATGCTTGGTG ATGACTGGGG TAGGAGTACC
    AATGTGTCTC TCTGTGTGTT GCCAGCAAGA CCACCACCCC CACCGCCTGT CCCTCTGCTC
    CCACCTGGCA CCACCCCTCC CGCAGACGCT TTCACAGCCC TGGCCTCTAA GGCATCACCC
    ACGCTAGACT TGACAGAAAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45327
    Clone ID Related Accession (Same CDS sequence) XM_005201088.3 , XM_005201088.4
    Accession Version XM_005201088.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4824bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201088.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGGGCTA GGAGTCCTGC ACCTGCTAGA AAAGAGTTTG GAGGTGTTGG AGCCCCTCCC
    AGTCCGGGGG TAGCTAGGCG AGAGATGGAA GCACCCAAAA GCCCAGGAAC GACCCGGAGA
    GATAATCTAG GACGCAGCCA GCTTCCACCT CAAGCCGGAC TGCCAGGCCC GGGACTTGCT
    GGACACAGTG CAGCCAGACC GATTATTCCG CCCCGTGCTG GAGTCATCAG CGCCCCCCAG
    AGCCACGCAC GGGTGTCTGC TGGGAGACTG ACTCCTGAAA GCCAAAGCAA AACCTCAGAA
    GTACTGAAAG GGCCAGCTCT TCTTCCCGAG CCCCTGAAGC CTCAGGCCGC CCTTCCTGTG
    CCGCCTTCTC TTGCGCCACC TTCTCTTGCG CCACCTGTTC AGAAGATGCA GGAGCCTCTC
    ATCCCGGTGG CCGCACCTCT GGCCCAGGCT GCCCTGCAGC CCAGCCTGGA AACGCCCCCG
    CAGCCACCCC CTCGGAGCAG GTCGTCCCAC AGCTTGCCTT CTGAGCCTCC GGCGCAGCCG
    CAGGTGAAAA CAAACGGAGT CTCCGCCGTC AGACTGGACT CGCCATTAAA GAGTGACCCA
    TTTGAAGACT TGTCATTGAA CCTGCTTGCT GTATCAAAGG CTCAGCCATC TGTTCACACC
    CCAAACCCAA GGGGGTTGAC GCCGTTGCCT TCTGCAGCCC CAAGTAACAC CAACACTCTG
    AGTTCTGTAA GCTGCATGCC GACAATGCCT CCAATTCCAG CCCGGAGTAA ATCCCAGGAA
    AACACGCGAT GTTCCCCAAA CCCATTCATC CCAAGCTCAA GCAGCACAAA TCCTTTCACC
    GACAGGACCG CCGCTCCTGG AAACCCCTTT CGAGCTGAGT CTCAAGAATC AGAGGCCACT
    TCATGGTTCT CCAAAGAAGA GCCTGTTGCT CCGAGTCCAT TCTCTTCGCT GAGGCCTCTG
    GATCAGAACA GTAGCAAGCC TTCATCCTCC CTGGATGGGT TTAAGGACAG TTTTGATCCA
    CAGGGCCTGT CTGCACTAAC AGTCAGCAAC CCCAAAGGAT GGGTAACCTT CGAGGAAGAA
    GAGGACTTTG GTGTGACAGG GAAGTCAGGG TCCACTCGCC CAGACGTTTT CCTGGGTAAG
    CAGCTGAGCT CGTCTCCTGG CTCCAAGGTG ATGCTTGGTG ATGACTGGGG TAGGAGTACC
    AATGTGTCTC TCTGTGTGTT GCCAGCAAGA CCACCACCCC CACCGCCTGT CCCTCTGCTC
    CCACCTGGCA CCACCCCTCC CGCAGACGCT TTCACAGCCC TGGCCTCTAA GGCATCACCC
    ACGCTAGACT TGACAGAAAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201145.2
    CDS16..4839
    Translation

    Target ORF information:

    RefSeq Version XM_005201088.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201088.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGGGCTA GGAGTCCTGC ACCTGCTAGA AAAGAGTTTG GAGGTGTTGG AGCCCCTCCC
    AGTCCGGGGG TAGCTAGGCG AGAGATGGAA GCACCCAAAA GCCCAGGAAC GACCCGGAGA
    GATAATCTAG GACGCAGCCA GCTTCCACCT CAAGCCGGAC TGCCAGGCCC GGGACTTGCT
    GGACACAGTG CAGCCAGACC GATTATTCCG CCCCGTGCTG GAGTCATCAG CGCCCCCCAG
    AGCCACGCAC GGGTGTCTGC TGGGAGACTG ACTCCTGAAA GCCAAAGCAA AACCTCAGAA
    GTACTGAAAG GGCCAGCTCT TCTTCCCGAG CCCCTGAAGC CTCAGGCCGC CCTTCCTGTG
    CCGCCTTCTC TTGCGCCACC TTCTCTTGCG CCACCTGTTC AGAAGATGCA GGAGCCTCTC
    ATCCCGGTGG CCGCACCTCT GGCCCAGGCT GCCCTGCAGC CCAGCCTGGA AACGCCCCCG
    CAGCCACCCC CTCGGAGCAG GTCGTCCCAC AGCTTGCCTT CTGAGCCTCC GGCGCAGCCG
    CAGGTGAAAA CAAACGGAGT CTCCGCCGTC AGACTGGACT CGCCATTAAA GAGTGACCCA
    TTTGAAGACT TGTCATTGAA CCTGCTTGCT GTATCAAAGG CTCAGCCATC TGTTCACACC
    CCAAACCCAA GGGGGTTGAC GCCGTTGCCT TCTGCAGCCC CAAGTAACAC CAACACTCTG
    AGTTCTGTAA GCTGCATGCC GACAATGCCT CCAATTCCAG CCCGGAGTAA ATCCCAGGAA
    AACACGCGAT GTTCCCCAAA CCCATTCATC CCAAGCTCAA GCAGCACAAA TCCTTTCACC
    GACAGGACCG CCGCTCCTGG AAACCCCTTT CGAGCTGAGT CTCAAGAATC AGAGGCCACT
    TCATGGTTCT CCAAAGAAGA GCCTGTTGCT CCGAGTCCAT TCTCTTCGCT GAGGCCTCTG
    GATCAGAACA GTAGCAAGCC TTCATCCTCC CTGGATGGGT TTAAGGACAG TTTTGATCCA
    CAGGGCCTGT CTGCACTAAC AGTCAGCAAC CCCAAAGGAT GGGTAACCTT CGAGGAAGAA
    GAGGACTTTG GTGTGACAGG GAAGTCAGGG TCCACTCGCC CAGACGTTTT CCTGGGTAAG
    CAGCTGAGCT CGTCTCCTGG CTCCAAGGTG ATGCTTGGTG ATGACTGGGG TAGGAGTACC
    AATGTGTCTC TCTGTGTGTT GCCAGCAAGA CCACCACCCC CACCGCCTGT CCCTCTGCTC
    CCACCTGGCA CCACCCCTCC CGCAGACGCT TTCACAGCCC TGGCCTCTAA GGCATCACCC
    ACGCTAGACT TGACAGAAAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45328
    Clone ID Related Accession (Same CDS sequence) XM_005201089.4 , XM_005201089.3
    Accession Version XM_005201089.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201089.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGCAC CCAAAAGCCC AGGAACGACC CGGAGAGATA ATCTAGGACG CAGCCAGCTT
    CCACCTCAAG CCGGACTGCC AGGCCCGGGA CTTGCTGGAC ACAGTGCAGC CAGACCGATT
    ATTCCGCCCC GTGCTGGAGT CATCAGCGCC CCCCAGAGCC ACGCACGGGT GTCTGCTGGG
    AGACTGACTC CTGAAAGCCA AAGCAAAACC TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT
    CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT CCTGTGCCGC CTTCTCTTGC GCCACCTTCT
    CTTGCGCCAC CTGTTCAGAA GATGCAGGAG CCTCTCATCC CGGTGGCCGC ACCTCTGGCC
    CAGGCTGCCC TGCAGCCCAG CCTGGAAACG CCCCCGCAGC CACCCCCTCG GAGCAGGTCG
    TCCCACAGCT TGCCTTCTGA GCCTCCGGCG CAGCCGCAGG TGAAAACAAA CGGAGTCTCC
    GCCGTCAGAC TGGACTCGCC ATTAAAGAGT GACCCATTTG AAGACTTGTC ATTGAACCTG
    CTTGCTGTAT CAAAGGCTCA GCCATCTGTT CACACCCCAA ACCCAAGGGG GTTGACGCCG
    TTGCCTTCTG CAGCCCCAAG TAACACCAAC ACTCTGAGTT CTGTAAGCTG CATGCCGACA
    ATGCCTCCAA TTCCAGCCCG GAGTAAATCC CAGGAAAACA CGCGATGTTC CCCAAACCCA
    TTCATCCCAA GCTCAAGCAG CACAAATCCT TTCACCGACA GGACCGCCGC TCCTGGAAAC
    CCCTTTCGAG CTGAGTCTCA AGAATCAGAG GCCACTTCAT GGTTCTCCAA AGAAGAGCCT
    GTTGCTCCGA GTCCATTCTC TTCGCTGAGG CCTCTGGATC AGAACAGTAG CAAGCCTTCA
    TCCTCCCTGG ATGGGTTTAA GGACAGTTTT GATCCACAGG GCCTGTCTGC ACTAACAGTC
    AGCAACCCCA AAGGATGGGT AACCTTCGAG GAAGAAGAGG ACTTTGGTGT GACAGGGAAG
    TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC
    AAGGTGATGC TTGGTGATGA CTGGGGTAGG AGTACCAATG TGTCTCTCTG TGTGTTGCCA
    GCAAGACCAC CACCCCCACC GCCTGTCCCT CTGCTCCCAC CTGGCACCAC CCCTCCCGCA
    GACGCTTTCA CAGCCCTGGC CTCTAAGGCA TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201146.2
    CDS16..4815
    Translation

    Target ORF information:

    RefSeq Version XM_005201089.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201089.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGCAC CCAAAAGCCC AGGAACGACC CGGAGAGATA ATCTAGGACG CAGCCAGCTT
    CCACCTCAAG CCGGACTGCC AGGCCCGGGA CTTGCTGGAC ACAGTGCAGC CAGACCGATT
    ATTCCGCCCC GTGCTGGAGT CATCAGCGCC CCCCAGAGCC ACGCACGGGT GTCTGCTGGG
    AGACTGACTC CTGAAAGCCA AAGCAAAACC TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT
    CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT CCTGTGCCGC CTTCTCTTGC GCCACCTTCT
    CTTGCGCCAC CTGTTCAGAA GATGCAGGAG CCTCTCATCC CGGTGGCCGC ACCTCTGGCC
    CAGGCTGCCC TGCAGCCCAG CCTGGAAACG CCCCCGCAGC CACCCCCTCG GAGCAGGTCG
    TCCCACAGCT TGCCTTCTGA GCCTCCGGCG CAGCCGCAGG TGAAAACAAA CGGAGTCTCC
    GCCGTCAGAC TGGACTCGCC ATTAAAGAGT GACCCATTTG AAGACTTGTC ATTGAACCTG
    CTTGCTGTAT CAAAGGCTCA GCCATCTGTT CACACCCCAA ACCCAAGGGG GTTGACGCCG
    TTGCCTTCTG CAGCCCCAAG TAACACCAAC ACTCTGAGTT CTGTAAGCTG CATGCCGACA
    ATGCCTCCAA TTCCAGCCCG GAGTAAATCC CAGGAAAACA CGCGATGTTC CCCAAACCCA
    TTCATCCCAA GCTCAAGCAG CACAAATCCT TTCACCGACA GGACCGCCGC TCCTGGAAAC
    CCCTTTCGAG CTGAGTCTCA AGAATCAGAG GCCACTTCAT GGTTCTCCAA AGAAGAGCCT
    GTTGCTCCGA GTCCATTCTC TTCGCTGAGG CCTCTGGATC AGAACAGTAG CAAGCCTTCA
    TCCTCCCTGG ATGGGTTTAA GGACAGTTTT GATCCACAGG GCCTGTCTGC ACTAACAGTC
    AGCAACCCCA AAGGATGGGT AACCTTCGAG GAAGAAGAGG ACTTTGGTGT GACAGGGAAG
    TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC
    AAGGTGATGC TTGGTGATGA CTGGGGTAGG AGTACCAATG TGTCTCTCTG TGTGTTGCCA
    GCAAGACCAC CACCCCCACC GCCTGTCCCT CTGCTCCCAC CTGGCACCAC CCCTCCCGCA
    GACGCTTTCA CAGCCCTGGC CTCTAAGGCA TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45328
    Clone ID Related Accession (Same CDS sequence) XM_005201089.4 , XM_005201089.3
    Accession Version XM_005201089.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGCAC CCAAAAGCCC AGGAACGACC CGGAGAGATA ATCTAGGACG CAGCCAGCTT
    CCACCTCAAG CCGGACTGCC AGGCCCGGGA CTTGCTGGAC ACAGTGCAGC CAGACCGATT
    ATTCCGCCCC GTGCTGGAGT CATCAGCGCC CCCCAGAGCC ACGCACGGGT GTCTGCTGGG
    AGACTGACTC CTGAAAGCCA AAGCAAAACC TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT
    CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT CCTGTGCCGC CTTCTCTTGC GCCACCTTCT
    CTTGCGCCAC CTGTTCAGAA GATGCAGGAG CCTCTCATCC CGGTGGCCGC ACCTCTGGCC
    CAGGCTGCCC TGCAGCCCAG CCTGGAAACG CCCCCGCAGC CACCCCCTCG GAGCAGGTCG
    TCCCACAGCT TGCCTTCTGA GCCTCCGGCG CAGCCGCAGG TGAAAACAAA CGGAGTCTCC
    GCCGTCAGAC TGGACTCGCC ATTAAAGAGT GACCCATTTG AAGACTTGTC ATTGAACCTG
    CTTGCTGTAT CAAAGGCTCA GCCATCTGTT CACACCCCAA ACCCAAGGGG GTTGACGCCG
    TTGCCTTCTG CAGCCCCAAG TAACACCAAC ACTCTGAGTT CTGTAAGCTG CATGCCGACA
    ATGCCTCCAA TTCCAGCCCG GAGTAAATCC CAGGAAAACA CGCGATGTTC CCCAAACCCA
    TTCATCCCAA GCTCAAGCAG CACAAATCCT TTCACCGACA GGACCGCCGC TCCTGGAAAC
    CCCTTTCGAG CTGAGTCTCA AGAATCAGAG GCCACTTCAT GGTTCTCCAA AGAAGAGCCT
    GTTGCTCCGA GTCCATTCTC TTCGCTGAGG CCTCTGGATC AGAACAGTAG CAAGCCTTCA
    TCCTCCCTGG ATGGGTTTAA GGACAGTTTT GATCCACAGG GCCTGTCTGC ACTAACAGTC
    AGCAACCCCA AAGGATGGGT AACCTTCGAG GAAGAAGAGG ACTTTGGTGT GACAGGGAAG
    TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC
    AAGGTGATGC TTGGTGATGA CTGGGGTAGG AGTACCAATG TGTCTCTCTG TGTGTTGCCA
    GCAAGACCAC CACCCCCACC GCCTGTCCCT CTGCTCCCAC CTGGCACCAC CCCTCCCGCA
    GACGCTTTCA CAGCCCTGGC CTCTAAGGCA TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201146.2
    CDS29..4828
    Translation

    Target ORF information:

    RefSeq Version XM_005201089.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201089.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGCAC CCAAAAGCCC AGGAACGACC CGGAGAGATA ATCTAGGACG CAGCCAGCTT
    CCACCTCAAG CCGGACTGCC AGGCCCGGGA CTTGCTGGAC ACAGTGCAGC CAGACCGATT
    ATTCCGCCCC GTGCTGGAGT CATCAGCGCC CCCCAGAGCC ACGCACGGGT GTCTGCTGGG
    AGACTGACTC CTGAAAGCCA AAGCAAAACC TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT
    CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT CCTGTGCCGC CTTCTCTTGC GCCACCTTCT
    CTTGCGCCAC CTGTTCAGAA GATGCAGGAG CCTCTCATCC CGGTGGCCGC ACCTCTGGCC
    CAGGCTGCCC TGCAGCCCAG CCTGGAAACG CCCCCGCAGC CACCCCCTCG GAGCAGGTCG
    TCCCACAGCT TGCCTTCTGA GCCTCCGGCG CAGCCGCAGG TGAAAACAAA CGGAGTCTCC
    GCCGTCAGAC TGGACTCGCC ATTAAAGAGT GACCCATTTG AAGACTTGTC ATTGAACCTG
    CTTGCTGTAT CAAAGGCTCA GCCATCTGTT CACACCCCAA ACCCAAGGGG GTTGACGCCG
    TTGCCTTCTG CAGCCCCAAG TAACACCAAC ACTCTGAGTT CTGTAAGCTG CATGCCGACA
    ATGCCTCCAA TTCCAGCCCG GAGTAAATCC CAGGAAAACA CGCGATGTTC CCCAAACCCA
    TTCATCCCAA GCTCAAGCAG CACAAATCCT TTCACCGACA GGACCGCCGC TCCTGGAAAC
    CCCTTTCGAG CTGAGTCTCA AGAATCAGAG GCCACTTCAT GGTTCTCCAA AGAAGAGCCT
    GTTGCTCCGA GTCCATTCTC TTCGCTGAGG CCTCTGGATC AGAACAGTAG CAAGCCTTCA
    TCCTCCCTGG ATGGGTTTAA GGACAGTTTT GATCCACAGG GCCTGTCTGC ACTAACAGTC
    AGCAACCCCA AAGGATGGGT AACCTTCGAG GAAGAAGAGG ACTTTGGTGT GACAGGGAAG
    TCAGGGTCCA CTCGCCCAGA CGTTTTCCTG GGTAAGCAGC TGAGCTCGTC TCCTGGCTCC
    AAGGTGATGC TTGGTGATGA CTGGGGTAGG AGTACCAATG TGTCTCTCTG TGTGTTGCCA
    GCAAGACCAC CACCCCCACC GCCTGTCCCT CTGCTCCCAC CTGGCACCAC CCCTCCCGCA
    GACGCTTTCA CAGCCCTGGC CTCTAAGGCA TCACCCACGC TAGACTTGAC AGAAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45329
    Clone ID Related Accession (Same CDS sequence) XM_005201090.3
    Accession Version XM_005201090.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GTTAAAAGAT GGTGCTCGTT CAGTTAGTAG AACAATTCAG
    AATAACTTCT TTGACAGCTC CAAGCAAGAA GCAATTGATG TTTTGCTCCT GGGAAATACT
    CTAAATAGTG ATTTAGCTGA CAAAGCTCGA GCCCTTTTAA CTACTGGAAG TTTGCGTGTT
    TCTGAACAGA CGTTACAGTC AGCATCTTCT AAAGTACTAA AGAGTATGTG TGAGAATTTC
    TACAAGTATT CAAAGCCCAA GAAAATTCGA GTATGTGTGG GCACATGGAA CGTGAATGGC
    GGGAAGCAGT TTCGCAGCAT AGCTTTTAAG AATCAGACCC TTACGGACTG GCTTCTTGAT
    GCCCCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA GAAGTAAACC AATGGATATA
    TTTGCAATTG GTTTTGAGGA AATGGTAGAG CTGAATGCTG GAAACATTGT GAATGCAAGC
    ACAACAAATC AGAAGCTCTG GGCTGCGGAA CTTCAGAAGA CCATCTCCAG AGACAACAAG
    TATGTGCTGC TGGCCTCTGA GCAGTTGGTG GGCGTCTGTC TGTTTGTTTT TATCAGACCA
    CAGCACGCTC CCTTCATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG AATGGGAGGC
    GCAACTGGAA ATAAGGGAGC AGTTGCAATA CGAATGCTGT TCCACACCAC AAGCCTCTGC
    TTTGTCTGCA GCCACTTCGC TGCGGGACAA TCCCAAGTCA AAGAACGAAA TGATGATTTT
    GTAGAAATAG CGCGGAAGTT GAGTTTTCCA ATGGGAAGGC TGCTCTTCTC CCATGACTAT
    GTGTTTTGGT GTGGCGATTT CAACTACCGA ATCGATCTCC CTAATGAGGA AGTGAAAGAG
    CTTATCAGAC AGCAAAACTG GGATTCTCTT ATCGCAGGAG ATCAGCTTAT CAATCAGAAA
    AATGCTGGAC AGATTTTTAG AGGATTTTTA GAAGGAAAAG TGACCTTTGC TCCAACGTAT
    AAATACGACT TGTTTTCTGA TGACTACGAC ACTAGTGAAA AGTGCCGCAC CCCTGCATGG
    ACAGACCGTG TCCTCTGGAG AAGACGGAAG TGGCCTTTTG ATAGATCAGC TGAAGATTTA
    GATCTCCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATCC TCTACACATG GACTCCTGGC
    ACTTTGCTGC ACTACGGAAG GGCTGAGCTG AAGACTTCTG ACCATAGGCC TGTTGTTGCC
    CTGATCGATA TTGATATATT TGAAGTTGAA GCTGAAGAGA GGCAAAACAT TTATAAAGAA
    GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACGGTGTTGG TCTCAATCAA AAGCTCTTTA
    CCAGAAAATA ATTTTTTCAA CGATGCTTTG ATTGATGAGC TTTTACAGCA GTTTACAAAT
    TTCGGTGAAG TTATACTCAT AAGATTTGTG GAAGATAAAA TGTGGGTTAC GTTTTTAGAG
    GGAAGCTCTG CCTTGAATGT TCTGAACCTG AATGGGAAAG AGTTACTGGG TAGGACAATA
    ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG AAGAAGAAAT GAGCTTAGAG
    AAAATCAACG TCCCCTTGCC ATCATCAACC AGCTCCACCC TCCTAGGTGA AGACGCGGAG
    GTCACGGCCG ACTTCGATAT GGAAGGTGAT GTGGATGACT ATAGTGCTGA GGTAGAGGAG
    ATCCTTCCTC AGCATCTCCA GCCGTCTTCC AGTTCTGGCC TTGGCACGTC CCCCGGTTCT
    TCACCCCGGA CCAGTCCCTG CCAGTCACCT ACCATATCGG AGGGGCCTGT GCCTTCCCTC
    CCAGTGAGAC CAAGTCGAGC TCCGTCCAGA ACGCCCGGGC CCCCTGCTTC ACAAAGTTCT
    CCTGTTGACA CTCTGCCGGC AACACAGCTG CAGCAGAAAG ATTCTTCCCA GACCCTGGAA
    CCCAAGCGGC CTCCCCCTCC CCGCCCGGTT GCTCCTCCTG CACGTCCAGC TCCTCCACAA
    CGACCACCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCTG CTAGAAAAGA GTTTGGAGCA
    CCCAAAAGCC CAGGAACGAC CCGGAGAGAT AATCTAGGAC GCAGCCAGCT TCCACCTCAA
    GCCGGACTGC CAGGCCCGGG ACTTGCTGGA CACAGTGCAG CCAGACCGAT TATTCCGCCC
    CGTGCTGGAG TCATCAGCGC CCCCCAGAGC CACGCACGGG TGTCTGCTGG GAGACTGACT
    CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC
    CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG CCTTCTCTTG CGCCACCTTC TCTTGCGCCA
    CCTGTTCAGA AGATGCAGGA GCCTCTCATC CCGGTGGCCG CACCTCTGGC CCAGGCTGCC
    CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG CCACCCCCTC GGAGCAGGTC GTCCCACAGC
    TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG GTGAAAACAA ACGGAGTCTC CGCCGTCAGA
    CTGGACTCGC CATTAAAGAG TGACCCATTT GAAGACTTGT CATTGAACCT GCTTGCTGTA
    TCAAAGGCTC AGCCATCTGT TCACACCCCA AACCCAAGGG GGTTGACGCC GTTGCCTTCT
    GCAGCCCCAA GTAACACCAA CACTCTGAGT TCTGTAAGCT GCATGCCGAC AATGCCTCCA
    ATTCCAGCCC GGAGTAAATC CCAGGAAAAC ACGCGATGTT CCCCAAACCC ATTCATCCCA
    AGCTCAAGCA GCACAAATCC TTTCACCGAC AGGACCGCCG CTCCTGGAAA CCCCTTTCGA
    GCTGAGTCTC AAGAATCAGA GGCCACTTCA TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG
    AGTCCATTCT CTTCGCTGAG GCCTCTGGAT CAGAACAGTA GCAAGCCTTC ATCCTCCCTG
    GATGGGTTTA AGGACAGTTT TGATCCACAG GGCCTGTCTG CACTAACAGT CAGCAACCCC
    AAAGGATGGG TAACCTTCGA GGAAGAAGAG GACTTTGGTG TGACAGGGAA GTCAGGGTCC
    ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG CTGAGCTCGT CTCCTGGCTC CAAGGTGATG
    CTTGGTGATG ACTGGGGTAG GAGTACCAAT GTGTCTCTCT GTGTGTTGCC AGCAAGACCA
    CCACCCCCAC CGCCTGTCCC TCTGCTCCCA CCTGGCACCA CCCCTCCCGC AGACGCTTTC
    ACAGCCCTGG CCTCTAAGGC ATCACCCACG CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201147.2
    CDS29..4819
    Translation

    Target ORF information:

    RefSeq Version XM_005201090.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201090.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GTTAAAAGAT GGTGCTCGTT CAGTTAGTAG AACAATTCAG
    AATAACTTCT TTGACAGCTC CAAGCAAGAA GCAATTGATG TTTTGCTCCT GGGAAATACT
    CTAAATAGTG ATTTAGCTGA CAAAGCTCGA GCCCTTTTAA CTACTGGAAG TTTGCGTGTT
    TCTGAACAGA CGTTACAGTC AGCATCTTCT AAAGTACTAA AGAGTATGTG TGAGAATTTC
    TACAAGTATT CAAAGCCCAA GAAAATTCGA GTATGTGTGG GCACATGGAA CGTGAATGGC
    GGGAAGCAGT TTCGCAGCAT AGCTTTTAAG AATCAGACCC TTACGGACTG GCTTCTTGAT
    GCCCCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA GAAGTAAACC AATGGATATA
    TTTGCAATTG GTTTTGAGGA AATGGTAGAG CTGAATGCTG GAAACATTGT GAATGCAAGC
    ACAACAAATC AGAAGCTCTG GGCTGCGGAA CTTCAGAAGA CCATCTCCAG AGACAACAAG
    TATGTGCTGC TGGCCTCTGA GCAGTTGGTG GGCGTCTGTC TGTTTGTTTT TATCAGACCA
    CAGCACGCTC CCTTCATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG AATGGGAGGC
    GCAACTGGAA ATAAGGGAGC AGTTGCAATA CGAATGCTGT TCCACACCAC AAGCCTCTGC
    TTTGTCTGCA GCCACTTCGC TGCGGGACAA TCCCAAGTCA AAGAACGAAA TGATGATTTT
    GTAGAAATAG CGCGGAAGTT GAGTTTTCCA ATGGGAAGGC TGCTCTTCTC CCATGACTAT
    GTGTTTTGGT GTGGCGATTT CAACTACCGA ATCGATCTCC CTAATGAGGA AGTGAAAGAG
    CTTATCAGAC AGCAAAACTG GGATTCTCTT ATCGCAGGAG ATCAGCTTAT CAATCAGAAA
    AATGCTGGAC AGATTTTTAG AGGATTTTTA GAAGGAAAAG TGACCTTTGC TCCAACGTAT
    AAATACGACT TGTTTTCTGA TGACTACGAC ACTAGTGAAA AGTGCCGCAC CCCTGCATGG
    ACAGACCGTG TCCTCTGGAG AAGACGGAAG TGGCCTTTTG ATAGATCAGC TGAAGATTTA
    GATCTCCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATCC TCTACACATG GACTCCTGGC
    ACTTTGCTGC ACTACGGAAG GGCTGAGCTG AAGACTTCTG ACCATAGGCC TGTTGTTGCC
    CTGATCGATA TTGATATATT TGAAGTTGAA GCTGAAGAGA GGCAAAACAT TTATAAAGAA
    GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACGGTGTTGG TCTCAATCAA AAGCTCTTTA
    CCAGAAAATA ATTTTTTCAA CGATGCTTTG ATTGATGAGC TTTTACAGCA GTTTACAAAT
    TTCGGTGAAG TTATACTCAT AAGATTTGTG GAAGATAAAA TGTGGGTTAC GTTTTTAGAG
    GGAAGCTCTG CCTTGAATGT TCTGAACCTG AATGGGAAAG AGTTACTGGG TAGGACAATA
    ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG AAGAAGAAAT GAGCTTAGAG
    AAAATCAACG TCCCCTTGCC ATCATCAACC AGCTCCACCC TCCTAGGTGA AGACGCGGAG
    GTCACGGCCG ACTTCGATAT GGAAGGTGAT GTGGATGACT ATAGTGCTGA GGTAGAGGAG
    ATCCTTCCTC AGCATCTCCA GCCGTCTTCC AGTTCTGGCC TTGGCACGTC CCCCGGTTCT
    TCACCCCGGA CCAGTCCCTG CCAGTCACCT ACCATATCGG AGGGGCCTGT GCCTTCCCTC
    CCAGTGAGAC CAAGTCGAGC TCCGTCCAGA ACGCCCGGGC CCCCTGCTTC ACAAAGTTCT
    CCTGTTGACA CTCTGCCGGC AACACAGCTG CAGCAGAAAG ATTCTTCCCA GACCCTGGAA
    CCCAAGCGGC CTCCCCCTCC CCGCCCGGTT GCTCCTCCTG CACGTCCAGC TCCTCCACAA
    CGACCACCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCTG CTAGAAAAGA GTTTGGAGCA
    CCCAAAAGCC CAGGAACGAC CCGGAGAGAT AATCTAGGAC GCAGCCAGCT TCCACCTCAA
    GCCGGACTGC CAGGCCCGGG ACTTGCTGGA CACAGTGCAG CCAGACCGAT TATTCCGCCC
    CGTGCTGGAG TCATCAGCGC CCCCCAGAGC CACGCACGGG TGTCTGCTGG GAGACTGACT
    CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC
    CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG CCTTCTCTTG CGCCACCTTC TCTTGCGCCA
    CCTGTTCAGA AGATGCAGGA GCCTCTCATC CCGGTGGCCG CACCTCTGGC CCAGGCTGCC
    CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG CCACCCCCTC GGAGCAGGTC GTCCCACAGC
    TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG GTGAAAACAA ACGGAGTCTC CGCCGTCAGA
    CTGGACTCGC CATTAAAGAG TGACCCATTT GAAGACTTGT CATTGAACCT GCTTGCTGTA
    TCAAAGGCTC AGCCATCTGT TCACACCCCA AACCCAAGGG GGTTGACGCC GTTGCCTTCT
    GCAGCCCCAA GTAACACCAA CACTCTGAGT TCTGTAAGCT GCATGCCGAC AATGCCTCCA
    ATTCCAGCCC GGAGTAAATC CCAGGAAAAC ACGCGATGTT CCCCAAACCC ATTCATCCCA
    AGCTCAAGCA GCACAAATCC TTTCACCGAC AGGACCGCCG CTCCTGGAAA CCCCTTTCGA
    GCTGAGTCTC AAGAATCAGA GGCCACTTCA TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG
    AGTCCATTCT CTTCGCTGAG GCCTCTGGAT CAGAACAGTA GCAAGCCTTC ATCCTCCCTG
    GATGGGTTTA AGGACAGTTT TGATCCACAG GGCCTGTCTG CACTAACAGT CAGCAACCCC
    AAAGGATGGG TAACCTTCGA GGAAGAAGAG GACTTTGGTG TGACAGGGAA GTCAGGGTCC
    ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG CTGAGCTCGT CTCCTGGCTC CAAGGTGATG
    CTTGGTGATG ACTGGGGTAG GAGTACCAAT GTGTCTCTCT GTGTGTTGCC AGCAAGACCA
    CCACCCCCAC CGCCTGTCCC TCTGCTCCCA CCTGGCACCA CCCCTCCCGC AGACGCTTTC
    ACAGCCCTGG CCTCTAAGGC ATCACCCACG CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45330
    Clone ID Related Accession (Same CDS sequence) XM_010800891.2 , XM_010800891.3
    Accession Version XM_010800891.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800891.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGCA CCCAAAAGCC CAGGAACGAC CCGGAGAGAT
    AATCTAGGAC GCAGCCAGCT TCCACCTCAA GCCGGACTGC CAGGCCCGGG ACTTGCTGGA
    CACAGTGCAG CCAGACCGAT TATTCCGCCC CGTGCTGGAG TCATCAGCGC CCCCCAGAGC
    CACGCACGGG TGTCTGCTGG GAGACTGACT CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA
    CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG
    CCTTCTCTTG CGCCACCTTC TCTTGCGCCA CCTGTTCAGA AGATGCAGGA GCCTCTCATC
    CCGGTGGCCG CACCTCTGGC CCAGGCTGCC CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG
    CCACCCCCTC GGAGCAGGTC GTCCCACAGC TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG
    GTGAAAACAA ACGGAGTCTC CGCCGTCAGA CTGGACTCGC CATTAAAGAG TGACCCATTT
    GAAGACTTGT CATTGAACCT GCTTGCTGTA TCAAAGGCTC AGCCATCTGT TCACACCCCA
    AACCCAAGGG GGTTGACGCC GTTGCCTTCT GCAGCCCCAA GTAACACCAA CACTCTGAGT
    TCTGTAAGCT GCATGCCGAC AATGCCTCCA ATTCCAGCCC GGAGTAAATC CCAGGAAAAC
    ACGCGATGTT CCCCAAACCC ATTCATCCCA AGCTCAAGCA GCACAAATCC TTTCACCGAC
    AGGACCGCCG CTCCTGGAAA CCCCTTTCGA GCTGAGTCTC AAGAATCAGA GGCCACTTCA
    TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG AGTCCATTCT CTTCGCTGAG GCCTCTGGAT
    CAGAACAGTA GCAAGCCTTC ATCCTCCCTG GATGGGTTTA AGGACAGTTT TGATCCACAG
    GGCCTGTCTG CACTAACAGT CAGCAACCCC AAAGGATGGG TAACCTTCGA GGAAGAAGAG
    GACTTTGGTG TGACAGGGAA GTCAGGGTCC ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG
    CTGAGCTCGT CTCCTGGCTC CAAGGTGATG CTTGGTGATG ACTGGGGTAG GAGTACCAAT
    GTGTCTCTCT GTGTGTTGCC AGCAAGACCA CCACCCCCAC CGCCTGTCCC TCTGCTCCCA
    CCTGGCACCA CCCCTCCCGC AGACGCTTTC ACAGCCCTGG CCTCTAAGGC ATCACCCACG
    CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799193.1
    CDS29..4789
    Translation

    Target ORF information:

    RefSeq Version XM_010800891.2
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010800891.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGCA CCCAAAAGCC CAGGAACGAC CCGGAGAGAT
    AATCTAGGAC GCAGCCAGCT TCCACCTCAA GCCGGACTGC CAGGCCCGGG ACTTGCTGGA
    CACAGTGCAG CCAGACCGAT TATTCCGCCC CGTGCTGGAG TCATCAGCGC CCCCCAGAGC
    CACGCACGGG TGTCTGCTGG GAGACTGACT CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA
    CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG
    CCTTCTCTTG CGCCACCTTC TCTTGCGCCA CCTGTTCAGA AGATGCAGGA GCCTCTCATC
    CCGGTGGCCG CACCTCTGGC CCAGGCTGCC CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG
    CCACCCCCTC GGAGCAGGTC GTCCCACAGC TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG
    GTGAAAACAA ACGGAGTCTC CGCCGTCAGA CTGGACTCGC CATTAAAGAG TGACCCATTT
    GAAGACTTGT CATTGAACCT GCTTGCTGTA TCAAAGGCTC AGCCATCTGT TCACACCCCA
    AACCCAAGGG GGTTGACGCC GTTGCCTTCT GCAGCCCCAA GTAACACCAA CACTCTGAGT
    TCTGTAAGCT GCATGCCGAC AATGCCTCCA ATTCCAGCCC GGAGTAAATC CCAGGAAAAC
    ACGCGATGTT CCCCAAACCC ATTCATCCCA AGCTCAAGCA GCACAAATCC TTTCACCGAC
    AGGACCGCCG CTCCTGGAAA CCCCTTTCGA GCTGAGTCTC AAGAATCAGA GGCCACTTCA
    TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG AGTCCATTCT CTTCGCTGAG GCCTCTGGAT
    CAGAACAGTA GCAAGCCTTC ATCCTCCCTG GATGGGTTTA AGGACAGTTT TGATCCACAG
    GGCCTGTCTG CACTAACAGT CAGCAACCCC AAAGGATGGG TAACCTTCGA GGAAGAAGAG
    GACTTTGGTG TGACAGGGAA GTCAGGGTCC ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG
    CTGAGCTCGT CTCCTGGCTC CAAGGTGATG CTTGGTGATG ACTGGGGTAG GAGTACCAAT
    GTGTCTCTCT GTGTGTTGCC AGCAAGACCA CCACCCCCAC CGCCTGTCCC TCTGCTCCCA
    CCTGGCACCA CCCCTCCCGC AGACGCTTTC ACAGCCCTGG CCTCTAAGGC ATCACCCACG
    CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45330
    Clone ID Related Accession (Same CDS sequence) XM_010800891.2 , XM_010800891.3
    Accession Version XM_010800891.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010800891.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGCA CCCAAAAGCC CAGGAACGAC CCGGAGAGAT
    AATCTAGGAC GCAGCCAGCT TCCACCTCAA GCCGGACTGC CAGGCCCGGG ACTTGCTGGA
    CACAGTGCAG CCAGACCGAT TATTCCGCCC CGTGCTGGAG TCATCAGCGC CCCCCAGAGC
    CACGCACGGG TGTCTGCTGG GAGACTGACT CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA
    CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG
    CCTTCTCTTG CGCCACCTTC TCTTGCGCCA CCTGTTCAGA AGATGCAGGA GCCTCTCATC
    CCGGTGGCCG CACCTCTGGC CCAGGCTGCC CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG
    CCACCCCCTC GGAGCAGGTC GTCCCACAGC TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG
    GTGAAAACAA ACGGAGTCTC CGCCGTCAGA CTGGACTCGC CATTAAAGAG TGACCCATTT
    GAAGACTTGT CATTGAACCT GCTTGCTGTA TCAAAGGCTC AGCCATCTGT TCACACCCCA
    AACCCAAGGG GGTTGACGCC GTTGCCTTCT GCAGCCCCAA GTAACACCAA CACTCTGAGT
    TCTGTAAGCT GCATGCCGAC AATGCCTCCA ATTCCAGCCC GGAGTAAATC CCAGGAAAAC
    ACGCGATGTT CCCCAAACCC ATTCATCCCA AGCTCAAGCA GCACAAATCC TTTCACCGAC
    AGGACCGCCG CTCCTGGAAA CCCCTTTCGA GCTGAGTCTC AAGAATCAGA GGCCACTTCA
    TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG AGTCCATTCT CTTCGCTGAG GCCTCTGGAT
    CAGAACAGTA GCAAGCCTTC ATCCTCCCTG GATGGGTTTA AGGACAGTTT TGATCCACAG
    GGCCTGTCTG CACTAACAGT CAGCAACCCC AAAGGATGGG TAACCTTCGA GGAAGAAGAG
    GACTTTGGTG TGACAGGGAA GTCAGGGTCC ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG
    CTGAGCTCGT CTCCTGGCTC CAAGGTGATG CTTGGTGATG ACTGGGGTAG GAGTACCAAT
    GTGTCTCTCT GTGTGTTGCC AGCAAGACCA CCACCCCCAC CGCCTGTCCC TCTGCTCCCA
    CCTGGCACCA CCCCTCCCGC AGACGCTTTC ACAGCCCTGG CCTCTAAGGC ATCACCCACG
    CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799193.1
    CDS16..4776
    Translation

    Target ORF information:

    RefSeq Version XM_010800891.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010800891.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGCA CCCAAAAGCC CAGGAACGAC CCGGAGAGAT
    AATCTAGGAC GCAGCCAGCT TCCACCTCAA GCCGGACTGC CAGGCCCGGG ACTTGCTGGA
    CACAGTGCAG CCAGACCGAT TATTCCGCCC CGTGCTGGAG TCATCAGCGC CCCCCAGAGC
    CACGCACGGG TGTCTGCTGG GAGACTGACT CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA
    CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG
    CCTTCTCTTG CGCCACCTTC TCTTGCGCCA CCTGTTCAGA AGATGCAGGA GCCTCTCATC
    CCGGTGGCCG CACCTCTGGC CCAGGCTGCC CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG
    CCACCCCCTC GGAGCAGGTC GTCCCACAGC TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG
    GTGAAAACAA ACGGAGTCTC CGCCGTCAGA CTGGACTCGC CATTAAAGAG TGACCCATTT
    GAAGACTTGT CATTGAACCT GCTTGCTGTA TCAAAGGCTC AGCCATCTGT TCACACCCCA
    AACCCAAGGG GGTTGACGCC GTTGCCTTCT GCAGCCCCAA GTAACACCAA CACTCTGAGT
    TCTGTAAGCT GCATGCCGAC AATGCCTCCA ATTCCAGCCC GGAGTAAATC CCAGGAAAAC
    ACGCGATGTT CCCCAAACCC ATTCATCCCA AGCTCAAGCA GCACAAATCC TTTCACCGAC
    AGGACCGCCG CTCCTGGAAA CCCCTTTCGA GCTGAGTCTC AAGAATCAGA GGCCACTTCA
    TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG AGTCCATTCT CTTCGCTGAG GCCTCTGGAT
    CAGAACAGTA GCAAGCCTTC ATCCTCCCTG GATGGGTTTA AGGACAGTTT TGATCCACAG
    GGCCTGTCTG CACTAACAGT CAGCAACCCC AAAGGATGGG TAACCTTCGA GGAAGAAGAG
    GACTTTGGTG TGACAGGGAA GTCAGGGTCC ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG
    CTGAGCTCGT CTCCTGGCTC CAAGGTGATG CTTGGTGATG ACTGGGGTAG GAGTACCAAT
    GTGTCTCTCT GTGTGTTGCC AGCAAGACCA CCACCCCCAC CGCCTGTCCC TCTGCTCCCA
    CCTGGCACCA CCCCTCCCGC AGACGCTTTC ACAGCCCTGG CCTCTAAGGC ATCACCCACG
    CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45331
    Clone ID Related Accession (Same CDS sequence) XM_010800892.2
    Accession Version XM_010800892.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010800892.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGAC GCAGCCAGCT TCCACCTCAA GCCGGACTGC CAGGCCCGGG ACTTGCTGGA
    CACAGTGCAG CCAGACCGAT TATTCCGCCC CGTGCTGGAG TCATCAGCGC CCCCCAGAGC
    CACGCACGGG TGTCTGCTGG GAGACTGACT CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA
    CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG
    CCTTCTCTTG CGCCACCTTC TCTTGCGCCA CCTGTTCAGA AGATGCAGGA GCCTCTCATC
    CCGGTGGCCG CACCTCTGGC CCAGGCTGCC CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG
    CCACCCCCTC GGAGCAGGTC GTCCCACAGC TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG
    GTGAAAACAA ACGGAGTCTC CGCCGTCAGA CTGGACTCGC CATTAAAGAG TGACCCATTT
    GAAGACTTGT CATTGAACCT GCTTGCTGTA TCAAAGGCTC AGCCATCTGT TCACACCCCA
    AACCCAAGGG GGTTGACGCC GTTGCCTTCT GCAGCCCCAA GTAACACCAA CACTCTGAGT
    TCTGTAAGCT GCATGCCGAC AATGCCTCCA ATTCCAGCCC GGAGTAAATC CCAGGAAAAC
    ACGCGATGTT CCCCAAACCC ATTCATCCCA AGCTCAAGCA GCACAAATCC TTTCACCGAC
    AGGACCGCCG CTCCTGGAAA CCCCTTTCGA GCTGAGTCTC AAGAATCAGA GGCCACTTCA
    TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG AGTCCATTCT CTTCGCTGAG GCCTCTGGAT
    CAGAACAGTA GCAAGCCTTC ATCCTCCCTG GATGGGTTTA AGGACAGTTT TGATCCACAG
    GGCCTGTCTG CACTAACAGT CAGCAACCCC AAAGGATGGG TAACCTTCGA GGAAGAAGAG
    GACTTTGGTG TGACAGGGAA GTCAGGGTCC ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG
    CTGAGCTCGT CTCCTGGCTC CAAGGTGATG CTTGGTGATG ACTGGGGTAG GAGTACCAAT
    GTGTCTCTCT GTGTGTTGCC AGCAAGACCA CCACCCCCAC CGCCTGTCCC TCTGCTCCCA
    CCTGGCACCA CCCCTCCCGC AGACGCTTTC ACAGCCCTGG CCTCTAAGGC ATCACCCACG
    CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799194.1
    CDS29..4789
    Translation

    Target ORF information:

    RefSeq Version XM_010800892.2
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010800892.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGAC GCAGCCAGCT TCCACCTCAA GCCGGACTGC CAGGCCCGGG ACTTGCTGGA
    CACAGTGCAG CCAGACCGAT TATTCCGCCC CGTGCTGGAG TCATCAGCGC CCCCCAGAGC
    CACGCACGGG TGTCTGCTGG GAGACTGACT CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA
    CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG
    CCTTCTCTTG CGCCACCTTC TCTTGCGCCA CCTGTTCAGA AGATGCAGGA GCCTCTCATC
    CCGGTGGCCG CACCTCTGGC CCAGGCTGCC CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG
    CCACCCCCTC GGAGCAGGTC GTCCCACAGC TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG
    GTGAAAACAA ACGGAGTCTC CGCCGTCAGA CTGGACTCGC CATTAAAGAG TGACCCATTT
    GAAGACTTGT CATTGAACCT GCTTGCTGTA TCAAAGGCTC AGCCATCTGT TCACACCCCA
    AACCCAAGGG GGTTGACGCC GTTGCCTTCT GCAGCCCCAA GTAACACCAA CACTCTGAGT
    TCTGTAAGCT GCATGCCGAC AATGCCTCCA ATTCCAGCCC GGAGTAAATC CCAGGAAAAC
    ACGCGATGTT CCCCAAACCC ATTCATCCCA AGCTCAAGCA GCACAAATCC TTTCACCGAC
    AGGACCGCCG CTCCTGGAAA CCCCTTTCGA GCTGAGTCTC AAGAATCAGA GGCCACTTCA
    TGGTTCTCCA AAGAAGAGCC TGTTGCTCCG AGTCCATTCT CTTCGCTGAG GCCTCTGGAT
    CAGAACAGTA GCAAGCCTTC ATCCTCCCTG GATGGGTTTA AGGACAGTTT TGATCCACAG
    GGCCTGTCTG CACTAACAGT CAGCAACCCC AAAGGATGGG TAACCTTCGA GGAAGAAGAG
    GACTTTGGTG TGACAGGGAA GTCAGGGTCC ACTCGCCCAG ACGTTTTCCT GGGTAAGCAG
    CTGAGCTCGT CTCCTGGCTC CAAGGTGATG CTTGGTGATG ACTGGGGTAG GAGTACCAAT
    GTGTCTCTCT GTGTGTTGCC AGCAAGACCA CCACCCCCAC CGCCTGTCCC TCTGCTCCCA
    CCTGGCACCA CCCCTCCCGC AGACGCTTTC ACAGCCCTGG CCTCTAAGGC ATCACCCACG
    CTAGACTTGA CAGAAAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45332
    Clone ID Related Accession (Same CDS sequence) XM_005201091.4 , XM_005201091.3
    Accession Version XM_005201091.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201091.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGA CGCAGCCAGC TTCCACCTCA AGCCGGACTG
    CCAGGCCCGG GACTTGCTGG ACACAGTGCA GCCAGACCGA TTATTCCGCC CCGTGCTGGA
    GTCATCAGCG CCCCCCAGAG CCACGCACGG GTGTCTGCTG GGAGACTGAC TCCTGAAAGC
    CAAAGCAAAA CCTCAGAAGT ACTGAAAGGG CCAGCTCTTC TTCCCGAGCC CCTGAAGCCT
    CAGGCCGCCC TTCCTGTGCC GCCTTCTCTT GCGCCACCTT CTCTTGCGCC ACCTGTTCAG
    AAGATGCAGG AGCCTCTCAT CCCGGTGGCC GCACCTCTGG CCCAGGCTGC CCTGCAGCCC
    AGCCTGGAAA CGCCCCCGCA GCCACCCCCT CGGAGCAGGT CGTCCCACAG CTTGCCTTCT
    GAGCCTCCGG CGCAGCCGCA GGTGAAAACA AACGGAGTCT CCGCCGTCAG ACTGGACTCG
    CCATTAAAGA GTGACCCATT TGAAGACTTG TCATTGAACC TGCTTGCTGT ATCAAAGGCT
    CAGCCATCTG TTCACACCCC AAACCCAAGG GGGTTGACGC CGTTGCCTTC TGCAGCCCCA
    AGTAACACCA ACACTCTGAG TTCTGTAAGC TGCATGCCGA CAATGCCTCC AATTCCAGCC
    CGGAGTAAAT CCCAGGAAAA CACGCGATGT TCCCCAAACC CATTCATCCC AAGCTCAAGC
    AGCACAAATC CTTTCACCGA CAGGACCGCC GCTCCTGGAA ACCCCTTTCG AGCTGAGTCT
    CAAGAATCAG AGGCCACTTC ATGGTTCTCC AAAGAAGAGC CTGTTGCTCC GAGTCCATTC
    TCTTCGCTGA GGCCTCTGGA TCAGAACAGT AGCAAGCCTT CATCCTCCCT GGATGGGTTT
    AAGGACAGTT TTGATCCACA GGGCCTGTCT GCACTAACAG TCAGCAACCC CAAAGGATGG
    GTAACCTTCG AGGAAGAAGA GGACTTTGGT GTGACAGGGA AGTCAGGGTC CACTCGCCCA
    GACGTTTTCC TGGGTAAGCA GCTGAGCTCG TCTCCTGGCT CCAAGGTGAT GCTTGGTGAT
    GACTGGGGTA GGAGTACCAA TGTGTCTCTC TGTGTGTTGC CAGCAAGACC ACCACCCCCA
    CCGCCTGTCC CTCTGCTCCC ACCTGGCACC ACCCCTCCCG CAGACGCTTT CACAGCCCTG
    GCCTCTAAGG CATCACCCAC GCTAGACTTG ACAGAAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201148.2
    CDS16..4737
    Translation

    Target ORF information:

    RefSeq Version XM_005201091.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201091.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGA CGCAGCCAGC TTCCACCTCA AGCCGGACTG
    CCAGGCCCGG GACTTGCTGG ACACAGTGCA GCCAGACCGA TTATTCCGCC CCGTGCTGGA
    GTCATCAGCG CCCCCCAGAG CCACGCACGG GTGTCTGCTG GGAGACTGAC TCCTGAAAGC
    CAAAGCAAAA CCTCAGAAGT ACTGAAAGGG CCAGCTCTTC TTCCCGAGCC CCTGAAGCCT
    CAGGCCGCCC TTCCTGTGCC GCCTTCTCTT GCGCCACCTT CTCTTGCGCC ACCTGTTCAG
    AAGATGCAGG AGCCTCTCAT CCCGGTGGCC GCACCTCTGG CCCAGGCTGC CCTGCAGCCC
    AGCCTGGAAA CGCCCCCGCA GCCACCCCCT CGGAGCAGGT CGTCCCACAG CTTGCCTTCT
    GAGCCTCCGG CGCAGCCGCA GGTGAAAACA AACGGAGTCT CCGCCGTCAG ACTGGACTCG
    CCATTAAAGA GTGACCCATT TGAAGACTTG TCATTGAACC TGCTTGCTGT ATCAAAGGCT
    CAGCCATCTG TTCACACCCC AAACCCAAGG GGGTTGACGC CGTTGCCTTC TGCAGCCCCA
    AGTAACACCA ACACTCTGAG TTCTGTAAGC TGCATGCCGA CAATGCCTCC AATTCCAGCC
    CGGAGTAAAT CCCAGGAAAA CACGCGATGT TCCCCAAACC CATTCATCCC AAGCTCAAGC
    AGCACAAATC CTTTCACCGA CAGGACCGCC GCTCCTGGAA ACCCCTTTCG AGCTGAGTCT
    CAAGAATCAG AGGCCACTTC ATGGTTCTCC AAAGAAGAGC CTGTTGCTCC GAGTCCATTC
    TCTTCGCTGA GGCCTCTGGA TCAGAACAGT AGCAAGCCTT CATCCTCCCT GGATGGGTTT
    AAGGACAGTT TTGATCCACA GGGCCTGTCT GCACTAACAG TCAGCAACCC CAAAGGATGG
    GTAACCTTCG AGGAAGAAGA GGACTTTGGT GTGACAGGGA AGTCAGGGTC CACTCGCCCA
    GACGTTTTCC TGGGTAAGCA GCTGAGCTCG TCTCCTGGCT CCAAGGTGAT GCTTGGTGAT
    GACTGGGGTA GGAGTACCAA TGTGTCTCTC TGTGTGTTGC CAGCAAGACC ACCACCCCCA
    CCGCCTGTCC CTCTGCTCCC ACCTGGCACC ACCCCTCCCG CAGACGCTTT CACAGCCCTG
    GCCTCTAAGG CATCACCCAC GCTAGACTTG ACAGAAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45332
    Clone ID Related Accession (Same CDS sequence) XM_005201091.4 , XM_005201091.3
    Accession Version XM_005201091.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGA CGCAGCCAGC TTCCACCTCA AGCCGGACTG
    CCAGGCCCGG GACTTGCTGG ACACAGTGCA GCCAGACCGA TTATTCCGCC CCGTGCTGGA
    GTCATCAGCG CCCCCCAGAG CCACGCACGG GTGTCTGCTG GGAGACTGAC TCCTGAAAGC
    CAAAGCAAAA CCTCAGAAGT ACTGAAAGGG CCAGCTCTTC TTCCCGAGCC CCTGAAGCCT
    CAGGCCGCCC TTCCTGTGCC GCCTTCTCTT GCGCCACCTT CTCTTGCGCC ACCTGTTCAG
    AAGATGCAGG AGCCTCTCAT CCCGGTGGCC GCACCTCTGG CCCAGGCTGC CCTGCAGCCC
    AGCCTGGAAA CGCCCCCGCA GCCACCCCCT CGGAGCAGGT CGTCCCACAG CTTGCCTTCT
    GAGCCTCCGG CGCAGCCGCA GGTGAAAACA AACGGAGTCT CCGCCGTCAG ACTGGACTCG
    CCATTAAAGA GTGACCCATT TGAAGACTTG TCATTGAACC TGCTTGCTGT ATCAAAGGCT
    CAGCCATCTG TTCACACCCC AAACCCAAGG GGGTTGACGC CGTTGCCTTC TGCAGCCCCA
    AGTAACACCA ACACTCTGAG TTCTGTAAGC TGCATGCCGA CAATGCCTCC AATTCCAGCC
    CGGAGTAAAT CCCAGGAAAA CACGCGATGT TCCCCAAACC CATTCATCCC AAGCTCAAGC
    AGCACAAATC CTTTCACCGA CAGGACCGCC GCTCCTGGAA ACCCCTTTCG AGCTGAGTCT
    CAAGAATCAG AGGCCACTTC ATGGTTCTCC AAAGAAGAGC CTGTTGCTCC GAGTCCATTC
    TCTTCGCTGA GGCCTCTGGA TCAGAACAGT AGCAAGCCTT CATCCTCCCT GGATGGGTTT
    AAGGACAGTT TTGATCCACA GGGCCTGTCT GCACTAACAG TCAGCAACCC CAAAGGATGG
    GTAACCTTCG AGGAAGAAGA GGACTTTGGT GTGACAGGGA AGTCAGGGTC CACTCGCCCA
    GACGTTTTCC TGGGTAAGCA GCTGAGCTCG TCTCCTGGCT CCAAGGTGAT GCTTGGTGAT
    GACTGGGGTA GGAGTACCAA TGTGTCTCTC TGTGTGTTGC CAGCAAGACC ACCACCCCCA
    CCGCCTGTCC CTCTGCTCCC ACCTGGCACC ACCCCTCCCG CAGACGCTTT CACAGCCCTG
    GCCTCTAAGG CATCACCCAC GCTAGACTTG ACAGAAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201148.2
    CDS29..4750
    Translation

    Target ORF information:

    RefSeq Version XM_005201091.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201091.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGA CGCAGCCAGC TTCCACCTCA AGCCGGACTG
    CCAGGCCCGG GACTTGCTGG ACACAGTGCA GCCAGACCGA TTATTCCGCC CCGTGCTGGA
    GTCATCAGCG CCCCCCAGAG CCACGCACGG GTGTCTGCTG GGAGACTGAC TCCTGAAAGC
    CAAAGCAAAA CCTCAGAAGT ACTGAAAGGG CCAGCTCTTC TTCCCGAGCC CCTGAAGCCT
    CAGGCCGCCC TTCCTGTGCC GCCTTCTCTT GCGCCACCTT CTCTTGCGCC ACCTGTTCAG
    AAGATGCAGG AGCCTCTCAT CCCGGTGGCC GCACCTCTGG CCCAGGCTGC CCTGCAGCCC
    AGCCTGGAAA CGCCCCCGCA GCCACCCCCT CGGAGCAGGT CGTCCCACAG CTTGCCTTCT
    GAGCCTCCGG CGCAGCCGCA GGTGAAAACA AACGGAGTCT CCGCCGTCAG ACTGGACTCG
    CCATTAAAGA GTGACCCATT TGAAGACTTG TCATTGAACC TGCTTGCTGT ATCAAAGGCT
    CAGCCATCTG TTCACACCCC AAACCCAAGG GGGTTGACGC CGTTGCCTTC TGCAGCCCCA
    AGTAACACCA ACACTCTGAG TTCTGTAAGC TGCATGCCGA CAATGCCTCC AATTCCAGCC
    CGGAGTAAAT CCCAGGAAAA CACGCGATGT TCCCCAAACC CATTCATCCC AAGCTCAAGC
    AGCACAAATC CTTTCACCGA CAGGACCGCC GCTCCTGGAA ACCCCTTTCG AGCTGAGTCT
    CAAGAATCAG AGGCCACTTC ATGGTTCTCC AAAGAAGAGC CTGTTGCTCC GAGTCCATTC
    TCTTCGCTGA GGCCTCTGGA TCAGAACAGT AGCAAGCCTT CATCCTCCCT GGATGGGTTT
    AAGGACAGTT TTGATCCACA GGGCCTGTCT GCACTAACAG TCAGCAACCC CAAAGGATGG
    GTAACCTTCG AGGAAGAAGA GGACTTTGGT GTGACAGGGA AGTCAGGGTC CACTCGCCCA
    GACGTTTTCC TGGGTAAGCA GCTGAGCTCG TCTCCTGGCT CCAAGGTGAT GCTTGGTGAT
    GACTGGGGTA GGAGTACCAA TGTGTCTCTC TGTGTGTTGC CAGCAAGACC ACCACCCCCA
    CCGCCTGTCC CTCTGCTCCC ACCTGGCACC ACCCCTCCCG CAGACGCTTT CACAGCCCTG
    GCCTCTAAGG CATCACCCAC GCTAGACTTG ACAGAAAGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45333
    Clone ID Related Accession (Same CDS sequence) XM_005201092.4 , XM_005201092.3
    Accession Version XM_005201092.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201092.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGACGCA GCCAGCTTCC ACCTCAAGCC GGACTGCCAG GCCCGGGACT TGCTGGACAC
    AGTGCAGCCA GACCGATTAT TCCGCCCCGT GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC
    GCACGGGTGT CTGCTGGGAG ACTGACTCCT GAAAGCCAAA GCAAAACCTC AGAAGTACTG
    AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT
    TCTCTTGCGC CACCTTCTCT TGCGCCACCT GTTCAGAAGA TGCAGGAGCC TCTCATCCCG
    GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG CAGCCCAGCC TGGAAACGCC CCCGCAGCCA
    CCCCCTCGGA GCAGGTCGTC CCACAGCTTG CCTTCTGAGC CTCCGGCGCA GCCGCAGGTG
    AAAACAAACG GAGTCTCCGC CGTCAGACTG GACTCGCCAT TAAAGAGTGA CCCATTTGAA
    GACTTGTCAT TGAACCTGCT TGCTGTATCA AAGGCTCAGC CATCTGTTCA CACCCCAAAC
    CCAAGGGGGT TGACGCCGTT GCCTTCTGCA GCCCCAAGTA ACACCAACAC TCTGAGTTCT
    GTAAGCTGCA TGCCGACAAT GCCTCCAATT CCAGCCCGGA GTAAATCCCA GGAAAACACG
    CGATGTTCCC CAAACCCATT CATCCCAAGC TCAAGCAGCA CAAATCCTTT CACCGACAGG
    ACCGCCGCTC CTGGAAACCC CTTTCGAGCT GAGTCTCAAG AATCAGAGGC CACTTCATGG
    TTCTCCAAAG AAGAGCCTGT TGCTCCGAGT CCATTCTCTT CGCTGAGGCC TCTGGATCAG
    AACAGTAGCA AGCCTTCATC CTCCCTGGAT GGGTTTAAGG ACAGTTTTGA TCCACAGGGC
    CTGTCTGCAC TAACAGTCAG CAACCCCAAA GGATGGGTAA CCTTCGAGGA AGAAGAGGAC
    TTTGGTGTGA CAGGGAAGTC AGGGTCCACT CGCCCAGACG TTTTCCTGGG TAAGCAGCTG
    AGCTCGTCTC CTGGCTCCAA GGTGATGCTT GGTGATGACT GGGGTAGGAG TACCAATGTG
    TCTCTCTGTG TGTTGCCAGC AAGACCACCA CCCCCACCGC CTGTCCCTCT GCTCCCACCT
    GGCACCACCC CTCCCGCAGA CGCTTTCACA GCCCTGGCCT CTAAGGCATC ACCCACGCTA
    GACTTGACAG AAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201149.2
    CDS16..4713
    Translation

    Target ORF information:

    RefSeq Version XM_005201092.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201092.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGACGCA GCCAGCTTCC ACCTCAAGCC GGACTGCCAG GCCCGGGACT TGCTGGACAC
    AGTGCAGCCA GACCGATTAT TCCGCCCCGT GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC
    GCACGGGTGT CTGCTGGGAG ACTGACTCCT GAAAGCCAAA GCAAAACCTC AGAAGTACTG
    AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT
    TCTCTTGCGC CACCTTCTCT TGCGCCACCT GTTCAGAAGA TGCAGGAGCC TCTCATCCCG
    GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG CAGCCCAGCC TGGAAACGCC CCCGCAGCCA
    CCCCCTCGGA GCAGGTCGTC CCACAGCTTG CCTTCTGAGC CTCCGGCGCA GCCGCAGGTG
    AAAACAAACG GAGTCTCCGC CGTCAGACTG GACTCGCCAT TAAAGAGTGA CCCATTTGAA
    GACTTGTCAT TGAACCTGCT TGCTGTATCA AAGGCTCAGC CATCTGTTCA CACCCCAAAC
    CCAAGGGGGT TGACGCCGTT GCCTTCTGCA GCCCCAAGTA ACACCAACAC TCTGAGTTCT
    GTAAGCTGCA TGCCGACAAT GCCTCCAATT CCAGCCCGGA GTAAATCCCA GGAAAACACG
    CGATGTTCCC CAAACCCATT CATCCCAAGC TCAAGCAGCA CAAATCCTTT CACCGACAGG
    ACCGCCGCTC CTGGAAACCC CTTTCGAGCT GAGTCTCAAG AATCAGAGGC CACTTCATGG
    TTCTCCAAAG AAGAGCCTGT TGCTCCGAGT CCATTCTCTT CGCTGAGGCC TCTGGATCAG
    AACAGTAGCA AGCCTTCATC CTCCCTGGAT GGGTTTAAGG ACAGTTTTGA TCCACAGGGC
    CTGTCTGCAC TAACAGTCAG CAACCCCAAA GGATGGGTAA CCTTCGAGGA AGAAGAGGAC
    TTTGGTGTGA CAGGGAAGTC AGGGTCCACT CGCCCAGACG TTTTCCTGGG TAAGCAGCTG
    AGCTCGTCTC CTGGCTCCAA GGTGATGCTT GGTGATGACT GGGGTAGGAG TACCAATGTG
    TCTCTCTGTG TGTTGCCAGC AAGACCACCA CCCCCACCGC CTGTCCCTCT GCTCCCACCT
    GGCACCACCC CTCCCGCAGA CGCTTTCACA GCCCTGGCCT CTAAGGCATC ACCCACGCTA
    GACTTGACAG AAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45333
    Clone ID Related Accession (Same CDS sequence) XM_005201092.4 , XM_005201092.3
    Accession Version XM_005201092.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4698bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGACGCA GCCAGCTTCC ACCTCAAGCC GGACTGCCAG GCCCGGGACT TGCTGGACAC
    AGTGCAGCCA GACCGATTAT TCCGCCCCGT GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC
    GCACGGGTGT CTGCTGGGAG ACTGACTCCT GAAAGCCAAA GCAAAACCTC AGAAGTACTG
    AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT
    TCTCTTGCGC CACCTTCTCT TGCGCCACCT GTTCAGAAGA TGCAGGAGCC TCTCATCCCG
    GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG CAGCCCAGCC TGGAAACGCC CCCGCAGCCA
    CCCCCTCGGA GCAGGTCGTC CCACAGCTTG CCTTCTGAGC CTCCGGCGCA GCCGCAGGTG
    AAAACAAACG GAGTCTCCGC CGTCAGACTG GACTCGCCAT TAAAGAGTGA CCCATTTGAA
    GACTTGTCAT TGAACCTGCT TGCTGTATCA AAGGCTCAGC CATCTGTTCA CACCCCAAAC
    CCAAGGGGGT TGACGCCGTT GCCTTCTGCA GCCCCAAGTA ACACCAACAC TCTGAGTTCT
    GTAAGCTGCA TGCCGACAAT GCCTCCAATT CCAGCCCGGA GTAAATCCCA GGAAAACACG
    CGATGTTCCC CAAACCCATT CATCCCAAGC TCAAGCAGCA CAAATCCTTT CACCGACAGG
    ACCGCCGCTC CTGGAAACCC CTTTCGAGCT GAGTCTCAAG AATCAGAGGC CACTTCATGG
    TTCTCCAAAG AAGAGCCTGT TGCTCCGAGT CCATTCTCTT CGCTGAGGCC TCTGGATCAG
    AACAGTAGCA AGCCTTCATC CTCCCTGGAT GGGTTTAAGG ACAGTTTTGA TCCACAGGGC
    CTGTCTGCAC TAACAGTCAG CAACCCCAAA GGATGGGTAA CCTTCGAGGA AGAAGAGGAC
    TTTGGTGTGA CAGGGAAGTC AGGGTCCACT CGCCCAGACG TTTTCCTGGG TAAGCAGCTG
    AGCTCGTCTC CTGGCTCCAA GGTGATGCTT GGTGATGACT GGGGTAGGAG TACCAATGTG
    TCTCTCTGTG TGTTGCCAGC AAGACCACCA CCCCCACCGC CTGTCCCTCT GCTCCCACCT
    GGCACCACCC CTCCCGCAGA CGCTTTCACA GCCCTGGCCT CTAAGGCATC ACCCACGCTA
    GACTTGACAG AAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201149.2
    CDS29..4726
    Translation

    Target ORF information:

    RefSeq Version XM_005201092.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201092.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGCAT CTTCTAAAGT ACTAAAGAGT ATGTGTGAGA ATTTCTACAA GTATTCAAAG
    CCCAAGAAAA TTCGAGTATG TGTGGGCACA TGGAACGTGA ATGGCGGGAA GCAGTTTCGC
    AGCATAGCTT TTAAGAATCA GACCCTTACG GACTGGCTTC TTGATGCCCC CAAGTTAGCT
    GGCATCCAGG AGTTTCAAGA TAAAAGAAGT AAACCAATGG ATATATTTGC AATTGGTTTT
    GAGGAAATGG TAGAGCTGAA TGCTGGAAAC ATTGTGAATG CAAGCACAAC AAATCAGAAG
    CTCTGGGCTG CGGAACTTCA GAAGACCATC TCCAGAGACA ACAAGTATGT GCTGCTGGCC
    TCTGAGCAGT TGGTGGGCGT CTGTCTGTTT GTTTTTATCA GACCACAGCA CGCTCCCTTC
    ATCAGGGATG TTGCAGTTGA TACTGTGAAA ACTGGAATGG GAGGCGCAAC TGGAAATAAG
    GGAGCAGTTG CAATACGAAT GCTGTTCCAC ACCACAAGCC TCTGCTTTGT CTGCAGCCAC
    TTCGCTGCGG GACAATCCCA AGTCAAAGAA CGAAATGATG ATTTTGTAGA AATAGCGCGG
    AAGTTGAGTT TTCCAATGGG AAGGCTGCTC TTCTCCCATG ACTATGTGTT TTGGTGTGGC
    GATTTCAACT ACCGAATCGA TCTCCCTAAT GAGGAAGTGA AAGAGCTTAT CAGACAGCAA
    AACTGGGATT CTCTTATCGC AGGAGATCAG CTTATCAATC AGAAAAATGC TGGACAGATT
    TTTAGAGGAT TTTTAGAAGG AAAAGTGACC TTTGCTCCAA CGTATAAATA CGACTTGTTT
    TCTGATGACT ACGACACTAG TGAAAAGTGC CGCACCCCTG CATGGACAGA CCGTGTCCTC
    TGGAGAAGAC GGAAGTGGCC TTTTGATAGA TCAGCTGAAG ATTTAGATCT CCTAAATGCT
    AGTTTTCAAG ATGAAAGCAA AATCCTCTAC ACATGGACTC CTGGCACTTT GCTGCACTAC
    GGAAGGGCTG AGCTGAAGAC TTCTGACCAT AGGCCTGTTG TTGCCCTGAT CGATATTGAT
    ATATTTGAAG TTGAAGCTGA AGAGAGGCAA AACATTTATA AAGAAGTAAT TGCAGTTCAG
    GGTCCACCAG ATGGTACGGT GTTGGTCTCA ATCAAAAGCT CTTTACCAGA AAATAATTTT
    TTCAACGATG CTTTGATTGA TGAGCTTTTA CAGCAGTTTA CAAATTTCGG TGAAGTTATA
    CTCATAAGAT TTGTGGAAGA TAAAATGTGG GTTACGTTTT TAGAGGGAAG CTCTGCCTTG
    AATGTTCTGA ACCTGAATGG GAAAGAGTTA CTGGGTAGGA CAATAACAAT TACTTTAAAA
    AGTCCAGACT GGATCAAAAC TTTGGAAGAA GAAATGAGCT TAGAGAAAAT CAACGTCCCC
    TTGCCATCAT CAACCAGCTC CACCCTCCTA GGTGAAGACG CGGAGGTCAC GGCCGACTTC
    GATATGGAAG GTGATGTGGA TGACTATAGT GCTGAGGTAG AGGAGATCCT TCCTCAGCAT
    CTCCAGCCGT CTTCCAGTTC TGGCCTTGGC ACGTCCCCCG GTTCTTCACC CCGGACCAGT
    CCCTGCCAGT CACCTACCAT ATCGGAGGGG CCTGTGCCTT CCCTCCCAGT GAGACCAAGT
    CGAGCTCCGT CCAGAACGCC CGGGCCCCCT GCTTCACAAA GTTCTCCTGT TGACACTCTG
    CCGGCAACAC AGCTGCAGCA GAAAGATTCT TCCCAGACCC TGGAACCCAA GCGGCCTCCC
    CCTCCCCGCC CGGTTGCTCC TCCTGCACGT CCAGCTCCTC CACAACGACC ACCTCCGCCT
    TCAGGACGCA GCCAGCTTCC ACCTCAAGCC GGACTGCCAG GCCCGGGACT TGCTGGACAC
    AGTGCAGCCA GACCGATTAT TCCGCCCCGT GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC
    GCACGGGTGT CTGCTGGGAG ACTGACTCCT GAAAGCCAAA GCAAAACCTC AGAAGTACTG
    AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT
    TCTCTTGCGC CACCTTCTCT TGCGCCACCT GTTCAGAAGA TGCAGGAGCC TCTCATCCCG
    GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG CAGCCCAGCC TGGAAACGCC CCCGCAGCCA
    CCCCCTCGGA GCAGGTCGTC CCACAGCTTG CCTTCTGAGC CTCCGGCGCA GCCGCAGGTG
    AAAACAAACG GAGTCTCCGC CGTCAGACTG GACTCGCCAT TAAAGAGTGA CCCATTTGAA
    GACTTGTCAT TGAACCTGCT TGCTGTATCA AAGGCTCAGC CATCTGTTCA CACCCCAAAC
    CCAAGGGGGT TGACGCCGTT GCCTTCTGCA GCCCCAAGTA ACACCAACAC TCTGAGTTCT
    GTAAGCTGCA TGCCGACAAT GCCTCCAATT CCAGCCCGGA GTAAATCCCA GGAAAACACG
    CGATGTTCCC CAAACCCATT CATCCCAAGC TCAAGCAGCA CAAATCCTTT CACCGACAGG
    ACCGCCGCTC CTGGAAACCC CTTTCGAGCT GAGTCTCAAG AATCAGAGGC CACTTCATGG
    TTCTCCAAAG AAGAGCCTGT TGCTCCGAGT CCATTCTCTT CGCTGAGGCC TCTGGATCAG
    AACAGTAGCA AGCCTTCATC CTCCCTGGAT GGGTTTAAGG ACAGTTTTGA TCCACAGGGC
    CTGTCTGCAC TAACAGTCAG CAACCCCAAA GGATGGGTAA CCTTCGAGGA AGAAGAGGAC
    TTTGGTGTGA CAGGGAAGTC AGGGTCCACT CGCCCAGACG TTTTCCTGGG TAAGCAGCTG
    AGCTCGTCTC CTGGCTCCAA GGTGATGCTT GGTGATGACT GGGGTAGGAG TACCAATGTG
    TCTCTCTGTG TGTTGCCAGC AAGACCACCA CCCCCACCGC CTGTCCCTCT GCTCCCACCT
    GGCACCACCC CTCCCGCAGA CGCTTTCACA GCCCTGGCCT CTAAGGCATC ACCCACGCTA
    GACTTGACAG AAAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45334
    Clone ID Related Accession (Same CDS sequence) XM_005201093.4 , XM_005201093.3
    Accession Version XM_005201093.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201093.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGCAG GAGCAACCAT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201150.2
    CDS16..4083
    Translation

    Target ORF information:

    RefSeq Version XM_005201093.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201093.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGCAG GAGCAACCAT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45334
    Clone ID Related Accession (Same CDS sequence) XM_005201093.4 , XM_005201093.3
    Accession Version XM_005201093.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201093.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGCAG GAGCAACCAT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201150.2
    CDS29..4096
    Translation

    Target ORF information:

    RefSeq Version XM_005201093.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201093.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGCAG GAGCAACCAT CAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45335
    Clone ID Related Accession (Same CDS sequence) XM_005201094.3 , XM_005201094.4
    Accession Version XM_005201094.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201094.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGGAG CAACCATCAG GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201151.2
    CDS29..4093
    Translation

    Target ORF information:

    RefSeq Version XM_005201094.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201094.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGGAG CAACCATCAG GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45335
    Clone ID Related Accession (Same CDS sequence) XM_005201094.3 , XM_005201094.4
    Accession Version XM_005201094.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201094.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGGAG CAACCATCAG GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201151.2
    CDS16..4080
    Translation

    Target ORF information:

    RefSeq Version XM_005201094.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201094.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGGTG TTGGAGCCCC TCCCAGTCCG GGGGTAGCTA GGCGAGAGAT GGAAGCACCC
    AAAAGCCCAG GAACGACCCG GAGAGATAAT CTAGGACGCA GCCAGCTTCC ACCTCAAGCC
    GGACTGCCAG GCCCGGGACT TGCTGGACAC AGTGCAGCCA GACCGATTAT TCCGCCCCGT
    GCTGGAGTCA TCAGCGCCCC CCAGAGCCAC GCACGGGTGT CTGCTGGGAG ACTGACTCCT
    GAAAGCCAAA GCAAAACCTC AGAAGTACTG AAAGGGCCAG CTCTTCTTCC CGAGCCCCTG
    AAGCCTCAGG CCGCCCTTCC TGTGCCGCCT TCTCTTGCGC CACCTTCTCT TGCGCCACCT
    GTTCAGAAGA TGCAGGAGCC TCTCATCCCG GTGGCCGCAC CTCTGGCCCA GGCTGCCCTG
    CAGCCCAGCC TGGAAACGCC CCCGCAGCCA CCCCCTCGGA GCAGGTCGTC CCACAGCTTG
    CCTTCTGAGC CTCCGGCGCA GCCGCAGGAG CAACCATCAG GGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45336
    Clone ID Related Accession (Same CDS sequence) XM_005201095.3 , XM_005201095.4
    Accession Version XM_005201095.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4020bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGCAC CCAAAAGCCC AGGAACGACC CGGAGAGATA ATCTAGGACG CAGCCAGCTT
    CCACCTCAAG CCGGACTGCC AGGCCCGGGA CTTGCTGGAC ACAGTGCAGC CAGACCGATT
    ATTCCGCCCC GTGCTGGAGT CATCAGCGCC CCCCAGAGCC ACGCACGGGT GTCTGCTGGG
    AGACTGACTC CTGAAAGCCA AAGCAAAACC TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT
    CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT CCTGTGCCGC CTTCTCTTGC GCCACCTTCT
    CTTGCGCCAC CTGTTCAGAA GATGCAGGAG CCTCTCATCC CGGTGGCCGC ACCTCTGGCC
    CAGGCTGCCC TGCAGCCCAG CCTGGAAACG CCCCCGCAGC CACCCCCTCG GAGCAGGTCG
    TCCCACAGCT TGCCTTCTGA GCCTCCGGCG CAGCCGCAGC AGGAGCAACC ATCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201152.2
    CDS29..4048
    Translation

    Target ORF information:

    RefSeq Version XM_005201095.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201095.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGCAC CCAAAAGCCC AGGAACGACC CGGAGAGATA ATCTAGGACG CAGCCAGCTT
    CCACCTCAAG CCGGACTGCC AGGCCCGGGA CTTGCTGGAC ACAGTGCAGC CAGACCGATT
    ATTCCGCCCC GTGCTGGAGT CATCAGCGCC CCCCAGAGCC ACGCACGGGT GTCTGCTGGG
    AGACTGACTC CTGAAAGCCA AAGCAAAACC TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT
    CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT CCTGTGCCGC CTTCTCTTGC GCCACCTTCT
    CTTGCGCCAC CTGTTCAGAA GATGCAGGAG CCTCTCATCC CGGTGGCCGC ACCTCTGGCC
    CAGGCTGCCC TGCAGCCCAG CCTGGAAACG CCCCCGCAGC CACCCCCTCG GAGCAGGTCG
    TCCCACAGCT TGCCTTCTGA GCCTCCGGCG CAGCCGCAGC AGGAGCAACC ATCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45336
    Clone ID Related Accession (Same CDS sequence) XM_005201095.3 , XM_005201095.4
    Accession Version XM_005201095.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4020bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201095.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGCAC CCAAAAGCCC AGGAACGACC CGGAGAGATA ATCTAGGACG CAGCCAGCTT
    CCACCTCAAG CCGGACTGCC AGGCCCGGGA CTTGCTGGAC ACAGTGCAGC CAGACCGATT
    ATTCCGCCCC GTGCTGGAGT CATCAGCGCC CCCCAGAGCC ACGCACGGGT GTCTGCTGGG
    AGACTGACTC CTGAAAGCCA AAGCAAAACC TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT
    CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT CCTGTGCCGC CTTCTCTTGC GCCACCTTCT
    CTTGCGCCAC CTGTTCAGAA GATGCAGGAG CCTCTCATCC CGGTGGCCGC ACCTCTGGCC
    CAGGCTGCCC TGCAGCCCAG CCTGGAAACG CCCCCGCAGC CACCCCCTCG GAGCAGGTCG
    TCCCACAGCT TGCCTTCTGA GCCTCCGGCG CAGCCGCAGC AGGAGCAACC ATCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201152.2
    CDS16..4035
    Translation

    Target ORF information:

    RefSeq Version XM_005201095.4
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201095.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GGCTGGAAAG TTAAAAGATG GTGCTCGTTC AGTTAGTAGA
    ACAATTCAGA ATAACTTCTT TGACAGCTCC AAGCAAGAAG CAATTGATGT TTTGCTCCTG
    GGAAATACTC TAAATAGTGA TTTAGCTGAC AAAGCTCGAG CCCTTTTAAC TACTGGAAGT
    TTGCGTGTTT CTGAACAGAC GTTACAGTCA GCATCTTCTA AAGTACTAAA GAGTATGTGT
    GAGAATTTCT ACAAGTATTC AAAGCCCAAG AAAATTCGAG TATGTGTGGG CACATGGAAC
    GTGAATGGCG GGAAGCAGTT TCGCAGCATA GCTTTTAAGA ATCAGACCCT TACGGACTGG
    CTTCTTGATG CCCCCAAGTT AGCTGGCATC CAGGAGTTTC AAGATAAAAG AAGTAAACCA
    ATGGATATAT TTGCAATTGG TTTTGAGGAA ATGGTAGAGC TGAATGCTGG AAACATTGTG
    AATGCAAGCA CAACAAATCA GAAGCTCTGG GCTGCGGAAC TTCAGAAGAC CATCTCCAGA
    GACAACAAGT ATGTGCTGCT GGCCTCTGAG CAGTTGGTGG GCGTCTGTCT GTTTGTTTTT
    ATCAGACCAC AGCACGCTCC CTTCATCAGG GATGTTGCAG TTGATACTGT GAAAACTGGA
    ATGGGAGGCG CAACTGGAAA TAAGGGAGCA GTTGCAATAC GAATGCTGTT CCACACCACA
    AGCCTCTGCT TTGTCTGCAG CCACTTCGCT GCGGGACAAT CCCAAGTCAA AGAACGAAAT
    GATGATTTTG TAGAAATAGC GCGGAAGTTG AGTTTTCCAA TGGGAAGGCT GCTCTTCTCC
    CATGACTATG TGTTTTGGTG TGGCGATTTC AACTACCGAA TCGATCTCCC TAATGAGGAA
    GTGAAAGAGC TTATCAGACA GCAAAACTGG GATTCTCTTA TCGCAGGAGA TCAGCTTATC
    AATCAGAAAA ATGCTGGACA GATTTTTAGA GGATTTTTAG AAGGAAAAGT GACCTTTGCT
    CCAACGTATA AATACGACTT GTTTTCTGAT GACTACGACA CTAGTGAAAA GTGCCGCACC
    CCTGCATGGA CAGACCGTGT CCTCTGGAGA AGACGGAAGT GGCCTTTTGA TAGATCAGCT
    GAAGATTTAG ATCTCCTAAA TGCTAGTTTT CAAGATGAAA GCAAAATCCT CTACACATGG
    ACTCCTGGCA CTTTGCTGCA CTACGGAAGG GCTGAGCTGA AGACTTCTGA CCATAGGCCT
    GTTGTTGCCC TGATCGATAT TGATATATTT GAAGTTGAAG CTGAAGAGAG GCAAAACATT
    TATAAAGAAG TAATTGCAGT TCAGGGTCCA CCAGATGGTA CGGTGTTGGT CTCAATCAAA
    AGCTCTTTAC CAGAAAATAA TTTTTTCAAC GATGCTTTGA TTGATGAGCT TTTACAGCAG
    TTTACAAATT TCGGTGAAGT TATACTCATA AGATTTGTGG AAGATAAAAT GTGGGTTACG
    TTTTTAGAGG GAAGCTCTGC CTTGAATGTT CTGAACCTGA ATGGGAAAGA GTTACTGGGT
    AGGACAATAA CAATTACTTT AAAAAGTCCA GACTGGATCA AAACTTTGGA AGAAGAAATG
    AGCTTAGAGA AAATCAACGT CCCCTTGCCA TCATCAACCA GCTCCACCCT CCTAGGTGAA
    GACGCGGAGG TCACGGCCGA CTTCGATATG GAAGGTGATG TGGATGACTA TAGTGCTGAG
    GTAGAGGAGA TCCTTCCTCA GCATCTCCAG CCGTCTTCCA GTTCTGGCCT TGGCACGTCC
    CCCGGTTCTT CACCCCGGAC CAGTCCCTGC CAGTCACCTA CCATATCGGA GGGGCCTGTG
    CCTTCCCTCC CAGTGAGACC AAGTCGAGCT CCGTCCAGAA CGCCCGGGCC CCCTGCTTCA
    CAAAGTTCTC CTGTTGACAC TCTGCCGGCA ACACAGCTGC AGCAGAAAGA TTCTTCCCAG
    ACCCTGGAAC CCAAGCGGCC TCCCCCTCCC CGCCCGGTTG CTCCTCCTGC ACGTCCAGCT
    CCTCCACAAC GACCACCTCC GCCTTCAGGG GCTAGGAGTC CTGCACCTGC TAGAAAAGAG
    TTTGGAGCAC CCAAAAGCCC AGGAACGACC CGGAGAGATA ATCTAGGACG CAGCCAGCTT
    CCACCTCAAG CCGGACTGCC AGGCCCGGGA CTTGCTGGAC ACAGTGCAGC CAGACCGATT
    ATTCCGCCCC GTGCTGGAGT CATCAGCGCC CCCCAGAGCC ACGCACGGGT GTCTGCTGGG
    AGACTGACTC CTGAAAGCCA AAGCAAAACC TCAGAAGTAC TGAAAGGGCC AGCTCTTCTT
    CCCGAGCCCC TGAAGCCTCA GGCCGCCCTT CCTGTGCCGC CTTCTCTTGC GCCACCTTCT
    CTTGCGCCAC CTGTTCAGAA GATGCAGGAG CCTCTCATCC CGGTGGCCGC ACCTCTGGCC
    CAGGCTGCCC TGCAGCCCAG CCTGGAAACG CCCCCGCAGC CACCCCCTCG GAGCAGGTCG
    TCCCACAGCT TGCCTTCTGA GCCTCCGGCG CAGCCGCAGC AGGAGCAACC ATCAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45337
    Clone ID Related Accession (Same CDS sequence) XM_005201096.3
    Accession Version XM_005201096.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product synaptojanin-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103793.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GTTAAAAGAT GGTGCTCGTT CAGTTAGTAG AACAATTCAG
    AATAACTTCT TTGACAGCTC CAAGCAAGAA GCAATTGATG TTTTGCTCCT GGGAAATACT
    CTAAATAGTG ATTTAGCTGA CAAAGCTCGA GCCCTTTTAA CTACTGGAAG TTTGCGTGTT
    TCTGAACAGA CGTTACAGTC AGCATCTTCT AAAGTACTAA AGAGTATGTG TGAGAATTTC
    TACAAGTATT CAAAGCCCAA GAAAATTCGA GTATGTGTGG GCACATGGAA CGTGAATGGC
    GGGAAGCAGT TTCGCAGCAT AGCTTTTAAG AATCAGACCC TTACGGACTG GCTTCTTGAT
    GCCCCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA GAAGTAAACC AATGGATATA
    TTTGCAATTG GTTTTGAGGA AATGGTAGAG CTGAATGCTG GAAACATTGT GAATGCAAGC
    ACAACAAATC AGAAGCTCTG GGCTGCGGAA CTTCAGAAGA CCATCTCCAG AGACAACAAG
    TATGTGCTGC TGGCCTCTGA GCAGTTGGTG GGCGTCTGTC TGTTTGTTTT TATCAGACCA
    CAGCACGCTC CCTTCATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG AATGGGAGGC
    GCAACTGGAA ATAAGGGAGC AGTTGCAATA CGAATGCTGT TCCACACCAC AAGCCTCTGC
    TTTGTCTGCA GCCACTTCGC TGCGGGACAA TCCCAAGTCA AAGAACGAAA TGATGATTTT
    GTAGAAATAG CGCGGAAGTT GAGTTTTCCA ATGGGAAGGC TGCTCTTCTC CCATGACTAT
    GTGTTTTGGT GTGGCGATTT CAACTACCGA ATCGATCTCC CTAATGAGGA AGTGAAAGAG
    CTTATCAGAC AGCAAAACTG GGATTCTCTT ATCGCAGGAG ATCAGCTTAT CAATCAGAAA
    AATGCTGGAC AGATTTTTAG AGGATTTTTA GAAGGAAAAG TGACCTTTGC TCCAACGTAT
    AAATACGACT TGTTTTCTGA TGACTACGAC ACTAGTGAAA AGTGCCGCAC CCCTGCATGG
    ACAGACCGTG TCCTCTGGAG AAGACGGAAG TGGCCTTTTG ATAGATCAGC TGAAGATTTA
    GATCTCCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATCC TCTACACATG GACTCCTGGC
    ACTTTGCTGC ACTACGGAAG GGCTGAGCTG AAGACTTCTG ACCATAGGCC TGTTGTTGCC
    CTGATCGATA TTGATATATT TGAAGTTGAA GCTGAAGAGA GGCAAAACAT TTATAAAGAA
    GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACGGTGTTGG TCTCAATCAA AAGCTCTTTA
    CCAGAAAATA ATTTTTTCAA CGATGCTTTG ATTGATGAGC TTTTACAGCA GTTTACAAAT
    TTCGGTGAAG TTATACTCAT AAGATTTGTG GAAGATAAAA TGTGGGTTAC GTTTTTAGAG
    GGAAGCTCTG CCTTGAATGT TCTGAACCTG AATGGGAAAG AGTTACTGGG TAGGACAATA
    ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG AAGAAGAAAT GAGCTTAGAG
    AAAATCAACG TCCCCTTGCC ATCATCAACC AGCTCCACCC TCCTAGGTGA AGACGCGGAG
    GTCACGGCCG ACTTCGATAT GGAAGGTGAT GTGGATGACT ATAGTGCTGA GGTAGAGGAG
    ATCCTTCCTC AGCATCTCCA GCCGTCTTCC AGTTCTGGCC TTGGCACGTC CCCCGGTTCT
    TCACCCCGGA CCAGTCCCTG CCAGTCACCT ACCATATCGG AGGGGCCTGT GCCTTCCCTC
    CCAGTGAGAC CAAGTCGAGC TCCGTCCAGA ACGCCCGGGC CCCCTGCTTC ACAAAGTTCT
    CCTGTTGACA CTCTGCCGGC AACACAGCTG CAGCAGAAAG ATTCTTCCCA GACCCTGGAA
    CCCAAGCGGC CTCCCCCTCC CCGCCCGGTT GCTCCTCCTG CACGTCCAGC TCCTCCACAA
    CGACCACCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCTG CTAGAAAAGA GTTTGGAGCA
    CCCAAAAGCC CAGGAACGAC CCGGAGAGAT AATCTAGGAC GCAGCCAGCT TCCACCTCAA
    GCCGGACTGC CAGGCCCGGG ACTTGCTGGA CACAGTGCAG CCAGACCGAT TATTCCGCCC
    CGTGCTGGAG TCATCAGCGC CCCCCAGAGC CACGCACGGG TGTCTGCTGG GAGACTGACT
    CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC
    CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG CCTTCTCTTG CGCCACCTTC TCTTGCGCCA
    CCTGTTCAGA AGATGCAGGA GCCTCTCATC CCGGTGGCCG CACCTCTGGC CCAGGCTGCC
    CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG CCACCCCCTC GGAGCAGGTC GTCCCACAGC
    TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG CAGGAGCAAC CATCAGGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201153.2
    CDS29..4039
    Translation

    Target ORF information:

    RefSeq Version XM_005201096.3
    Organism Bos taurus(cattle)
    Definition Bos taurus synaptojanin 1 (SYNJ1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGGAAGA GATGGACGCG CCGGAGCGGA AGTGACGGTT GCGGCGGCGG CGGCCGCGGA 
    AGGAGGAGGA GGAGCCGGAG GAAGAGGGCT GCCTCCGAAG AAAGGAGAAT GGCGTTCAGC
    AAAGGATTTC GGATCTATCA CAAATTGGAT CCCCCACCTT TCAGCCTCAT AGTGGAAACC
    AGGCATAAGG AAGAATGTCT CATGTTCGAG TCTGGGGCTG TAGCGGTGCT CTCATCAGCA
    GAAAAAGAGG CAATCAAGGG TACATACTCC AAAGTTCTAG ATGCCTATGG ACTTTTAGGT
    GTTTTACGAT TAAATCTTGG TGATATTATG TTACATTATC TGGTCCTAGT CACTGGATGT
    ATGTCTGTTG GAAAAATTCA AGAATCTGAA GTTTTCCGAG TTACTTCCAC TGAATTTATA
    TCACTGCGAG TTGATTCTTC GGATGAGGAT CGCATTTCGG AAGTGCGAAA AGTTTTAAAT
    TCAGGAAACT TTTATTTTGC ATGGTCTGCA TCCGGAGTCA GTTTGGATCT GAGTCTTAAT
    GCCCACCGTA GCTTGCAAGA ACACACAACT GACAATAGAT TTTTCTGGAA TCAGTCTTTG
    CACTTGCATC TCAAGCACTA CGGTGTGAAT TGTGACGACT GGTTATTACG CCTCATGTGT
    GGGGGAGTAG AAATCAGAAC AATTTATGCT GCTCATAAAC AGGCAAAGGC TTGCCTCATT
    TCCAGACTAA GCTGTGAACG AGCTGGGACC AGGTTTAACG TCCGGGGAAC AAATGATGAT
    GGTCACGTTG CCAATTTTGT AGAAACAGAA CAGGTTGTGT ACTTAGATGA CTCTGTTTCT
    TCCTTCATAC AAATCCGGGG ATCTGTCCCA TTGTTCTGGG AGCAGCCAGG ATTGCAGGTG
    GGATCTCATC GTGTCCGTAT GTCAAGGGGA TTTGAAGCCA ATGCACCTGC TTTTGACAGG
    CATTTTAGGA CACTTAAGAA TTTGTATGGT AAACAAATAA TAGTAAATCT GCTTGGATCT
    AAGGAAGGTG AACATATGCT AAGTAAGGCT TTCCAGAGTC ATCTGAAAGC TTCTGAACAT
    GCTGCTGATA TCCAGATGGT GAATTTTGAC TATCATCAAA TGGTTAAAGG AGGAAAGGCA
    GAAAAATTAC ATAGTGTTCT TAAACCTCAA GTCCAGAAGT TTCTGGATTA TGGAATTTTT
    CATTTTGATG GAAGTGAAGT TCAAAGGTGC CAGAGTGGTA CAGTTCGAAC AAACTGCTTG
    GATTGTCTTG ACAGAACAAA TAGTGTGCAG GCATTCCTTG GTTTAGAGAT GCTGACTAAA
    CAGTTGGAAG CTCTTGGTTT AGCTGAAAAG CCTCAGTTGG TGACTCGCTT TCAAGAAGTT
    TTTCGATCAA TGTGGTCTGT GAATGGTGAT TCAATCAGTA AGATTTATGC AGGAACTGGA
    GCCCTTGAAG GAAAGGCTAA GTTAAAAGAT GGTGCTCGTT CAGTTAGTAG AACAATTCAG
    AATAACTTCT TTGACAGCTC CAAGCAAGAA GCAATTGATG TTTTGCTCCT GGGAAATACT
    CTAAATAGTG ATTTAGCTGA CAAAGCTCGA GCCCTTTTAA CTACTGGAAG TTTGCGTGTT
    TCTGAACAGA CGTTACAGTC AGCATCTTCT AAAGTACTAA AGAGTATGTG TGAGAATTTC
    TACAAGTATT CAAAGCCCAA GAAAATTCGA GTATGTGTGG GCACATGGAA CGTGAATGGC
    GGGAAGCAGT TTCGCAGCAT AGCTTTTAAG AATCAGACCC TTACGGACTG GCTTCTTGAT
    GCCCCCAAGT TAGCTGGCAT CCAGGAGTTT CAAGATAAAA GAAGTAAACC AATGGATATA
    TTTGCAATTG GTTTTGAGGA AATGGTAGAG CTGAATGCTG GAAACATTGT GAATGCAAGC
    ACAACAAATC AGAAGCTCTG GGCTGCGGAA CTTCAGAAGA CCATCTCCAG AGACAACAAG
    TATGTGCTGC TGGCCTCTGA GCAGTTGGTG GGCGTCTGTC TGTTTGTTTT TATCAGACCA
    CAGCACGCTC CCTTCATCAG GGATGTTGCA GTTGATACTG TGAAAACTGG AATGGGAGGC
    GCAACTGGAA ATAAGGGAGC AGTTGCAATA CGAATGCTGT TCCACACCAC AAGCCTCTGC
    TTTGTCTGCA GCCACTTCGC TGCGGGACAA TCCCAAGTCA AAGAACGAAA TGATGATTTT
    GTAGAAATAG CGCGGAAGTT GAGTTTTCCA ATGGGAAGGC TGCTCTTCTC CCATGACTAT
    GTGTTTTGGT GTGGCGATTT CAACTACCGA ATCGATCTCC CTAATGAGGA AGTGAAAGAG
    CTTATCAGAC AGCAAAACTG GGATTCTCTT ATCGCAGGAG ATCAGCTTAT CAATCAGAAA
    AATGCTGGAC AGATTTTTAG AGGATTTTTA GAAGGAAAAG TGACCTTTGC TCCAACGTAT
    AAATACGACT TGTTTTCTGA TGACTACGAC ACTAGTGAAA AGTGCCGCAC CCCTGCATGG
    ACAGACCGTG TCCTCTGGAG AAGACGGAAG TGGCCTTTTG ATAGATCAGC TGAAGATTTA
    GATCTCCTAA ATGCTAGTTT TCAAGATGAA AGCAAAATCC TCTACACATG GACTCCTGGC
    ACTTTGCTGC ACTACGGAAG GGCTGAGCTG AAGACTTCTG ACCATAGGCC TGTTGTTGCC
    CTGATCGATA TTGATATATT TGAAGTTGAA GCTGAAGAGA GGCAAAACAT TTATAAAGAA
    GTAATTGCAG TTCAGGGTCC ACCAGATGGT ACGGTGTTGG TCTCAATCAA AAGCTCTTTA
    CCAGAAAATA ATTTTTTCAA CGATGCTTTG ATTGATGAGC TTTTACAGCA GTTTACAAAT
    TTCGGTGAAG TTATACTCAT AAGATTTGTG GAAGATAAAA TGTGGGTTAC GTTTTTAGAG
    GGAAGCTCTG CCTTGAATGT TCTGAACCTG AATGGGAAAG AGTTACTGGG TAGGACAATA
    ACAATTACTT TAAAAAGTCC AGACTGGATC AAAACTTTGG AAGAAGAAAT GAGCTTAGAG
    AAAATCAACG TCCCCTTGCC ATCATCAACC AGCTCCACCC TCCTAGGTGA AGACGCGGAG
    GTCACGGCCG ACTTCGATAT GGAAGGTGAT GTGGATGACT ATAGTGCTGA GGTAGAGGAG
    ATCCTTCCTC AGCATCTCCA GCCGTCTTCC AGTTCTGGCC TTGGCACGTC CCCCGGTTCT
    TCACCCCGGA CCAGTCCCTG CCAGTCACCT ACCATATCGG AGGGGCCTGT GCCTTCCCTC
    CCAGTGAGAC CAAGTCGAGC TCCGTCCAGA ACGCCCGGGC CCCCTGCTTC ACAAAGTTCT
    CCTGTTGACA CTCTGCCGGC AACACAGCTG CAGCAGAAAG ATTCTTCCCA GACCCTGGAA
    CCCAAGCGGC CTCCCCCTCC CCGCCCGGTT GCTCCTCCTG CACGTCCAGC TCCTCCACAA
    CGACCACCTC CGCCTTCAGG GGCTAGGAGT CCTGCACCTG CTAGAAAAGA GTTTGGAGCA
    CCCAAAAGCC CAGGAACGAC CCGGAGAGAT AATCTAGGAC GCAGCCAGCT TCCACCTCAA
    GCCGGACTGC CAGGCCCGGG ACTTGCTGGA CACAGTGCAG CCAGACCGAT TATTCCGCCC
    CGTGCTGGAG TCATCAGCGC CCCCCAGAGC CACGCACGGG TGTCTGCTGG GAGACTGACT
    CCTGAAAGCC AAAGCAAAAC CTCAGAAGTA CTGAAAGGGC CAGCTCTTCT TCCCGAGCCC
    CTGAAGCCTC AGGCCGCCCT TCCTGTGCCG CCTTCTCTTG CGCCACCTTC TCTTGCGCCA
    CCTGTTCAGA AGATGCAGGA GCCTCTCATC CCGGTGGCCG CACCTCTGGC CCAGGCTGCC
    CTGCAGCCCA GCCTGGAAAC GCCCCCGCAG CCACCCCCTC GGAGCAGGTC GTCCCACAGC
    TTGCCTTCTG AGCCTCCGGC GCAGCCGCAG CAGGAGCAAC CATCAGGGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.