SCN5A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCN5A
Entrez Gene ID	282061
Full Name	sodium voltage-gated channel alpha subunit 5
General protein information	Preferred Names sodium voltage-gated channel alpha subunit 5 Names sodium channel protein type 5 subunit alpha sodium channel, voltage-gated, type V, alpha polypeptide (long (electrocardiographic) QT syndrome 3) sodium channel, voltage-gated, type V, alpha subunit voltage-gated sodium channel type V alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174458.2	NP_776883.1	sodium channel protein type 5 subunit alpha
XM_024982395.1	XP_024838163.1	sodium channel protein type 5 subunit alpha isoform X4
XM_024982392.1	XP_024838160.1	sodium channel protein type 5 subunit alpha isoform X1
XM_024982394.1	XP_024838162.1	sodium channel protein type 5 subunit alpha isoform X3
XM_024982397.1	XP_024838165.1	sodium channel protein type 5 subunit alpha isoform X6
XM_024982393.1	XP_024838161.1	sodium channel protein type 5 subunit alpha isoform X2
XM_024982396.1	XP_024838164.1	sodium channel protein type 5 subunit alpha isoform X5

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	scn12ab	NP_001038588.1
Homo sapiens (human)	SCN5A	NP_932173.1
Pan troglodytes (chimpanzee)	SCN5A	XP_001171891.2
	LOC695085	XP_002808375.1
Canis lupus familiaris (dog)	SCN5A	NP_001002994.1
Danio rerio (zebrafish)	scn12aa	NP_001038387.1
Rattus norvegicus (Norway rat)	Scn5a	NP_037257.1
Bos taurus (cattle)	SCN5A	NP_776883.1
Mus musculus (house mouse)	Scn5a	NP_067519.2
Gallus gallus (chicken)	SCN5A	XP_001232818.3
Xenopus tropicalis (tropical clawed frog)	scn5a	XP_002932534.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SCN5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCN5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa228732	XM_024982395.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228733	XM_024982396.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228734	XM_024982397.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228729	XM_024982392.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa228730	XM_024982393.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa228731	XM_024982394.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa40952	NM_174458.2 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCN5A ( XM_024982395.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838163.1 Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa228732
Clone ID Related Accession (Same CDS sequence)	XM_024982395.1
Accession Version	XM_024982395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 5 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CATACACCAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC TTTACGTACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCAGTCATTT CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGCTTCCCG AAGACAGTTA CTCTGAGGGG AGCACGGCAG ATATGACCAA CACTGCTGAC 
CTCCTGGAGC AGATCCCTGA CCTCGGAGAG GATGTCAAAG ATCCAGAGGA CTGCTTCACT 
GAAGGCTGTG TCCGCCGCTG TCCCTGCTGC ACCGTGGACA CCACACAGGC CCACGGGAAG 
GTCTGGTGGA GGCTGCGCAA GACCTGCTAC CGCATCGTGG AGCACAGCTG GTTCGAGACG 
TTCATCATCT TCATGATCCT GCTCAGCAGT GGCGCACTGG CCTTTGAGGA CATCTACCTG 
GAGGAACGGA AGACCATCAA GGTCCTGCTG GAGTACGCCG ACAAGATGTT CACCTACGTC 
TTCGTGCTGG AGATGCTCCT CAAGTGGGTG GCCTACGGCT TCAAGAAGTA CTTCACCAAC 
GCCTGGTGCT GGCTTGATTT CCTCATCGTG GACGTCTCGC TGATCAGCCT GGTGGCCAAC 
GCCCTGGGCT TTGCTGAGAT GGGCCCCATC AAGTCACTGC GGACCTTGCG TGCGCTCAGA 
CCCCTGCGAG CCCTGTCACG ATTTGAGGGC ATGAGGGTTG TGGTTAACGC CCTGGTGGGC 
GCCATCCCAT CCATCATGAA CGTCCTCCTC GTCTGCCTCA TCTTCTGGCT CATCTTCAGC 
ATCATGGGCG TGAACCTCTT CGCGGGGAAG TTTGGGAGAT GCATCAACCA GACCGAGGGA 
GACCTGCCCT TGAACTATAC CATCGTGAAC AACAAGAGCG ACTGTGAGTC TTTCAACGTG 
ACTGGCGAAT TGTACTGGAC CAAGGTGAAG GTCAACTTTG ACAACGTGGG GGCCGGGTAC 
CTGGCCCTTC TGCAGGTGGC AACATTTAAA GGCTGGATGG ACATCATGTA TGCAGCTGTA 
GACTCCAGGG GGTACGAGGA GCAGCCCCAG TGGGAATACA ACCTCTACAT GTATATCTAT 
TTTGTCATCT TCATCATCTT TGGGTCTTTC TTCACCCTGA ACCTGTTCAT CGGTGTCATC 
ATTGACAACT TCAACCAGCA GAAGAAAAAG TTAGGGGGCC AGGACATCTT CATGACAGAG 
GAGCAGAAGA AGTACTACAA CGCCATGAAG AAGCTGGGCT CCAAGAAGCC CCAGAAGCCC 
ATCCCACGGC CCCTGAACAA GTACCAGGGC TTCATATTCG ACATTGTGAC CAAGCAGGCC 
TTCGACGTCA CCATCATGTT TCTCATCTGC TTAAACATGG TGACCATGAT GGTGGAGACA 
GACGACCAGA GCCCCGAGAA GGTCAACATC TTGGCCAAGA TCAACCTGCT GTTCGTGGGC 
ATCTTCACAG CCGAGTGTAT CTTCAAGATG GTTGCCCTGC GCCACTATTA CTTCACCAAC 
AGCTGGAACA TCTTCGACTT CGTGGTTGTC ATCCTCTCCA TCGTAGGCAC TGTGCTCTCA 
GACATCATCC AGAAGTACTT CTTCTCCCCG ACGCTCTTCC GCGTCATCCG CCTGGCCCGC 
ATAGGCCGCA TCCTCAGGCT GATCCGCGGG GCCAAGGGCA TCCGCACGCT GCTCTTCGCC 
CTCATGATGT CCCTGCCCGC GCTCTTCAAC ATCGGGCTGC TGCTCTTCCT CGTCATGTTC 
ATCTACTCCA TCTTCGGCAT GGCCAACTTC GCCTACGTCA AGTGGGAGGC TGGCATCGAC 
GACATGTTCA ACTTCCAGAC CTTCGCCAAC AGCATGCTGT GCCTCTTCCA GATCACCACG 
TCGGCGGGCT GGGATGGGCT CCTCAGCCCC ATCCTCAACA CGGGGCCCCC CTACTGTGAC 
CCCAACCTGC CCAACAGCAA CGGCTCCCGG GGCAACTGCG GGAGCCCTGC GGTGGGCATC 
CTCTTCTTCA CCACCTACAT CATCATCTCC TTCCTCATTG TGGTCAACAT GTACATCGCC 
ATCATCCTGG AGAACTTCAG CGTGGCCACG GAGGAGAGCA CGGAGCCCCT GAGTGAGGAT 
GACTTCGACA TGTTCTACGA GATCTGGGAG AAGTTCGACC CGGAGGCCAC CCAGTTCATC 
GAGTATTTGG CCCTGTCTGA CTTCGCCGAT GCCCTGTCAG AGCCACTCCG GATCCCCAAG 
CCCAACCAGA TAAGCCTCAT CAATATGGAC CTGCCCATGG TGAGTGGAGA CCGCATCCAC 
TGCATGGACA TCCTCTTTGC CTTCACCAAG AGGGTCCTGG GCGAATCTGG GGAGATGGAC 
GCCCTGAAGA TCCAGATGGA GGAGAAGTTC ATGGCGGCCA ACCCGTCCAA GATCTCCTAC 
GAGCCCATCA CCACCACGCT GCGGCGGAAG CACGAGGAGG TGTCGGCCAC GATCATCCAG 
CGGGCCTTCC GCCGGCACCT GCTGCAGCGC TCCGTCAAGC ACGCCTCCTT CCTCTACCGC 
CAGCAGGCGG GCAGCAGCGG CCTCTCGGAG GAGGACGCCC CCGAGCAGGA GGGCCTCATC 
GCCTACATGA TGAACGAGAA CTTCTCCCGC CGCCCCGGCC CGCCCTCCAG CTCCTCCGTC 
TCCTCCACGT CCTTCCCGCC CTCCTATGAC AGCGTCACCA GGGCCACCAG CGACAACCCC 
CAGGTGCGGG CGTCTGACTA CAGCCCAAGC GAGGATCTCG CAGACTTCCC CCCAACCCCC 
GACAGGGACC GTGAGTCAAT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838163.1
CDS	1..5907
Translation	MAAFLLPRGTSSFRRFTRESLAAIEKRMAEKQARSSAASQESRDGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALHVLSPFHPIRRVAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYIFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTVLNGTNSTNASVEADGLIWASLDDYLNDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMELLKKEQEALAIRGVDTVSRSSLEMSPLAPVTTHERRSKRRKRMSSGMEECGDDKFPKSDSEDGPRAVNRFSITHGLSRTSMKPRSSHGSIFTFRRRDLGSETDFADDENSTAGDSESHRTSLLVPWPLRRPSTLGQPSPGTSTPGHVLNGKRNSTVDCNGVVSLLGAGDPEATSPGSHLLHPMKLERPPDTTTPSEEPGRPQTLTPQAPCVDGFEEPGERQRALSAVSVLTSALEELEESQRRCPPCWIRFAQHYLIWECCPLWMSIKQKVKFMVMDPFADLTITMCIVLNTLFMALEHYNMTTEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSEQRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFIKRTTWDFCCVLLQRPPQKPTALASQGQLPGCIATSSPPPQPESEKAPPARKETRFEEGQRPGQGAPGDAEPVCVPIAMAESDTDDPEEDEENSLSTEEESSKQLPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCTVDTTQAHGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLISLVANALGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSDCESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVGIFTAECIFKMVALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIPKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATIIQRAFRRHLLQRSVKHASFLYRQQAGSSGLSEEDAPEQEGLIAYMMNENFSRRPGPPSSSSVSSTSFPPSYDSVTRATSDNPQVRASDYSPSEDLADFPPTPDRDRESIV

Target ORF information:

RefSeq Version	XM_024982395.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982395.1

ORF Insert Sequence:

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CATACACCAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC TTTACGTACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCAGTCATTT CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGCTTCCCG AAGACAGTTA CTCTGAGGGG AGCACGGCAG ATATGACCAA CACTGCTGAC 
CTCCTGGAGC AGATCCCTGA CCTCGGAGAG GATGTCAAAG ATCCAGAGGA CTGCTTCACT 
GAAGGCTGTG TCCGCCGCTG TCCCTGCTGC ACCGTGGACA CCACACAGGC CCACGGGAAG 
GTCTGGTGGA GGCTGCGCAA GACCTGCTAC CGCATCGTGG AGCACAGCTG GTTCGAGACG 
TTCATCATCT TCATGATCCT GCTCAGCAGT GGCGCACTGG CCTTTGAGGA CATCTACCTG 
GAGGAACGGA AGACCATCAA GGTCCTGCTG GAGTACGCCG ACAAGATGTT CACCTACGTC 
TTCGTGCTGG AGATGCTCCT CAAGTGGGTG GCCTACGGCT TCAAGAAGTA CTTCACCAAC 
GCCTGGTGCT GGCTTGATTT CCTCATCGTG GACGTCTCGC TGATCAGCCT GGTGGCCAAC 
GCCCTGGGCT TTGCTGAGAT GGGCCCCATC AAGTCACTGC GGACCTTGCG TGCGCTCAGA 
CCCCTGCGAG CCCTGTCACG ATTTGAGGGC ATGAGGGTTG TGGTTAACGC CCTGGTGGGC 
GCCATCCCAT CCATCATGAA CGTCCTCCTC GTCTGCCTCA TCTTCTGGCT CATCTTCAGC 
ATCATGGGCG TGAACCTCTT CGCGGGGAAG TTTGGGAGAT GCATCAACCA GACCGAGGGA 
GACCTGCCCT TGAACTATAC CATCGTGAAC AACAAGAGCG ACTGTGAGTC TTTCAACGTG 
ACTGGCGAAT TGTACTGGAC CAAGGTGAAG GTCAACTTTG ACAACGTGGG GGCCGGGTAC 
CTGGCCCTTC TGCAGGTGGC AACATTTAAA GGCTGGATGG ACATCATGTA TGCAGCTGTA 
GACTCCAGGG GGTACGAGGA GCAGCCCCAG TGGGAATACA ACCTCTACAT GTATATCTAT 
TTTGTCATCT TCATCATCTT TGGGTCTTTC TTCACCCTGA ACCTGTTCAT CGGTGTCATC 
ATTGACAACT TCAACCAGCA GAAGAAAAAG TTAGGGGGCC AGGACATCTT CATGACAGAG 
GAGCAGAAGA AGTACTACAA CGCCATGAAG AAGCTGGGCT CCAAGAAGCC CCAGAAGCCC 
ATCCCACGGC CCCTGAACAA GTACCAGGGC TTCATATTCG ACATTGTGAC CAAGCAGGCC 
TTCGACGTCA CCATCATGTT TCTCATCTGC TTAAACATGG TGACCATGAT GGTGGAGACA 
GACGACCAGA GCCCCGAGAA GGTCAACATC TTGGCCAAGA TCAACCTGCT GTTCGTGGGC 
ATCTTCACAG CCGAGTGTAT CTTCAAGATG GTTGCCCTGC GCCACTATTA CTTCACCAAC 
AGCTGGAACA TCTTCGACTT CGTGGTTGTC ATCCTCTCCA TCGTAGGCAC TGTGCTCTCA 
GACATCATCC AGAAGTACTT CTTCTCCCCG ACGCTCTTCC GCGTCATCCG CCTGGCCCGC 
ATAGGCCGCA TCCTCAGGCT GATCCGCGGG GCCAAGGGCA TCCGCACGCT GCTCTTCGCC 
CTCATGATGT CCCTGCCCGC GCTCTTCAAC ATCGGGCTGC TGCTCTTCCT CGTCATGTTC 
ATCTACTCCA TCTTCGGCAT GGCCAACTTC GCCTACGTCA AGTGGGAGGC TGGCATCGAC 
GACATGTTCA ACTTCCAGAC CTTCGCCAAC AGCATGCTGT GCCTCTTCCA GATCACCACG 
TCGGCGGGCT GGGATGGGCT CCTCAGCCCC ATCCTCAACA CGGGGCCCCC CTACTGTGAC 
CCCAACCTGC CCAACAGCAA CGGCTCCCGG GGCAACTGCG GGAGCCCTGC GGTGGGCATC 
CTCTTCTTCA CCACCTACAT CATCATCTCC TTCCTCATTG TGGTCAACAT GTACATCGCC 
ATCATCCTGG AGAACTTCAG CGTGGCCACG GAGGAGAGCA CGGAGCCCCT GAGTGAGGAT 
GACTTCGACA TGTTCTACGA GATCTGGGAG AAGTTCGACC CGGAGGCCAC CCAGTTCATC 
GAGTATTTGG CCCTGTCTGA CTTCGCCGAT GCCCTGTCAG AGCCACTCCG GATCCCCAAG 
CCCAACCAGA TAAGCCTCAT CAATATGGAC CTGCCCATGG TGAGTGGAGA CCGCATCCAC 
TGCATGGACA TCCTCTTTGC CTTCACCAAG AGGGTCCTGG GCGAATCTGG GGAGATGGAC 
GCCCTGAAGA TCCAGATGGA GGAGAAGTTC ATGGCGGCCA ACCCGTCCAA GATCTCCTAC 
GAGCCCATCA CCACCACGCT GCGGCGGAAG CACGAGGAGG TGTCGGCCAC GATCATCCAG 
CGGGCCTTCC GCCGGCACCT GCTGCAGCGC TCCGTCAAGC ACGCCTCCTT CCTCTACCGC 
CAGCAGGCGG GCAGCAGCGG CCTCTCGGAG GAGGACGCCC CCGAGCAGGA GGGCCTCATC 
GCCTACATGA TGAACGAGAA CTTCTCCCGC CGCCCCGGCC CGCCCTCCAG CTCCTCCGTC 
TCCTCCACGT CCTTCCCGCC CTCCTATGAC AGCGTCACCA GGGCCACCAG CGACAACCCC 
CAGGTGCGGG CGTCTGACTA CAGCCCAAGC GAGGATCTCG CAGACTTCCC CCCAACCCCC 
GACAGGGACC GTGAGTCAAT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN5A ( XM_024982396.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838164.1 Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X5, mRNA.

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CloneID	OBa228733
Clone ID Related Accession (Same CDS sequence)	XM_024982396.1
Accession Version	XM_024982396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 5 subunit alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CGTATGTATC AGAAAATATA AAACTAGGCA ATTTGTCGGC TCTTCGAACT 
TTCAGAGTCC TGAGAGCTCT AAAAACTATT TCAGTTATCC CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGCTTCCCG AAGACAGTTA CTCTGAGGGG AGCACGGCAG ATATGACCAA CACTGCTGAC 
CTCCTGGAGC AGATCCCTGA CCTCGGAGAG GATGTCAAAG ATCCAGAGGA CTGCTTCACT 
GAAGGCTGTG TCCGCCGCTG TCCCTGCTGC ACCGTGGACA CCACACAGGC CCACGGGAAG 
GTCTGGTGGA GGCTGCGCAA GACCTGCTAC CGCATCGTGG AGCACAGCTG GTTCGAGACG 
TTCATCATCT TCATGATCCT GCTCAGCAGT GGCGCACTGG CCTTTGAGGA CATCTACCTG 
GAGGAACGGA AGACCATCAA GGTCCTGCTG GAGTACGCCG ACAAGATGTT CACCTACGTC 
TTCGTGCTGG AGATGCTCCT CAAGTGGGTG GCCTACGGCT TCAAGAAGTA CTTCACCAAC 
GCCTGGTGCT GGCTTGATTT CCTCATCGTG GACGTCTCGC TGATCAGCCT GGTGGCCAAC 
GCCCTGGGCT TTGCTGAGAT GGGCCCCATC AAGTCACTGC GGACCTTGCG TGCGCTCAGA 
CCCCTGCGAG CCCTGTCACG ATTTGAGGGC ATGAGGGTTG TGGTTAACGC CCTGGTGGGC 
GCCATCCCAT CCATCATGAA CGTCCTCCTC GTCTGCCTCA TCTTCTGGCT CATCTTCAGC 
ATCATGGGCG TGAACCTCTT CGCGGGGAAG TTTGGGAGAT GCATCAACCA GACCGAGGGA 
GACCTGCCCT TGAACTATAC CATCGTGAAC AACAAGAGCG ACTGTGAGTC TTTCAACGTG 
ACTGGCGAAT TGTACTGGAC CAAGGTGAAG GTCAACTTTG ACAACGTGGG GGCCGGGTAC 
CTGGCCCTTC TGCAGGTGGC AACATTTAAA GGCTGGATGG ACATCATGTA TGCAGCTGTA 
GACTCCAGGG GGTACGAGGA GCAGCCCCAG TGGGAATACA ACCTCTACAT GTATATCTAT 
TTTGTCATCT TCATCATCTT TGGGTCTTTC TTCACCCTGA ACCTGTTCAT CGGTGTCATC 
ATTGACAACT TCAACCAGCA GAAGAAAAAG TTAGGGGGCC AGGACATCTT CATGACAGAG 
GAGCAGAAGA AGTACTACAA CGCCATGAAG AAGCTGGGCT CCAAGAAGCC CCAGAAGCCC 
ATCCCACGGC CCCTGAACAA GTACCAGGGC TTCATATTCG ACATTGTGAC CAAGCAGGCC 
TTCGACGTCA CCATCATGTT TCTCATCTGC TTAAACATGG TGACCATGAT GGTGGAGACA 
GACGACCAGA GCCCCGAGAA GGTCAACATC TTGGCCAAGA TCAACCTGCT GTTCGTGGGC 
ATCTTCACAG CCGAGTGTAT CTTCAAGATG GTTGCCCTGC GCCACTATTA CTTCACCAAC 
AGCTGGAACA TCTTCGACTT CGTGGTTGTC ATCCTCTCCA TCGTAGGCAC TGTGCTCTCA 
GACATCATCC AGAAGTACTT CTTCTCCCCG ACGCTCTTCC GCGTCATCCG CCTGGCCCGC 
ATAGGCCGCA TCCTCAGGCT GATCCGCGGG GCCAAGGGCA TCCGCACGCT GCTCTTCGCC 
CTCATGATGT CCCTGCCCGC GCTCTTCAAC ATCGGGCTGC TGCTCTTCCT CGTCATGTTC 
ATCTACTCCA TCTTCGGCAT GGCCAACTTC GCCTACGTCA AGTGGGAGGC TGGCATCGAC 
GACATGTTCA ACTTCCAGAC CTTCGCCAAC AGCATGCTGT GCCTCTTCCA GATCACCACG 
TCGGCGGGCT GGGATGGGCT CCTCAGCCCC ATCCTCAACA CGGGGCCCCC CTACTGTGAC 
CCCAACCTGC CCAACAGCAA CGGCTCCCGG GGCAACTGCG GGAGCCCTGC GGTGGGCATC 
CTCTTCTTCA CCACCTACAT CATCATCTCC TTCCTCATTG TGGTCAACAT GTACATCGCC 
ATCATCCTGG AGAACTTCAG CGTGGCCACG GAGGAGAGCA CGGAGCCCCT GAGTGAGGAT 
GACTTCGACA TGTTCTACGA GATCTGGGAG AAGTTCGACC CGGAGGCCAC CCAGTTCATC 
GAGTATTTGG CCCTGTCTGA CTTCGCCGAT GCCCTGTCAG AGCCACTCCG GATCCCCAAG 
CCCAACCAGA TAAGCCTCAT CAATATGGAC CTGCCCATGG TGAGTGGAGA CCGCATCCAC 
TGCATGGACA TCCTCTTTGC CTTCACCAAG AGGGTCCTGG GCGAATCTGG GGAGATGGAC 
GCCCTGAAGA TCCAGATGGA GGAGAAGTTC ATGGCGGCCA ACCCGTCCAA GATCTCCTAC 
GAGCCCATCA CCACCACGCT GCGGCGGAAG CACGAGGAGG TGTCGGCCAC GATCATCCAG 
CGGGCCTTCC GCCGGCACCT GCTGCAGCGC TCCGTCAAGC ACGCCTCCTT CCTCTACCGC 
CAGCAGGCGG GCAGCAGCGG CCTCTCGGAG GAGGACGCCC CCGAGCAGGA GGGCCTCATC 
GCCTACATGA TGAACGAGAA CTTCTCCCGC CGCCCCGGCC CGCCCTCCAG CTCCTCCGTC 
TCCTCCACGT CCTTCCCGCC CTCCTATGAC AGCGTCACCA GGGCCACCAG CGACAACCCC 
CAGGTGCGGG CGTCTGACTA CAGCCCAAGC GAGGATCTCG CAGACTTCCC CCCAACCCCC 
GACAGGGACC GTGAGTCAAT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838164.1
CDS	1..5907
Translation	MAAFLLPRGTSSFRRFTRESLAAIEKRMAEKQARSSAASQESRDGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALHVLSPFHPIRRVAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYIFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVSENIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTVLNGTNSTNASVEADGLIWASLDDYLNDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMELLKKEQEALAIRGVDTVSRSSLEMSPLAPVTTHERRSKRRKRMSSGMEECGDDKFPKSDSEDGPRAVNRFSITHGLSRTSMKPRSSHGSIFTFRRRDLGSETDFADDENSTAGDSESHRTSLLVPWPLRRPSTLGQPSPGTSTPGHVLNGKRNSTVDCNGVVSLLGAGDPEATSPGSHLLHPMKLERPPDTTTPSEEPGRPQTLTPQAPCVDGFEEPGERQRALSAVSVLTSALEELEESQRRCPPCWIRFAQHYLIWECCPLWMSIKQKVKFMVMDPFADLTITMCIVLNTLFMALEHYNMTTEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSEQRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFIKRTTWDFCCVLLQRPPQKPTALASQGQLPGCIATSSPPPQPESEKAPPARKETRFEEGQRPGQGAPGDAEPVCVPIAMAESDTDDPEEDEENSLSTEEESSKQLPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCTVDTTQAHGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLISLVANALGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSDCESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVGIFTAECIFKMVALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIPKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATIIQRAFRRHLLQRSVKHASFLYRQQAGSSGLSEEDAPEQEGLIAYMMNENFSRRPGPPSSSSVSSTSFPPSYDSVTRATSDNPQVRASDYSPSEDLADFPPTPDRDRESIV

Target ORF information:

RefSeq Version	XM_024982396.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982396.1

ORF Insert Sequence:

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CGTATGTATC AGAAAATATA AAACTAGGCA ATTTGTCGGC TCTTCGAACT 
TTCAGAGTCC TGAGAGCTCT AAAAACTATT TCAGTTATCC CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGCTTCCCG AAGACAGTTA CTCTGAGGGG AGCACGGCAG ATATGACCAA CACTGCTGAC 
CTCCTGGAGC AGATCCCTGA CCTCGGAGAG GATGTCAAAG ATCCAGAGGA CTGCTTCACT 
GAAGGCTGTG TCCGCCGCTG TCCCTGCTGC ACCGTGGACA CCACACAGGC CCACGGGAAG 
GTCTGGTGGA GGCTGCGCAA GACCTGCTAC CGCATCGTGG AGCACAGCTG GTTCGAGACG 
TTCATCATCT TCATGATCCT GCTCAGCAGT GGCGCACTGG CCTTTGAGGA CATCTACCTG 
GAGGAACGGA AGACCATCAA GGTCCTGCTG GAGTACGCCG ACAAGATGTT CACCTACGTC 
TTCGTGCTGG AGATGCTCCT CAAGTGGGTG GCCTACGGCT TCAAGAAGTA CTTCACCAAC 
GCCTGGTGCT GGCTTGATTT CCTCATCGTG GACGTCTCGC TGATCAGCCT GGTGGCCAAC 
GCCCTGGGCT TTGCTGAGAT GGGCCCCATC AAGTCACTGC GGACCTTGCG TGCGCTCAGA 
CCCCTGCGAG CCCTGTCACG ATTTGAGGGC ATGAGGGTTG TGGTTAACGC CCTGGTGGGC 
GCCATCCCAT CCATCATGAA CGTCCTCCTC GTCTGCCTCA TCTTCTGGCT CATCTTCAGC 
ATCATGGGCG TGAACCTCTT CGCGGGGAAG TTTGGGAGAT GCATCAACCA GACCGAGGGA 
GACCTGCCCT TGAACTATAC CATCGTGAAC AACAAGAGCG ACTGTGAGTC TTTCAACGTG 
ACTGGCGAAT TGTACTGGAC CAAGGTGAAG GTCAACTTTG ACAACGTGGG GGCCGGGTAC 
CTGGCCCTTC TGCAGGTGGC AACATTTAAA GGCTGGATGG ACATCATGTA TGCAGCTGTA 
GACTCCAGGG GGTACGAGGA GCAGCCCCAG TGGGAATACA ACCTCTACAT GTATATCTAT 
TTTGTCATCT TCATCATCTT TGGGTCTTTC TTCACCCTGA ACCTGTTCAT CGGTGTCATC 
ATTGACAACT TCAACCAGCA GAAGAAAAAG TTAGGGGGCC AGGACATCTT CATGACAGAG 
GAGCAGAAGA AGTACTACAA CGCCATGAAG AAGCTGGGCT CCAAGAAGCC CCAGAAGCCC 
ATCCCACGGC CCCTGAACAA GTACCAGGGC TTCATATTCG ACATTGTGAC CAAGCAGGCC 
TTCGACGTCA CCATCATGTT TCTCATCTGC TTAAACATGG TGACCATGAT GGTGGAGACA 
GACGACCAGA GCCCCGAGAA GGTCAACATC TTGGCCAAGA TCAACCTGCT GTTCGTGGGC 
ATCTTCACAG CCGAGTGTAT CTTCAAGATG GTTGCCCTGC GCCACTATTA CTTCACCAAC 
AGCTGGAACA TCTTCGACTT CGTGGTTGTC ATCCTCTCCA TCGTAGGCAC TGTGCTCTCA 
GACATCATCC AGAAGTACTT CTTCTCCCCG ACGCTCTTCC GCGTCATCCG CCTGGCCCGC 
ATAGGCCGCA TCCTCAGGCT GATCCGCGGG GCCAAGGGCA TCCGCACGCT GCTCTTCGCC 
CTCATGATGT CCCTGCCCGC GCTCTTCAAC ATCGGGCTGC TGCTCTTCCT CGTCATGTTC 
ATCTACTCCA TCTTCGGCAT GGCCAACTTC GCCTACGTCA AGTGGGAGGC TGGCATCGAC 
GACATGTTCA ACTTCCAGAC CTTCGCCAAC AGCATGCTGT GCCTCTTCCA GATCACCACG 
TCGGCGGGCT GGGATGGGCT CCTCAGCCCC ATCCTCAACA CGGGGCCCCC CTACTGTGAC 
CCCAACCTGC CCAACAGCAA CGGCTCCCGG GGCAACTGCG GGAGCCCTGC GGTGGGCATC 
CTCTTCTTCA CCACCTACAT CATCATCTCC TTCCTCATTG TGGTCAACAT GTACATCGCC 
ATCATCCTGG AGAACTTCAG CGTGGCCACG GAGGAGAGCA CGGAGCCCCT GAGTGAGGAT 
GACTTCGACA TGTTCTACGA GATCTGGGAG AAGTTCGACC CGGAGGCCAC CCAGTTCATC 
GAGTATTTGG CCCTGTCTGA CTTCGCCGAT GCCCTGTCAG AGCCACTCCG GATCCCCAAG 
CCCAACCAGA TAAGCCTCAT CAATATGGAC CTGCCCATGG TGAGTGGAGA CCGCATCCAC 
TGCATGGACA TCCTCTTTGC CTTCACCAAG AGGGTCCTGG GCGAATCTGG GGAGATGGAC 
GCCCTGAAGA TCCAGATGGA GGAGAAGTTC ATGGCGGCCA ACCCGTCCAA GATCTCCTAC 
GAGCCCATCA CCACCACGCT GCGGCGGAAG CACGAGGAGG TGTCGGCCAC GATCATCCAG 
CGGGCCTTCC GCCGGCACCT GCTGCAGCGC TCCGTCAAGC ACGCCTCCTT CCTCTACCGC 
CAGCAGGCGG GCAGCAGCGG CCTCTCGGAG GAGGACGCCC CCGAGCAGGA GGGCCTCATC 
GCCTACATGA TGAACGAGAA CTTCTCCCGC CGCCCCGGCC CGCCCTCCAG CTCCTCCGTC 
TCCTCCACGT CCTTCCCGCC CTCCTATGAC AGCGTCACCA GGGCCACCAG CGACAACCCC 
CAGGTGCGGG CGTCTGACTA CAGCCCAAGC GAGGATCTCG CAGACTTCCC CCCAACCCCC 
GACAGGGACC GTGAGTCAAT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN5A ( XM_024982397.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838165.1 Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X6, mRNA.

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CloneID	OBa228734
Clone ID Related Accession (Same CDS sequence)	XM_024982397.1
Accession Version	XM_024982397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 5 subunit alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCA CGGTCTTCTG CCTCAGCGTC TTCGCCCTCA TCGGCCTTCA GCTCTTCATG 
GGCAACCTGA GGCACAAGTG CGTCCGCAAC TTCACGGTGC TCAACGGCAC CAACAGCACC 
AATGCCTCCG TGGAGGCCGA CGGCCTGATC TGGGCATCGC TGGACGACTA CCTCAACGAC 
CCAGAAAATT ACCTACTCAA GAATGGCACC TCTGACGTGT TACTGTGTGG GAACAGCTCC 
GACGCTGGGA CATGTCCTGA GGGCTACAGG TGCCTGAAGG CAGGTGAGAA CCCTGACCAT 
GGCTACACCA GCTTCGACTC CTTCGCCTGG GCCTTCCTCG CACTCTTCCG ACTGATGACG 
CAGGACTGCT GGGAGCGCCT CTACCAGCAG ACCCTGAGGT CTGCAGGGAA GATCTACATG 
ATCTTCTTCA TGCTGGTCAT CTTCCTGGGC TCCTTCTACT TGGTGAACTT GATCCTGGCT 
GTGGTCGCCA TGGCCTACGA GGAGCAAAAC CAAGCCACCA TCGCAGAGAC AGAGGAGAAG 
GAAAAGCGAT TCCAGGAAGC CATGGAGTTG CTCAAGAAAG AGCAGGAGGC CCTCGCCATC 
AGGGGTGTGG ACACCGTGTC CCGCAGCTCC TTGGAGATGT CCCCATTGGC CCCAGTAACC 
ACCCACGAGA GAAGGAGCAA GAGAAGAAAA CGAATGTCTT CAGGGATGGA AGAGTGTGGG 
GACGACAAGT TCCCCAAGTC CGACTCAGAG GATGGTCCCC GAGCAGTGAA TCGTTTCAGC 
ATCACCCATG GCCTCAGCAG GACCTCCATG AAGCCGCGCT CCAGCCACGG GAGCATTTTC 
ACCTTCCGCC GACGGGACCT GGGCTCCGAG ACAGATTTTG CGGACGATGA AAACAGCACC 
GCCGGGGACA GTGAGAGCCA CCGCACATCA CTGCTGGTGC CTTGGCCCCT GCGGCGGCCT 
AGTACCCTGG GACAGCCCAG TCCCGGAACC TCAACTCCCG GCCACGTGCT CAACGGCAAA 
AGGAACAGCA CTGTGGACTG TAACGGGGTG GTCTCCTTGC TGGGGGCAGG AGACCCCGAG 
GCCACCTCCC CAGGGAGTCA CCTCCTCCAC CCTATGAAGC TGGAGCGCCC CCCAGACACG 
ACCACACCAT CGGAGGAGCC GGGCAGGCCC CAGACGCTGA CGCCCCAGGC TCCGTGTGTA 
GACGGCTTCG AGGAGCCAGG AGAGCGGCAG CGAGCCCTCA GTGCAGTGAG CGTCCTCACC 
AGTGCCCTGG AAGAGCTGGA GGAGTCTCAG CGCAGGTGTC CACCGTGCTG GATCCGTTTT 
GCCCAGCACT ACCTGATCTG GGAGTGCTGC CCGCTGTGGA TGTCCATTAA GCAGAAAGTG 
AAGTTCATGG TCATGGACCC ATTTGCTGAC CTCACCATCA CCATGTGCAT CGTGCTTAAC 
ACGCTCTTCA TGGCACTGGA GCACTACAAC ATGACGACCG AATTTGAGGA GATGCTGCAG 
GTTGGAAACC TGGTCTTCAC AGGAATCTTC ACAGCAGAGA TGACCTTCAA GATCATTGCC 
TTGGACCCCT ACTACTACTT CCAGCAGGGC TGGAACATCT TCGACAGCAT CATCGTCATC 
CTCAGCCTCA TGGAGCTGGG CCTGTCCCGC ATGGGCAATC TGTCGGTGCT TCGCTCCTTC 
CGCCTGCTTC GGGTCTTCAA GCTGGCCAAG TCCTGGCCCA CCCTGAACAC ACTCATCAAG 
ATCATTGGGA ACTCAGTGGG CGCGCTAGGC AACCTGACGT TGGTGCTGGC CATCATTGTG 
TTCATCTTCG CTGTGGTGGG CATGCAGCTC TTTGGCAAGA ACTACTCAGA GCAGAGGCAC 
CGTATCAGTG ACTCGGGCCT CCTGCCCCGC TGGCACATGA TGGACTTCTT CCATGCCTTC 
CTCATCATCT TCCGCATCCT CTGTGGAGAG TGGATCGAGA CCATGTGGGA CTGCATGGAG 
GTGTCTGGGC AGTCACTATG CCTGCTGGTC TTCCTGCTTG TTATGGTCAT TGGTAACCTC 
GTGGTCCTGA ACCTCTTCCT GGCTTTACTG CTCAGCTCCT TCAGCGCAGA CAACCTCACA 
GCTCCTGACG AGGATGGGGA GATGAACAAC CTCCAGCTGG CTCTGGCCCG CATCCAGCGA 
GGCCTGCGCT TCATCAAGCG GACCACCTGG GACTTCTGCT GCGTGCTCCT GCAGCGGCCG 
CCTCAGAAGC CCACGGCCCT CGCCTCCCAG GGCCAGCTGC CAGGCTGTAT CGCCACCTCC 
AGCCCCCCAC CCCAACCAGA GAGCGAGAAG GCGCCCCCAG CCCGCAAGGA GACGCGGTTT 
GAGGAAGGCC AGCGGCCAGG TCAGGGCGCA CCTGGGGATG CCGAGCCTGT GTGTGTGCCC 
ATCGCCATGG CCGAGTCAGA CACGGATGAC CCCGAGGAGG ATGAGGAGAA CAGCCTAAGC 
ACAGAGGAAG AGTCCAGCAA GCAGCAGGAA TCCCAGCCGG CGTCCGGCAG CCCAGAGGCC 
CTCCCAGAGC CGAGGGTCTG GAGCCAGGTG TCGGAGACCA CCTCCTCTGG GGCCGAGGCC 
AGTGAGGTTC AGGCAGACTT GCGGCAGCAG CGGCGAGCAG AGGCCCCCGC CCCAGGGTGC 
AGTGAGCTTC CCGAAGACAG TTACTCTGAG GGGAGCACGG CAGATATGAC CAACACTGCT 
GACCTCCTGG AGCAGATCCC TGACCTCGGA GAGGATGTCA AAGATCCAGA GGACTGCTTC 
ACTGAAGGCT GTGTCCGCCG CTGTCCCTGC TGCACCGTGG ACACCACACA GGCCCACGGG 
AAGGTCTGGT GGAGGCTGCG CAAGACCTGC TACCGCATCG TGGAGCACAG CTGGTTCGAG 
ACGTTCATCA TCTTCATGAT CCTGCTCAGC AGTGGCGCAC TGGCCTTTGA GGACATCTAC 
CTGGAGGAAC GGAAGACCAT CAAGGTCCTG CTGGAGTACG CCGACAAGAT GTTCACCTAC 
GTCTTCGTGC TGGAGATGCT CCTCAAGTGG GTGGCCTACG GCTTCAAGAA GTACTTCACC 
AACGCCTGGT GCTGGCTTGA TTTCCTCATC GTGGACGTCT CGCTGATCAG CCTGGTGGCC 
AACGCCCTGG GCTTTGCTGA GATGGGCCCC ATCAAGTCAC TGCGGACCTT GCGTGCGCTC 
AGACCCCTGC GAGCCCTGTC ACGATTTGAG GGCATGAGGG TTGTGGTTAA CGCCCTGGTG 
GGCGCCATCC CATCCATCAT GAACGTCCTC CTCGTCTGCC TCATCTTCTG GCTCATCTTC 
AGCATCATGG GCGTGAACCT CTTCGCGGGG AAGTTTGGGA GATGCATCAA CCAGACCGAG 
GGAGACCTGC CCTTGAACTA TACCATCGTG AACAACAAGA GCGACTGTGA GTCTTTCAAC 
GTGACTGGCG AATTGTACTG GACCAAGGTG AAGGTCAACT TTGACAACGT GGGGGCCGGG 
TACCTGGCCC TTCTGCAGGT GGCAACATTT AAAGGCTGGA TGGACATCAT GTATGCAGCT 
GTAGACTCCA GGGGGTACGA GGAGCAGCCC CAGTGGGAAT ACAACCTCTA CATGTATATC 
TATTTTGTCA TCTTCATCAT CTTTGGGTCT TTCTTCACCC TGAACCTGTT CATCGGTGTC 
ATCATTGACA ACTTCAACCA GCAGAAGAAA AAGTTAGGGG GCCAGGACAT CTTCATGACA 
GAGGAGCAGA AGAAGTACTA CAACGCCATG AAGAAGCTGG GCTCCAAGAA GCCCCAGAAG 
CCCATCCCAC GGCCCCTGAA CAAGTACCAG GGCTTCATAT TCGACATTGT GACCAAGCAG 
GCCTTCGACG TCACCATCAT GTTTCTCATC TGCTTAAACA TGGTGACCAT GATGGTGGAG 
ACAGACGACC AGAGCCCCGA GAAGGTCAAC ATCTTGGCCA AGATCAACCT GCTGTTCGTG 
GGCATCTTCA CAGCCGAGTG TATCTTCAAG ATGGTTGCCC TGCGCCACTA TTACTTCACC 
AACAGCTGGA ACATCTTCGA CTTCGTGGTT GTCATCCTCT CCATCGTAGG CACTGTGCTC 
TCAGACATCA TCCAGAAGTA CTTCTTCTCC CCGACGCTCT TCCGCGTCAT CCGCCTGGCC 
CGCATAGGCC GCATCCTCAG GCTGATCCGC GGGGCCAAGG GCATCCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCGCTCTTC AACATCGGGC TGCTGCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAAC TTCGCCTACG TCAAGTGGGA GGCTGGCATC 
GACGACATGT TCAACTTCCA GACCTTCGCC AACAGCATGC TGTGCCTCTT CCAGATCACC 
ACGTCGGCGG GCTGGGATGG GCTCCTCAGC CCCATCCTCA ACACGGGGCC CCCCTACTGT 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGCAACT GCGGGAGCCC TGCGGTGGGC 
ATCCTCTTCT TCACCACCTA CATCATCATC TCCTTCCTCA TTGTGGTCAA CATGTACATC 
GCCATCATCC TGGAGAACTT CAGCGTGGCC ACGGAGGAGA GCACGGAGCC CCTGAGTGAG 
GATGACTTCG ACATGTTCTA CGAGATCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATCGAGTATT TGGCCCTGTC TGACTTCGCC GATGCCCTGT CAGAGCCACT CCGGATCCCC 
AAGCCCAACC AGATAAGCCT CATCAATATG GACCTGCCCA TGGTGAGTGG AGACCGCATC 
CACTGCATGG ACATCCTCTT TGCCTTCACC AAGAGGGTCC TGGGCGAATC TGGGGAGATG 
GACGCCCTGA AGATCCAGAT GGAGGAGAAG TTCATGGCGG CCAACCCGTC CAAGATCTCC 
TACGAGCCCA TCACCACCAC GCTGCGGCGG AAGCACGAGG AGGTGTCGGC CACGATCATC 
CAGCGGGCCT TCCGCCGGCA CCTGCTGCAG CGCTCCGTCA AGCACGCCTC CTTCCTCTAC 
CGCCAGCAGG CGGGCAGCAG CGGCCTCTCG GAGGAGGACG CCCCCGAGCA GGAGGGCCTC 
ATCGCCTACA TGATGAACGA GAACTTCTCC CGCCGCCCCG GCCCGCCCTC CAGCTCCTCC 
GTCTCCTCCA CGTCCTTCCC GCCCTCCTAT GACAGCGTCA CCAGGGCCAC CAGCGACAAC 
CCCCAGGTGC GGGCGTCTGA CTACAGCCCA AGCGAGGATC TCGCAGACTT CCCCCCAACC 
CCCGACAGGG ACCGTGAGTC AATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838165.1
CDS	144..5453
Translation	MVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTVLNGTNSTNASVEADGLIWASLDDYLNDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMELLKKEQEALAIRGVDTVSRSSLEMSPLAPVTTHERRSKRRKRMSSGMEECGDDKFPKSDSEDGPRAVNRFSITHGLSRTSMKPRSSHGSIFTFRRRDLGSETDFADDENSTAGDSESHRTSLLVPWPLRRPSTLGQPSPGTSTPGHVLNGKRNSTVDCNGVVSLLGAGDPEATSPGSHLLHPMKLERPPDTTTPSEEPGRPQTLTPQAPCVDGFEEPGERQRALSAVSVLTSALEELEESQRRCPPCWIRFAQHYLIWECCPLWMSIKQKVKFMVMDPFADLTITMCIVLNTLFMALEHYNMTTEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSEQRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFIKRTTWDFCCVLLQRPPQKPTALASQGQLPGCIATSSPPPQPESEKAPPARKETRFEEGQRPGQGAPGDAEPVCVPIAMAESDTDDPEEDEENSLSTEEESSKQQESQPASGSPEALPEPRVWSQVSETTSSGAEASEVQADLRQQRRAEAPAPGCSELPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCTVDTTQAHGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLISLVANALGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSDCESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVGIFTAECIFKMVALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIPKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATIIQRAFRRHLLQRSVKHASFLYRQQAGSSGLSEEDAPEQEGLIAYMMNENFSRRPGPPSSSSVSSTSFPPSYDSVTRATSDNPQVRASDYSPSEDLADFPPTPDRDRESIV

Target ORF information:

RefSeq Version	XM_024982397.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982397.1

ORF Insert Sequence:

ATGGTCCTCA CGGTCTTCTG CCTCAGCGTC TTCGCCCTCA TCGGCCTTCA GCTCTTCATG 
GGCAACCTGA GGCACAAGTG CGTCCGCAAC TTCACGGTGC TCAACGGCAC CAACAGCACC 
AATGCCTCCG TGGAGGCCGA CGGCCTGATC TGGGCATCGC TGGACGACTA CCTCAACGAC 
CCAGAAAATT ACCTACTCAA GAATGGCACC TCTGACGTGT TACTGTGTGG GAACAGCTCC 
GACGCTGGGA CATGTCCTGA GGGCTACAGG TGCCTGAAGG CAGGTGAGAA CCCTGACCAT 
GGCTACACCA GCTTCGACTC CTTCGCCTGG GCCTTCCTCG CACTCTTCCG ACTGATGACG 
CAGGACTGCT GGGAGCGCCT CTACCAGCAG ACCCTGAGGT CTGCAGGGAA GATCTACATG 
ATCTTCTTCA TGCTGGTCAT CTTCCTGGGC TCCTTCTACT TGGTGAACTT GATCCTGGCT 
GTGGTCGCCA TGGCCTACGA GGAGCAAAAC CAAGCCACCA TCGCAGAGAC AGAGGAGAAG 
GAAAAGCGAT TCCAGGAAGC CATGGAGTTG CTCAAGAAAG AGCAGGAGGC CCTCGCCATC 
AGGGGTGTGG ACACCGTGTC CCGCAGCTCC TTGGAGATGT CCCCATTGGC CCCAGTAACC 
ACCCACGAGA GAAGGAGCAA GAGAAGAAAA CGAATGTCTT CAGGGATGGA AGAGTGTGGG 
GACGACAAGT TCCCCAAGTC CGACTCAGAG GATGGTCCCC GAGCAGTGAA TCGTTTCAGC 
ATCACCCATG GCCTCAGCAG GACCTCCATG AAGCCGCGCT CCAGCCACGG GAGCATTTTC 
ACCTTCCGCC GACGGGACCT GGGCTCCGAG ACAGATTTTG CGGACGATGA AAACAGCACC 
GCCGGGGACA GTGAGAGCCA CCGCACATCA CTGCTGGTGC CTTGGCCCCT GCGGCGGCCT 
AGTACCCTGG GACAGCCCAG TCCCGGAACC TCAACTCCCG GCCACGTGCT CAACGGCAAA 
AGGAACAGCA CTGTGGACTG TAACGGGGTG GTCTCCTTGC TGGGGGCAGG AGACCCCGAG 
GCCACCTCCC CAGGGAGTCA CCTCCTCCAC CCTATGAAGC TGGAGCGCCC CCCAGACACG 
ACCACACCAT CGGAGGAGCC GGGCAGGCCC CAGACGCTGA CGCCCCAGGC TCCGTGTGTA 
GACGGCTTCG AGGAGCCAGG AGAGCGGCAG CGAGCCCTCA GTGCAGTGAG CGTCCTCACC 
AGTGCCCTGG AAGAGCTGGA GGAGTCTCAG CGCAGGTGTC CACCGTGCTG GATCCGTTTT 
GCCCAGCACT ACCTGATCTG GGAGTGCTGC CCGCTGTGGA TGTCCATTAA GCAGAAAGTG 
AAGTTCATGG TCATGGACCC ATTTGCTGAC CTCACCATCA CCATGTGCAT CGTGCTTAAC 
ACGCTCTTCA TGGCACTGGA GCACTACAAC ATGACGACCG AATTTGAGGA GATGCTGCAG 
GTTGGAAACC TGGTCTTCAC AGGAATCTTC ACAGCAGAGA TGACCTTCAA GATCATTGCC 
TTGGACCCCT ACTACTACTT CCAGCAGGGC TGGAACATCT TCGACAGCAT CATCGTCATC 
CTCAGCCTCA TGGAGCTGGG CCTGTCCCGC ATGGGCAATC TGTCGGTGCT TCGCTCCTTC 
CGCCTGCTTC GGGTCTTCAA GCTGGCCAAG TCCTGGCCCA CCCTGAACAC ACTCATCAAG 
ATCATTGGGA ACTCAGTGGG CGCGCTAGGC AACCTGACGT TGGTGCTGGC CATCATTGTG 
TTCATCTTCG CTGTGGTGGG CATGCAGCTC TTTGGCAAGA ACTACTCAGA GCAGAGGCAC 
CGTATCAGTG ACTCGGGCCT CCTGCCCCGC TGGCACATGA TGGACTTCTT CCATGCCTTC 
CTCATCATCT TCCGCATCCT CTGTGGAGAG TGGATCGAGA CCATGTGGGA CTGCATGGAG 
GTGTCTGGGC AGTCACTATG CCTGCTGGTC TTCCTGCTTG TTATGGTCAT TGGTAACCTC 
GTGGTCCTGA ACCTCTTCCT GGCTTTACTG CTCAGCTCCT TCAGCGCAGA CAACCTCACA 
GCTCCTGACG AGGATGGGGA GATGAACAAC CTCCAGCTGG CTCTGGCCCG CATCCAGCGA 
GGCCTGCGCT TCATCAAGCG GACCACCTGG GACTTCTGCT GCGTGCTCCT GCAGCGGCCG 
CCTCAGAAGC CCACGGCCCT CGCCTCCCAG GGCCAGCTGC CAGGCTGTAT CGCCACCTCC 
AGCCCCCCAC CCCAACCAGA GAGCGAGAAG GCGCCCCCAG CCCGCAAGGA GACGCGGTTT 
GAGGAAGGCC AGCGGCCAGG TCAGGGCGCA CCTGGGGATG CCGAGCCTGT GTGTGTGCCC 
ATCGCCATGG CCGAGTCAGA CACGGATGAC CCCGAGGAGG ATGAGGAGAA CAGCCTAAGC 
ACAGAGGAAG AGTCCAGCAA GCAGCAGGAA TCCCAGCCGG CGTCCGGCAG CCCAGAGGCC 
CTCCCAGAGC CGAGGGTCTG GAGCCAGGTG TCGGAGACCA CCTCCTCTGG GGCCGAGGCC 
AGTGAGGTTC AGGCAGACTT GCGGCAGCAG CGGCGAGCAG AGGCCCCCGC CCCAGGGTGC 
AGTGAGCTTC CCGAAGACAG TTACTCTGAG GGGAGCACGG CAGATATGAC CAACACTGCT 
GACCTCCTGG AGCAGATCCC TGACCTCGGA GAGGATGTCA AAGATCCAGA GGACTGCTTC 
ACTGAAGGCT GTGTCCGCCG CTGTCCCTGC TGCACCGTGG ACACCACACA GGCCCACGGG 
AAGGTCTGGT GGAGGCTGCG CAAGACCTGC TACCGCATCG TGGAGCACAG CTGGTTCGAG 
ACGTTCATCA TCTTCATGAT CCTGCTCAGC AGTGGCGCAC TGGCCTTTGA GGACATCTAC 
CTGGAGGAAC GGAAGACCAT CAAGGTCCTG CTGGAGTACG CCGACAAGAT GTTCACCTAC 
GTCTTCGTGC TGGAGATGCT CCTCAAGTGG GTGGCCTACG GCTTCAAGAA GTACTTCACC 
AACGCCTGGT GCTGGCTTGA TTTCCTCATC GTGGACGTCT CGCTGATCAG CCTGGTGGCC 
AACGCCCTGG GCTTTGCTGA GATGGGCCCC ATCAAGTCAC TGCGGACCTT GCGTGCGCTC 
AGACCCCTGC GAGCCCTGTC ACGATTTGAG GGCATGAGGG TTGTGGTTAA CGCCCTGGTG 
GGCGCCATCC CATCCATCAT GAACGTCCTC CTCGTCTGCC TCATCTTCTG GCTCATCTTC 
AGCATCATGG GCGTGAACCT CTTCGCGGGG AAGTTTGGGA GATGCATCAA CCAGACCGAG 
GGAGACCTGC CCTTGAACTA TACCATCGTG AACAACAAGA GCGACTGTGA GTCTTTCAAC 
GTGACTGGCG AATTGTACTG GACCAAGGTG AAGGTCAACT TTGACAACGT GGGGGCCGGG 
TACCTGGCCC TTCTGCAGGT GGCAACATTT AAAGGCTGGA TGGACATCAT GTATGCAGCT 
GTAGACTCCA GGGGGTACGA GGAGCAGCCC CAGTGGGAAT ACAACCTCTA CATGTATATC 
TATTTTGTCA TCTTCATCAT CTTTGGGTCT TTCTTCACCC TGAACCTGTT CATCGGTGTC 
ATCATTGACA ACTTCAACCA GCAGAAGAAA AAGTTAGGGG GCCAGGACAT CTTCATGACA 
GAGGAGCAGA AGAAGTACTA CAACGCCATG AAGAAGCTGG GCTCCAAGAA GCCCCAGAAG 
CCCATCCCAC GGCCCCTGAA CAAGTACCAG GGCTTCATAT TCGACATTGT GACCAAGCAG 
GCCTTCGACG TCACCATCAT GTTTCTCATC TGCTTAAACA TGGTGACCAT GATGGTGGAG 
ACAGACGACC AGAGCCCCGA GAAGGTCAAC ATCTTGGCCA AGATCAACCT GCTGTTCGTG 
GGCATCTTCA CAGCCGAGTG TATCTTCAAG ATGGTTGCCC TGCGCCACTA TTACTTCACC 
AACAGCTGGA ACATCTTCGA CTTCGTGGTT GTCATCCTCT CCATCGTAGG CACTGTGCTC 
TCAGACATCA TCCAGAAGTA CTTCTTCTCC CCGACGCTCT TCCGCGTCAT CCGCCTGGCC 
CGCATAGGCC GCATCCTCAG GCTGATCCGC GGGGCCAAGG GCATCCGCAC GCTGCTCTTC 
GCCCTCATGA TGTCCCTGCC CGCGCTCTTC AACATCGGGC TGCTGCTCTT CCTCGTCATG 
TTCATCTACT CCATCTTCGG CATGGCCAAC TTCGCCTACG TCAAGTGGGA GGCTGGCATC 
GACGACATGT TCAACTTCCA GACCTTCGCC AACAGCATGC TGTGCCTCTT CCAGATCACC 
ACGTCGGCGG GCTGGGATGG GCTCCTCAGC CCCATCCTCA ACACGGGGCC CCCCTACTGT 
GACCCCAACC TGCCCAACAG CAACGGCTCC CGGGGCAACT GCGGGAGCCC TGCGGTGGGC 
ATCCTCTTCT TCACCACCTA CATCATCATC TCCTTCCTCA TTGTGGTCAA CATGTACATC 
GCCATCATCC TGGAGAACTT CAGCGTGGCC ACGGAGGAGA GCACGGAGCC CCTGAGTGAG 
GATGACTTCG ACATGTTCTA CGAGATCTGG GAGAAGTTCG ACCCGGAGGC CACCCAGTTC 
ATCGAGTATT TGGCCCTGTC TGACTTCGCC GATGCCCTGT CAGAGCCACT CCGGATCCCC 
AAGCCCAACC AGATAAGCCT CATCAATATG GACCTGCCCA TGGTGAGTGG AGACCGCATC 
CACTGCATGG ACATCCTCTT TGCCTTCACC AAGAGGGTCC TGGGCGAATC TGGGGAGATG 
GACGCCCTGA AGATCCAGAT GGAGGAGAAG TTCATGGCGG CCAACCCGTC CAAGATCTCC 
TACGAGCCCA TCACCACCAC GCTGCGGCGG AAGCACGAGG AGGTGTCGGC CACGATCATC 
CAGCGGGCCT TCCGCCGGCA CCTGCTGCAG CGCTCCGTCA AGCACGCCTC CTTCCTCTAC 
CGCCAGCAGG CGGGCAGCAG CGGCCTCTCG GAGGAGGACG CCCCCGAGCA GGAGGGCCTC 
ATCGCCTACA TGATGAACGA GAACTTCTCC CGCCGCCCCG GCCCGCCCTC CAGCTCCTCC 
GTCTCCTCCA CGTCCTTCCC GCCCTCCTAT GACAGCGTCA CCAGGGCCAC CAGCGACAAC 
CCCCAGGTGC GGGCGTCTGA CTACAGCCCA AGCGAGGATC TCGCAGACTT CCCCCCAACC 
CCCGACAGGG ACCGTGAGTC AATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN5A ( XM_024982392.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838160.1 Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X1, mRNA.

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CloneID	OBa228729
Clone ID Related Accession (Same CDS sequence)	XM_024982392.1
Accession Version	XM_024982392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 5 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CGTATGTATC AGAAAATATA AAACTAGGCA ATTTGTCGGC TCTTCGAACT 
TTCAGAGTCC TGAGAGCTCT AAAAACTATT TCAGTTATCC CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGCAGGAAT CCCAGCCGGC GTCCGGCAGC CCAGAGGCCC TCCCAGAGCC GAGGGTCTGG 
AGCCAGGTGT CGGAGACCAC CTCCTCTGGG GCCGAGGCCA GTGAGGTTCA GGCAGACTTG 
CGGCAGCAGC GGCGAGCAGA GGCCCCCGCC CCAGGGTGCA GTGAGCTTCC CGAAGACAGT 
TACTCTGAGG GGAGCACGGC AGATATGACC AACACTGCTG ACCTCCTGGA GCAGATCCCT 
GACCTCGGAG AGGATGTCAA AGATCCAGAG GACTGCTTCA CTGAAGGCTG TGTCCGCCGC 
TGTCCCTGCT GCACCGTGGA CACCACACAG GCCCACGGGA AGGTCTGGTG GAGGCTGCGC 
AAGACCTGCT ACCGCATCGT GGAGCACAGC TGGTTCGAGA CGTTCATCAT CTTCATGATC 
CTGCTCAGCA GTGGCGCACT GGCCTTTGAG GACATCTACC TGGAGGAACG GAAGACCATC 
AAGGTCCTGC TGGAGTACGC CGACAAGATG TTCACCTACG TCTTCGTGCT GGAGATGCTC 
CTCAAGTGGG TGGCCTACGG CTTCAAGAAG TACTTCACCA ACGCCTGGTG CTGGCTTGAT 
TTCCTCATCG TGGACGTCTC GCTGATCAGC CTGGTGGCCA ACGCCCTGGG CTTTGCTGAG 
ATGGGCCCCA TCAAGTCACT GCGGACCTTG CGTGCGCTCA GACCCCTGCG AGCCCTGTCA 
CGATTTGAGG GCATGAGGGT TGTGGTTAAC GCCCTGGTGG GCGCCATCCC ATCCATCATG 
AACGTCCTCC TCGTCTGCCT CATCTTCTGG CTCATCTTCA GCATCATGGG CGTGAACCTC 
TTCGCGGGGA AGTTTGGGAG ATGCATCAAC CAGACCGAGG GAGACCTGCC CTTGAACTAT 
ACCATCGTGA ACAACAAGAG CGACTGTGAG TCTTTCAACG TGACTGGCGA ATTGTACTGG 
ACCAAGGTGA AGGTCAACTT TGACAACGTG GGGGCCGGGT ACCTGGCCCT TCTGCAGGTG 
GCAACATTTA AAGGCTGGAT GGACATCATG TATGCAGCTG TAGACTCCAG GGGGTACGAG 
GAGCAGCCCC AGTGGGAATA CAACCTCTAC ATGTATATCT ATTTTGTCAT CTTCATCATC 
TTTGGGTCTT TCTTCACCCT GAACCTGTTC ATCGGTGTCA TCATTGACAA CTTCAACCAG 
CAGAAGAAAA AGTTAGGGGG CCAGGACATC TTCATGACAG AGGAGCAGAA GAAGTACTAC 
AACGCCATGA AGAAGCTGGG CTCCAAGAAG CCCCAGAAGC CCATCCCACG GCCCCTGAAC 
AAGTACCAGG GCTTCATATT CGACATTGTG ACCAAGCAGG CCTTCGACGT CACCATCATG 
TTTCTCATCT GCTTAAACAT GGTGACCATG ATGGTGGAGA CAGACGACCA GAGCCCCGAG 
AAGGTCAACA TCTTGGCCAA GATCAACCTG CTGTTCGTGG GCATCTTCAC AGCCGAGTGT 
ATCTTCAAGA TGGTTGCCCT GCGCCACTAT TACTTCACCA ACAGCTGGAA CATCTTCGAC 
TTCGTGGTTG TCATCCTCTC CATCGTAGGC ACTGTGCTCT CAGACATCAT CCAGAAGTAC 
TTCTTCTCCC CGACGCTCTT CCGCGTCATC CGCCTGGCCC GCATAGGCCG CATCCTCAGG 
CTGATCCGCG GGGCCAAGGG CATCCGCACG CTGCTCTTCG CCCTCATGAT GTCCCTGCCC 
GCGCTCTTCA ACATCGGGCT GCTGCTCTTC CTCGTCATGT TCATCTACTC CATCTTCGGC 
ATGGCCAACT TCGCCTACGT CAAGTGGGAG GCTGGCATCG ACGACATGTT CAACTTCCAG 
ACCTTCGCCA ACAGCATGCT GTGCCTCTTC CAGATCACCA CGTCGGCGGG CTGGGATGGG 
CTCCTCAGCC CCATCCTCAA CACGGGGCCC CCCTACTGTG ACCCCAACCT GCCCAACAGC 
AACGGCTCCC GGGGCAACTG CGGGAGCCCT GCGGTGGGCA TCCTCTTCTT CACCACCTAC 
ATCATCATCT CCTTCCTCAT TGTGGTCAAC ATGTACATCG CCATCATCCT GGAGAACTTC 
AGCGTGGCCA CGGAGGAGAG CACGGAGCCC CTGAGTGAGG ATGACTTCGA CATGTTCTAC 
GAGATCTGGG AGAAGTTCGA CCCGGAGGCC ACCCAGTTCA TCGAGTATTT GGCCCTGTCT 
GACTTCGCCG ATGCCCTGTC AGAGCCACTC CGGATCCCCA AGCCCAACCA GATAAGCCTC 
ATCAATATGG ACCTGCCCAT GGTGAGTGGA GACCGCATCC ACTGCATGGA CATCCTCTTT 
GCCTTCACCA AGAGGGTCCT GGGCGAATCT GGGGAGATGG ACGCCCTGAA GATCCAGATG 
GAGGAGAAGT TCATGGCGGC CAACCCGTCC AAGATCTCCT ACGAGCCCAT CACCACCACG 
CTGCGGCGGA AGCACGAGGA GGTGTCGGCC ACGATCATCC AGCGGGCCTT CCGCCGGCAC 
CTGCTGCAGC GCTCCGTCAA GCACGCCTCC TTCCTCTACC GCCAGCAGGC GGGCAGCAGC 
GGCCTCTCGG AGGAGGACGC CCCCGAGCAG GAGGGCCTCA TCGCCTACAT GATGAACGAG 
AACTTCTCCC GCCGCCCCGG CCCGCCCTCC AGCTCCTCCG TCTCCTCCAC GTCCTTCCCG 
CCCTCCTATG ACAGCGTCAC CAGGGCCACC AGCGACAACC CCCAGGTGCG GGCGTCTGAC 
TACAGCCCAA GCGAGGATCT CGCAGACTTC CCCCCAACCC CCGACAGGGA CCGTGAGTCA 
ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838160.1
CDS	1..6069
Translation	MAAFLLPRGTSSFRRFTRESLAAIEKRMAEKQARSSAASQESRDGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALHVLSPFHPIRRVAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYIFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVSENIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTVLNGTNSTNASVEADGLIWASLDDYLNDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMELLKKEQEALAIRGVDTVSRSSLEMSPLAPVTTHERRSKRRKRMSSGMEECGDDKFPKSDSEDGPRAVNRFSITHGLSRTSMKPRSSHGSIFTFRRRDLGSETDFADDENSTAGDSESHRTSLLVPWPLRRPSTLGQPSPGTSTPGHVLNGKRNSTVDCNGVVSLLGAGDPEATSPGSHLLHPMKLERPPDTTTPSEEPGRPQTLTPQAPCVDGFEEPGERQRALSAVSVLTSALEELEESQRRCPPCWIRFAQHYLIWECCPLWMSIKQKVKFMVMDPFADLTITMCIVLNTLFMALEHYNMTTEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSEQRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFIKRTTWDFCCVLLQRPPQKPTALASQGQLPGCIATSSPPPQPESEKAPPARKETRFEEGQRPGQGAPGDAEPVCVPIAMAESDTDDPEEDEENSLSTEEESSKQQESQPASGSPEALPEPRVWSQVSETTSSGAEASEVQADLRQQRRAEAPAPGCSELPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCTVDTTQAHGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLISLVANALGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSDCESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVGIFTAECIFKMVALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIPKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATIIQRAFRRHLLQRSVKHASFLYRQQAGSSGLSEEDAPEQEGLIAYMMNENFSRRPGPPSSSSVSSTSFPPSYDSVTRATSDNPQVRASDYSPSEDLADFPPTPDRDRESIV

Target ORF information:

RefSeq Version	XM_024982392.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982392.1

ORF Insert Sequence:

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CGTATGTATC AGAAAATATA AAACTAGGCA ATTTGTCGGC TCTTCGAACT 
TTCAGAGTCC TGAGAGCTCT AAAAACTATT TCAGTTATCC CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGCAGGAAT CCCAGCCGGC GTCCGGCAGC CCAGAGGCCC TCCCAGAGCC GAGGGTCTGG 
AGCCAGGTGT CGGAGACCAC CTCCTCTGGG GCCGAGGCCA GTGAGGTTCA GGCAGACTTG 
CGGCAGCAGC GGCGAGCAGA GGCCCCCGCC CCAGGGTGCA GTGAGCTTCC CGAAGACAGT 
TACTCTGAGG GGAGCACGGC AGATATGACC AACACTGCTG ACCTCCTGGA GCAGATCCCT 
GACCTCGGAG AGGATGTCAA AGATCCAGAG GACTGCTTCA CTGAAGGCTG TGTCCGCCGC 
TGTCCCTGCT GCACCGTGGA CACCACACAG GCCCACGGGA AGGTCTGGTG GAGGCTGCGC 
AAGACCTGCT ACCGCATCGT GGAGCACAGC TGGTTCGAGA CGTTCATCAT CTTCATGATC 
CTGCTCAGCA GTGGCGCACT GGCCTTTGAG GACATCTACC TGGAGGAACG GAAGACCATC 
AAGGTCCTGC TGGAGTACGC CGACAAGATG TTCACCTACG TCTTCGTGCT GGAGATGCTC 
CTCAAGTGGG TGGCCTACGG CTTCAAGAAG TACTTCACCA ACGCCTGGTG CTGGCTTGAT 
TTCCTCATCG TGGACGTCTC GCTGATCAGC CTGGTGGCCA ACGCCCTGGG CTTTGCTGAG 
ATGGGCCCCA TCAAGTCACT GCGGACCTTG CGTGCGCTCA GACCCCTGCG AGCCCTGTCA 
CGATTTGAGG GCATGAGGGT TGTGGTTAAC GCCCTGGTGG GCGCCATCCC ATCCATCATG 
AACGTCCTCC TCGTCTGCCT CATCTTCTGG CTCATCTTCA GCATCATGGG CGTGAACCTC 
TTCGCGGGGA AGTTTGGGAG ATGCATCAAC CAGACCGAGG GAGACCTGCC CTTGAACTAT 
ACCATCGTGA ACAACAAGAG CGACTGTGAG TCTTTCAACG TGACTGGCGA ATTGTACTGG 
ACCAAGGTGA AGGTCAACTT TGACAACGTG GGGGCCGGGT ACCTGGCCCT TCTGCAGGTG 
GCAACATTTA AAGGCTGGAT GGACATCATG TATGCAGCTG TAGACTCCAG GGGGTACGAG 
GAGCAGCCCC AGTGGGAATA CAACCTCTAC ATGTATATCT ATTTTGTCAT CTTCATCATC 
TTTGGGTCTT TCTTCACCCT GAACCTGTTC ATCGGTGTCA TCATTGACAA CTTCAACCAG 
CAGAAGAAAA AGTTAGGGGG CCAGGACATC TTCATGACAG AGGAGCAGAA GAAGTACTAC 
AACGCCATGA AGAAGCTGGG CTCCAAGAAG CCCCAGAAGC CCATCCCACG GCCCCTGAAC 
AAGTACCAGG GCTTCATATT CGACATTGTG ACCAAGCAGG CCTTCGACGT CACCATCATG 
TTTCTCATCT GCTTAAACAT GGTGACCATG ATGGTGGAGA CAGACGACCA GAGCCCCGAG 
AAGGTCAACA TCTTGGCCAA GATCAACCTG CTGTTCGTGG GCATCTTCAC AGCCGAGTGT 
ATCTTCAAGA TGGTTGCCCT GCGCCACTAT TACTTCACCA ACAGCTGGAA CATCTTCGAC 
TTCGTGGTTG TCATCCTCTC CATCGTAGGC ACTGTGCTCT CAGACATCAT CCAGAAGTAC 
TTCTTCTCCC CGACGCTCTT CCGCGTCATC CGCCTGGCCC GCATAGGCCG CATCCTCAGG 
CTGATCCGCG GGGCCAAGGG CATCCGCACG CTGCTCTTCG CCCTCATGAT GTCCCTGCCC 
GCGCTCTTCA ACATCGGGCT GCTGCTCTTC CTCGTCATGT TCATCTACTC CATCTTCGGC 
ATGGCCAACT TCGCCTACGT CAAGTGGGAG GCTGGCATCG ACGACATGTT CAACTTCCAG 
ACCTTCGCCA ACAGCATGCT GTGCCTCTTC CAGATCACCA CGTCGGCGGG CTGGGATGGG 
CTCCTCAGCC CCATCCTCAA CACGGGGCCC CCCTACTGTG ACCCCAACCT GCCCAACAGC 
AACGGCTCCC GGGGCAACTG CGGGAGCCCT GCGGTGGGCA TCCTCTTCTT CACCACCTAC 
ATCATCATCT CCTTCCTCAT TGTGGTCAAC ATGTACATCG CCATCATCCT GGAGAACTTC 
AGCGTGGCCA CGGAGGAGAG CACGGAGCCC CTGAGTGAGG ATGACTTCGA CATGTTCTAC 
GAGATCTGGG AGAAGTTCGA CCCGGAGGCC ACCCAGTTCA TCGAGTATTT GGCCCTGTCT 
GACTTCGCCG ATGCCCTGTC AGAGCCACTC CGGATCCCCA AGCCCAACCA GATAAGCCTC 
ATCAATATGG ACCTGCCCAT GGTGAGTGGA GACCGCATCC ACTGCATGGA CATCCTCTTT 
GCCTTCACCA AGAGGGTCCT GGGCGAATCT GGGGAGATGG ACGCCCTGAA GATCCAGATG 
GAGGAGAAGT TCATGGCGGC CAACCCGTCC AAGATCTCCT ACGAGCCCAT CACCACCACG 
CTGCGGCGGA AGCACGAGGA GGTGTCGGCC ACGATCATCC AGCGGGCCTT CCGCCGGCAC 
CTGCTGCAGC GCTCCGTCAA GCACGCCTCC TTCCTCTACC GCCAGCAGGC GGGCAGCAGC 
GGCCTCTCGG AGGAGGACGC CCCCGAGCAG GAGGGCCTCA TCGCCTACAT GATGAACGAG 
AACTTCTCCC GCCGCCCCGG CCCGCCCTCC AGCTCCTCCG TCTCCTCCAC GTCCTTCCCG 
CCCTCCTATG ACAGCGTCAC CAGGGCCACC AGCGACAACC CCCAGGTGCG GGCGTCTGAC 
TACAGCCCAA GCGAGGATCT CGCAGACTTC CCCCCAACCC CCGACAGGGA CCGTGAGTCA 
ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN5A ( XM_024982393.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838161.1 Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X2, mRNA.

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CloneID	OBa228730
Clone ID Related Accession (Same CDS sequence)	XM_024982393.1
Accession Version	XM_024982393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 5 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CATACACCAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC TTTACGTACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCAGTCATTT CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGGAATCCC AGCCGGCGTC CGGCAGCCCA GAGGCCCTCC CAGAGCCGAG GGTCTGGAGC 
CAGGTGTCGG AGACCACCTC CTCTGGGGCC GAGGCCAGTG AGGTTCAGGC AGACTTGCGG 
CAGCAGCGGC GAGCAGAGGC CCCCGCCCCA GGGTGCAGTG AGCTTCCCGA AGACAGTTAC 
TCTGAGGGGA GCACGGCAGA TATGACCAAC ACTGCTGACC TCCTGGAGCA GATCCCTGAC 
CTCGGAGAGG ATGTCAAAGA TCCAGAGGAC TGCTTCACTG AAGGCTGTGT CCGCCGCTGT 
CCCTGCTGCA CCGTGGACAC CACACAGGCC CACGGGAAGG TCTGGTGGAG GCTGCGCAAG 
ACCTGCTACC GCATCGTGGA GCACAGCTGG TTCGAGACGT TCATCATCTT CATGATCCTG 
CTCAGCAGTG GCGCACTGGC CTTTGAGGAC ATCTACCTGG AGGAACGGAA GACCATCAAG 
GTCCTGCTGG AGTACGCCGA CAAGATGTTC ACCTACGTCT TCGTGCTGGA GATGCTCCTC 
AAGTGGGTGG CCTACGGCTT CAAGAAGTAC TTCACCAACG CCTGGTGCTG GCTTGATTTC 
CTCATCGTGG ACGTCTCGCT GATCAGCCTG GTGGCCAACG CCCTGGGCTT TGCTGAGATG 
GGCCCCATCA AGTCACTGCG GACCTTGCGT GCGCTCAGAC CCCTGCGAGC CCTGTCACGA 
TTTGAGGGCA TGAGGGTTGT GGTTAACGCC CTGGTGGGCG CCATCCCATC CATCATGAAC 
GTCCTCCTCG TCTGCCTCAT CTTCTGGCTC ATCTTCAGCA TCATGGGCGT GAACCTCTTC 
GCGGGGAAGT TTGGGAGATG CATCAACCAG ACCGAGGGAG ACCTGCCCTT GAACTATACC 
ATCGTGAACA ACAAGAGCGA CTGTGAGTCT TTCAACGTGA CTGGCGAATT GTACTGGACC 
AAGGTGAAGG TCAACTTTGA CAACGTGGGG GCCGGGTACC TGGCCCTTCT GCAGGTGGCA 
ACATTTAAAG GCTGGATGGA CATCATGTAT GCAGCTGTAG ACTCCAGGGG GTACGAGGAG 
CAGCCCCAGT GGGAATACAA CCTCTACATG TATATCTATT TTGTCATCTT CATCATCTTT 
GGGTCTTTCT TCACCCTGAA CCTGTTCATC GGTGTCATCA TTGACAACTT CAACCAGCAG 
AAGAAAAAGT TAGGGGGCCA GGACATCTTC ATGACAGAGG AGCAGAAGAA GTACTACAAC 
GCCATGAAGA AGCTGGGCTC CAAGAAGCCC CAGAAGCCCA TCCCACGGCC CCTGAACAAG 
TACCAGGGCT TCATATTCGA CATTGTGACC AAGCAGGCCT TCGACGTCAC CATCATGTTT 
CTCATCTGCT TAAACATGGT GACCATGATG GTGGAGACAG ACGACCAGAG CCCCGAGAAG 
GTCAACATCT TGGCCAAGAT CAACCTGCTG TTCGTGGGCA TCTTCACAGC CGAGTGTATC 
TTCAAGATGG TTGCCCTGCG CCACTATTAC TTCACCAACA GCTGGAACAT CTTCGACTTC 
GTGGTTGTCA TCCTCTCCAT CGTAGGCACT GTGCTCTCAG ACATCATCCA GAAGTACTTC 
TTCTCCCCGA CGCTCTTCCG CGTCATCCGC CTGGCCCGCA TAGGCCGCAT CCTCAGGCTG 
ATCCGCGGGG CCAAGGGCAT CCGCACGCTG CTCTTCGCCC TCATGATGTC CCTGCCCGCG 
CTCTTCAACA TCGGGCTGCT GCTCTTCCTC GTCATGTTCA TCTACTCCAT CTTCGGCATG 
GCCAACTTCG CCTACGTCAA GTGGGAGGCT GGCATCGACG ACATGTTCAA CTTCCAGACC 
TTCGCCAACA GCATGCTGTG CCTCTTCCAG ATCACCACGT CGGCGGGCTG GGATGGGCTC 
CTCAGCCCCA TCCTCAACAC GGGGCCCCCC TACTGTGACC CCAACCTGCC CAACAGCAAC 
GGCTCCCGGG GCAACTGCGG GAGCCCTGCG GTGGGCATCC TCTTCTTCAC CACCTACATC 
ATCATCTCCT TCCTCATTGT GGTCAACATG TACATCGCCA TCATCCTGGA GAACTTCAGC 
GTGGCCACGG AGGAGAGCAC GGAGCCCCTG AGTGAGGATG ACTTCGACAT GTTCTACGAG 
ATCTGGGAGA AGTTCGACCC GGAGGCCACC CAGTTCATCG AGTATTTGGC CCTGTCTGAC 
TTCGCCGATG CCCTGTCAGA GCCACTCCGG ATCCCCAAGC CCAACCAGAT AAGCCTCATC 
AATATGGACC TGCCCATGGT GAGTGGAGAC CGCATCCACT GCATGGACAT CCTCTTTGCC 
TTCACCAAGA GGGTCCTGGG CGAATCTGGG GAGATGGACG CCCTGAAGAT CCAGATGGAG 
GAGAAGTTCA TGGCGGCCAA CCCGTCCAAG ATCTCCTACG AGCCCATCAC CACCACGCTG 
CGGCGGAAGC ACGAGGAGGT GTCGGCCACG ATCATCCAGC GGGCCTTCCG CCGGCACCTG 
CTGCAGCGCT CCGTCAAGCA CGCCTCCTTC CTCTACCGCC AGCAGGCGGG CAGCAGCGGC 
CTCTCGGAGG AGGACGCCCC CGAGCAGGAG GGCCTCATCG CCTACATGAT GAACGAGAAC 
TTCTCCCGCC GCCCCGGCCC GCCCTCCAGC TCCTCCGTCT CCTCCACGTC CTTCCCGCCC 
TCCTATGACA GCGTCACCAG GGCCACCAGC GACAACCCCC AGGTGCGGGC GTCTGACTAC 
AGCCCAAGCG AGGATCTCGC AGACTTCCCC CCAACCCCCG ACAGGGACCG TGAGTCAATC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838161.1
CDS	1..6066
Translation	MAAFLLPRGTSSFRRFTRESLAAIEKRMAEKQARSSAASQESRDGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALHVLSPFHPIRRVAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYIFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTVLNGTNSTNASVEADGLIWASLDDYLNDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMELLKKEQEALAIRGVDTVSRSSLEMSPLAPVTTHERRSKRRKRMSSGMEECGDDKFPKSDSEDGPRAVNRFSITHGLSRTSMKPRSSHGSIFTFRRRDLGSETDFADDENSTAGDSESHRTSLLVPWPLRRPSTLGQPSPGTSTPGHVLNGKRNSTVDCNGVVSLLGAGDPEATSPGSHLLHPMKLERPPDTTTPSEEPGRPQTLTPQAPCVDGFEEPGERQRALSAVSVLTSALEELEESQRRCPPCWIRFAQHYLIWECCPLWMSIKQKVKFMVMDPFADLTITMCIVLNTLFMALEHYNMTTEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSEQRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFIKRTTWDFCCVLLQRPPQKPTALASQGQLPGCIATSSPPPQPESEKAPPARKETRFEEGQRPGQGAPGDAEPVCVPIAMAESDTDDPEEDEENSLSTEEESSKQESQPASGSPEALPEPRVWSQVSETTSSGAEASEVQADLRQQRRAEAPAPGCSELPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCTVDTTQAHGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLISLVANALGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSDCESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVGIFTAECIFKMVALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIPKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATIIQRAFRRHLLQRSVKHASFLYRQQAGSSGLSEEDAPEQEGLIAYMMNENFSRRPGPPSSSSVSSTSFPPSYDSVTRATSDNPQVRASDYSPSEDLADFPPTPDRDRESIV

Target ORF information:

RefSeq Version	XM_024982393.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982393.1

ORF Insert Sequence:

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CATACACCAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC TTTACGTACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCAGTCATTT CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGGAATCCC AGCCGGCGTC CGGCAGCCCA GAGGCCCTCC CAGAGCCGAG GGTCTGGAGC 
CAGGTGTCGG AGACCACCTC CTCTGGGGCC GAGGCCAGTG AGGTTCAGGC AGACTTGCGG 
CAGCAGCGGC GAGCAGAGGC CCCCGCCCCA GGGTGCAGTG AGCTTCCCGA AGACAGTTAC 
TCTGAGGGGA GCACGGCAGA TATGACCAAC ACTGCTGACC TCCTGGAGCA GATCCCTGAC 
CTCGGAGAGG ATGTCAAAGA TCCAGAGGAC TGCTTCACTG AAGGCTGTGT CCGCCGCTGT 
CCCTGCTGCA CCGTGGACAC CACACAGGCC CACGGGAAGG TCTGGTGGAG GCTGCGCAAG 
ACCTGCTACC GCATCGTGGA GCACAGCTGG TTCGAGACGT TCATCATCTT CATGATCCTG 
CTCAGCAGTG GCGCACTGGC CTTTGAGGAC ATCTACCTGG AGGAACGGAA GACCATCAAG 
GTCCTGCTGG AGTACGCCGA CAAGATGTTC ACCTACGTCT TCGTGCTGGA GATGCTCCTC 
AAGTGGGTGG CCTACGGCTT CAAGAAGTAC TTCACCAACG CCTGGTGCTG GCTTGATTTC 
CTCATCGTGG ACGTCTCGCT GATCAGCCTG GTGGCCAACG CCCTGGGCTT TGCTGAGATG 
GGCCCCATCA AGTCACTGCG GACCTTGCGT GCGCTCAGAC CCCTGCGAGC CCTGTCACGA 
TTTGAGGGCA TGAGGGTTGT GGTTAACGCC CTGGTGGGCG CCATCCCATC CATCATGAAC 
GTCCTCCTCG TCTGCCTCAT CTTCTGGCTC ATCTTCAGCA TCATGGGCGT GAACCTCTTC 
GCGGGGAAGT TTGGGAGATG CATCAACCAG ACCGAGGGAG ACCTGCCCTT GAACTATACC 
ATCGTGAACA ACAAGAGCGA CTGTGAGTCT TTCAACGTGA CTGGCGAATT GTACTGGACC 
AAGGTGAAGG TCAACTTTGA CAACGTGGGG GCCGGGTACC TGGCCCTTCT GCAGGTGGCA 
ACATTTAAAG GCTGGATGGA CATCATGTAT GCAGCTGTAG ACTCCAGGGG GTACGAGGAG 
CAGCCCCAGT GGGAATACAA CCTCTACATG TATATCTATT TTGTCATCTT CATCATCTTT 
GGGTCTTTCT TCACCCTGAA CCTGTTCATC GGTGTCATCA TTGACAACTT CAACCAGCAG 
AAGAAAAAGT TAGGGGGCCA GGACATCTTC ATGACAGAGG AGCAGAAGAA GTACTACAAC 
GCCATGAAGA AGCTGGGCTC CAAGAAGCCC CAGAAGCCCA TCCCACGGCC CCTGAACAAG 
TACCAGGGCT TCATATTCGA CATTGTGACC AAGCAGGCCT TCGACGTCAC CATCATGTTT 
CTCATCTGCT TAAACATGGT GACCATGATG GTGGAGACAG ACGACCAGAG CCCCGAGAAG 
GTCAACATCT TGGCCAAGAT CAACCTGCTG TTCGTGGGCA TCTTCACAGC CGAGTGTATC 
TTCAAGATGG TTGCCCTGCG CCACTATTAC TTCACCAACA GCTGGAACAT CTTCGACTTC 
GTGGTTGTCA TCCTCTCCAT CGTAGGCACT GTGCTCTCAG ACATCATCCA GAAGTACTTC 
TTCTCCCCGA CGCTCTTCCG CGTCATCCGC CTGGCCCGCA TAGGCCGCAT CCTCAGGCTG 
ATCCGCGGGG CCAAGGGCAT CCGCACGCTG CTCTTCGCCC TCATGATGTC CCTGCCCGCG 
CTCTTCAACA TCGGGCTGCT GCTCTTCCTC GTCATGTTCA TCTACTCCAT CTTCGGCATG 
GCCAACTTCG CCTACGTCAA GTGGGAGGCT GGCATCGACG ACATGTTCAA CTTCCAGACC 
TTCGCCAACA GCATGCTGTG CCTCTTCCAG ATCACCACGT CGGCGGGCTG GGATGGGCTC 
CTCAGCCCCA TCCTCAACAC GGGGCCCCCC TACTGTGACC CCAACCTGCC CAACAGCAAC 
GGCTCCCGGG GCAACTGCGG GAGCCCTGCG GTGGGCATCC TCTTCTTCAC CACCTACATC 
ATCATCTCCT TCCTCATTGT GGTCAACATG TACATCGCCA TCATCCTGGA GAACTTCAGC 
GTGGCCACGG AGGAGAGCAC GGAGCCCCTG AGTGAGGATG ACTTCGACAT GTTCTACGAG 
ATCTGGGAGA AGTTCGACCC GGAGGCCACC CAGTTCATCG AGTATTTGGC CCTGTCTGAC 
TTCGCCGATG CCCTGTCAGA GCCACTCCGG ATCCCCAAGC CCAACCAGAT AAGCCTCATC 
AATATGGACC TGCCCATGGT GAGTGGAGAC CGCATCCACT GCATGGACAT CCTCTTTGCC 
TTCACCAAGA GGGTCCTGGG CGAATCTGGG GAGATGGACG CCCTGAAGAT CCAGATGGAG 
GAGAAGTTCA TGGCGGCCAA CCCGTCCAAG ATCTCCTACG AGCCCATCAC CACCACGCTG 
CGGCGGAAGC ACGAGGAGGT GTCGGCCACG ATCATCCAGC GGGCCTTCCG CCGGCACCTG 
CTGCAGCGCT CCGTCAAGCA CGCCTCCTTC CTCTACCGCC AGCAGGCGGG CAGCAGCGGC 
CTCTCGGAGG AGGACGCCCC CGAGCAGGAG GGCCTCATCG CCTACATGAT GAACGAGAAC 
TTCTCCCGCC GCCCCGGCCC GCCCTCCAGC TCCTCCGTCT CCTCCACGTC CTTCCCGCCC 
TCCTATGACA GCGTCACCAG GGCCACCAGC GACAACCCCC AGGTGCGGGC GTCTGACTAC 
AGCCCAAGCG AGGATCTCGC AGACTTCCCC CCAACCCCCG ACAGGGACCG TGAGTCAATC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN5A ( XM_024982394.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838162.1 Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X3, mRNA.

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CloneID	OBa228731
Clone ID Related Accession (Same CDS sequence)	XM_024982394.1
Accession Version	XM_024982394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 5 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CGTATGTATC AGAAAATATA AAACTAGGCA ATTTGTCGGC TCTTCGAACT 
TTCAGAGTCC TGAGAGCTCT AAAAACTATT TCAGTTATCC CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGGAATCCC AGCCGGCGTC CGGCAGCCCA GAGGCCCTCC CAGAGCCGAG GGTCTGGAGC 
CAGGTGTCGG AGACCACCTC CTCTGGGGCC GAGGCCAGTG AGGTTCAGGC AGACTTGCGG 
CAGCAGCGGC GAGCAGAGGC CCCCGCCCCA GGGTGCAGTG AGCTTCCCGA AGACAGTTAC 
TCTGAGGGGA GCACGGCAGA TATGACCAAC ACTGCTGACC TCCTGGAGCA GATCCCTGAC 
CTCGGAGAGG ATGTCAAAGA TCCAGAGGAC TGCTTCACTG AAGGCTGTGT CCGCCGCTGT 
CCCTGCTGCA CCGTGGACAC CACACAGGCC CACGGGAAGG TCTGGTGGAG GCTGCGCAAG 
ACCTGCTACC GCATCGTGGA GCACAGCTGG TTCGAGACGT TCATCATCTT CATGATCCTG 
CTCAGCAGTG GCGCACTGGC CTTTGAGGAC ATCTACCTGG AGGAACGGAA GACCATCAAG 
GTCCTGCTGG AGTACGCCGA CAAGATGTTC ACCTACGTCT TCGTGCTGGA GATGCTCCTC 
AAGTGGGTGG CCTACGGCTT CAAGAAGTAC TTCACCAACG CCTGGTGCTG GCTTGATTTC 
CTCATCGTGG ACGTCTCGCT GATCAGCCTG GTGGCCAACG CCCTGGGCTT TGCTGAGATG 
GGCCCCATCA AGTCACTGCG GACCTTGCGT GCGCTCAGAC CCCTGCGAGC CCTGTCACGA 
TTTGAGGGCA TGAGGGTTGT GGTTAACGCC CTGGTGGGCG CCATCCCATC CATCATGAAC 
GTCCTCCTCG TCTGCCTCAT CTTCTGGCTC ATCTTCAGCA TCATGGGCGT GAACCTCTTC 
GCGGGGAAGT TTGGGAGATG CATCAACCAG ACCGAGGGAG ACCTGCCCTT GAACTATACC 
ATCGTGAACA ACAAGAGCGA CTGTGAGTCT TTCAACGTGA CTGGCGAATT GTACTGGACC 
AAGGTGAAGG TCAACTTTGA CAACGTGGGG GCCGGGTACC TGGCCCTTCT GCAGGTGGCA 
ACATTTAAAG GCTGGATGGA CATCATGTAT GCAGCTGTAG ACTCCAGGGG GTACGAGGAG 
CAGCCCCAGT GGGAATACAA CCTCTACATG TATATCTATT TTGTCATCTT CATCATCTTT 
GGGTCTTTCT TCACCCTGAA CCTGTTCATC GGTGTCATCA TTGACAACTT CAACCAGCAG 
AAGAAAAAGT TAGGGGGCCA GGACATCTTC ATGACAGAGG AGCAGAAGAA GTACTACAAC 
GCCATGAAGA AGCTGGGCTC CAAGAAGCCC CAGAAGCCCA TCCCACGGCC CCTGAACAAG 
TACCAGGGCT TCATATTCGA CATTGTGACC AAGCAGGCCT TCGACGTCAC CATCATGTTT 
CTCATCTGCT TAAACATGGT GACCATGATG GTGGAGACAG ACGACCAGAG CCCCGAGAAG 
GTCAACATCT TGGCCAAGAT CAACCTGCTG TTCGTGGGCA TCTTCACAGC CGAGTGTATC 
TTCAAGATGG TTGCCCTGCG CCACTATTAC TTCACCAACA GCTGGAACAT CTTCGACTTC 
GTGGTTGTCA TCCTCTCCAT CGTAGGCACT GTGCTCTCAG ACATCATCCA GAAGTACTTC 
TTCTCCCCGA CGCTCTTCCG CGTCATCCGC CTGGCCCGCA TAGGCCGCAT CCTCAGGCTG 
ATCCGCGGGG CCAAGGGCAT CCGCACGCTG CTCTTCGCCC TCATGATGTC CCTGCCCGCG 
CTCTTCAACA TCGGGCTGCT GCTCTTCCTC GTCATGTTCA TCTACTCCAT CTTCGGCATG 
GCCAACTTCG CCTACGTCAA GTGGGAGGCT GGCATCGACG ACATGTTCAA CTTCCAGACC 
TTCGCCAACA GCATGCTGTG CCTCTTCCAG ATCACCACGT CGGCGGGCTG GGATGGGCTC 
CTCAGCCCCA TCCTCAACAC GGGGCCCCCC TACTGTGACC CCAACCTGCC CAACAGCAAC 
GGCTCCCGGG GCAACTGCGG GAGCCCTGCG GTGGGCATCC TCTTCTTCAC CACCTACATC 
ATCATCTCCT TCCTCATTGT GGTCAACATG TACATCGCCA TCATCCTGGA GAACTTCAGC 
GTGGCCACGG AGGAGAGCAC GGAGCCCCTG AGTGAGGATG ACTTCGACAT GTTCTACGAG 
ATCTGGGAGA AGTTCGACCC GGAGGCCACC CAGTTCATCG AGTATTTGGC CCTGTCTGAC 
TTCGCCGATG CCCTGTCAGA GCCACTCCGG ATCCCCAAGC CCAACCAGAT AAGCCTCATC 
AATATGGACC TGCCCATGGT GAGTGGAGAC CGCATCCACT GCATGGACAT CCTCTTTGCC 
TTCACCAAGA GGGTCCTGGG CGAATCTGGG GAGATGGACG CCCTGAAGAT CCAGATGGAG 
GAGAAGTTCA TGGCGGCCAA CCCGTCCAAG ATCTCCTACG AGCCCATCAC CACCACGCTG 
CGGCGGAAGC ACGAGGAGGT GTCGGCCACG ATCATCCAGC GGGCCTTCCG CCGGCACCTG 
CTGCAGCGCT CCGTCAAGCA CGCCTCCTTC CTCTACCGCC AGCAGGCGGG CAGCAGCGGC 
CTCTCGGAGG AGGACGCCCC CGAGCAGGAG GGCCTCATCG CCTACATGAT GAACGAGAAC 
TTCTCCCGCC GCCCCGGCCC GCCCTCCAGC TCCTCCGTCT CCTCCACGTC CTTCCCGCCC 
TCCTATGACA GCGTCACCAG GGCCACCAGC GACAACCCCC AGGTGCGGGC GTCTGACTAC 
AGCCCAAGCG AGGATCTCGC AGACTTCCCC CCAACCCCCG ACAGGGACCG TGAGTCAATC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838162.1
CDS	1..6066
Translation	MAAFLLPRGTSSFRRFTRESLAAIEKRMAEKQARSSAASQESRDGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALHVLSPFHPIRRVAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYIFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVSENIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTVLNGTNSTNASVEADGLIWASLDDYLNDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMELLKKEQEALAIRGVDTVSRSSLEMSPLAPVTTHERRSKRRKRMSSGMEECGDDKFPKSDSEDGPRAVNRFSITHGLSRTSMKPRSSHGSIFTFRRRDLGSETDFADDENSTAGDSESHRTSLLVPWPLRRPSTLGQPSPGTSTPGHVLNGKRNSTVDCNGVVSLLGAGDPEATSPGSHLLHPMKLERPPDTTTPSEEPGRPQTLTPQAPCVDGFEEPGERQRALSAVSVLTSALEELEESQRRCPPCWIRFAQHYLIWECCPLWMSIKQKVKFMVMDPFADLTITMCIVLNTLFMALEHYNMTTEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSEQRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFIKRTTWDFCCVLLQRPPQKPTALASQGQLPGCIATSSPPPQPESEKAPPARKETRFEEGQRPGQGAPGDAEPVCVPIAMAESDTDDPEEDEENSLSTEEESSKQESQPASGSPEALPEPRVWSQVSETTSSGAEASEVQADLRQQRRAEAPAPGCSELPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCTVDTTQAHGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLISLVANALGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSDCESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVGIFTAECIFKMVALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIPKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATIIQRAFRRHLLQRSVKHASFLYRQQAGSSGLSEEDAPEQEGLIAYMMNENFSRRPGPPSSSSVSSTSFPPSYDSVTRATSDNPQVRASDYSPSEDLADFPPTPDRDRESIV

Target ORF information:

RefSeq Version	XM_024982394.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024982394.1

ORF Insert Sequence:

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CGTATGTATC AGAAAATATA AAACTAGGCA ATTTGTCGGC TCTTCGAACT 
TTCAGAGTCC TGAGAGCTCT AAAAACTATT TCAGTTATCC CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGAGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATCTTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCC GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCTGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CACGGCCCTC 
GCCTCCCAGG GCCAGCTGCC AGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCATGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGGAATCCC AGCCGGCGTC CGGCAGCCCA GAGGCCCTCC CAGAGCCGAG GGTCTGGAGC 
CAGGTGTCGG AGACCACCTC CTCTGGGGCC GAGGCCAGTG AGGTTCAGGC AGACTTGCGG 
CAGCAGCGGC GAGCAGAGGC CCCCGCCCCA GGGTGCAGTG AGCTTCCCGA AGACAGTTAC 
TCTGAGGGGA GCACGGCAGA TATGACCAAC ACTGCTGACC TCCTGGAGCA GATCCCTGAC 
CTCGGAGAGG ATGTCAAAGA TCCAGAGGAC TGCTTCACTG AAGGCTGTGT CCGCCGCTGT 
CCCTGCTGCA CCGTGGACAC CACACAGGCC CACGGGAAGG TCTGGTGGAG GCTGCGCAAG 
ACCTGCTACC GCATCGTGGA GCACAGCTGG TTCGAGACGT TCATCATCTT CATGATCCTG 
CTCAGCAGTG GCGCACTGGC CTTTGAGGAC ATCTACCTGG AGGAACGGAA GACCATCAAG 
GTCCTGCTGG AGTACGCCGA CAAGATGTTC ACCTACGTCT TCGTGCTGGA GATGCTCCTC 
AAGTGGGTGG CCTACGGCTT CAAGAAGTAC TTCACCAACG CCTGGTGCTG GCTTGATTTC 
CTCATCGTGG ACGTCTCGCT GATCAGCCTG GTGGCCAACG CCCTGGGCTT TGCTGAGATG 
GGCCCCATCA AGTCACTGCG GACCTTGCGT GCGCTCAGAC CCCTGCGAGC CCTGTCACGA 
TTTGAGGGCA TGAGGGTTGT GGTTAACGCC CTGGTGGGCG CCATCCCATC CATCATGAAC 
GTCCTCCTCG TCTGCCTCAT CTTCTGGCTC ATCTTCAGCA TCATGGGCGT GAACCTCTTC 
GCGGGGAAGT TTGGGAGATG CATCAACCAG ACCGAGGGAG ACCTGCCCTT GAACTATACC 
ATCGTGAACA ACAAGAGCGA CTGTGAGTCT TTCAACGTGA CTGGCGAATT GTACTGGACC 
AAGGTGAAGG TCAACTTTGA CAACGTGGGG GCCGGGTACC TGGCCCTTCT GCAGGTGGCA 
ACATTTAAAG GCTGGATGGA CATCATGTAT GCAGCTGTAG ACTCCAGGGG GTACGAGGAG 
CAGCCCCAGT GGGAATACAA CCTCTACATG TATATCTATT TTGTCATCTT CATCATCTTT 
GGGTCTTTCT TCACCCTGAA CCTGTTCATC GGTGTCATCA TTGACAACTT CAACCAGCAG 
AAGAAAAAGT TAGGGGGCCA GGACATCTTC ATGACAGAGG AGCAGAAGAA GTACTACAAC 
GCCATGAAGA AGCTGGGCTC CAAGAAGCCC CAGAAGCCCA TCCCACGGCC CCTGAACAAG 
TACCAGGGCT TCATATTCGA CATTGTGACC AAGCAGGCCT TCGACGTCAC CATCATGTTT 
CTCATCTGCT TAAACATGGT GACCATGATG GTGGAGACAG ACGACCAGAG CCCCGAGAAG 
GTCAACATCT TGGCCAAGAT CAACCTGCTG TTCGTGGGCA TCTTCACAGC CGAGTGTATC 
TTCAAGATGG TTGCCCTGCG CCACTATTAC TTCACCAACA GCTGGAACAT CTTCGACTTC 
GTGGTTGTCA TCCTCTCCAT CGTAGGCACT GTGCTCTCAG ACATCATCCA GAAGTACTTC 
TTCTCCCCGA CGCTCTTCCG CGTCATCCGC CTGGCCCGCA TAGGCCGCAT CCTCAGGCTG 
ATCCGCGGGG CCAAGGGCAT CCGCACGCTG CTCTTCGCCC TCATGATGTC CCTGCCCGCG 
CTCTTCAACA TCGGGCTGCT GCTCTTCCTC GTCATGTTCA TCTACTCCAT CTTCGGCATG 
GCCAACTTCG CCTACGTCAA GTGGGAGGCT GGCATCGACG ACATGTTCAA CTTCCAGACC 
TTCGCCAACA GCATGCTGTG CCTCTTCCAG ATCACCACGT CGGCGGGCTG GGATGGGCTC 
CTCAGCCCCA TCCTCAACAC GGGGCCCCCC TACTGTGACC CCAACCTGCC CAACAGCAAC 
GGCTCCCGGG GCAACTGCGG GAGCCCTGCG GTGGGCATCC TCTTCTTCAC CACCTACATC 
ATCATCTCCT TCCTCATTGT GGTCAACATG TACATCGCCA TCATCCTGGA GAACTTCAGC 
GTGGCCACGG AGGAGAGCAC GGAGCCCCTG AGTGAGGATG ACTTCGACAT GTTCTACGAG 
ATCTGGGAGA AGTTCGACCC GGAGGCCACC CAGTTCATCG AGTATTTGGC CCTGTCTGAC 
TTCGCCGATG CCCTGTCAGA GCCACTCCGG ATCCCCAAGC CCAACCAGAT AAGCCTCATC 
AATATGGACC TGCCCATGGT GAGTGGAGAC CGCATCCACT GCATGGACAT CCTCTTTGCC 
TTCACCAAGA GGGTCCTGGG CGAATCTGGG GAGATGGACG CCCTGAAGAT CCAGATGGAG 
GAGAAGTTCA TGGCGGCCAA CCCGTCCAAG ATCTCCTACG AGCCCATCAC CACCACGCTG 
CGGCGGAAGC ACGAGGAGGT GTCGGCCACG ATCATCCAGC GGGCCTTCCG CCGGCACCTG 
CTGCAGCGCT CCGTCAAGCA CGCCTCCTTC CTCTACCGCC AGCAGGCGGG CAGCAGCGGC 
CTCTCGGAGG AGGACGCCCC CGAGCAGGAG GGCCTCATCG CCTACATGAT GAACGAGAAC 
TTCTCCCGCC GCCCCGGCCC GCCCTCCAGC TCCTCCGTCT CCTCCACGTC CTTCCCGCCC 
TCCTATGACA GCGTCACCAG GGCCACCAGC GACAACCCCC AGGTGCGGGC GTCTGACTAC 
AGCCCAAGCG AGGATCTCGC AGACTTCCCC CCAACCCCCG ACAGGGACCG TGAGTCAATC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN5A ( NM_174458.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776883.1 Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), mRNA.

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CloneID	OBa40952
Clone ID Related Accession (Same CDS sequence)	NM_174458.2
Accession Version	NM_174458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	sodium channel protein type 5 subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ251721.1. On or before Mar 29, 2005 this sequence version replaced XM_594905.1, NM_174458.1. ##Evidence-Data-START## Transcript exon combination :: AJ251721.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CATACACCAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC TTTACGTACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCAGTCATTT CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGGGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATATTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCT GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCCGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CGCGGCCCTC 
GCCTCCCAGG GCCAGCTGCC GGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCGTGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGCAGGAAT CCCAGCTGGC GTCCGGCAGC CCAGAGGCCC TCCCAGAGCC GAGGGTCTGG 
AGCCAGGTGT CGGAGACCAC CTCCTCTGGG GCCGAGGCCA GTGAGGTTCA GGCAGACTTG 
CGGCAGCAGC GGCGAGCAGA GGCCCCCGCC CCAGGGTGCA GTGAGCTTCC CGAAGACAGT 
TACTCTGAGG GGAGCACGGC AGATATGACC AACACTGCTG ACCTCCTGGA GCAGATCCCT 
GACCTCGGAG AGGATGTCAA AGATCCAGAG GACTGCTTCA CTGAAGGCTG TGTCCGCCGC 
TGTCCCTGCT GCACCGTGGA CACCACACAG GCCCACGGGA AGGTCTGGTG GAGGCTGCGC 
AAGACCTGCT ACCGCATCGT GGAGCACAGC TGGTTCGAGA CGTTCATCAT CTTCATGATC 
CTGCTCAGCA GTGGCGCACT GGCCTTTGAG GACATCTACC TGGAGGAGCG GAAGACCATC 
AAGGTCCTGC TGGAGTACGC CGACAAGATG TTCACCTACG TCTTCGTGCT GGAGATGCTC 
CTCAAGTGGG TGGCCTACGG CTTCAAGAAG TACTTCACCA ACGCCTGGTG CTGGCTTGAT 
TTCCTCATCG TGGACGTCTT GCTGATCAGC CTGGTGGCCA ACGCCCTGGG CTTTGCTGAG 
ATGGGCCCCA TCAAGTCACT GCGGACCTTG CGTGCGCTCA GACCCCTGCG AGCCCTGTCA 
CGATTTGAGG GCATGAGGGT TGTGGTTAAC GCCCTGGTGG GCGCCATCCC ATCCATCATG 
AACGTCCTCC TCGTCTGCCT CATCTTCTGG CTCATCTTCA GCATCATGGG CGTGAACCTC 
TTCGCGGGGA AGTTTGGGAG ATGCATCAAC CAGACCGAGG GAGACCTGCC CTTGAACTAT 
ACCATCGTGA ACAACAAGAG CGACTGTGAG TCTTTCAATG TGACTGGCGA ATTGTACTGG 
ACCAAGGTGA AGGTCAACTT TGACAACGTG GGGGCCGGGT ACCTGGCCCT TCTGCAGGTG 
GCAACATTTA AAGGCTGGAT GGACATCATG TATGCAGCTG TAGACTCCAG GGGGTACGAG 
GAGCAGCCCC AGTGGGAATA CAACCTCTAC ATGTATATCT ATTTTGTCAT CTTCATCATC 
TTTGGGTCTT TCTTCACCCT GAACCTGTTC ATCGGTGTCA TCATTGACAA CTTCAACCAG 
CAGAAGAAAA AGTTAGGGGG CCAGGACATC TTCATGACAG AGGAGCAGAA GAAGTACTAC 
AACGCCATGA AGAAGCTGGG CTCCAAGAAG CCCCAGAAGC CCATCCCACG GCCCCTGAAC 
AAGTACCAGG GCTTCATATT CGACATTGTG ACCAAGCAGG CCTTCGACGT CACCATCATG 
TTTCTCATCT GCTTAAACAT GGTGACCATG ATGGTGGAGA CAGACGACCA GAGCCCCGAG 
AAGGTCAACA TCTTGGCCAA GATCAACCTG CTGTTCGTGG GCATCTTCAC AGCCGAGTGT 
ATCTTCAAGA TGGTTGCCCT GCGCCACTAT TACTTCACCA ACAGCTGGAA CATCTTCGAC 
TTCGTGGTTG TCATCCTCTC CATCGTAGGC ACTGTGCTCT CAGACATCAT CCAGAAGTAC 
TTCTTCTCCC CGACGCTCTT CCGCGTCATC CGCCTGGCCC GCATCAGCCG CATCCTCAGG 
CTGATCCGCG GGGCCAAGGG CATCCGCACG CTTCTCTTCG CCCTCATGAT GTCCCTGCCC 
GCGCTCTTCA ACATCGGGCT GCTGCTCTTC CTCGTCATGT TCATCTACTC CATCTTCGGC 
ATGGCCAACT TCGCCTACGT CAAGTGGGAG GCTGGCATCG ACGACATGTT CAACTTCCAG 
ACCTTCGCCA ACAGCATGCT GTGCCTCTTC CAGATCACCA CGTCGGCGGG CTGGGATGGG 
CTCCTCAGCC CCATCCTCAA CACGGGGCCC CCCTACTGCG ACCCCAACCT GCCCAACAGC 
AACGGCTCCC GGGGCAACTG CGGGAGCCCC GCGGTGGGCA TCCTCTTCTT CACCACCTAC 
ATCATCATCT CCTTCCTCAT TGTGGTCAAC ATGTACATCG CCATCATCCT GGAGAACTTC 
AGCGTGGCCA CGGAGGAGAG CACGGAGCCC CTGAGTGAGG ATGACTTCGA CATGTTCTAC 
GAGATCTGGG AGAAGTTCGA CCCGGAGGCC ACCCAGTTCA TCGAGTATTT GGCCCTGTCT 
GACTTCGCCG ATGCCCTGTC AGAGCCACTC CGGATCCCCA AGCCCAACCA GATAAGCCTC 
ATCAATATGG ACCTGCCCAT GGTGAGTGGA GACCGCATCC ACTGCATGGA CATCCTCTTT 
GCCTTCACCA AGAGGGTCCT GGGCGAATCT GGGGAGATGG ACGCCCTGAA GATCCAGATG 
GAGGAGAAGT TCATGGCGGC CAACCCGTCC AAGATCTCCT ACGAGCCCAT CACCACCACG 
CTGCGGCGGA AGCACGAGGA GGTGTCGGCC ACGATCATCC AGCGGGCCTT CCGCCGGCAC 
CTGCTGCAGC GCTCCGTCAA GCACGCCTCC TTCCTCTACC GCCAGCAGGC GGGCAGCAGC 
GGCCTCTCGG AGGAGGACGC CCCCGAGCAG GAGGGCCTCA TCGCCTACAT GATGAACGAG 
AACTTCTCCC GCCGCCCCGG CCCGCCCTCC AGCTCCTCCG TCTCCTCCAC GTCCTTCCCG 
CCCTCCTACG ACAGCGTCAC CAGGGCCACC AGCGACAACC CCCAGGTGCG GGCGTCTGAC 
TACAGCCCAA GCGAGGATCT CGCCGACTTC CCCCCAACCC CCGACAGGGA CCGTGAGTCA 
ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776883.1
CDS	80..6148
Translation	MAAFLLPRGTSSFRRFTRESLAAIEKRMAEKQARSSAASQESRDGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALHVLSPFHPIRRVAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYIFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTVLNGTNSTNASVEADGLIWASLDDYLNDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGGNPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMELLKKEQEALAIRGVDTVSRSSLEMSPLAPVTTHERRSKRRKRMSSGMEECGDDKFPKSDSEDGPRAVNRFSITHGLSRTSMKPRSSHGSIFTFRRRDLGSETDFADDENSTAGDSESHRTSLLVPWPLRRPSTLGQPSPGTSTPGHVLNGKRNSTVDCNGVVSLLGAGDPEATSPGSHLLHPMKLERPPDTTTPSEEPGRPQTLTPQAPCVDGFEEPGERQRALSAVSVLTSALEELEESQRRCPPCWIRFAQHYLIWECCPLWMSIKQKVKFMVMDPFADLTITMCIVLNTLFMALEHYNMTTEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFCLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSEQRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFIKRTTWDFCCVLLQRPPQKPAALASQGQLPGCIATSSPPPQPESEKAPPARKETRFEEGQRPGQGAPGDAEPVCVPIAVAESDTDDPEEDEENSLSTEEESSKQQESQLASGSPEALPEPRVWSQVSETTSSGAEASEVQADLRQQRRAEAPAPGCSELPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCTVDTTQAHGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVLLISLVANALGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSDCESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVGIFTAECIFKMVALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARISRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDFADALSEPLRIPKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATIIQRAFRRHLLQRSVKHASFLYRQQAGSSGLSEEDAPEQEGLIAYMMNENFSRRPGPPSSSSVSSTSFPPSYDSVTRATSDNPQVRASDYSPSEDLADFPPTPDRDRESIV

Target ORF information:

RefSeq Version	NM_174458.2
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 5 (SCN5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174458.2

ORF Insert Sequence:

ATGGCAGCCT TCCTGTTACC TCGGGGCACC AGCAGCTTCC GCAGGTTCAC CCGGGAGTCT 
CTGGCGGCCA TCGAGAAGCG CATGGCAGAG AAGCAGGCCC GGAGCTCGGC CGCCTCGCAG 
GAGAGCCGCG ACGGGCTGCC CGAGGAGGAG GCGCCCCGGC CCCAGCTGGA CCTGCAAGCC 
TCCAAAAAGC TGCCGGATCT CTACGGCAAC CCACCCCGAG AGCTCATCGG GGAGCCCCTG 
GAGGACCTGG ACCCCTTCTA TAGCACTCAA AAGACCTTCA TCGTCCTGAA CAAAGGCAAG 
ACCATCTTCC GGTTCAGCGC CACCAACGCC TTGCATGTCC TCAGCCCCTT CCACCCCATC 
CGGAGAGTGG CTGTGAAGAT CTTGGTGCAT TCGCTCTTCA GCATGCTCAT CATGTGTACC 
ATTCTGACCA ACTGCGTGTT CATGGCCCAG CACGACCCTC CGCCCTGGAC CAAATATGTC 
GAGTACATCT TCACTGCCAT CTACACCTTT GAGTCTCTGG TCAAGATTCT GGCTCGAGGC 
TTCTGCCTGC ACGCGTTCAC CTTCCTTCGG GACCCGTGGA ACTGGCTGGA CTTCAGCGTG 
ATCATCATGG CATACACCAC TGAATTTGTG GACCTGGGCA ATGTCTCAGC TTTACGTACC 
TTCCGAGTCC TCCGGGCCCT GAAAACTATA TCAGTCATTT CAGGCCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCTGT GAAGAAGCTG GCCGATGTGA TGGTCCTCAC GGTCTTCTGC 
CTCAGCGTCT TCGCCCTCAT CGGCCTTCAG CTCTTCATGG GCAACCTGAG GCACAAGTGC 
GTCCGCAACT TCACGGTGCT CAACGGCACC AACAGCACCA ATGCCTCCGT GGAGGCCGAC 
GGCCTGATCT GGGCATCGCT GGACGACTAC CTCAACGACC CAGAAAATTA CCTACTCAAG 
AATGGCACCT CTGACGTGTT ACTGTGTGGG AACAGCTCCG ACGCTGGGAC ATGTCCTGAG 
GGCTACAGGT GCCTGAAGGC AGGTGGGAAC CCTGACCATG GCTACACCAG CTTCGACTCC 
TTCGCCTGGG CCTTCCTCGC ACTCTTCCGA CTGATGACGC AGGACTGCTG GGAGCGCCTC 
TACCAGCAGA CCCTGAGGTC TGCAGGGAAG ATCTACATGA TCTTCTTCAT GCTGGTCATC 
TTCCTGGGCT CCTTCTACTT GGTGAACTTG ATCCTGGCTG TGGTCGCCAT GGCCTACGAG 
GAGCAAAACC AAGCCACCAT CGCAGAGACA GAGGAGAAGG AAAAGCGATT CCAGGAAGCC 
ATGGAGTTGC TCAAGAAAGA GCAGGAGGCC CTCGCCATCA GGGGTGTGGA CACCGTGTCC 
CGCAGCTCCT TGGAGATGTC CCCATTGGCC CCAGTAACCA CCCACGAGAG AAGGAGCAAG 
AGAAGAAAAC GAATGTCTTC AGGGATGGAA GAGTGTGGGG ACGACAAGTT CCCCAAGTCC 
GACTCAGAGG ATGGTCCCCG AGCAGTGAAT CGTTTCAGCA TCACCCATGG CCTCAGCAGG 
ACCTCCATGA AGCCGCGCTC CAGCCACGGG AGCATTTTCA CCTTCCGCCG ACGGGACCTG 
GGCTCCGAGA CAGATTTTGC GGACGATGAA AACAGCACCG CCGGGGACAG TGAGAGCCAC 
CGCACATCAC TGCTGGTGCC TTGGCCCCTG CGGCGGCCTA GTACCCTGGG ACAGCCCAGT 
CCCGGAACCT CAACTCCCGG CCACGTGCTC AACGGCAAAA GGAACAGCAC TGTGGACTGT 
AACGGGGTGG TCTCCTTGCT GGGGGCAGGA GACCCCGAGG CCACCTCCCC AGGGAGTCAC 
CTCCTCCACC CTATGAAGCT GGAGCGCCCC CCAGACACGA CCACACCATC GGAGGAGCCG 
GGCAGGCCCC AGACGCTGAC GCCCCAGGCT CCGTGTGTAG ACGGCTTCGA GGAGCCAGGA 
GAGCGGCAGC GAGCCCTCAG TGCAGTGAGC GTCCTCACCA GTGCCCTGGA AGAGCTGGAG 
GAGTCTCAGC GCAGGTGTCC ACCGTGCTGG ATCCGTTTTG CCCAGCACTA CCTGATCTGG 
GAGTGCTGCC CGCTGTGGAT GTCCATTAAG CAGAAAGTGA AGTTCATGGT CATGGACCCA 
TTTGCTGACC TCACCATCAC CATGTGCATC GTGCTTAACA CGCTCTTCAT GGCACTGGAG 
CACTACAACA TGACGACCGA ATTTGAGGAG ATGCTGCAGG TTGGAAACCT GGTCTTCACA 
GGAATATTCA CAGCAGAGAT GACCTTCAAG ATCATTGCCT TGGACCCCTA CTACTACTTC 
CAGCAGGGCT GGAACATCTT CGACAGCATC ATCGTCATCC TCAGCCTCAT GGAGCTGGGC 
CTGTCCCGCA TGGGCAATCT GTCGGTGCTT CGCTCCTTCT GCCTGCTTCG GGTCTTCAAG 
CTGGCCAAGT CCTGGCCCAC CCTGAACACA CTCATCAAGA TCATTGGGAA CTCAGTGGGC 
GCGCTAGGCA ACCTGACGTT GGTGCTGGCC ATCATTGTGT TCATCTTCGC TGTGGTGGGC 
ATGCAGCTCT TTGGCAAGAA CTACTCAGAG CAGAGGCACC GTATCAGTGA CTCGGGCCTC 
CTGCCCCGCT GGCACATGAT GGACTTCTTC CATGCCTTCC TCATCATCTT CCGCATCCTC 
TGTGGAGAGT GGATCGAGAC CATGTGGGAC TGCATGGAGG TGTCTGGGCA GTCACTATGC 
CTGCTGGTCT TCCTGCTTGT TATGGTCATT GGTAACCTCG TGGTCCTGAA CCTCTTCCTG 
GCTTTACTGC TCAGCTCCTT CAGCGCAGAC AACCTCACAG CTCCCGACGA GGATGGGGAG 
ATGAACAACC TCCAGCTGGC TCTGGCCCGC ATCCAGCGAG GCCTGCGCTT CATCAAGCGG 
ACCACCTGGG ACTTCTGCTG CGTGCTCCTG CAGCGGCCGC CTCAGAAGCC CGCGGCCCTC 
GCCTCCCAGG GCCAGCTGCC GGGCTGTATC GCCACCTCCA GCCCCCCACC CCAACCAGAG 
AGCGAGAAGG CGCCCCCAGC CCGCAAGGAG ACGCGGTTTG AGGAAGGCCA GCGGCCAGGT 
CAGGGCGCAC CTGGGGATGC CGAGCCTGTG TGTGTGCCCA TCGCCGTGGC CGAGTCAGAC 
ACGGATGACC CCGAGGAGGA TGAGGAGAAC AGCCTAAGCA CAGAGGAAGA GTCCAGCAAG 
CAGCAGGAAT CCCAGCTGGC GTCCGGCAGC CCAGAGGCCC TCCCAGAGCC GAGGGTCTGG 
AGCCAGGTGT CGGAGACCAC CTCCTCTGGG GCCGAGGCCA GTGAGGTTCA GGCAGACTTG 
CGGCAGCAGC GGCGAGCAGA GGCCCCCGCC CCAGGGTGCA GTGAGCTTCC CGAAGACAGT 
TACTCTGAGG GGAGCACGGC AGATATGACC AACACTGCTG ACCTCCTGGA GCAGATCCCT 
GACCTCGGAG AGGATGTCAA AGATCCAGAG GACTGCTTCA CTGAAGGCTG TGTCCGCCGC 
TGTCCCTGCT GCACCGTGGA CACCACACAG GCCCACGGGA AGGTCTGGTG GAGGCTGCGC 
AAGACCTGCT ACCGCATCGT GGAGCACAGC TGGTTCGAGA CGTTCATCAT CTTCATGATC 
CTGCTCAGCA GTGGCGCACT GGCCTTTGAG GACATCTACC TGGAGGAGCG GAAGACCATC 
AAGGTCCTGC TGGAGTACGC CGACAAGATG TTCACCTACG TCTTCGTGCT GGAGATGCTC 
CTCAAGTGGG TGGCCTACGG CTTCAAGAAG TACTTCACCA ACGCCTGGTG CTGGCTTGAT 
TTCCTCATCG TGGACGTCTT GCTGATCAGC CTGGTGGCCA ACGCCCTGGG CTTTGCTGAG 
ATGGGCCCCA TCAAGTCACT GCGGACCTTG CGTGCGCTCA GACCCCTGCG AGCCCTGTCA 
CGATTTGAGG GCATGAGGGT TGTGGTTAAC GCCCTGGTGG GCGCCATCCC ATCCATCATG 
AACGTCCTCC TCGTCTGCCT CATCTTCTGG CTCATCTTCA GCATCATGGG CGTGAACCTC 
TTCGCGGGGA AGTTTGGGAG ATGCATCAAC CAGACCGAGG GAGACCTGCC CTTGAACTAT 
ACCATCGTGA ACAACAAGAG CGACTGTGAG TCTTTCAATG TGACTGGCGA ATTGTACTGG 
ACCAAGGTGA AGGTCAACTT TGACAACGTG GGGGCCGGGT ACCTGGCCCT TCTGCAGGTG 
GCAACATTTA AAGGCTGGAT GGACATCATG TATGCAGCTG TAGACTCCAG GGGGTACGAG 
GAGCAGCCCC AGTGGGAATA CAACCTCTAC ATGTATATCT ATTTTGTCAT CTTCATCATC 
TTTGGGTCTT TCTTCACCCT GAACCTGTTC ATCGGTGTCA TCATTGACAA CTTCAACCAG 
CAGAAGAAAA AGTTAGGGGG CCAGGACATC TTCATGACAG AGGAGCAGAA GAAGTACTAC 
AACGCCATGA AGAAGCTGGG CTCCAAGAAG CCCCAGAAGC CCATCCCACG GCCCCTGAAC 
AAGTACCAGG GCTTCATATT CGACATTGTG ACCAAGCAGG CCTTCGACGT CACCATCATG 
TTTCTCATCT GCTTAAACAT GGTGACCATG ATGGTGGAGA CAGACGACCA GAGCCCCGAG 
AAGGTCAACA TCTTGGCCAA GATCAACCTG CTGTTCGTGG GCATCTTCAC AGCCGAGTGT 
ATCTTCAAGA TGGTTGCCCT GCGCCACTAT TACTTCACCA ACAGCTGGAA CATCTTCGAC 
TTCGTGGTTG TCATCCTCTC CATCGTAGGC ACTGTGCTCT CAGACATCAT CCAGAAGTAC 
TTCTTCTCCC CGACGCTCTT CCGCGTCATC CGCCTGGCCC GCATCAGCCG CATCCTCAGG 
CTGATCCGCG GGGCCAAGGG CATCCGCACG CTTCTCTTCG CCCTCATGAT GTCCCTGCCC 
GCGCTCTTCA ACATCGGGCT GCTGCTCTTC CTCGTCATGT TCATCTACTC CATCTTCGGC 
ATGGCCAACT TCGCCTACGT CAAGTGGGAG GCTGGCATCG ACGACATGTT CAACTTCCAG 
ACCTTCGCCA ACAGCATGCT GTGCCTCTTC CAGATCACCA CGTCGGCGGG CTGGGATGGG 
CTCCTCAGCC CCATCCTCAA CACGGGGCCC CCCTACTGCG ACCCCAACCT GCCCAACAGC 
AACGGCTCCC GGGGCAACTG CGGGAGCCCC GCGGTGGGCA TCCTCTTCTT CACCACCTAC 
ATCATCATCT CCTTCCTCAT TGTGGTCAAC ATGTACATCG CCATCATCCT GGAGAACTTC 
AGCGTGGCCA CGGAGGAGAG CACGGAGCCC CTGAGTGAGG ATGACTTCGA CATGTTCTAC 
GAGATCTGGG AGAAGTTCGA CCCGGAGGCC ACCCAGTTCA TCGAGTATTT GGCCCTGTCT 
GACTTCGCCG ATGCCCTGTC AGAGCCACTC CGGATCCCCA AGCCCAACCA GATAAGCCTC 
ATCAATATGG ACCTGCCCAT GGTGAGTGGA GACCGCATCC ACTGCATGGA CATCCTCTTT 
GCCTTCACCA AGAGGGTCCT GGGCGAATCT GGGGAGATGG ACGCCCTGAA GATCCAGATG 
GAGGAGAAGT TCATGGCGGC CAACCCGTCC AAGATCTCCT ACGAGCCCAT CACCACCACG 
CTGCGGCGGA AGCACGAGGA GGTGTCGGCC ACGATCATCC AGCGGGCCTT CCGCCGGCAC 
CTGCTGCAGC GCTCCGTCAA GCACGCCTCC TTCCTCTACC GCCAGCAGGC GGGCAGCAGC 
GGCCTCTCGG AGGAGGACGC CCCCGAGCAG GAGGGCCTCA TCGCCTACAT GATGAACGAG 
AACTTCTCCC GCCGCCCCGG CCCGCCCTCC AGCTCCTCCG TCTCCTCCAC GTCCTTCCCG 
CCCTCCTACG ACAGCGTCAC CAGGGCCACC AGCGACAACC CCCAGGTGCG GGCGTCTGAC 
TACAGCCCAA GCGAGGATCT CGCCGACTTC CCCCCAACCC CCGACAGGGA CCGTGAGTCA 
ATCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SCN5A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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