APEX1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	APEX1
Entrez Gene ID	281630
Full Name	apurinic/apyrimidinic endodeoxyribonuclease 1
Synonyms	APEN,BAP1,REF-1
General protein information	Preferred Names apurinic/apyrimidinic endodeoxyribonuclease 1 Names DNA-(apurinic or apyrimidinic site) lyase AP endonuclease 1 AP lyase APEX nuclease (multifunctional DNA repair enzyme) 1 apurinic-apyrimidinic endonuclease 1 apurinic/apyrimidinic endonuclease redox factor-1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_176609.3	NP_788782.2	DNA-(apurinic or apyrimidinic site) lyase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	APEX1	NP_001138591.1
Mus musculus (house mouse)	Apex1	NP_033817.1
Gallus gallus (chicken)	APEX1	NP_001171688.1
Arabidopsis thaliana (thale cress)	ARP	NP_181677.1
Pan troglodytes (chimpanzee)	APEX1	NP_001074954.1
	APEX1	XP_001090240.1
Rattus norvegicus (Norway rat)	Apex1	NP_077062.1
Oryza sativa (rice)	Os01g0801000	NP_001044537.1
Bos taurus (cattle)	APEX1	NP_788782.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009587	XP_318609.4
Xenopus tropicalis (tropical clawed frog)	apex1	NP_989307.1
Homo sapiens (human)	APEX1	NP_001231178.1
Danio rerio (zebrafish)	apex1	NP_998586.1

Gene Ontology
Bibliography

Related articles in PubMed

Meiosis progression and donor age affect expression profile of DNA repair genes in bovine oocytes.
Bilotto S, Boni R, Russo GL, Lioi MB
Zygote (Cambridge, England)23(1)11-8(2015 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Three-dimensional structure prediction of bovine AP lyase, BAP1: prediction of interaction with DNA and alterations as a result of Arg176-->Ala, Asp282-->Ala, and His308-->Asn mutations.
Khurshid R, Salim A, Abbasi A
Biochemical and biophysical research communications326(4)711-7(2005 Jan)

Isolation of cDNA clones encoding an enzyme from bovine cells that repairs oxidative DNA damage in vitro: homology with bacterial repair enzymes.
Robson CN, Milne AM, Pappin DJ, Hickson ID
Nucleic acids research19(5)1087-92(1991 Mar)

Purification and amino-terminal amino acid sequence of an apurinic/apyrimidinic endonuclease from calf thymus.
Henner WD, Kiker NP, Jorgensen TJ, Munck JN
Nucleic acids research15(14)5529-44(1987 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Meiosis progression and female age affect expression profile of DNA repair APEX1 gene in bovine oocytes.
Title: Meiosis progression and donor age affect expression profile of DNA repair genes in bovine oocytes.

prediction of the 3D structure of bovine AP lyase (BAP1); models of mutants showed substitution of Arg176-->Ala leads to the loss of DNA binding whereas mutation of Asp282-->Ala and His308-->Asn leads to a decrease in the enzymatic activity.
Title: Three-dimensional structure prediction of bovine AP lyase, BAP1: prediction of interaction with DNA and alterations as a result of Arg176-->Ala, Asp282-->Ala, and His308-->Asn mutations.

The following APEX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APEX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01480	NM_176609.3 Latest version!	Bos taurus apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APEX1 ( NM_176609.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_788782.2 Bos taurus apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1), mRNA.

Price & Availability↑

CloneID	OBa01480
Clone ID Related Accession (Same CDS sequence)	NM_176609.3
Accession Version	NM_176609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Bos taurus(cattle)
Product	DNA-(apurinic or apyrimidinic site) lyase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH148795.1, DV824401.1, BC122610.1, DN548789.1 and X56685.1. On Feb 11, 2009 this sequence version replaced NM_176609.2. ##Evidence-Data-START## Transcript exon combination :: BC122610.1, DV920016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145433, SAMN03145437 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGAAAC GTGGGAAAAA GGGAGCGGTG GTCGAAGACG CGGAAGAGCC CAAGACTGAG 
CCAGAGGCGA AGAAGAGTAA GGCAGGAGCG AAAAAGAACG AAAAAGAGGC AGTAGGAGAG 
GGCGCAGTTC TGTATGAAGA CCCCCCAGAT CAGAAAACCT CACCCAGTGG CAAATCAGCC 
ACACTCAAGA TCTGCTCGTG GAATGTGGAT GGGCTTCGAG CCTGGATTAA GAAGAAAGGT 
TTAGATTGGG TAAAGGAAGA AGCCCCAGAC ATCCTGTGCC TCCAAGAGAC CAAATGTTCA 
GAGAACAAAC TACCAGTTGA ACTTCAAGAA CTGTCTGGAT TGTCCCATCA GTACTGGTCA 
GCTCCTTCAG ACAAGGAAGG GTACAGTGGT GTGGGCCTCC TCTCCCGCCA GTGCCCACTC 
AAAGTCTCTT ATGGCATTGG TGAGGAAGAA CATGATCAGG AAGGCCGAGT GATTGTGGCT 
GAATATGACG CATTTGTGCT GGTGACAGCC TATGTGCCTA ATGCAGGCCG AGGTCTGGTG 
CGCCTGGAGT ATCGCCAGCG CTGGGATGAA GCTTTTCGCA AATTCCTGAA GGGTTTGGCA 
TCCCGCAAGC CCCTTGTGCT ATGTGGGGAC CTCAATGTGG CTCATGAAGA AATTGACCTT 
CGCAACCCGA AGGGAAATAA AAAGAATGCT GGCTTCACTC CACAAGAGCG GCAGGGCTTC 
GGGGAATTGC TGCAGGCTGT GCCACTCACT GACAGTTTCC GGCATCTCTA CCCCAACACC 
GCCTATGCCT ACACCTTCTG GACCTATATG ATGAATGCGC GATCCAAAAA CGTTGGTTGG 
CGCCTTGATT ATTTTTTGTT ATCTCAGTCT CTGTTGCCTG CATTGTGTGA CAGTAAAATC 
CGTTCCAAGG CTCTGGGCAG TGACCACTGT CCCATTACCC TATACCTAGC TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788782.2
CDS	163..1119
Translation	MPKRGKKGAVVEDAEEPKTEPEAKKSKAGAKKNEKEAVGEGAVLYEDPPDQKTSPSGKSATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCSENKLPVELQELSGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGEEEHDQEGRVIVAEYDAFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLTDSFRHLYPNTAYAYTFWTYMMNARSKNVGWRLDYFLLSQSLLPALCDSKIRSKALGSDHCPITLYLAL

Target ORF information:

RefSeq Version	NM_176609.3
Organism	Bos taurus(cattle)
Definition	Bos taurus apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176609.3

ORF Insert Sequence:

ATGCCGAAAC GTGGGAAAAA GGGAGCGGTG GTCGAAGACG CGGAAGAGCC CAAGACTGAG 
CCAGAGGCGA AGAAGAGTAA GGCAGGAGCG AAAAAGAACG AAAAAGAGGC AGTAGGAGAG 
GGCGCAGTTC TGTATGAAGA CCCCCCAGAT CAGAAAACCT CACCCAGTGG CAAATCAGCC 
ACACTCAAGA TCTGCTCGTG GAATGTGGAT GGGCTTCGAG CCTGGATTAA GAAGAAAGGT 
TTAGATTGGG TAAAGGAAGA AGCCCCAGAC ATCCTGTGCC TCCAAGAGAC CAAATGTTCA 
GAGAACAAAC TACCAGTTGA ACTTCAAGAA CTGTCTGGAT TGTCCCATCA GTACTGGTCA 
GCTCCTTCAG ACAAGGAAGG GTACAGTGGT GTGGGCCTCC TCTCCCGCCA GTGCCCACTC 
AAAGTCTCTT ATGGCATTGG TGAGGAAGAA CATGATCAGG AAGGCCGAGT GATTGTGGCT 
GAATATGACG CATTTGTGCT GGTGACAGCC TATGTGCCTA ATGCAGGCCG AGGTCTGGTG 
CGCCTGGAGT ATCGCCAGCG CTGGGATGAA GCTTTTCGCA AATTCCTGAA GGGTTTGGCA 
TCCCGCAAGC CCCTTGTGCT ATGTGGGGAC CTCAATGTGG CTCATGAAGA AATTGACCTT 
CGCAACCCGA AGGGAAATAA AAAGAATGCT GGCTTCACTC CACAAGAGCG GCAGGGCTTC 
GGGGAATTGC TGCAGGCTGT GCCACTCACT GACAGTTTCC GGCATCTCTA CCCCAACACC 
GCCTATGCCT ACACCTTCTG GACCTATATG ATGAATGCGC GATCCAAAAA CGTTGGTTGG 
CGCCTTGATT ATTTTTTGTT ATCTCAGTCT CTGTTGCCTG CATTGTGTGA CAGTAAAATC 
CGTTCCAAGG CTCTGGGCAG TGACCACTGT CCCATTACCC TATACCTAGC TCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Meiosis progression and donor age affect expression profile of DNA repair genes in bovine oocytes.
Zygote (Cambridge, England)23(1)11-8(2015 Feb)
Bilotto S,Boni R,Russo GL,Lioi MB

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Three-dimensional structure prediction of bovine AP lyase, BAP1: prediction of interaction with DNA and alterations as a result of Arg176-->Ala, Asp282-->Ala, and His308-->Asn mutations.
Biochemical and biophysical research communications326(4)711-7(2005 Jan)
Khurshid R,Salim A,Abbasi A

Isolation of cDNA clones encoding an enzyme from bovine cells that repairs oxidative DNA damage in vitro: homology with bacterial repair enzymes.
Nucleic acids research19(5)1087-92(1991 Mar)
Robson CN,Milne AM,Pappin DJ,Hickson ID

Purification and amino-terminal amino acid sequence of an apurinic/apyrimidinic endonuclease from calf thymus.
Nucleic acids research15(14)5529-44(1987 Jul)
Henner WD,Kiker NP,Jorgensen TJ,Munck JN

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APEX1 cDNA ORF clone, Bos taurus(cattle)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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