OXT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	OXT
Entrez Gene ID	280888
Full Name	oxytocin/neurophysin I prepropeptide
Synonyms	OT-NPI,OTNPI
General protein information	Preferred Names oxytocin/neurophysin I prepropeptide Names oxytocin-neurophysin 1 oxytocin, prepro- (neurophysin I) oxytocin, prepropeptide prepro-oxytocin
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 13 Map Location: 13q21-q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_176855.1	NP_789825.1	oxytocin-neurophysin 1 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	OXT	NP_000906.1
	OXT	XP_001115045.1
Canis lupus familiaris (dog)	OXT	XP_003639897.2
Bos taurus (cattle)	OXT	NP_789825.1
Gallus gallus (chicken)	OXT	XP_001231492.3
Pan troglodytes (chimpanzee)	OXT	XP_001160221.1
Mus musculus (house mouse)	Oxt	NP_035155.1
Rattus norvegicus (Norway rat)	Oxt	NP_037128.1
Xenopus tropicalis (tropical clawed frog)	LOC100485685	XP_002936405.1

Gene Ontology
Bibliography

Related articles in PubMed

Interrelationships between ovarian follicles grown in culture and possible mediators.
Sirotkin AV, Florkovi?ov? Koni?kov? I, Schaeffer HJ, Laurincik J, Harrath AH
Reproductive biology17(1)97-104(2017 Mar)

Adverse influence of coumestrol on secretory function of bovine luteal cells in the first trimester of pregnancy.
M?ynarczuk J, Wr?bel MH, Kotwica J
Environmental toxicology28(7)411-8(2013 Jul)

Effect of cortisol on neurophysin I/oxytocin and peptidyl glycine-alpha-amidating mono-oxygenase mRNA expression in bovine luteal and granulosa cells.
Ziolkowska A, Mlynarczuk J, Kotwica J
Polish journal of veterinary sciences16(2)231-9(2013)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The gene for the hypothalamic peptide hormone oxytocin is highly expressed in the bovine corpus luteum: biosynthesis, structure and sequence analysis.
Ivell R, Richter D
The EMBO journal3(10)2351-4(1984 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that both ovarian follicular dominance in cows and cooperation of ovarian follicles in pigs can be mediated by either down- or up-regulation of the insulin-like growth factor 1/oxytocin system.
Title: Interrelationships between ovarian follicles grown in culture and possible mediators.

Oxytocin (OT) mRNA and protein levels were lower in the corpus luteum of pregnancy than in the corpus luteum of the estrous cycle.
Title: Different expression of PGE synthase, PGF receptor, TNF, Fas and oxytocin in the bovine corpus luteum of the estrous cycle and pregnancy.

These data suggest that cortisol affects oxytocin synthesis and secretion in bovine ovarian cells, by acting on the expression of key genes, that may impair ovary
Title: Effect of cortisol on neurophysin I/oxytocin and peptidyl glycine-alpha-amidating mono-oxygenase mRNA expression in bovine luteal and granulosa cells.

Intraluteal OXT may locally modulate secretion of vasoactive substances, particularly EDN1 and PGF(2alpha) within the corpus luteum.
Title: Positive association, in local release, of luteal oxytocin with endothelin 1 and prostaglandin F2alpha during spontaneous luteolysis in the cow: a possible intermediatory role for luteolytic cascade within the corpus luteum.

loop status modulates dimerization via long-range effects on neurophysin conformation involving neighboring Phe22 as a key intermediary
Title: Contributions of the interdomain loop, amino terminus, and subunit interface to the ligand-facilitated dimerization of neurophysin: crystal structures and mutation studies of bovine neurophysin-I.

The following OXT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OXT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41377	NM_176855.1 Latest version!	Bos taurus oxytocin/neurophysin I prepropeptide (OXT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OXT ( NM_176855.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_789825.1 Bos taurus oxytocin/neurophysin I prepropeptide (OXT), mRNA.

Price & Availability↑

CloneID	OBa41377
Clone ID Related Accession (Same CDS sequence)	NM_176855.1
Accession Version	NM_176855.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	oxytocin-neurophysin 1 preproprotein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M25648.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M25648.1, DV839470.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145433, SAMN03145439 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGGTT CCAGCCTCGC CTGCTGCCTG CTCGGCCTCC TGGCGTTGAC CTCCGCCTGC 
TACATTCAGA ACTGCCCCCT GGGCGGCAAA CGCGCGGTGC TGGACCTCGA CGTGCGCACG 
TGTCTCCCCT GCGGCCCCGG GGGCAAAGGC CGCTGCTTCG GGCCCAGCAT CTGCTGCGGG 
GACGAGCTGG GCTGCTTCGT GGGCACGGCC GAGGCGCTGC GCTGCCAAGA GGAGAACTAC 
CTGCCGTCGC CCTGCCAGTC CGGCCAGAAG CCCTGCGGGA GCGGGGGCCG CTGCGCCGCC 
GCCGGCATCT GCTGCAGCCC GGACGGCTGC CACGAGGACC CCGCCTGCGA CCCTGAGGCC 
GCCTTCTCCC AGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_789825.1
CDS	34..411
Translation	MAGSSLACCLLGLLALTSACYIQNCPLGGKRAVLDLDVRTCLPCGPGGKGRCFGPSICCGDELGCFVGTAEALRCQEENYLPSPCQSGQKPCGSGGRCAAAGICCSPDGCHEDPACDPEAAFSQH

Target ORF information:

RefSeq Version	NM_176855.1
Organism	Bos taurus(cattle)
Definition	Bos taurus oxytocin/neurophysin I prepropeptide (OXT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176855.1

ORF Insert Sequence:

ATGGCAGGTT CCAGCCTCGC CTGCTGCCTG CTCGGCCTCC TGGCGTTGAC CTCCGCCTGC 
TACATTCAGA ACTGCCCCCT GGGCGGCAAA CGCGCGGTGC TGGACCTCGA CGTGCGCACG 
TGTCTCCCCT GCGGCCCCGG GGGCAAAGGC CGCTGCTTCG GGCCCAGCAT CTGCTGCGGG 
GACGAGCTGG GCTGCTTCGT GGGCACGGCC GAGGCGCTGC GCTGCCAAGA GGAGAACTAC 
CTGCCGTCGC CCTGCCAGTC CGGCCAGAAG CCCTGCGGGA GCGGGGGCCG CTGCGCCGCC 
GCCGGCATCT GCTGCAGCCC GGACGGCTGC CACGAGGACC CCGCCTGCGA CCCTGAGGCC 
GCCTTCTCCC AGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interrelationships between ovarian follicles grown in culture and possible mediators.
Reproductive biology17(1)97-104(2017 Mar)
Sirotkin AV,Florkovi?ov? Koni?kov? I,Schaeffer HJ,Laurincik J,Harrath AH

Adverse influence of coumestrol on secretory function of bovine luteal cells in the first trimester of pregnancy.
Environmental toxicology28(7)411-8(2013 Jul)
M?ynarczuk J,Wr?bel MH,Kotwica J

Effect of cortisol on neurophysin I/oxytocin and peptidyl glycine-alpha-amidating mono-oxygenase mRNA expression in bovine luteal and granulosa cells.
Polish journal of veterinary sciences16(2)231-9(2013)
Ziolkowska A,Mlynarczuk J,Kotwica J

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The gene for the hypothalamic peptide hormone oxytocin is highly expressed in the bovine corpus luteum: biosynthesis, structure and sequence analysis.
The EMBO journal3(10)2351-4(1984 Oct)
Ivell R,Richter D