MYH1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH1
Entrez Gene ID	281337
Full Name	myosin, heavy chain 1, skeletal muscle, adult
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024979592.1	XP_024835360.1	myosin-1 isoform X2
NM_174117.1	NP_776542.1	myosin-1
XM_010815981.2	XP_010814283.1	myosin-1 isoform X2
XM_010815980.2	XP_010814282.1	myosin-1 isoform X1
XM_010815980.3	XP_010814282.1	myosin-1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYH1	NP_005954.3
Xenopus tropicalis (tropical clawed frog)	myh8	XP_004916771.1
Xenopus tropicalis (tropical clawed frog)	LOC100495802	XP_002935255.1
Bos taurus (cattle)	MYH1	NP_776542.1
Xenopus tropicalis (tropical clawed frog)	myh4	NP_001001244.1
Xenopus tropicalis (tropical clawed frog)	myh2	NP_001006805.1
Xenopus tropicalis (tropical clawed frog)	LOC100495968	XP_002935256.2
Pan troglodytes (chimpanzee)	MYH1	XP_003953177.1
Canis lupus familiaris (dog)	MYH1	NP_001107189.1
Mus musculus (house mouse)	Myh1	NP_109604.1
Rattus norvegicus (Norway rat)	Myh1	NP_001128630.1
Xenopus tropicalis (tropical clawed frog)	LOC100495638	XP_002935254.1

Gene Ontology
Bibliography

Related articles in PubMed

Differences in mitochondrial gene expression profiles, enzyme activities and myosin heavy chain types in yak versus bovine skeletal muscles.
Lin YQ, Xu YO, Yue Y, Jin SY, Qu Y, Dong F, Li YP, Zheng YC
Genetics and molecular research : GMR11(3)2871-7(2012 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Myosin heavy chain isoforms expressed in bovine skeletal muscles.
Chikuni K, Muroya S, Nakajima I
Meat science67(1)87-94(2004 May)

Sequencing of the 2a, 2x, and slow isoforms of the bovine myosin heavy chain and the different expression among muscles.
Tanabe R, Muroya S, Chikuni K
Mammalian genome : official journal of the International Mammalian Genome Society9(12)1056-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The yak has significantly lower mRNA levels of CPT, UCP3, and MyHC 1 in biceps femoris than in Yellow cattle.
Title: Differences in mitochondrial gene expression profiles, enzyme activities and myosin heavy chain types in yak versus bovine skeletal muscles.

The following MYH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa225960	XM_024979592.1 Latest version!	Bos taurus myosin heavy chain 1 (MYH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa42053	NM_174117.1 Latest version!	Bos taurus myosin heavy chain 1 (MYH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa42053	XM_010815980.3 Latest version!	Bos taurus myosin heavy chain 1 (MYH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45121	XM_010815980.2 Latest version!	Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45122	XM_010815981.2 Latest version!	Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH1 ( XM_024979592.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835360.1 Bos taurus myosin heavy chain 1 (MYH1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa225960
Clone ID Related Accession (Same CDS sequence)	XM_024979592.1
Accession Version	XM_024979592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myosin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATAGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAGCAATGCA AATCTGTTGA GTGGGAAAGA 
GCCTGGTCTG AGAGGTGGAA AGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835360.1
CDS	1..5727
Translation	MSSDQEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVADPKESFVKATVQSREGGKVTAKTEAGATVTVKEDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDYAYVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEFVTKGQTVEQVYNAVGALAKAVYDKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDMSFKNKLYEQHLGKSNNFQKPKPAKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSVKTLALLFSGPASGEAEGGPKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKSEAKRKELEEKMVTLTQEKNDLQLQVQSEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALAMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSELKTKEDEQQRLINDLTTQRARLQTESGEFSRQLDEKDALVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSARHDCDLLREQYEEEQEGKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILSEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKVKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALKNYRSTQAILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDITQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQQCKSVEWERAWSERWKE

Target ORF information:

RefSeq Version	XM_024979592.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin heavy chain 1 (MYH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979592.1

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATAGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAGCAATGCA AATCTGTTGA GTGGGAAAGA 
GCCTGGTCTG AGAGGTGGAA AGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1 ( NM_174117.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776542.1 Bos taurus myosin heavy chain 1 (MYH1), mRNA.

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CloneID	OBa42053
Clone ID Related Accession (Same CDS sequence)	NM_174117.1 , XM_010815980.3
Accession Version	NM_174117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	myosin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB059399.2. ##Evidence-Data-START## Transcript exon combination :: AB059399.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATAGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAATCCAATG TCAACCTCTC CAAATTCCGC 
AAGCTCCAGC ACGAGCTGGA AGAGGCTGAG GAACGGGCTG ACATTGCCGA GTCCCAGGTC 
AACAAGCTGC GGGTGAAGAG CCGGGAGGTT CACACAAAAA TCATCAGTGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776542.1
CDS	64..5880
Translation	MSSDQEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVADPKESFVKATVQSREGGKVTAKTEAGATVTVKEDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDYAYVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEFVTKGQTVEQVYNAVGALAKAVYDKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDMSFKNKLYEQHLGKSNNFQKPKPAKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSVKTLALLFSGPASGEAEGGPKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKSEAKRKELEEKMVTLTQEKNDLQLQVQSEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALAMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSELKTKEDEQQRLINDLTTQRARLQTESGEFSRQLDEKDALVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSARHDCDLLREQYEEEQEGKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILSEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKVKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALKNYRSTQAILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDITQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNVNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	NM_174117.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin heavy chain 1 (MYH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174117.1

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATAGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAATCCAATG TCAACCTCTC CAAATTCCGC 
AAGCTCCAGC ACGAGCTGGA AGAGGCTGAG GAACGGGCTG ACATTGCCGA GTCCCAGGTC 
AACAAGCTGC GGGTGAAGAG CCGGGAGGTT CACACAAAAA TCATCAGTGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1 ( XM_010815980.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814282.1 Bos taurus myosin heavy chain 1 (MYH1), transcript variant X1, mRNA.

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CloneID	OBa42053
Clone ID Related Accession (Same CDS sequence)	NM_174117.1 , XM_010815980.3
Accession Version	XM_010815980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	myosin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATAGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAATCCAATG TCAACCTCTC CAAATTCCGC 
AAGCTCCAGC ACGAGCTGGA AGAGGCTGAG GAACGGGCTG ACATTGCCGA GTCCCAGGTC 
AACAAGCTGC GGGTGAAGAG CCGGGAGGTT CACACAAAAA TCATCAGTGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814282.1
CDS	134..5950
Translation	MSSDQEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVADPKESFVKATVQSREGGKVTAKTEAGATVTVKEDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDYAYVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEFVTKGQTVEQVYNAVGALAKAVYDKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDMSFKNKLYEQHLGKSNNFQKPKPAKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSVKTLALLFSGPASGEAEGGPKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKSEAKRKELEEKMVTLTQEKNDLQLQVQSEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALAMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSELKTKEDEQQRLINDLTTQRARLQTESGEFSRQLDEKDALVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSARHDCDLLREQYEEEQEGKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILSEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKVKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALKNYRSTQAILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDITQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNVNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	XM_010815980.3
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin heavy chain 1 (MYH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815980.3

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATAGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAATCCAATG TCAACCTCTC CAAATTCCGC 
AAGCTCCAGC ACGAGCTGGA AGAGGCTGAG GAACGGGCTG ACATTGCCGA GTCCCAGGTC 
AACAAGCTGC GGGTGAAGAG CCGGGAGGTT CACACAAAAA TCATCAGTGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1 ( XM_010815980.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814282.1 Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), transcript variant X1, mRNA.

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CloneID	OBa45121
Clone ID Related Accession (Same CDS sequence)	XM_010815980.2
Accession Version	XM_010815980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myosin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATCGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAATCCAATG TCAACCTCTC CAAATTCCGC 
AAGCTCCAGC ACGAGCTGGA AGAGGCTGAG GAACGGGCTG ACATTGCCGA GTCCCAGGTC 
AACAAGCTGC GGGTGAAGAG CCGGGAGGTT CACACAAAAA TCATCAGTGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814282.1
CDS	136..5952
Translation	MSSDQEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVADPKESFVKATVQSREGGKVTAKTEAGATVTVKEDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDYAYVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEFVTKGQTVEQVYNAVGALAKAVYDKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDMSFKNKLYEQHLGKSNNFQKPKPAKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSVKTLALLFSGPASGEAEGGPKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKSEAKRKELEEKMVTLTQEKNDLQLQVQSEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALAMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSELKTKEDEQQRLINDLTTQRARLQTESGEFSRQLDEKDALVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSARHDCDLLREQYEEEQEGKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILSEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKVKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALKNYRSTQAILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDITQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNVNLSKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKIISEE

Target ORF information:

RefSeq Version	XM_010815980.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815980.2

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATCGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAATCCAATG TCAACCTCTC CAAATTCCGC 
AAGCTCCAGC ACGAGCTGGA AGAGGCTGAG GAACGGGCTG ACATTGCCGA GTCCCAGGTC 
AACAAGCTGC GGGTGAAGAG CCGGGAGGTT CACACAAAAA TCATCAGTGA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH1 ( XM_010815981.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814283.1 Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), transcript variant X2, mRNA.

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CloneID	OBa45122
Clone ID Related Accession (Same CDS sequence)	XM_010815981.2
Accession Version	XM_010815981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	myosin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104494.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATCGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAGCAATGCA AATCTGTTGA GTGGGAAAGA 
GCCTGGTCTG AGAGGTGGAA AGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814283.1
CDS	137..5863
Translation	MSSDQEMAVFGEAAPYLRKSEKERIEAQNKPFDAKTSVFVADPKESFVKATVQSREGGKVTAKTEAGATVTVKEDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDYAYVSQGEITVPSIDDQEELMATDSAIEILGFTSDERVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQGLNSADLLKALCYPRVKVGNEFVTKGQTVEQVYNAVGALAKAVYDKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDMSFKNKLYEQHLGKSNNFQKPKPAKGKAEAHFSLIHYAGTVDYNITGWLDKNKDPLNETVVGLYQKSSVKTLALLFSGPASGEAEGGPKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDVDHTQYKFGHTKVFFKAGLLGLLEEMRDEKLAQLITRTQARCRGFLARVEYQKMVERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKSEAKRKELEEKMVTLTQEKNDLQLQVQSEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALAMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMETVSKAKGNLEKMCRALEDQLSELKTKEDEQQRLINDLTTQRARLQTESGEFSRQLDEKDALVSQLSRGKQAFTQQIEELKRQLEEEIKAKSALAHALQSARHDCDLLREQYEEEQEGKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNAKCASLEKTKQRLQNEVEDLMIDVERTNAACAALDKKQRNFDKILSEWKQKYEETHAELEASQKESRSLSTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKVKKQVEQEKSEIQAALEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALKNYRSTQAILKDTQIHLDDALRGQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDITQIQGEMEDIIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQQCKSVEWERAWSERWKE

Target ORF information:

RefSeq Version	XM_010815981.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myosin, heavy chain 1, skeletal muscle, adult (MYH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815981.2

ORF Insert Sequence:

ATGAGTTCAG ACCAGGAAAT GGCTGTTTTT GGGGAGGCCG CTCCTTACCT CCGAAAGTCT 
GAAAAGGAGC GCATTGAGGC CCAGAATAAG CCTTTCGATG CCAAGACCTC AGTCTTCGTG 
GCGGACCCTA AGGAGTCCTT CGTGAAAGCA ACTGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAGGCTGG GGCGACAGTA ACTGTGAAAG AAGACCAAGT CTTCCCCATG 
AACCCTCCCA AGTTTGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT GCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTACTCAGGC 
CTCTTCTGTG TCACCGTCAA CCCCTACAAG TGGCTGCCAG TGTACAATGC AGAGGTGGTG 
ACGGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGG GAGAATCAGT CTATCTTGAT CACCGGAGAA 
TCTGGGGCAG GAAAGACTGT GAACACGAAA CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACTGGGG AGAAGAAGAA GGAGGAACCA ACTTCAGGCA AAATGCAGGG GACTCTGGAG 
GATCAGATCA TCAGTGCCAA CCCCCTGCTG GAGGCTTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGGATCC ACTTTGGTAC CACAGGGAAA 
CTGGCTTCTG CTGATATTGA AACATATCTT CTGGAGAAGT CTAGAGTCAC TTTCCAGCTG 
AAGGCAGAAA GAAGCTACCA TATTTTTTAT CAGATCATGT CTAACAAGAA GCCAGAGCTA 
ATTGAAATGC TCCTAATCAC CACCAACCCA TATGACTATG CCTACGTGAG TCAAGGGGAG 
ATCACAGTCC CTAGCATTGA TGACCAAGAA GAGTTGATGG CCACAGATAG TGCCATTGAA 
ATCCTGGGCT TCACTTCTGA TGAAAGAGTG TCCATCTACA AGCTCACAGG GGCAGTAATG 
CATTATGGGA ATCTAAAATT CAAGCAAAAG CAGCGTGAGG AGCAAGCAGA ACCAGATGGC 
ACTGAAGTTG CTGACAAGGC TGCCTACCTC CAGGGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTTGGC AATGAGTTTG TCACCAAAGG CCAGACTGTA 
GAGCAGGTAT ATAACGCAGT GGGTGCTCTT GCCAAAGCCG TCTATGATAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG CTTTGAGATC TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGGAGTTCA TCGACTTCGG GATGGACCTG 
GCCGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCCAC AGACATGTCC TTCAAGAACA AGCTTTATGA ACAGCATCTT 
GGAAAGTCCA ACAACTTCCA GAAACCCAAA CCTGCCAAGG GCAAGGCTGA GGCCCACTTC 
TCCCTCATTC ACTATGCGGG CACTGTGGAC TACAACATTA CTGGCTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC GGTGGTCGGG CTGTACCAGA AGTCTTCAGT GAAGACTCTG 
GCTTTACTGT TCTCTGGCCC AGCATCTGGT GAAGCAGAGG GCGGTCCAAA GAAAGGTGGC 
AAGAAGAAGG GTTCTTCTTT CCAGACCGTG TCTGCTCTTT TCAGGGAGAA CCTGAATAAG 
CTGATGACCA ACCTGAGGAG CACTCACCCC CACTTTGTAC GCTGCATCAT CCCCAATGAA 
ACTAAAACTC CTGGGGCCAT GGAGCACGAG CTGGTCCTGC ACCAGCTGAG GTGTAATGGG 
GTGCTGGAGG GTATCCGCAT CTGCAGGAAG GGTTTCCCCA GCAGAATCCT GTACGCAGAC 
TTCAAACAGA GATACAAGGT ATTAAATGCA AGTGCGATCC CTGAAGGACA ATTCATTGAC 
AGCAAGAAGG CTTCTGAGAA GCTCCTTGCA TCCATTGACG TTGATCACAC CCAGTATAAA 
TTTGGTCACA CCAAGGTCTT TTTCAAAGCT GGTCTTCTGG GGCTCCTAGA GGAGATGCGA 
GATGAAAAGC TAGCCCAGCT GATTACCCGA ACCCAGGCCA GGTGCAGAGG GTTCTTGGCA 
AGAGTGGAGT ACCAGAAGAT GGTGGAGAGG AGAGAGTCCA TCTTCTGCAT CCAGTACAAT 
GTCCGTGCTT TCATGAACGT CAAGCACTGG CCTTGGATGA AGCTGTATTT CAAGATCAAG 
CCCCTCCTCA AGAGTGCAGA GACAGAGAAG GAAATGGCCA ACATGAAGGA AGAGTTTGAG 
AAGACCAAAG AAGAGCTGGC TAAGTCAGAA GCAAAAAGGA AAGAACTTGA AGAGAAAATG 
GTGACTCTGA CACAAGAGAA GAATGACCTG CAGCTCCAGG TTCAGTCTGA AGCTGATGCC 
TTGGCTGATG CAGAGGAAAG ATGTGACCAG CTGATTAAAA CCAAAATCCA GCTGGAGGCT 
AAGATCAAGG AGGTGACTGA GAGAGCCGAG GATGAGGAAG AGATCAATGC TGAGCTGACG 
GCCAAGAAGA GGAAACTGGA GGACGAATGT TCAGAACTAA AGAAAGATAT AGATGACCTT 
GAGCTGACAC TGGCCAAGGT TGAGAAGGAG AAACACGCCA CAGAGAATAA GGTGAAAAAC 
CTCACAGAAG AGATGGCAGG TCTGGATGAA ACCATTGCCA AGCTGACCAA GGAAAAGAAG 
GCCCTCCAGG AGGCCCACCA GCAGACCCTG GATGACCTAC AGGCAGAAGA GGACAAAGTC 
AACACTCTGA CCAAAGCTAA GACTAAGCTA GAGCAGCAAG TGGATGATCT TGAGGGGTCC 
TTGGAGCAAG AAAAGAAACT TCGCATGGAC CTAGAGAGAG CCAAGAGGAA ACTGGAGGGT 
GATCTAAAAC TGGCCCAAGA ATCCACAATG GATATAGAAA ATGATAAGCA GCAACTGGAT 
GAGAAACTCA AAAAGAAGGA GTTTGAAATG AGCAATCTGC AAAGCAAGAT TGAAGATGAA 
CAGGCCCTCG CGATGCAGCT GCAGAAGAAG ATCAAGGAGT TACAGGCCCG CATCGAGGAG 
CTGGAGGAGG AGATCGAGGC TGAGCGGGCC TCCCGCGCCA AAGCAGAGAA GCAGCGCTCA 
GACCTCTCCC GGGAACTGGA GGAGATCAGC GAGCGGCTGG AAGAAGCAGG CGGGGCCACT 
TCCGCCCAGA TTGAGATGAA CAAGAAGCGG GAGGCCGAGT TCCAGAAGAT GCGCAGGGAC 
CTGGAGGAGG CCACCCTGCA GCACGAGGCC ACGGCGGCCG CTCTTCGCAA GAAGCACGCG 
GACAGTGTGG CTGAGCTGGG GGAGCAGATT GACAACCTGC AGAGGGTCAA GCAGAAGCTG 
GAGAAGGAGA AGAGCGAGAT GAAGATGGAG ATCGACGACC TGGCCAGCAA CATGGAGACT 
GTCTCCAAAG CCAAGGGAAA CCTCGAAAAG ATGTGCCGCG CTCTAGAGGA TCAGCTGAGT 
GAACTTAAGA CAAAAGAGGA TGAGCAGCAG CGGCTGATCA ATGACCTAAC GACTCAGAGA 
GCGCGTCTGC AGACAGAATC AGGTGAATTC TCACGCCAGC TAGATGAAAA GGATGCACTC 
GTTTCTCAGC TCTCAAGGGG CAAACAAGCA TTCACACAAC AGATTGAAGA ACTGAAAAGG 
CAGCTGGAAG AGGAGATAAA GGCCAAGAGT GCCCTGGCCC ATGCCCTGCA GTCAGCCCGT 
CATGACTGTG ACCTGCTGCG GGAACAGTAT GAGGAGGAGC AGGAGGGCAA GGCCGAGCTG 
CAGAGGGCGA TGTCCAAGGC CAACAGCGAG GTGGCCCAGT GGAGGACCAA GTACGAGACG 
GACGCCATCC AGCGCACGGA GGAGCTGGAG GAGGCCAAGA AGAAGCTGGC CCAGCGTCTG 
CAGGATGCTG AGGAACACGT AGAAGCTGTG AACGCCAAAT GCGCCTCCCT TGAGAAGACC 
AAGCAGCGGC TCCAGAATGA GGTTGAGGAC CTCATGATTG ATGTGGAGAG GACAAACGCT 
GCCTGTGCAG CCCTGGACAA AAAGCAAAGG AACTTCGACA AGATCCTATC AGAATGGAAA 
CAGAAGTATG AAGAGACTCA TGCTGAACTT GAAGCCTCCC AGAAGGAGTC TCGCTCCCTT 
AGCACAGAGC TGTTTAAGAT CAAGAATGCC TATGAGGAGT CCTTAGACCA ACTGGAAACC 
TTGAAGCGGG AAAATAAGAA CTTGCAACAG GAGATCTCTG ACCTCACGGA GCAGATCGCA 
GAAGGAGGGA AACGTATCCA TGAACTGGAG AAAGTAAAGA AGCAAGTGGA ACAAGAGAAG 
TCTGAAATTC AGGCTGCTTT AGAGGAGGCA GAGGCATCTC TTGAACACGA AGAGGGCAAG 
ATCCTGCGTA TCCAGCTGGA GCTGAACCAG GTCAAGTCTG AAATTGACAG GAAAATTGCT 
GAAAAGGATG AGGAAATTGA CCAGCTGAAG AGGAACCACA TTAGAATCGT GGAGTCCATG 
CAGAGCACGT TGGATGCTGA AATCAGGAGC AGGAATGATG CCATCAGGCT CAAGAAGAAG 
ATGGAAGGAG ACCTCAATGA AATGGAGATC CAGTTGAACC ATGCCAACCG CATGGCTGCT 
GAGGCCCTGA AGAACTACAG GAGCACTCAA GCCATCCTCA AGGATACCCA GATCCACCTG 
GATGATGCTC TGCGGGGCCA GGAGGACTTG AAGGAGCAGC TGGCCATGGT GGAGCGCAGA 
GCCAACCTGC TGCAGGCTGA GATCGAGGAG CTGAGGGCCA CCCTGGAGCA GACAGAGAGG 
AGCAGGAAGA TCGCAGAGCA GGAGCTCCTG GATGCCAGTG AGCGTGTCCA GCTCCTGCAC 
ACCCAGAACA CCAGCCTGAT CAACACCAAG AAGAAGCTAG AGACAGACAT CACTCAAATC 
CAGGGAGAGA TGGAGGACAT TATCCAGGAA GCTCGCAACG CAGAAGAGAA AGCCAAGAAG 
GCCATCACTG ATGCAGCCAT GATGGCTGAG GAGCTAAAGA AGGAGCAGGA CACCAGTGCC 
CACCTGGAGC GGATGAAGAA GAACCTGGAG CAGACGGTGA AGGACCTGCA GCACCGCCTG 
GACGAAGCTG AGCAGCTGGC CCTGAAGGGC GGGAAGAAGC AGATCCAGAA GCTGGAGGCC 
AGGGTACGTG AACTGGAAGG AGAAGTTGAG AGTGAACAGA AACGCAATGT TGAAGCTGTC 
AAGGGTCTAC GCAAACACGA GAGAAGAGTG AAGGAGCTCA CTTACCAGAC TGAGGAGGAC 
CGCAAGAACA TTCTCAGGCT CCAGGATTTG GTGGATAAAC TGCAAGCAAA GGTGAAATCT 
TACAAGAGAC AAGCTGAGGA AGCGGAGGAA CAGCAATGCA AATCTGTTGA GTGGGAAAGA 
GCCTGGTCTG AGAGGTGGAA AGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differences in mitochondrial gene expression profiles, enzyme activities and myosin heavy chain types in yak versus bovine skeletal muscles.
Genetics and molecular research : GMR11(3)2871-7(2012 Aug)
Lin YQ,Xu YO,Yue Y,Jin SY,Qu Y,Dong F,Li YP,Zheng YC

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Myosin heavy chain isoforms expressed in bovine skeletal muscles.
Meat science67(1)87-94(2004 May)
Chikuni K,Muroya S,Nakajima I

Sequencing of the 2a, 2x, and slow isoforms of the bovine myosin heavy chain and the different expression among muscles.
Mammalian genome : official journal of the International Mammalian Genome Society9(12)1056-8(1998 Dec)
Tanabe R,Muroya S,Chikuni K

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MYH1 cDNA ORF clone, Bos taurus(cattle)

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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