THBS1 cDNA ORF clone, Bos taurus(cattle)

The following THBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa45161 XM_024997243.1
Latest version!
Bos taurus thrombospondin 1 (THBS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa43367 NM_174196.1
Latest version!
Bos taurus thrombospondin 1 (THBS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa45161
    Clone ID Related Accession (Same CDS sequence) XM_024997243.1
    Accession Version XM_024997243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product thrombospondin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTGTTGC ATGCCTGCGG CTCCAACCGC 
    ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC
    CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGCCCTGACC CTTCTAGCCC AGCTTTCCGC
    ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT
    GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC
    CGGGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGATC
    TCCAATGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCATGTG
    GTGTCGGTGG AAGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTGTTTGTG
    CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT
    GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGGCT CCGCATTGCC
    AAAGGAGGTG TCAACGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA
    ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTGTC
    ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATTGGC
    CACAAGACAA AGGACCTGCA AGCCATCTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG
    GTCCTGGAGC TCAGGGGTCT ACGCACCATC GTGACCACGC TGCAGGACAG TATCCGCAAA
    GTGACCGAAG AGAACAAAGA GCTGGCCAAC GAGCTGAGGA GGCCCCCACT CTGCTACCAC
    AACGGAGTGC AGTACAGGAC TGGCGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC
    TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAT
    GCCACAGTTC CGGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC
    GACGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC
    CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG
    ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG CGGCTGGAGC
    CACTGGTCCC CATGGTCATC TTGCTCCGTA ACATGTGGAG ACGGTGTGAT CACAAGGATC
    CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG
    GAGACCAAAG CCTGCCAGAA AGACTCCTGC CCCATCAATG GAGGCTGGGG ACCTTGGTCA
    CCATGGGACA TCTGTTCTGT CACCTGTGGA GGAGGGGTAC AGAAACGTAG CCGGCTCTGC
    AACAACCCCA CACCCCAGTT TGGAGGCAAG GACTGCATTG GTGATGTGAC AGAAAACCAG
    ATCTGCAACA AGCAGGACTG TCCCATTGAC GGATGCCTGT CCAATCCCTG CTTTGCTGGT
    GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCCTGTCC CCCAGGCTAT
    AGTGGAGATG GAGTCGAGTG CAAAGACGTT GATGAGTGCA AAGAAGTCCC TGATGCCTGC
    TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC
    TGCCCACCGC GCTTCACTGG CTCGCAGCCC TTTGGCCGGG GCGTGGAACA TGCCACCGCC
    AACAAGCAGG TATGCAAGCC CCGAAACCCC TGCACAGACG GGACACACGA CTGCAACAAG
    AACGCCAAGT GCAACTACCT GGGCCACTAC AGCGACCCCA TGTACCGCTG CGAGTGCAAG
    CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC
    AATGAGGACC TGCTGTGCGT GGCCAACGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC
    AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGCGAT
    GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC
    CCAGCCCAGT ACGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC
    AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGTGCAGCT
    GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG
    GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGATCAGT GTGACAACTG CCCCCTGGAA
    CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT
    CAGGATATTG ATGAAGACGG CCACCAGAAC AATCTGGACA ACTGTCCCTA CGTGCCCAAC
    GCCAACCAGG CTGACCATGA CAAGGATGGC AAAGGCGATG CCTGTGACCA TGATGACGAC
    AATGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG
    GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG
    CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA
    TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA
    CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT
    TATGACGAAT TTAACGCCGT GGACTTCAGT GGCACCTTCT TCATCAACAC CGAGAGGGAT
    GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG
    TGGAAGCAAG TCACTCAGTC CTACTGGGAC ACCAACCCCA CGAGGGCTCA GGGGTACTCT
    GGACTTTCCG TGAAGGTTGT AAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC
    CTGTGGCACA CAGGAAACAC CTCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC
    ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCATCTGA GCCACAGGCC AAAGACAGGT
    TTCATCAGAG TGGTAATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT
    GACAAAACCT ATGCTGGTGG GAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC
    TTCTCCGACC TGAAATATGA ATGCAGAGAC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853011.1
    CDS195..3710
    Translation

    Target ORF information:

    RefSeq Version XM_024997243.1
    Organism Bos taurus(cattle)
    Definition Bos taurus thrombospondin 1 (THBS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTGTTGC ATGCCTGCGG CTCCAACCGC 
    ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC
    CGCAAGGGCT CTGGGCGCCG ACTGGTGAAG GGCCCTGACC CTTCTAGCCC AGCTTTCCGC
    ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT
    GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC
    CGGGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGATC
    TCCAATGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCATGTG
    GTGTCGGTGG AAGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTGTTTGTG
    CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT
    GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGGCT CCGCATTGCC
    AAAGGAGGTG TCAACGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA
    ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTGTC
    ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATTGGC
    CACAAGACAA AGGACCTGCA AGCCATCTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG
    GTCCTGGAGC TCAGGGGTCT ACGCACCATC GTGACCACGC TGCAGGACAG TATCCGCAAA
    GTGACCGAAG AGAACAAAGA GCTGGCCAAC GAGCTGAGGA GGCCCCCACT CTGCTACCAC
    AACGGAGTGC AGTACAGGAC TGGCGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC
    TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAT
    GCCACAGTTC CGGATGGAGA ATGCTGCCCA CGGTGCTGGC CAGCCAGCGA CTCTGCAGAC
    GACGGCTGGT CCCCGTGGTC TGAGTGGACC TCTTGCTCTG TGACCTGTGG CAATGGAATC
    CAGCAGCGTG GCCGCTCCTG CGACAGCCTC AACAACAGAT GCGAGGGCTC CTCTGTGCAG
    ACGCGGACCT GCCACATCCA GGAGTGTGAC AAGAGATTTA AACAGGATGG CGGCTGGAGC
    CACTGGTCCC CATGGTCATC TTGCTCCGTA ACATGTGGAG ACGGTGTGAT CACAAGGATC
    CGGCTCTGCA ACTCCCCCAG CCCCCAGATG AATGGGAAGC CATGTGAGGG CAAAGCCCGG
    GAGACCAAAG CCTGCCAGAA AGACTCCTGC CCCATCAATG GAGGCTGGGG ACCTTGGTCA
    CCATGGGACA TCTGTTCTGT CACCTGTGGA GGAGGGGTAC AGAAACGTAG CCGGCTCTGC
    AACAACCCCA CACCCCAGTT TGGAGGCAAG GACTGCATTG GTGATGTGAC AGAAAACCAG
    ATCTGCAACA AGCAGGACTG TCCCATTGAC GGATGCCTGT CCAATCCCTG CTTTGCTGGT
    GTCCAGTGTA CCAGCTACCC TGATGGCAGC TGGAAGTGTG GTGCCTGTCC CCCAGGCTAT
    AGTGGAGATG GAGTCGAGTG CAAAGACGTT GATGAGTGCA AAGAAGTCCC TGATGCCTGC
    TTCAACCACA ATGGAGAGCA CAGGTGTGAG AACACAGACC CCGGCTACAA CTGCCTGCCC
    TGCCCACCGC GCTTCACTGG CTCGCAGCCC TTTGGCCGGG GCGTGGAACA TGCCACCGCC
    AACAAGCAGG TATGCAAGCC CCGAAACCCC TGCACAGACG GGACACACGA CTGCAACAAG
    AACGCCAAGT GCAACTACCT GGGCCACTAC AGCGACCCCA TGTACCGCTG CGAGTGCAAG
    CCTGGCTACG CCGGCAACGG CATCATCTGC GGGGAGGACA CAGACCTGGA CGGCTGGCCC
    AATGAGGACC TGCTGTGCGT GGCCAACGCA ACTTACCACT GCAGAAAGGA TAATTGCCCC
    AACCTTCCCA ACTCAGGGCA GGAAGACTAT GACAAGGATG GAATCGGCGA TGCCTGCGAT
    GATGACGATG ACAATGATAA GATTCCAGAT GACAGGGACA ACTGTCCATT CCATTACAAC
    CCAGCCCAGT ACGACTATGA CAGAGATGAC GTGGGAGACC GCTGTGACAA CTGCCCCTAC
    AACCACAACC CAGACCAGGC TGACACAGAT AACAATGGGG AAGGAGACGC CTGTGCAGCT
    GACATTGATG GGGACGGTAT CCTCAATGAA CGGGACAACT GCCAGTATGT CTACAATGTG
    GACCAGAAAG ACACTGACAT GGACGGGGTT GGTGATCAGT GTGACAACTG CCCCCTGGAA
    CACAATCCAG ACCAGCTCGA CTCTGACTCG GACCGCATTG GAGACACCTG TGACAACAAT
    CAGGATATTG ATGAAGACGG CCACCAGAAC AATCTGGACA ACTGTCCCTA CGTGCCCAAC
    GCCAACCAGG CTGACCATGA CAAGGATGGC AAAGGCGATG CCTGTGACCA TGATGACGAC
    AATGATGGCA TTCCTGATGA CCGGGACAAC TGCAGGCTGG TGCCCAATCC TGACCAGAAG
    GACTCTGATG GTGATGGTCG AGGTGATGCT TGCAAAGATG ATTTTGACCA GGACAAGGTG
    CCAGACATTG ATGACATCTG TCCCGAAAAT GTTGATATCA GTGAGACTGA TTTCCGCCGA
    TTCCAGATGA TTCCTCTAGA TCCCAAAGGG ACATCCCAGA ATGACCCTAA CTGGGTTGTA
    CGCCATCAGG GTAAAGAACT CGTCCAGACT GTCAACTGTG ACCCTGGACT TGCTGTAGGT
    TATGACGAAT TTAACGCCGT GGACTTCAGT GGCACCTTCT TCATCAACAC CGAGAGGGAT
    GACGACTATG CCGGCTTTGT GTTTGGCTAC CAGTCCAGCA GCCGCTTCTA TGTTGTGATG
    TGGAAGCAAG TCACTCAGTC CTACTGGGAC ACCAACCCCA CGAGGGCTCA GGGGTACTCT
    GGACTTTCCG TGAAGGTTGT AAACTCCACC ACGGGGCCTG GCGAGCACCT GCGGAATGCC
    CTGTGGCACA CAGGAAACAC CTCTGGCCAG GTGCGCACAC TGTGGCATGA CCCTCGTCAC
    ATTGGCTGGA AAGATTTCAC TGCCTACAGA TGGCATCTGA GCCACAGGCC AAAGACAGGT
    TTCATCAGAG TGGTAATGTA TGAAGGGAAG AAAATCATGG CTGACTCAGG ACCCATCTAT
    GACAAAACCT ATGCTGGTGG GAGGCTAGGC TTGTTCGTCT TCTCTCAAGA AATGGTGTTC
    TTCTCCGACC TGAAATATGA ATGCAGAGAC TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa43367
    Clone ID Related Accession (Same CDS sequence) NM_174196.1
    Accession Version NM_174196.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-10
    Organism Bos taurus(cattle)
    Product thrombospondin-1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB005287.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB005287.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTGTTGC ATGCCTGCGG CTCCAACCGC 
    ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC
    CGCAAGCGGT CTGGGCGCCG ACTGGTGAAG GGCCCTGACC CTTCTAGCCC AGCTTTCCGC
    ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT
    GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC
    CGGGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGATC
    TCCAATGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCATGTG
    GTGTCGGTGG AAGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTGTTTGTG
    CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT
    GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGGCT CCGCATTGCC
    AAAGGAGGTG TCAACGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA
    ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTGTC
    ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATTGGC
    CACAAGACAA AGGACCTGCA AGCCATCTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG
    GTCCTGGAGC TCAGGGGTCT ACGCACCATC GTGACCACGC TGCAGGACAG TATCCGCAAA
    GTGACCGAAG AGAACAAAGA GCTGGCCAAC GAGCTGAGGA GGCCCCCACT CTGCTACCAC
    AACGGAGTGC AGTACAGGAC TGGCGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC
    TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAT
    GCCACAGTTC CGGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT
    GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG
    CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG
    CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGCGG CTGGAGCCAC
    TGGTCCCCAT GGTCATCTTG CTCCGTAACA TGTGGAGACG GTGTGATCAC AAGGATCCGG
    CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG
    ACCAAAGCCT GCCAGAAAGA CTCCTGCCCC ATCAATGGAG GCTGGGGACC TTGGTCACCA
    TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGCCG GCTCTGCAAC
    AACCCCAAAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTGACAGA AAACCAGATC
    TGCAACAAGC AGGACTGTCC CATTGACGGA TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC
    CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CCTGTCCCCC AGGCTATAGT
    GGAGATGGAG TCGAGTGCAA AGACGTTGAT GAGTGCAAAG AAGTCCCTGA TGCCTGCTTC
    AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC
    CCACCGCGCT TCACTGGCTC GCAGCCCTTT GGCCGGGGCG TGGAACATGC CACCGCCAAC
    AAGCAGGTAT GCAAGCCCCG AAACCCCTGC ACAGACGGGA CACACGACTG CAACAAGAAC
    GCCAAGTGCA ACTACCTGGG CCACTACAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT
    GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAT
    GAGGACCTGC TGTGCGTGGC CAACGCAACT TACCACTGCA GAAAGGATAA TTGCCCCAAC
    CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGCGATGAT
    GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA
    GCCCAGTACG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC
    CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG TGCAGCTGAC
    ATTGATGGGG ACAGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC
    CAGAAAGACA CTGACATGGA CGGGGTTGGT GATCAGTGTG ACAACTGCCC CCTGGAACAC
    AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG
    GATATTGATG AAGACGGCCA CCAGAACAAT CTGGACAACT GTCCCTACGT GCCCAACGCC
    AACCAGGCTG ACCATGACAA GGATGGCAAA GGCGATGCCT GTGACCATGA TGACGACAAT
    GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC
    TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA
    GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC
    CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC
    CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT
    GACGAATTTA ACGCCGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA GAGGGATGAC
    GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG
    AAGCAAGTCA CTCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG GTACTCTGGA
    CTTTCCGTGA AGGTTGTAAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG
    TGGCACACAG GAAACACCTC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT
    GGCTGGAAAG ATTTCACTGC CTACAGATGG CATCTGAGCC ACAGGCCAAA GACAGGTTTC
    ATCAGAGTGG TAATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC
    AAAACCTATG CTGGTGGGAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC
    TCCGACCTGA AATATGAATG CAGAGACTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776621.1
    CDS148..3660
    Translation

    Target ORF information:

    RefSeq Version NM_174196.1
    Organism Bos taurus(cattle)
    Definition Bos taurus thrombospondin 1 (THBS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGCTGG CCTGGGGACT CGGTGTCCTG CTCCTGTTGC ATGCCTGCGG CTCCAACCGC 
    ATTCCAGAGT CTGGGGGAGA CAACAGTGTG TTTGACATCT TTGAACTCAC CGGAGCTGCC
    CGCAAGCGGT CTGGGCGCCG ACTGGTGAAG GGCCCTGACC CTTCTAGCCC AGCTTTCCGC
    ATCGAGGATG CCAACCTGAT CCCCCCTGTG CCTGACAAGA AGTTCCAAGA CCTAGTGGAT
    GCTGTGCGGG CGGAGAAAGG TTTCCTCCTC CTGGCCTCCC TGAGGCAAAT GAAGAAGACC
    CGGGGTACCC TGCTGGCTGT GGAGCGGAAA GACCACTCTG GCCAGGTCTT CAGCGTGATC
    TCCAATGGCA AGGCGGGCAC CCTGGACCTG AGCCTGACCG TGCAGGGGAA GCAGCATGTG
    GTGTCGGTGG AAGAAGCACT CCTGGCGACT GGCCAGTGGA AGAGCATCAC CCTGTTTGTG
    CAGGAGGACA GGGCCCAGCT GTACATCGAC TGTGAGAAGA TGGAGAATGC GGAGCTGGAT
    GTCCCCATCC AGAGCATCTT CACCAGGGAC CTGGCCAGCA TCGCCAGGCT CCGCATTGCC
    AAAGGAGGTG TCAACGACAA TTTCCAGGGG GTGCTGCAGA ATGTAAGGTT TGTCTTTGGA
    ACCACACCAG AAGACATCCT CAGGAACAAA GGCTGCTCCA GCTCTACCAG TGTCTTTGTC
    ACCCTTGACA ACAACGTGGT GAATGGGTCC AGCCCTGCCA TCCGCACCGA CTACATTGGC
    CACAAGACAA AGGACCTGCA AGCCATCTGT GGCATCTCAT GTGACGAGCT GTCCAGCATG
    GTCCTGGAGC TCAGGGGTCT ACGCACCATC GTGACCACGC TGCAGGACAG TATCCGCAAA
    GTGACCGAAG AGAACAAAGA GCTGGCCAAC GAGCTGAGGA GGCCCCCACT CTGCTACCAC
    AACGGAGTGC AGTACAGGAC TGGCGACGAG TGGACGGTGG ACAGCTGCAC TGAGTGTCGC
    TGCCAGAACT CAGTTACCAT CTGCAAAAAA GTGTCCTGTC CCATCATGCC CTGCTCCAAT
    GCCACAGTTC CGGATGGAGA ATGCTGCCCA CGGTGCTGGC CCAGCGACTC TGCAGACGAT
    GGCTGGTCCC CGTGGTCTGA GTGGACCTCT TGCTCTGTGA CCTGTGGCAA TGGAATCCAG
    CAGCGTGGCC GCTCCTGCGA CAGCCTCAAC AACAGATGCG AGGGCTCCTC TGTGCAGACG
    CGGACCTGCC ACATCCAGGA GTGTGACAAG AGATTTAAAC AGGATGGCGG CTGGAGCCAC
    TGGTCCCCAT GGTCATCTTG CTCCGTAACA TGTGGAGACG GTGTGATCAC AAGGATCCGG
    CTCTGCAACT CCCCCAGCCC CCAGATGAAT GGGAAGCCAT GTGAGGGCAA AGCCCGGGAG
    ACCAAAGCCT GCCAGAAAGA CTCCTGCCCC ATCAATGGAG GCTGGGGACC TTGGTCACCA
    TGGGACATCT GTTCTGTCAC CTGTGGAGGA GGGGTACAGA AACGTAGCCG GCTCTGCAAC
    AACCCCAAAC CCCAGTTTGG AGGCAAGGAC TGCGTTGGTG ATGTGACAGA AAACCAGATC
    TGCAACAAGC AGGACTGTCC CATTGACGGA TGCCTGTCCA ATCCCTGCTT TGCTGGTGTC
    CAGTGTACCA GCTACCCTGA TGGCAGCTGG AAGTGTGGTG CCTGTCCCCC AGGCTATAGT
    GGAGATGGAG TCGAGTGCAA AGACGTTGAT GAGTGCAAAG AAGTCCCTGA TGCCTGCTTC
    AACCACAATG GAGAGCACAG GTGTGAGAAC ACAGACCCCG GCTACAACTG CCTGCCCTGC
    CCACCGCGCT TCACTGGCTC GCAGCCCTTT GGCCGGGGCG TGGAACATGC CACCGCCAAC
    AAGCAGGTAT GCAAGCCCCG AAACCCCTGC ACAGACGGGA CACACGACTG CAACAAGAAC
    GCCAAGTGCA ACTACCTGGG CCACTACAGC GACCCCATGT ACCGCTGCGA GTGCAAGCCT
    GGCTACGCCG GCAACGGCAT CATCTGCGGG GAGGACACAG ACCTGGACGG CTGGCCCAAT
    GAGGACCTGC TGTGCGTGGC CAACGCAACT TACCACTGCA GAAAGGATAA TTGCCCCAAC
    CTTCCCAACT CAGGGCAGGA AGACTATGAC AAGGATGGAA TCGGCGATGC CTGCGATGAT
    GACGATGACA ATGATAAGAT TCCAGATGAC AGGGACAACT GTCCATTCCA TTACAACCCA
    GCCCAGTACG ACTATGACAG AGATGACGTG GGAGACCGCT GTGACAACTG CCCCTACAAC
    CACAACCCAG ACCAGGCTGA CACAGATAAC AATGGGGAAG GAGACGCCTG TGCAGCTGAC
    ATTGATGGGG ACAGTATCCT CAATGAACGG GACAACTGCC AGTATGTCTA CAATGTGGAC
    CAGAAAGACA CTGACATGGA CGGGGTTGGT GATCAGTGTG ACAACTGCCC CCTGGAACAC
    AATCCAGACC AGCTCGACTC TGACTCGGAC CGCATTGGAG ACACCTGTGA CAACAATCAG
    GATATTGATG AAGACGGCCA CCAGAACAAT CTGGACAACT GTCCCTACGT GCCCAACGCC
    AACCAGGCTG ACCATGACAA GGATGGCAAA GGCGATGCCT GTGACCATGA TGACGACAAT
    GATGGCATTC CTGATGACCG GGACAACTGC AGGCTGGTGC CCAATCCTGA CCAGAAGGAC
    TCTGATGGTG ATGGTCGAGG TGATGCTTGC AAAGATGATT TTGACCAGGA CAAGGTGCCA
    GACATTGATG ACATCTGTCC CGAAAATGTT GATATCAGTG AGACTGATTT CCGCCGATTC
    CAGATGATTC CTCTAGATCC CAAAGGGACA TCCCAGAATG ACCCTAACTG GGTTGTACGC
    CATCAGGGTA AAGAACTCGT CCAGACTGTC AACTGTGACC CTGGACTTGC TGTAGGTTAT
    GACGAATTTA ACGCCGTGGA CTTCAGTGGC ACCTTCTTCA TCAACACCGA GAGGGATGAC
    GACTATGCCG GCTTTGTGTT TGGCTACCAG TCCAGCAGCC GCTTCTATGT TGTGATGTGG
    AAGCAAGTCA CTCAGTCCTA CTGGGACACC AACCCCACGA GGGCTCAGGG GTACTCTGGA
    CTTTCCGTGA AGGTTGTAAA CTCCACCACG GGGCCTGGCG AGCACCTGCG GAATGCCCTG
    TGGCACACAG GAAACACCTC TGGCCAGGTG CGCACACTGT GGCATGACCC TCGTCACATT
    GGCTGGAAAG ATTTCACTGC CTACAGATGG CATCTGAGCC ACAGGCCAAA GACAGGTTTC
    ATCAGAGTGG TAATGTATGA AGGGAAGAAA ATCATGGCTG ACTCAGGACC CATCTATGAC
    AAAACCTATG CTGGTGGGAG GCTAGGCTTG TTCGTCTTCT CTCAAGAAAT GGTGTTCTTC
    TCCGACCTGA AATATGAATG CAGAGACTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.