ROCK2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROCK2
Entrez Gene ID	282041
Full Name	Rho associated coiled-coil containing protein kinase 2
Synonyms	ROCK-II
General protein information	Preferred Names Rho associated coiled-coil containing protein kinase 2 Names rho-associated protein kinase 2 p164 ROCK-2 rho-associated, coiled-coil-containing protein kinase 2 rho-associated, coiled-coil-containing protein kinase II
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024998323.1	XP_024854091.1	rho-associated protein kinase 2 isoform X1
XM_024998328.1	XP_024854096.1	rho-associated protein kinase 2 isoform X6
XM_024998324.1	XP_024854092.1	rho-associated protein kinase 2 isoform X2
XM_024998325.1	XP_024854093.1	rho-associated protein kinase 2 isoform X3
XM_024998327.1	XP_024854095.1	rho-associated protein kinase 2 isoform X5
NM_174452.2	NP_776877.1	rho-associated protein kinase 2
XM_024998326.1	XP_024854094.1	rho-associated protein kinase 2 isoform X4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rock2	NP_033098.2
Drosophila melanogaster (fruit fly)	rok	NP_536796.2
Caenorhabditis elegans (roundworm)	let-502	NP_491440.1
Pan troglodytes (chimpanzee)	ROCK2	XP_525689.3
Rattus norvegicus (Norway rat)	Rock2	NP_037154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000406	XP_310694.5
Xenopus tropicalis (tropical clawed frog)	rock2	XP_004914525.1
Homo sapiens (human)	ROCK2	NP_004841.2
	ROCK2	XP_001096931.2
Canis lupus familiaris (dog)	ROCK2	XP_540083.4
Danio rerio (zebrafish)	rock2a	NP_777288.1
Bos taurus (cattle)	ROCK2	NP_776877.1
Gallus gallus (chicken)	ROCK2	XP_004935820.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Interaction between ROCK II and nucleophosmin/B23 in the regulation of centrosome duplication.
Ma Z, Kanai M, Kawamura K, Kaibuchi K, Ye K, Fukasawa K
Molecular and cellular biology26(23)9016-34(2006 Dec)

Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase.
Tanaka T, Nishimura D, Wu RC, Amano M, Iso T, Kedes L, Nishida H, Kaibuchi K, Hamamori Y
The Journal of biological chemistry281(22)15320-9(2006 Jun)

RhoA and Rho-kinase dependent and independent signals mediate TGF-beta-induced pulmonary endothelial cytoskeletal reorganization and permeability.
Clements RT, Minnear FL, Singer HA, Keller RS, Vincent PA
American journal of physiology. Lung cellular and molecular physiology288(2)L294-306(2005 Feb)

Rho kinase-induced nuclear translocation of ERK1/ERK2 in smooth muscle cell mitogenesis caused by serotonin.
Liu Y, Suzuki YJ, Day RM, Fanburg BL
Circulation research95(6)579-86(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

nucleus-localized ROCK2 targets p300 for phosphorylation to regulate its acetyltransferase activity
Title: Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase.

RhoA/Rho-kinase pathway is an important component of TGF-beta-induced effects on endothelial myosin light chain phosphorylation, cytoskeletal reorganization, and barrier integrity.
Title: RhoA and Rho-kinase dependent and independent signals mediate TGF-beta-induced pulmonary endothelial cytoskeletal reorganization and permeability.

Rho-kinase analysis of coiled-coil association of the RhoA-binding domain
Title: Parallel coiled-coil association of the RhoA-binding domain in Rho-kinase.

The following ROCK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa220129	XM_024998323.1 Latest version!	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220130	XM_024998324.1 Latest version!	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220131	XM_024998325.1 Latest version!	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220132	XM_024998326.1 Latest version!	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220133	XM_024998327.1 Latest version!	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa220134	XM_024998328.1 Latest version!	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa43936	NM_174452.2 Latest version!	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROCK2 ( XM_024998323.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854091.1 Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa220129
Clone ID Related Accession (Same CDS sequence)	XM_024998323.1
Accession Version	XM_024998323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho-associated protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGTT CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCATTCA CCTACCAACG TTCCTCCACT TCTCTCAATA TTGCCACTAA GCCTTCCAGT 
TCTCATATGC TTCTTGACTC TGATTCTGAC TCAGAAGAAG AATCTGTTCC TTACTTACCT 
ATTTCATCGG AACCTAATGA ATCAAGACTA GAAGGATGGC TTTCATTGCC TGTGCGAAAC 
AACACTAAGA AATTTGGATG GGTTAAAAAG TATGTGATTG TAAGCAGTAA GAAGATCCTT 
TTCTATGACA GTGAGCAAGA TAAAGAACAA TCTAATCCTT ACATGGTTTT AGATATAGAC 
AAGTTATTTC ATGTCCGACC AGTTACACAG ACAGATGTAT ATAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTCCAGAT TCTGTATGCC AACGAAGGAG AAAGTAAGAA GGAACAAGAA 
TTTCCAGTGG AGCCAGTGGG AGAAAAATCA AATTATATTT GCCACAAGGG ACATGAATTT 
ATTCCTACTC TGTATCATTT CCCAACCAAC TGTGAGGCAT GTATGAAGCC ATTGTGGCAC 
ATGTTTAAAC CCCCTCCTGC TTTAGAGTGC CGTCGCTGTC ATATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAGGA AATTATAGCG CCTTGCAAAG TGTATTATGA TATTTCATCG 
GCAAAGAATC TATTGTTATT GGCAAATTCT ACAGAAGAGC AGCAAAAGTG GGTTAGTCGG 
TTAGTGAAAA AAATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCACG GTCATCTCCT 
AGAACGTCAA TGAAAATACA ACAAAACCAG TCTATTCGAC GGCCAAGTCG ACAACTTGCT 
CCAAACAAAC CAAGATTGCT TGATTTGGCA TTTAAAGACT GGGATTGGTC ATTTGATGAT 
GTTGATGGTG GTGATGAGGA TGACGATGTT TTTGATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854091.1
CDS	452..4873
Translation	MSRPPPTGKMPGAPEAVSGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCKENDSIQSRKNEESQEIQKKLYTLEEHLSTEIQAKEELEQKCKSVNTRLEKVAKELEEEITLRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSVLESERRDRTHGSEIINDLQGRISGLEEDVKNGKILLAKVELEKRQLQERFTDLEKEKNNMEIDMTYQLKVIQQSLEQEETEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLSEERTLKQKVENLLLEAEKRCSILDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLLEMKMSLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKEAQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDTEADDGFPVHITQSHTMESMSFTYQRSSTSLNIATKPSSSHMLLDSDSDSEEESVPYLPISSEPNESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGGDEDDDVFDF

Target ORF information:

RefSeq Version	XM_024998323.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998323.1

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGTT CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCATTCA CCTACCAACG TTCCTCCACT TCTCTCAATA TTGCCACTAA GCCTTCCAGT 
TCTCATATGC TTCTTGACTC TGATTCTGAC TCAGAAGAAG AATCTGTTCC TTACTTACCT 
ATTTCATCGG AACCTAATGA ATCAAGACTA GAAGGATGGC TTTCATTGCC TGTGCGAAAC 
AACACTAAGA AATTTGGATG GGTTAAAAAG TATGTGATTG TAAGCAGTAA GAAGATCCTT 
TTCTATGACA GTGAGCAAGA TAAAGAACAA TCTAATCCTT ACATGGTTTT AGATATAGAC 
AAGTTATTTC ATGTCCGACC AGTTACACAG ACAGATGTAT ATAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTCCAGAT TCTGTATGCC AACGAAGGAG AAAGTAAGAA GGAACAAGAA 
TTTCCAGTGG AGCCAGTGGG AGAAAAATCA AATTATATTT GCCACAAGGG ACATGAATTT 
ATTCCTACTC TGTATCATTT CCCAACCAAC TGTGAGGCAT GTATGAAGCC ATTGTGGCAC 
ATGTTTAAAC CCCCTCCTGC TTTAGAGTGC CGTCGCTGTC ATATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAGGA AATTATAGCG CCTTGCAAAG TGTATTATGA TATTTCATCG 
GCAAAGAATC TATTGTTATT GGCAAATTCT ACAGAAGAGC AGCAAAAGTG GGTTAGTCGG 
TTAGTGAAAA AAATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCACG GTCATCTCCT 
AGAACGTCAA TGAAAATACA ACAAAACCAG TCTATTCGAC GGCCAAGTCG ACAACTTGCT 
CCAAACAAAC CAAGATTGCT TGATTTGGCA TTTAAAGACT GGGATTGGTC ATTTGATGAT 
GTTGATGGTG GTGATGAGGA TGACGATGTT TTTGATTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_024998324.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854092.1 Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa220130
Clone ID Related Accession (Same CDS sequence)	XM_024998324.1
Accession Version	XM_024998324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho-associated protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
ATTCAGAAAA AACTGTACAC ATTAGAAGAA CACCTTAGCA CTGAGATTCA GGCCAAAGAG 
GAACTAGAAC AGAAGTGCAA GTCTGTTAAT ACTCGCTTAG AGAAAGTGGC AAAGGAGTTA 
GAAGAAGAGA TTACCTTAAG GAAAAATGTG GAATCAACAT TAAGACAATT AGAAAGAGAA 
AAAGCACTTC TTCAGCACAA AAATGCAGAA TATCAGCGGA AAGCTGATCA TGAAGCAGAC 
AAGAAGCGAA ATTTGGAGAA TGATGTTAAC AGTTTAAAAG ATCAGCTTGA AGATTTGAAA 
AAAAGAAATC AGAACTCTCA GATATCCACT GAGAAAGTGA ATCAACTCCA GAGACAACTG 
GATGAAACCA ATGCTTTGCT GCGAACAGAA TCTGATACTG CAGCCCGGTT AAGGAAAACA 
CAGGCAGAAA GTTCAAAACA GATTCAGCAG CTGGAATCTA ACAATAGAGA TCTACAAGAC 
AAAAATTGCC TGCTGGAGAC TGCCAAGTTA AAACTTGAAA AGGAATTTAT CAATCTTCAG 
TCAGTTCTAG AATCTGAAAG GAGGGACCGA ACCCATGGAT CAGAGATTAT TAATGATTTA 
CAAGGTAGAA TATCTGGCCT AGAAGAAGAT GTAAAGAATG GTAAAATCTT ATTAGCAAAA 
GTAGAGCTGG AGAAGAGACA ACTACAGGAG AGATTTACTG ATTTGGAAAA GGAAAAGAAC 
AACATGGAAA TAGATATGAC ATACCAACTA AAAGTCATAC AGCAAAGTTT AGAACAAGAA 
GAAACTGAAC ATAAGGCTAC AAAAGCACGG CTTGCAGATA AAAACAAGAT TTATGAATCC 
ATTGAAGAAG CTAAATCAGA AGCCATGAAA GAAATGGAGA AAAAGCTCTC GGAGGAAAGA 
ACTTTAAAAC AGAAAGTAGA GAACTTGTTG CTGGAGGCTG AGAAAAGATG CTCTATATTA 
GACTGTGACC TCAAACAGTC ACAGCAGAAA ATAAATGAAC TCCTCAAACA GAAAGATGTG 
CTAAATGAGG ATGTTAGAAA CTTGACATTA AAAATAGAAC AGGAAACTCA GAAGCGCTGC 
CTTACACAAA ATGACTTGAA GATGCAAACA CAGCAAGTTA ACACACTAAA AATGTCAGAA 
AAGCAGTTAA AGCAAGAGAA TAATCATCTC CTAGAAATGA AAATGAGCTT GGAAAAACAG 
AATGCTGAAC TTCGAAAAGA ACGTCAAGAT GCAGATGGAC AGATGAAAGA GCTCCAGGAT 
CAGCTTGAAG CAGAGCAGTA TTTCTCAACC CTCTATAAAA CACAGGTTAG GGAACTTAAG 
GAAGAATGTG AAGAAAAGAC CAAACTTTGT AAAGAATTAC AGCAGAAGAA GCAGGAATTA 
CAGGATGAAA GGGACTCCTT GGCTGCTCAA CTGGAGATTA CCTTAACCAA AGCAGATTCT 
GAGCAACTGG CTCGTTCAAT TGCTGAGGAA CAGTATTCTG ATTTGGAAAA AGAGAAGATC 
ATGAAAGAGC TGGAGATCAA AGAGATGATG GCTCGACACA AACAGGAACT CACCGAAAAA 
GATGCTACTA TTGCGTCTCT TGAAGAAACT AATAGGACAC TAACTAGTGA TGTTGCCAAT 
CTTGCAAATG AGAAAGAAGA ATTAAATAAC AAACTGAAGG AAGCCCAAGA GCAACTATCA 
AGGTTGAAAG ATGAAGAAAT AAGTGCAGCA GCTATTAAAG CACAATTTGA GAAGCAGCTG 
TTAACAGAGA GGACACTCAA AACTCAAGCT GTGAATAAGT TGGCTGAGAT CATGAATCGA 
AAGGAACCTG TTAAGCGTGG TAATGACACA GATGTGCGGA GAAAAGAAAA GGAGAATAGA 
AAGCTACATA TGGAACTTAA ATCTGAACGC GAAAAACTGA CCCAGCAGAT GATCAAGTAT 
CAGAAAGAAC TGAATGAAAT GCAGGCTCAA ATAGCTGAAG AGAGTCAGAT TCGAATTGAA 
CTACAGATGA CACTGGACAG TAAGGACAGT GACATTGAGC AGCTGCGCTC CCAGCTCCAG 
GCCTTGCACA TTGGTTTGGA TAGTTCCAGT ATAGGCAGTG GACCAGGGGA TACTGAAGCT 
GATGACGGTT TTCCAGTTCA TATCACTCAG TCACACACTA TGGAATCCAT GTCATTCACC 
TACCAACGTT CCTCCACTTC TCTCAATATT GCCACTAAGC CTTCCAGTTC TCATATGCTT 
CTTGACTCTG ATTCTGACTC AGAAGAAGAA TCTGTTCCTT ACTTACCTAT TTCATCGGAA 
CCTAATGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTG TGCGAAACAA CACTAAGAAA 
TTTGGATGGG TTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATCCTTTT CTATGACAGT 
GAGCAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA GTTATTTCAT 
GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGAAAT TCCAAGGATA 
TTCCAGATTC TGTATGCCAA CGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG 
CCAGTGGGAG AAAAATCAAA TTATATTTGC CACAAGGGAC ATGAATTTAT TCCTACTCTG 
TATCATTTCC CAACCAACTG TGAGGCATGT ATGAAGCCAT TGTGGCACAT GTTTAAACCC 
CCTCCTGCTT TAGAGTGCCG TCGCTGTCAT ATTAAATGTC ATAAAGATCA CATGGACAAA 
AAGGAGGAAA TTATAGCGCC TTGCAAAGTG TATTATGATA TTTCATCGGC AAAGAATCTA 
TTGTTATTGG CAAATTCTAC AGAAGAGCAG CAAAAGTGGG TTAGTCGGTT AGTGAAAAAA 
ATACCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCTCCTAG AACGTCAATG 
AAAATACAAC AAAACCAGTC TATTCGACGG CCAAGTCGAC AACTTGCTCC AAACAAACCA 
AGATTGCTTG ATTTGGCATT TAAAGACTGG GATTGGTCAT TTGATGATGT TGATGGTGGT 
GATGAGGATG ACGATGTTTT TGATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854092.1
CDS	452..4861
Translation	MSRPPPTGKMPGAPEAVSGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCKENDSIQSRKNEIQKKLYTLEEHLSTEIQAKEELEQKCKSVNTRLEKVAKELEEEITLRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSVLESERRDRTHGSEIINDLQGRISGLEEDVKNGKILLAKVELEKRQLQERFTDLEKEKNNMEIDMTYQLKVIQQSLEQEETEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLSEERTLKQKVENLLLEAEKRCSILDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLLEMKMSLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKEAQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDTEADDGFPVHITQSHTMESMSFTYQRSSTSLNIATKPSSSHMLLDSDSDSEEESVPYLPISSEPNESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGGDEDDDVFDF

Target ORF information:

RefSeq Version	XM_024998324.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998324.1

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
ATTCAGAAAA AACTGTACAC ATTAGAAGAA CACCTTAGCA CTGAGATTCA GGCCAAAGAG 
GAACTAGAAC AGAAGTGCAA GTCTGTTAAT ACTCGCTTAG AGAAAGTGGC AAAGGAGTTA 
GAAGAAGAGA TTACCTTAAG GAAAAATGTG GAATCAACAT TAAGACAATT AGAAAGAGAA 
AAAGCACTTC TTCAGCACAA AAATGCAGAA TATCAGCGGA AAGCTGATCA TGAAGCAGAC 
AAGAAGCGAA ATTTGGAGAA TGATGTTAAC AGTTTAAAAG ATCAGCTTGA AGATTTGAAA 
AAAAGAAATC AGAACTCTCA GATATCCACT GAGAAAGTGA ATCAACTCCA GAGACAACTG 
GATGAAACCA ATGCTTTGCT GCGAACAGAA TCTGATACTG CAGCCCGGTT AAGGAAAACA 
CAGGCAGAAA GTTCAAAACA GATTCAGCAG CTGGAATCTA ACAATAGAGA TCTACAAGAC 
AAAAATTGCC TGCTGGAGAC TGCCAAGTTA AAACTTGAAA AGGAATTTAT CAATCTTCAG 
TCAGTTCTAG AATCTGAAAG GAGGGACCGA ACCCATGGAT CAGAGATTAT TAATGATTTA 
CAAGGTAGAA TATCTGGCCT AGAAGAAGAT GTAAAGAATG GTAAAATCTT ATTAGCAAAA 
GTAGAGCTGG AGAAGAGACA ACTACAGGAG AGATTTACTG ATTTGGAAAA GGAAAAGAAC 
AACATGGAAA TAGATATGAC ATACCAACTA AAAGTCATAC AGCAAAGTTT AGAACAAGAA 
GAAACTGAAC ATAAGGCTAC AAAAGCACGG CTTGCAGATA AAAACAAGAT TTATGAATCC 
ATTGAAGAAG CTAAATCAGA AGCCATGAAA GAAATGGAGA AAAAGCTCTC GGAGGAAAGA 
ACTTTAAAAC AGAAAGTAGA GAACTTGTTG CTGGAGGCTG AGAAAAGATG CTCTATATTA 
GACTGTGACC TCAAACAGTC ACAGCAGAAA ATAAATGAAC TCCTCAAACA GAAAGATGTG 
CTAAATGAGG ATGTTAGAAA CTTGACATTA AAAATAGAAC AGGAAACTCA GAAGCGCTGC 
CTTACACAAA ATGACTTGAA GATGCAAACA CAGCAAGTTA ACACACTAAA AATGTCAGAA 
AAGCAGTTAA AGCAAGAGAA TAATCATCTC CTAGAAATGA AAATGAGCTT GGAAAAACAG 
AATGCTGAAC TTCGAAAAGA ACGTCAAGAT GCAGATGGAC AGATGAAAGA GCTCCAGGAT 
CAGCTTGAAG CAGAGCAGTA TTTCTCAACC CTCTATAAAA CACAGGTTAG GGAACTTAAG 
GAAGAATGTG AAGAAAAGAC CAAACTTTGT AAAGAATTAC AGCAGAAGAA GCAGGAATTA 
CAGGATGAAA GGGACTCCTT GGCTGCTCAA CTGGAGATTA CCTTAACCAA AGCAGATTCT 
GAGCAACTGG CTCGTTCAAT TGCTGAGGAA CAGTATTCTG ATTTGGAAAA AGAGAAGATC 
ATGAAAGAGC TGGAGATCAA AGAGATGATG GCTCGACACA AACAGGAACT CACCGAAAAA 
GATGCTACTA TTGCGTCTCT TGAAGAAACT AATAGGACAC TAACTAGTGA TGTTGCCAAT 
CTTGCAAATG AGAAAGAAGA ATTAAATAAC AAACTGAAGG AAGCCCAAGA GCAACTATCA 
AGGTTGAAAG ATGAAGAAAT AAGTGCAGCA GCTATTAAAG CACAATTTGA GAAGCAGCTG 
TTAACAGAGA GGACACTCAA AACTCAAGCT GTGAATAAGT TGGCTGAGAT CATGAATCGA 
AAGGAACCTG TTAAGCGTGG TAATGACACA GATGTGCGGA GAAAAGAAAA GGAGAATAGA 
AAGCTACATA TGGAACTTAA ATCTGAACGC GAAAAACTGA CCCAGCAGAT GATCAAGTAT 
CAGAAAGAAC TGAATGAAAT GCAGGCTCAA ATAGCTGAAG AGAGTCAGAT TCGAATTGAA 
CTACAGATGA CACTGGACAG TAAGGACAGT GACATTGAGC AGCTGCGCTC CCAGCTCCAG 
GCCTTGCACA TTGGTTTGGA TAGTTCCAGT ATAGGCAGTG GACCAGGGGA TACTGAAGCT 
GATGACGGTT TTCCAGTTCA TATCACTCAG TCACACACTA TGGAATCCAT GTCATTCACC 
TACCAACGTT CCTCCACTTC TCTCAATATT GCCACTAAGC CTTCCAGTTC TCATATGCTT 
CTTGACTCTG ATTCTGACTC AGAAGAAGAA TCTGTTCCTT ACTTACCTAT TTCATCGGAA 
CCTAATGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTG TGCGAAACAA CACTAAGAAA 
TTTGGATGGG TTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATCCTTTT CTATGACAGT 
GAGCAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA GTTATTTCAT 
GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGAAAT TCCAAGGATA 
TTCCAGATTC TGTATGCCAA CGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG 
CCAGTGGGAG AAAAATCAAA TTATATTTGC CACAAGGGAC ATGAATTTAT TCCTACTCTG 
TATCATTTCC CAACCAACTG TGAGGCATGT ATGAAGCCAT TGTGGCACAT GTTTAAACCC 
CCTCCTGCTT TAGAGTGCCG TCGCTGTCAT ATTAAATGTC ATAAAGATCA CATGGACAAA 
AAGGAGGAAA TTATAGCGCC TTGCAAAGTG TATTATGATA TTTCATCGGC AAAGAATCTA 
TTGTTATTGG CAAATTCTAC AGAAGAGCAG CAAAAGTGGG TTAGTCGGTT AGTGAAAAAA 
ATACCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCTCCTAG AACGTCAATG 
AAAATACAAC AAAACCAGTC TATTCGACGG CCAAGTCGAC AACTTGCTCC AAACAAACCA 
AGATTGCTTG ATTTGGCATT TAAAGACTGG GATTGGTCAT TTGATGATGT TGATGGTGGT 
GATGAGGATG ACGATGTTTT TGATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_024998325.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854093.1 Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa220131
Clone ID Related Accession (Same CDS sequence)	XM_024998325.1
Accession Version	XM_024998325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho-associated protein kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGTT CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCATTCA CCTACCAACG TTCCTCCACT TCTCTCAATA TTGCCACTAA GCCTTCCAGT 
TCTCATATGC TTCTTGACTC TGATTCTGAC TCAGAAGAAG AATCTGTTCC TTACTTACCT 
ATTTCATCGG AACCTAATGA ATCAAGACTA GAAGGATGGC TTTCATTGCC TGTGCGAAAC 
AACACTAAGA AATTTGGATG GGTTAAAAAG TATGTGATTG TAAGCAGTAA GAAGATCCTT 
TTCTATGACA GTGAGCAAGA TAAAGAACAA TCTAATCCTT ACATGGTTTT AGATATAGAC 
AAGTTATTTC ATGTCCGACC AGTTACACAG ACAGATGTAT ATAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTCCAGAT TCTGTATGCC AACGAAGGAG AAAGTAAGAA GGAACAAGAA 
TTTCCAGTGG AGCCAGTGGG AGAAAAATCA AATTATATTT GCCACAAGGG ACATGAATTT 
ATTCCTACTC TGTATCATTT CCCAACCAAC TGTGAGGCAT GTATGAAGCC ATTGTGGCAC 
ATGTTTAAAC CCCCTCCTGC TTTAGAGTGC CGTCGCTGTC ATATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAGGA AATTATAGCG CCTTGCAAAG TGTATTATGA TATTTCATCG 
GCAAAGAATC TATTGTTATT GGCAAATTCT ACAGAAGAGC AGCAAAAGTG GGTTAGTCGG 
TTAGTGAAAA AAATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCACG GTCATCTCCT 
AGAACGTCAA TGAAAATACA ACAAAACCAG TCTATTCGAC GGCCAAGTCG ACAACTTGCT 
CCAAACAAAC CAAGTTCTGG CCCTGCCCTT CGAATCATTC TTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854093.1
CDS	1..4368
Translation	MSRPPPTGKMPGAPEAVSGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCKENDSIQSRKNEESQEIQKKLYTLEEHLSTEIQAKEELEQKCKSVNTRLEKVAKELEEEITLRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSVLESERRDRTHGSEIINDLQGRISGLEEDVKNGKILLAKVELEKRQLQERFTDLEKEKNNMEIDMTYQLKVIQQSLEQEETEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLSEERTLKQKVENLLLEAEKRCSILDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLLEMKMSLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKEAQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDTEADDGFPVHITQSHTMESMSFTYQRSSTSLNIATKPSSSHMLLDSDSDSEEESVPYLPISSEPNESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPSSGPALRIILH

Target ORF information:

RefSeq Version	XM_024998325.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998325.1

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGTT CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCATTCA CCTACCAACG TTCCTCCACT TCTCTCAATA TTGCCACTAA GCCTTCCAGT 
TCTCATATGC TTCTTGACTC TGATTCTGAC TCAGAAGAAG AATCTGTTCC TTACTTACCT 
ATTTCATCGG AACCTAATGA ATCAAGACTA GAAGGATGGC TTTCATTGCC TGTGCGAAAC 
AACACTAAGA AATTTGGATG GGTTAAAAAG TATGTGATTG TAAGCAGTAA GAAGATCCTT 
TTCTATGACA GTGAGCAAGA TAAAGAACAA TCTAATCCTT ACATGGTTTT AGATATAGAC 
AAGTTATTTC ATGTCCGACC AGTTACACAG ACAGATGTAT ATAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTCCAGAT TCTGTATGCC AACGAAGGAG AAAGTAAGAA GGAACAAGAA 
TTTCCAGTGG AGCCAGTGGG AGAAAAATCA AATTATATTT GCCACAAGGG ACATGAATTT 
ATTCCTACTC TGTATCATTT CCCAACCAAC TGTGAGGCAT GTATGAAGCC ATTGTGGCAC 
ATGTTTAAAC CCCCTCCTGC TTTAGAGTGC CGTCGCTGTC ATATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAGGA AATTATAGCG CCTTGCAAAG TGTATTATGA TATTTCATCG 
GCAAAGAATC TATTGTTATT GGCAAATTCT ACAGAAGAGC AGCAAAAGTG GGTTAGTCGG 
TTAGTGAAAA AAATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCACG GTCATCTCCT 
AGAACGTCAA TGAAAATACA ACAAAACCAG TCTATTCGAC GGCCAAGTCG ACAACTTGCT 
CCAAACAAAC CAAGTTCTGG CCCTGCCCTT CGAATCATTC TTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_024998326.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854094.1 Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

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CloneID	OBa220132
Clone ID Related Accession (Same CDS sequence)	XM_024998326.1
Accession Version	XM_024998326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho-associated protein kinase 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGTT CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCATTCA CCTACCAACG TTCCTCCACT TCTCTCAATA TTGCCACTAA GCCTTCCAGT 
TCTCATATGC TTCTTGACTC TGATTCTGAC TCAGAAGAAG AATCTGTTCC TTACTTACCT 
ATTTCATCGG AACCTAATGA ATCAAGACTA GAAGGATGGC TTTCATTGCC TGTGCGAAAC 
AACACTAAGA AATTTGGATG GGTTAAAAAG TATGTGATTG TAAGCAGTAA GAAGATCCTT 
TTCTATGACA GTGAGCAAGA TAAAGAACAA TCTAATCCTT ACATGGTTTT AGATATAGAC 
AAGTTATTTC ATGTCCGACC AGTTACACAG ACAGATGTAT ATAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTCCAGAT TCTGTATGCC AACGAAGGAG AAAGTAAGAA GGAACAAGAA 
TTTCCAGTGG AGCCAGTGGG AGAAAAATCA AATTATATTT GCCACAAGGG ACATGAATTT 
ATTCCTACTC TGTATCATTT CCCAACCAAC TGTGAGGCAT GTATGAAGCC ATTGTGGCAC 
ATGTTTAAAC CCCCTCCTGC TTTAGAGTGC CGTCGCTGTC ATATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAGGA AATTATAGCG CCTTGCAAAG TGTATTATGA TATTTCATCG 
GCAAAGAATC TATTGTTATT GGCAAATTCT ACAGAAGAGC AGCAAAAGTG GGTTAGTCGG 
TTAGTGAAAA AAATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCACG GTCATCTCCT 
AGAACGTCAA TGAAAATACA ACAAAACCAG TCTATTCGAC GGCCAAGTCG ACAACTTGCT 
CCAAACAAAC CAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854094.1
CDS	1..4338
Translation	MSRPPPTGKMPGAPEAVSGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCKENDSIQSRKNEESQEIQKKLYTLEEHLSTEIQAKEELEQKCKSVNTRLEKVAKELEEEITLRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSVLESERRDRTHGSEIINDLQGRISGLEEDVKNGKILLAKVELEKRQLQERFTDLEKEKNNMEIDMTYQLKVIQQSLEQEETEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLSEERTLKQKVENLLLEAEKRCSILDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLLEMKMSLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKEAQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDTEADDGFPVHITQSHTMESMSFTYQRSSTSLNIATKPSSSHMLLDSDSDSEEESVPYLPISSEPNESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	XM_024998326.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998326.1

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGTT CATATCACTC AGTCACACAC TATGGAATCC 
ATGTCATTCA CCTACCAACG TTCCTCCACT TCTCTCAATA TTGCCACTAA GCCTTCCAGT 
TCTCATATGC TTCTTGACTC TGATTCTGAC TCAGAAGAAG AATCTGTTCC TTACTTACCT 
ATTTCATCGG AACCTAATGA ATCAAGACTA GAAGGATGGC TTTCATTGCC TGTGCGAAAC 
AACACTAAGA AATTTGGATG GGTTAAAAAG TATGTGATTG TAAGCAGTAA GAAGATCCTT 
TTCTATGACA GTGAGCAAGA TAAAGAACAA TCTAATCCTT ACATGGTTTT AGATATAGAC 
AAGTTATTTC ATGTCCGACC AGTTACACAG ACAGATGTAT ATAGAGCAGA TGCTAAAGAA 
ATTCCAAGGA TATTCCAGAT TCTGTATGCC AACGAAGGAG AAAGTAAGAA GGAACAAGAA 
TTTCCAGTGG AGCCAGTGGG AGAAAAATCA AATTATATTT GCCACAAGGG ACATGAATTT 
ATTCCTACTC TGTATCATTT CCCAACCAAC TGTGAGGCAT GTATGAAGCC ATTGTGGCAC 
ATGTTTAAAC CCCCTCCTGC TTTAGAGTGC CGTCGCTGTC ATATTAAATG TCATAAAGAT 
CACATGGACA AAAAGGAGGA AATTATAGCG CCTTGCAAAG TGTATTATGA TATTTCATCG 
GCAAAGAATC TATTGTTATT GGCAAATTCT ACAGAAGAGC AGCAAAAGTG GGTTAGTCGG 
TTAGTGAAAA AAATACCTAA AAAGCCTCCA GCTCCAGACC CTTTTGCACG GTCATCTCCT 
AGAACGTCAA TGAAAATACA ACAAAACCAG TCTATTCGAC GGCCAAGTCG ACAACTTGCT 
CCAAACAAAC CAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_024998327.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854095.1 Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa220133
Clone ID Related Accession (Same CDS sequence)	XM_024998327.1
Accession Version	XM_024998327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho-associated protein kinase 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGAA TCAAGACTAG AAGGATGGCT TTCATTGCCT 
GTGCGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATCCTTT TCTATGACAG TGAGCAAGAT AAAGAACAAT CTAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTATA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ACGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTGGGA GAAAAATCAA ATTATATTTG CCACAAGGGA 
CATGAATTTA TTCCTACTCT GTATCATTTC CCAACCAACT GTGAGGCATG TATGAAGCCA 
TTGTGGCACA TGTTTAAACC CCCTCCTGCT TTAGAGTGCC GTCGCTGTCA TATTAAATGT 
CATAAAGATC ACATGGACAA AAAGGAGGAA ATTATAGCGC CTTGCAAAGT GTATTATGAT 
ATTTCATCGG CAAAGAATCT ATTGTTATTG GCAAATTCTA CAGAAGAGCA GCAAAAGTGG 
GTTAGTCGGT TAGTGAAAAA AATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCACGG 
TCATCTCCTA GAACGTCAAT GAAAATACAA CAAAACCAGT CTATTCGACG GCCAAGTCGA 
CAACTTGCTC CAAACAAACC AAGATTGCTT GATTTGGCAT TTAAAGACTG GGATTGGTCA 
TTTGATGATG TTGATGGTGG TGATGAGGAT GACGATGTTT TTGATTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854095.1
CDS	1..4251
Translation	MSRPPPTGKMPGAPEAVSGDGAGASRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCKENDSIQSRKNEESQEIQKKLYTLEEHLSTEIQAKEELEQKCKSVNTRLEKVAKELEEEITLRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSVLESERRDRTHGSEIINDLQGRISGLEEDVKNGKILLAKVELEKRQLQERFTDLEKEKNNMEIDMTYQLKVIQQSLEQEETEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLSEERTLKQKVENLLLEAEKRCSILDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLLEMKMSLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKEAQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDTEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGGDEDDDVFDF

Target ORF information:

RefSeq Version	XM_024998327.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998327.1

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATTCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGAA TCAAGACTAG AAGGATGGCT TTCATTGCCT 
GTGCGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATCCTTT TCTATGACAG TGAGCAAGAT AAAGAACAAT CTAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTATA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ACGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTGGGA GAAAAATCAA ATTATATTTG CCACAAGGGA 
CATGAATTTA TTCCTACTCT GTATCATTTC CCAACCAACT GTGAGGCATG TATGAAGCCA 
TTGTGGCACA TGTTTAAACC CCCTCCTGCT TTAGAGTGCC GTCGCTGTCA TATTAAATGT 
CATAAAGATC ACATGGACAA AAAGGAGGAA ATTATAGCGC CTTGCAAAGT GTATTATGAT 
ATTTCATCGG CAAAGAATCT ATTGTTATTG GCAAATTCTA CAGAAGAGCA GCAAAAGTGG 
GTTAGTCGGT TAGTGAAAAA AATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCACGG 
TCATCTCCTA GAACGTCAAT GAAAATACAA CAAAACCAGT CTATTCGACG GCCAAGTCGA 
CAACTTGCTC CAAACAAACC AAGATTGCTT GATTTGGCAT TTAAAGACTG GGATTGGTCA 
TTTGATGATG TTGATGGTGG TGATGAGGAT GACGATGTTT TTGATTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( XM_024998328.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024854096.1 Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa220134
Clone ID Related Accession (Same CDS sequence)	XM_024998328.1
Accession Version	XM_024998328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho-associated protein kinase 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTCA AAAATAGACG TTGTCCTTGC CCTTATGGAA TTAGCAGTCT AATGAAAAAG 
GTTCGTCATA AGGCATCACA GAAAGTTTAT GCTATGAAGC TTCTTAGTAA GTTTGAAATG 
ATAAAAAGAT CAGATTCTGC TTTTTTCTGG GAGGAAAGAG ATATTATGGC CTTTGCCAAC 
AGTCCCTGGG TGGTTCAGCT CTTTTGTGCC TTTCAAGATG ATAAGTATCT GTACATGGTA 
ATGGAGTACA TGCCTGGTGG AGACCTTGTA AACCTTATGA GTAACTATGA TGTACCTGAA 
AAATGGGCCA AATTTTATAC TGCTGAAGTT GTTCTTGCTT TGGATGCCAT ACACTCCATG 
GGTTTAATTC ACAGAGATGT GAAGCCTGAC AACATGCTCT TGGATAAACA TGGGCATCTA 
AAATTAGCAG ATTTTGGCAC ATGTATGAAG ATGGATGAAA CAGGCATGGT GCATTGTGAT 
ACAGCAGTTG GAACACCCGA TTATATATCA CCCGAGGTCC TGAAATCACA AGGGGGTGAT 
GGTTACTATG GGCGAGAATG TGATTGGTGG TCCGTGGGTG TTTTCCTTTT TGAAATGCTG 
GTGGGGGATA CTCCATTTTA TGCAGATTCA CTTGTAGGAA CATATAGCAA AATTATGGAT 
CATAAAAACT CACTATGTTT CCCTGAAGAT GCAGAAATTT CTAAACATGC GAAGAATCTC 
ATCTGTGCCT TCTTAACAGA TAGGGAGGTA CGCCTTGGAA GAAACGGGGT AGAAGAAATC 
AAACAACATC CTTTCTTTAA GAATGATCAG TGGAATTGGG ATAACATAAG AGAGACTGCA 
GCTCCTGTGG TACCTGAACT CAGCAGTGAC ATAGACAGCA GCAATTTTGA TGACATTGAA 
GATGATAAAG GAGATGTAGA AACCTTCCCA ATTCCCAAGG CTTTTGTGGG AAATCAGCTA 
CCTTTTATAG GATTTACCTA CTACAGAGAA AATTTGCTAC TAAGTGACTC TCCATCTTGT 
AAAGAAAATG ACTCAATTCA ATCAAGGAAG AATGAAGAGA GTCAAGAGAT TCAGAAAAAA 
CTGTACACAT TAGAAGAACA CCTTAGCACT GAGATTCAGG CCAAAGAGGA ACTAGAACAG 
AAGTGCAAGT CTGTTAATAC TCGCTTAGAG AAAGTGGCAA AGGAGTTAGA AGAAGAGATT 
ACCTTAAGGA AAAATGTGGA ATCAACATTA AGACAATTAG AAAGAGAAAA AGCACTTCTT 
CAGCACAAAA ATGCAGAATA TCAGCGGAAA GCTGATCATG AAGCAGACAA GAAGCGAAAT 
TTGGAGAATG ATGTTAACAG TTTAAAAGAT CAGCTTGAAG ATTTGAAAAA AAGAAATCAG 
AACTCTCAGA TATCCACTGA GAAAGTGAAT CAACTCCAGA GACAACTGGA TGAAACCAAT 
GCTTTGCTGC GAACAGAATC TGATACTGCA GCCCGGTTAA GGAAAACACA GGCAGAAAGT 
TCAAAACAGA TTCAGCAGCT GGAATCTAAC AATAGAGATC TACAAGACAA AAATTGCCTG 
CTGGAGACTG CCAAGTTAAA ACTTGAAAAG GAATTTATCA ATCTTCAGTC AGTTCTAGAA 
TCTGAAAGGA GGGACCGAAC CCATGGATCA GAGATTATTA ATGATTTACA AGGTAGAATA 
TCTGGCCTAG AAGAAGATGT AAAGAATGGT AAAATCTTAT TAGCAAAAGT AGAGCTGGAG 
AAGAGACAAC TACAGGAGAG ATTTACTGAT TTGGAAAAGG AAAAGAACAA CATGGAAATA 
GATATGACAT ACCAACTAAA AGTCATACAG CAAAGTTTAG AACAAGAAGA AACTGAACAT 
AAGGCTACAA AAGCACGGCT TGCAGATAAA AACAAGATTT ATGAATCCAT TGAAGAAGCT 
AAATCAGAAG CCATGAAAGA AATGGAGAAA AAGCTCTCGG AGGAAAGAAC TTTAAAACAG 
AAAGTAGAGA ACTTGTTGCT GGAGGCTGAG AAAAGATGCT CTATATTAGA CTGTGACCTC 
AAACAGTCAC AGCAGAAAAT AAATGAACTC CTCAAACAGA AAGATGTGCT AAATGAGGAT 
GTTAGAAACT TGACATTAAA AATAGAACAG GAAACTCAGA AGCGCTGCCT TACACAAAAT 
GACTTGAAGA TGCAAACACA GCAAGTTAAC ACACTAAAAA TGTCAGAAAA GCAGTTAAAG 
CAAGAGAATA ATCATCTCCT AGAAATGAAA ATGAGCTTGG AAAAACAGAA TGCTGAACTT 
CGAAAAGAAC GTCAAGATGC AGATGGACAG ATGAAAGAGC TCCAGGATCA GCTTGAAGCA 
GAGCAGTATT TCTCAACCCT CTATAAAACA CAGGTTAGGG AACTTAAGGA AGAATGTGAA 
GAAAAGACCA AACTTTGTAA AGAATTACAG CAGAAGAAGC AGGAATTACA GGATGAAAGG 
GACTCCTTGG CTGCTCAACT GGAGATTACC TTAACCAAAG CAGATTCTGA GCAACTGGCT 
CGTTCAATTG CTGAGGAACA GTATTCTGAT TTGGAAAAAG AGAAGATCAT GAAAGAGCTG 
GAGATCAAAG AGATGATGGC TCGACACAAA CAGGAACTCA CCGAAAAAGA TGCTACTATT 
GCGTCTCTTG AAGAAACTAA TAGGACACTA ACTAGTGATG TTGCCAATCT TGCAAATGAG 
AAAGAAGAAT TAAATAACAA ACTGAAGGAA GCCCAAGAGC AACTATCAAG GTTGAAAGAT 
GAAGAAATAA GTGCAGCAGC TATTAAAGCA CAATTTGAGA AGCAGCTGTT AACAGAGAGG 
ACACTCAAAA CTCAAGCTGT GAATAAGTTG GCTGAGATCA TGAATCGAAA GGAACCTGTT 
AAGCGTGGTA ATGACACAGA TGTGCGGAGA AAAGAAAAGG AGAATAGAAA GCTACATATG 
GAACTTAAAT CTGAACGCGA AAAACTGACC CAGCAGATGA TCAAGTATCA GAAAGAACTG 
AATGAAATGC AGGCTCAAAT AGCTGAAGAG AGTCAGATTC GAATTGAACT ACAGATGACA 
CTGGACAGTA AGGACAGTGA CATTGAGCAG CTGCGCTCCC AGCTCCAGGC CTTGCACATT 
GGTTTGGATA GTTCCAGTAT AGGCAGTGGA CCAGGGGATA CTGAAGCTGA TGACGGTTTT 
CCAGTTCATA TCACTCAGTC ACACACTATG GAATCCATGT CATTCACCTA CCAACGTTCC 
TCCACTTCTC TCAATATTGC CACTAAGCCT TCCAGTTCTC ATATGCTTCT TGACTCTGAT 
TCTGACTCAG AAGAAGAATC TGTTCCTTAC TTACCTATTT CATCGGAACC TAATGAATCA 
AGACTAGAAG GATGGCTTTC ATTGCCTGTG CGAAACAACA CTAAGAAATT TGGATGGGTT 
AAAAAGTATG TGATTGTAAG CAGTAAGAAG ATCCTTTTCT ATGACAGTGA GCAAGATAAA 
GAACAATCTA ATCCTTACAT GGTTTTAGAT ATAGACAAGT TATTTCATGT CCGACCAGTT 
ACACAGACAG ATGTATATAG AGCAGATGCT AAAGAAATTC CAAGGATATT CCAGATTCTG 
TATGCCAACG AAGGAGAAAG TAAGAAGGAA CAAGAATTTC CAGTGGAGCC AGTGGGAGAA 
AAATCAAATT ATATTTGCCA CAAGGGACAT GAATTTATTC CTACTCTGTA TCATTTCCCA 
ACCAACTGTG AGGCATGTAT GAAGCCATTG TGGCACATGT TTAAACCCCC TCCTGCTTTA 
GAGTGCCGTC GCTGTCATAT TAAATGTCAT AAAGATCACA TGGACAAAAA GGAGGAAATT 
ATAGCGCCTT GCAAAGTGTA TTATGATATT TCATCGGCAA AGAATCTATT GTTATTGGCA 
AATTCTACAG AAGAGCAGCA AAAGTGGGTT AGTCGGTTAG TGAAAAAAAT ACCTAAAAAG 
CCTCCAGCTC CAGACCCTTT TGCACGGTCA TCTCCTAGAA CGTCAATGAA AATACAACAA 
AACCAGTCTA TTCGACGGCC AAGTCGACAA CTTGCTCCAA ACAAACCAAG ATTGCTTGAT 
TTGGCATTTA AAGACTGGGA TTGGTCATTT GATGATGTTG ATGGTGGTGA TGAGGATGAC 
GATGTTTTTG ATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024854096.1
CDS	109..4266
Translation	MMVKNRRCPCPYGISSLMKKVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCKENDSIQSRKNEESQEIQKKLYTLEEHLSTEIQAKEELEQKCKSVNTRLEKVAKELEEEITLRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSVLESERRDRTHGSEIINDLQGRISGLEEDVKNGKILLAKVELEKRQLQERFTDLEKEKNNMEIDMTYQLKVIQQSLEQEETEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLSEERTLKQKVENLLLEAEKRCSILDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLLEMKMSLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKEAQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDTEADDGFPVHITQSHTMESMSFTYQRSSTSLNIATKPSSSHMLLDSDSDSEEESVPYLPISSEPNESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPRLLDLAFKDWDWSFDDVDGGDEDDDVFDF

Target ORF information:

RefSeq Version	XM_024998328.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024998328.1

ORF Insert Sequence:

ATGATGGTCA AAAATAGACG TTGTCCTTGC CCTTATGGAA TTAGCAGTCT AATGAAAAAG 
GTTCGTCATA AGGCATCACA GAAAGTTTAT GCTATGAAGC TTCTTAGTAA GTTTGAAATG 
ATAAAAAGAT CAGATTCTGC TTTTTTCTGG GAGGAAAGAG ATATTATGGC CTTTGCCAAC 
AGTCCCTGGG TGGTTCAGCT CTTTTGTGCC TTTCAAGATG ATAAGTATCT GTACATGGTA 
ATGGAGTACA TGCCTGGTGG AGACCTTGTA AACCTTATGA GTAACTATGA TGTACCTGAA 
AAATGGGCCA AATTTTATAC TGCTGAAGTT GTTCTTGCTT TGGATGCCAT ACACTCCATG 
GGTTTAATTC ACAGAGATGT GAAGCCTGAC AACATGCTCT TGGATAAACA TGGGCATCTA 
AAATTAGCAG ATTTTGGCAC ATGTATGAAG ATGGATGAAA CAGGCATGGT GCATTGTGAT 
ACAGCAGTTG GAACACCCGA TTATATATCA CCCGAGGTCC TGAAATCACA AGGGGGTGAT 
GGTTACTATG GGCGAGAATG TGATTGGTGG TCCGTGGGTG TTTTCCTTTT TGAAATGCTG 
GTGGGGGATA CTCCATTTTA TGCAGATTCA CTTGTAGGAA CATATAGCAA AATTATGGAT 
CATAAAAACT CACTATGTTT CCCTGAAGAT GCAGAAATTT CTAAACATGC GAAGAATCTC 
ATCTGTGCCT TCTTAACAGA TAGGGAGGTA CGCCTTGGAA GAAACGGGGT AGAAGAAATC 
AAACAACATC CTTTCTTTAA GAATGATCAG TGGAATTGGG ATAACATAAG AGAGACTGCA 
GCTCCTGTGG TACCTGAACT CAGCAGTGAC ATAGACAGCA GCAATTTTGA TGACATTGAA 
GATGATAAAG GAGATGTAGA AACCTTCCCA ATTCCCAAGG CTTTTGTGGG AAATCAGCTA 
CCTTTTATAG GATTTACCTA CTACAGAGAA AATTTGCTAC TAAGTGACTC TCCATCTTGT 
AAAGAAAATG ACTCAATTCA ATCAAGGAAG AATGAAGAGA GTCAAGAGAT TCAGAAAAAA 
CTGTACACAT TAGAAGAACA CCTTAGCACT GAGATTCAGG CCAAAGAGGA ACTAGAACAG 
AAGTGCAAGT CTGTTAATAC TCGCTTAGAG AAAGTGGCAA AGGAGTTAGA AGAAGAGATT 
ACCTTAAGGA AAAATGTGGA ATCAACATTA AGACAATTAG AAAGAGAAAA AGCACTTCTT 
CAGCACAAAA ATGCAGAATA TCAGCGGAAA GCTGATCATG AAGCAGACAA GAAGCGAAAT 
TTGGAGAATG ATGTTAACAG TTTAAAAGAT CAGCTTGAAG ATTTGAAAAA AAGAAATCAG 
AACTCTCAGA TATCCACTGA GAAAGTGAAT CAACTCCAGA GACAACTGGA TGAAACCAAT 
GCTTTGCTGC GAACAGAATC TGATACTGCA GCCCGGTTAA GGAAAACACA GGCAGAAAGT 
TCAAAACAGA TTCAGCAGCT GGAATCTAAC AATAGAGATC TACAAGACAA AAATTGCCTG 
CTGGAGACTG CCAAGTTAAA ACTTGAAAAG GAATTTATCA ATCTTCAGTC AGTTCTAGAA 
TCTGAAAGGA GGGACCGAAC CCATGGATCA GAGATTATTA ATGATTTACA AGGTAGAATA 
TCTGGCCTAG AAGAAGATGT AAAGAATGGT AAAATCTTAT TAGCAAAAGT AGAGCTGGAG 
AAGAGACAAC TACAGGAGAG ATTTACTGAT TTGGAAAAGG AAAAGAACAA CATGGAAATA 
GATATGACAT ACCAACTAAA AGTCATACAG CAAAGTTTAG AACAAGAAGA AACTGAACAT 
AAGGCTACAA AAGCACGGCT TGCAGATAAA AACAAGATTT ATGAATCCAT TGAAGAAGCT 
AAATCAGAAG CCATGAAAGA AATGGAGAAA AAGCTCTCGG AGGAAAGAAC TTTAAAACAG 
AAAGTAGAGA ACTTGTTGCT GGAGGCTGAG AAAAGATGCT CTATATTAGA CTGTGACCTC 
AAACAGTCAC AGCAGAAAAT AAATGAACTC CTCAAACAGA AAGATGTGCT AAATGAGGAT 
GTTAGAAACT TGACATTAAA AATAGAACAG GAAACTCAGA AGCGCTGCCT TACACAAAAT 
GACTTGAAGA TGCAAACACA GCAAGTTAAC ACACTAAAAA TGTCAGAAAA GCAGTTAAAG 
CAAGAGAATA ATCATCTCCT AGAAATGAAA ATGAGCTTGG AAAAACAGAA TGCTGAACTT 
CGAAAAGAAC GTCAAGATGC AGATGGACAG ATGAAAGAGC TCCAGGATCA GCTTGAAGCA 
GAGCAGTATT TCTCAACCCT CTATAAAACA CAGGTTAGGG AACTTAAGGA AGAATGTGAA 
GAAAAGACCA AACTTTGTAA AGAATTACAG CAGAAGAAGC AGGAATTACA GGATGAAAGG 
GACTCCTTGG CTGCTCAACT GGAGATTACC TTAACCAAAG CAGATTCTGA GCAACTGGCT 
CGTTCAATTG CTGAGGAACA GTATTCTGAT TTGGAAAAAG AGAAGATCAT GAAAGAGCTG 
GAGATCAAAG AGATGATGGC TCGACACAAA CAGGAACTCA CCGAAAAAGA TGCTACTATT 
GCGTCTCTTG AAGAAACTAA TAGGACACTA ACTAGTGATG TTGCCAATCT TGCAAATGAG 
AAAGAAGAAT TAAATAACAA ACTGAAGGAA GCCCAAGAGC AACTATCAAG GTTGAAAGAT 
GAAGAAATAA GTGCAGCAGC TATTAAAGCA CAATTTGAGA AGCAGCTGTT AACAGAGAGG 
ACACTCAAAA CTCAAGCTGT GAATAAGTTG GCTGAGATCA TGAATCGAAA GGAACCTGTT 
AAGCGTGGTA ATGACACAGA TGTGCGGAGA AAAGAAAAGG AGAATAGAAA GCTACATATG 
GAACTTAAAT CTGAACGCGA AAAACTGACC CAGCAGATGA TCAAGTATCA GAAAGAACTG 
AATGAAATGC AGGCTCAAAT AGCTGAAGAG AGTCAGATTC GAATTGAACT ACAGATGACA 
CTGGACAGTA AGGACAGTGA CATTGAGCAG CTGCGCTCCC AGCTCCAGGC CTTGCACATT 
GGTTTGGATA GTTCCAGTAT AGGCAGTGGA CCAGGGGATA CTGAAGCTGA TGACGGTTTT 
CCAGTTCATA TCACTCAGTC ACACACTATG GAATCCATGT CATTCACCTA CCAACGTTCC 
TCCACTTCTC TCAATATTGC CACTAAGCCT TCCAGTTCTC ATATGCTTCT TGACTCTGAT 
TCTGACTCAG AAGAAGAATC TGTTCCTTAC TTACCTATTT CATCGGAACC TAATGAATCA 
AGACTAGAAG GATGGCTTTC ATTGCCTGTG CGAAACAACA CTAAGAAATT TGGATGGGTT 
AAAAAGTATG TGATTGTAAG CAGTAAGAAG ATCCTTTTCT ATGACAGTGA GCAAGATAAA 
GAACAATCTA ATCCTTACAT GGTTTTAGAT ATAGACAAGT TATTTCATGT CCGACCAGTT 
ACACAGACAG ATGTATATAG AGCAGATGCT AAAGAAATTC CAAGGATATT CCAGATTCTG 
TATGCCAACG AAGGAGAAAG TAAGAAGGAA CAAGAATTTC CAGTGGAGCC AGTGGGAGAA 
AAATCAAATT ATATTTGCCA CAAGGGACAT GAATTTATTC CTACTCTGTA TCATTTCCCA 
ACCAACTGTG AGGCATGTAT GAAGCCATTG TGGCACATGT TTAAACCCCC TCCTGCTTTA 
GAGTGCCGTC GCTGTCATAT TAAATGTCAT AAAGATCACA TGGACAAAAA GGAGGAAATT 
ATAGCGCCTT GCAAAGTGTA TTATGATATT TCATCGGCAA AGAATCTATT GTTATTGGCA 
AATTCTACAG AAGAGCAGCA AAAGTGGGTT AGTCGGTTAG TGAAAAAAAT ACCTAAAAAG 
CCTCCAGCTC CAGACCCTTT TGCACGGTCA TCTCCTAGAA CGTCAATGAA AATACAACAA 
AACCAGTCTA TTCGACGGCC AAGTCGACAA CTTGCTCCAA ACAAACCAAG ATTGCTTGAT 
TTGGCATTTA AAGACTGGGA TTGGTCATTT GATGATGTTG ATGGTGGTGA TGAGGATGAC 
GATGTTTTTG ATTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROCK2 ( NM_174452.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776877.1 Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), mRNA.

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CloneID	OBa43936
Clone ID Related Accession (Same CDS sequence)	NM_174452.2
Accession Version	NM_174452.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	rho-associated protein kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U36909.1. On or before Mar 29, 2005 this sequence version replaced XM_581335.1, NM_174452.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U36909.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATCCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGAA TCAAGACTAG AAGGATGGCT TTCATTGCCT 
GTGCGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATCCTTT TCTATGACAG TGAGCAAGAT AAAGAACAAT CTAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTATA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ACGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTGGGA GAAAAATCAA ATTATATTTG CCACAAGGGA 
CATGAATTTA TTCCTACTCT GTATCATTTC CCAACCAACT GTGAGGCATG TATGAAGCCA 
TTGTGGCACA TGTTTAAACC CCCTCCTGCT TTAGAGTGCC GTCGCTGTCA TATTAAATGT 
CATAAAGATC ACATGGACAA AAAGGAGGAA ATTATAGCGC CTTGCAAAGT GTATTATGAT 
ATTTCATCGG CAAAGAATCT ATTGTTATTG GCAAATTCTA CAGAAGAGCA GCAAAAGTGG 
GTTAGTCGGT TAGTGAAAAA AATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCACGG 
TCATCTCCTA GAACGTCAAT GAAAATACAA CAAAACCAGT CTATTCGACG GCCAAGTCGA 
CAACTTGCTC CAAACAAACC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776877.1
CDS	1..4167
Translation	MSRPPPTGKMPGAPEAVSGDGAGASRQRKLEALIRDPRSPINVESLLDGLNPLVLDLDFPALRKNKNIDNFLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFCAFQDDKYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGLIHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDAEISKHAKNLICAFLTDREVRLGRNGVEEIKQHPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYYRENLLLSDSPSCKENDSIQSRKNEESQEIQKKLYTLEEHLSTEIQAKEELEQKCKSVNTRLEKVAKELEEEITLRKNVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQNSQISTEKVNQLQRQLDETNALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSVLESERRDRTHGSEIINDLQGRISGLEEDVKNGKILLAKVELEKRQLQERFTDLEKEKNNMEIDMTYQLKVIQQSLEQEETEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLSEERTLKQKVENLLLEAEKRCSILDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLLEMKMSLEKQNAELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEECEEKTKLCKELQQKKQELQDERDSLAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDATIASLEETNRTLTSDVANLANEKEELNNKLKEAQEQLSRLKDEEISAAAIKAQFEKQLLTERTLKTQAVNKLAEIMNRKEPVKRGNDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLRSQLQALHIGLDSSSIGSGPGDTEADDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQDKEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEQEFPVEPVGEKSNYICHKGHEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLLANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS

Target ORF information:

RefSeq Version	NM_174452.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho associated coiled-coil containing protein kinase 2 (ROCK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174452.2

ORF Insert Sequence:

ATGAGCCGGC CCCCGCCGAC GGGGAAGATG CCCGGCGCCC CCGAGGCCGT GTCGGGGGAC 
GGCGCGGGCG CGAGCCGCCA GAGGAAGCTG GAAGCGCTGA TCCGAGACCC TCGTTCGCCC 
ATCAACGTGG AGAGCTTGCT GGATGGCTTA AATCCTTTGG TCCTTGATTT GGATTTTCCT 
GCTTTGAGGA AAAACAAAAA TATAGATAAT TTCTTAAATA GATATGAGAA AATTGTGAAA 
AAAATTAGAG GTTTACAGAT GAAGGCAGAA GACTACGATG TTGTAAAAGT TATCGGAAGA 
GGTGCTTTTG GTGAAGTCCA GTTGGTTCGT CATAAGGCAT CACAGAAAGT TTATGCTATG 
AAGCTTCTTA GTAAGTTTGA AATGATAAAA AGATCAGATT CTGCTTTTTT CTGGGAGGAA 
AGAGATATTA TGGCCTTTGC CAACAGTCCC TGGGTGGTTC AGCTCTTTTG TGCCTTTCAA 
GATGATAAGT ATCTGTACAT GGTAATGGAG TACATGCCTG GTGGAGACCT TGTAAACCTT 
ATGAGTAACT ATGATGTACC TGAAAAATGG GCCAAATTTT ATACTGCTGA AGTTGTTCTT 
GCTTTGGATG CCATACACTC CATGGGTTTA ATTCACAGAG ATGTGAAGCC TGACAACATG 
CTCTTGGATA AACATGGGCA TCTAAAATTA GCAGATTTTG GCACATGTAT GAAGATGGAT 
GAAACAGGCA TGGTGCATTG TGATACAGCA GTTGGAACAC CCGATTATAT ATCACCCGAG 
GTCCTGAAAT CACAAGGGGG TGATGGTTAC TATGGGCGAG AATGTGATTG GTGGTCCGTG 
GGTGTTTTCC TTTTTGAAAT GCTGGTGGGG GATACTCCAT TTTATGCAGA TTCACTTGTA 
GGAACATATA GCAAAATTAT GGATCATAAA AACTCACTAT GTTTCCCTGA AGATGCAGAA 
ATTTCTAAAC ATGCGAAGAA TCTCATCTGT GCCTTCTTAA CAGATAGGGA GGTACGCCTT 
GGAAGAAACG GGGTAGAAGA AATCAAACAA CATCCTTTCT TTAAGAATGA TCAGTGGAAT 
TGGGATAACA TAAGAGAGAC TGCAGCTCCT GTGGTACCTG AACTCAGCAG TGACATAGAC 
AGCAGCAATT TTGATGACAT TGAAGATGAT AAAGGAGATG TAGAAACCTT CCCAATTCCC 
AAGGCTTTTG TGGGAAATCA GCTACCTTTT ATAGGATTTA CCTACTACAG AGAAAATTTG 
CTACTAAGTG ACTCTCCATC TTGTAAAGAA AATGACTCAA TTCAATCAAG GAAGAATGAA 
GAGAGTCAAG AGATTCAGAA AAAACTGTAC ACATTAGAAG AACACCTTAG CACTGAGATT 
CAGGCCAAAG AGGAACTAGA ACAGAAGTGC AAGTCTGTTA ATACTCGCTT AGAGAAAGTG 
GCAAAGGAGT TAGAAGAAGA GATTACCTTA AGGAAAAATG TGGAATCAAC ATTAAGACAA 
TTAGAAAGAG AAAAAGCACT TCTTCAGCAC AAAAATGCAG AATATCAGCG GAAAGCTGAT 
CATGAAGCAG ACAAGAAGCG AAATTTGGAG AATGATGTTA ACAGTTTAAA AGATCAGCTT 
GAAGATTTGA AAAAAAGAAA TCAGAACTCT CAGATATCCA CTGAGAAAGT GAATCAACTC 
CAGAGACAAC TGGATGAAAC CAATGCTTTG CTGCGAACAG AATCTGATAC TGCAGCCCGG 
TTAAGGAAAA CACAGGCAGA AAGTTCAAAA CAGATTCAGC AGCTGGAATC TAACAATAGA 
GATCTACAAG ACAAAAATTG CCTGCTGGAG ACTGCCAAGT TAAAACTTGA AAAGGAATTT 
ATCAATCTTC AGTCAGTTCT AGAATCTGAA AGGAGGGACC GAACCCATGG ATCAGAGATT 
ATTAATGATT TACAAGGTAG AATATCTGGC CTAGAAGAAG ATGTAAAGAA TGGTAAAATC 
TTATTAGCAA AAGTAGAGCT GGAGAAGAGA CAACTACAGG AGAGATTTAC TGATTTGGAA 
AAGGAAAAGA ACAACATGGA AATAGATATG ACATACCAAC TAAAAGTCAT ACAGCAAAGT 
TTAGAACAAG AAGAAACTGA ACATAAGGCT ACAAAAGCAC GGCTTGCAGA TAAAAACAAG 
ATTTATGAAT CCATTGAAGA AGCTAAATCA GAAGCCATGA AAGAAATGGA GAAAAAGCTC 
TCGGAGGAAA GAACTTTAAA ACAGAAAGTA GAGAACTTGT TGCTGGAGGC TGAGAAAAGA 
TGCTCTATAT TAGACTGTGA CCTCAAACAG TCACAGCAGA AAATAAATGA ACTCCTCAAA 
CAGAAAGATG TGCTAAATGA GGATGTTAGA AACTTGACAT TAAAAATAGA ACAGGAAACT 
CAGAAGCGCT GCCTTACACA AAATGACTTG AAGATGCAAA CACAGCAAGT TAACACACTA 
AAAATGTCAG AAAAGCAGTT AAAGCAAGAG AATAATCATC TCCTAGAAAT GAAAATGAGC 
TTGGAAAAAC AGAATGCTGA ACTTCGAAAA GAACGTCAAG ATGCAGATGG ACAGATGAAA 
GAGCTCCAGG ATCAGCTTGA AGCAGAGCAG TATTTCTCAA CCCTCTATAA AACACAGGTT 
AGGGAACTTA AGGAAGAATG TGAAGAAAAG ACCAAACTTT GTAAAGAATT ACAGCAGAAG 
AAGCAGGAAT TACAGGATGA AAGGGACTCC TTGGCTGCTC AACTGGAGAT TACCTTAACC 
AAAGCAGATT CTGAGCAACT GGCTCGTTCA ATTGCTGAGG AACAGTATTC TGATTTGGAA 
AAAGAGAAGA TCATGAAAGA GCTGGAGATC AAAGAGATGA TGGCTCGACA CAAACAGGAA 
CTCACCGAAA AAGATGCTAC TATTGCGTCT CTTGAAGAAA CTAATAGGAC ACTAACTAGT 
GATGTTGCCA ATCTTGCAAA TGAGAAAGAA GAATTAAATA ACAAACTGAA GGAAGCCCAA 
GAGCAACTAT CAAGGTTGAA AGATGAAGAA ATAAGTGCAG CAGCTATTAA AGCACAATTT 
GAGAAGCAGC TGTTAACAGA GAGGACACTC AAAACTCAAG CTGTGAATAA GTTGGCTGAG 
ATCATGAATC GAAAGGAACC TGTTAAGCGT GGTAATGACA CAGATGTGCG GAGAAAAGAA 
AAGGAGAATA GAAAGCTACA TATGGAACTT AAATCTGAAC GCGAAAAACT GACCCAGCAG 
ATGATCAAGT ATCAGAAAGA ACTGAATGAA ATGCAGGCTC AAATAGCTGA AGAGAGTCAG 
ATTCGAATTG AACTACAGAT GACACTGGAC AGTAAGGACA GTGACATTGA GCAGCTGCGC 
TCCCAGCTCC AGGCCTTGCA CATTGGTTTG GATAGTTCCA GTATAGGCAG TGGACCAGGG 
GATACTGAAG CTGATGACGG TTTTCCAGAA TCAAGACTAG AAGGATGGCT TTCATTGCCT 
GTGCGAAACA ACACTAAGAA ATTTGGATGG GTTAAAAAGT ATGTGATTGT AAGCAGTAAG 
AAGATCCTTT TCTATGACAG TGAGCAAGAT AAAGAACAAT CTAATCCTTA CATGGTTTTA 
GATATAGACA AGTTATTTCA TGTCCGACCA GTTACACAGA CAGATGTATA TAGAGCAGAT 
GCTAAAGAAA TTCCAAGGAT ATTCCAGATT CTGTATGCCA ACGAAGGAGA AAGTAAGAAG 
GAACAAGAAT TTCCAGTGGA GCCAGTGGGA GAAAAATCAA ATTATATTTG CCACAAGGGA 
CATGAATTTA TTCCTACTCT GTATCATTTC CCAACCAACT GTGAGGCATG TATGAAGCCA 
TTGTGGCACA TGTTTAAACC CCCTCCTGCT TTAGAGTGCC GTCGCTGTCA TATTAAATGT 
CATAAAGATC ACATGGACAA AAAGGAGGAA ATTATAGCGC CTTGCAAAGT GTATTATGAT 
ATTTCATCGG CAAAGAATCT ATTGTTATTG GCAAATTCTA CAGAAGAGCA GCAAAAGTGG 
GTTAGTCGGT TAGTGAAAAA AATACCTAAA AAGCCTCCAG CTCCAGACCC TTTTGCACGG 
TCATCTCCTA GAACGTCAAT GAAAATACAA CAAAACCAGT CTATTCGACG GCCAAGTCGA 
CAACTTGCTC CAAACAAACC AAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Interaction between ROCK II and nucleophosmin/B23 in the regulation of centrosome duplication.
Molecular and cellular biology26(23)9016-34(2006 Dec)
Ma Z,Kanai M,Kawamura K,Kaibuchi K,Ye K,Fukasawa K

Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase.
The Journal of biological chemistry281(22)15320-9(2006 Jun)
Tanaka T,Nishimura D,Wu RC,Amano M,Iso T,Kedes L,Nishida H,Kaibuchi K,Hamamori Y

RhoA and Rho-kinase dependent and independent signals mediate TGF-beta-induced pulmonary endothelial cytoskeletal reorganization and permeability.
American journal of physiology. Lung cellular and molecular physiology288(2)L294-306(2005 Feb)
Clements RT,Minnear FL,Singer HA,Keller RS,Vincent PA

Rho kinase-induced nuclear translocation of ERK1/ERK2 in smooth muscle cell mitogenesis caused by serotonin.
Circulation research95(6)579-86(2004 Sep)
Liu Y,Suzuki YJ,Day RM,Fanburg BL

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ROCK2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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