PDE6A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE6A
Entrez Gene ID	281973
Full Name	phosphodiesterase 6A
General protein information	Preferred Names phosphodiesterase 6A Names rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha GMP-PDE alpha PDE V-B1 phosphodiesterase 6A, cGMP-specific, rod, alpha phosphodiesterase, cyclic GMP (rod receptor), alpha polypeptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010807419.3	XP_010805721.2	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
XM_010807419.2	XP_010805721.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
XM_024993841.1	XP_024849609.1	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X2
NM_001001526.2	NP_001001526.2	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE6A	NP_000431.2
Pan troglodytes (chimpanzee)	PDE6A	XP_518030.2
Mus musculus (house mouse)	Pde6a	NP_666198.1
Rattus norvegicus (Norway rat)	Pde6a	NP_001100856.1
Xenopus tropicalis (tropical clawed frog)	pde6a	XP_002936750.2
Canis lupus familiaris (dog)	PDE6A	NP_001003073.1
Danio rerio (zebrafish)	pde6a	NP_001007161.1
Bos taurus (cattle)	PDE6A	NP_001001526.2
	PDE6A	XP_001107349.1

Gene Ontology
Bibliography

Related articles in PubMed

Domain organization and conformational plasticity of the G protein effector, PDE6.
Zhang Z, He F, Constantine R, Baker ML, Baehr W, Schmid MF, Wensel TG, Agosto MA
The Journal of biological chemistry290(20)12833-43(2015 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Direct allosteric regulation between the GAF domain and catalytic domain of photoreceptor phosphodiesterase PDE6.
Zhang XJ, Cahill KB, Elfenbein A, Arshavsky VY, Cote RH
The Journal of biological chemistry283(44)29699-705(2008 Oct)

Glutamic acid-rich proteins of rod photoreceptors are natively unfolded.
Batra-Safferling R, Abarca-Heidemann K, K?rschen HG, Tziatzios C, Stoldt M, Budyak I, Willbold D, Schwalbe H, Klein-Seetharaman J, Kaupp UB
The Journal of biological chemistry281(3)1449-60(2006 Jan)

Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.
Guo LW, Grant JE, Hajipour AR, Muradov H, Arbabian M, Artemyev NO, Ruoho AE
The Journal of biological chemistry280(13)12585-92(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The approximately 11 A map revealed previously unseen features of PDE6, and each domain was readily fit with high resolution structures.
Title: Domain organization and conformational plasticity of the G protein effector, PDE6.

although Pgamma-mediated regulation plays the dominant role in visual excitation, the direct, inter-domain allosteric regulation may play a feedback role in light adaptational processes during phototransduction
Title: Direct allosteric regulation between the GAF domain and catalytic domain of photoreceptor phosphodiesterase PDE6.

the unique Palphabeta heterodimer contributes to the sophisticated regulation in visual transduction through interaction with Pgamma
Title: Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.

Structural analysis of cGMP phosphodiesterase 6 alpha, beta and gamma interactions
Title: Three-dimensional structure of non-activated cGMP phosphodiesterase 6 and comparison of its image with those of activated forms.

The following PDE6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41306	NM_001001526.2 Latest version!	Bos taurus phosphodiesterase 6A (PDE6A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa45299	XM_010807419.2 Latest version!	Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa235765	XM_010807419.3 Latest version!	Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa235766	XM_024993841.1 Latest version!	Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE6A ( NM_001001526.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001001526.2 Bos taurus phosphodiesterase 6A (PDE6A), mRNA.

Price & Availability↑

CloneID	OBa41306
Clone ID Related Accession (Same CDS sequence)	NM_001001526.2
Accession Version	NM_001001526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Bos taurus(cattle)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X12756.1. On or before Mar 29, 2005 this sequence version replaced XM_607066.1, NM_001001526.1. ##Evidence-Data-START## Transcript exon combination :: M27541.1, M36683.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGGGCGAGG TGACGGCAGA GGAAGTAGAG AAGTTTCTGG ACTCAAATGT CAGCTTTGCC 
AAACAGTACT ACAACCTGCG CTACCGGGCC AAGGTCATCT CAGACCTGCT GGGACCCAGG 
GAGGCGGCCG TGGACTTCAG CAACTACCAT GCGCTGAACA GCGTGGAAGA GAGTGAAATT 
ATCTTCGACC TCCTGCGGGA CTTTCAGGAC AACCTGCAGG CCGAGAAGTG CGTCTTCAAT 
GTCATGAAGA AGTTGTGCTT CCTGCTGCAG GCCGACCGCA TGAGCCTATT CATGTACAGG 
GCCCGGAACG GCATCGCAGA GCTGGCCACC CGGCTCTTCA ACGTCCACAA GGATGCTGTG 
CTCGAGGAGT GCCTGGTGGC GCCTGACTCG GAGATCGTGT TCCCCCTGGA CATGGGAGTG 
GTGGGCCATG TTGCGCTCTC TAAAAAGATC GTCAACGTCC CCAACACAGA GGAGGATGAA 
CATTTCTGTG ACTTTGTGGA CACCCTCACA GAGTACCAGA CTAAGAACAT CTTGGCTTCT 
CCCATAATGA ATGGGAAGGA TGTGGTGGCC ATAATCATGG CTGTGAATAA AGTGGATGGG 
CCCCACTTCA CCGAGAATGA TGAAGAGATT CTTCTCAAGT ACCTCAATTT TGCAAATCTA 
ATCATGAAGG TGTTCCACCT GAGTTACCTG CACAACTGTG AGACTCGGCG TGGCCAGATA 
CTGTTGTGGT CTGGGAGCAA AGTCTTTGAA GAGCTTACAG ACATTGAGAG GCAGTTCCAC 
AAAGCCCTGT ACACAGTCCG CGCCTTCCTC AACTGTGACA GATACTCTGT GGGGCTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TCCTGATGGG GGAGGCTCCA 
CCCTACGCTG GTCCCAGGAC TCCGGATGGA AGGGAAATCA ACTTTTACAA GGTCATTGAC 
TACATCCTGC ATGGCAAAGA AGACATCAAA GTCATCCCGA ATCCTCCTCC AGACCACTGG 
GCTTTAGTGA GCGGTCTCCC CACTTATGTT GCCCAAAATG GCCTGATTTG CAACATCATG 
AACGCGCCTT CAGAGGACTT TTTTGCATTC CAGAAAGAGC CTCTGGATGA GTCTGGATGG 
ATGATTAAAA ATGTCCTTTC TATGCCAATT GTGAACAAGA AGGAGGAAAT TGTTGGGGTG 
GCCACGTTTT ACAATCGCAA AGATGGAAAA CCCTTTGATG AAATGGATGA GACCCTCATG 
GAGTCTTTGG CCCAGTTCCT GGGCTGGTCT GTCTTAAATC CTGACACTTA TGAGTTGATG 
AACAAACTTG AAAACAGGAA GGATATTTTC CAAGACATGG TGAAATACCA CGTGAAGTGT 
GACAATGAAG AAATCCAGAC AATCTTGAAA ACCAGAGAGG TGTATGGGAA GGAGCCGTGG 
GAATGCGAGG AAGAAGAACT GGCTGAGATC CTGCAAGGAG AGCTGCCAGA TGCAGACAAA 
TATGAAATTA ATAAATTCCA CTTCAGCGAC CTGCCCCTGA CGGAACTGGA GCTGGTGAAA 
TGTGGAATTC AGATGTACTA TGAGCTCAAA GTGGTGGATA AATTTCACAT TCCTCAGGAG 
GCCCTGGTGC GGTTCATGTA TTCCCTGAGC AAGGGCTACC GCAGGATCAC CTACCACAAC 
TGGCGGCACG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC CGGAAAGCTG 
AAGCGATACT TCACAGACCT GGAGGCCTTG GCCATGGTCA CCGCCGCCTT CTGCCATGAC 
ATTGACCACA GAGGCACTAA CAATCTCTAC CAGATGAAAT CCCAGAACCC ACTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGACAC CACTTGGAGT TTGGTAAAAC ACTGTTGAGA 
GATGAGAGCC TAAATATCTT TCAGAACCTC AATCGCAGGC AGCACGAGCA TGCAATCCAC 
ATGATGGACA TCGCGATCAT TGCTACAGAC CTTGCCTTGT ATTGCAAGAA AAGGACGATG 
TTCCAAAAGA TCGTGGATCA GTCTAAGACA TACGAGACTC AGCAGGAGTG GACACAGTAC 
ATGATGCTGG ATCAGACACG GAAGGAAATT GTCATGGCCA TGATGATGAC CGCCTGTGAT 
CTCTCAGCCA TCACCAAGCC TTGGGAGGTG CAGAGCAAGG TGGCTCTGCT GGTTGCTGCT 
GAATTCTGGG AACAAGGTGA CCTGGAGCGC ACGGTGCTGC AACAGAATCC CATTCCCATG 
ATGGACAGAA ACAAGGCGGA TGAACTCCCT AAGCTTCAGG TCGGCTTCAT CGACTTTGTT 
TGCACCTTTG TCTACAAGGA ATTCTCCCGT TTCCACGAGG AGATCACTCC CATGCTAGAT 
GGGATTACCA ACAACCGCAA GGAGTGGAAA GCGCTTGCTG ATGAGTATGA GACCAAGATG 
AAGGGGCTGG AGGAGGAGAA GCAGAAACAG CAGGCAGCCA ACCAAGCAGC CGCAGGAAGT 
CAACACGGCG GAAAGCAACC TGGGGGCGGG CCTGCATCCA AGTCTTGCTG CGTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001526.2
CDS	91..2670
Translation	MGEVTAEEVEKFLDSNVSFAKQYYNLRYRAKVISDLLGPREAAVDFSNYHALNSVEESEIIFDLLRDFQDNLQAEKCVFNVMKKLCFLLQADRMSLFMYRARNGIAELATRLFNVHKDAVLEECLVAPDSEIVFPLDMGVVGHVALSKKIVNVPNTEEDEHFCDFVDTLTEYQTKNILASPIMNGKDVVAIIMAVNKVDGPHFTENDEEILLKYLNFANLIMKVFHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEAPPYAGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPPDHWALVSGLPTYVAQNGLICNIMNAPSEDFFAFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEMDETLMESLAQFLGWSVLNPDTYELMNKLENRKDIFQDMVKYHVKCDNEEIQTILKTREVYGKEPWECEEEELAEILQGELPDADKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYCKKRTMFQKIVDQSKTYETQQEWTQYMMLDQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYETKMKGLEEEKQKQQAANQAAAGSQHGGKQPGGGPASKSCCVQ

Target ORF information:

RefSeq Version	NM_001001526.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6A (PDE6A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001526.2

ORF Insert Sequence:

ATGGGCGAGG TGACGGCAGA GGAAGTAGAG AAGTTTCTGG ACTCAAATGT CAGCTTTGCC 
AAACAGTACT ACAACCTGCG CTACCGGGCC AAGGTCATCT CAGACCTGCT GGGACCCAGG 
GAGGCGGCCG TGGACTTCAG CAACTACCAT GCGCTGAACA GCGTGGAAGA GAGTGAAATT 
ATCTTCGACC TCCTGCGGGA CTTTCAGGAC AACCTGCAGG CCGAGAAGTG CGTCTTCAAT 
GTCATGAAGA AGTTGTGCTT CCTGCTGCAG GCCGACCGCA TGAGCCTATT CATGTACAGG 
GCCCGGAACG GCATCGCAGA GCTGGCCACC CGGCTCTTCA ACGTCCACAA GGATGCTGTG 
CTCGAGGAGT GCCTGGTGGC GCCTGACTCG GAGATCGTGT TCCCCCTGGA CATGGGAGTG 
GTGGGCCATG TTGCGCTCTC TAAAAAGATC GTCAACGTCC CCAACACAGA GGAGGATGAA 
CATTTCTGTG ACTTTGTGGA CACCCTCACA GAGTACCAGA CTAAGAACAT CTTGGCTTCT 
CCCATAATGA ATGGGAAGGA TGTGGTGGCC ATAATCATGG CTGTGAATAA AGTGGATGGG 
CCCCACTTCA CCGAGAATGA TGAAGAGATT CTTCTCAAGT ACCTCAATTT TGCAAATCTA 
ATCATGAAGG TGTTCCACCT GAGTTACCTG CACAACTGTG AGACTCGGCG TGGCCAGATA 
CTGTTGTGGT CTGGGAGCAA AGTCTTTGAA GAGCTTACAG ACATTGAGAG GCAGTTCCAC 
AAAGCCCTGT ACACAGTCCG CGCCTTCCTC AACTGTGACA GATACTCTGT GGGGCTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TCCTGATGGG GGAGGCTCCA 
CCCTACGCTG GTCCCAGGAC TCCGGATGGA AGGGAAATCA ACTTTTACAA GGTCATTGAC 
TACATCCTGC ATGGCAAAGA AGACATCAAA GTCATCCCGA ATCCTCCTCC AGACCACTGG 
GCTTTAGTGA GCGGTCTCCC CACTTATGTT GCCCAAAATG GCCTGATTTG CAACATCATG 
AACGCGCCTT CAGAGGACTT TTTTGCATTC CAGAAAGAGC CTCTGGATGA GTCTGGATGG 
ATGATTAAAA ATGTCCTTTC TATGCCAATT GTGAACAAGA AGGAGGAAAT TGTTGGGGTG 
GCCACGTTTT ACAATCGCAA AGATGGAAAA CCCTTTGATG AAATGGATGA GACCCTCATG 
GAGTCTTTGG CCCAGTTCCT GGGCTGGTCT GTCTTAAATC CTGACACTTA TGAGTTGATG 
AACAAACTTG AAAACAGGAA GGATATTTTC CAAGACATGG TGAAATACCA CGTGAAGTGT 
GACAATGAAG AAATCCAGAC AATCTTGAAA ACCAGAGAGG TGTATGGGAA GGAGCCGTGG 
GAATGCGAGG AAGAAGAACT GGCTGAGATC CTGCAAGGAG AGCTGCCAGA TGCAGACAAA 
TATGAAATTA ATAAATTCCA CTTCAGCGAC CTGCCCCTGA CGGAACTGGA GCTGGTGAAA 
TGTGGAATTC AGATGTACTA TGAGCTCAAA GTGGTGGATA AATTTCACAT TCCTCAGGAG 
GCCCTGGTGC GGTTCATGTA TTCCCTGAGC AAGGGCTACC GCAGGATCAC CTACCACAAC 
TGGCGGCACG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC CGGAAAGCTG 
AAGCGATACT TCACAGACCT GGAGGCCTTG GCCATGGTCA CCGCCGCCTT CTGCCATGAC 
ATTGACCACA GAGGCACTAA CAATCTCTAC CAGATGAAAT CCCAGAACCC ACTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGACAC CACTTGGAGT TTGGTAAAAC ACTGTTGAGA 
GATGAGAGCC TAAATATCTT TCAGAACCTC AATCGCAGGC AGCACGAGCA TGCAATCCAC 
ATGATGGACA TCGCGATCAT TGCTACAGAC CTTGCCTTGT ATTGCAAGAA AAGGACGATG 
TTCCAAAAGA TCGTGGATCA GTCTAAGACA TACGAGACTC AGCAGGAGTG GACACAGTAC 
ATGATGCTGG ATCAGACACG GAAGGAAATT GTCATGGCCA TGATGATGAC CGCCTGTGAT 
CTCTCAGCCA TCACCAAGCC TTGGGAGGTG CAGAGCAAGG TGGCTCTGCT GGTTGCTGCT 
GAATTCTGGG AACAAGGTGA CCTGGAGCGC ACGGTGCTGC AACAGAATCC CATTCCCATG 
ATGGACAGAA ACAAGGCGGA TGAACTCCCT AAGCTTCAGG TCGGCTTCAT CGACTTTGTT 
TGCACCTTTG TCTACAAGGA ATTCTCCCGT TTCCACGAGG AGATCACTCC CATGCTAGAT 
GGGATTACCA ACAACCGCAA GGAGTGGAAA GCGCTTGCTG ATGAGTATGA GACCAAGATG 
AAGGGGCTGG AGGAGGAGAA GCAGAAACAG CAGGCAGCCA ACCAAGCAGC CGCAGGAAGT 
CAACACGGCG GAAAGCAACC TGGGGGCGGG CCTGCATCCA AGTCTTGCTG CGTCCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6A ( XM_010807419.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805721.1 Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45299
Clone ID Related Accession (Same CDS sequence)	XM_010807419.2
Accession Version	XM_010807419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104039.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG TGACGGCAGA GGAAGTAGAG AAGTTTCTGG ACTCAAATGT CAGCTTTGCC 
AAACAGTACT ACAACCTGCG CTACCGGGCC AAGGTCATCT CAGACCTGCT GGGACCCAGG 
GAGGCGGCCG TGGACTTCAG CAACTACCAT GCGCTGAACA GCGTGGAAGA GAGTGAAATT 
ATCTTCGACC TCCTGCGGGA CTTTCAGGAC AACCTGCAGG CCGAGAAGTG CGTCTTCAAT 
GTCATGAAGA AGTTGTGCTT CCTGCTGCAG GCCGACCGCA TGAGCCTATT CATGTACAGG 
GCCCGGAACG GCATCGCAGA GCTGGCCACC CGGCTCTTCA ACGTCCACAA GGATGCTGTG 
CTCGAGGAGT GCCTGGTGGC GCCTGACTCG GAGATCGTGT TCCCCCTGGA CATGGGAGTG 
GTGGGCCATG TTGCGCTCTC TAAAAAGATC GTCAACGTCC CCAACACAGA GGAGGATGAA 
CATTTCTGTG ACTTTGTGGA CACCCTCACA GAGTACCAGA CTAAGAACAT CTTGGCTTCT 
CCCATAATGA ATGGGAAGGA TGTGGTGGCC ATAATCATGG TTGTGAATAA AGTGGATGGG 
CCCCACTTCA CCGAGAATGA TGAAGAGATT CTTCTCAAGT ACCTCAATTT TGCAAATCTA 
ATCATGAAGG TGTTCCACCT GAGTTACCTG CACAACTGTG AGACTCGGCG TGGCCAGATA 
CTGTTGTGGT CTGGGAGCAA AGTCTTTGAA GAGCTTACAG ACATTGAGAG GCAGTTCCAC 
AAAGCCCTGT ACACAGTCCG CGCCTTCCTC AACTGTGACA GATACTCTGT GGGGCTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TCCTGATGGG GGAGGCTCCA 
CCCTACGCTG GTCCCAGGAC TCCGGATGGA AGGGAAATCA ACTTTTACAA GGTCATTGAC 
TACATCCTGC ATGGCAAAGA AGACATCAAA GTCATCCCGA ATCCTCCTCC AGACCACTGG 
GCTTTAGTGA GCGGTCTCCC CACTTATGTT GCCCAAAATG GCCTGATTTG CAACATCATG 
AATGCGCCTT CAGAGGACTT TTTTGCATTC CAGAAAGAGC CTCTGGATGA GTCTGGATGG 
GTGATTAAAA ATGTCCTTTC TATGCCAATT GTGAACAAGA AGGAGGAAAT TGTTGGGGTG 
GCCACGTTTT ACAATCGCAA AGATGGAAAA CCCTTTGATG AAATGGATGA GACCCTCATG 
GAGTCTTTGG CCCAGTTCCT GGGCTGGTCT GTCTTAAATC CTGACACTTA TGAGTTGATG 
AACAAACTTG AAAACAGGAA GGATATTTTC CAAGACATGG TGAAATACCA CGTGAAGTGT 
GACAATGAAG AAATCCAGAC AATCTTGAAA ACCAGAGAGG TGTATGGGAA GGAGCCGTGG 
GAATGCGAGG AAGAAGAACT GGCTGAGATC CTGCAAGGAG AGCTGCCAGA TGCAGACAAA 
TATGAAATTA ATAAATTCCA CTTCAGCGAC CTGCCCCTGA CGGAACTGGA GCTGGTGAAA 
TGTGGAATTC AGATGTACTA TGAGCTCAAA GTGGTGGATA AATTTCACAT TCCTCAGGAG 
GCCCTGGTGC GGTTCATGTA TTCCCTGAGT AAGGGCTACC GCAGGATCAC CTACCACAAC 
TGGCGGCACG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC CGGAAAGCTG 
AAGCGATACT TCACAGACCT GGAGGCCTTG GCCATGGTCA CCGCCGCCTT CTGCCACGAC 
ATTGACCACA GAGGCACTAA CAATCTCTAC CAGATGAAAT CCCAGAACCC ACTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGACAC CACTTGGAGT TTGGTAAAAC ACTCTTGAGA 
GATGAGAGCC TAAATATCTT TCAGAACCTC AATCGCAGGC AGCATGAGCA TGCAATCCAC 
ATGATGGACA TTGCGATCAT TGCTACAGAC CTTGCCTTGT ATTTCAAGAA AAGGACGATG 
TTCCAAAAGA TCGTGGATCA GTCTAAGACA TACGAGACTC AGCAGGAGTG GACGCAGTAC 
ATGATGCTGG ATCAGACACG GAAGGAAATT GTCATGTGCT GCCAAGCAGC TCTGATGAGT 
CATGATCCGA CCCTGCAACG TCAATGCGCT CCCTGCTTAT TGGATACACC TGCACTCCCC 
GGCATGGGAT TGACACCCAC AGCACATGAC CCTGGATTCC CCTCCCAGAC TTCACGCCGC 
TGTGCCCCAT CTCTGAAATA CCTTATCAAA TTCTCTCCTC TTGCTCCAAT TCTTTTCACA 
TGTCCCTTCC CTTTCTGTCT TATGTCATAC CTACCTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805721.1
CDS	122..2503
Translation	MGEVTAEEVEKFLDSNVSFAKQYYNLRYRAKVISDLLGPREAAVDFSNYHALNSVEESEIIFDLLRDFQDNLQAEKCVFNVMKKLCFLLQADRMSLFMYRARNGIAELATRLFNVHKDAVLEECLVAPDSEIVFPLDMGVVGHVALSKKIVNVPNTEEDEHFCDFVDTLTEYQTKNILASPIMNGKDVVAIIMVVNKVDGPHFTENDEEILLKYLNFANLIMKVFHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEAPPYAGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPPDHWALVSGLPTYVAQNGLICNIMNAPSEDFFAFQKEPLDESGWVIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEMDETLMESLAQFLGWSVLNPDTYELMNKLENRKDIFQDMVKYHVKCDNEEIQTILKTREVYGKEPWECEEEELAEILQGELPDADKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYETQQEWTQYMMLDQTRKEIVMCCQAALMSHDPTLQRQCAPCLLDTPALPGMGLTPTAHDPGFPSQTSRRCAPSLKYLIKFSPLAPILFTCPFPFCLMSYLPV

Target ORF information:

RefSeq Version	XM_010807419.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807419.2

ORF Insert Sequence:

ATGGGCGAGG TGACGGCAGA GGAAGTAGAG AAGTTTCTGG ACTCAAATGT CAGCTTTGCC 
AAACAGTACT ACAACCTGCG CTACCGGGCC AAGGTCATCT CAGACCTGCT GGGACCCAGG 
GAGGCGGCCG TGGACTTCAG CAACTACCAT GCGCTGAACA GCGTGGAAGA GAGTGAAATT 
ATCTTCGACC TCCTGCGGGA CTTTCAGGAC AACCTGCAGG CCGAGAAGTG CGTCTTCAAT 
GTCATGAAGA AGTTGTGCTT CCTGCTGCAG GCCGACCGCA TGAGCCTATT CATGTACAGG 
GCCCGGAACG GCATCGCAGA GCTGGCCACC CGGCTCTTCA ACGTCCACAA GGATGCTGTG 
CTCGAGGAGT GCCTGGTGGC GCCTGACTCG GAGATCGTGT TCCCCCTGGA CATGGGAGTG 
GTGGGCCATG TTGCGCTCTC TAAAAAGATC GTCAACGTCC CCAACACAGA GGAGGATGAA 
CATTTCTGTG ACTTTGTGGA CACCCTCACA GAGTACCAGA CTAAGAACAT CTTGGCTTCT 
CCCATAATGA ATGGGAAGGA TGTGGTGGCC ATAATCATGG TTGTGAATAA AGTGGATGGG 
CCCCACTTCA CCGAGAATGA TGAAGAGATT CTTCTCAAGT ACCTCAATTT TGCAAATCTA 
ATCATGAAGG TGTTCCACCT GAGTTACCTG CACAACTGTG AGACTCGGCG TGGCCAGATA 
CTGTTGTGGT CTGGGAGCAA AGTCTTTGAA GAGCTTACAG ACATTGAGAG GCAGTTCCAC 
AAAGCCCTGT ACACAGTCCG CGCCTTCCTC AACTGTGACA GATACTCTGT GGGGCTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TCCTGATGGG GGAGGCTCCA 
CCCTACGCTG GTCCCAGGAC TCCGGATGGA AGGGAAATCA ACTTTTACAA GGTCATTGAC 
TACATCCTGC ATGGCAAAGA AGACATCAAA GTCATCCCGA ATCCTCCTCC AGACCACTGG 
GCTTTAGTGA GCGGTCTCCC CACTTATGTT GCCCAAAATG GCCTGATTTG CAACATCATG 
AATGCGCCTT CAGAGGACTT TTTTGCATTC CAGAAAGAGC CTCTGGATGA GTCTGGATGG 
GTGATTAAAA ATGTCCTTTC TATGCCAATT GTGAACAAGA AGGAGGAAAT TGTTGGGGTG 
GCCACGTTTT ACAATCGCAA AGATGGAAAA CCCTTTGATG AAATGGATGA GACCCTCATG 
GAGTCTTTGG CCCAGTTCCT GGGCTGGTCT GTCTTAAATC CTGACACTTA TGAGTTGATG 
AACAAACTTG AAAACAGGAA GGATATTTTC CAAGACATGG TGAAATACCA CGTGAAGTGT 
GACAATGAAG AAATCCAGAC AATCTTGAAA ACCAGAGAGG TGTATGGGAA GGAGCCGTGG 
GAATGCGAGG AAGAAGAACT GGCTGAGATC CTGCAAGGAG AGCTGCCAGA TGCAGACAAA 
TATGAAATTA ATAAATTCCA CTTCAGCGAC CTGCCCCTGA CGGAACTGGA GCTGGTGAAA 
TGTGGAATTC AGATGTACTA TGAGCTCAAA GTGGTGGATA AATTTCACAT TCCTCAGGAG 
GCCCTGGTGC GGTTCATGTA TTCCCTGAGT AAGGGCTACC GCAGGATCAC CTACCACAAC 
TGGCGGCACG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC CGGAAAGCTG 
AAGCGATACT TCACAGACCT GGAGGCCTTG GCCATGGTCA CCGCCGCCTT CTGCCACGAC 
ATTGACCACA GAGGCACTAA CAATCTCTAC CAGATGAAAT CCCAGAACCC ACTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGACAC CACTTGGAGT TTGGTAAAAC ACTCTTGAGA 
GATGAGAGCC TAAATATCTT TCAGAACCTC AATCGCAGGC AGCATGAGCA TGCAATCCAC 
ATGATGGACA TTGCGATCAT TGCTACAGAC CTTGCCTTGT ATTTCAAGAA AAGGACGATG 
TTCCAAAAGA TCGTGGATCA GTCTAAGACA TACGAGACTC AGCAGGAGTG GACGCAGTAC 
ATGATGCTGG ATCAGACACG GAAGGAAATT GTCATGTGCT GCCAAGCAGC TCTGATGAGT 
CATGATCCGA CCCTGCAACG TCAATGCGCT CCCTGCTTAT TGGATACACC TGCACTCCCC 
GGCATGGGAT TGACACCCAC AGCACATGAC CCTGGATTCC CCTCCCAGAC TTCACGCCGC 
TGTGCCCCAT CTCTGAAATA CCTTATCAAA TTCTCTCCTC TTGCTCCAAT TCTTTTCACA 
TGTCCCTTCC CTTTCTGTCT TATGTCATAC CTACCTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6A ( XM_010807419.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805721.2 Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235765
Clone ID Related Accession (Same CDS sequence)	XM_010807419.3
Accession Version	XM_010807419.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807419.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGAGG TGACGGCAGA GGAAGTAGAG AAGTTTCTGG ACTCAAATGT CAGCTTTGCC 
AAACAGTACT ACAACCTGCG CTACCGGGCC AAGGTCATCT CAGACCTGCT GGGACCCAGG 
GAGGCGGCCG TGGACTTCAG CAACTACCAT GCGCTGAACA GCGTGGAAGA GAGTGAAATT 
ATCTTCGACC TCCTGCGGGA CTTTCAGGAC AACCTGCAGG CCGAGAAGTG CGTCTTCAAT 
GTCATGAAGA AGTTGTGCTT CCTGCTGCAG GCCGACCGCA TGAGCCTATT CATGTACAGG 
GCCCGGAACG GCATCGCAGA GCTGGCCACC CGGCTCTTCA ACGTCCACAA GGATGCTGTG 
CTCGAGGAGT GCCTGGTGGC GCCTGACTCG GAGATCGTGT TCCCCCTGGA CATGGGAGTG 
GTGGGCCATG TTGCGCTCTC TAAAAAGATC GTCAACGTCC CCAACACAGA GGAGGATGAA 
CATTTCTGTG ACTTTGTGGA CACCCTCACA GAGTACCAGA CTAAGAACAT CTTGGCTTCT 
CCCATAATGA ATGGGAAGGA TGTGGTGGCC ATAATCATGG CTGTGAATAA AGTGGATGGG 
CCCCACTTCA CCGAGAATGA TGAAGAGATT CTTCTCAAGT ACCTCAATTT TGCAAATCTA 
ATCATGAAGG TGTTCCACCT GAGTTACCTG CACAACTGTG AGACTCGGCG TGGCCAGATA 
CTGTTGTGGT CTGGGAGCAA AGTCTTTGAA GAGCTTACAG ACATTGAGAG GCAGTTCCAC 
AAAGCCCTGT ACACAGTCCG CGCCTTCCTC AACTGTGACA GATACTCTGT GGGGCTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TCCTGATGGG GGAGGCTCCA 
CCCTACGCTG GTCCCAGGAC TCCGGATGGA AGGGAAATCA ACTTTTACAA GGTCATTGAC 
TACATCCTGC ATGGCAAAGA AGACATCAAA GTCATCCCGA ATCCTCCTCC AGACCACTGG 
GCTTTAGTGA GCGGTCTCCC CACTTATGTT GCCCAAAATG GCCTGATTTG CAACATCATG 
AATGCGCCTT CAGAGGACTT TTTTGCATTC CAGAAAGAGC CTCTGGATGA GTCTGGATGG 
GTGATTAAAA ATGTCCTTTC TATGCCAATT GTGAACAAGA AGGAGGAAAT TGTTGGGGTG 
GCCACGTTTT ACAATCGCAA AGATGGAAAA CCCTTTGATG AAATGGATGA GACCCTCATG 
GAGTCTTTGG CCCAGTTCCT GGGCTGGTCT GTCTTAAATC CTGACACTTA TGAGTTGATG 
AACAAACTTG AAAACAGGAA GGATATTTTC CAAGACATGG TGAAATACCA CGTGAAGTGT 
GACAATGAAG AAATCCAGAC AATCTTGAAA ACCAGAGAGG TGTATGGGAA GGAGCCGTGG 
GAATGCGAGG AAGAAGAACT GGCTGAGATC CTGCAAGGAG AGCTGCCAGA TGCAGACAAA 
TATGAAATTA ATAAATTCCA CTTCAGCGAC CTGCCCCTGA CGGAACTGGA GCTGGTGAAA 
TGTGGAATTC AGATGTACTA TGAGCTCAAA GTGGTGGATA AATTTCACAT TCCTCAGGAG 
GCCCTGGTGC GGTTCATGTA TTCCCTGAGT AAGGGCTACC GCAGGATCAC CTACCACAAC 
TGGCGGCACG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC CGGAAAGCTG 
AAGCGATACT TCACAGACCT GGAGGCCTTG GCCATGGTCA CCGCCGCCTT CTGCCATGAC 
ATTGACCACA GAGGCACTAA CAATCTCTAC CAGATGAAAT CCCAGAACCC ACTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGACAC CACTTGGAGT TTGGTAAAAC ACTCTTGAGA 
GATGAGAGCC TAAATATCTT TCAGAACCTC AATCGCAGGC AGCATGAGCA TGCAATCCAC 
ATGATGGACA TTGCGATCAT TGCTACAGAC CTTGCCTTGT ATTTCAAGAA AAGGACGATG 
TTCCAAAAGA TCGTGGATCA GTCTAAGACA TACGAGACTC AGCAGGAGTG GACGCAGTAC 
ATGATGCTGG ATCAGACACG GAAGGAAATT GTCATGTGCT GCCAAGCAGC TCTGATGAGT 
CATGATCCGA CCCTGCAACG TCAATGCGCT CCCTGCTTAT TGGATACACC TGCACTCCCC 
GGCATGGGAT TGACACCCAC AGCACATGAC CCTGGATTCC CCTCCCAGAC TTCACGCCGC 
TGTGCCCCAT CTCTGAAATA CCTTATCAAA TTCTCTCCTC TTGCTCCAAT TCTTTTCACA 
TGTCCCTTCC CTTTCTGTCT TATGTCATAC CTACCTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805721.2
CDS	298..2679
Translation	MGEVTAEEVEKFLDSNVSFAKQYYNLRYRAKVISDLLGPREAAVDFSNYHALNSVEESEIIFDLLRDFQDNLQAEKCVFNVMKKLCFLLQADRMSLFMYRARNGIAELATRLFNVHKDAVLEECLVAPDSEIVFPLDMGVVGHVALSKKIVNVPNTEEDEHFCDFVDTLTEYQTKNILASPIMNGKDVVAIIMAVNKVDGPHFTENDEEILLKYLNFANLIMKVFHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEAPPYAGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPPDHWALVSGLPTYVAQNGLICNIMNAPSEDFFAFQKEPLDESGWVIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEMDETLMESLAQFLGWSVLNPDTYELMNKLENRKDIFQDMVKYHVKCDNEEIQTILKTREVYGKEPWECEEEELAEILQGELPDADKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYETQQEWTQYMMLDQTRKEIVMCCQAALMSHDPTLQRQCAPCLLDTPALPGMGLTPTAHDPGFPSQTSRRCAPSLKYLIKFSPLAPILFTCPFPFCLMSYLPV

Target ORF information:

RefSeq Version	XM_010807419.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807419.3

ORF Insert Sequence:

ATGGGCGAGG TGACGGCAGA GGAAGTAGAG AAGTTTCTGG ACTCAAATGT CAGCTTTGCC 
AAACAGTACT ACAACCTGCG CTACCGGGCC AAGGTCATCT CAGACCTGCT GGGACCCAGG 
GAGGCGGCCG TGGACTTCAG CAACTACCAT GCGCTGAACA GCGTGGAAGA GAGTGAAATT 
ATCTTCGACC TCCTGCGGGA CTTTCAGGAC AACCTGCAGG CCGAGAAGTG CGTCTTCAAT 
GTCATGAAGA AGTTGTGCTT CCTGCTGCAG GCCGACCGCA TGAGCCTATT CATGTACAGG 
GCCCGGAACG GCATCGCAGA GCTGGCCACC CGGCTCTTCA ACGTCCACAA GGATGCTGTG 
CTCGAGGAGT GCCTGGTGGC GCCTGACTCG GAGATCGTGT TCCCCCTGGA CATGGGAGTG 
GTGGGCCATG TTGCGCTCTC TAAAAAGATC GTCAACGTCC CCAACACAGA GGAGGATGAA 
CATTTCTGTG ACTTTGTGGA CACCCTCACA GAGTACCAGA CTAAGAACAT CTTGGCTTCT 
CCCATAATGA ATGGGAAGGA TGTGGTGGCC ATAATCATGG CTGTGAATAA AGTGGATGGG 
CCCCACTTCA CCGAGAATGA TGAAGAGATT CTTCTCAAGT ACCTCAATTT TGCAAATCTA 
ATCATGAAGG TGTTCCACCT GAGTTACCTG CACAACTGTG AGACTCGGCG TGGCCAGATA 
CTGTTGTGGT CTGGGAGCAA AGTCTTTGAA GAGCTTACAG ACATTGAGAG GCAGTTCCAC 
AAAGCCCTGT ACACAGTCCG CGCCTTCCTC AACTGTGACA GATACTCTGT GGGGCTCTTA 
GACATGACCA AACAGAAGGA ATTTTTTGAT GTGTGGCCAG TCCTGATGGG GGAGGCTCCA 
CCCTACGCTG GTCCCAGGAC TCCGGATGGA AGGGAAATCA ACTTTTACAA GGTCATTGAC 
TACATCCTGC ATGGCAAAGA AGACATCAAA GTCATCCCGA ATCCTCCTCC AGACCACTGG 
GCTTTAGTGA GCGGTCTCCC CACTTATGTT GCCCAAAATG GCCTGATTTG CAACATCATG 
AATGCGCCTT CAGAGGACTT TTTTGCATTC CAGAAAGAGC CTCTGGATGA GTCTGGATGG 
GTGATTAAAA ATGTCCTTTC TATGCCAATT GTGAACAAGA AGGAGGAAAT TGTTGGGGTG 
GCCACGTTTT ACAATCGCAA AGATGGAAAA CCCTTTGATG AAATGGATGA GACCCTCATG 
GAGTCTTTGG CCCAGTTCCT GGGCTGGTCT GTCTTAAATC CTGACACTTA TGAGTTGATG 
AACAAACTTG AAAACAGGAA GGATATTTTC CAAGACATGG TGAAATACCA CGTGAAGTGT 
GACAATGAAG AAATCCAGAC AATCTTGAAA ACCAGAGAGG TGTATGGGAA GGAGCCGTGG 
GAATGCGAGG AAGAAGAACT GGCTGAGATC CTGCAAGGAG AGCTGCCAGA TGCAGACAAA 
TATGAAATTA ATAAATTCCA CTTCAGCGAC CTGCCCCTGA CGGAACTGGA GCTGGTGAAA 
TGTGGAATTC AGATGTACTA TGAGCTCAAA GTGGTGGATA AATTTCACAT TCCTCAGGAG 
GCCCTGGTGC GGTTCATGTA TTCCCTGAGT AAGGGCTACC GCAGGATCAC CTACCACAAC 
TGGCGGCACG GCTTCAACGT GGGGCAGACC ATGTTCTCCT TGCTGGTGAC CGGAAAGCTG 
AAGCGATACT TCACAGACCT GGAGGCCTTG GCCATGGTCA CCGCCGCCTT CTGCCATGAC 
ATTGACCACA GAGGCACTAA CAATCTCTAC CAGATGAAAT CCCAGAACCC ACTGGCCAAG 
CTCCATGGGT CCTCCATCTT GGAAAGACAC CACTTGGAGT TTGGTAAAAC ACTCTTGAGA 
GATGAGAGCC TAAATATCTT TCAGAACCTC AATCGCAGGC AGCATGAGCA TGCAATCCAC 
ATGATGGACA TTGCGATCAT TGCTACAGAC CTTGCCTTGT ATTTCAAGAA AAGGACGATG 
TTCCAAAAGA TCGTGGATCA GTCTAAGACA TACGAGACTC AGCAGGAGTG GACGCAGTAC 
ATGATGCTGG ATCAGACACG GAAGGAAATT GTCATGTGCT GCCAAGCAGC TCTGATGAGT 
CATGATCCGA CCCTGCAACG TCAATGCGCT CCCTGCTTAT TGGATACACC TGCACTCCCC 
GGCATGGGAT TGACACCCAC AGCACATGAC CCTGGATTCC CCTCCCAGAC TTCACGCCGC 
TGTGCCCCAT CTCTGAAATA CCTTATCAAA TTCTCTCCTC TTGCTCCAAT TCTTTTCACA 
TGTCCCTTCC CTTTCTGTCT TATGTCATAC CTACCTGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6A ( XM_024993841.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849609.1 Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235766
Clone ID Related Accession (Same CDS sequence)	XM_024993841.1
Accession Version	XM_024993841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGA AGGATGTGGT GGCCATAATC ATGGCTGTGA ATAAAGTGGA TGGGCCCCAC 
TTCACCGAGA ATGATGAAGA GATTCTTCTC AAGTACCTCA ATTTTGCAAA TCTAATCATG 
AAGGTGTTCC ACCTGAGTTA CCTGCACAAC TGTGAGACTC GGCGTGGCCA GATACTGTTG 
TGGTCTGGGA GCAAAGTCTT TGAAGAGCTT ACAGACATTG AGAGGCAGTT CCACAAAGCC 
CTGTACACAG TCCGCGCCTT CCTCAACTGT GACAGATACT CTGTGGGGCT CTTAGACATG 
ACCAAACAGA AGGAATTTTT TGATGTGTGG CCAGTCCTGA TGGGGGAGGC TCCACCCTAC 
GCTGGTCCCA GGACTCCGGA TGGAAGGGAA ATCAACTTTT ACAAGGTCAT TGACTACATC 
CTGCATGGCA AAGAAGACAT CAAAGTCATC CCGAATCCTC CTCCAGACCA CTGGGCTTTA 
GTGAGCGGTC TCCCCACTTA TGTTGCCCAA AATGGCCTGA TTTGCAACAT CATGAATGCG 
CCTTCAGAGG ACTTTTTTGC ATTCCAGAAA GAGCCTCTGG ATGAGTCTGG ATGGGTGATT 
AAAAATGTCC TTTCTATGCC AATTGTGAAC AAGAAGGAGG AAATTGTTGG GGTGGCCACG 
TTTTACAATC GCAAAGATGG AAAACCCTTT GATGAAATGG ATGAGACCCT CATGGAGTCT 
TTGGCCCAGT TCCTGGGCTG GTCTGTCTTA AATCCTGACA CTTATGAGTT GATGAACAAA 
CTTGAAAACA GGAAGGATAT TTTCCAAGAC ATGGTGAAAT ACCACGTGAA GTGTGACAAT 
GAAGAAATCC AGACAATCTT GAAAACCAGA GAGGTGTATG GGAAGGAGCC GTGGGAATGC 
GAGGAAGAAG AACTGGCTGA GATCCTGCAA GGAGAGCTGC CAGATGCAGA CAAATATGAA 
ATTAATAAAT TCCACTTCAG CGACCTGCCC CTGACGGAAC TGGAGCTGGT GAAATGTGGA 
ATTCAGATGT ACTATGAGCT CAAAGTGGTG GATAAATTTC ACATTCCTCA GGAGGCCCTG 
GTGCGGTTCA TGTATTCCCT GAGTAAGGGC TACCGCAGGA TCACCTACCA CAACTGGCGG 
CACGGCTTCA ACGTGGGGCA GACCATGTTC TCCTTGCTGG TGACCGGAAA GCTGAAGCGA 
TACTTCACAG ACCTGGAGGC CTTGGCCATG GTCACCGCCG CCTTCTGCCA TGACATTGAC 
CACAGAGGCA CTAACAATCT CTACCAGATG AAATCCCAGA ACCCACTGGC CAAGCTCCAT 
GGGTCCTCCA TCTTGGAAAG ACACCACTTG GAGTTTGGTA AAACACTCTT GAGAGATGAG 
AGCCTAAATA TCTTTCAGAA CCTCAATCGC AGGCAGCATG AGCATGCAAT CCACATGATG 
GACATTGCGA TCATTGCTAC AGACCTTGCC TTGTATTTCA AGAAAAGGAC GATGTTCCAA 
AAGATCGTGG ATCAGTCTAA GACATACGAG ACTCAGCAGG AGTGGACGCA GTACATGATG 
CTGGATCAGA CACGGAAGGA AATTGTCATG GCCATGATGA TGACCGCCTG TGATCTCTCA 
GCCATCACCA AGCCTTGGGA GGTGCAGAGC AAGGTGGCTC TGCTGGTTGC TGCTGAATTC 
TGGGAACAAG GTGACCTGGA GCGCACGGTG CTGCAACAGA ATCCCATTCC CATGATGGAC 
AGAAACAAGG CGGATGAACT CCCTAAGCTT CAGGTCGGCT TCATCGACTT TGTTTGCACC 
TTTGTCTACA AGGAATTCTC CCGTTTCCAC GAGGAGATCA CTCCCATGCT GGATGGGATT 
ACCAACAACC GCAAGGAGTG GAAAGCGCTT GCTGATGAGT ATGAGACCAA GATGAAGGGG 
CTGGAGGAGG AGAAGCAGAA ACAGCAGGCA GCCAACCAAG CAGCCGCAGG AAGTCAACAC 
GGCGGAAAGC AACCTGGGGG CGGGCCTGCA TCCAAGTCTT GCTGCGTCCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849609.1
CDS	206..2239
Translation	MNGKDVVAIIMAVNKVDGPHFTENDEEILLKYLNFANLIMKVFHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEAPPYAGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPPDHWALVSGLPTYVAQNGLICNIMNAPSEDFFAFQKEPLDESGWVIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEMDETLMESLAQFLGWSVLNPDTYELMNKLENRKDIFQDMVKYHVKCDNEEIQTILKTREVYGKEPWECEEEELAEILQGELPDADKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRRITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYETQQEWTQYMMLDQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYETKMKGLEEEKQKQQAANQAAAGSQHGGKQPGGGPASKSCCVQ

Target ORF information:

RefSeq Version	XM_024993841.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6A (PDE6A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993841.1

ORF Insert Sequence:

ATGAATGGGA AGGATGTGGT GGCCATAATC ATGGCTGTGA ATAAAGTGGA TGGGCCCCAC 
TTCACCGAGA ATGATGAAGA GATTCTTCTC AAGTACCTCA ATTTTGCAAA TCTAATCATG 
AAGGTGTTCC ACCTGAGTTA CCTGCACAAC TGTGAGACTC GGCGTGGCCA GATACTGTTG 
TGGTCTGGGA GCAAAGTCTT TGAAGAGCTT ACAGACATTG AGAGGCAGTT CCACAAAGCC 
CTGTACACAG TCCGCGCCTT CCTCAACTGT GACAGATACT CTGTGGGGCT CTTAGACATG 
ACCAAACAGA AGGAATTTTT TGATGTGTGG CCAGTCCTGA TGGGGGAGGC TCCACCCTAC 
GCTGGTCCCA GGACTCCGGA TGGAAGGGAA ATCAACTTTT ACAAGGTCAT TGACTACATC 
CTGCATGGCA AAGAAGACAT CAAAGTCATC CCGAATCCTC CTCCAGACCA CTGGGCTTTA 
GTGAGCGGTC TCCCCACTTA TGTTGCCCAA AATGGCCTGA TTTGCAACAT CATGAATGCG 
CCTTCAGAGG ACTTTTTTGC ATTCCAGAAA GAGCCTCTGG ATGAGTCTGG ATGGGTGATT 
AAAAATGTCC TTTCTATGCC AATTGTGAAC AAGAAGGAGG AAATTGTTGG GGTGGCCACG 
TTTTACAATC GCAAAGATGG AAAACCCTTT GATGAAATGG ATGAGACCCT CATGGAGTCT 
TTGGCCCAGT TCCTGGGCTG GTCTGTCTTA AATCCTGACA CTTATGAGTT GATGAACAAA 
CTTGAAAACA GGAAGGATAT TTTCCAAGAC ATGGTGAAAT ACCACGTGAA GTGTGACAAT 
GAAGAAATCC AGACAATCTT GAAAACCAGA GAGGTGTATG GGAAGGAGCC GTGGGAATGC 
GAGGAAGAAG AACTGGCTGA GATCCTGCAA GGAGAGCTGC CAGATGCAGA CAAATATGAA 
ATTAATAAAT TCCACTTCAG CGACCTGCCC CTGACGGAAC TGGAGCTGGT GAAATGTGGA 
ATTCAGATGT ACTATGAGCT CAAAGTGGTG GATAAATTTC ACATTCCTCA GGAGGCCCTG 
GTGCGGTTCA TGTATTCCCT GAGTAAGGGC TACCGCAGGA TCACCTACCA CAACTGGCGG 
CACGGCTTCA ACGTGGGGCA GACCATGTTC TCCTTGCTGG TGACCGGAAA GCTGAAGCGA 
TACTTCACAG ACCTGGAGGC CTTGGCCATG GTCACCGCCG CCTTCTGCCA TGACATTGAC 
CACAGAGGCA CTAACAATCT CTACCAGATG AAATCCCAGA ACCCACTGGC CAAGCTCCAT 
GGGTCCTCCA TCTTGGAAAG ACACCACTTG GAGTTTGGTA AAACACTCTT GAGAGATGAG 
AGCCTAAATA TCTTTCAGAA CCTCAATCGC AGGCAGCATG AGCATGCAAT CCACATGATG 
GACATTGCGA TCATTGCTAC AGACCTTGCC TTGTATTTCA AGAAAAGGAC GATGTTCCAA 
AAGATCGTGG ATCAGTCTAA GACATACGAG ACTCAGCAGG AGTGGACGCA GTACATGATG 
CTGGATCAGA CACGGAAGGA AATTGTCATG GCCATGATGA TGACCGCCTG TGATCTCTCA 
GCCATCACCA AGCCTTGGGA GGTGCAGAGC AAGGTGGCTC TGCTGGTTGC TGCTGAATTC 
TGGGAACAAG GTGACCTGGA GCGCACGGTG CTGCAACAGA ATCCCATTCC CATGATGGAC 
AGAAACAAGG CGGATGAACT CCCTAAGCTT CAGGTCGGCT TCATCGACTT TGTTTGCACC 
TTTGTCTACA AGGAATTCTC CCGTTTCCAC GAGGAGATCA CTCCCATGCT GGATGGGATT 
ACCAACAACC GCAAGGAGTG GAAAGCGCTT GCTGATGAGT ATGAGACCAA GATGAAGGGG 
CTGGAGGAGG AGAAGCAGAA ACAGCAGGCA GCCAACCAAG CAGCCGCAGG AAGTCAACAC 
GGCGGAAAGC AACCTGGGGG CGGGCCTGCA TCCAAGTCTT GCTGCGTCCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Domain organization and conformational plasticity of the G protein effector, PDE6.
The Journal of biological chemistry290(20)12833-43(2015 May)
Zhang Z,He F,Constantine R,Baker ML,Baehr W,Schmid MF,Wensel TG,Agosto MA

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Direct allosteric regulation between the GAF domain and catalytic domain of photoreceptor phosphodiesterase PDE6.
The Journal of biological chemistry283(44)29699-705(2008 Oct)
Zhang XJ,Cahill KB,Elfenbein A,Arshavsky VY,Cote RH

Glutamic acid-rich proteins of rod photoreceptors are natively unfolded.
The Journal of biological chemistry281(3)1449-60(2006 Jan)
Batra-Safferling R,Abarca-Heidemann K,K?rschen HG,Tziatzios C,Stoldt M,Budyak I,Willbold D,Schwalbe H,Klein-Seetharaman J,Kaupp UB

Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.
The Journal of biological chemistry280(13)12585-92(2005 Apr)
Guo LW,Grant JE,Hajipour AR,Muradov H,Arbabian M,Artemyev NO,Ruoho AE

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PDE6A cDNA ORF clone, Bos taurus(cattle)

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