NNT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NNT
Entrez Gene ID	280878
Full Name	nicotinamide nucleotide transhydrogenase
General protein information	Preferred Names nicotinamide nucleotide transhydrogenase Names NAD(P) transhydrogenase, mitochondrial pyridine nucleotide transhydrogenase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221529.3	XP_005221586.1	NAD(P) transhydrogenase, mitochondrial isoform X1
XM_005221528.3	XP_005221585.1	NAD(P) transhydrogenase, mitochondrial isoform X1
XM_024981195.1	XP_024836963.1	NAD(P) transhydrogenase, mitochondrial isoform X1
XM_005221530.3	XP_005221587.1	NAD(P) transhydrogenase, mitochondrial isoform X1
NM_173943.3	NP_776368.1	NAD(P) transhydrogenase, mitochondrial precursor
XM_005221529.4	XP_005221586.1	NAD(P) transhydrogenase, mitochondrial isoform X1
XM_005221530.4	XP_005221587.1	NAD(P) transhydrogenase, mitochondrial isoform X1
XM_005221528.4	XP_005221585.1	NAD(P) transhydrogenase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003167	XP_312859.4
Xenopus tropicalis (tropical clawed frog)	nnt	NP_001121474.1
Homo sapiens (human)	NNT	NP_036475.3
Canis lupus familiaris (dog)	NNT	XP_536481.3
Neurospora crassa	NCU01140	XP_961047.2
Pan troglodytes (chimpanzee)	NNT	XP_517776.2
Danio rerio (zebrafish)	nnt	NP_999921.1
Caenorhabditis elegans (roundworm)	nnt-1	NP_509028.1
Magnaporthe oryzae (rice blast fungus)	MGG_07539	XP_003711452.1
Bos taurus (cattle)	NNT	NP_776368.1
	NNT	XP_002804397.1
Rattus norvegicus (Norway rat)	Nnt	NP_001013175.1
Gallus gallus (chicken)	NNT	XP_003642984.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Crystal structure of transhydrogenase domain III at 1.2 A resolution.
Prasad GS, Sridhar V, Yamaguchi M, Hatefi Y, Stout CD
Nature structural biology6(12)1126-31(1999 Dec)

Prediction and site-specific mutagenesis of residues in transmembrane alpha-helices of proton-pumping nicotinamide nucleotide transhydrogenases from Escherichia coli and bovine heart mitochondria.
Holmberg E, Olausson T, Hultman T, Rydstr?m J, Ahmad S, Glavas NA, Bragg PD
Biochemistry33(24)7691-700(1994 Jun)

Characterization of the substrate-binding sites of the mitochondrial nicotinamide nucleotide transhydrogenase.
Wakabayashi S, Hatefi Y
Biochemistry international15(5)915-24(1987 Nov)

Amino acid sequence of the NAD (H)--binding region of the mitochondrial nicotinamide nucleotide transhydrogenase modified by N,N'-dicyclohexylcarbodiimide.
Wakabayashi S, Hatefi Y
Biochemistry international15(3)667-75(1987 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NNT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NNT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00253	NM_173943.3 Latest version!	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30625	XM_024981195.1 Latest version!	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30625	XM_005221528.4 Latest version!	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30625	XM_005221529.4 Latest version!	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30625	XM_005221530.4 Latest version!	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa30625	XM_005221529.3	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30625	XM_005221530.3	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30625	XM_005221528.3	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NNT ( NM_173943.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776368.1 Bos taurus nicotinamide nucleotide transhydrogenase (NNT), mRNA.

Price & Availability↑

CloneID	OBa00253
Clone ID Related Accession (Same CDS sequence)	NM_173943.3
Accession Version	NM_173943.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	NAD(P) transhydrogenase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03534.1. On Feb 21, 2013 this sequence version replaced NM_173943.2. ##Evidence-Data-START## Transcript exon combination :: J03534.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTACG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776368.1
CDS	55..3315
Translation	MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCSAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGVHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAGSVGGIIPFMMDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	NM_173943.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173943.3

ORF Insert Sequence:

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTACG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_005221529.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221586.1 Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30625
Clone ID Related Accession (Same CDS sequence)	XM_005221529.3 , XM_005221528.3 , XM_024981195.1 , XM_005221530.3 , XM_005221529.4 , XM_005221530.4 , XM_005221528.4
Accession Version	XM_005221529.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NAD(P) transhydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104513.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221529.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221586.1
CDS	215..3475
Translation	MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCSAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGVHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAGSVGGIIPFMMDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_005221529.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221529.3

ORF Insert Sequence:

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_005221528.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221585.1 Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30625
Clone ID Related Accession (Same CDS sequence)	XM_005221529.3 , XM_005221528.3 , XM_024981195.1 , XM_005221530.3 , XM_005221529.4 , XM_005221530.4 , XM_005221528.4
Accession Version	XM_005221528.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NAD(P) transhydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104513.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221585.1
CDS	174..3434
Translation	MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCSAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGVHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAGSVGGIIPFMMDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_005221528.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221528.3

ORF Insert Sequence:

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_024981195.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836963.1 Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa30625
Clone ID Related Accession (Same CDS sequence)	XM_005221529.3 , XM_005221528.3 , XM_024981195.1 , XM_005221530.3 , XM_005221529.4 , XM_005221530.4 , XM_005221528.4
Accession Version	XM_024981195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD(P) transhydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836963.1
CDS	308..3568
Translation	MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCSAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGVHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAGSVGGIIPFMMDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_024981195.1
Organism	Bos taurus(cattle)
Definition	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981195.1

ORF Insert Sequence:

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_005221530.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221587.1 Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X3, mRNA.

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CloneID	OBa30625
Clone ID Related Accession (Same CDS sequence)	XM_005221529.3 , XM_005221528.3 , XM_024981195.1 , XM_005221530.3 , XM_005221529.4 , XM_005221530.4 , XM_005221528.4
Accession Version	XM_005221530.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NAD(P) transhydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104513.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221587.1
CDS	168..3428
Translation	MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCSAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGVHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAGSVGGIIPFMMDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_005221530.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221530.3

ORF Insert Sequence:

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_005221529.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221586.1 Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30625
Clone ID Related Accession (Same CDS sequence)	XM_005221529.3 , XM_005221528.3 , XM_024981195.1 , XM_005221530.3 , XM_005221529.4 , XM_005221530.4 , XM_005221528.4
Accession Version	XM_005221529.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD(P) transhydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221529.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221586.1
CDS	385..3645
Translation	MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCSAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGVHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAGSVGGIIPFMMDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_005221529.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221529.4

ORF Insert Sequence:

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_005221530.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221587.1 Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30625
Clone ID Related Accession (Same CDS sequence)	XM_005221529.3 , XM_005221528.3 , XM_024981195.1 , XM_005221530.3 , XM_005221529.4 , XM_005221530.4 , XM_005221528.4
Accession Version	XM_005221530.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD(P) transhydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221530.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221587.1
CDS	254..3514
Translation	MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCSAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGVHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAGSVGGIIPFMMDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_005221530.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221530.4

ORF Insert Sequence:

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NNT ( XM_005221528.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221585.1 Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.

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CloneID	OBa30625
Clone ID Related Accession (Same CDS sequence)	XM_005221529.3 , XM_005221528.3 , XM_024981195.1 , XM_005221530.3 , XM_005221529.4 , XM_005221530.4 , XM_005221528.4
Accession Version	XM_005221528.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NAD(P) transhydrogenase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221528.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221585.1
CDS	178..3438
Translation	MANLLKTVVTGCSCPFLSNLGSCKVLPGKKNFLRTFHTHRILWCSAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQALVKQGFNVVVESGAGEASKFSDDHYRAAGAQIQGAKEVLASDLVVKVRAPMLNPTLGVHEADLLKTSGTLISFIYPAQNPDLLNKLSKRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPILFNKEMIESMKEGSVVVDLAAEAGGNFETTKPGELYVHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFEVKDDFDFGTMGHVIRGTVVMKDGQVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMTSASVYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLVLMGGHLYPSTTSQGLAALATFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLASLYSGYNIEQIMYLGSGLCCVGALAGLSTQGTARLGNALGMIGVAGGLAATLGGLKPCPELLAQMSGAMALGGTIGLTIAKRIQISDLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLVAYGKLQGILKSAPLLLPGRHLLNAGLLAGSVGGIIPFMMDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLSEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK

Target ORF information:

RefSeq Version	XM_005221528.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nicotinamide nucleotide transhydrogenase (NNT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221528.4

ORF Insert Sequence:

ATGGCGAACC TACTGAAAAC AGTGGTGACT GGTTGTTCAT GCCCTTTCCT CAGCAATTTG 
GGGTCCTGTA AGGTTCTACC AGGGAAGAAG AATTTTTTAC GAACATTTCA TACTCATCGG 
ATACTGTGGT GTAGTGCCCC TGTAAAACCA GGAATTCCAT ACAAGCAACT AACTGTTGGG 
GTCCCCAAAG AGATTTTCCA AAATGAGAAG CGAGTGGCAT TGTCTCCTGC TGGCGTTCAG 
GCCTTGGTCA AGCAGGGGTT TAATGTTGTT GTGGAATCTG GCGCAGGTGA AGCTTCCAAG 
TTCTCAGATG ATCACTACAG AGCAGCAGGT GCCCAGATCC AAGGGGCGAA GGAAGTGCTG 
GCTTCTGATT TGGTGGTCAA AGTGCGAGCC CCTATGCTTA ACCCAACATT AGGTGTTCAT 
GAAGCTGATC TTTTAAAGAC ATCGGGTACA CTGATCAGTT TTATTTACCC AGCCCAAAAT 
CCAGACTTGC TAAATAAACT GTCCAAAAGG AAGACGACAG TTCTGGCAAT GGACCAGGTT 
CCAAGAGTCA CAATTGCTCA GGGATATGAT GCGCTAAGCT CCATGGCCAA CATTGCCGGT 
TACAAGGCTG TTGTACTAGC AGCAAATCAT TTTGGACGGT TTTTTACTGG TCAGATCACA 
GCTGCTGGAA AAGTTCCTCC AGCTAAGATT CTGATAGTTG GTGGTGGCGT TGCTGGGCTG 
GCTTCTGCAG GTGCAGCCAA GTCCATGGGT GCCATTGTTA GGGGATTTGA TACAAGGGCC 
GCAGCTTTGG AGCAGTTTAA GTCTTTGGGT GCTGAGCCCT TGGAGGTGGA TTTGAAGGAA 
TCTGGTGAGG GACAAGGAGG ATACGCAAAG GAGATGTCCA AAGAGTTCAT TGAGGCCGAA 
ATGAAGCTCT TTGCTCAACA GTGTAAGGAG GTGGACATCC TTATCAGCAC AGCTCTTATT 
CCAGGTAAAA AAGCTCCCAT TTTGTTTAAT AAAGAAATGA TTGAGTCCAT GAAGGAAGGT 
TCAGTTGTTG TGGATTTAGC TGCTGAGGCT GGCGGAAATT TTGAAACTAC CAAGCCAGGA 
GAACTTTATG TTCATAAGGG AATTACTCAC ATAGGCTACA CAGACCTTCC CAGCCGAATG 
GCCACTCAGG CCAGCACCCT GTATTCCAAC AACATCACCA AGCTTCTGAA GGCCATCAGC 
CCAGACAAAG ATAATTTTTA CTTTGAAGTG AAAGATGACT TTGACTTTGG TACAATGGGT 
CACGTCATTA GAGGAACCGT AGTGATGAAG GATGGCCAAG TGATTTTCCC TGCTCCCACA 
CCGAAAAATA TTCCTCAAGG TGCCCCAGTA AAACAGAAGA CAGTGGCAGA GCTGGAGGCT 
GAAAAAGCAG CTACTATCAC GCCCTTCAGG AAGACGATGA CATCGGCTTC TGTCTATACA 
GCAGGTCTCA CTGGGATACT AGGTTTGGGC ATCGCGGCTC CCAATTTAGC CTTTTCCCAG 
ATGGTGACCA CCTTCGGCTT GGCTGGCATT GTGGGCTACC ATACTGTCTG GGGAGTGACT 
CCTGCTCTCC ACTCACCGCT AATGTCTGTG ACTAATGCAA TCTCAGGGTT GACTGCAGTC 
GGTGGGTTGG TGCTGATGGG AGGACACTTG TATCCTTCCA CAACTTCTCA GGGCCTTGCT 
GCTCTTGCCA CGTTCATATC CTCAGTCAAC ATTGCAGGTG GCTTTCTGGT GACTCAGAGA 
ATGCTGGACA TGTTCAAGCG TCCCACTGAC CCTCCGGAGT ATAATTACCT GTATCTGCTT 
CCTGCTGGTA CCTTTGTTGG GGGATATTTA GCCTCCCTCT ACAGTGGTTA TAACATTGAA 
CAGATTATGT ACCTGGGCTC CGGTCTTTGC TGTGTTGGTG CCCTGGCCGG GCTTTCCACC 
CAGGGGACTG CACGTCTTGG CAATGCCCTG GGCATGATTG GGGTGGCTGG AGGCTTGGCA 
GCCACCCTCG GAGGCCTAAA ACCATGTCCA GAATTGTTAG CTCAGATGTC TGGAGCAATG 
GCTTTGGGCG GTACCATTGG ATTGACAATT GCGAAACGCA TCCAGATTTC GGATTTACCT 
CAGTTAGTTG CTGCTTTCCA CAGTTTAGTG GGATTGGCAG CTGTGCTTAC GTGCATAGCT 
GAGTACATCA TAGAATATCC ACATTTCGCT ACGGATGCAG CAGCCAACCT TACCAAGATC 
GTGGCCTACC TCGGTACTTA CATTGGTGGG GTGACCTTCA GTGGGTCTCT GGTTGCCTAT 
GGAAAATTGC AGGGAATCCT GAAGTCCGCC CCTCTCCTGC TCCCTGGGAG GCACCTCCTC 
AATGCAGGCC TGCTGGCTGG TAGCGTGGGT GGGATCATCC CCTTCATGAT GGACCCCAGC 
TTCACCACTG GCATCACCTG TCTGGGCTCT GTGTCAGCCC TCTCTGCTGT CATGGGTGTG 
ACTCTGACGG CTGCCATTGG GGGTGCCGAC ATGCCGGTGG TCATCACTGT GCTGAACAGC 
TACTCAGGCT GGGCCCTGTG TGCAGAGGGT TTCCTGCTCA ACAACAATCT GCTGACCATT 
GTGGGTGCGC TTATTGGCTC CTCGGGCGCA ATCCTGTCCT ACATCATGTG TGTGGCAATG 
AATCGCTCCC TGGCTAATGT GATTCTTGGA GGCTATGGTA CCACTTCAAC AGCTGGTGGA 
AAACCCATGG AAATTTCTGG CACGCATACA GAAATCAACC TTGACAATGC AATTGACATG 
ATTCGAGAAG CTAATAGCAT TATTATTACT CCAGGCTACG GGCTCTGTGC AGCCAAAGCT 
CAGTACCCCA TTGCTGATTT GGTGAAGATG CTCTCTGAAC AAGGCAAAAA AGTCAGGTTT 
GGAATTCACC CAGTTGCAGG CCGGATGCCT GGTCAGCTTA ATGTGCTGCT GGCTGAGGCT 
GGGGTGCCAT ATGATATTGT GTTGGAAATG GACGAGATCA ATCATGATTT CCCAGACACT 
GATTTGGTCC TTGTAATTGG AGCTAATGAC ACTGTTAATT CAGCAGCTCA AGAAGACCCC 
AACTCCATCA TTGCAGGCAT GCCAGTCCTT GAAGTCTGGA AATCAAAGCA GGTCATTGTT 
ATGAAGAGGT CCCTAGGTGT TGGCTATGCT GCAGTGGACA ATCCAATCTT CTACAAACCG 
AACACAGCCA TGCTTCTAGG CGATGCCAAG AAGACGTGTG ATGCTCTGCA GGCGAAAGTT 
AGAGAATCCT ACCAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Crystal structure of transhydrogenase domain III at 1.2 A resolution.
Nature structural biology6(12)1126-31(1999 Dec)
Prasad GS,Sridhar V,Yamaguchi M,Hatefi Y,Stout CD

Prediction and site-specific mutagenesis of residues in transmembrane alpha-helices of proton-pumping nicotinamide nucleotide transhydrogenases from Escherichia coli and bovine heart mitochondria.
Biochemistry33(24)7691-700(1994 Jun)
Holmberg E,Olausson T,Hultman T,Rydstr?m J,Ahmad S,Glavas NA,Bragg PD

Characterization of the substrate-binding sites of the mitochondrial nicotinamide nucleotide transhydrogenase.
Biochemistry international15(5)915-24(1987 Nov)
Wakabayashi S,Hatefi Y

Amino acid sequence of the NAD (H)--binding region of the mitochondrial nicotinamide nucleotide transhydrogenase modified by N,N'-dicyclohexylcarbodiimide.
Biochemistry international15(3)667-75(1987 Sep)
Wakabayashi S,Hatefi Y

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NNT cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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