RNASE1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNASE1
Entrez Gene ID	280930
Full Name	ribonuclease, RNase A family, 1 (pancreatic)
Synonyms	RAC1,SRN
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_181810.1	NP_861526.1	seminal ribonuclease precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Stability strengths and weaknesses in protein structures detected by statistical potentials: Application to bovine seminal ribonuclease.
De Laet M, Gilis D, Rooman M
Proteins84(1)143-58(2016 Jan)

Comparative genomic analysis of eutherian ribonuclease A genes.
Premzl M
Molecular genetics and genomics : MGG289(2)161-7(2014 Apr)

Mechanism of 3D domain swapping in bovine seminal ribonuclease.
Spadaccini R, Ercole C, Graziano G, Wechselberger R, Boelens R, Picone D
The FEBS journal281(3)842-50(2014 Feb)

The multiple forms of bovine seminal ribonuclease: structure and stability of a C-terminal swapped dimer.
Sica F, Pica A, Merlino A, Russo Krauss I, Ercole C, Picone D
FEBS letters587(23)3755-62(2013 Nov)

Double domain swapping in bovine seminal RNase: formation of distinct N- and C-swapped tetramers and multimers with increasing biological activities.
Gotte G, Mahmoud Helmy A, Ercole C, Spadaccini R, Laurents DV, Donadelli M, Picone D
PloS one7(10)e46804(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the regions involved in 3D swapping, the study observed an accumulation of weaknesses in the monomer, which disappear in the dimer and especially in the swapped dimer.
Title: Stability strengths and weaknesses in protein structures detected by statistical potentials: Application to bovine seminal ribonuclease.

Data suggest that dimerization of seminal ribonuclease (SRN) facilitates three-dimensional domain swapping; both swapped and un-swapped dimers of SRN exhibit increased flexibility as compared to monomeric subunit SRN.
Title: Mechanism of 3D domain swapping in bovine seminal ribonuclease.

A report of the first clear experimental evidence on the formation of a C-terminal swapped dimer of the monomeric BS-RNase derivatives.
Title: The multiple forms of bovine seminal ribonuclease: structure and stability of a C-terminal swapped dimer.

Data indicate that BS-RNase oligomers are more enzymatically active than the native dimer.
Title: Double domain swapping in bovine seminal RNase: formation of distinct N- and C-swapped tetramers and multimers with increasing biological activities.

The domain swapping in BS-RNase must be influenced by the distinct features of the dimers, suggesting a prominent role for the interchain disulfide bridges.
Title: NMR studies on structure and dynamics of the monomeric derivative of BS-RNase: new insights for 3D domain swapping.

The following RNASE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNASE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01499	NM_181810.1 Latest version!	Bos taurus ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNASE1 ( NM_181810.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_861526.1 Bos taurus ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), mRNA.

Price & Availability↑

CloneID	OBa01499
Clone ID Related Accession (Same CDS sequence)	NM_181810.1
Accession Version	NM_181810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	seminal ribonuclease precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ000518.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DY156885.1, DY151395.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTCTGA AGTCTCTAGT CGTGTTGCCA CTGCTGGTCC TGGTGCTGCT GCTGGTGCGG 
GTCCAGCCTT CCCTGGGCAA GGAATCTGCA GCTGCCAAGT TCGAGCGGCA GCACATGGAC 
TCTGGCAACT CCCCCAGCAG CAGCTCCAAC TACTGCAACC TGATGATGTG CTGCCGGAAG 
ATGACCCAGG GGAAATGCAA GCCAGTGAAC ACCTTTGTGC ATGAGTCCCT GGCCGATGTT 
AAGGCCGTGT GCTCCCAGAA GAAAGTCACT TGCAAGAATG GGCAGACCAA CTGCTACCAG 
AGCAAATCCA CCATGCGCAT CACAGACTGC CGCGAGACTG GCAGCTCCAA GTACCCCAAC 
TGCGCCTACA AGACCACCCA GGTGGAGAAA CACATCATAG TGGCTTGTGG CGGTAAACCG 
TCCGTGCCAG TCCACTTCGA TGCTTCAGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_861526.1
CDS	65..517
Translation	MALKSLVVLPLLVLVLLLVRVQPSLGKESAAAKFERQHMDSGNSPSSSSNYCNLMMCCRKMTQGKCKPVNTFVHESLADVKAVCSQKKVTCKNGQTNCYQSKSTMRITDCRETGSSKYPNCAYKTTQVEKHIIVACGGKPSVPVHFDASV

Target ORF information:

RefSeq Version	NM_181810.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ribonuclease, RNase A family, 1 (pancreatic) (RNASE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181810.1

ORF Insert Sequence:

ATGGCTCTGA AGTCTCTAGT CGTGTTGCCA CTGCTGGTCC TGGTGCTGCT GCTGGTGCGG 
GTCCAGCCTT CCCTGGGCAA GGAATCTGCA GCTGCCAAGT TCGAGCGGCA GCACATGGAC 
TCTGGCAACT CCCCCAGCAG CAGCTCCAAC TACTGCAACC TGATGATGTG CTGCCGGAAG 
ATGACCCAGG GGAAATGCAA GCCAGTGAAC ACCTTTGTGC ATGAGTCCCT GGCCGATGTT 
AAGGCCGTGT GCTCCCAGAA GAAAGTCACT TGCAAGAATG GGCAGACCAA CTGCTACCAG 
AGCAAATCCA CCATGCGCAT CACAGACTGC CGCGAGACTG GCAGCTCCAA GTACCCCAAC 
TGCGCCTACA AGACCACCCA GGTGGAGAAA CACATCATAG TGGCTTGTGG CGGTAAACCG 
TCCGTGCCAG TCCACTTCGA TGCTTCAGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stability strengths and weaknesses in protein structures detected by statistical potentials: Application to bovine seminal ribonuclease.
Proteins84(1)143-58(2016 Jan)
De Laet M,Gilis D,Rooman M

Comparative genomic analysis of eutherian ribonuclease A genes.
Molecular genetics and genomics : MGG289(2)161-7(2014 Apr)
Premzl M

Mechanism of 3D domain swapping in bovine seminal ribonuclease.
The FEBS journal281(3)842-50(2014 Feb)
Spadaccini R,Ercole C,Graziano G,Wechselberger R,Boelens R,Picone D

The multiple forms of bovine seminal ribonuclease: structure and stability of a C-terminal swapped dimer.
FEBS letters587(23)3755-62(2013 Nov)
Sica F,Pica A,Merlino A,Russo Krauss I,Ercole C,Picone D

Double domain swapping in bovine seminal RNase: formation of distinct N- and C-swapped tetramers and multimers with increasing biological activities.
PloS one7(10)e46804(2012)
Gotte G,Mahmoud Helmy A,Ercole C,Spadaccini R,Laurents DV,Donadelli M,Picone D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

RNASE1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報