GAA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAA
Entrez Gene ID	280798
Full Name	glucosidase alpha, acid
General protein information	Preferred Names glucosidase alpha, acid Names lysosomal alpha-glucosidase acid maltase glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221135.3	XP_005221192.1	lysosomal alpha-glucosidase isoform X1
XM_005221135.4	XP_005221192.1	lysosomal alpha-glucosidase isoform X1
XM_005221136.4	XP_005221193.1	lysosomal alpha-glucosidase isoform X2
NM_173913.2	NP_776338.1	lysosomal alpha-glucosidase precursor
XM_010816506.2	XP_010814808.1	lysosomal alpha-glucosidase isoform X1
XM_005221136.3	XP_005221193.1	lysosomal alpha-glucosidase isoform X2
XM_024979534.1	XP_024835302.1	lysosomal alpha-glucosidase isoform X2
XM_010816506.3	XP_010814808.1	lysosomal alpha-glucosidase isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC422082	XP_420085.4
Schizosaccharomyces pombe (fission yeast)	SPAC30D11.01c	NP_593216.2
Canis lupus familiaris (dog)	GAA	XP_005624079.1
Mus musculus (house mouse)	Gaa	NP_032090.3
Pan troglodytes (chimpanzee)	GAA	XP_001160653.1
Rattus norvegicus (Norway rat)	Gaa	NP_954549.1
Danio rerio (zebrafish)	gaa	XP_001921957.2
Schizosaccharomyces pombe (fission yeast)	agl1	NP_593996.1
Oryza sativa (rice)	Os01g0130400	NP_001041922.1
Bos taurus (cattle)	GAA	NP_776338.1
Schizosaccharomyces pombe (fission yeast)	SPAC1039.11c	XP_001713119.1
Neurospora crassa	NCU09281	XP_958500.1
Xenopus tropicalis (tropical clawed frog)	gaa	XP_002932561.2
Homo sapiens (human)	GAA	NP_001073272.1
	GAA	XP_001109980.1
Magnaporthe oryzae (rice blast fungus)	MGG_10662	XP_003710096.1
Arabidopsis thaliana (thale cress)	XYL1	NP_177023.1
Arabidopsis thaliana (thale cress)	AT3G45940	NP_190180.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The bovine alpha-glucosidase gene: coding region, genomic structure, and mutations that cause bovine generalized glycogenosis.
Dennis JA, Moran C, Healy PJ
Mammalian genome : official journal of the International Mammalian Genome Society11(3)206-12(2000 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The lethal mutation 1057DeltaTA of GAA is present in the Droughtmaster breed, with pathology identical to that reported in pure Brahman animals.
Title: E7 (1057?TA) mutation of the acidic ?-glucosidase gene causes Pompe's disease in Droughtmaster cattle.

The following GAA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa30331	NM_173913.2 Latest version!	Bos taurus glucosidase alpha, acid (GAA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa30330	XM_005221135.4 Latest version!	Bos taurus glucosidase alpha, acid (GAA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa30330	XM_010816506.3 Latest version!	Bos taurus glucosidase alpha, acid (GAA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa30331	XM_005221136.4 Latest version!	Bos taurus glucosidase alpha, acid (GAA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa30331	XM_024979534.1 Latest version!	Bos taurus glucosidase alpha, acid (GAA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OBa30330	XM_005221135.3	Bos taurus glucosidase, alpha; acid (GAA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa30331	XM_005221136.3	Bos taurus glucosidase, alpha; acid (GAA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa30330	XM_010816506.2	Bos taurus glucosidase, alpha; acid (GAA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAA ( NM_173913.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776338.1 Bos taurus glucosidase alpha, acid (GAA), mRNA.

Price & Availability↑

CloneID	OBa30331
Clone ID Related Accession (Same CDS sequence)	XM_005221136.4 , NM_173913.2 , XM_005221136.3 , XM_024979534.1
Accession Version	NM_173913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	lysosomal alpha-glucosidase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF171665.1. On Jun 3, 2007 this sequence version replaced NM_173913.1. ##Evidence-Data-START## Transcript exon combination :: AF171665.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG 
GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776338.1
CDS	1..2814
Translation	MMRWPPCSRPLLGVCTLLSLALLGHILLHDLEVVPRELRGFSQDEIHQACQPGASSPECRGSPRAAPTQCDLPPNSRFDCAPDKGITPQQCEARGCCYMPAEWPPDAQMGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLETPRVYSQAPFTLYSVEFSEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLMLSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQGGRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITNETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNSLENPPYLPGVVGGTLRAATICASSHQFLSTHYDLHNLYGLTEALASHRALVKARGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHSKGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLAKNNTFVNKLVHVSSEGASLQLRNVTVLGVATAPQQVLCNSVPVSNFTFSPDTETLAIPVSLTMGEQFVISWS

Target ORF information:

RefSeq Version	NM_173913.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glucosidase alpha, acid (GAA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173913.2

ORF Insert Sequence:

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG 
GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_005221135.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221192.1 Bos taurus glucosidase, alpha; acid (GAA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30330
Clone ID Related Accession (Same CDS sequence)	XM_005221135.3 , XM_005221135.4 , XM_010816506.2 , XM_010816506.3
Accession Version	XM_005221135.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysosomal alpha-glucosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA 
GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT 
GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC 
TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG 
GGTCAGAAGT GCTTACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221192.1
CDS	124..3084
Translation	MMRWPPCSRPLLGVCTLLSLALLGHILLHDLEVVPRELRGFSQDEIHQACQPGASSPECRGSPRAAPTQCDLPPNSRFDCAPDKGITPQQCEARGCCYMPAEWPPDAQMGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLETPRVYSQAPFTLYSVEFSEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLMLSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQGGRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITNETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNSLENPPYLPGVVGGTLRAATICASSHQFLSTHYDLHNLYGLTEALASHRALVKARGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHSKGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLAKNNTFVNKLVHVSSEGASLQLRNVTVLGVATAPQQVLCNSVPVSNFTFSPDTEAREPTIGPRGPIPEDSGHPCLADHGRAVCHQLVLTRGPAVCCSFTDLPVAQHLHPVGGVGALGQKCLP

Target ORF information:

RefSeq Version	XM_005221135.3
Organism	Bos taurus(cattle)
Definition	Bos taurus glucosidase, alpha; acid (GAA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221135.3

ORF Insert Sequence:

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA 
GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT 
GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC 
TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG 
GGTCAGAAGT GCTTACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_005221135.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221192.1 Bos taurus glucosidase alpha, acid (GAA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30330
Clone ID Related Accession (Same CDS sequence)	XM_005221135.3 , XM_005221135.4 , XM_010816506.2 , XM_010816506.3
Accession Version	XM_005221135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysosomal alpha-glucosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221135.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA 
GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT 
GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC 
TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG 
GGTCAGAAGT GCTTACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221192.1
CDS	127..3087
Translation	MMRWPPCSRPLLGVCTLLSLALLGHILLHDLEVVPRELRGFSQDEIHQACQPGASSPECRGSPRAAPTQCDLPPNSRFDCAPDKGITPQQCEARGCCYMPAEWPPDAQMGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLETPRVYSQAPFTLYSVEFSEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLMLSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQGGRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITNETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNSLENPPYLPGVVGGTLRAATICASSHQFLSTHYDLHNLYGLTEALASHRALVKARGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHSKGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLAKNNTFVNKLVHVSSEGASLQLRNVTVLGVATAPQQVLCNSVPVSNFTFSPDTEAREPTIGPRGPIPEDSGHPCLADHGRAVCHQLVLTRGPAVCCSFTDLPVAQHLHPVGGVGALGQKCLP

Target ORF information:

RefSeq Version	XM_005221135.4
Organism	Bos taurus(cattle)
Definition	Bos taurus glucosidase alpha, acid (GAA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221135.4

ORF Insert Sequence:

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA 
GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT 
GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC 
TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG 
GGTCAGAAGT GCTTACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_010816506.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814808.1 Bos taurus glucosidase, alpha; acid (GAA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30330
Clone ID Related Accession (Same CDS sequence)	XM_005221135.3 , XM_005221135.4 , XM_010816506.2 , XM_010816506.3
Accession Version	XM_010816506.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysosomal alpha-glucosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA 
GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT 
GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC 
TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG 
GGTCAGAAGT GCTTACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814808.1
CDS	230..3190
Translation	MMRWPPCSRPLLGVCTLLSLALLGHILLHDLEVVPRELRGFSQDEIHQACQPGASSPECRGSPRAAPTQCDLPPNSRFDCAPDKGITPQQCEARGCCYMPAEWPPDAQMGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLETPRVYSQAPFTLYSVEFSEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLMLSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQGGRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITNETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNSLENPPYLPGVVGGTLRAATICASSHQFLSTHYDLHNLYGLTEALASHRALVKARGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHSKGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLAKNNTFVNKLVHVSSEGASLQLRNVTVLGVATAPQQVLCNSVPVSNFTFSPDTEAREPTIGPRGPIPEDSGHPCLADHGRAVCHQLVLTRGPAVCCSFTDLPVAQHLHPVGGVGALGQKCLP

Target ORF information:

RefSeq Version	XM_010816506.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glucosidase, alpha; acid (GAA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816506.2

ORF Insert Sequence:

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA 
GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT 
GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC 
TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG 
GGTCAGAAGT GCTTACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_010816506.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814808.1 Bos taurus glucosidase alpha, acid (GAA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30330
Clone ID Related Accession (Same CDS sequence)	XM_005221135.3 , XM_005221135.4 , XM_010816506.2 , XM_010816506.3
Accession Version	XM_010816506.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysosomal alpha-glucosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816506.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA 
GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT 
GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC 
TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG 
GGTCAGAAGT GCTTACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814808.1
CDS	246..3206
Translation	MMRWPPCSRPLLGVCTLLSLALLGHILLHDLEVVPRELRGFSQDEIHQACQPGASSPECRGSPRAAPTQCDLPPNSRFDCAPDKGITPQQCEARGCCYMPAEWPPDAQMGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLETPRVYSQAPFTLYSVEFSEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLMLSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQGGRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITNETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNSLENPPYLPGVVGGTLRAATICASSHQFLSTHYDLHNLYGLTEALASHRALVKARGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHSKGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLAKNNTFVNKLVHVSSEGASLQLRNVTVLGVATAPQQVLCNSVPVSNFTFSPDTEAREPTIGPRGPIPEDSGHPCLADHGRAVCHQLVLTRGPAVCCSFTDLPVAQHLHPVGGVGALGQKCLP

Target ORF information:

RefSeq Version	XM_010816506.3
Organism	Bos taurus(cattle)
Definition	Bos taurus glucosidase alpha, acid (GAA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816506.3

ORF Insert Sequence:

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGGCAAGA 
GAGCCCACAA TTGGGCCCAG AGGGCCTATC CCTGAGGACT CTGGCCATCC CTGTCTCGCT 
GACCATGGGA GAGCAGTTTG TCATCAGCTG GTCTTAACCC GGGGCCCAGC GGTGTGCTGC 
TCCTTCACAG ACCTCCCAGT GGCCCAGCAC TTGCACCCTG TCGGCGGTGT AGGGGCCTTG 
GGTCAGAAGT GCTTACCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_005221136.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221193.1 Bos taurus glucosidase alpha, acid (GAA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30331
Clone ID Related Accession (Same CDS sequence)	XM_005221136.4 , NM_173913.2 , XM_005221136.3 , XM_024979534.1
Accession Version	XM_005221136.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysosomal alpha-glucosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221136.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG 
GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221193.1
CDS	127..2940
Translation	MMRWPPCSRPLLGVCTLLSLALLGHILLHDLEVVPRELRGFSQDEIHQACQPGASSPECRGSPRAAPTQCDLPPNSRFDCAPDKGITPQQCEARGCCYMPAEWPPDAQMGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLETPRVYSQAPFTLYSVEFSEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLMLSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQGGRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITNETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNSLENPPYLPGVVGGTLRAATICASSHQFLSTHYDLHNLYGLTEALASHRALVKARGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHSKGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLAKNNTFVNKLVHVSSEGASLQLRNVTVLGVATAPQQVLCNSVPVSNFTFSPDTETLAIPVSLTMGEQFVISWS

Target ORF information:

RefSeq Version	XM_005221136.4
Organism	Bos taurus(cattle)
Definition	Bos taurus glucosidase alpha, acid (GAA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221136.4

ORF Insert Sequence:

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG 
GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_005221136.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221193.1 Bos taurus glucosidase, alpha; acid (GAA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30331
Clone ID Related Accession (Same CDS sequence)	XM_005221136.4 , NM_173913.2 , XM_005221136.3 , XM_024979534.1
Accession Version	XM_005221136.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	lysosomal alpha-glucosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG 
GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221193.1
CDS	124..2937
Translation	MMRWPPCSRPLLGVCTLLSLALLGHILLHDLEVVPRELRGFSQDEIHQACQPGASSPECRGSPRAAPTQCDLPPNSRFDCAPDKGITPQQCEARGCCYMPAEWPPDAQMGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLETPRVYSQAPFTLYSVEFSEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLMLSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQGGRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITNETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNSLENPPYLPGVVGGTLRAATICASSHQFLSTHYDLHNLYGLTEALASHRALVKARGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHSKGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLAKNNTFVNKLVHVSSEGASLQLRNVTVLGVATAPQQVLCNSVPVSNFTFSPDTETLAIPVSLTMGEQFVISWS

Target ORF information:

RefSeq Version	XM_005221136.3
Organism	Bos taurus(cattle)
Definition	Bos taurus glucosidase, alpha; acid (GAA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221136.3

ORF Insert Sequence:

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG 
GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_024979534.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835302.1 Bos taurus glucosidase alpha, acid (GAA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa30331
Clone ID Related Accession (Same CDS sequence)	XM_005221136.4 , NM_173913.2 , XM_005221136.3 , XM_024979534.1
Accession Version	XM_024979534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	lysosomal alpha-glucosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG 
GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835302.1
CDS	253..3066
Translation	MMRWPPCSRPLLGVCTLLSLALLGHILLHDLEVVPRELRGFSQDEIHQACQPGASSPECRGSPRAAPTQCDLPPNSRFDCAPDKGITPQQCEARGCCYMPAEWPPDAQMGQPWCFFPPSYPSYRLENLTTTETGYTATLTRAVPTFFPKDIMTLRLDMLMETESRLHFTIKDPANRRYEVPLETPRVYSQAPFTLYSVEFSEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQHITGLAEHLGSLMLSTNWTKITLWNRDIAPEPNVNLYGSHPFYLVLEDGGLAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSTSAITRQVVENMTRAYFPLDVQWNDLDYMDARRDFTFNKDHFGDFPAMVQELHQGGRRYIMIVDPAISSSGPAGTYRPYDEGLRRGVFITNETGQPLIGQVWPGLTAFPDFTNPETLDWWQDMVTEFHAQVPFDGMWIDMNEPSNFVRGSVDGCPDNSLENPPYLPGVVGGTLRAATICASSHQFLSTHYDLHNLYGLTEALASHRALVKARGMRPFVISRSTFAGHGRYSGHWTGDVWSNWEQLSYSVPEILLFNLLGVPLVGADICGFLGNTSEELCVRWTQLGAFYPFMRNHNALNSQPQEPYRFSETAQQAMRKAFTLRYVLLPYLYTLFHRAHVRGETVARPLFLEFPEDPSTWTVDRQLLWGEALLITPVLEAEKVEVTGYFPQGTWYDLQTVPMEAFGSLPPPAPLTSVIHSKGQWVTLSAPLDTINVHLRAGHIIPMQGPALTTTESRKQHMALAVALTASGEAQGELFWDDGESLGVLDGGDYTQLIFLAKNNTFVNKLVHVSSEGASLQLRNVTVLGVATAPQQVLCNSVPVSNFTFSPDTETLAIPVSLTMGEQFVISWS

Target ORF information:

RefSeq Version	XM_024979534.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glucosidase alpha, acid (GAA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979534.1

ORF Insert Sequence:

ATGATGAGGT GGCCACCATG CTCCCGCCCC CTGCTGGGGG TCTGCACCCT CCTCTCCTTG 
GCGCTCCTGG GGCACATCCT GCTTCATGAC TTGGAGGTGG TCCCCCGAGA ACTACGAGGC 
TTCTCCCAAG ACGAGATTCA CCAAGCTTGC CAGCCAGGAG CCAGCAGCCC AGAATGCCGT 
GGCAGCCCCA GGGCAGCACC TACACAGTGC GACCTGCCCC CCAACAGCCG CTTTGACTGC 
GCCCCAGACA AGGGCATCAC CCCGCAGCAG TGTGAGGCCC GTGGCTGCTG CTACATGCCT 
GCAGAGTGGC CTCCGGATGC CCAGATGGGG CAGCCCTGGT GCTTCTTCCC TCCCAGCTAC 
CCCAGTTACA GGCTGGAGAA CCTGACCACC ACTGAGACAG GGTACACAGC CACCCTGACC 
CGTGCCGTCC CGACCTTCTT CCCCAAGGAC ATCATGACCT TGAGGCTGGA CATGTTGATG 
GAGACCGAGA GCCGACTCCA CTTCACGATC AAAGATCCTG CCAACAGACG CTATGAAGTG 
CCCTTGGAGA CCCCGCGTGT CTATAGCCAG GCGCCGTTCA CACTCTACAG CGTGGAGTTC 
TCGGAGGAGC CCTTTGGGGT GGTCGTGCGG CGGAAGCTGG ACGGACGGGT GCTGCTGAAC 
ACCACGGTGG CCCCCCTGTT CTTTGCGGAC CAGTTTCTGC AGCTGTCCAC CTCCCTGCCA 
TCCCAGCACA TCACAGGCCT TGCCGAACAC CTTGGGTCCC TGATGCTCAG CACCAACTGG 
ACCAAGATCA CCCTCTGGAA CCGGGACATC GCCCCTGAGC CCAACGTGAA CCTGTATGGA 
TCTCACCCTT TCTACCTGGT CCTGGAGGAT GGCGGGTTGG CTCACGGGGT CTTCCTGCTG 
AACAGCAATG CCATGGATGT GGTCCTGCAG CCCAGCCCAG CCCTCAGCTG GAGGTCGACA 
GGCGGGATCC TGGACGTGTA CATCTTCCTG GGCCCGGAGC CCAAGAGCGT GGTGCAGCAG 
TACCTGGACG TCGTGGGCTA CCCGTTCATG CCGCCGTATT GGGGCCTGGG CTTCCACCTG 
TGCCGCTGGG GCTACTCCAC CTCTGCCATC ACCCGCCAGG TCGTGGAGAA TATGACCAGG 
GCCTACTTCC CCCTGGACGT CCAGTGGAAT GACCTCGACT ACATGGATGC CAGACGGGAC 
TTCACTTTCA ACAAGGACCA CTTCGGGGAC TTCCCGGCCA TGGTGCAGGA GCTCCACCAG 
GGCGGTCGGC GGTACATCAT GATCGTGGAT CCTGCCATCA GCAGCTCAGG CCCTGCTGGG 
ACCTACCGAC CCTACGACGA GGGTCTGAGG CGGGGGGTCT TCATCACCAA TGAGACTGGG 
CAGCCACTGA TTGGGCAGGT GTGGCCCGGA CTCACCGCCT TCCCCGACTT CACCAACCCC 
GAAACTCTTG ACTGGTGGCA GGACATGGTG ACCGAGTTCC ACGCCCAAGT GCCCTTCGAT 
GGCATGTGGA TCGATATGAA CGAGCCATCC AATTTCGTGA GAGGCTCAGT GGATGGCTGC 
CCGGACAACA GCCTGGAGAA CCCGCCCTAC CTGCCAGGGG TGGTTGGCGG GACCCTCCGG 
GCAGCCACCA TCTGTGCCTC CAGCCACCAG TTCCTGTCCA CGCATTATGA CCTGCACAAC 
CTGTATGGCC TGACTGAAGC CTTAGCCTCC CACAGGGCCC TGGTGAAGGC TCGAGGGATG 
CGTCCCTTCG TGATCTCTCG CTCAACCTTT GCTGGCCACG GCCGATACTC CGGCCACTGG 
ACAGGGGATG TGTGGAGCAA CTGGGAGCAG CTTTCCTACT CCGTGCCAGA AATCCTGCTT 
TTCAATCTGC TGGGGGTGCC CCTGGTAGGG GCTGACATCT GTGGCTTCCT GGGCAACACC 
TCAGAGGAGC TGTGTGTGCG TTGGACCCAG CTGGGGGCCT TCTACCCCTT CATGCGGAAC 
CACAATGCCC TGAACAGCCA GCCGCAGGAA CCGTACAGGT TCAGCGAGAC GGCGCAGCAA 
GCCATGAGGA AGGCCTTCAC CCTGCGCTAC GTGCTACTGC CCTATCTCTA CACACTGTTC 
CACAGGGCCC ACGTCAGAGG CGAGACAGTG GCCCGGCCCC TCTTCCTGGA GTTCCCCGAG 
GACCCCAGCA CCTGGACTGT GGACCGCCAG CTCCTGTGGG GGGAGGCTCT GCTCATCACC 
CCGGTGCTCG AGGCTGAGAA GGTTGAAGTC ACTGGCTACT TCCCCCAGGG CACGTGGTAC 
GACCTGCAGA CGGTACCAAT GGAGGCCTTT GGCAGCCTCC CACCTCCTGC ACCCCTCACG 
TCTGTCATCC ACAGCAAGGG GCAGTGGGTG ACGCTGTCCG CCCCGCTGGA CACCATCAAC 
GTCCACCTCC GGGCCGGGCA CATCATCCCT ATGCAGGGCC CTGCCCTCAC GACCACAGAG 
TCCCGCAAGC AGCACATGGC CCTGGCTGTG GCCCTGACAG CCAGTGGGGA GGCCCAAGGG 
GAGCTGTTCT GGGACGACGG GGAGAGCCTG GGAGTGCTGG ATGGTGGGGA CTACACACAG 
CTCATCTTTC TGGCCAAGAA CAACACCTTT GTAAACAAGC TGGTGCACGT GAGCAGTGAG 
GGGGCCAGCC TGCAGCTGAG GAATGTGACC GTCCTGGGGG TGGCCACAGC CCCCCAGCAG 
GTCCTCTGCA ACAGCGTTCC TGTCTCCAAC TTCACCTTCA GCCCTGACAC AGAGACTCTG 
GCCATCCCTG TCTCGCTGAC CATGGGAGAG CAGTTTGTCA TCAGCTGGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The bovine alpha-glucosidase gene: coding region, genomic structure, and mutations that cause bovine generalized glycogenosis.
Mammalian genome : official journal of the International Mammalian Genome Society11(3)206-12(2000 Mar)
Dennis JA,Moran C,Healy PJ

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GAA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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