GAA cDNA ORF clone, Bos taurus(cattle)

The following GAA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa30331 NM_173913.2
Latest version!
Bos taurus glucosidase alpha, acid (GAA), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30330 XM_005221135.4
Latest version!
Bos taurus glucosidase alpha, acid (GAA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30330 XM_010816506.3
Latest version!
Bos taurus glucosidase alpha, acid (GAA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30331 XM_005221136.4
Latest version!
Bos taurus glucosidase alpha, acid (GAA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30331 XM_024979534.1
Latest version!
Bos taurus glucosidase alpha, acid (GAA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OBa30330 XM_005221135.3 Bos taurus glucosidase, alpha; acid (GAA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30331 XM_005221136.3 Bos taurus glucosidase, alpha; acid (GAA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa30330 XM_010816506.2 Bos taurus glucosidase, alpha; acid (GAA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa30331
    Clone ID Related Accession (Same CDS sequence) XM_005221136.4 , NM_173913.2 , XM_005221136.3 , XM_024979534.1
    Accession Version NM_173913.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Bos taurus(cattle)
    Product lysosomal alpha-glucosidase precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF171665.1. On Jun 3, 2007 this sequence version replaced NM_173913.1. ##Evidence-Data-START## Transcript exon combination :: AF171665.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG
    GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776338.1
    CDS1..2814
    Translation

    Target ORF information:

    RefSeq Version NM_173913.2
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase alpha, acid (GAA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_173913.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG
    GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30330
    Clone ID Related Accession (Same CDS sequence) XM_005221135.3 , XM_005221135.4 , XM_010816506.2 , XM_010816506.3
    Accession Version XM_005221135.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA
    GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT
    GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC
    TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG
    GGTCAGAAGT GCTTACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221192.1
    CDS124..3084
    Translation

    Target ORF information:

    RefSeq Version XM_005221135.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase, alpha; acid (GAA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221135.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA
    GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT
    GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC
    TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG
    GGTCAGAAGT GCTTACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30330
    Clone ID Related Accession (Same CDS sequence) XM_005221135.3 , XM_005221135.4 , XM_010816506.2 , XM_010816506.3
    Accession Version XM_005221135.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221135.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA
    GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT
    GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC
    TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG
    GGTCAGAAGT GCTTACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221192.1
    CDS127..3087
    Translation

    Target ORF information:

    RefSeq Version XM_005221135.4
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase alpha, acid (GAA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221135.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA
    GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT
    GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC
    TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG
    GGTCAGAAGT GCTTACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30330
    Clone ID Related Accession (Same CDS sequence) XM_005221135.3 , XM_005221135.4 , XM_010816506.2 , XM_010816506.3
    Accession Version XM_010816506.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA
    GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT
    GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC
    TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG
    GGTCAGAAGT GCTTACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814808.1
    CDS230..3190
    Translation

    Target ORF information:

    RefSeq Version XM_010816506.2
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase, alpha; acid (GAA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816506.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA
    GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT
    GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC
    TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG
    GGTCAGAAGT GCTTACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30330
    Clone ID Related Accession (Same CDS sequence) XM_005221135.3 , XM_005221135.4 , XM_010816506.2 , XM_010816506.3
    Accession Version XM_010816506.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysosomal alpha-glucosidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816506.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA
    GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT
    GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC
    TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG
    GGTCAGAAGT GCTTACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814808.1
    CDS246..3206
    Translation

    Target ORF information:

    RefSeq Version XM_010816506.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase alpha, acid (GAA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816506.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA
    GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT
    GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC
    TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG
    GGTCAGAAGT GCTTACCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30331
    Clone ID Related Accession (Same CDS sequence) XM_005221136.4 , NM_173913.2 , XM_005221136.3 , XM_024979534.1
    Accession Version XM_005221136.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysosomal alpha-glucosidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221136.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG
    GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221193.1
    CDS127..2940
    Translation

    Target ORF information:

    RefSeq Version XM_005221136.4
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase alpha, acid (GAA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221136.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG
    GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30331
    Clone ID Related Accession (Same CDS sequence) XM_005221136.4 , NM_173913.2 , XM_005221136.3 , XM_024979534.1
    Accession Version XM_005221136.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysosomal alpha-glucosidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG
    GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221193.1
    CDS124..2937
    Translation

    Target ORF information:

    RefSeq Version XM_005221136.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase, alpha; acid (GAA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221136.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG
    GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30331
    Clone ID Related Accession (Same CDS sequence) XM_005221136.4 , NM_173913.2 , XM_005221136.3 , XM_024979534.1
    Accession Version XM_024979534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2814bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysosomal alpha-glucosidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG
    GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024835302.1
    CDS253..3066
    Translation

    Target ORF information:

    RefSeq Version XM_024979534.1
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase alpha, acid (GAA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024979534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
    GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC
    TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT
    GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC
    GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT
    GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC
    CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC
    CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG
    GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG
    CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC
    TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC
    ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA
    TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG
    ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA
    TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG
    AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA
    GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG
    TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG
    TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG
    GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC
    TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG
    GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG
    ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG
    CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC
    GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT
    GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC
    CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG
    GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC
    CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG
    CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG
    ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT
    TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC
    TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC
    CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA
    GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC
    CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG
    GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC
    CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC
    GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG
    TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC
    GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG
    TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG
    GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG
    CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG
    GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG
    GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG
    GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.