ECE2 cDNA ORF clone, Bos taurus(cattle)

The following ECE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa15794 NM_001314064.1
Latest version!
Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2a, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OBa43340 NM_177958.3
Latest version!
Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2b, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa43801 NM_177957.3
Latest version!
Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2b, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa134036 NM_177956.3
Latest version!
Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2a, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa134037 NM_174046.4
Latest version!
Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2a, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa87755 XM_015472163.2
Latest version!
Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87756 XM_015472166.1
Latest version!
Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87757 XM_015472169.2
Latest version!
Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OBa87755 XM_015472163.1 Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa87757 XM_015472169.1 Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa15794
    Clone ID Related Accession (Same CDS sequence) NM_001314064.1
    Accession Version NM_001314064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 768bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform 2a-3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EV638202.1, BC142117.1 and NKLS02000001.1. On Oct 2, 2015 this sequence version replaced XM_005201577.2. Transcript Variant: This variant (2a-3) lacks several exons and includes an alternate 3' terminal exon, compared to variant 2a-1. It encodes isoform 2a-3 which is shorter and has a distinct C-terminus, compared to isoform 2a-1. ##Evidence-Data-START## Transcript exon combination :: BC142117.1, EV742031.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGCTTGTC TGGGGCCTTC CGCACAGGTG CCGGAGTTAC CAGAGAAGAA CTGCGGGTAC 
    CGCGAGGTCC AGTACTGGGA CCAGCGATAC CAGGGCGCTG CCGACTCTGC CCCCTACGAG
    TGGTTCGGAG ACTTCTCTTG CTTCCGTGAC CTCCTAGAGC CGGAGTTGCG GCCGTTGGAC
    CGTATCCTCG TGCTAGGCTG TGGAAACAGT GCCCTGAGCT ACGAGATATT CCTTGGGGGC
    TTCCCTGATG TGACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCCAT GAGGGCTCGG
    TATGCCCACG TGCCCACGCT GCGATGGGAG ACCATGGATG TGCGGGCACT GGGCTTCCCT
    AGTGGCTCTT TCGACGTGGT GCTTGAGAAG GGCACACTGG ATGCCCTGTT GACTGGTGAA
    CAGGATCCCT GGACTGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GCTAAATGAG
    GTGAGCCGAG TGCTGGTCCC TGCGGGTCGG TTCATCTCAT TGACCTCTGC TGCCCCCCAC
    TTTCGGACAA GACACTATGC TCAAGCTCAT TATGGCTGGT CCTTGAGGCA TGCAACCTAT
    GGCAATGGTT TCCAGTTTCA TTTCTACCTT ATGCAGAAGG GCAAAGAGCT CAGTGTGGCT
    CAGCTGGCCG TTGGGGCACA GATCCTCTCA CCCCCTAGAC CTCCCACCCC ATCCTGCTTC
    CTCCAGGACT CAGATCATGA GGACTTCCTC AGTGCCATTC AGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300993.1
    CDS33..800
    Translation

    Target ORF information:

    RefSeq Version NM_001314064.1
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001314064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGCTTGTC TGGGGCCTTC CGCACAGGTG CCGGAGTTAC CAGAGAAGAA CTGCGGGTAC 
    CGCGAGGTCC AGTACTGGGA CCAGCGATAC CAGGGCGCTG CCGACTCTGC CCCCTACGAG
    TGGTTCGGAG ACTTCTCTTG CTTCCGTGAC CTCCTAGAGC CGGAGTTGCG GCCGTTGGAC
    CGTATCCTCG TGCTAGGCTG TGGAAACAGT GCCCTGAGCT ACGAGATATT CCTTGGGGGC
    TTCCCTGATG TGACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCCAT GAGGGCTCGG
    TATGCCCACG TGCCCACGCT GCGATGGGAG ACCATGGATG TGCGGGCACT GGGCTTCCCT
    AGTGGCTCTT TCGACGTGGT GCTTGAGAAG GGCACACTGG ATGCCCTGTT GACTGGTGAA
    CAGGATCCCT GGACTGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GCTAAATGAG
    GTGAGCCGAG TGCTGGTCCC TGCGGGTCGG TTCATCTCAT TGACCTCTGC TGCCCCCCAC
    TTTCGGACAA GACACTATGC TCAAGCTCAT TATGGCTGGT CCTTGAGGCA TGCAACCTAT
    GGCAATGGTT TCCAGTTTCA TTTCTACCTT ATGCAGAAGG GCAAAGAGCT CAGTGTGGCT
    CAGCTGGCCG TTGGGGCACA GATCCTCTCA CCCCCTAGAC CTCCCACCCC ATCCTGCTTC
    CTCCAGGACT CAGATCATGA GGACTTCCTC AGTGCCATTC AGCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa43340
    Clone ID Related Accession (Same CDS sequence) NM_177958.3
    Accession Version NM_177958.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform 2b-2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Oct 1, 2015 this sequence version replaced NM_177958.2. Transcript Variant: This variant (2b-2) differs in the 5' UTR and coding region compared to variant 2a-2. The resulting isoform (2b-2) is shorter and has a distinct N-terminus compared to isoform 2a-2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF489576.1, SRR5571254.3297.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CAAACGCGCC 
    ACGCTGCGGG ATGAAGACGC TCCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC
    GTGGAGGCGG GATTCCGGAA GAGGACCAGT CGCCTCTTGG GGTTGCACAC CCAGCTGGAG
    CTGGTCTTGG CTGGTGTCTC TCTACTGCTG GCTGCCCTGC TTCTGGGTTG CTTGGTGGCC
    CTGGGGGTCC AGTACCACAG AGACCCATCC CATAGCACTT GCCTCACAGA GGCCTGCATT
    CGAGTGGCTG GAAAAATCCT GGAGTCCCTG GACCGTGGAG TGAGCCCCTG TGAGGACTTC
    TATCAGTTCT CCTGCGGAGG CTGGATTCGG AGAAACCCTC TACCTGATGG GCGTTCTCGC
    TGGAACACCT TCAACAGTCT CTGGGACCAG AATCAAGCCA TCCTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCCAG CAGTGAAGCT GAACGGAAGA CGCAGCGCTT CTACCTCTCC
    TGCTTACAGG TGGAGCGCAT CGAGGAGCTG GGTGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GCTGGAACGT TACGGGGCCC TGGGACCAGG ACAACTTCAT GGAGGTGCTG
    AAGGCAGTGG CAGGGACGTA TAGGGCCACC CCCTTCTTTA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGTT CCAACAGCAA TATTATCCAG GTGGACCAGT CTGGGCTCTT TCTACCCTCT
    CGAGATTACT ACCTAAACAG GACCGCCAAT GAGAAAGTGC TTACTGCCTA CCTGGACTAC
    ATGGAGGAGC TGGGGATGCT GCTGGGCGGA CAGCCAACCT CCACTCGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACGG TGCCCCAGGA CCAGCGGCGG
    GATGAGGAGA AGATCTACCA CAAGATGAGC ATCGCGGAGC TGCAGGCCCT GGCACCCTCC
    ATGGATTGGC TGGAGTTTCT GTCCTTCTTG CTGTCACCGC TGGAGCTGGG TGATTCTGAG
    CCTGTGGTGG TGTATGGGAC GGATTATTTG CAGCAGGTGT CGGAACTCAT CAACCGCACA
    GAGCCAAGTG TCCTGAACAA TTATCTGATC TGGAACCTGG TACAGAAGAC AACTTCAAGC
    CTGGACCACC GCTTTGAGTC TGCACAAGAA AAGCTGCTGG AGACCCTCTA TGGCACCAAG
    AAGTCCTGTA CACCGAGGTG GCAGACCTGC ATCTCCAACA CCGACGATGC TCTTGGCTTT
    GCTCTGGGCT CCCTCTTTGT GAAGGCCACA TTTGACCGGC AGAGCAAGGA AATTGCAGAG
    GGGATGATCA GCGAGATCCG AGCCGCCTTT GAGGAGGCTC TGGGACACTT GGTTTGGATG
    GACGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CCATCTATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA GCCCAAAGAG CTGGATGATG TTTATGATGG GTATGAAGTA
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ACTTCTCTGC TAAAGTGATG
    GCTGACCAGC TCCGCAAGCC TCCTAGCCGA GACCAGTGGA GCATGACCCC GCAGACAGTG
    AACGCCTACT ACCTTCCAAC CAAGAATGAA ATCGTCTTCC CTGCTGGCAT CCTGCAGGCC
    CCCTTCTACA CTTGCAACCA CCCCAAGGCC CTGAACTTCG GTGGCATCGG TGTGGTGATG
    GGCCACGAGT TGACACATGC CTTTGATGAC CAAGGGCGCG AGTATGACAA GGAAGGGAAT
    CTGCGGCCGT GGTGGCAGAA TGAGTCGTTG GCAGCCTTCC GGAACCACAC GGCCTGCATA
    GAGGAGCAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAAGC TCAACGGGCG CCAGACACTG
    GGGGAGAACA TTGCCGACAA TGGGGGGCTT AAGGCTGCCT ACAACGCTTA CAAAGCATGG
    CTAAGAAAGC ATGGGGAGGA GCAGCAGCTG CCAGCTGTGG GACTCACCAA CCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGTGGTGC TCGGTCCGCA CACCCGAGAG CTCTCACGAG
    GGGCTGGTGA CCGACCCCCA CAGCCCTGCC CGCTTCCGTG TGCTGGGCAC TCTCTCCAAC
    TCCCGTGACT TCCTGCGGCA CTTCGGCTGC CCTGTCGGCT CCCCCATGAA CTCAGGGCAG
    CTTTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808873.2
    CDS60..2357
    Translation

    Target ORF information:

    RefSeq Version NM_177958.3
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2b, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177958.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CAAACGCGCC 
    ACGCTGCGGG ATGAAGACGC TCCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC
    GTGGAGGCGG GATTCCGGAA GAGGACCAGT CGCCTCTTGG GGTTGCACAC CCAGCTGGAG
    CTGGTCTTGG CTGGTGTCTC TCTACTGCTG GCTGCCCTGC TTCTGGGTTG CTTGGTGGCC
    CTGGGGGTCC AGTACCACAG AGACCCATCC CATAGCACTT GCCTCACAGA GGCCTGCATT
    CGAGTGGCTG GAAAAATCCT GGAGTCCCTG GACCGTGGAG TGAGCCCCTG TGAGGACTTC
    TATCAGTTCT CCTGCGGAGG CTGGATTCGG AGAAACCCTC TACCTGATGG GCGTTCTCGC
    TGGAACACCT TCAACAGTCT CTGGGACCAG AATCAAGCCA TCCTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCCAG CAGTGAAGCT GAACGGAAGA CGCAGCGCTT CTACCTCTCC
    TGCTTACAGG TGGAGCGCAT CGAGGAGCTG GGTGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GCTGGAACGT TACGGGGCCC TGGGACCAGG ACAACTTCAT GGAGGTGCTG
    AAGGCAGTGG CAGGGACGTA TAGGGCCACC CCCTTCTTTA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGTT CCAACAGCAA TATTATCCAG GTGGACCAGT CTGGGCTCTT TCTACCCTCT
    CGAGATTACT ACCTAAACAG GACCGCCAAT GAGAAAGTGC TTACTGCCTA CCTGGACTAC
    ATGGAGGAGC TGGGGATGCT GCTGGGCGGA CAGCCAACCT CCACTCGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACGG TGCCCCAGGA CCAGCGGCGG
    GATGAGGAGA AGATCTACCA CAAGATGAGC ATCGCGGAGC TGCAGGCCCT GGCACCCTCC
    ATGGATTGGC TGGAGTTTCT GTCCTTCTTG CTGTCACCGC TGGAGCTGGG TGATTCTGAG
    CCTGTGGTGG TGTATGGGAC GGATTATTTG CAGCAGGTGT CGGAACTCAT CAACCGCACA
    GAGCCAAGTG TCCTGAACAA TTATCTGATC TGGAACCTGG TACAGAAGAC AACTTCAAGC
    CTGGACCACC GCTTTGAGTC TGCACAAGAA AAGCTGCTGG AGACCCTCTA TGGCACCAAG
    AAGTCCTGTA CACCGAGGTG GCAGACCTGC ATCTCCAACA CCGACGATGC TCTTGGCTTT
    GCTCTGGGCT CCCTCTTTGT GAAGGCCACA TTTGACCGGC AGAGCAAGGA AATTGCAGAG
    GGGATGATCA GCGAGATCCG AGCCGCCTTT GAGGAGGCTC TGGGACACTT GGTTTGGATG
    GACGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CCATCTATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA GCCCAAAGAG CTGGATGATG TTTATGATGG GTATGAAGTA
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ACTTCTCTGC TAAAGTGATG
    GCTGACCAGC TCCGCAAGCC TCCTAGCCGA GACCAGTGGA GCATGACCCC GCAGACAGTG
    AACGCCTACT ACCTTCCAAC CAAGAATGAA ATCGTCTTCC CTGCTGGCAT CCTGCAGGCC
    CCCTTCTACA CTTGCAACCA CCCCAAGGCC CTGAACTTCG GTGGCATCGG TGTGGTGATG
    GGCCACGAGT TGACACATGC CTTTGATGAC CAAGGGCGCG AGTATGACAA GGAAGGGAAT
    CTGCGGCCGT GGTGGCAGAA TGAGTCGTTG GCAGCCTTCC GGAACCACAC GGCCTGCATA
    GAGGAGCAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAAGC TCAACGGGCG CCAGACACTG
    GGGGAGAACA TTGCCGACAA TGGGGGGCTT AAGGCTGCCT ACAACGCTTA CAAAGCATGG
    CTAAGAAAGC ATGGGGAGGA GCAGCAGCTG CCAGCTGTGG GACTCACCAA CCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGTGGTGC TCGGTCCGCA CACCCGAGAG CTCTCACGAG
    GGGCTGGTGA CCGACCCCCA CAGCCCTGCC CGCTTCCGTG TGCTGGGCAC TCTCTCCAAC
    TCCCGTGACT TCCTGCGGCA CTTCGGCTGC CCTGTCGGCT CCCCCATGAA CTCAGGGCAG
    CTTTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa43801
    Clone ID Related Accession (Same CDS sequence) NM_177957.3
    Accession Version NM_177957.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform 2b-1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Oct 1, 2015 this sequence version replaced NM_177957.2. Transcript Variant: This variant (2b-1) differs in the 5' UTR and coding region compared to variant 2a-2. The resulting isoform (2b-1) is shorter and has a distinct N-terminus compared to isoform 2a-2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF489575.1, SRR5571263.37654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG CGGGATTCCG GAAGAGGACC 
    AGTCGCCTCT TGGGGTTGCA CACCCAGCTG GAGCTGGTCT TGGCTGGTGT CTCTCTACTG
    CTGGCTGCCC TGCTTCTGGG TTGCTTGGTG GCCCTGGGGG TCCAGTACCA CAGAGACCCA
    TCCCATAGCA CTTGCCTCAC AGAGGCCTGC ATTCGAGTGG CTGGAAAAAT CCTGGAGTCC
    CTGGACCGTG GAGTGAGCCC CTGTGAGGAC TTCTATCAGT TCTCCTGCGG AGGCTGGATT
    CGGAGAAACC CTCTACCTGA TGGGCGTTCT CGCTGGAACA CCTTCAACAG TCTCTGGGAC
    CAGAATCAAG CCATCCTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC CAGCAGTGAA
    GCTGAACGGA AGACGCAGCG CTTCTACCTC TCCTGCTTAC AGGTGGAGCG CATCGAGGAG
    CTGGGTGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGCTGGAA CGTTACGGGG
    CCCTGGGACC AGGACAACTT CATGGAGGTG CTGAAGGCAG TGGCAGGGAC GTATAGGGCC
    ACCCCCTTCT TTACTGTCTA CGTCAGTGCC GACTCTAAGA GTTCCAACAG CAATATTATC
    CAGGTGGACC AGTCTGGGCT CTTTCTACCC TCTCGAGATT ACTACCTAAA CAGGACCGCC
    AATGAGAAAG TGCTTACTGC CTACCTGGAC TACATGGAGG AGCTGGGGAT GCTGCTGGGC
    GGACAGCCAA CCTCCACTCG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CGGTGCCCCA GGACCAGCGG CGGGATGAGG AGAAGATCTA CCACAAGATG
    AGCATCGCGG AGCTGCAGGC CCTGGCACCC TCCATGGATT GGCTGGAGTT TCTGTCCTTC
    TTGCTGTCAC CGCTGGAGCT GGGTGATTCT GAGCCTGTGG TGGTGTATGG GACGGATTAT
    TTGCAGCAGG TGTCGGAACT CATCAACCGC ACAGAGCCAA GTGTCCTGAA CAATTATCTG
    ATCTGGAACC TGGTACAGAA GACAACTTCA AGCCTGGACC ACCGCTTTGA GTCTGCACAA
    GAAAAGCTGC TGGAGACCCT CTATGGCACC AAGAAGTCCT GTACACCGAG GTGGCAGACC
    TGCATCTCCA ACACCGACGA TGCTCTTGGC TTTGCTCTGG GCTCCCTCTT TGTGAAGGCC
    ACATTTGACC GGCAGAGCAA GGAAATTGCA GAGGGGATGA TCAGCGAGAT CCGAGCCGCC
    TTTGAGGAGG CTCTGGGACA CTTGGTTTGG ATGGACGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCCATCTA TGATATGATT GGTTTCCCGG ACTTCATCCT GGAGCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTATGAA GTATCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAACTTCTC TGCTAAAGTG ATGGCTGACC AGCTCCGCAA GCCTCCTAGC
    CGAGACCAGT GGAGCATGAC CCCGCAGACA GTGAACGCCT ACTACCTTCC AACCAAGAAT
    GAAATCGTCT TCCCTGCTGG CATCCTGCAG GCCCCCTTCT ACACTTGCAA CCACCCCAAG
    GCCCTGAACT TCGGTGGCAT CGGTGTGGTG ATGGGCCACG AGTTGACACA TGCCTTTGAT
    GACCAAGGGC GCGAGTATGA CAAGGAAGGG AATCTGCGGC CGTGGTGGCA GAATGAGTCG
    TTGGCAGCCT TCCGGAACCA CACGGCCTGC ATAGAGGAGC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA AGCTCAACGG GCGCCAGACA CTGGGGGAGA ACATTGCCGA CAATGGGGGG
    CTTAAGGCTG CCTACAACGC TTACAAAGCA TGGCTAAGAA AGCATGGGGA GGAGCAGCAG
    CTGCCAGCTG TGGGACTCAC CAACCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGTGG
    TGCTCGGTCC GCACACCCGA GAGCTCTCAC GAGGGGCTGG TGACCGACCC CCACAGCCCT
    GCCCGCTTCC GTGTGCTGGG CACTCTCTCC AACTCCCGTG ACTTCCTGCG GCACTTCGGC
    TGCCCTGTCG GCTCCCCCAT GAACTCAGGG CAGCTTTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808872.2
    CDS60..2270
    Translation

    Target ORF information:

    RefSeq Version NM_177957.3
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2b, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177957.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG CGGGATTCCG GAAGAGGACC 
    AGTCGCCTCT TGGGGTTGCA CACCCAGCTG GAGCTGGTCT TGGCTGGTGT CTCTCTACTG
    CTGGCTGCCC TGCTTCTGGG TTGCTTGGTG GCCCTGGGGG TCCAGTACCA CAGAGACCCA
    TCCCATAGCA CTTGCCTCAC AGAGGCCTGC ATTCGAGTGG CTGGAAAAAT CCTGGAGTCC
    CTGGACCGTG GAGTGAGCCC CTGTGAGGAC TTCTATCAGT TCTCCTGCGG AGGCTGGATT
    CGGAGAAACC CTCTACCTGA TGGGCGTTCT CGCTGGAACA CCTTCAACAG TCTCTGGGAC
    CAGAATCAAG CCATCCTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC CAGCAGTGAA
    GCTGAACGGA AGACGCAGCG CTTCTACCTC TCCTGCTTAC AGGTGGAGCG CATCGAGGAG
    CTGGGTGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGCTGGAA CGTTACGGGG
    CCCTGGGACC AGGACAACTT CATGGAGGTG CTGAAGGCAG TGGCAGGGAC GTATAGGGCC
    ACCCCCTTCT TTACTGTCTA CGTCAGTGCC GACTCTAAGA GTTCCAACAG CAATATTATC
    CAGGTGGACC AGTCTGGGCT CTTTCTACCC TCTCGAGATT ACTACCTAAA CAGGACCGCC
    AATGAGAAAG TGCTTACTGC CTACCTGGAC TACATGGAGG AGCTGGGGAT GCTGCTGGGC
    GGACAGCCAA CCTCCACTCG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CGGTGCCCCA GGACCAGCGG CGGGATGAGG AGAAGATCTA CCACAAGATG
    AGCATCGCGG AGCTGCAGGC CCTGGCACCC TCCATGGATT GGCTGGAGTT TCTGTCCTTC
    TTGCTGTCAC CGCTGGAGCT GGGTGATTCT GAGCCTGTGG TGGTGTATGG GACGGATTAT
    TTGCAGCAGG TGTCGGAACT CATCAACCGC ACAGAGCCAA GTGTCCTGAA CAATTATCTG
    ATCTGGAACC TGGTACAGAA GACAACTTCA AGCCTGGACC ACCGCTTTGA GTCTGCACAA
    GAAAAGCTGC TGGAGACCCT CTATGGCACC AAGAAGTCCT GTACACCGAG GTGGCAGACC
    TGCATCTCCA ACACCGACGA TGCTCTTGGC TTTGCTCTGG GCTCCCTCTT TGTGAAGGCC
    ACATTTGACC GGCAGAGCAA GGAAATTGCA GAGGGGATGA TCAGCGAGAT CCGAGCCGCC
    TTTGAGGAGG CTCTGGGACA CTTGGTTTGG ATGGACGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCCATCTA TGATATGATT GGTTTCCCGG ACTTCATCCT GGAGCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTATGAA GTATCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAACTTCTC TGCTAAAGTG ATGGCTGACC AGCTCCGCAA GCCTCCTAGC
    CGAGACCAGT GGAGCATGAC CCCGCAGACA GTGAACGCCT ACTACCTTCC AACCAAGAAT
    GAAATCGTCT TCCCTGCTGG CATCCTGCAG GCCCCCTTCT ACACTTGCAA CCACCCCAAG
    GCCCTGAACT TCGGTGGCAT CGGTGTGGTG ATGGGCCACG AGTTGACACA TGCCTTTGAT
    GACCAAGGGC GCGAGTATGA CAAGGAAGGG AATCTGCGGC CGTGGTGGCA GAATGAGTCG
    TTGGCAGCCT TCCGGAACCA CACGGCCTGC ATAGAGGAGC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA AGCTCAACGG GCGCCAGACA CTGGGGGAGA ACATTGCCGA CAATGGGGGG
    CTTAAGGCTG CCTACAACGC TTACAAAGCA TGGCTAAGAA AGCATGGGGA GGAGCAGCAG
    CTGCCAGCTG TGGGACTCAC CAACCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGTGG
    TGCTCGGTCC GCACACCCGA GAGCTCTCAC GAGGGGCTGG TGACCGACCC CCACAGCCCT
    GCCCGCTTCC GTGTGCTGGG CACTCTCTCC AACTCCCGTG ACTTCCTGCG GCACTTCGGC
    TGCCCTGTCG GCTCCCCCAT GAACTCAGGG CAGCTTTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa134036
    Clone ID Related Accession (Same CDS sequence) NM_177956.3
    Accession Version NM_177956.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform 2a-2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Oct 1, 2015 this sequence version replaced NM_177956.2. Transcript Variant: This variant (2a-2) represents the longest transcript and encodes the longest isoform (2a-2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF489574.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTTGTC TGGGGCCTTC CGCACAGGTG CCGGAGTTAC CAGAGAAGAA CTGCGGGTAC 
    CGCGAGGTCC AGTACTGGGA CCAGCGATAC CAGGGCGCTG CCGACTCTGC CCCCTACGAG
    TGGTTCGGAG ACTTCTCTTG CTTCCGTGAC CTCCTAGAGC CGGAGTTGCG GCCGTTGGAC
    CGTATCCTCG TGCTAGGCTG TGGAAACAGT GCCCTGAGCT ACGAGATATT CCTTGGGGGC
    TTCCCTGATG TGACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCCAT GAGGGCTCGG
    TATGCCCACG TGCCCACGCT GCGATGGGAG ACCATGGATG TGCGGGCACT GGGCTTCCCT
    AGTGGCTCTT TCGACGTGGT GCTTGAGAAG GGCACACTGG ATGCCCTGTT GACTGGTGAA
    CAGGATCCCT GGACTGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GCTAAATGAG
    ATGGTGGAGT ACAAACGCGC CACGCTGCGG GATGAAGACG CTCCCGAGAC CCCCGTAGAG
    GGCGGGGCCT CCCCGGACGC CGTGGAGGCG GGATTCCGGA AGAGGACCAG TCGCCTCTTG
    GGGTTGCACA CCCAGCTGGA GCTGGTCTTG GCTGGTGTCT CTCTACTGCT GGCTGCCCTG
    CTTCTGGGTT GCTTGGTGGC CCTGGGGGTC CAGTACCACA GAGACCCATC CCATAGCACT
    TGCCTCACAG AGGCCTGCAT TCGAGTGGCT GGAAAAATCC TGGAGTCCCT GGACCGTGGA
    GTGAGCCCCT GTGAGGACTT CTATCAGTTC TCCTGCGGAG GCTGGATTCG GAGAAACCCT
    CTACCTGATG GGCGTTCTCG CTGGAACACC TTCAACAGTC TCTGGGACCA GAATCAAGCC
    ATCCTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCCA GCAGTGAAGC TGAACGGAAG
    ACGCAGCGCT TCTACCTCTC CTGCTTACAG GTGGAGCGCA TCGAGGAGCT GGGTGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGCTGGAACG TTACGGGGCC CTGGGACCAG
    GACAACTTCA TGGAGGTGCT GAAGGCAGTG GCAGGGACGT ATAGGGCCAC CCCCTTCTTT
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGT TCCAACAGCA ATATTATCCA GGTGGACCAG
    TCTGGGCTCT TTCTACCCTC TCGAGATTAC TACCTAAACA GGACCGCCAA TGAGAAAGTG
    CTTACTGCCT ACCTGGACTA CATGGAGGAG CTGGGGATGC TGCTGGGCGG ACAGCCAACC
    TCCACTCGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACG
    GTGCCCCAGG ACCAGCGGCG GGATGAGGAG AAGATCTACC ACAAGATGAG CATCGCGGAG
    CTGCAGGCCC TGGCACCCTC CATGGATTGG CTGGAGTTTC TGTCCTTCTT GCTGTCACCG
    CTGGAGCTGG GTGATTCTGA GCCTGTGGTG GTGTATGGGA CGGATTATTT GCAGCAGGTG
    TCGGAACTCA TCAACCGCAC AGAGCCAAGT GTCCTGAACA ATTATCTGAT CTGGAACCTG
    GTACAGAAGA CAACTTCAAG CCTGGACCAC CGCTTTGAGT CTGCACAAGA AAAGCTGCTG
    GAGACCCTCT ATGGCACCAA GAAGTCCTGT ACACCGAGGT GGCAGACCTG CATCTCCAAC
    ACCGACGATG CTCTTGGCTT TGCTCTGGGC TCCCTCTTTG TGAAGGCCAC ATTTGACCGG
    CAGAGCAAGG AAATTGCAGA GGGGATGATC AGCGAGATCC GAGCCGCCTT TGAGGAGGCT
    CTGGGACACT TGGTTTGGAT GGACGAGAAG ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT
    GCCATCTATG ATATGATTGG TTTCCCGGAC TTCATCCTGG AGCCCAAAGA GCTGGATGAT
    GTTTATGATG GGTATGAAGT ATCTGAAGAT TCCTTCTTCC AGAACATGTT GAATTTGTAC
    AACTTCTCTG CTAAAGTGAT GGCTGACCAG CTCCGCAAGC CTCCTAGCCG AGACCAGTGG
    AGCATGACCC CGCAGACAGT GAACGCCTAC TACCTTCCAA CCAAGAATGA AATCGTCTTC
    CCTGCTGGCA TCCTGCAGGC CCCCTTCTAC ACTTGCAACC ACCCCAAGGC CCTGAACTTC
    GGTGGCATCG GTGTGGTGAT GGGCCACGAG TTGACACATG CCTTTGATGA CCAAGGGCGC
    GAGTATGACA AGGAAGGGAA TCTGCGGCCG TGGTGGCAGA ATGAGTCGTT GGCAGCCTTC
    CGGAACCACA CGGCCTGCAT AGAGGAGCAG TACAGCCAGT ACCAGGTCAA CGGGGAGAAG
    CTCAACGGGC GCCAGACACT GGGGGAGAAC ATTGCCGACA ATGGGGGGCT TAAGGCTGCC
    TACAACGCTT ACAAAGCATG GCTAAGAAAG CATGGGGAGG AGCAGCAGCT GCCAGCTGTG
    GGACTCACCA ACCACCAGCT CTTCTTTGTG GGATTTGCCC AGGTGTGGTG CTCGGTCCGC
    ACACCCGAGA GCTCTCACGA GGGGCTGGTG ACCGACCCCC ACAGCCCTGC CCGCTTCCGT
    GTGCTGGGCA CTCTCTCCAA CTCCCGTGAC TTCCTGCGGC ACTTCGGCTG CCCTGTCGGC
    TCCCCCATGA ACTCAGGGCA GCTTTGTGAG GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_808871.3
    CDS33..2771
    Translation

    Target ORF information:

    RefSeq Version NM_177956.3
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177956.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTTGTC TGGGGCCTTC CGCACAGGTG CCGGAGTTAC CAGAGAAGAA CTGCGGGTAC 
    CGCGAGGTCC AGTACTGGGA CCAGCGATAC CAGGGCGCTG CCGACTCTGC CCCCTACGAG
    TGGTTCGGAG ACTTCTCTTG CTTCCGTGAC CTCCTAGAGC CGGAGTTGCG GCCGTTGGAC
    CGTATCCTCG TGCTAGGCTG TGGAAACAGT GCCCTGAGCT ACGAGATATT CCTTGGGGGC
    TTCCCTGATG TGACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCCAT GAGGGCTCGG
    TATGCCCACG TGCCCACGCT GCGATGGGAG ACCATGGATG TGCGGGCACT GGGCTTCCCT
    AGTGGCTCTT TCGACGTGGT GCTTGAGAAG GGCACACTGG ATGCCCTGTT GACTGGTGAA
    CAGGATCCCT GGACTGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GCTAAATGAG
    ATGGTGGAGT ACAAACGCGC CACGCTGCGG GATGAAGACG CTCCCGAGAC CCCCGTAGAG
    GGCGGGGCCT CCCCGGACGC CGTGGAGGCG GGATTCCGGA AGAGGACCAG TCGCCTCTTG
    GGGTTGCACA CCCAGCTGGA GCTGGTCTTG GCTGGTGTCT CTCTACTGCT GGCTGCCCTG
    CTTCTGGGTT GCTTGGTGGC CCTGGGGGTC CAGTACCACA GAGACCCATC CCATAGCACT
    TGCCTCACAG AGGCCTGCAT TCGAGTGGCT GGAAAAATCC TGGAGTCCCT GGACCGTGGA
    GTGAGCCCCT GTGAGGACTT CTATCAGTTC TCCTGCGGAG GCTGGATTCG GAGAAACCCT
    CTACCTGATG GGCGTTCTCG CTGGAACACC TTCAACAGTC TCTGGGACCA GAATCAAGCC
    ATCCTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCCA GCAGTGAAGC TGAACGGAAG
    ACGCAGCGCT TCTACCTCTC CTGCTTACAG GTGGAGCGCA TCGAGGAGCT GGGTGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGCTGGAACG TTACGGGGCC CTGGGACCAG
    GACAACTTCA TGGAGGTGCT GAAGGCAGTG GCAGGGACGT ATAGGGCCAC CCCCTTCTTT
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGT TCCAACAGCA ATATTATCCA GGTGGACCAG
    TCTGGGCTCT TTCTACCCTC TCGAGATTAC TACCTAAACA GGACCGCCAA TGAGAAAGTG
    CTTACTGCCT ACCTGGACTA CATGGAGGAG CTGGGGATGC TGCTGGGCGG ACAGCCAACC
    TCCACTCGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACG
    GTGCCCCAGG ACCAGCGGCG GGATGAGGAG AAGATCTACC ACAAGATGAG CATCGCGGAG
    CTGCAGGCCC TGGCACCCTC CATGGATTGG CTGGAGTTTC TGTCCTTCTT GCTGTCACCG
    CTGGAGCTGG GTGATTCTGA GCCTGTGGTG GTGTATGGGA CGGATTATTT GCAGCAGGTG
    TCGGAACTCA TCAACCGCAC AGAGCCAAGT GTCCTGAACA ATTATCTGAT CTGGAACCTG
    GTACAGAAGA CAACTTCAAG CCTGGACCAC CGCTTTGAGT CTGCACAAGA AAAGCTGCTG
    GAGACCCTCT ATGGCACCAA GAAGTCCTGT ACACCGAGGT GGCAGACCTG CATCTCCAAC
    ACCGACGATG CTCTTGGCTT TGCTCTGGGC TCCCTCTTTG TGAAGGCCAC ATTTGACCGG
    CAGAGCAAGG AAATTGCAGA GGGGATGATC AGCGAGATCC GAGCCGCCTT TGAGGAGGCT
    CTGGGACACT TGGTTTGGAT GGACGAGAAG ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT
    GCCATCTATG ATATGATTGG TTTCCCGGAC TTCATCCTGG AGCCCAAAGA GCTGGATGAT
    GTTTATGATG GGTATGAAGT ATCTGAAGAT TCCTTCTTCC AGAACATGTT GAATTTGTAC
    AACTTCTCTG CTAAAGTGAT GGCTGACCAG CTCCGCAAGC CTCCTAGCCG AGACCAGTGG
    AGCATGACCC CGCAGACAGT GAACGCCTAC TACCTTCCAA CCAAGAATGA AATCGTCTTC
    CCTGCTGGCA TCCTGCAGGC CCCCTTCTAC ACTTGCAACC ACCCCAAGGC CCTGAACTTC
    GGTGGCATCG GTGTGGTGAT GGGCCACGAG TTGACACATG CCTTTGATGA CCAAGGGCGC
    GAGTATGACA AGGAAGGGAA TCTGCGGCCG TGGTGGCAGA ATGAGTCGTT GGCAGCCTTC
    CGGAACCACA CGGCCTGCAT AGAGGAGCAG TACAGCCAGT ACCAGGTCAA CGGGGAGAAG
    CTCAACGGGC GCCAGACACT GGGGGAGAAC ATTGCCGACA ATGGGGGGCT TAAGGCTGCC
    TACAACGCTT ACAAAGCATG GCTAAGAAAG CATGGGGAGG AGCAGCAGCT GCCAGCTGTG
    GGACTCACCA ACCACCAGCT CTTCTTTGTG GGATTTGCCC AGGTGTGGTG CTCGGTCCGC
    ACACCCGAGA GCTCTCACGA GGGGCTGGTG ACCGACCCCC ACAGCCCTGC CCGCTTCCGT
    GTGCTGGGCA CTCTCTCCAA CTCCCGTGAC TTCCTGCGGC ACTTCGGCTG CCCTGTCGGC
    TCCCCCATGA ACTCAGGGCA GCTTTGTGAG GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa134037
    Clone ID Related Accession (Same CDS sequence) NM_174046.4
    Accession Version NM_174046.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform 2a-1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000001.1. On Oct 1, 2015 this sequence version replaced NM_174046.3. Transcript Variant: This variant (2a-1) lacks an alternate in-frame exon compared to variant 2a-2. The resulting isoform (2a-1) has the same N- and C-termini but is shorter compared to isoform 2a-2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF489573.1, U27341.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTTGTC TGGGGCCTTC CGCACAGGTG CCGGAGTTAC CAGAGAAGAA CTGCGGGTAC 
    CGCGAGGTCC AGTACTGGGA CCAGCGATAC CAGGGCGCTG CCGACTCTGC CCCCTACGAG
    TGGTTCGGAG ACTTCTCTTG CTTCCGTGAC CTCCTAGAGC CGGAGTTGCG GCCGTTGGAC
    CGTATCCTCG TGCTAGGCTG TGGAAACAGT GCCCTGAGCT ACGAGATATT CCTTGGGGGC
    TTCCCTGATG TGACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCCAT GAGGGCTCGG
    TATGCCCACG TGCCCACGCT GCGATGGGAG ACCATGGATG TGCGGGCACT GGGCTTCCCT
    AGTGGCTCTT TCGACGTGGT GCTTGAGAAG GGCACACTGG ATGCCCTGTT GACTGGTGAA
    CAGGATCCCT GGACTGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GCTAAATGAG
    GCGGGATTCC GGAAGAGGAC CAGTCGCCTC TTGGGGTTGC ACACCCAGCT GGAGCTGGTC
    TTGGCTGGTG TCTCTCTACT GCTGGCTGCC CTGCTTCTGG GTTGCTTGGT GGCCCTGGGG
    GTCCAGTACC ACAGAGACCC ATCCCATAGC ACTTGCCTCA CAGAGGCCTG CATTCGAGTG
    GCTGGAAAAA TCCTGGAGTC CCTGGACCGT GGAGTGAGCC CCTGTGAGGA CTTCTATCAG
    TTCTCCTGCG GAGGCTGGAT TCGGAGAAAC CCTCTACCTG ATGGGCGTTC TCGCTGGAAC
    ACCTTCAACA GTCTCTGGGA CCAGAATCAA GCCATCCTGA AGCACCTGCT TGAAAACACC
    ACCTTCAACT CCAGCAGTGA AGCTGAACGG AAGACGCAGC GCTTCTACCT CTCCTGCTTA
    CAGGTGGAGC GCATCGAGGA GCTGGGTGCC CAGCCACTGC GAGACCTCAT TGACAAGATT
    GGTGGCTGGA ACGTTACGGG GCCCTGGGAC CAGGACAACT TCATGGAGGT GCTGAAGGCA
    GTGGCAGGGA CGTATAGGGC CACCCCCTTC TTTACTGTCT ACGTCAGTGC CGACTCTAAG
    AGTTCCAACA GCAATATTAT CCAGGTGGAC CAGTCTGGGC TCTTTCTACC CTCTCGAGAT
    TACTACCTAA ACAGGACCGC CAATGAGAAA GTGCTTACTG CCTACCTGGA CTACATGGAG
    GAGCTGGGGA TGCTGCTGGG CGGACAGCCA ACCTCCACTC GGGAGCAGAT GCGGCAGGTG
    CTGGAGCTGG AGATACAACT GGCCAACATC ACGGTGCCCC AGGACCAGCG GCGGGATGAG
    GAGAAGATCT ACCACAAGAT GAGCATCGCG GAGCTGCAGG CCCTGGCACC CTCCATGGAT
    TGGCTGGAGT TTCTGTCCTT CTTGCTGTCA CCGCTGGAGC TGGGTGATTC TGAGCCTGTG
    GTGGTGTATG GGACGGATTA TTTGCAGCAG GTGTCGGAAC TCATCAACCG CACAGAGCCA
    AGTGTCCTGA ACAATTATCT GATCTGGAAC CTGGTACAGA AGACAACTTC AAGCCTGGAC
    CACCGCTTTG AGTCTGCACA AGAAAAGCTG CTGGAGACCC TCTATGGCAC CAAGAAGTCC
    TGTACACCGA GGTGGCAGAC CTGCATCTCC AACACCGACG ATGCTCTTGG CTTTGCTCTG
    GGCTCCCTCT TTGTGAAGGC CACATTTGAC CGGCAGAGCA AGGAAATTGC AGAGGGGATG
    ATCAGCGAGA TCCGAGCCGC CTTTGAGGAG GCTCTGGGAC ACTTGGTTTG GATGGACGAG
    AAGACCCGCC AGGCAGCCAA GGAGAAAGCA GATGCCATCT ATGATATGAT TGGTTTCCCG
    GACTTCATCC TGGAGCCCAA AGAGCTGGAT GATGTTTATG ATGGGTATGA AGTATCTGAA
    GATTCCTTCT TCCAGAACAT GTTGAATTTG TACAACTTCT CTGCTAAAGT GATGGCTGAC
    CAGCTCCGCA AGCCTCCTAG CCGAGACCAG TGGAGCATGA CCCCGCAGAC AGTGAACGCC
    TACTACCTTC CAACCAAGAA TGAAATCGTC TTCCCTGCTG GCATCCTGCA GGCCCCCTTC
    TACACTTGCA ACCACCCCAA GGCCCTGAAC TTCGGTGGCA TCGGTGTGGT GATGGGCCAC
    GAGTTGACAC ATGCCTTTGA TGACCAAGGG CGCGAGTATG ACAAGGAAGG GAATCTGCGG
    CCGTGGTGGC AGAATGAGTC GTTGGCAGCC TTCCGGAACC ACACGGCCTG CATAGAGGAG
    CAGTACAGCC AGTACCAGGT CAACGGGGAG AAGCTCAACG GGCGCCAGAC ACTGGGGGAG
    AACATTGCCG ACAATGGGGG GCTTAAGGCT GCCTACAACG CTTACAAAGC ATGGCTAAGA
    AAGCATGGGG AGGAGCAGCA GCTGCCAGCT GTGGGACTCA CCAACCACCA GCTCTTCTTT
    GTGGGATTTG CCCAGGTGTG GTGCTCGGTC CGCACACCCG AGAGCTCTCA CGAGGGGCTG
    GTGACCGACC CCCACAGCCC TGCCCGCTTC CGTGTGCTGG GCACTCTCTC CAACTCCCGT
    GACTTCCTGC GGCACTTCGG CTGCCCTGTC GGCTCCCCCA TGAACTCAGG GCAGCTTTGT
    GAGGTGTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776471.3
    CDS33..2684
    Translation

    Target ORF information:

    RefSeq Version NM_174046.4
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant 2a, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174046.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCTTGTC TGGGGCCTTC CGCACAGGTG CCGGAGTTAC CAGAGAAGAA CTGCGGGTAC 
    CGCGAGGTCC AGTACTGGGA CCAGCGATAC CAGGGCGCTG CCGACTCTGC CCCCTACGAG
    TGGTTCGGAG ACTTCTCTTG CTTCCGTGAC CTCCTAGAGC CGGAGTTGCG GCCGTTGGAC
    CGTATCCTCG TGCTAGGCTG TGGAAACAGT GCCCTGAGCT ACGAGATATT CCTTGGGGGC
    TTCCCTGATG TGACCAGTGT GGACTACTCA TCAGTAGTGG TGGCTGCCAT GAGGGCTCGG
    TATGCCCACG TGCCCACGCT GCGATGGGAG ACCATGGATG TGCGGGCACT GGGCTTCCCT
    AGTGGCTCTT TCGACGTGGT GCTTGAGAAG GGCACACTGG ATGCCCTGTT GACTGGTGAA
    CAGGATCCCT GGACTGTGTC CTCTGAAGGT GTCCACACTG TGGACCAGGT GCTAAATGAG
    GCGGGATTCC GGAAGAGGAC CAGTCGCCTC TTGGGGTTGC ACACCCAGCT GGAGCTGGTC
    TTGGCTGGTG TCTCTCTACT GCTGGCTGCC CTGCTTCTGG GTTGCTTGGT GGCCCTGGGG
    GTCCAGTACC ACAGAGACCC ATCCCATAGC ACTTGCCTCA CAGAGGCCTG CATTCGAGTG
    GCTGGAAAAA TCCTGGAGTC CCTGGACCGT GGAGTGAGCC CCTGTGAGGA CTTCTATCAG
    TTCTCCTGCG GAGGCTGGAT TCGGAGAAAC CCTCTACCTG ATGGGCGTTC TCGCTGGAAC
    ACCTTCAACA GTCTCTGGGA CCAGAATCAA GCCATCCTGA AGCACCTGCT TGAAAACACC
    ACCTTCAACT CCAGCAGTGA AGCTGAACGG AAGACGCAGC GCTTCTACCT CTCCTGCTTA
    CAGGTGGAGC GCATCGAGGA GCTGGGTGCC CAGCCACTGC GAGACCTCAT TGACAAGATT
    GGTGGCTGGA ACGTTACGGG GCCCTGGGAC CAGGACAACT TCATGGAGGT GCTGAAGGCA
    GTGGCAGGGA CGTATAGGGC CACCCCCTTC TTTACTGTCT ACGTCAGTGC CGACTCTAAG
    AGTTCCAACA GCAATATTAT CCAGGTGGAC CAGTCTGGGC TCTTTCTACC CTCTCGAGAT
    TACTACCTAA ACAGGACCGC CAATGAGAAA GTGCTTACTG CCTACCTGGA CTACATGGAG
    GAGCTGGGGA TGCTGCTGGG CGGACAGCCA ACCTCCACTC GGGAGCAGAT GCGGCAGGTG
    CTGGAGCTGG AGATACAACT GGCCAACATC ACGGTGCCCC AGGACCAGCG GCGGGATGAG
    GAGAAGATCT ACCACAAGAT GAGCATCGCG GAGCTGCAGG CCCTGGCACC CTCCATGGAT
    TGGCTGGAGT TTCTGTCCTT CTTGCTGTCA CCGCTGGAGC TGGGTGATTC TGAGCCTGTG
    GTGGTGTATG GGACGGATTA TTTGCAGCAG GTGTCGGAAC TCATCAACCG CACAGAGCCA
    AGTGTCCTGA ACAATTATCT GATCTGGAAC CTGGTACAGA AGACAACTTC AAGCCTGGAC
    CACCGCTTTG AGTCTGCACA AGAAAAGCTG CTGGAGACCC TCTATGGCAC CAAGAAGTCC
    TGTACACCGA GGTGGCAGAC CTGCATCTCC AACACCGACG ATGCTCTTGG CTTTGCTCTG
    GGCTCCCTCT TTGTGAAGGC CACATTTGAC CGGCAGAGCA AGGAAATTGC AGAGGGGATG
    ATCAGCGAGA TCCGAGCCGC CTTTGAGGAG GCTCTGGGAC ACTTGGTTTG GATGGACGAG
    AAGACCCGCC AGGCAGCCAA GGAGAAAGCA GATGCCATCT ATGATATGAT TGGTTTCCCG
    GACTTCATCC TGGAGCCCAA AGAGCTGGAT GATGTTTATG ATGGGTATGA AGTATCTGAA
    GATTCCTTCT TCCAGAACAT GTTGAATTTG TACAACTTCT CTGCTAAAGT GATGGCTGAC
    CAGCTCCGCA AGCCTCCTAG CCGAGACCAG TGGAGCATGA CCCCGCAGAC AGTGAACGCC
    TACTACCTTC CAACCAAGAA TGAAATCGTC TTCCCTGCTG GCATCCTGCA GGCCCCCTTC
    TACACTTGCA ACCACCCCAA GGCCCTGAAC TTCGGTGGCA TCGGTGTGGT GATGGGCCAC
    GAGTTGACAC ATGCCTTTGA TGACCAAGGG CGCGAGTATG ACAAGGAAGG GAATCTGCGG
    CCGTGGTGGC AGAATGAGTC GTTGGCAGCC TTCCGGAACC ACACGGCCTG CATAGAGGAG
    CAGTACAGCC AGTACCAGGT CAACGGGGAG AAGCTCAACG GGCGCCAGAC ACTGGGGGAG
    AACATTGCCG ACAATGGGGG GCTTAAGGCT GCCTACAACG CTTACAAAGC ATGGCTAAGA
    AAGCATGGGG AGGAGCAGCA GCTGCCAGCT GTGGGACTCA CCAACCACCA GCTCTTCTTT
    GTGGGATTTG CCCAGGTGTG GTGCTCGGTC CGCACACCCG AGAGCTCTCA CGAGGGGCTG
    GTGACCGACC CCCACAGCCC TGCCCGCTTC CGTGTGCTGG GCACTCTCTC CAACTCCCGT
    GACTTCCTGC GGCACTTCGG CTGCCCTGTC GGCTCCCCCA TGAACTCAGG GCAGCTTTGT
    GAGGTGTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87755
    Clone ID Related Accession (Same CDS sequence) XM_015472163.1 , XM_015472163.2
    Accession Version XM_015472163.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CAAACGCGCC 
    ACGCTGCGGG ATGAAGACGC TCCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC
    GTGGAGGCGG GATTCCGGAA GAGGACCAGT CGCCTCTTGG GGTTGCACAC CCAGCTGGAG
    CTGGTCTTGG CTGGTGTCTC TCTACTGCTG GCTGCCCTGC TTCTGGGTTG CTTGGTGGCC
    CTGGGGGTCC AGTACCACAG AGACCCATCC CATAGCACTT GCCTCACAGA GGCCTGCATT
    CGAGTGGCTG GAAAAATCCT GGAGTCCCTG GACCGTGGAG TGAGCCCCTG TGAGGACTTC
    TATCAGTTCT CCTGCGGAGG CTGGATTCGG AGAAACCCTC TACCTGATGG GCGTTCTCGC
    TGGAACACCT TCAACAGTCT CTGGGACCAG AATCAAGCCA TCCTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCCAG CAGTGAAGCT GAACGGAAGA CGCAGCGCTT CTACCTCTCC
    TGCTTACAGG TGGAGCGCAT CGAGGAGCTG GGTGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GCTGGAACGT TACGGGGCCC TGGGACCAGG ACAACTTCAT GGAGGTGCTG
    AAGGCAGTGG CAGGGACGTA TAGGGCCACC CCCTTCTTTA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGTT CCAACAGCAA TATTATCCAG GTGGACCAGT CTGGGCTCTT TCTACCCTCT
    CGAGATTACT ACCTAAACAG GACCGCCAAT GAGAAAGTGC TTACTGCCTA CCTGGACTAC
    ATGGAGGAGC TGGGGATGCT GCTGGGCGGA CAGCCAACCT CCACTCGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACGG TGCCCCAGGA CCAGCGGCGG
    GATGAGGAGA AGATCTACCA CAAGATGAGC ATCGCGGAGC TGCAGGCCCT GGCACCCTCC
    ATGGATTGGC TGGAGTTTCT GTCCTTCTTG CTGTCACCGC TGGAGCTGGG TGATTCTGAG
    CCTGTGGTGG TGTATGGGAC GGATTATTTG CAGCAGGTGT CGGAACTCAT CAACCGCACA
    GAGCCAAGTG TCCTGAACAA TTATCTGATC TGGAACCTGG TACAGAAGAC AACTTCAAGC
    CTGGACCACC GCTTTGAGTC TGCACAAGAA AAGCTGCTGG AGACCCTCTA TGGCACCAAG
    AAGTCCTGTA CACCGAGGTG GCAGACCTGC ATCTCCAACA CCGACGATGC TCTTGGCTTT
    GCTCTGGGCT CCCTCTTTGT GAAGGCCACA TTTGACCGGC AGAGCAAGGA AATTGCAGAG
    GGGATGATCA GCGAGATCCG AGCCGCCTTT GAGGAGGCTC TGGGACACTT GGTTTGGATG
    GACGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CCATCTATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA GCCCAAAGAG CTGGATGATG TTTATGATGG GTATGAAGTA
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ACTTCTCTGC TAAAGTGATG
    GCTGACCAGC TCCGCAAGCC TCCTAGCCGA GACCAGTGGA GCATGACCCC GCAGACAGTG
    AACGCCTACT ACCTTCCAAC CAAGAATGAA ATCGTCTTCC CTGCTGGCAT CCTGCAGGCC
    CCCTTCTACA CTTGCAACCA CCCCAAGGCC CTGAACTTCG GTGGCATCGG TGTGGTGATG
    GGCCACGAGT TGACACATGC CTTTGATGAC CAAGGGCGCG AGTATGACAA GGAAGGGAAT
    CTGCGGCCGT GGTGGCAGAA TGAGTCGTTG GCAGCCTTCC GGAACCACAC GGCCTGCATA
    GAGGAGCAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAAGC TCAACGGGCG CCAGACACTG
    GGGGAGAACA TTGCCGACAA TGGGGGGCTT AAGGCTGCCT ACAACGCTTA CAAAGCATGG
    CTAAGAAAGC ATGGGGAGGA GCAGCAGCTG CCAGCTGTGG GACTCACCAA CCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGGCTGTG GAGGAGGAAG CTCGGGGACA GAGTGGCTCG
    TCCAGGGGTA CCCAGGAAGA CAGAAAGTGG CAGACTCAGC AAGGTCTCCA GGTGTGGTGC
    TCGGTCCGCA CACCCGAGAG CTCTCACGAG GGGCTGGTGA CCGACCCCCA CAGCCCTGCC
    CGCTTCCGTG TGCTGGGCAC TCTCTCCAAC TCCCGTGACT TCCTGCGGCA CTTCGGCTGC
    CCTGTCGGCT CCCCCATGAA CTCAGGGCAG CTTTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327649.1
    CDS75..2462
    Translation

    Target ORF information:

    RefSeq Version XM_015472163.1
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CAAACGCGCC 
    ACGCTGCGGG ATGAAGACGC TCCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC
    GTGGAGGCGG GATTCCGGAA GAGGACCAGT CGCCTCTTGG GGTTGCACAC CCAGCTGGAG
    CTGGTCTTGG CTGGTGTCTC TCTACTGCTG GCTGCCCTGC TTCTGGGTTG CTTGGTGGCC
    CTGGGGGTCC AGTACCACAG AGACCCATCC CATAGCACTT GCCTCACAGA GGCCTGCATT
    CGAGTGGCTG GAAAAATCCT GGAGTCCCTG GACCGTGGAG TGAGCCCCTG TGAGGACTTC
    TATCAGTTCT CCTGCGGAGG CTGGATTCGG AGAAACCCTC TACCTGATGG GCGTTCTCGC
    TGGAACACCT TCAACAGTCT CTGGGACCAG AATCAAGCCA TCCTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCCAG CAGTGAAGCT GAACGGAAGA CGCAGCGCTT CTACCTCTCC
    TGCTTACAGG TGGAGCGCAT CGAGGAGCTG GGTGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GCTGGAACGT TACGGGGCCC TGGGACCAGG ACAACTTCAT GGAGGTGCTG
    AAGGCAGTGG CAGGGACGTA TAGGGCCACC CCCTTCTTTA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGTT CCAACAGCAA TATTATCCAG GTGGACCAGT CTGGGCTCTT TCTACCCTCT
    CGAGATTACT ACCTAAACAG GACCGCCAAT GAGAAAGTGC TTACTGCCTA CCTGGACTAC
    ATGGAGGAGC TGGGGATGCT GCTGGGCGGA CAGCCAACCT CCACTCGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACGG TGCCCCAGGA CCAGCGGCGG
    GATGAGGAGA AGATCTACCA CAAGATGAGC ATCGCGGAGC TGCAGGCCCT GGCACCCTCC
    ATGGATTGGC TGGAGTTTCT GTCCTTCTTG CTGTCACCGC TGGAGCTGGG TGATTCTGAG
    CCTGTGGTGG TGTATGGGAC GGATTATTTG CAGCAGGTGT CGGAACTCAT CAACCGCACA
    GAGCCAAGTG TCCTGAACAA TTATCTGATC TGGAACCTGG TACAGAAGAC AACTTCAAGC
    CTGGACCACC GCTTTGAGTC TGCACAAGAA AAGCTGCTGG AGACCCTCTA TGGCACCAAG
    AAGTCCTGTA CACCGAGGTG GCAGACCTGC ATCTCCAACA CCGACGATGC TCTTGGCTTT
    GCTCTGGGCT CCCTCTTTGT GAAGGCCACA TTTGACCGGC AGAGCAAGGA AATTGCAGAG
    GGGATGATCA GCGAGATCCG AGCCGCCTTT GAGGAGGCTC TGGGACACTT GGTTTGGATG
    GACGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CCATCTATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA GCCCAAAGAG CTGGATGATG TTTATGATGG GTATGAAGTA
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ACTTCTCTGC TAAAGTGATG
    GCTGACCAGC TCCGCAAGCC TCCTAGCCGA GACCAGTGGA GCATGACCCC GCAGACAGTG
    AACGCCTACT ACCTTCCAAC CAAGAATGAA ATCGTCTTCC CTGCTGGCAT CCTGCAGGCC
    CCCTTCTACA CTTGCAACCA CCCCAAGGCC CTGAACTTCG GTGGCATCGG TGTGGTGATG
    GGCCACGAGT TGACACATGC CTTTGATGAC CAAGGGCGCG AGTATGACAA GGAAGGGAAT
    CTGCGGCCGT GGTGGCAGAA TGAGTCGTTG GCAGCCTTCC GGAACCACAC GGCCTGCATA
    GAGGAGCAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAAGC TCAACGGGCG CCAGACACTG
    GGGGAGAACA TTGCCGACAA TGGGGGGCTT AAGGCTGCCT ACAACGCTTA CAAAGCATGG
    CTAAGAAAGC ATGGGGAGGA GCAGCAGCTG CCAGCTGTGG GACTCACCAA CCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGGCTGTG GAGGAGGAAG CTCGGGGACA GAGTGGCTCG
    TCCAGGGGTA CCCAGGAAGA CAGAAAGTGG CAGACTCAGC AAGGTCTCCA GGTGTGGTGC
    TCGGTCCGCA CACCCGAGAG CTCTCACGAG GGGCTGGTGA CCGACCCCCA CAGCCCTGCC
    CGCTTCCGTG TGCTGGGCAC TCTCTCCAAC TCCCGTGACT TCCTGCGGCA CTTCGGCTGC
    CCTGTCGGCT CCCCCATGAA CTCAGGGCAG CTTTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87755
    Clone ID Related Accession (Same CDS sequence) XM_015472163.1 , XM_015472163.2
    Accession Version XM_015472163.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2388bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472163.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CAAACGCGCC 
    ACGCTGCGGG ATGAAGACGC TCCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC
    GTGGAGGCGG GATTCCGGAA GAGGACCAGT CGCCTCTTGG GGTTGCACAC CCAGCTGGAG
    CTGGTCTTGG CTGGTGTCTC TCTACTGCTG GCTGCCCTGC TTCTGGGTTG CTTGGTGGCC
    CTGGGGGTCC AGTACCACAG AGACCCATCC CATAGCACTT GCCTCACAGA GGCCTGCATT
    CGAGTGGCTG GAAAAATCCT GGAGTCCCTG GACCGTGGAG TGAGCCCCTG TGAGGACTTC
    TATCAGTTCT CCTGCGGAGG CTGGATTCGG AGAAACCCTC TACCTGATGG GCGTTCTCGC
    TGGAACACCT TCAACAGTCT CTGGGACCAG AATCAAGCCA TCCTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCCAG CAGTGAAGCT GAACGGAAGA CGCAGCGCTT CTACCTCTCC
    TGCTTACAGG TGGAGCGCAT CGAGGAGCTG GGTGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GCTGGAACGT TACGGGGCCC TGGGACCAGG ACAACTTCAT GGAGGTGCTG
    AAGGCAGTGG CAGGGACGTA TAGGGCCACC CCCTTCTTTA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGTT CCAACAGCAA TATTATCCAG GTGGACCAGT CTGGGCTCTT TCTACCCTCT
    CGAGATTACT ACCTAAACAG GACCGCCAAT GAGAAAGTGC TTACTGCCTA CCTGGACTAC
    ATGGAGGAGC TGGGGATGCT GCTGGGCGGA CAGCCAACCT CCACTCGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACGG TGCCCCAGGA CCAGCGGCGG
    GATGAGGAGA AGATCTACCA CAAGATGAGC ATCGCGGAGC TGCAGGCCCT GGCACCCTCC
    ATGGATTGGC TGGAGTTTCT GTCCTTCTTG CTGTCACCGC TGGAGCTGGG TGATTCTGAG
    CCTGTGGTGG TGTATGGGAC GGATTATTTG CAGCAGGTGT CGGAACTCAT CAACCGCACA
    GAGCCAAGTG TCCTGAACAA TTATCTGATC TGGAACCTGG TACAGAAGAC AACTTCAAGC
    CTGGACCACC GCTTTGAGTC TGCACAAGAA AAGCTGCTGG AGACCCTCTA TGGCACCAAG
    AAGTCCTGTA CACCGAGGTG GCAGACCTGC ATCTCCAACA CCGACGATGC TCTTGGCTTT
    GCTCTGGGCT CCCTCTTTGT GAAGGCCACA TTTGACCGGC AGAGCAAGGA AATTGCAGAG
    GGGATGATCA GCGAGATCCG AGCCGCCTTT GAGGAGGCTC TGGGACACTT GGTTTGGATG
    GACGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CCATCTATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA GCCCAAAGAG CTGGATGATG TTTATGATGG GTATGAAGTA
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ACTTCTCTGC TAAAGTGATG
    GCTGACCAGC TCCGCAAGCC TCCTAGCCGA GACCAGTGGA GCATGACCCC GCAGACAGTG
    AACGCCTACT ACCTTCCAAC CAAGAATGAA ATCGTCTTCC CTGCTGGCAT CCTGCAGGCC
    CCCTTCTACA CTTGCAACCA CCCCAAGGCC CTGAACTTCG GTGGCATCGG TGTGGTGATG
    GGCCACGAGT TGACACATGC CTTTGATGAC CAAGGGCGCG AGTATGACAA GGAAGGGAAT
    CTGCGGCCGT GGTGGCAGAA TGAGTCGTTG GCAGCCTTCC GGAACCACAC GGCCTGCATA
    GAGGAGCAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAAGC TCAACGGGCG CCAGACACTG
    GGGGAGAACA TTGCCGACAA TGGGGGGCTT AAGGCTGCCT ACAACGCTTA CAAAGCATGG
    CTAAGAAAGC ATGGGGAGGA GCAGCAGCTG CCAGCTGTGG GACTCACCAA CCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGGCTGTG GAGGAGGAAG CTCGGGGACA GAGTGGCTCG
    TCCAGGGGTA CCCAGGAAGA CAGAAAGTGG CAGACTCAGC AAGGTCTCCA GGTGTGGTGC
    TCGGTCCGCA CACCCGAGAG CTCTCACGAG GGGCTGGTGA CCGACCCCCA CAGCCCTGCC
    CGCTTCCGTG TGCTGGGCAC TCTCTCCAAC TCCCGTGACT TCCTGCGGCA CTTCGGCTGC
    CCTGTCGGCT CCCCCATGAA CTCAGGGCAG CTTTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327649.1
    CDS80..2467
    Translation

    Target ORF information:

    RefSeq Version XM_015472163.2
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472163.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACA TGGTGGAGTA CAAACGCGCC 
    ACGCTGCGGG ATGAAGACGC TCCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC
    GTGGAGGCGG GATTCCGGAA GAGGACCAGT CGCCTCTTGG GGTTGCACAC CCAGCTGGAG
    CTGGTCTTGG CTGGTGTCTC TCTACTGCTG GCTGCCCTGC TTCTGGGTTG CTTGGTGGCC
    CTGGGGGTCC AGTACCACAG AGACCCATCC CATAGCACTT GCCTCACAGA GGCCTGCATT
    CGAGTGGCTG GAAAAATCCT GGAGTCCCTG GACCGTGGAG TGAGCCCCTG TGAGGACTTC
    TATCAGTTCT CCTGCGGAGG CTGGATTCGG AGAAACCCTC TACCTGATGG GCGTTCTCGC
    TGGAACACCT TCAACAGTCT CTGGGACCAG AATCAAGCCA TCCTGAAGCA CCTGCTTGAA
    AACACCACCT TCAACTCCAG CAGTGAAGCT GAACGGAAGA CGCAGCGCTT CTACCTCTCC
    TGCTTACAGG TGGAGCGCAT CGAGGAGCTG GGTGCCCAGC CACTGCGAGA CCTCATTGAC
    AAGATTGGTG GCTGGAACGT TACGGGGCCC TGGGACCAGG ACAACTTCAT GGAGGTGCTG
    AAGGCAGTGG CAGGGACGTA TAGGGCCACC CCCTTCTTTA CTGTCTACGT CAGTGCCGAC
    TCTAAGAGTT CCAACAGCAA TATTATCCAG GTGGACCAGT CTGGGCTCTT TCTACCCTCT
    CGAGATTACT ACCTAAACAG GACCGCCAAT GAGAAAGTGC TTACTGCCTA CCTGGACTAC
    ATGGAGGAGC TGGGGATGCT GCTGGGCGGA CAGCCAACCT CCACTCGGGA GCAGATGCGG
    CAGGTGCTGG AGCTGGAGAT ACAACTGGCC AACATCACGG TGCCCCAGGA CCAGCGGCGG
    GATGAGGAGA AGATCTACCA CAAGATGAGC ATCGCGGAGC TGCAGGCCCT GGCACCCTCC
    ATGGATTGGC TGGAGTTTCT GTCCTTCTTG CTGTCACCGC TGGAGCTGGG TGATTCTGAG
    CCTGTGGTGG TGTATGGGAC GGATTATTTG CAGCAGGTGT CGGAACTCAT CAACCGCACA
    GAGCCAAGTG TCCTGAACAA TTATCTGATC TGGAACCTGG TACAGAAGAC AACTTCAAGC
    CTGGACCACC GCTTTGAGTC TGCACAAGAA AAGCTGCTGG AGACCCTCTA TGGCACCAAG
    AAGTCCTGTA CACCGAGGTG GCAGACCTGC ATCTCCAACA CCGACGATGC TCTTGGCTTT
    GCTCTGGGCT CCCTCTTTGT GAAGGCCACA TTTGACCGGC AGAGCAAGGA AATTGCAGAG
    GGGATGATCA GCGAGATCCG AGCCGCCTTT GAGGAGGCTC TGGGACACTT GGTTTGGATG
    GACGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CCATCTATGA TATGATTGGT
    TTCCCGGACT TCATCCTGGA GCCCAAAGAG CTGGATGATG TTTATGATGG GTATGAAGTA
    TCTGAAGATT CCTTCTTCCA GAACATGTTG AATTTGTACA ACTTCTCTGC TAAAGTGATG
    GCTGACCAGC TCCGCAAGCC TCCTAGCCGA GACCAGTGGA GCATGACCCC GCAGACAGTG
    AACGCCTACT ACCTTCCAAC CAAGAATGAA ATCGTCTTCC CTGCTGGCAT CCTGCAGGCC
    CCCTTCTACA CTTGCAACCA CCCCAAGGCC CTGAACTTCG GTGGCATCGG TGTGGTGATG
    GGCCACGAGT TGACACATGC CTTTGATGAC CAAGGGCGCG AGTATGACAA GGAAGGGAAT
    CTGCGGCCGT GGTGGCAGAA TGAGTCGTTG GCAGCCTTCC GGAACCACAC GGCCTGCATA
    GAGGAGCAGT ACAGCCAGTA CCAGGTCAAC GGGGAGAAGC TCAACGGGCG CCAGACACTG
    GGGGAGAACA TTGCCGACAA TGGGGGGCTT AAGGCTGCCT ACAACGCTTA CAAAGCATGG
    CTAAGAAAGC ATGGGGAGGA GCAGCAGCTG CCAGCTGTGG GACTCACCAA CCACCAGCTC
    TTCTTTGTGG GATTTGCCCA GGTGGCTGTG GAGGAGGAAG CTCGGGGACA GAGTGGCTCG
    TCCAGGGGTA CCCAGGAAGA CAGAAAGTGG CAGACTCAGC AAGGTCTCCA GGTGTGGTGC
    TCGGTCCGCA CACCCGAGAG CTCTCACGAG GGGCTGGTGA CCGACCCCCA CAGCCCTGCC
    CGCTTCCGTG TGCTGGGCAC TCTCTCCAAC TCCCGTGACT TCCTGCGGCA CTTCGGCTGC
    CCTGTCGGCT CCCCCATGAA CTCAGGGCAG CTTTGTGAGG TGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87756
    Clone ID Related Accession (Same CDS sequence) XM_015472166.1
    Accession Version XM_015472166.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGGAGT ACAAACGCGC CACGCTGCGG GATGAAGACG CTCCCGAGAC CCCCGTAGAG 
    GGCGGGGCCT CCCCGGACGC CGTGGAGGCG GGATTCCGGA AGAGGACCAG TCGCCTCTTG
    GGGTTGCACA CCCAGCTGGA GCTGGTCTTG GCTGGTGTCT CTCTACTGCT GGCTGCCCTG
    CTTCTGGGTT GCTTGGTGGC CCTGGGGGTC CAGTACCACA GAGACCCATC CCATAGCACT
    TGCCTCACAG AGGCCTGCAT TCGAGTGGCT GGAAAAATCC TGGAGTCCCT GGACCGTGGA
    GTGAGCCCCT GTGAGGACTT CTATCAGTTC TCCTGCGGAG GCTGGATTCG GAGAAACCCT
    CTACCTGATG GGCGTTCTCG CTGGAACACC TTCAACAGTC TCTGGGACCA GAATCAAGCC
    ATCCTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCCA GCAGTGAAGC TGAACGGAAG
    ACGCAGCGCT TCTACCTCTC CTGCTTACAG GTGGAGCGCA TCGAGGAGCT GGGTGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGCTGGAACG TTACGGGGCC CTGGGACCAG
    GACAACTTCA TGGAGGTGCT GAAGGCAGTG GCAGGGACGT ATAGGGCCAC CCCCTTCTTT
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGT TCCAACAGCA ATATTATCCA GGTGGACCAG
    TCTGGGCTCT TTCTACCCTC TCGAGATTAC TACCTAAACA GGACCGCCAA TGAGAAAGTG
    CTTACTGCCT ACCTGGACTA CATGGAGGAG CTGGGGATGC TGCTGGGCGG ACAGCCAACC
    TCCACTCGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACG
    GTGCCCCAGG ACCAGCGGCG GGATGAGGAG AAGATCTACC ACAAGATGAG CATCGCGGAG
    CTGCAGGCCC TGGCACCCTC CATGGATTGG CTGGAGTTTC TGTCCTTCTT GCTGTCACCG
    CTGGAGCTGG GTGATTCTGA GCCTGTGGTG GTGTATGGGA CGGATTATTT GCAGCAGGTG
    TCGGAACTCA TCAACCGCAC AGAGCCAAGT GTCCTGAACA ATTATCTGAT CTGGAACCTG
    GTACAGAAGA CAACTTCAAG CCTGGACCAC CGCTTTGAGT CTGCACAAGA AAAGCTGCTG
    GAGACCCTCT ATGGCACCAA GAAGTCCTGT ACACCGAGGT GGCAGACCTG CATCTCCAAC
    ACCGACGATG CTCTTGGCTT TGCTCTGGGC TCCCTCTTTG TGAAGGCCAC ATTTGACCGG
    CAGAGCAAGG AAATTGCAGA GGGGATGATC AGCGAGATCC GAGCCGCCTT TGAGGAGGCT
    CTGGGACACT TGGTTTGGAT GGACGAGAAG ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT
    GCCATCTATG ATATGATTGG TTTCCCGGAC TTCATCCTGG AGCCCAAAGA GCTGGATGAT
    GTTTATGATG GGTATGAAGT ATCTGAAGAT TCCTTCTTCC AGAACATGTT GAATTTGTAC
    AACTTCTCTG CTAAAGTGAT GGCTGACCAG CTCCGCAAGC CTCCTAGCCG AGACCAGTGG
    AGCATGACCC CGCAGACAGT GAACGCCTAC TACCTTCCAA CCAAGAATGA AATCGTCTTC
    CCTGCTGGCA TCCTGCAGGC CCCCTTCTAC ACTTGCAACC ACCCCAAGGC CCTGAACTTC
    GGTGGCATCG GTGTGGTGAT GGGCCACGAG TTGACACATG CCTTTGATGA CCAAGGGCGC
    GAGTATGACA AGGAAGGGAA TCTGCGGCCG TGGTGGCAGA ATGAGTCGTT GGCAGCCTTC
    CGGAACCACA CGGCCTGCAT AGAGGAGCAG TACAGCCAGT ACCAGGTCAA CGGGGAGAAG
    CTCAACGGGC GCCAGACACT GGGGGAGAAC ATTGCCGACA ATGGGGGGCT TAAGGCTGCC
    TACAACGCTT ACAAAGCATG GCTAAGAAAG CATGGGGAGG AGCAGCAGCT GCCAGCTGTG
    GGACTCACCA ACCACCAGCT CTTCTTTGTG GGATTTGCCC AGGTGGCTGT GGAGGAGGAA
    GCTCGGGGAC AGAGTGGCTC GTCCAGGGGT ACCCAGGAAG ACAGAAAGTG GCAGACTCAG
    CAAGGTCTCC AGGTGTGGTG CTCGGTCCGC ACACCCGAGA GCTCTCACGA GGGGCTGGTG
    ACCGACCCCC ACAGCCCTGC CCGCTTCCGT GTGCTGGGCA CTCTCTCCAA CTCCCGTGAC
    TTCCTGCGGC ACTTCGGCTG CCCTGTCGGC TCCCCCATGA ACTCAGGGCA GCTTTGTGAG
    GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327652.1
    CDS3866..6214
    Translation

    Target ORF information:

    RefSeq Version XM_015472166.1
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472166.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGTGGAGT ACAAACGCGC CACGCTGCGG GATGAAGACG CTCCCGAGAC CCCCGTAGAG 
    GGCGGGGCCT CCCCGGACGC CGTGGAGGCG GGATTCCGGA AGAGGACCAG TCGCCTCTTG
    GGGTTGCACA CCCAGCTGGA GCTGGTCTTG GCTGGTGTCT CTCTACTGCT GGCTGCCCTG
    CTTCTGGGTT GCTTGGTGGC CCTGGGGGTC CAGTACCACA GAGACCCATC CCATAGCACT
    TGCCTCACAG AGGCCTGCAT TCGAGTGGCT GGAAAAATCC TGGAGTCCCT GGACCGTGGA
    GTGAGCCCCT GTGAGGACTT CTATCAGTTC TCCTGCGGAG GCTGGATTCG GAGAAACCCT
    CTACCTGATG GGCGTTCTCG CTGGAACACC TTCAACAGTC TCTGGGACCA GAATCAAGCC
    ATCCTGAAGC ACCTGCTTGA AAACACCACC TTCAACTCCA GCAGTGAAGC TGAACGGAAG
    ACGCAGCGCT TCTACCTCTC CTGCTTACAG GTGGAGCGCA TCGAGGAGCT GGGTGCCCAG
    CCACTGCGAG ACCTCATTGA CAAGATTGGT GGCTGGAACG TTACGGGGCC CTGGGACCAG
    GACAACTTCA TGGAGGTGCT GAAGGCAGTG GCAGGGACGT ATAGGGCCAC CCCCTTCTTT
    ACTGTCTACG TCAGTGCCGA CTCTAAGAGT TCCAACAGCA ATATTATCCA GGTGGACCAG
    TCTGGGCTCT TTCTACCCTC TCGAGATTAC TACCTAAACA GGACCGCCAA TGAGAAAGTG
    CTTACTGCCT ACCTGGACTA CATGGAGGAG CTGGGGATGC TGCTGGGCGG ACAGCCAACC
    TCCACTCGGG AGCAGATGCG GCAGGTGCTG GAGCTGGAGA TACAACTGGC CAACATCACG
    GTGCCCCAGG ACCAGCGGCG GGATGAGGAG AAGATCTACC ACAAGATGAG CATCGCGGAG
    CTGCAGGCCC TGGCACCCTC CATGGATTGG CTGGAGTTTC TGTCCTTCTT GCTGTCACCG
    CTGGAGCTGG GTGATTCTGA GCCTGTGGTG GTGTATGGGA CGGATTATTT GCAGCAGGTG
    TCGGAACTCA TCAACCGCAC AGAGCCAAGT GTCCTGAACA ATTATCTGAT CTGGAACCTG
    GTACAGAAGA CAACTTCAAG CCTGGACCAC CGCTTTGAGT CTGCACAAGA AAAGCTGCTG
    GAGACCCTCT ATGGCACCAA GAAGTCCTGT ACACCGAGGT GGCAGACCTG CATCTCCAAC
    ACCGACGATG CTCTTGGCTT TGCTCTGGGC TCCCTCTTTG TGAAGGCCAC ATTTGACCGG
    CAGAGCAAGG AAATTGCAGA GGGGATGATC AGCGAGATCC GAGCCGCCTT TGAGGAGGCT
    CTGGGACACT TGGTTTGGAT GGACGAGAAG ACCCGCCAGG CAGCCAAGGA GAAAGCAGAT
    GCCATCTATG ATATGATTGG TTTCCCGGAC TTCATCCTGG AGCCCAAAGA GCTGGATGAT
    GTTTATGATG GGTATGAAGT ATCTGAAGAT TCCTTCTTCC AGAACATGTT GAATTTGTAC
    AACTTCTCTG CTAAAGTGAT GGCTGACCAG CTCCGCAAGC CTCCTAGCCG AGACCAGTGG
    AGCATGACCC CGCAGACAGT GAACGCCTAC TACCTTCCAA CCAAGAATGA AATCGTCTTC
    CCTGCTGGCA TCCTGCAGGC CCCCTTCTAC ACTTGCAACC ACCCCAAGGC CCTGAACTTC
    GGTGGCATCG GTGTGGTGAT GGGCCACGAG TTGACACATG CCTTTGATGA CCAAGGGCGC
    GAGTATGACA AGGAAGGGAA TCTGCGGCCG TGGTGGCAGA ATGAGTCGTT GGCAGCCTTC
    CGGAACCACA CGGCCTGCAT AGAGGAGCAG TACAGCCAGT ACCAGGTCAA CGGGGAGAAG
    CTCAACGGGC GCCAGACACT GGGGGAGAAC ATTGCCGACA ATGGGGGGCT TAAGGCTGCC
    TACAACGCTT ACAAAGCATG GCTAAGAAAG CATGGGGAGG AGCAGCAGCT GCCAGCTGTG
    GGACTCACCA ACCACCAGCT CTTCTTTGTG GGATTTGCCC AGGTGGCTGT GGAGGAGGAA
    GCTCGGGGAC AGAGTGGCTC GTCCAGGGGT ACCCAGGAAG ACAGAAAGTG GCAGACTCAG
    CAAGGTCTCC AGGTGTGGTG CTCGGTCCGC ACACCCGAGA GCTCTCACGA GGGGCTGGTG
    ACCGACCCCC ACAGCCCTGC CCGCTTCCGT GTGCTGGGCA CTCTCTCCAA CTCCCGTGAC
    TTCCTGCGGC ACTTCGGCTG CCCTGTCGGC TCCCCCATGA ACTCAGGGCA GCTTTGTGAG
    GTGTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87757
    Clone ID Related Accession (Same CDS sequence) XM_015472169.1 , XM_015472169.2
    Accession Version XM_015472169.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG CGGGATTCCG GAAGAGGACC 
    AGTCGCCTCT TGGGGTTGCA CACCCAGCTG GAGCTGGTCT TGGCTGGTGT CTCTCTACTG
    CTGGCTGCCC TGCTTCTGGG TTGCTTGGTG GCCCTGGGGG TCCAGTACCA CAGAGACCCA
    TCCCATAGCA CTTGCCTCAC AGAGGCCTGC ATTCGAGTGG CTGGAAAAAT CCTGGAGTCC
    CTGGACCGTG GAGTGAGCCC CTGTGAGGAC TTCTATCAGT TCTCCTGCGG AGGCTGGATT
    CGGAGAAACC CTCTACCTGA TGGGCGTTCT CGCTGGAACA CCTTCAACAG TCTCTGGGAC
    CAGAATCAAG CCATCCTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC CAGCAGTGAA
    GCTGAACGGA AGACGCAGCG CTTCTACCTC TCCTGCTTAC AGGTGGAGCG CATCGAGGAG
    CTGGGTGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGCTGGAA CGTTACGGGG
    CCCTGGGACC AGGACAACTT CATGGAGGTG CTGAAGGCAG TGGCAGGGAC GTATAGGGCC
    ACCCCCTTCT TTACTGTCTA CGTCAGTGCC GACTCTAAGA GTTCCAACAG CAATATTATC
    CAGGTGGACC AGTCTGGGCT CTTTCTACCC TCTCGAGATT ACTACCTAAA CAGGACCGCC
    AATGAGAAAG TGCTTACTGC CTACCTGGAC TACATGGAGG AGCTGGGGAT GCTGCTGGGC
    GGACAGCCAA CCTCCACTCG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CGGTGCCCCA GGACCAGCGG CGGGATGAGG AGAAGATCTA CCACAAGATG
    AGCATCGCGG AGCTGCAGGC CCTGGCACCC TCCATGGATT GGCTGGAGTT TCTGTCCTTC
    TTGCTGTCAC CGCTGGAGCT GGGTGATTCT GAGCCTGTGG TGGTGTATGG GACGGATTAT
    TTGCAGCAGG TGTCGGAACT CATCAACCGC ACAGAGCCAA GTGTCCTGAA CAATTATCTG
    ATCTGGAACC TGGTACAGAA GACAACTTCA AGCCTGGACC ACCGCTTTGA GTCTGCACAA
    GAAAAGCTGC TGGAGACCCT CTATGGCACC AAGAAGTCCT GTACACCGAG GTGGCAGACC
    TGCATCTCCA ACACCGACGA TGCTCTTGGC TTTGCTCTGG GCTCCCTCTT TGTGAAGGCC
    ACATTTGACC GGCAGAGCAA GGAAATTGCA GAGGGGATGA TCAGCGAGAT CCGAGCCGCC
    TTTGAGGAGG CTCTGGGACA CTTGGTTTGG ATGGACGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCCATCTA TGATATGATT GGTTTCCCGG ACTTCATCCT GGAGCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTATGAA GTATCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAACTTCTC TGCTAAAGTG ATGGCTGACC AGCTCCGCAA GCCTCCTAGC
    CGAGACCAGT GGAGCATGAC CCCGCAGACA GTGAACGCCT ACTACCTTCC AACCAAGAAT
    GAAATCGTCT TCCCTGCTGG CATCCTGCAG GCCCCCTTCT ACACTTGCAA CCACCCCAAG
    GCCCTGAACT TCGGTGGCAT CGGTGTGGTG ATGGGCCACG AGTTGACACA TGCCTTTGAT
    GACCAAGGGC GCGAGTATGA CAAGGAAGGG AATCTGCGGC CGTGGTGGCA GAATGAGTCG
    TTGGCAGCCT TCCGGAACCA CACGGCCTGC ATAGAGGAGC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA AGCTCAACGG GCGCCAGACA CTGGGGGAGA ACATTGCCGA CAATGGGGGG
    CTTAAGGCTG CCTACAACGC TTACAAAGCA TGGCTAAGAA AGCATGGGGA GGAGCAGCAG
    CTGCCAGCTG TGGGACTCAC CAACCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGGCT
    GTGGAGGAGG AAGCTCGGGG ACAGAGTGGC TCGTCCAGGG GTACCCAGGA AGACAGAAAG
    TGGCAGACTC AGCAAGGTCT CCAGGTGTGG TGCTCGGTCC GCACACCCGA GAGCTCTCAC
    GAGGGGCTGG TGACCGACCC CCACAGCCCT GCCCGCTTCC GTGTGCTGGG CACTCTCTCC
    AACTCCCGTG ACTTCCTGCG GCACTTCGGC TGCCCTGTCG GCTCCCCCAT GAACTCAGGG
    CAGCTTTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327655.1
    CDS74..2374
    Translation

    Target ORF information:

    RefSeq Version XM_015472169.1
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG CGGGATTCCG GAAGAGGACC 
    AGTCGCCTCT TGGGGTTGCA CACCCAGCTG GAGCTGGTCT TGGCTGGTGT CTCTCTACTG
    CTGGCTGCCC TGCTTCTGGG TTGCTTGGTG GCCCTGGGGG TCCAGTACCA CAGAGACCCA
    TCCCATAGCA CTTGCCTCAC AGAGGCCTGC ATTCGAGTGG CTGGAAAAAT CCTGGAGTCC
    CTGGACCGTG GAGTGAGCCC CTGTGAGGAC TTCTATCAGT TCTCCTGCGG AGGCTGGATT
    CGGAGAAACC CTCTACCTGA TGGGCGTTCT CGCTGGAACA CCTTCAACAG TCTCTGGGAC
    CAGAATCAAG CCATCCTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC CAGCAGTGAA
    GCTGAACGGA AGACGCAGCG CTTCTACCTC TCCTGCTTAC AGGTGGAGCG CATCGAGGAG
    CTGGGTGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGCTGGAA CGTTACGGGG
    CCCTGGGACC AGGACAACTT CATGGAGGTG CTGAAGGCAG TGGCAGGGAC GTATAGGGCC
    ACCCCCTTCT TTACTGTCTA CGTCAGTGCC GACTCTAAGA GTTCCAACAG CAATATTATC
    CAGGTGGACC AGTCTGGGCT CTTTCTACCC TCTCGAGATT ACTACCTAAA CAGGACCGCC
    AATGAGAAAG TGCTTACTGC CTACCTGGAC TACATGGAGG AGCTGGGGAT GCTGCTGGGC
    GGACAGCCAA CCTCCACTCG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CGGTGCCCCA GGACCAGCGG CGGGATGAGG AGAAGATCTA CCACAAGATG
    AGCATCGCGG AGCTGCAGGC CCTGGCACCC TCCATGGATT GGCTGGAGTT TCTGTCCTTC
    TTGCTGTCAC CGCTGGAGCT GGGTGATTCT GAGCCTGTGG TGGTGTATGG GACGGATTAT
    TTGCAGCAGG TGTCGGAACT CATCAACCGC ACAGAGCCAA GTGTCCTGAA CAATTATCTG
    ATCTGGAACC TGGTACAGAA GACAACTTCA AGCCTGGACC ACCGCTTTGA GTCTGCACAA
    GAAAAGCTGC TGGAGACCCT CTATGGCACC AAGAAGTCCT GTACACCGAG GTGGCAGACC
    TGCATCTCCA ACACCGACGA TGCTCTTGGC TTTGCTCTGG GCTCCCTCTT TGTGAAGGCC
    ACATTTGACC GGCAGAGCAA GGAAATTGCA GAGGGGATGA TCAGCGAGAT CCGAGCCGCC
    TTTGAGGAGG CTCTGGGACA CTTGGTTTGG ATGGACGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCCATCTA TGATATGATT GGTTTCCCGG ACTTCATCCT GGAGCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTATGAA GTATCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAACTTCTC TGCTAAAGTG ATGGCTGACC AGCTCCGCAA GCCTCCTAGC
    CGAGACCAGT GGAGCATGAC CCCGCAGACA GTGAACGCCT ACTACCTTCC AACCAAGAAT
    GAAATCGTCT TCCCTGCTGG CATCCTGCAG GCCCCCTTCT ACACTTGCAA CCACCCCAAG
    GCCCTGAACT TCGGTGGCAT CGGTGTGGTG ATGGGCCACG AGTTGACACA TGCCTTTGAT
    GACCAAGGGC GCGAGTATGA CAAGGAAGGG AATCTGCGGC CGTGGTGGCA GAATGAGTCG
    TTGGCAGCCT TCCGGAACCA CACGGCCTGC ATAGAGGAGC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA AGCTCAACGG GCGCCAGACA CTGGGGGAGA ACATTGCCGA CAATGGGGGG
    CTTAAGGCTG CCTACAACGC TTACAAAGCA TGGCTAAGAA AGCATGGGGA GGAGCAGCAG
    CTGCCAGCTG TGGGACTCAC CAACCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGGCT
    GTGGAGGAGG AAGCTCGGGG ACAGAGTGGC TCGTCCAGGG GTACCCAGGA AGACAGAAAG
    TGGCAGACTC AGCAAGGTCT CCAGGTGTGG TGCTCGGTCC GCACACCCGA GAGCTCTCAC
    GAGGGGCTGG TGACCGACCC CCACAGCCCT GCCCGCTTCC GTGTGCTGGG CACTCTCTCC
    AACTCCCGTG ACTTCCTGCG GCACTTCGGC TGCCCTGTCG GCTCCCCCAT GAACTCAGGG
    CAGCTTTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87757
    Clone ID Related Accession (Same CDS sequence) XM_015472169.1 , XM_015472169.2
    Accession Version XM_015472169.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product endothelin-converting enzyme 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472169.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG CGGGATTCCG GAAGAGGACC 
    AGTCGCCTCT TGGGGTTGCA CACCCAGCTG GAGCTGGTCT TGGCTGGTGT CTCTCTACTG
    CTGGCTGCCC TGCTTCTGGG TTGCTTGGTG GCCCTGGGGG TCCAGTACCA CAGAGACCCA
    TCCCATAGCA CTTGCCTCAC AGAGGCCTGC ATTCGAGTGG CTGGAAAAAT CCTGGAGTCC
    CTGGACCGTG GAGTGAGCCC CTGTGAGGAC TTCTATCAGT TCTCCTGCGG AGGCTGGATT
    CGGAGAAACC CTCTACCTGA TGGGCGTTCT CGCTGGAACA CCTTCAACAG TCTCTGGGAC
    CAGAATCAAG CCATCCTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC CAGCAGTGAA
    GCTGAACGGA AGACGCAGCG CTTCTACCTC TCCTGCTTAC AGGTGGAGCG CATCGAGGAG
    CTGGGTGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGCTGGAA CGTTACGGGG
    CCCTGGGACC AGGACAACTT CATGGAGGTG CTGAAGGCAG TGGCAGGGAC GTATAGGGCC
    ACCCCCTTCT TTACTGTCTA CGTCAGTGCC GACTCTAAGA GTTCCAACAG CAATATTATC
    CAGGTGGACC AGTCTGGGCT CTTTCTACCC TCTCGAGATT ACTACCTAAA CAGGACCGCC
    AATGAGAAAG TGCTTACTGC CTACCTGGAC TACATGGAGG AGCTGGGGAT GCTGCTGGGC
    GGACAGCCAA CCTCCACTCG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CGGTGCCCCA GGACCAGCGG CGGGATGAGG AGAAGATCTA CCACAAGATG
    AGCATCGCGG AGCTGCAGGC CCTGGCACCC TCCATGGATT GGCTGGAGTT TCTGTCCTTC
    TTGCTGTCAC CGCTGGAGCT GGGTGATTCT GAGCCTGTGG TGGTGTATGG GACGGATTAT
    TTGCAGCAGG TGTCGGAACT CATCAACCGC ACAGAGCCAA GTGTCCTGAA CAATTATCTG
    ATCTGGAACC TGGTACAGAA GACAACTTCA AGCCTGGACC ACCGCTTTGA GTCTGCACAA
    GAAAAGCTGC TGGAGACCCT CTATGGCACC AAGAAGTCCT GTACACCGAG GTGGCAGACC
    TGCATCTCCA ACACCGACGA TGCTCTTGGC TTTGCTCTGG GCTCCCTCTT TGTGAAGGCC
    ACATTTGACC GGCAGAGCAA GGAAATTGCA GAGGGGATGA TCAGCGAGAT CCGAGCCGCC
    TTTGAGGAGG CTCTGGGACA CTTGGTTTGG ATGGACGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCCATCTA TGATATGATT GGTTTCCCGG ACTTCATCCT GGAGCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTATGAA GTATCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAACTTCTC TGCTAAAGTG ATGGCTGACC AGCTCCGCAA GCCTCCTAGC
    CGAGACCAGT GGAGCATGAC CCCGCAGACA GTGAACGCCT ACTACCTTCC AACCAAGAAT
    GAAATCGTCT TCCCTGCTGG CATCCTGCAG GCCCCCTTCT ACACTTGCAA CCACCCCAAG
    GCCCTGAACT TCGGTGGCAT CGGTGTGGTG ATGGGCCACG AGTTGACACA TGCCTTTGAT
    GACCAAGGGC GCGAGTATGA CAAGGAAGGG AATCTGCGGC CGTGGTGGCA GAATGAGTCG
    TTGGCAGCCT TCCGGAACCA CACGGCCTGC ATAGAGGAGC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA AGCTCAACGG GCGCCAGACA CTGGGGGAGA ACATTGCCGA CAATGGGGGG
    CTTAAGGCTG CCTACAACGC TTACAAAGCA TGGCTAAGAA AGCATGGGGA GGAGCAGCAG
    CTGCCAGCTG TGGGACTCAC CAACCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGGCT
    GTGGAGGAGG AAGCTCGGGG ACAGAGTGGC TCGTCCAGGG GTACCCAGGA AGACAGAAAG
    TGGCAGACTC AGCAAGGTCT CCAGGTGTGG TGCTCGGTCC GCACACCCGA GAGCTCTCAC
    GAGGGGCTGG TGACCGACCC CCACAGCCCT GCCCGCTTCC GTGTGCTGGG CACTCTCTCC
    AACTCCCGTG ACTTCCTGCG GCACTTCGGC TGCCCTGTCG GCTCCCCCAT GAACTCAGGG
    CAGCTTTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327655.1
    CDS80..2380
    Translation

    Target ORF information:

    RefSeq Version XM_015472169.2
    Organism Bos taurus(cattle)
    Definition Bos taurus endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472169.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGCGTCG CGCTGCAGGA GCTGGGCGGC GGCGGCAACG CGGGATTCCG GAAGAGGACC 
    AGTCGCCTCT TGGGGTTGCA CACCCAGCTG GAGCTGGTCT TGGCTGGTGT CTCTCTACTG
    CTGGCTGCCC TGCTTCTGGG TTGCTTGGTG GCCCTGGGGG TCCAGTACCA CAGAGACCCA
    TCCCATAGCA CTTGCCTCAC AGAGGCCTGC ATTCGAGTGG CTGGAAAAAT CCTGGAGTCC
    CTGGACCGTG GAGTGAGCCC CTGTGAGGAC TTCTATCAGT TCTCCTGCGG AGGCTGGATT
    CGGAGAAACC CTCTACCTGA TGGGCGTTCT CGCTGGAACA CCTTCAACAG TCTCTGGGAC
    CAGAATCAAG CCATCCTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC CAGCAGTGAA
    GCTGAACGGA AGACGCAGCG CTTCTACCTC TCCTGCTTAC AGGTGGAGCG CATCGAGGAG
    CTGGGTGCCC AGCCACTGCG AGACCTCATT GACAAGATTG GTGGCTGGAA CGTTACGGGG
    CCCTGGGACC AGGACAACTT CATGGAGGTG CTGAAGGCAG TGGCAGGGAC GTATAGGGCC
    ACCCCCTTCT TTACTGTCTA CGTCAGTGCC GACTCTAAGA GTTCCAACAG CAATATTATC
    CAGGTGGACC AGTCTGGGCT CTTTCTACCC TCTCGAGATT ACTACCTAAA CAGGACCGCC
    AATGAGAAAG TGCTTACTGC CTACCTGGAC TACATGGAGG AGCTGGGGAT GCTGCTGGGC
    GGACAGCCAA CCTCCACTCG GGAGCAGATG CGGCAGGTGC TGGAGCTGGA GATACAACTG
    GCCAACATCA CGGTGCCCCA GGACCAGCGG CGGGATGAGG AGAAGATCTA CCACAAGATG
    AGCATCGCGG AGCTGCAGGC CCTGGCACCC TCCATGGATT GGCTGGAGTT TCTGTCCTTC
    TTGCTGTCAC CGCTGGAGCT GGGTGATTCT GAGCCTGTGG TGGTGTATGG GACGGATTAT
    TTGCAGCAGG TGTCGGAACT CATCAACCGC ACAGAGCCAA GTGTCCTGAA CAATTATCTG
    ATCTGGAACC TGGTACAGAA GACAACTTCA AGCCTGGACC ACCGCTTTGA GTCTGCACAA
    GAAAAGCTGC TGGAGACCCT CTATGGCACC AAGAAGTCCT GTACACCGAG GTGGCAGACC
    TGCATCTCCA ACACCGACGA TGCTCTTGGC TTTGCTCTGG GCTCCCTCTT TGTGAAGGCC
    ACATTTGACC GGCAGAGCAA GGAAATTGCA GAGGGGATGA TCAGCGAGAT CCGAGCCGCC
    TTTGAGGAGG CTCTGGGACA CTTGGTTTGG ATGGACGAGA AGACCCGCCA GGCAGCCAAG
    GAGAAAGCAG ATGCCATCTA TGATATGATT GGTTTCCCGG ACTTCATCCT GGAGCCCAAA
    GAGCTGGATG ATGTTTATGA TGGGTATGAA GTATCTGAAG ATTCCTTCTT CCAGAACATG
    TTGAATTTGT ACAACTTCTC TGCTAAAGTG ATGGCTGACC AGCTCCGCAA GCCTCCTAGC
    CGAGACCAGT GGAGCATGAC CCCGCAGACA GTGAACGCCT ACTACCTTCC AACCAAGAAT
    GAAATCGTCT TCCCTGCTGG CATCCTGCAG GCCCCCTTCT ACACTTGCAA CCACCCCAAG
    GCCCTGAACT TCGGTGGCAT CGGTGTGGTG ATGGGCCACG AGTTGACACA TGCCTTTGAT
    GACCAAGGGC GCGAGTATGA CAAGGAAGGG AATCTGCGGC CGTGGTGGCA GAATGAGTCG
    TTGGCAGCCT TCCGGAACCA CACGGCCTGC ATAGAGGAGC AGTACAGCCA GTACCAGGTC
    AACGGGGAGA AGCTCAACGG GCGCCAGACA CTGGGGGAGA ACATTGCCGA CAATGGGGGG
    CTTAAGGCTG CCTACAACGC TTACAAAGCA TGGCTAAGAA AGCATGGGGA GGAGCAGCAG
    CTGCCAGCTG TGGGACTCAC CAACCACCAG CTCTTCTTTG TGGGATTTGC CCAGGTGGCT
    GTGGAGGAGG AAGCTCGGGG ACAGAGTGGC TCGTCCAGGG GTACCCAGGA AGACAGAAAG
    TGGCAGACTC AGCAAGGTCT CCAGGTGTGG TGCTCGGTCC GCACACCCGA GAGCTCTCAC
    GAGGGGCTGG TGACCGACCC CCACAGCCCT GCCCGCTTCC GTGTGCTGGG CACTCTCTCC
    AACTCCCGTG ACTTCCTGCG GCACTTCGGC TGCCCTGTCG GCTCCCCCAT GAACTCAGGG
    CAGCTTTGTG AGGTGTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.