CASR cDNA ORF clone, Bos taurus(cattle)

The following CASR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa00026 NM_174002.3
Latest version!
Bos taurus calcium sensing receptor (CASR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa00026 XM_005201385.4
Latest version!
Bos taurus calcium sensing receptor (CASR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa00026 XM_010801164.3
Latest version!
Bos taurus calcium sensing receptor (CASR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa15660 XM_005201385.3
Latest version!
Bos taurus calcium-sensing receptor (CASR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa15660 XM_010801164.2
Latest version!
Bos taurus calcium-sensing receptor (CASR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa00026
    Clone ID Related Accession (Same CDS sequence) XM_010801164.3 , XM_005201385.4 , NM_174002.3
    Accession Version NM_174002.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Bos taurus(cattle)
    Product extracellular calcium-sensing receptor precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from S67307.1. On Feb 21, 2013 this sequence version replaced NM_174002.2. ##Evidence-Data-START## Transcript exon combination :: S67307.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145420, SAMN03145448 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTCCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776427.1
    CDS511..3768
    Translation

    Target ORF information:

    RefSeq Version NM_174002.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium sensing receptor (CASR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174002.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTCCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa00026
    Clone ID Related Accession (Same CDS sequence) XM_010801164.3 , XM_005201385.4 , NM_174002.3
    Accession Version XM_010801164.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801164.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTCCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799466.2
    CDS463..3720
    Translation

    Target ORF information:

    RefSeq Version XM_010801164.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium sensing receptor (CASR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801164.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTCCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa00026
    Clone ID Related Accession (Same CDS sequence) XM_010801164.3 , XM_005201385.4 , NM_174002.3
    Accession Version XM_005201385.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201385.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTCCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201442.2
    CDS891..4148
    Translation

    Target ORF information:

    RefSeq Version XM_005201385.4
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium sensing receptor (CASR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201385.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTCCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15660
    Clone ID Related Accession (Same CDS sequence) XM_005201385.3 , XM_010801164.2
    Accession Version XM_005201385.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTTCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201442.1
    CDS397..3654
    Translation

    Target ORF information:

    RefSeq Version XM_005201385.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium-sensing receptor (CASR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201385.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTTCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa15660
    Clone ID Related Accession (Same CDS sequence) XM_005201385.3 , XM_010801164.2
    Accession Version XM_010801164.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3258bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product extracellular calcium-sensing receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103811.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTTCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799466.1
    CDS539..3796
    Translation

    Target ORF information:

    RefSeq Version XM_010801164.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium-sensing receptor (CASR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801164.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACTTT ATAGCTGCTG TTGGATCCTC TTGGCTTTTT CTACCTGGTG CACTTCCGCC 
    TATGGGCCTG ACCAGCGAGC CCAAAAGAAA GGGGACATTA TCCTCGGGGG GCTCTTTCCT
    ATTCATTTTG GGGTTGCAGT GAAAGATCAG GATCTAAAGT CGAGGCCGGA GTCCGTGGAG
    TGTATCAGGT ATAATTTCCG AGGATTTCGC TGGTTACAAG CTATGATATT TGCCATAGAG
    GAAATAAACA GCAGTCCAGC CCTTCTTCCC AACATGACCC TGGGATACAG GATATTCGAC
    ACTTGTAACA CCGTCTCTAA AGCCTTGGAG GCCACCCTGA GTTTTGTGGC CCAGAACAAA
    ATTGACTCTT TGAACCTTGA TGAGTTCTGC AACTGCTCAG AGCACATCCC CTCTACCATC
    GCAGTGGTGG GAGCTACTGG CTCGGGCATC TCCACAGCAG TGGCCAACCT GCTGGGGCTC
    TTCTACATCC CCCAGGTCAG CTATGCCTCC TCCAGCAGAC TCCTCAGCAA CAAGAATCAA
    TTCAAGTCCT TCCTCCGCAC CATACCCAAT GATGAACACC AGGCCACGGC CATGGCTGAC
    ATCATCGAGT ACTTCCGCTG GAACTGGGTG GGCACAATTG CAGCTGACGA TGACTATGGC
    CGGCCAGGGA TCGAGAAGTT TCGAGAGGAA GCTGAGGAGA GGGACATCTG CATCGACTTC
    AGCGAGCTCA TCTCCCAATA CTCTGATGAG GAAAAGATCC AGCAGGTGGT GGAGGTGATC
    CAGAATTCCA CCGCCAAAGT CATTGTCGTC TTCTCCAGCG GCCCAGACCT GGAACCCCTC
    ATCAAAGAGA TCGTCCGGCG CAATATCACA GGCAGGATCT GGCTGGCCAG CGAGGCCTGG
    GCCAGCTCTT CCCTGATTGC TATGCCCGAG TATTTCCATG TGGTCGGAGG CACCATTGGG
    TTTGGTTTGA AAGCTGGGCA GATCCCAGGC TTCCGGGAAT TCCTGCAGAA AGTCCACCCC
    AGGAAGTCTG TCCACAATGG TTTTGCCAAG GAGTTTTGGG AAGAAACATT TAACTGCCAC
    CTGCAAGAGG GTGCTAAAGG CCCATTACCG GTGGACACCT TCCTGAGAGG TCACGAAGAA
    GGAGGTGCCA GGTTAAGCAA CAGTCCCACT GCCTTCCGAC CTCTGTGCAC TGGGGAGGAG
    AACATCAGCA GTGTCGAGAC TCCTTACATG GATTATACAC ATTTACGGAT ATCCTACAAC
    GTCTACTTAG CCGTCTACTC CATTGCTCAT GCCCTACAAG ATATATACAC CTGCATACCT
    GGGAGAGGGC TCTTCACCAA CGGTTCCTGC GCAGATATCA AGAAGGTTGA AGCTTGGCAG
    GTCCTGAAAC ACCTGCGGCA CCTAAATTTT ACCAGCAATA TGGGGGAGCA AGTAACTTTC
    GATGAATGTG GAGACCTGGC AGGGAACTAT TCCATCATCA ACTGGCACCT CTCCCCAGAG
    GACGGCTCCA TAGTGTTTAA GGAAGTTGGA TATTACAATG TCTATGCCAA GAAAGGAGAG
    AGACTCTTCA TCAATGATGA AAAAATTCTG TGGAGTGGAT TCTCAAGGGA GGTGCCTTTC
    TCCAACTGCA GTCGAGACTG CCTGGCAGGG ACCAGGAAAG GAATCATTGA GGGGGAGCCC
    ACCTGCTGCT TTGAGTGTGT GGAATGTCCT GATGGGGAGT ACAGCGACGA GACAGATGCA
    AGTGCCTGTG ATAAGTGCCC TGATGACTTC TGGTCCAATG AGAACCACAC TTCCTGCATC
    GCCAAGGAGA TCGAGTTTCT GTCGTGGACC GAGCCCTTCG GGATCGCACT CACGCTCTTT
    GCTGTGCTGG GCATTTTCCT CACAGCCTTC GTGCTGGGCG TCTTCATCAA GTTCCGCAAC
    ACGCCCATCG TCAAGGCCAC CAACCGGGAG CTCTCCTATC TCCTTCTCTT CTCCCTGCTC
    TGCTGCTTCT CCAGCTCCCT GTTCTTCATC GGGGAGCCCC AGGACTGGAC GTGCCGCCTG
    CGCCAGCCGG CCTTTGGCAT CAGCTTCGTG CTCTGCATCT CGTGCATCCT GGTGAAAACC
    AATCGGGTCC TCCTGGTGTT TGAGGCCAAG ATTCCCACCA GCTTCCACCG GAAGTGGTGG
    GGGCTCAACC TGCAGTTCCT GCTGGTCTTC CTCTGCACCT TCATGCAGAT TGTCATCTGT
    GCCATTTGGC TCAATACAGC GCCCCCCTCG AGCTACCGCA ACCACGAGCT GGAGGACGAG
    ATCATCTTCA TCACCTGCCA CGAGGGCTCG CTCATGGCGC TGGGCTTCCT GATCGGCTAC
    ACCTGCTTGC TGGCCGCCAT CTGCTTCTTC TTCGCCTTCA AGTCCCGGAA GCTGCCAGAG
    AACTTCAATG AAGCCAAGTT CATCACCTTC AGCATGCTCA TCTTCTTCAT CGTCTGGATC
    TCTTTCATCC CCGCCTACGC CAGCACTTAC GGCAAGTTCG TCTCTGCCGT GGAGGTGATC
    GCCATCCTGG CGGCCAGCTT TGGCTTGCTG GCCTGTATCT TCTTCAACAA GGTCTACATC
    ATCCTCTTCA AGCCTTCCCG GAACACCATC GAGGAGGTGC GCTGCAGCAC CGCGGCACAC
    GCCTTCAAGG TGGCCGCCCG AGCCACGCTG CGCCGCAGCA ACGTCTCCCG CCAGCGGTCC
    AGCAGCCTAG GGGGCTCCAC GGGATCCACC CCCTCCTCCT CCATCAGCAG CAAGAGCAAC
    AGCGAGGACC CGTTCCCTCA GCAGCAGCCG AAGAGGCAGA AGCAGCCGCA GCCGCTGGCC
    CTGAGCCCGC ACAACGCGCA GCAGCCACAG CCGCGGCCAC CCTCGACCCC ACAGCCGCAG
    CCACAGTCGC AGCAGCCGCC CCGATGCAAG CAGAAGGTCA TCTTCGGCAG CGGCACCGTC
    ACCTTCTCGC TGAGCTTTGA CGAGCCTCAG AAGACCGCCG TGGCTCACAG GAATTTCACG
    CACCAGACCT CCCTGGAGGC CCAGAAAAAC AATGACGCCC TGACCAAACA CCAGGCGTTG
    CTCCCGCTGC AGTGCGGAGA GACGGACTCA GAATTGACCT CCCAGGAGAC AGGCCTGCAG
    GGCCCTGTGG GTGAGGACCA CCAGCTAGAG ATGGAGGACC CCGAAGAGAT GTCCCCGGCA
    CTTGTAGTGT CTAATTCCCG GAGCTTTGTC ATCAGTGGCG GAGGCAGCAC TGTTACGGAA
    AACATGCTGC GTTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.