TLR3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TLR3
Entrez Gene ID	281535
Full Name	toll like receptor 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008664.1	NP_001008664.1	toll-like receptor 3 precursor
XM_010820242.3	XP_010818544.2	toll-like receptor 3 isoform X1
XM_010820242.2	XP_010818544.1	toll-like receptor 3 isoform X1
XM_005225957.3	XP_005226014.1	toll-like receptor 3 isoform X2
XM_010820244.2	XP_010818546.1	toll-like receptor 3 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TLR3	NP_001008664.1
Danio rerio (zebrafish)	tlr3	NP_001013287.2
Xenopus tropicalis (tropical clawed frog)	tlr3	XP_002934448.2
Homo sapiens (human)	TLR3	NP_003256.1
	TLR3	NP_001031762.1
Gallus gallus (chicken)	TLR3	NP_001011691.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012385	XP_551799.2
Pan troglodytes (chimpanzee)	TLR3	NP_001123942.1
Canis lupus familiaris (dog)	TLR3	XP_005630025.1
Rattus norvegicus (Norway rat)	Tlr3	NP_942086.1
Mus musculus (house mouse)	Tlr3	NP_569054.2
Drosophila melanogaster (fruit fly)	Tollo	NP_524757.1

Gene Ontology
Bibliography

Related articles in PubMed

Toll-like receptors 3, 7 and 8 are upregulated in the placental caruncle and fetal spleen of Neospora caninum experimentally infected cattle.
Marin MS, Hecker YP, Quintana S, P?rez SE, Leunda MR, Cant?n GJ, Cobo ER, Moore DP, Ode?n AC
Veterinary parasitology23658-61(2017 Mar)

Involvement of toll-like receptors 3 and 7/8 in the neuropathogenesis of bovine herpesvirus types 1 and 5.
Rensetti D, Marin M, Quintana S, Mor?n P, Verna A, Ode?n A, P?rez S
Research in veterinary science1071-7(2016 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Detection of polymorphisms in bovine toll-like receptors 3, 7, 8, and 9.
Cargill EJ, Womack JE
Genomics89(6)745-55(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

On day 104 of pregnancy, mRNA expression of TLRs 3 and 8, as well as that of TLRs 7 and 9, was high in the spleen of fetuses from Neospora caninum-infected heifers. Gene expression levels of endosomal TLRs were also detectable in the placenta and the maternal caruncle from infected heifers, being TLRs 3, 7 and 8 particularly upregulated, mostly in the caruncle.
Title: Toll-like receptors 3, 7 and 8 are upregulated in the placental caruncle and fetal spleen of Neospora caninum experimentally infected cattle.

The results from this study demonstrate that expression of at least TLR3, TLR7 and TLR8 is stimulated upon bovine alpha-herpesvirus infection of the brain.
Title: Involvement of toll-like receptors 3 and 7/8 in the neuropathogenesis of bovine herpesvirus types 1 and 5.

Comparative sequence analysis revealed a total of 139 polymorphisms, which include single-nucleotide polymorphisms and insertion-deletion polymorphisms.
Title: Detection of polymorphisms in bovine toll-like receptors 3, 7, 8, and 9.

The following TLR3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43623	NM_001008664.1 Latest version!	Bos taurus toll like receptor 3 (TLR3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa33895	XM_010820242.2 Latest version!	Bos taurus toll like receptor 3 (TLR3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa33896	XM_010820244.2 Latest version!	Bos taurus toll like receptor 3 (TLR3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa33896	XM_005225957.3 Latest version!	Bos taurus toll like receptor 3 (TLR3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa231300	XM_010820242.3 Latest version!	Bos taurus toll like receptor 3 (TLR3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TLR3 ( NM_001008664.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001008664.1 Bos taurus toll like receptor 3 (TLR3), mRNA.

Price & Availability↑

CloneID	OBa43623
Clone ID Related Accession (Same CDS sequence)	NM_001008664.1
Accession Version	NM_001008664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	toll-like receptor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ812026.1. ##Evidence-Data-START## Transcript exon combination :: AJ812026.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAGAC CTTTGCCTTA TCATATCCAC TTCTTTTCGG GACTGTTGAC CTGTTGGATA 
CTGTGTACAT CTTCTGCCCA CAAATGTACT GTTAGACATG AAGTAGCTGA TTGCAGTCAT 
CTGAAGTTGA CGCAAATACC TGATGACCTC CCAACAAACA TAACTGTGTT GAATCTCACC 
CATAATCAAC TCAGAAGATT GCCACCTGCC AATTTTACGA GATACAGCCA ACTTACTACC 
TTGGATGGGG GATTTAACTC CATCTCAAAG CTGGAGCCAG AACTGTGTCA AAGTCTCCCT 
TGGTTGGAAA TTTTGAACCT CCAACACAAT GAGATATCAC AGCTTTCTGA TAAAACCTTT 
ATCTTCTGCA TGAATTTGAC TGAACTCCAT CTAATGTCCA ACTCAATCCA AAAAATTAAA 
AACGATCCCT TTAAAAACCT GAAGAATTTA ATCAAATTAG ACCTCTCTCA TAATGGCTTA 
TCATCTACTA AATTAGGAAC TCAGCTCCAA CTGGAGAACC TTCAAGAGCT TCTATTATCA 
AATAATAAAA TTTCTTCACT AACACCAGGA GAATTTGACT TCCTTGGCAA TTCTTCTTTA 
AAAAGATTAG AGTTGTCATC AAATCAAATC AAAGAGTTCT CTCCTGGGTG TTTTCATACC 
CTTGGAGAAT TATCTGGCCT CTCTCTGAAC AATGCCAAGC TGAGCCCCAG TCTCACAGAG 
AAGCTCTGCT TGGAACTGTC AAACACAAGC ATTGAGAATC TGTCCCTGAG CAGCAACCAG 
CTGGACACAA TCAGCCACAC GACCTTCGAT GGACTGAAGC AGACGAATCT CACCACGCTG 
GACCTTTCCC GTAACTCCTT ACGTGTGATG GGTAATGACT CCTTTGCCTG GCTTCCACAT 
CTCGAATACC TCTCTCTGGA GTATAATAAC ATAGAGCATT TGTCTTCTCG CTCTTTTTAT 
GGGCTTTCCA ACTTGAGACG CCTGGACTTG AGACGGTCTT TTACTAGACA AAGCATTTCA 
CTGACTTCGC TCCCCAAGAT TGACGATTTT TCCTTTCAGT GGCTAAAATG TTTGGAGTAC 
CTCAACATGG ACGATAACAA CTTTCCAGGC ATAAAAAGAA ATACTTTCAC GGGATTGGTG 
AGGCTGAAAT TTTTAAGTCT CTCCAACTCC TTCTCAAGTT TGCGGACTTT AACAAATGAG 
ACATTTCTAT CGCTCGCTGG TTGTCCTCTG CTCCTACTCG ACCTAACCAA AAATAAAATC 
TCAAAAATTC AGAGTGGTGC TTTTTCTTGG TTGGGGCACC TGGAGGTCCT TGACCTCGGC 
CTTAATGAGA TTGGGCAAGA ACTCACAGGC CAGGAATGGA GAGGCCTAGA CAATATTGTC 
GAAATCTACC TTTCCTACAA CAAATACCTA GAGCTGACCA CCAACTCTTT CACCTCAGTT 
CCAAGCCTTC AAAGACTGAT GCTCCGAAGG GTGGCCCTGA AAAATGTGGA CTGCTCCCCT 
TCGCCTTTTC GCCCTCTTCC GAACCTGGTC ATTCTGGATC TAAGCAACAA CAACATAGCC 
AACATAAATG ACGAGTTGCT GAAGGGTCTT GAGAAACTAG AAATTCTGGA CTTGCAGCAT 
AACAACTTAG CTCGGCTCTG GAAGCATGCC AACCCTGGCG GCCCTGTTCA GTTTCTGAAG 
GGCCTTTTTC ACCTCCACAT CCTTAACTTA GGGTCTAATG GCTTTGATGA GATCCCAGTG 
GAAGCCTTCA AGGACTTACG TGAATTGAAG AGTATTGATT TAGGAATGAA TAATTTAAAT 
ATCCTTCCAC AATCTGTCTT TGATAATCAA GTGTCACTGA AGTCATTAAG CCTTCAGAAG 
AACCTCATAA CATCTGTTCA AAAGACTGTT TTTGGGCCAG CGTTCAGAAA CCTGAGTTAT 
TTAGATATGC GTTTTAATCC ATTTGATTGT ACCTGTGAAA GCATTGCCTG GTTTGTTAAT 
TGGATTAATA TTACCCACAC CAACATCTCT GAACTGTCGA ACCATTACCT CTGCAACACT 
CCGCCCCAAT ACCATGGTTA CCCAGTAATG CTTTTCGATG TATCACCCTG CAAAGACAGC 
GCCCCATTTG AACTCCTTTT CATGATTAAC ATCAATATCC TTTTGATTTT TATCTTTATT 
GTACTGCTCA TCCATTTTGA AGGCTGGAGG ATATCTTTTT ATTGGAATGT TTCAGTGCAT 
CGAGTTCTCG GTTTCAAAGA AATAGACAGA GCAGAGCAGT TTGAATATGC AGCATATATA 
ATTCATGCCT ATAAAGATAG GGATTGGGTC TGGAAGCACT CCTCCCCAAT GGAGGACGAA 
GATCATACTC TCAGATTTTG TCTGGAAGAA AGGGACTTTG AGGCAGGTGT CCTTGAACTT 
GAAGCAATTG TGAACAGCAT CAGAAGGAGC AGAAAAATTA TTTTCGTTGT AACACAGAAT 
CTATTGAAAG ATCCACTATG CAAAAGATTC AAGGTTCACC ATGCAGTTCA GCAAGCTATT 
GAACAAAATC TGGATTCCAT TATATTGATC TTTCTTGAGG AGATTCCGGA TTATAAACTG 
AACCATGCAC TCTGTTTGCG AAGAGGGATG TTTAAATCTC ATTGCATCTT GAATTGGCCG 
GTTCAGAAGG AACGGGTAAA TGCTTTTCAT CATAAATTGA AAGTAGCACT TGGGTCCAGA 
AATTCGGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008664.1
CDS	115..2829
Translation	MSRPLPYHIHFFSGLLTCWILCTSSAHKCTVRHEVADCSHLKLTQIPDDLPTNITVLNLTHNQLRRLPPANFTRYSQLTTLDGGFNSISKLEPELCQSLPWLEILNLQHNEISQLSDKTFIFCMNLTELHLMSNSIQKIKNDPFKNLKNLIKLDLSHNGLSSTKLGTQLQLENLQELLLSNNKISSLTPGEFDFLGNSSLKRLELSSNQIKEFSPGCFHTLGELSGLSLNNAKLSPSLTEKLCLELSNTSIENLSLSSNQLDTISHTTFDGLKQTNLTTLDLSRNSLRVMGNDSFAWLPHLEYLSLEYNNIEHLSSRSFYGLSNLRRLDLRRSFTRQSISLTSLPKIDDFSFQWLKCLEYLNMDDNNFPGIKRNTFTGLVRLKFLSLSNSFSSLRTLTNETFLSLAGCPLLLLDLTKNKISKIQSGAFSWLGHLEVLDLGLNEIGQELTGQEWRGLDNIVEIYLSYNKYLELTTNSFTSVPSLQRLMLRRVALKNVDCSPSPFRPLPNLVILDLSNNNIANINDELLKGLEKLEILDLQHNNLARLWKHANPGGPVQFLKGLFHLHILNLGSNGFDEIPVEAFKDLRELKSIDLGMNNLNILPQSVFDNQVSLKSLSLQKNLITSVQKTVFGPAFRNLSYLDMRFNPFDCTCESIAWFVNWINITHTNISELSNHYLCNTPPQYHGYPVMLFDVSPCKDSAPFELLFMININILLIFIFIVLLIHFEGWRISFYWNVSVHRVLGFKEIDRAEQFEYAAYIIHAYKDRDWVWKHSSPMEDEDHTLRFCLEERDFEAGVLELEAIVNSIRRSRKIIFVVTQNLLKDPLCKRFKVHHAVQQAIEQNLDSIILIFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKERVNAFHHKLKVALGSRNSAH

Target ORF information:

RefSeq Version	NM_001008664.1
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 3 (TLR3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008664.1

ORF Insert Sequence:

ATGAGCAGAC CTTTGCCTTA TCATATCCAC TTCTTTTCGG GACTGTTGAC CTGTTGGATA 
CTGTGTACAT CTTCTGCCCA CAAATGTACT GTTAGACATG AAGTAGCTGA TTGCAGTCAT 
CTGAAGTTGA CGCAAATACC TGATGACCTC CCAACAAACA TAACTGTGTT GAATCTCACC 
CATAATCAAC TCAGAAGATT GCCACCTGCC AATTTTACGA GATACAGCCA ACTTACTACC 
TTGGATGGGG GATTTAACTC CATCTCAAAG CTGGAGCCAG AACTGTGTCA AAGTCTCCCT 
TGGTTGGAAA TTTTGAACCT CCAACACAAT GAGATATCAC AGCTTTCTGA TAAAACCTTT 
ATCTTCTGCA TGAATTTGAC TGAACTCCAT CTAATGTCCA ACTCAATCCA AAAAATTAAA 
AACGATCCCT TTAAAAACCT GAAGAATTTA ATCAAATTAG ACCTCTCTCA TAATGGCTTA 
TCATCTACTA AATTAGGAAC TCAGCTCCAA CTGGAGAACC TTCAAGAGCT TCTATTATCA 
AATAATAAAA TTTCTTCACT AACACCAGGA GAATTTGACT TCCTTGGCAA TTCTTCTTTA 
AAAAGATTAG AGTTGTCATC AAATCAAATC AAAGAGTTCT CTCCTGGGTG TTTTCATACC 
CTTGGAGAAT TATCTGGCCT CTCTCTGAAC AATGCCAAGC TGAGCCCCAG TCTCACAGAG 
AAGCTCTGCT TGGAACTGTC AAACACAAGC ATTGAGAATC TGTCCCTGAG CAGCAACCAG 
CTGGACACAA TCAGCCACAC GACCTTCGAT GGACTGAAGC AGACGAATCT CACCACGCTG 
GACCTTTCCC GTAACTCCTT ACGTGTGATG GGTAATGACT CCTTTGCCTG GCTTCCACAT 
CTCGAATACC TCTCTCTGGA GTATAATAAC ATAGAGCATT TGTCTTCTCG CTCTTTTTAT 
GGGCTTTCCA ACTTGAGACG CCTGGACTTG AGACGGTCTT TTACTAGACA AAGCATTTCA 
CTGACTTCGC TCCCCAAGAT TGACGATTTT TCCTTTCAGT GGCTAAAATG TTTGGAGTAC 
CTCAACATGG ACGATAACAA CTTTCCAGGC ATAAAAAGAA ATACTTTCAC GGGATTGGTG 
AGGCTGAAAT TTTTAAGTCT CTCCAACTCC TTCTCAAGTT TGCGGACTTT AACAAATGAG 
ACATTTCTAT CGCTCGCTGG TTGTCCTCTG CTCCTACTCG ACCTAACCAA AAATAAAATC 
TCAAAAATTC AGAGTGGTGC TTTTTCTTGG TTGGGGCACC TGGAGGTCCT TGACCTCGGC 
CTTAATGAGA TTGGGCAAGA ACTCACAGGC CAGGAATGGA GAGGCCTAGA CAATATTGTC 
GAAATCTACC TTTCCTACAA CAAATACCTA GAGCTGACCA CCAACTCTTT CACCTCAGTT 
CCAAGCCTTC AAAGACTGAT GCTCCGAAGG GTGGCCCTGA AAAATGTGGA CTGCTCCCCT 
TCGCCTTTTC GCCCTCTTCC GAACCTGGTC ATTCTGGATC TAAGCAACAA CAACATAGCC 
AACATAAATG ACGAGTTGCT GAAGGGTCTT GAGAAACTAG AAATTCTGGA CTTGCAGCAT 
AACAACTTAG CTCGGCTCTG GAAGCATGCC AACCCTGGCG GCCCTGTTCA GTTTCTGAAG 
GGCCTTTTTC ACCTCCACAT CCTTAACTTA GGGTCTAATG GCTTTGATGA GATCCCAGTG 
GAAGCCTTCA AGGACTTACG TGAATTGAAG AGTATTGATT TAGGAATGAA TAATTTAAAT 
ATCCTTCCAC AATCTGTCTT TGATAATCAA GTGTCACTGA AGTCATTAAG CCTTCAGAAG 
AACCTCATAA CATCTGTTCA AAAGACTGTT TTTGGGCCAG CGTTCAGAAA CCTGAGTTAT 
TTAGATATGC GTTTTAATCC ATTTGATTGT ACCTGTGAAA GCATTGCCTG GTTTGTTAAT 
TGGATTAATA TTACCCACAC CAACATCTCT GAACTGTCGA ACCATTACCT CTGCAACACT 
CCGCCCCAAT ACCATGGTTA CCCAGTAATG CTTTTCGATG TATCACCCTG CAAAGACAGC 
GCCCCATTTG AACTCCTTTT CATGATTAAC ATCAATATCC TTTTGATTTT TATCTTTATT 
GTACTGCTCA TCCATTTTGA AGGCTGGAGG ATATCTTTTT ATTGGAATGT TTCAGTGCAT 
CGAGTTCTCG GTTTCAAAGA AATAGACAGA GCAGAGCAGT TTGAATATGC AGCATATATA 
ATTCATGCCT ATAAAGATAG GGATTGGGTC TGGAAGCACT CCTCCCCAAT GGAGGACGAA 
GATCATACTC TCAGATTTTG TCTGGAAGAA AGGGACTTTG AGGCAGGTGT CCTTGAACTT 
GAAGCAATTG TGAACAGCAT CAGAAGGAGC AGAAAAATTA TTTTCGTTGT AACACAGAAT 
CTATTGAAAG ATCCACTATG CAAAAGATTC AAGGTTCACC ATGCAGTTCA GCAAGCTATT 
GAACAAAATC TGGATTCCAT TATATTGATC TTTCTTGAGG AGATTCCGGA TTATAAACTG 
AACCATGCAC TCTGTTTGCG AAGAGGGATG TTTAAATCTC ATTGCATCTT GAATTGGCCG 
GTTCAGAAGG AACGGGTAAA TGCTTTTCAT CATAAATTGA AAGTAGCACT TGGGTCCAGA 
AATTCGGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR3 ( XM_010820242.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818544.1 Bos taurus toll like receptor 3 (TLR3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa33895
Clone ID Related Accession (Same CDS sequence)	XM_010820242.2
Accession Version	XM_010820242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	toll-like receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAG GAACGGTCAG CTGTCGTCAC AGCAAAAGCA TGAGCAGACC TTTGCCTTAT 
CATATCCACT TCTTTTCGGG ACTGTTGACC TGTTGGATAC TGTGTACATC TTCTGCCCAC 
AAATGTACTG TTAGACATGA AGTAGCTGAT TGCAGTCATC TGAAGTTGAC GCAAATACCT 
GATGACCTCC CAACAAACAT AACTGTGTTG AATCTCACCC ATAATCAACT CAGAAGATTG 
CCACCTGCCA ATTTTACGAG ATACAGCCAA CTTACTATCT TGGATGGGGG ATTTAACTCC 
ATCTCAAAGC TGGAGCCAGA ACTGTGTCAA AGTCTCCCTT GGTTGGAAAT TTTGAACCTC 
CAACACAATG AGATATCTCA GCTTTCTGAT AAAACCTTTA TCTTCTGCAT GAATTTGACT 
GAACTCCATC TAATGTCCAA CTCAATCCAA AAAATTAAAA ACGATCCCTT TAAAAACCTG 
AAGAATTTAA TCAAATTAGA CCTCTCTCAT AATGGCTTAT CATCTACTAA ATTAGGAACT 
CAGCTCCAAC TGGAGAACCT CCAAGAGCTT CTATTATCAA ATAATAAAAT TTCTTCACTA 
ACACCAGAAG AATTTGATTT CCTTGGCAAT TCTTCTTTAA AAAGATTAGA GTTGTCATCA 
AATCAAATCA AAGAGTTCTC TCCTGGGTGT TTTCATACCC TTGGAGAATT ATCTGGCCTC 
TCTCTGAACA ATGCCAAGCT GAGCCCCAGT CTCACAGAGA AGCTCTGCTT GGAACTGTCA 
AACACAAGCA TTGAGAATCT GTCCCTGAGC AGCAACCAGC TGGACACAAT CAGCCACACG 
ACCTTCGATG GACTGAAGCA GACGAATCTC ACCACGCTGG ACCTTTCCCG TAACTCCTTA 
CGTGTGATGG GTAATGACTC CTTTGCCTGG CTTCCACATC TCGAATACCT CTCTCTGGAG 
TATAATAACA TAGAGCATTT GTCTTCTCGC TCTTTTTATG GGCTTTCCAA CTTGAGACGC 
CTGGACTTGA GACGGTCTTT TACTAGACAA AGCATTTCAC TGACTTCGCT CCCCAAGATT 
GACGATTTTT CCTTTCAGTG GCTAAAATGT TTGGAGTACC TCAACATGGA CGATAACAAC 
TTTCCAGGCA TAAAAAGAAA TACTTTCACG GGATTGGTGA GGCTGAAATT TTTAAGTCTC 
TCCAACTCCT TCTCAAGTTT GCGGACTTTA ACAAATGAGA CATTTCTATC GCTCGCTGGT 
TGTCCTCTGC TCCTACTCAA CCTAACCAAA AATAAAATCT CAAAAATTCA GAGTGGTGCT 
TTTTCTTGGT TGGGGCACCT GGAGGTCCTT GACCTCGGCC TTAATGAGAT TGGGCAAGAA 
CTCACAGGCC AGGAATGGAG AGGCCTAGAC AATATTGTCG AAATCTACCT TTCCTACAAC 
AAATACCTAG AGCTGACCAC CAACTCTTTC ACCTCAGTTC CAAGCCTTCA AAGACTGATG 
CTCCGAAGGG TGGCCCTGAA AAATGTGGAC TGCTCCCCTT CGCCTTTTCG CCCTCTTCCG 
AACCTGGTCA TTCTGGATCT AAGCAACAAC AACATAGCCA ACATAAATGA CGAGTTGCTG 
AAGGGTCTTG AGAAACTAGA AATTCTGGAC TTGCAGCATA ACAACTTAGC TCGGCTCTGG 
AAGCATGCCA ACCCTGGCGG CCCTGTTCAG TTTCTGAAGG GCCTTTTTCA CCTCCACATC 
CTTAACTTAG GGTCTAATGG CTTTGATGAG ATCCCAGTGG AAGCCTTCAA GGACTTACGT 
GAATTGAAGA GTATTGATTT AGGAATGAAT AATTTAAATA TCCTTCCACA ATCTGTCTTT 
GATAATCAAG TGTCACTGAA GTCATTAAGC CTTCAGAAGA ACCTCATAAC ATCTGTTCAA 
AAGACTGTTT TTGGGCCAGC GTTCAGGAAC CTGAGTTATT TAGATATGCG TTTTAATCCA 
TTTGATTGTA CCTGTGAAAG CATTGCCTGG TTTGTTAATT GGATTAATAG TACCCACACC 
AACATCTCTG AACTGTCGAA CCATTACCTC TGCAACACTC CGCCCCAATA CCATGGTTAC 
CCAGTAATGC TTTTCGATGT ATCGCCCTGC AAAGACAGCG CCCCATTTGA ACTCCTTTTC 
ATGATTAACA TCAATATCCT TTTGATTTTT ATCTTTATTG TACTGCTCAT CCATTTTGAA 
GGCTGGAGGA TATCTTTTTA TTGGAATGTT TCAGTGCATC GAGTTCTCGG TTTCAAAGAA 
ATAGACAGAG CAGAGCAGTT TGAATATGCA GCATATATAA TTCATGCCTA TAAAGATAGG 
GATTGGGTCT GGAAGCACTT CTCCCCAATG GAGGAAGAAG ATCATACTCT CAGATTTTGT 
CTGGAAGAAA GGGACTTTGA GGCAGGTGTC CTTGAACTTG AAGCAATTGT GAACAGCATC 
AGAAGGAGCA GAAAAATTAT TTTCGTTGTA ACACAGAATC TATTGAAAGA TCCACTATGC 
AAAAGATTCA AGGTTCACCA TGCAGTTCAG CAAGCTATTG AACAAAATCT GGATTCCATT 
ATATTGATCT TTCTTGAGGA GATTCCGGAT TATAAACTGA ACCATGCACT CTGTTTGCGA 
AGAGGGATGT TTAAATCTCA TTGCATCTTG AATTGGCCGG TTCAGAAAGA ACGGGTAAAT 
GCTTTTCATC ATAAATTGAA AGTAGCACTT GGGTCCAGAA ATTCAGCACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818544.1
CDS	351..3104
Translation	MKKGTVSCRHSKSMSRPLPYHIHFFSGLLTCWILCTSSAHKCTVRHEVADCSHLKLTQIPDDLPTNITVLNLTHNQLRRLPPANFTRYSQLTILDGGFNSISKLEPELCQSLPWLEILNLQHNEISQLSDKTFIFCMNLTELHLMSNSIQKIKNDPFKNLKNLIKLDLSHNGLSSTKLGTQLQLENLQELLLSNNKISSLTPEEFDFLGNSSLKRLELSSNQIKEFSPGCFHTLGELSGLSLNNAKLSPSLTEKLCLELSNTSIENLSLSSNQLDTISHTTFDGLKQTNLTTLDLSRNSLRVMGNDSFAWLPHLEYLSLEYNNIEHLSSRSFYGLSNLRRLDLRRSFTRQSISLTSLPKIDDFSFQWLKCLEYLNMDDNNFPGIKRNTFTGLVRLKFLSLSNSFSSLRTLTNETFLSLAGCPLLLLNLTKNKISKIQSGAFSWLGHLEVLDLGLNEIGQELTGQEWRGLDNIVEIYLSYNKYLELTTNSFTSVPSLQRLMLRRVALKNVDCSPSPFRPLPNLVILDLSNNNIANINDELLKGLEKLEILDLQHNNLARLWKHANPGGPVQFLKGLFHLHILNLGSNGFDEIPVEAFKDLRELKSIDLGMNNLNILPQSVFDNQVSLKSLSLQKNLITSVQKTVFGPAFRNLSYLDMRFNPFDCTCESIAWFVNWINSTHTNISELSNHYLCNTPPQYHGYPVMLFDVSPCKDSAPFELLFMININILLIFIFIVLLIHFEGWRISFYWNVSVHRVLGFKEIDRAEQFEYAAYIIHAYKDRDWVWKHFSPMEEEDHTLRFCLEERDFEAGVLELEAIVNSIRRSRKIIFVVTQNLLKDPLCKRFKVHHAVQQAIEQNLDSIILIFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKERVNAFHHKLKVALGSRNSAH

Target ORF information:

RefSeq Version	XM_010820242.2
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 3 (TLR3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820242.2

ORF Insert Sequence:

ATGAAAAAAG GAACGGTCAG CTGTCGTCAC AGCAAAAGCA TGAGCAGACC TTTGCCTTAT 
CATATCCACT TCTTTTCGGG ACTGTTGACC TGTTGGATAC TGTGTACATC TTCTGCCCAC 
AAATGTACTG TTAGACATGA AGTAGCTGAT TGCAGTCATC TGAAGTTGAC GCAAATACCT 
GATGACCTCC CAACAAACAT AACTGTGTTG AATCTCACCC ATAATCAACT CAGAAGATTG 
CCACCTGCCA ATTTTACGAG ATACAGCCAA CTTACTATCT TGGATGGGGG ATTTAACTCC 
ATCTCAAAGC TGGAGCCAGA ACTGTGTCAA AGTCTCCCTT GGTTGGAAAT TTTGAACCTC 
CAACACAATG AGATATCTCA GCTTTCTGAT AAAACCTTTA TCTTCTGCAT GAATTTGACT 
GAACTCCATC TAATGTCCAA CTCAATCCAA AAAATTAAAA ACGATCCCTT TAAAAACCTG 
AAGAATTTAA TCAAATTAGA CCTCTCTCAT AATGGCTTAT CATCTACTAA ATTAGGAACT 
CAGCTCCAAC TGGAGAACCT CCAAGAGCTT CTATTATCAA ATAATAAAAT TTCTTCACTA 
ACACCAGAAG AATTTGATTT CCTTGGCAAT TCTTCTTTAA AAAGATTAGA GTTGTCATCA 
AATCAAATCA AAGAGTTCTC TCCTGGGTGT TTTCATACCC TTGGAGAATT ATCTGGCCTC 
TCTCTGAACA ATGCCAAGCT GAGCCCCAGT CTCACAGAGA AGCTCTGCTT GGAACTGTCA 
AACACAAGCA TTGAGAATCT GTCCCTGAGC AGCAACCAGC TGGACACAAT CAGCCACACG 
ACCTTCGATG GACTGAAGCA GACGAATCTC ACCACGCTGG ACCTTTCCCG TAACTCCTTA 
CGTGTGATGG GTAATGACTC CTTTGCCTGG CTTCCACATC TCGAATACCT CTCTCTGGAG 
TATAATAACA TAGAGCATTT GTCTTCTCGC TCTTTTTATG GGCTTTCCAA CTTGAGACGC 
CTGGACTTGA GACGGTCTTT TACTAGACAA AGCATTTCAC TGACTTCGCT CCCCAAGATT 
GACGATTTTT CCTTTCAGTG GCTAAAATGT TTGGAGTACC TCAACATGGA CGATAACAAC 
TTTCCAGGCA TAAAAAGAAA TACTTTCACG GGATTGGTGA GGCTGAAATT TTTAAGTCTC 
TCCAACTCCT TCTCAAGTTT GCGGACTTTA ACAAATGAGA CATTTCTATC GCTCGCTGGT 
TGTCCTCTGC TCCTACTCAA CCTAACCAAA AATAAAATCT CAAAAATTCA GAGTGGTGCT 
TTTTCTTGGT TGGGGCACCT GGAGGTCCTT GACCTCGGCC TTAATGAGAT TGGGCAAGAA 
CTCACAGGCC AGGAATGGAG AGGCCTAGAC AATATTGTCG AAATCTACCT TTCCTACAAC 
AAATACCTAG AGCTGACCAC CAACTCTTTC ACCTCAGTTC CAAGCCTTCA AAGACTGATG 
CTCCGAAGGG TGGCCCTGAA AAATGTGGAC TGCTCCCCTT CGCCTTTTCG CCCTCTTCCG 
AACCTGGTCA TTCTGGATCT AAGCAACAAC AACATAGCCA ACATAAATGA CGAGTTGCTG 
AAGGGTCTTG AGAAACTAGA AATTCTGGAC TTGCAGCATA ACAACTTAGC TCGGCTCTGG 
AAGCATGCCA ACCCTGGCGG CCCTGTTCAG TTTCTGAAGG GCCTTTTTCA CCTCCACATC 
CTTAACTTAG GGTCTAATGG CTTTGATGAG ATCCCAGTGG AAGCCTTCAA GGACTTACGT 
GAATTGAAGA GTATTGATTT AGGAATGAAT AATTTAAATA TCCTTCCACA ATCTGTCTTT 
GATAATCAAG TGTCACTGAA GTCATTAAGC CTTCAGAAGA ACCTCATAAC ATCTGTTCAA 
AAGACTGTTT TTGGGCCAGC GTTCAGGAAC CTGAGTTATT TAGATATGCG TTTTAATCCA 
TTTGATTGTA CCTGTGAAAG CATTGCCTGG TTTGTTAATT GGATTAATAG TACCCACACC 
AACATCTCTG AACTGTCGAA CCATTACCTC TGCAACACTC CGCCCCAATA CCATGGTTAC 
CCAGTAATGC TTTTCGATGT ATCGCCCTGC AAAGACAGCG CCCCATTTGA ACTCCTTTTC 
ATGATTAACA TCAATATCCT TTTGATTTTT ATCTTTATTG TACTGCTCAT CCATTTTGAA 
GGCTGGAGGA TATCTTTTTA TTGGAATGTT TCAGTGCATC GAGTTCTCGG TTTCAAAGAA 
ATAGACAGAG CAGAGCAGTT TGAATATGCA GCATATATAA TTCATGCCTA TAAAGATAGG 
GATTGGGTCT GGAAGCACTT CTCCCCAATG GAGGAAGAAG ATCATACTCT CAGATTTTGT 
CTGGAAGAAA GGGACTTTGA GGCAGGTGTC CTTGAACTTG AAGCAATTGT GAACAGCATC 
AGAAGGAGCA GAAAAATTAT TTTCGTTGTA ACACAGAATC TATTGAAAGA TCCACTATGC 
AAAAGATTCA AGGTTCACCA TGCAGTTCAG CAAGCTATTG AACAAAATCT GGATTCCATT 
ATATTGATCT TTCTTGAGGA GATTCCGGAT TATAAACTGA ACCATGCACT CTGTTTGCGA 
AGAGGGATGT TTAAATCTCA TTGCATCTTG AATTGGCCGG TTCAGAAAGA ACGGGTAAAT 
GCTTTTCATC ATAAATTGAA AGTAGCACTT GGGTCCAGAA ATTCAGCACA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR3 ( XM_005225957.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226014.1 Bos taurus toll like receptor 3 (TLR3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa33896
Clone ID Related Accession (Same CDS sequence)	XM_005225957.3 , XM_010820244.2
Accession Version	XM_005225957.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	toll-like receptor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAC CTTTGCCTTA TCATATCCAC TTCTTTTCGG GACTGTTGAC CTGTTGGATA 
CTGTGTACAT CTTCTGCCCA CAAATGTACT GTTAGACATG AAGTAGCTGA TTGCAGTCAT 
CTGAAGTTGA CGCAAATACC TGATGACCTC CCAACAAACA TAACTGTGTT GAATCTCACC 
CATAATCAAC TCAGAAGATT GCCACCTGCC AATTTTACGA GATACAGCCA ACTTACTATC 
TTGGATGGGG GATTTAACTC CATCTCAAAG CTGGAGCCAG AACTGTGTCA AAGTCTCCCT 
TGGTTGGAAA TTTTGAACCT CCAACACAAT GAGATATCTC AGCTTTCTGA TAAAACCTTT 
ATCTTCTGCA TGAATTTGAC TGAACTCCAT CTAATGTCCA ACTCAATCCA AAAAATTAAA 
AACGATCCCT TTAAAAACCT GAAGAATTTA ATCAAATTAG ACCTCTCTCA TAATGGCTTA 
TCATCTACTA AATTAGGAAC TCAGCTCCAA CTGGAGAACC TCCAAGAGCT TCTATTATCA 
AATAATAAAA TTTCTTCACT AACACCAGAA GAATTTGATT TCCTTGGCAA TTCTTCTTTA 
AAAAGATTAG AGTTGTCATC AAATCAAATC AAAGAGTTCT CTCCTGGGTG TTTTCATACC 
CTTGGAGAAT TATCTGGCCT CTCTCTGAAC AATGCCAAGC TGAGCCCCAG TCTCACAGAG 
AAGCTCTGCT TGGAACTGTC AAACACAAGC ATTGAGAATC TGTCCCTGAG CAGCAACCAG 
CTGGACACAA TCAGCCACAC GACCTTCGAT GGACTGAAGC AGACGAATCT CACCACGCTG 
GACCTTTCCC GTAACTCCTT ACGTGTGATG GGTAATGACT CCTTTGCCTG GCTTCCACAT 
CTCGAATACC TCTCTCTGGA GTATAATAAC ATAGAGCATT TGTCTTCTCG CTCTTTTTAT 
GGGCTTTCCA ACTTGAGACG CCTGGACTTG AGACGGTCTT TTACTAGACA AAGCATTTCA 
CTGACTTCGC TCCCCAAGAT TGACGATTTT TCCTTTCAGT GGCTAAAATG TTTGGAGTAC 
CTCAACATGG ACGATAACAA CTTTCCAGGC ATAAAAAGAA ATACTTTCAC GGGATTGGTG 
AGGCTGAAAT TTTTAAGTCT CTCCAACTCC TTCTCAAGTT TGCGGACTTT AACAAATGAG 
ACATTTCTAT CGCTCGCTGG TTGTCCTCTG CTCCTACTCA ACCTAACCAA AAATAAAATC 
TCAAAAATTC AGAGTGGTGC TTTTTCTTGG TTGGGGCACC TGGAGGTCCT TGACCTCGGC 
CTTAATGAGA TTGGGCAAGA ACTCACAGGC CAGGAATGGA GAGGCCTAGA CAATATTGTC 
GAAATCTACC TTTCCTACAA CAAATACCTA GAGCTGACCA CCAACTCTTT CACCTCAGTT 
CCAAGCCTTC AAAGACTGAT GCTCCGAAGG GTGGCCCTGA AAAATGTGGA CTGCTCCCCT 
TCGCCTTTTC GCCCTCTTCC GAACCTGGTC ATTCTGGATC TAAGCAACAA CAACATAGCC 
AACATAAATG ACGAGTTGCT GAAGGGTCTT GAGAAACTAG AAATTCTGGA CTTGCAGCAT 
AACAACTTAG CTCGGCTCTG GAAGCATGCC AACCCTGGCG GCCCTGTTCA GTTTCTGAAG 
GGCCTTTTTC ACCTCCACAT CCTTAACTTA GGGTCTAATG GCTTTGATGA GATCCCAGTG 
GAAGCCTTCA AGGACTTACG TGAATTGAAG AGTATTGATT TAGGAATGAA TAATTTAAAT 
ATCCTTCCAC AATCTGTCTT TGATAATCAA GTGTCACTGA AGTCATTAAG CCTTCAGAAG 
AACCTCATAA CATCTGTTCA AAAGACTGTT TTTGGGCCAG CGTTCAGGAA CCTGAGTTAT 
TTAGATATGC GTTTTAATCC ATTTGATTGT ACCTGTGAAA GCATTGCCTG GTTTGTTAAT 
TGGATTAATA GTACCCACAC CAACATCTCT GAACTGTCGA ACCATTACCT CTGCAACACT 
CCGCCCCAAT ACCATGGTTA CCCAGTAATG CTTTTCGATG TATCGCCCTG CAAAGACAGC 
GCCCCATTTG AACTCCTTTT CATGATTAAC ATCAATATCC TTTTGATTTT TATCTTTATT 
GTACTGCTCA TCCATTTTGA AGGCTGGAGG ATATCTTTTT ATTGGAATGT TTCAGTGCAT 
CGAGTTCTCG GTTTCAAAGA AATAGACAGA GCAGAGCAGT TTGAATATGC AGCATATATA 
ATTCATGCCT ATAAAGATAG GGATTGGGTC TGGAAGCACT TCTCCCCAAT GGAGGAAGAA 
GATCATACTC TCAGATTTTG TCTGGAAGAA AGGGACTTTG AGGCAGGTGT CCTTGAACTT 
GAAGCAATTG TGAACAGCAT CAGAAGGAGC AGAAAAATTA TTTTCGTTGT AACACAGAAT 
CTATTGAAAG ATCCACTATG CAAAAGATTC AAGGTTCACC ATGCAGTTCA GCAAGCTATT 
GAACAAAATC TGGATTCCAT TATATTGATC TTTCTTGAGG AGATTCCGGA TTATAAACTG 
AACCATGCAC TCTGTTTGCG AAGAGGGATG TTTAAATCTC ATTGCATCTT GAATTGGCCG 
GTTCAGAAAG AACGGGTAAA TGCTTTTCAT CATAAATTGA AAGTAGCACT TGGGTCCAGA 
AATTCAGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226014.1
CDS	547..3261
Translation	MSRPLPYHIHFFSGLLTCWILCTSSAHKCTVRHEVADCSHLKLTQIPDDLPTNITVLNLTHNQLRRLPPANFTRYSQLTILDGGFNSISKLEPELCQSLPWLEILNLQHNEISQLSDKTFIFCMNLTELHLMSNSIQKIKNDPFKNLKNLIKLDLSHNGLSSTKLGTQLQLENLQELLLSNNKISSLTPEEFDFLGNSSLKRLELSSNQIKEFSPGCFHTLGELSGLSLNNAKLSPSLTEKLCLELSNTSIENLSLSSNQLDTISHTTFDGLKQTNLTTLDLSRNSLRVMGNDSFAWLPHLEYLSLEYNNIEHLSSRSFYGLSNLRRLDLRRSFTRQSISLTSLPKIDDFSFQWLKCLEYLNMDDNNFPGIKRNTFTGLVRLKFLSLSNSFSSLRTLTNETFLSLAGCPLLLLNLTKNKISKIQSGAFSWLGHLEVLDLGLNEIGQELTGQEWRGLDNIVEIYLSYNKYLELTTNSFTSVPSLQRLMLRRVALKNVDCSPSPFRPLPNLVILDLSNNNIANINDELLKGLEKLEILDLQHNNLARLWKHANPGGPVQFLKGLFHLHILNLGSNGFDEIPVEAFKDLRELKSIDLGMNNLNILPQSVFDNQVSLKSLSLQKNLITSVQKTVFGPAFRNLSYLDMRFNPFDCTCESIAWFVNWINSTHTNISELSNHYLCNTPPQYHGYPVMLFDVSPCKDSAPFELLFMININILLIFIFIVLLIHFEGWRISFYWNVSVHRVLGFKEIDRAEQFEYAAYIIHAYKDRDWVWKHFSPMEEEDHTLRFCLEERDFEAGVLELEAIVNSIRRSRKIIFVVTQNLLKDPLCKRFKVHHAVQQAIEQNLDSIILIFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKERVNAFHHKLKVALGSRNSAH

Target ORF information:

RefSeq Version	XM_005225957.3
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 3 (TLR3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225957.3

ORF Insert Sequence:

ATGAGCAGAC CTTTGCCTTA TCATATCCAC TTCTTTTCGG GACTGTTGAC CTGTTGGATA 
CTGTGTACAT CTTCTGCCCA CAAATGTACT GTTAGACATG AAGTAGCTGA TTGCAGTCAT 
CTGAAGTTGA CGCAAATACC TGATGACCTC CCAACAAACA TAACTGTGTT GAATCTCACC 
CATAATCAAC TCAGAAGATT GCCACCTGCC AATTTTACGA GATACAGCCA ACTTACTATC 
TTGGATGGGG GATTTAACTC CATCTCAAAG CTGGAGCCAG AACTGTGTCA AAGTCTCCCT 
TGGTTGGAAA TTTTGAACCT CCAACACAAT GAGATATCTC AGCTTTCTGA TAAAACCTTT 
ATCTTCTGCA TGAATTTGAC TGAACTCCAT CTAATGTCCA ACTCAATCCA AAAAATTAAA 
AACGATCCCT TTAAAAACCT GAAGAATTTA ATCAAATTAG ACCTCTCTCA TAATGGCTTA 
TCATCTACTA AATTAGGAAC TCAGCTCCAA CTGGAGAACC TCCAAGAGCT TCTATTATCA 
AATAATAAAA TTTCTTCACT AACACCAGAA GAATTTGATT TCCTTGGCAA TTCTTCTTTA 
AAAAGATTAG AGTTGTCATC AAATCAAATC AAAGAGTTCT CTCCTGGGTG TTTTCATACC 
CTTGGAGAAT TATCTGGCCT CTCTCTGAAC AATGCCAAGC TGAGCCCCAG TCTCACAGAG 
AAGCTCTGCT TGGAACTGTC AAACACAAGC ATTGAGAATC TGTCCCTGAG CAGCAACCAG 
CTGGACACAA TCAGCCACAC GACCTTCGAT GGACTGAAGC AGACGAATCT CACCACGCTG 
GACCTTTCCC GTAACTCCTT ACGTGTGATG GGTAATGACT CCTTTGCCTG GCTTCCACAT 
CTCGAATACC TCTCTCTGGA GTATAATAAC ATAGAGCATT TGTCTTCTCG CTCTTTTTAT 
GGGCTTTCCA ACTTGAGACG CCTGGACTTG AGACGGTCTT TTACTAGACA AAGCATTTCA 
CTGACTTCGC TCCCCAAGAT TGACGATTTT TCCTTTCAGT GGCTAAAATG TTTGGAGTAC 
CTCAACATGG ACGATAACAA CTTTCCAGGC ATAAAAAGAA ATACTTTCAC GGGATTGGTG 
AGGCTGAAAT TTTTAAGTCT CTCCAACTCC TTCTCAAGTT TGCGGACTTT AACAAATGAG 
ACATTTCTAT CGCTCGCTGG TTGTCCTCTG CTCCTACTCA ACCTAACCAA AAATAAAATC 
TCAAAAATTC AGAGTGGTGC TTTTTCTTGG TTGGGGCACC TGGAGGTCCT TGACCTCGGC 
CTTAATGAGA TTGGGCAAGA ACTCACAGGC CAGGAATGGA GAGGCCTAGA CAATATTGTC 
GAAATCTACC TTTCCTACAA CAAATACCTA GAGCTGACCA CCAACTCTTT CACCTCAGTT 
CCAAGCCTTC AAAGACTGAT GCTCCGAAGG GTGGCCCTGA AAAATGTGGA CTGCTCCCCT 
TCGCCTTTTC GCCCTCTTCC GAACCTGGTC ATTCTGGATC TAAGCAACAA CAACATAGCC 
AACATAAATG ACGAGTTGCT GAAGGGTCTT GAGAAACTAG AAATTCTGGA CTTGCAGCAT 
AACAACTTAG CTCGGCTCTG GAAGCATGCC AACCCTGGCG GCCCTGTTCA GTTTCTGAAG 
GGCCTTTTTC ACCTCCACAT CCTTAACTTA GGGTCTAATG GCTTTGATGA GATCCCAGTG 
GAAGCCTTCA AGGACTTACG TGAATTGAAG AGTATTGATT TAGGAATGAA TAATTTAAAT 
ATCCTTCCAC AATCTGTCTT TGATAATCAA GTGTCACTGA AGTCATTAAG CCTTCAGAAG 
AACCTCATAA CATCTGTTCA AAAGACTGTT TTTGGGCCAG CGTTCAGGAA CCTGAGTTAT 
TTAGATATGC GTTTTAATCC ATTTGATTGT ACCTGTGAAA GCATTGCCTG GTTTGTTAAT 
TGGATTAATA GTACCCACAC CAACATCTCT GAACTGTCGA ACCATTACCT CTGCAACACT 
CCGCCCCAAT ACCATGGTTA CCCAGTAATG CTTTTCGATG TATCGCCCTG CAAAGACAGC 
GCCCCATTTG AACTCCTTTT CATGATTAAC ATCAATATCC TTTTGATTTT TATCTTTATT 
GTACTGCTCA TCCATTTTGA AGGCTGGAGG ATATCTTTTT ATTGGAATGT TTCAGTGCAT 
CGAGTTCTCG GTTTCAAAGA AATAGACAGA GCAGAGCAGT TTGAATATGC AGCATATATA 
ATTCATGCCT ATAAAGATAG GGATTGGGTC TGGAAGCACT TCTCCCCAAT GGAGGAAGAA 
GATCATACTC TCAGATTTTG TCTGGAAGAA AGGGACTTTG AGGCAGGTGT CCTTGAACTT 
GAAGCAATTG TGAACAGCAT CAGAAGGAGC AGAAAAATTA TTTTCGTTGT AACACAGAAT 
CTATTGAAAG ATCCACTATG CAAAAGATTC AAGGTTCACC ATGCAGTTCA GCAAGCTATT 
GAACAAAATC TGGATTCCAT TATATTGATC TTTCTTGAGG AGATTCCGGA TTATAAACTG 
AACCATGCAC TCTGTTTGCG AAGAGGGATG TTTAAATCTC ATTGCATCTT GAATTGGCCG 
GTTCAGAAAG AACGGGTAAA TGCTTTTCAT CATAAATTGA AAGTAGCACT TGGGTCCAGA 
AATTCAGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR3 ( XM_010820244.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818546.1 Bos taurus toll like receptor 3 (TLR3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa33896
Clone ID Related Accession (Same CDS sequence)	XM_005225957.3 , XM_010820244.2
Accession Version	XM_010820244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	toll-like receptor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104605.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGAC CTTTGCCTTA TCATATCCAC TTCTTTTCGG GACTGTTGAC CTGTTGGATA 
CTGTGTACAT CTTCTGCCCA CAAATGTACT GTTAGACATG AAGTAGCTGA TTGCAGTCAT 
CTGAAGTTGA CGCAAATACC TGATGACCTC CCAACAAACA TAACTGTGTT GAATCTCACC 
CATAATCAAC TCAGAAGATT GCCACCTGCC AATTTTACGA GATACAGCCA ACTTACTATC 
TTGGATGGGG GATTTAACTC CATCTCAAAG CTGGAGCCAG AACTGTGTCA AAGTCTCCCT 
TGGTTGGAAA TTTTGAACCT CCAACACAAT GAGATATCTC AGCTTTCTGA TAAAACCTTT 
ATCTTCTGCA TGAATTTGAC TGAACTCCAT CTAATGTCCA ACTCAATCCA AAAAATTAAA 
AACGATCCCT TTAAAAACCT GAAGAATTTA ATCAAATTAG ACCTCTCTCA TAATGGCTTA 
TCATCTACTA AATTAGGAAC TCAGCTCCAA CTGGAGAACC TCCAAGAGCT TCTATTATCA 
AATAATAAAA TTTCTTCACT AACACCAGAA GAATTTGATT TCCTTGGCAA TTCTTCTTTA 
AAAAGATTAG AGTTGTCATC AAATCAAATC AAAGAGTTCT CTCCTGGGTG TTTTCATACC 
CTTGGAGAAT TATCTGGCCT CTCTCTGAAC AATGCCAAGC TGAGCCCCAG TCTCACAGAG 
AAGCTCTGCT TGGAACTGTC AAACACAAGC ATTGAGAATC TGTCCCTGAG CAGCAACCAG 
CTGGACACAA TCAGCCACAC GACCTTCGAT GGACTGAAGC AGACGAATCT CACCACGCTG 
GACCTTTCCC GTAACTCCTT ACGTGTGATG GGTAATGACT CCTTTGCCTG GCTTCCACAT 
CTCGAATACC TCTCTCTGGA GTATAATAAC ATAGAGCATT TGTCTTCTCG CTCTTTTTAT 
GGGCTTTCCA ACTTGAGACG CCTGGACTTG AGACGGTCTT TTACTAGACA AAGCATTTCA 
CTGACTTCGC TCCCCAAGAT TGACGATTTT TCCTTTCAGT GGCTAAAATG TTTGGAGTAC 
CTCAACATGG ACGATAACAA CTTTCCAGGC ATAAAAAGAA ATACTTTCAC GGGATTGGTG 
AGGCTGAAAT TTTTAAGTCT CTCCAACTCC TTCTCAAGTT TGCGGACTTT AACAAATGAG 
ACATTTCTAT CGCTCGCTGG TTGTCCTCTG CTCCTACTCA ACCTAACCAA AAATAAAATC 
TCAAAAATTC AGAGTGGTGC TTTTTCTTGG TTGGGGCACC TGGAGGTCCT TGACCTCGGC 
CTTAATGAGA TTGGGCAAGA ACTCACAGGC CAGGAATGGA GAGGCCTAGA CAATATTGTC 
GAAATCTACC TTTCCTACAA CAAATACCTA GAGCTGACCA CCAACTCTTT CACCTCAGTT 
CCAAGCCTTC AAAGACTGAT GCTCCGAAGG GTGGCCCTGA AAAATGTGGA CTGCTCCCCT 
TCGCCTTTTC GCCCTCTTCC GAACCTGGTC ATTCTGGATC TAAGCAACAA CAACATAGCC 
AACATAAATG ACGAGTTGCT GAAGGGTCTT GAGAAACTAG AAATTCTGGA CTTGCAGCAT 
AACAACTTAG CTCGGCTCTG GAAGCATGCC AACCCTGGCG GCCCTGTTCA GTTTCTGAAG 
GGCCTTTTTC ACCTCCACAT CCTTAACTTA GGGTCTAATG GCTTTGATGA GATCCCAGTG 
GAAGCCTTCA AGGACTTACG TGAATTGAAG AGTATTGATT TAGGAATGAA TAATTTAAAT 
ATCCTTCCAC AATCTGTCTT TGATAATCAA GTGTCACTGA AGTCATTAAG CCTTCAGAAG 
AACCTCATAA CATCTGTTCA AAAGACTGTT TTTGGGCCAG CGTTCAGGAA CCTGAGTTAT 
TTAGATATGC GTTTTAATCC ATTTGATTGT ACCTGTGAAA GCATTGCCTG GTTTGTTAAT 
TGGATTAATA GTACCCACAC CAACATCTCT GAACTGTCGA ACCATTACCT CTGCAACACT 
CCGCCCCAAT ACCATGGTTA CCCAGTAATG CTTTTCGATG TATCGCCCTG CAAAGACAGC 
GCCCCATTTG AACTCCTTTT CATGATTAAC ATCAATATCC TTTTGATTTT TATCTTTATT 
GTACTGCTCA TCCATTTTGA AGGCTGGAGG ATATCTTTTT ATTGGAATGT TTCAGTGCAT 
CGAGTTCTCG GTTTCAAAGA AATAGACAGA GCAGAGCAGT TTGAATATGC AGCATATATA 
ATTCATGCCT ATAAAGATAG GGATTGGGTC TGGAAGCACT TCTCCCCAAT GGAGGAAGAA 
GATCATACTC TCAGATTTTG TCTGGAAGAA AGGGACTTTG AGGCAGGTGT CCTTGAACTT 
GAAGCAATTG TGAACAGCAT CAGAAGGAGC AGAAAAATTA TTTTCGTTGT AACACAGAAT 
CTATTGAAAG ATCCACTATG CAAAAGATTC AAGGTTCACC ATGCAGTTCA GCAAGCTATT 
GAACAAAATC TGGATTCCAT TATATTGATC TTTCTTGAGG AGATTCCGGA TTATAAACTG 
AACCATGCAC TCTGTTTGCG AAGAGGGATG TTTAAATCTC ATTGCATCTT GAATTGGCCG 
GTTCAGAAAG AACGGGTAAA TGCTTTTCAT CATAAATTGA AAGTAGCACT TGGGTCCAGA 
AATTCAGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818546.1
CDS	36..2750
Translation	MSRPLPYHIHFFSGLLTCWILCTSSAHKCTVRHEVADCSHLKLTQIPDDLPTNITVLNLTHNQLRRLPPANFTRYSQLTILDGGFNSISKLEPELCQSLPWLEILNLQHNEISQLSDKTFIFCMNLTELHLMSNSIQKIKNDPFKNLKNLIKLDLSHNGLSSTKLGTQLQLENLQELLLSNNKISSLTPEEFDFLGNSSLKRLELSSNQIKEFSPGCFHTLGELSGLSLNNAKLSPSLTEKLCLELSNTSIENLSLSSNQLDTISHTTFDGLKQTNLTTLDLSRNSLRVMGNDSFAWLPHLEYLSLEYNNIEHLSSRSFYGLSNLRRLDLRRSFTRQSISLTSLPKIDDFSFQWLKCLEYLNMDDNNFPGIKRNTFTGLVRLKFLSLSNSFSSLRTLTNETFLSLAGCPLLLLNLTKNKISKIQSGAFSWLGHLEVLDLGLNEIGQELTGQEWRGLDNIVEIYLSYNKYLELTTNSFTSVPSLQRLMLRRVALKNVDCSPSPFRPLPNLVILDLSNNNIANINDELLKGLEKLEILDLQHNNLARLWKHANPGGPVQFLKGLFHLHILNLGSNGFDEIPVEAFKDLRELKSIDLGMNNLNILPQSVFDNQVSLKSLSLQKNLITSVQKTVFGPAFRNLSYLDMRFNPFDCTCESIAWFVNWINSTHTNISELSNHYLCNTPPQYHGYPVMLFDVSPCKDSAPFELLFMININILLIFIFIVLLIHFEGWRISFYWNVSVHRVLGFKEIDRAEQFEYAAYIIHAYKDRDWVWKHFSPMEEEDHTLRFCLEERDFEAGVLELEAIVNSIRRSRKIIFVVTQNLLKDPLCKRFKVHHAVQQAIEQNLDSIILIFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKERVNAFHHKLKVALGSRNSAH

Target ORF information:

RefSeq Version	XM_010820244.2
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 3 (TLR3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820244.2

ORF Insert Sequence:

ATGAGCAGAC CTTTGCCTTA TCATATCCAC TTCTTTTCGG GACTGTTGAC CTGTTGGATA 
CTGTGTACAT CTTCTGCCCA CAAATGTACT GTTAGACATG AAGTAGCTGA TTGCAGTCAT 
CTGAAGTTGA CGCAAATACC TGATGACCTC CCAACAAACA TAACTGTGTT GAATCTCACC 
CATAATCAAC TCAGAAGATT GCCACCTGCC AATTTTACGA GATACAGCCA ACTTACTATC 
TTGGATGGGG GATTTAACTC CATCTCAAAG CTGGAGCCAG AACTGTGTCA AAGTCTCCCT 
TGGTTGGAAA TTTTGAACCT CCAACACAAT GAGATATCTC AGCTTTCTGA TAAAACCTTT 
ATCTTCTGCA TGAATTTGAC TGAACTCCAT CTAATGTCCA ACTCAATCCA AAAAATTAAA 
AACGATCCCT TTAAAAACCT GAAGAATTTA ATCAAATTAG ACCTCTCTCA TAATGGCTTA 
TCATCTACTA AATTAGGAAC TCAGCTCCAA CTGGAGAACC TCCAAGAGCT TCTATTATCA 
AATAATAAAA TTTCTTCACT AACACCAGAA GAATTTGATT TCCTTGGCAA TTCTTCTTTA 
AAAAGATTAG AGTTGTCATC AAATCAAATC AAAGAGTTCT CTCCTGGGTG TTTTCATACC 
CTTGGAGAAT TATCTGGCCT CTCTCTGAAC AATGCCAAGC TGAGCCCCAG TCTCACAGAG 
AAGCTCTGCT TGGAACTGTC AAACACAAGC ATTGAGAATC TGTCCCTGAG CAGCAACCAG 
CTGGACACAA TCAGCCACAC GACCTTCGAT GGACTGAAGC AGACGAATCT CACCACGCTG 
GACCTTTCCC GTAACTCCTT ACGTGTGATG GGTAATGACT CCTTTGCCTG GCTTCCACAT 
CTCGAATACC TCTCTCTGGA GTATAATAAC ATAGAGCATT TGTCTTCTCG CTCTTTTTAT 
GGGCTTTCCA ACTTGAGACG CCTGGACTTG AGACGGTCTT TTACTAGACA AAGCATTTCA 
CTGACTTCGC TCCCCAAGAT TGACGATTTT TCCTTTCAGT GGCTAAAATG TTTGGAGTAC 
CTCAACATGG ACGATAACAA CTTTCCAGGC ATAAAAAGAA ATACTTTCAC GGGATTGGTG 
AGGCTGAAAT TTTTAAGTCT CTCCAACTCC TTCTCAAGTT TGCGGACTTT AACAAATGAG 
ACATTTCTAT CGCTCGCTGG TTGTCCTCTG CTCCTACTCA ACCTAACCAA AAATAAAATC 
TCAAAAATTC AGAGTGGTGC TTTTTCTTGG TTGGGGCACC TGGAGGTCCT TGACCTCGGC 
CTTAATGAGA TTGGGCAAGA ACTCACAGGC CAGGAATGGA GAGGCCTAGA CAATATTGTC 
GAAATCTACC TTTCCTACAA CAAATACCTA GAGCTGACCA CCAACTCTTT CACCTCAGTT 
CCAAGCCTTC AAAGACTGAT GCTCCGAAGG GTGGCCCTGA AAAATGTGGA CTGCTCCCCT 
TCGCCTTTTC GCCCTCTTCC GAACCTGGTC ATTCTGGATC TAAGCAACAA CAACATAGCC 
AACATAAATG ACGAGTTGCT GAAGGGTCTT GAGAAACTAG AAATTCTGGA CTTGCAGCAT 
AACAACTTAG CTCGGCTCTG GAAGCATGCC AACCCTGGCG GCCCTGTTCA GTTTCTGAAG 
GGCCTTTTTC ACCTCCACAT CCTTAACTTA GGGTCTAATG GCTTTGATGA GATCCCAGTG 
GAAGCCTTCA AGGACTTACG TGAATTGAAG AGTATTGATT TAGGAATGAA TAATTTAAAT 
ATCCTTCCAC AATCTGTCTT TGATAATCAA GTGTCACTGA AGTCATTAAG CCTTCAGAAG 
AACCTCATAA CATCTGTTCA AAAGACTGTT TTTGGGCCAG CGTTCAGGAA CCTGAGTTAT 
TTAGATATGC GTTTTAATCC ATTTGATTGT ACCTGTGAAA GCATTGCCTG GTTTGTTAAT 
TGGATTAATA GTACCCACAC CAACATCTCT GAACTGTCGA ACCATTACCT CTGCAACACT 
CCGCCCCAAT ACCATGGTTA CCCAGTAATG CTTTTCGATG TATCGCCCTG CAAAGACAGC 
GCCCCATTTG AACTCCTTTT CATGATTAAC ATCAATATCC TTTTGATTTT TATCTTTATT 
GTACTGCTCA TCCATTTTGA AGGCTGGAGG ATATCTTTTT ATTGGAATGT TTCAGTGCAT 
CGAGTTCTCG GTTTCAAAGA AATAGACAGA GCAGAGCAGT TTGAATATGC AGCATATATA 
ATTCATGCCT ATAAAGATAG GGATTGGGTC TGGAAGCACT TCTCCCCAAT GGAGGAAGAA 
GATCATACTC TCAGATTTTG TCTGGAAGAA AGGGACTTTG AGGCAGGTGT CCTTGAACTT 
GAAGCAATTG TGAACAGCAT CAGAAGGAGC AGAAAAATTA TTTTCGTTGT AACACAGAAT 
CTATTGAAAG ATCCACTATG CAAAAGATTC AAGGTTCACC ATGCAGTTCA GCAAGCTATT 
GAACAAAATC TGGATTCCAT TATATTGATC TTTCTTGAGG AGATTCCGGA TTATAAACTG 
AACCATGCAC TCTGTTTGCG AAGAGGGATG TTTAAATCTC ATTGCATCTT GAATTGGCCG 
GTTCAGAAAG AACGGGTAAA TGCTTTTCAT CATAAATTGA AAGTAGCACT TGGGTCCAGA 
AATTCAGCAC ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLR3 ( XM_010820242.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818544.2 Bos taurus toll like receptor 3 (TLR3), transcript variant X1, mRNA.

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CloneID	OBa231300
Clone ID Related Accession (Same CDS sequence)	XM_010820242.3
Accession Version	XM_010820242.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	toll-like receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820242.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAAG GAACGGTCAG CTGTCGTCAC AGCAAAAGCA TGAGCAGACC TTTGCCTTAT 
CATATCCACT TCTTTTCGGG ACTGTTGACC TGTTGGATAC TGTGTACATC TTCTGCCCAC 
AAATGTACTG TTAGACATGA AGTAGCTGAT TGCAGTCATC TGAAGTTGAC GCAAATACCT 
GATGACCTCC CAACAAACAT AACTGTGTTG AATCTCACCC ATAATCAACT CAGAAGATTG 
CCACCTGCCA ATTTTACGAG ATACAGCCAA CTTACTATCT TGGATGGGGG ATTTAACTCC 
ATCTCAAAGC TGGAGCCAGA ACTGTGTCAA AGTCTCCCTT GGTTGGAAAT TTTGAACCTC 
CAACACAATG AGATATCTCA GCTTTCTGAT AAAACCTTTA TCTTCTGCAT GAATTTGACT 
GAACTCCATC TAATGTCCAA CTCAATCCAA AAAATTAAAA ACGATCCCTT TAAAAACCTG 
AAGAATTTAA TCAAATTAGA CCTCTCTCAT AATGGCTTAT CATCTACTAA ATTAGGAACT 
CAGCTCCAAC TGGAGAACCT CCAAGAGCTT CTATTATCAA ATAATAAAAT TTCTTCACTA 
ACACCAGAAG AATTTGATTT CCTTGGCAAT TCTTCTTTAA AAAGATTAGA GTTGTCATCA 
AATCAAATCA AAGAGTTCTC TCCTGGGTGT TTTCATACCC TTGGAGAATT ATCTGGCCTC 
TCTCTGAACA ATGCCAAGCT GAGCCCCAGT CTCACAGAGA AGCTCTGCTT GGAACTGTCA 
AACACAAGCA TTGAGAATCT GTCCCTGAGC AGCAACCAGC TGGACACAAT CAGCCACACG 
ACCTTCGATG GACTGAAGCA GACGAATCTC ACCACGCTGG ACCTTTCCCG TAACTCCTTA 
CGTGTGATGG GTAATGACTC CTTTGCCTGG CTTCCACATC TCGAATACCT CTCTCTGGAG 
TATAATAACA TAGAGCATTT GTCTTCTCGC TCTTTTTATG GGCTTTCCAA CTTGAGACGC 
CTGGACTTGA GACGGTCTTT TACTAGACAA AGCATTTCAC TGACTTCGCT CCCCAAGATT 
GACGATTTTT CCTTTCAGTG GCTAAAATGT TTGGAGTACC TCAACATGGA CGATAACAAC 
TTTCCAGGCA TAAAAAGAAA TACTTTCACG GGATTGGTGA GGCTGAAATT TTTAAGTCTC 
TCCAACTCCT TCTCAAGTTT GCGGACTTTA ACAAATGAGA CATTTCTATC GCTCGCTGGT 
TGTCCTCTGC TCCTACTCAA CCTAACCAAA AATAAAATCT CAAAAATTCA GAGTGGTGCT 
TTTTCTTGGT TGGGGCACCT GGAGGTCCTT GACCTCGGCC TTAATGAGAT TGGGCAAGAA 
CTCACAGGCC AGGAATGGAG AGGCCTAGAC AATATTGTCG AAATCTACCT TTCCTACAAC 
AAATACCTAG AGCTGACCAC CAACTCTTTC ACCTCAGTTC CAAGCCTTCA AAGACTGATG 
CTCCGAAGGG TGGCCCTGAA AAATGTGGAC TGCTCCCCTT CGCCTTTTCG CCCTCTTCCG 
AACCTGGTCA TTCTGGATCT AAGCAACAAC AACATAGCCA ACATAAATGA CGAGTTGCTG 
AAGGGTCTTG AGAAACTAGA AATTCTGGAC TTGCAGCATA ACAACTTAGC TCGGCTCTGG 
AAGCATGCCA ACCCTGGCGG CCCTGTTCAG TTTCTGAAGG GCCTTTTTCA CCTCCACATC 
CTTAACTTAG GGTCTAATGG CTTTGATGAG ATCCCAGTGG AAGCCTTCAA GGACTTACGT 
GAATTGAAGA GTATTGATTT AGGAATGAAT AATTTAAATA TCCTTCCACA ATCTGTCTTT 
GATAATCAAG TGTCACTGAA GTCATTAAGC CTTCAGAAGA ACCTCATAAC ATCTGTTCAA 
AAGACTGTTT TTGGGCCAGC GTTCAGGAAC CTGAGTTATT TAGATATGCG TTTTAATCCA 
TTTGATTGTA CCTGTGAAAG CATTGCCTGG TTTGTTAATT GGATTAATAG TACCCACACC 
AACATCTCTG AACTGTCGAA CCATTACCTC TGCAACACTC CGCCCCAATA CCATGGTTAC 
CCAGTAATGC TTTTCGATGT ATCACCCTGC AAAGACAGCG CCCCATTTGA ACTCCTTTTC 
ATGATTAACA TCAATATCCT TTTGATTTTT ATCTTTATTG TACTGCTCAT CCATTTTGAA 
GGCTGGAGGA TATCTTTTTA TTGGAATGTT TCAGTGCATC GAGTTCTCGG TTTCAAAGAA 
ATAGACAGAG CAGAGCAGTT TGAATATGCA GCATATATAA TTCATGCCTA TAAAGATAGG 
GATTGGGTCT GGAAGCACTT CTCCCCAATG GAGGAAGAAG ATCATACTCT CAGATTTTGT 
CTGGAAGAAA GGGACTTTGA GGCAGGTGTC CTTGAACTTG AAGCAATTGT GAACAGCATC 
AGAAGGAGCA GAAAAATTAT TTTCGTTGTA ACACAGAATC TATTGAAAGA TCCACTATGC 
AAAAGATTCA AGGTTCACCA TGCAGTTCAG CAAGCTATTG AACAAAATCT GGATTCCATT 
ATATTGATCT TTCTTGAGGA GATTCCGGAT TATAAACTGA ACCATGCACT CTGTTTGCGA 
AGAGGGATGT TTAAATCTCA TTGCATCTTG AATTGGCCGG TTCAGAAGAA CGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010818544.2
CDS	64..2760
Translation	MKKGTVSCRHSKSMSRPLPYHIHFFSGLLTCWILCTSSAHKCTVRHEVADCSHLKLTQIPDDLPTNITVLNLTHNQLRRLPPANFTRYSQLTILDGGFNSISKLEPELCQSLPWLEILNLQHNEISQLSDKTFIFCMNLTELHLMSNSIQKIKNDPFKNLKNLIKLDLSHNGLSSTKLGTQLQLENLQELLLSNNKISSLTPEEFDFLGNSSLKRLELSSNQIKEFSPGCFHTLGELSGLSLNNAKLSPSLTEKLCLELSNTSIENLSLSSNQLDTISHTTFDGLKQTNLTTLDLSRNSLRVMGNDSFAWLPHLEYLSLEYNNIEHLSSRSFYGLSNLRRLDLRRSFTRQSISLTSLPKIDDFSFQWLKCLEYLNMDDNNFPGIKRNTFTGLVRLKFLSLSNSFSSLRTLTNETFLSLAGCPLLLLNLTKNKISKIQSGAFSWLGHLEVLDLGLNEIGQELTGQEWRGLDNIVEIYLSYNKYLELTTNSFTSVPSLQRLMLRRVALKNVDCSPSPFRPLPNLVILDLSNNNIANINDELLKGLEKLEILDLQHNNLARLWKHANPGGPVQFLKGLFHLHILNLGSNGFDEIPVEAFKDLRELKSIDLGMNNLNILPQSVFDNQVSLKSLSLQKNLITSVQKTVFGPAFRNLSYLDMRFNPFDCTCESIAWFVNWINSTHTNISELSNHYLCNTPPQYHGYPVMLFDVSPCKDSAPFELLFMININILLIFIFIVLLIHFEGWRISFYWNVSVHRVLGFKEIDRAEQFEYAAYIIHAYKDRDWVWKHFSPMEEEDHTLRFCLEERDFEAGVLELEAIVNSIRRSRKIIFVVTQNLLKDPLCKRFKVHHAVQQAIEQNLDSIILIFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKNG

Target ORF information:

RefSeq Version	XM_010820242.3
Organism	Bos taurus(cattle)
Definition	Bos taurus toll like receptor 3 (TLR3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820242.3

ORF Insert Sequence:

ATGAAAAAAG GAACGGTCAG CTGTCGTCAC AGCAAAAGCA TGAGCAGACC TTTGCCTTAT 
CATATCCACT TCTTTTCGGG ACTGTTGACC TGTTGGATAC TGTGTACATC TTCTGCCCAC 
AAATGTACTG TTAGACATGA AGTAGCTGAT TGCAGTCATC TGAAGTTGAC GCAAATACCT 
GATGACCTCC CAACAAACAT AACTGTGTTG AATCTCACCC ATAATCAACT CAGAAGATTG 
CCACCTGCCA ATTTTACGAG ATACAGCCAA CTTACTATCT TGGATGGGGG ATTTAACTCC 
ATCTCAAAGC TGGAGCCAGA ACTGTGTCAA AGTCTCCCTT GGTTGGAAAT TTTGAACCTC 
CAACACAATG AGATATCTCA GCTTTCTGAT AAAACCTTTA TCTTCTGCAT GAATTTGACT 
GAACTCCATC TAATGTCCAA CTCAATCCAA AAAATTAAAA ACGATCCCTT TAAAAACCTG 
AAGAATTTAA TCAAATTAGA CCTCTCTCAT AATGGCTTAT CATCTACTAA ATTAGGAACT 
CAGCTCCAAC TGGAGAACCT CCAAGAGCTT CTATTATCAA ATAATAAAAT TTCTTCACTA 
ACACCAGAAG AATTTGATTT CCTTGGCAAT TCTTCTTTAA AAAGATTAGA GTTGTCATCA 
AATCAAATCA AAGAGTTCTC TCCTGGGTGT TTTCATACCC TTGGAGAATT ATCTGGCCTC 
TCTCTGAACA ATGCCAAGCT GAGCCCCAGT CTCACAGAGA AGCTCTGCTT GGAACTGTCA 
AACACAAGCA TTGAGAATCT GTCCCTGAGC AGCAACCAGC TGGACACAAT CAGCCACACG 
ACCTTCGATG GACTGAAGCA GACGAATCTC ACCACGCTGG ACCTTTCCCG TAACTCCTTA 
CGTGTGATGG GTAATGACTC CTTTGCCTGG CTTCCACATC TCGAATACCT CTCTCTGGAG 
TATAATAACA TAGAGCATTT GTCTTCTCGC TCTTTTTATG GGCTTTCCAA CTTGAGACGC 
CTGGACTTGA GACGGTCTTT TACTAGACAA AGCATTTCAC TGACTTCGCT CCCCAAGATT 
GACGATTTTT CCTTTCAGTG GCTAAAATGT TTGGAGTACC TCAACATGGA CGATAACAAC 
TTTCCAGGCA TAAAAAGAAA TACTTTCACG GGATTGGTGA GGCTGAAATT TTTAAGTCTC 
TCCAACTCCT TCTCAAGTTT GCGGACTTTA ACAAATGAGA CATTTCTATC GCTCGCTGGT 
TGTCCTCTGC TCCTACTCAA CCTAACCAAA AATAAAATCT CAAAAATTCA GAGTGGTGCT 
TTTTCTTGGT TGGGGCACCT GGAGGTCCTT GACCTCGGCC TTAATGAGAT TGGGCAAGAA 
CTCACAGGCC AGGAATGGAG AGGCCTAGAC AATATTGTCG AAATCTACCT TTCCTACAAC 
AAATACCTAG AGCTGACCAC CAACTCTTTC ACCTCAGTTC CAAGCCTTCA AAGACTGATG 
CTCCGAAGGG TGGCCCTGAA AAATGTGGAC TGCTCCCCTT CGCCTTTTCG CCCTCTTCCG 
AACCTGGTCA TTCTGGATCT AAGCAACAAC AACATAGCCA ACATAAATGA CGAGTTGCTG 
AAGGGTCTTG AGAAACTAGA AATTCTGGAC TTGCAGCATA ACAACTTAGC TCGGCTCTGG 
AAGCATGCCA ACCCTGGCGG CCCTGTTCAG TTTCTGAAGG GCCTTTTTCA CCTCCACATC 
CTTAACTTAG GGTCTAATGG CTTTGATGAG ATCCCAGTGG AAGCCTTCAA GGACTTACGT 
GAATTGAAGA GTATTGATTT AGGAATGAAT AATTTAAATA TCCTTCCACA ATCTGTCTTT 
GATAATCAAG TGTCACTGAA GTCATTAAGC CTTCAGAAGA ACCTCATAAC ATCTGTTCAA 
AAGACTGTTT TTGGGCCAGC GTTCAGGAAC CTGAGTTATT TAGATATGCG TTTTAATCCA 
TTTGATTGTA CCTGTGAAAG CATTGCCTGG TTTGTTAATT GGATTAATAG TACCCACACC 
AACATCTCTG AACTGTCGAA CCATTACCTC TGCAACACTC CGCCCCAATA CCATGGTTAC 
CCAGTAATGC TTTTCGATGT ATCACCCTGC AAAGACAGCG CCCCATTTGA ACTCCTTTTC 
ATGATTAACA TCAATATCCT TTTGATTTTT ATCTTTATTG TACTGCTCAT CCATTTTGAA 
GGCTGGAGGA TATCTTTTTA TTGGAATGTT TCAGTGCATC GAGTTCTCGG TTTCAAAGAA 
ATAGACAGAG CAGAGCAGTT TGAATATGCA GCATATATAA TTCATGCCTA TAAAGATAGG 
GATTGGGTCT GGAAGCACTT CTCCCCAATG GAGGAAGAAG ATCATACTCT CAGATTTTGT 
CTGGAAGAAA GGGACTTTGA GGCAGGTGTC CTTGAACTTG AAGCAATTGT GAACAGCATC 
AGAAGGAGCA GAAAAATTAT TTTCGTTGTA ACACAGAATC TATTGAAAGA TCCACTATGC 
AAAAGATTCA AGGTTCACCA TGCAGTTCAG CAAGCTATTG AACAAAATCT GGATTCCATT 
ATATTGATCT TTCTTGAGGA GATTCCGGAT TATAAACTGA ACCATGCACT CTGTTTGCGA 
AGAGGGATGT TTAAATCTCA TTGCATCTTG AATTGGCCGG TTCAGAAGAA CGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Toll-like receptors 3, 7 and 8 are upregulated in the placental caruncle and fetal spleen of Neospora caninum experimentally infected cattle.
Veterinary parasitology23658-61(2017 Mar)
Marin MS,Hecker YP,Quintana S,P?rez SE,Leunda MR,Cant?n GJ,Cobo ER,Moore DP,Ode?n AC

Involvement of toll-like receptors 3 and 7/8 in the neuropathogenesis of bovine herpesvirus types 1 and 5.
Research in veterinary science1071-7(2016 Aug)
Rensetti D,Marin M,Quintana S,Mor?n P,Verna A,Ode?n A,P?rez S

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Detection of polymorphisms in bovine toll-like receptors 3, 7, 8, and 9.
Genomics89(6)745-55(2007 Jun)
Cargill EJ,Womack JE

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TLR3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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