CHRNE cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNE
Entrez Gene ID	281688
Full Name	cholinergic receptor nicotinic epsilon subunit
General protein information	Preferred Names cholinergic receptor nicotinic epsilon subunit Names acetylcholine receptor subunit epsilon AchR epsilon subunit cholinergic receptor, nicotinic, epsilon (muscle) cholinergic receptor, nicotinic, epsilon polypeptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010815894.2	XP_010814196.1	acetylcholine receptor subunit epsilon isoform X1
XM_015458609.2	XP_015314095.1	acetylcholine receptor subunit epsilon isoform X2
XM_015458608.1	XP_015314094.1	acetylcholine receptor subunit epsilon isoform X2
XM_010815894.3	XP_010814196.1	acetylcholine receptor subunit epsilon isoform X1
XM_015458609.1	XP_015314095.1	acetylcholine receptor subunit epsilon isoform X3
NM_174272.2	NP_776697.1	acetylcholine receptor subunit epsilon precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRNE	NP_000071.1
Rattus norvegicus (Norway rat)	Chrne	NP_058890.1
Xenopus tropicalis (tropical clawed frog)	chrne	XP_002941867.2
Pan troglodytes (chimpanzee)	CHRNE	XP_523556.4
Canis lupus familiaris (dog)	CHRNE	XP_536608.3
Bos taurus (cattle)	CHRNE	NP_776697.1
	CHRNE	XP_001097094.1
Mus musculus (house mouse)	Chrne	NP_033733.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The CHRNE 470del20 mutation causing congenital myasthenic syndrome in South African Brahman cattle: prevalence, origin, and association with performance traits.
Thompson PN, van der Werf JH, Heesterbeek JA, van Arendonk JA
Journal of animal science85(3)604-9(2007 Mar)

Congenital myasthenic syndrome in cattle due to homozygosity for a truncating mutation in the acetylcholine receptor (AChR) epsilon-subunit gene.
Sieb JP, Kraner S, Thompson PN, Steinlein OK
Annals of the New York Academy of Sciences998125-7(2003 Sep)

Congenital myasthenia in Brahman calves caused by homozygosity for a CHRNE truncating mutation.
Kraner S, Sieb JP, Thompson PN, Steinlein OK
Neurogenetics4(2)87-91(2002 Oct)

Overlapping of MINK and CHRNE gene loci in the course of mammalian evolution.
Dan I, Watanabe NM, Kajikawa E, Ishida T, Pandey A, Kusumi A
Nucleic acids research30(13)2906-10(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genotyping of the South African, registered, Brahman cattle population for the 470del20 mutation in the CHRNE gene causing congenital myasthenic syndrome is reported.
Title: The CHRNE 470del20 mutation causing congenital myasthenic syndrome in South African Brahman cattle: prevalence, origin, and association with performance traits.

It was found that mutations within muscle AChRs are the most common cause of CMS. The majority are located within the epsilon-subunit gene and result in AChR deficiency.
Title: Congenital myasthenic syndrome in cattle due to homozygosity for a truncating mutation in the acetylcholine receptor (AChR) epsilon-subunit gene.

To elucidate the genetic defect in four previously reported related Brahman calves with severe myasthenic weakness, we determined the genomic structure of the gene encoding the bovine epsilon-subunit (bovCHRNE) of the acetylcholine receptor (AChR).
Title: Congenital myasthenia in Brahman calves caused by homozygosity for a CHRNE truncating mutation.

The following CHRNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41510	NM_174272.2 Latest version!	Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa45202	XM_010815894.2 Latest version!	Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa225973	XM_015458609.2 Latest version!	Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa93801	XM_015458608.1 Latest version!	Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa93802	XM_015458609.1 Latest version!	Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa225972	XM_010815894.3 Latest version!	Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNE ( NM_174272.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776697.1 Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.

Price & Availability↑

CloneID	OBa41510
Clone ID Related Accession (Same CDS sequence)	NM_174272.2
Accession Version	NM_174272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	acetylcholine receptor subunit epsilon precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X02597.1. On Jun 3, 2003 this sequence version replaced NM_174272.1. ##Evidence-Data-START## Transcript exon combination :: X02597.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145426 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGAGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGC TACCCTCTGC CAGAACCTGG GCGCTGCCGC CCCCGAGATC 
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCTTCAAGCA CTCGGGACCA GGAGGCCACT 
GGCGAGGAGG TGTCCGACTG GGTGCGCATG GGGAAGGCCC TTGACAGCAT CTGCTTCTGG 
GCTGCTCTGG TGCTCTTCCT CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
CGAGTGCCTC AGCTGCCCTA CCCGCCATGT ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776697.1
CDS	23..1498
Translation	MAGALLCALLLLQLLGRGEGKNEELRLYHYLFDTYDPGRRPVQEPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKGDFGGVETLRVPSELVWLPEIVLENNIDGQFGVAYEANVLVSEGGYLSWLPPAIYRSTCAVEVTYFPFDWQNCSLVFRSQTYNAEEVEFVFAVDDEGKTISKIDIDTEAYTENGEWAIDFCPGVIRRHDGDSAGGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKTPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHAMSPRLRYVLLELLPQLLGSGAPPEIPRAASPPRRASSLGLLLRAEELILKKPRSELVFEQQRHRHGTWTATLCQNLGAAAPEIRCCVDAVNFVASSTRDQEATGEEVSDWVRMGKALDSICFWAALVLFLVGSSLIFLGAYFNRVPQLPYPPCM

Target ORF information:

RefSeq Version	NM_174272.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174272.2

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGAGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGC TACCCTCTGC CAGAACCTGG GCGCTGCCGC CCCCGAGATC 
CGCTGCTGTG TGGATGCCGT GAACTTCGTG GCTTCAAGCA CTCGGGACCA GGAGGCCACT 
GGCGAGGAGG TGTCCGACTG GGTGCGCATG GGGAAGGCCC TTGACAGCAT CTGCTTCTGG 
GCTGCTCTGG TGCTCTTCCT CGTGGGCTCC AGCCTCATCT TCCTCGGGGC CTACTTCAAC 
CGAGTGCCTC AGCTGCCCTA CCCGCCATGT ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNE ( XM_010815894.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814196.1 Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45202
Clone ID Related Accession (Same CDS sequence)	XM_010815894.2
Accession Version	XM_010815894.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	acetylcholine receptor subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815894.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG TGAGCGGACT GGCCCGAGTG GGCCAAGGGG GGCGCCTTCA 
AGGGCTAGGT TCTTGATCAC CCACCCCGGG TCGCCCCCAC CAGCTACCCT CTGCCAGAAC 
CTGGGCGCTG CCGCCCCCGA GATCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCTTCA 
AGCACTCGGG ACCAGGAGGC CACTGGCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG 
GCCCTTGACA GCATCTGCTT CTGGGCTGCT CTGGTGCTCT TCCTCGTGGG CTCCAGCCTC 
ATCTTCCTCG GGGCCTACTT CAACCGAGTG CCTCAGCTGC CCTACCCGCC ATGTATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814196.1
CDS	796..2355
Translation	MAGALLCALLLLQLLGRGEGKNEELRLYHYLFDTYDPGRRPVQEPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKGDFGGVETLRVPSELVWLPEIVLENNIDGQFGVAYEANVLVSEGGYLSWLPPAIYRSTCAVEVTYFPFDWQNCSLVFRSQTYNAEEVEFVFAVDDEGKTISKIDIDTEAYTENGEWAIDFCPGVIRRHDGDSAGGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKTPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHAMSPRLRYVLLELLPQLLGSGAPPEIPRAASPPRRASSLGLLLRAEELILKKPRSELVFEQQRHRHGTWTGERTGPSGPRGAPSRARFLITHPGSPPPATLCQNLGAAAPEIRCCVDAVNFVASSTRDQEATGEEVSDWVRMGKALDSICFWAALVLFLVGSSLIFLGAYFNRVPQLPYPPCM

Target ORF information:

RefSeq Version	XM_010815894.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815894.2

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG TGAGCGGACT GGCCCGAGTG GGCCAAGGGG GGCGCCTTCA 
AGGGCTAGGT TCTTGATCAC CCACCCCGGG TCGCCCCCAC CAGCTACCCT CTGCCAGAAC 
CTGGGCGCTG CCGCCCCCGA GATCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCTTCA 
AGCACTCGGG ACCAGGAGGC CACTGGCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG 
GCCCTTGACA GCATCTGCTT CTGGGCTGCT CTGGTGCTCT TCCTCGTGGG CTCCAGCCTC 
ATCTTCCTCG GGGCCTACTT CAACCGAGTG CCTCAGCTGC CCTACCCGCC ATGTATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNE ( XM_015458609.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314095.1 Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa225973
Clone ID Related Accession (Same CDS sequence)	XM_015458609.2
Accession Version	XM_015458609.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acetylcholine receptor subunit epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458609.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGAGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG AGGTGTCCGA CTGGGTGCGC ATGGGGAAGG CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314095.1
CDS	74..1330
Translation	MAGALLCALLLLQLLGRGEGKNEELRLYHYLFDTYDPGRRPVQEPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKGDFGGVETLRVPSELVWLPEIVLENNIDGQFGVAYEANVLVSEGGYLSWLPPAIYRSTCAVEVTYFPFDWQNCSLVFRSQTYNAEEVEFVFAVDDEGKTISKIDIDTEAYTENGEWAIDFCPGVIRRHDGDSAGGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKTPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHAMSPRLRYVLLELLPQLLGSGAPPEIPRAASPPRRASSLGLLLRAEELILKKPRSELVFEQQRHRHGTWTGGVRLGAHGEGP

Target ORF information:

RefSeq Version	XM_015458609.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458609.2

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGAGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG AGGTGTCCGA CTGGGTGCGC ATGGGGAAGG CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNE ( XM_015458608.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314094.1 Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa93801
Clone ID Related Accession (Same CDS sequence)	XM_015458608.1
Accession Version	XM_015458608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	acetylcholine receptor subunit epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG TGAGCGGACT GGCCCGAGTG GGCCAAGGGG GGCGCCTTCA 
AGGGCTAGGT TCTTGATCAC CCACCCCGGG TCGCCCCCAC CAGCTACCCT CTGCCAGAAC 
CTGGGCGCTG CCGCCCCCGA GATCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCTTCA 
AGCACTCGGG ACCAGGAGGC CACTGGCGAG CTTTTAGGGA CTCAATTTGT GCCCACCCCT 
TCCCCAGGAG GTGTCCGACT GGGTGCGCAT GGGGAAGGCC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314094.1
CDS	799..2283
Translation	MAGALLCALLLLQLLGRGEGKNEELRLYHYLFDTYDPGRRPVQEPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKGDFGGVETLRVPSELVWLPEIVLENNIDGQFGVAYEANVLVSEGGYLSWLPPAIYRSTCAVEVTYFPFDWQNCSLVFRSQTYNAEEVEFVFAVDDEGKTISKIDIDTEAYTENGEWAIDFCPGVIRRHDGDSAGGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKTPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHAMSPRLRYVLLELLPQLLGSGAPPEIPRAASPPRRASSLGLLLRAEELILKKPRSELVFEQQRHRHGTWTGERTGPSGPRGAPSRARFLITHPGSPPPATLCQNLGAAAPEIRCCVDAVNFVASSTRDQEATGELLGTQFVPTPSPGGVRLGAHGEGP

Target ORF information:

RefSeq Version	XM_015458608.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458608.1

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG TGAGCGGACT GGCCCGAGTG GGCCAAGGGG GGCGCCTTCA 
AGGGCTAGGT TCTTGATCAC CCACCCCGGG TCGCCCCCAC CAGCTACCCT CTGCCAGAAC 
CTGGGCGCTG CCGCCCCCGA GATCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCTTCA 
AGCACTCGGG ACCAGGAGGC CACTGGCGAG CTTTTAGGGA CTCAATTTGT GCCCACCCCT 
TCCCCAGGAG GTGTCCGACT GGGTGCGCAT GGGGAAGGCC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNE ( XM_015458609.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314095.1 Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa93802
Clone ID Related Accession (Same CDS sequence)	XM_015458609.1
Accession Version	XM_015458609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	acetylcholine receptor subunit epsilon isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG AGGTGTCCGA CTGGGTGCGC ATGGGGAAGG CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314095.1
CDS	797..2053
Translation	MAGALLCALLLLQLLGRGEGKNEELRLYHYLFDTYDPGRRPVQEPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKGDFGGVETLRVPSELVWLPEIVLENNIDGQFGVAYEANVLVSEGGYLSWLPPAIYRSTCAVEVTYFPFDWQNCSLVFRSQTYNAEEVEFVFAVDDEGKTISKIDIDTEAYTENGEWAIDFCPGVIRRHDGDSAGGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKTPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHAMSPRLRYVLLELLPQLLGSGAPPEIPRAASPPRRASSLGLLLRAEELILKKPRSELVFEQQRHRHGTWTGGVRLGAHGEGP

Target ORF information:

RefSeq Version	XM_015458609.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cholinergic receptor, nicotinic epsilon (CHRNE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458609.1

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGCGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG AGGTGTCCGA CTGGGTGCGC ATGGGGAAGG CCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRNE ( XM_010815894.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814196.1 Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa225972
Clone ID Related Accession (Same CDS sequence)	XM_010815894.3
Accession Version	XM_010815894.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	acetylcholine receptor subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGAGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG TGAGCGGACT GGCCCGAGTG GGCCAAGGGG GGCGCCTTCA 
AGGGCTAGGT TCTTGATCAC CCACCCCGGG TCGCCCCCAC CAGCTACCCT CTGCCAGAAC 
CTGGGCGCTG CCGCCCCCGA GATCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCTTCA 
AGCACTCGGG ACCAGGAGGC CACTGGCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG 
GCCCTTGACA GCATCTGCTT CTGGGCTGCT CTGGTGCTCT TCCTCGTGGG CTCCAGCCTC 
ATCTTCCTCG GGGCCTACTT CAACCGAGTG CCTCAGCTGC CCTACCCGCC ATGTATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814196.1
CDS	74..1633
Translation	MAGALLCALLLLQLLGRGEGKNEELRLYHYLFDTYDPGRRPVQEPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKGDFGGVETLRVPSELVWLPEIVLENNIDGQFGVAYEANVLVSEGGYLSWLPPAIYRSTCAVEVTYFPFDWQNCSLVFRSQTYNAEEVEFVFAVDDEGKTISKIDIDTEAYTENGEWAIDFCPGVIRRHDGDSAGGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVLLAQTVFLFLIAQKTPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPTTHAMSPRLRYVLLELLPQLLGSGAPPEIPRAASPPRRASSLGLLLRAEELILKKPRSELVFEQQRHRHGTWTGERTGPSGPRGAPSRARFLITHPGSPPPATLCQNLGAAAPEIRCCVDAVNFVASSTRDQEATGEEVSDWVRMGKALDSICFWAALVLFLVGSSLIFLGAYFNRVPQLPYPPCM

Target ORF information:

RefSeq Version	XM_010815894.3
Organism	Bos taurus(cattle)
Definition	Bos taurus cholinergic receptor nicotinic epsilon subunit (CHRNE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815894.3

ORF Insert Sequence:

ATGGCAGGGG CTCTGCTCTG CGCCCTGCTC CTCTTGCAGC TCCTCGGCAG AGGTGAGGGG 
AAGAATGAGG AGCTGCGCCT TTACCACTAC CTCTTCGACA CCTATGACCC AGGACGCCGG 
CCAGTGCAGG AGCCCGAGGA CACTGTCACC ATCTCCCTCA AAGTCACCCT GACCAACCTC 
ATCTCCCTGA ACGAGAAAGA AGAGACCCTC ACCACCAGCG TCTGGATTGG AATCGACTGG 
CAAGATTACC GACTCAACTA CAGCAAGGGC GACTTTGGGG GCGTAGAAAC CCTGAGGGTC 
CCTTCAGAAC TCGTATGGCT GCCAGAGATT GTGCTGGAAA ACAATATCGA CGGCCAGTTC 
GGGGTGGCCT ACGAGGCCAA TGTGCTGGTC TCTGAGGGCG GCTACTTGAG CTGGTTGCCC 
CCGGCCATCT ACCGCAGTAC CTGCGCCGTG GAGGTCACCT ACTTCCCTTT CGACTGGCAG 
AACTGCTCGC TCGTTTTCCG CTCCCAGACG TACAATGCCG AAGAGGTGGA GTTTGTCTTT 
GCGGTGGACG ACGAGGGCAA GACCATCAGC AAGATCGATA TCGATACTGA GGCCTATACT 
GAGAACGGCG AGTGGGCCAT CGACTTCTGT CCCGGGGTGA TCCGCCGCCA CGACGGTGAC 
TCTGCGGGTG GCCCAGGGGA AACTGACGTC ATCTACTCGC TCATTATTCG CCGGAAGCCA 
CTCTTCTACG TTATTAACAT CATCGTGCCC TGCGTGCTCA TCTCGGGCCT GGTGCTGCTC 
GCCTACTTCC TGCCCGCGCA GGCCGGCGGC CAGAAATGCA CCGTTTCCAT CAACGTCCTG 
CTCGCCCAGA CCGTCTTCTT GTTCCTGATT GCCCAGAAAA CCCCAGAGAC ATCTCTGAGC 
GTGCCGCTGT TGGGCAGGTA CCTCATTTTC GTCATGGTGG TCGCCACACT CATTGTCATG 
AATTGCGTCA TCGTGCTCAA CGTGTCATTG CGGACGCCCA CCACTCACGC CATGTCCCCG 
CGACTGCGCT ACGTGTTGCT GGAGCTGCTA CCCCAGCTCC TGGGCTCCGG CGCGCCCCCC 
GAGATCCCCC GAGCTGCCTC GCCCCCAAGG AGGGCGTCGT CCCTGGGCCT CCTGCTCCGC 
GCGGAGGAGC TGATACTGAA AAAGCCGCGG AGCGAACTGG TATTTGAGCA GCAGAGGCAC 
CGGCACGGAA CCTGGACGGG TGAGCGGACT GGCCCGAGTG GGCCAAGGGG GGCGCCTTCA 
AGGGCTAGGT TCTTGATCAC CCACCCCGGG TCGCCCCCAC CAGCTACCCT CTGCCAGAAC 
CTGGGCGCTG CCGCCCCCGA GATCCGCTGC TGTGTGGATG CCGTGAACTT CGTGGCTTCA 
AGCACTCGGG ACCAGGAGGC CACTGGCGAG GAGGTGTCCG ACTGGGTGCG CATGGGGAAG 
GCCCTTGACA GCATCTGCTT CTGGGCTGCT CTGGTGCTCT TCCTCGTGGG CTCCAGCCTC 
ATCTTCCTCG GGGCCTACTT CAACCGAGTG CCTCAGCTGC CCTACCCGCC ATGTATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The CHRNE 470del20 mutation causing congenital myasthenic syndrome in South African Brahman cattle: prevalence, origin, and association with performance traits.
Journal of animal science85(3)604-9(2007 Mar)
Thompson PN,van der Werf JH,Heesterbeek JA,van Arendonk JA

Congenital myasthenic syndrome in cattle due to homozygosity for a truncating mutation in the acetylcholine receptor (AChR) epsilon-subunit gene.
Annals of the New York Academy of Sciences998125-7(2003 Sep)
Sieb JP,Kraner S,Thompson PN,Steinlein OK

Congenital myasthenia in Brahman calves caused by homozygosity for a CHRNE truncating mutation.
Neurogenetics4(2)87-91(2002 Oct)
Kraner S,Sieb JP,Thompson PN,Steinlein OK

Overlapping of MINK and CHRNE gene loci in the course of mammalian evolution.
Nucleic acids research30(13)2906-10(2002 Jul)
Dan I,Watanabe NM,Kajikawa E,Ishida T,Pandey A,Kusumi A

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CHRNE cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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