PDE8B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE8B
Entrez Gene ID	107131388
Full Name	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015473002.1	XP_015328488.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like
XM_024997900.1	XP_024853668.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X5
XM_015464952.1	XP_015320438.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like
XM_024997898.1	XP_024853666.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
XM_024997899.1	XP_024853667.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X4
XM_024997896.1	XP_024853664.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
XM_024997897.1	XP_024853665.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2

Pathways from BioSystems
Gene Ontology

The following PDE8B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE8B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa91590	XM_015464952.1 Latest version!	Bos taurus high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like (LOC107131388), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OBa91590	XM_015473002.1 Latest version!	Bos taurus high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like (LOC107131388), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OBa06274	XM_024997900.1 Latest version!	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa06275	XM_024997898.1 Latest version!	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa06276	XM_024997899.1 Latest version!	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa219769	XM_024997896.1 Latest version!	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa219770	XM_024997897.1 Latest version!	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE8B ( XM_015473002.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328488.1 Bos taurus high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like (LOC107131388), mRNA.

Price & Availability↑

CloneID	OBa91590
Clone ID Related Accession (Same CDS sequence)	XM_015473002.1 , XM_015464952.1
Accession Version	XM_015473002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTC CGAACGCCGG CCAGCAGGTT TCTTCTGCGG AGGTACGCAT CGGGCCCATG 
AGATTGACAC AGGATCCTAT CCAGGTTTTG CTCATCTTTG CAAAGGAAGA TAGTCAGAGT 
GATGGATTCT GGTGGGCCTG TGACAGAGCC GGTTACAGCT GCAATATTGC TCGGACTCCA 
GAGTCAGCCC TGGAATGCTT TCTAGATAAA CATCATGAGA TTATTGTAAT CGATCACAGA 
CAGACTCAAA ACTTCGATGC AGAAGCAGTG TGCAGGTCAA TCCGGGCTAC GAACCCCTCG 
GAGCACGCAG TGATCCTCGC CGTGGTCTCA CAAGCATCCG GCAACTATGA AGAGGCCTCT 
GTGCTCCCTC TTCTGCACGC GGGCTTCAAC AGGAGATTTA TGGAGAACAG CAGCATAATC 
GCTTGCTACA ATGAACTGAT TCAAATAGAA CATGGGGAAG TTCTCTCACA GTTCAAATTA 
AGGGCCTGTA ATTCAGTGTT TACAGCATTA GACCACTGTC ACGAAGCCAT AGAAATCACA 
AGCGATGACC ACGTGATCCA GTATGTCAAC CCAGCCTTCG AGAGGATGAT GGGCTACCAC 
AAAGGCGAAC TGCTGGGGAA AGAACTCGCC GAGCTGCCCA AAAGCGACAA GAACCGGGCA 
GACCTTCTCG ACACCATCAA TACCTGCATC AAGAAGGGCA AGGAGTGGCA GGGCGTCTAC 
TACGCCAGAC GGAAATCCGG CGACAGCATC CAGCAGCACG TGAAGATCAC ACCAGTGATC 
GGCCAAGGAG GGAAGATCAG GCATTTCGTC TCCCTCAAGA AACTGTGTTG TAGCACTGAC 
AAAAACAAGC AGCAGGGTCC TTGGGCTGTG CTTGCGTGCC TGTGCTGCCT GGCACCTAGT 
GGGTTCCCAA GCCGTCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328488.1
CDS	198..1118
Translation	MDPPNAGQQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYSCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHAVILAVVSQASGNYEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVLSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELAELPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCSTDKNKQQGPWAVLACLCCLAPSGFPSRR

Target ORF information:

RefSeq Version	XM_015473002.1
Organism	Bos taurus(cattle)
Definition	Bos taurus high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like (LOC107131388), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473002.1

ORF Insert Sequence:

ATGGACCCTC CGAACGCCGG CCAGCAGGTT TCTTCTGCGG AGGTACGCAT CGGGCCCATG 
AGATTGACAC AGGATCCTAT CCAGGTTTTG CTCATCTTTG CAAAGGAAGA TAGTCAGAGT 
GATGGATTCT GGTGGGCCTG TGACAGAGCC GGTTACAGCT GCAATATTGC TCGGACTCCA 
GAGTCAGCCC TGGAATGCTT TCTAGATAAA CATCATGAGA TTATTGTAAT CGATCACAGA 
CAGACTCAAA ACTTCGATGC AGAAGCAGTG TGCAGGTCAA TCCGGGCTAC GAACCCCTCG 
GAGCACGCAG TGATCCTCGC CGTGGTCTCA CAAGCATCCG GCAACTATGA AGAGGCCTCT 
GTGCTCCCTC TTCTGCACGC GGGCTTCAAC AGGAGATTTA TGGAGAACAG CAGCATAATC 
GCTTGCTACA ATGAACTGAT TCAAATAGAA CATGGGGAAG TTCTCTCACA GTTCAAATTA 
AGGGCCTGTA ATTCAGTGTT TACAGCATTA GACCACTGTC ACGAAGCCAT AGAAATCACA 
AGCGATGACC ACGTGATCCA GTATGTCAAC CCAGCCTTCG AGAGGATGAT GGGCTACCAC 
AAAGGCGAAC TGCTGGGGAA AGAACTCGCC GAGCTGCCCA AAAGCGACAA GAACCGGGCA 
GACCTTCTCG ACACCATCAA TACCTGCATC AAGAAGGGCA AGGAGTGGCA GGGCGTCTAC 
TACGCCAGAC GGAAATCCGG CGACAGCATC CAGCAGCACG TGAAGATCAC ACCAGTGATC 
GGCCAAGGAG GGAAGATCAG GCATTTCGTC TCCCTCAAGA AACTGTGTTG TAGCACTGAC 
AAAAACAAGC AGCAGGGTCC TTGGGCTGTG CTTGCGTGCC TGTGCTGCCT GGCACCTAGT 
GGGTTCCCAA GCCGTCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE8B ( XM_015464952.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320438.1 Bos taurus high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like (LOC107131388), mRNA.

Price & Availability↑

CloneID	OBa91590
Clone ID Related Accession (Same CDS sequence)	XM_015473002.1 , XM_015464952.1
Accession Version	XM_015464952.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645844.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTC CGAACGCCGG CCAGCAGGTT TCTTCTGCGG AGGTACGCAT CGGGCCCATG 
AGATTGACAC AGGATCCTAT CCAGGTTTTG CTCATCTTTG CAAAGGAAGA TAGTCAGAGT 
GATGGATTCT GGTGGGCCTG TGACAGAGCC GGTTACAGCT GCAATATTGC TCGGACTCCA 
GAGTCAGCCC TGGAATGCTT TCTAGATAAA CATCATGAGA TTATTGTAAT CGATCACAGA 
CAGACTCAAA ACTTCGATGC AGAAGCAGTG TGCAGGTCAA TCCGGGCTAC GAACCCCTCG 
GAGCACGCAG TGATCCTCGC CGTGGTCTCA CAAGCATCCG GCAACTATGA AGAGGCCTCT 
GTGCTCCCTC TTCTGCACGC GGGCTTCAAC AGGAGATTTA TGGAGAACAG CAGCATAATC 
GCTTGCTACA ATGAACTGAT TCAAATAGAA CATGGGGAAG TTCTCTCACA GTTCAAATTA 
AGGGCCTGTA ATTCAGTGTT TACAGCATTA GACCACTGTC ACGAAGCCAT AGAAATCACA 
AGCGATGACC ACGTGATCCA GTATGTCAAC CCAGCCTTCG AGAGGATGAT GGGCTACCAC 
AAAGGCGAAC TGCTGGGGAA AGAACTCGCC GAGCTGCCCA AAAGCGACAA GAACCGGGCA 
GACCTTCTCG ACACCATCAA TACCTGCATC AAGAAGGGCA AGGAGTGGCA GGGCGTCTAC 
TACGCCAGAC GGAAATCCGG CGACAGCATC CAGCAGCACG TGAAGATCAC ACCAGTGATC 
GGCCAAGGAG GGAAGATCAG GCATTTCGTC TCCCTCAAGA AACTGTGTTG TAGCACTGAC 
AAAAACAAGC AGCAGGGTCC TTGGGCTGTG CTTGCGTGCC TGTGCTGCCT GGCACCTAGT 
GGGTTCCCAA GCCGTCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320438.1
CDS	198..1118
Translation	MDPPNAGQQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYSCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHAVILAVVSQASGNYEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVLSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELAELPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCSTDKNKQQGPWAVLACLCCLAPSGFPSRR

Target ORF information:

RefSeq Version	XM_015464952.1
Organism	Bos taurus(cattle)
Definition	Bos taurus high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B-like (LOC107131388), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464952.1

ORF Insert Sequence:

ATGGACCCTC CGAACGCCGG CCAGCAGGTT TCTTCTGCGG AGGTACGCAT CGGGCCCATG 
AGATTGACAC AGGATCCTAT CCAGGTTTTG CTCATCTTTG CAAAGGAAGA TAGTCAGAGT 
GATGGATTCT GGTGGGCCTG TGACAGAGCC GGTTACAGCT GCAATATTGC TCGGACTCCA 
GAGTCAGCCC TGGAATGCTT TCTAGATAAA CATCATGAGA TTATTGTAAT CGATCACAGA 
CAGACTCAAA ACTTCGATGC AGAAGCAGTG TGCAGGTCAA TCCGGGCTAC GAACCCCTCG 
GAGCACGCAG TGATCCTCGC CGTGGTCTCA CAAGCATCCG GCAACTATGA AGAGGCCTCT 
GTGCTCCCTC TTCTGCACGC GGGCTTCAAC AGGAGATTTA TGGAGAACAG CAGCATAATC 
GCTTGCTACA ATGAACTGAT TCAAATAGAA CATGGGGAAG TTCTCTCACA GTTCAAATTA 
AGGGCCTGTA ATTCAGTGTT TACAGCATTA GACCACTGTC ACGAAGCCAT AGAAATCACA 
AGCGATGACC ACGTGATCCA GTATGTCAAC CCAGCCTTCG AGAGGATGAT GGGCTACCAC 
AAAGGCGAAC TGCTGGGGAA AGAACTCGCC GAGCTGCCCA AAAGCGACAA GAACCGGGCA 
GACCTTCTCG ACACCATCAA TACCTGCATC AAGAAGGGCA AGGAGTGGCA GGGCGTCTAC 
TACGCCAGAC GGAAATCCGG CGACAGCATC CAGCAGCACG TGAAGATCAC ACCAGTGATC 
GGCCAAGGAG GGAAGATCAG GCATTTCGTC TCCCTCAAGA AACTGTGTTG TAGCACTGAC 
AAAAACAAGC AGCAGGGTCC TTGGGCTGTG CTTGCGTGCC TGTGCTGCCT GGCACCTAGT 
GGGTTCCCAA GCCGTCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE8B ( XM_024997900.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853668.1 Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa06274
Clone ID Related Accession (Same CDS sequence)	XM_024997900.1
Accession Version	XM_024997900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTC CGAACGCCGG CCAGCAGGTT TCTTCTGCGG AGGTACGCAT CGGGCCCATG 
AGATTGACAC AGGATCCTAT CCAGGTTTTG CTCATCTTTG CAAAGGAAGA TAGTCAGAGT 
GATGGATTCT GGTGGGCCTG TGACAGAGCC GGTTACAGCT GCAATATTGC TCGGACTCCA 
GAGTCAGCCC TGGAATGCTT TCTAGATAAA CATCATGAGA TTATTGTAAT CGATCACAGA 
CAGACTCAAA ACTTCGATGC AGAAGCAGTG TGCAGGTCAA TCCGGGCTAC GAACCCCTCG 
GAGCACGCAG TGATCCTCGC CGTGGTCTCA CAAGCATCCG GCAACTATGA AGAGGCCTCT 
GTGCTCCCTC TTCTGCACGC GGGCTTCAAC AGGAGATTTA TGGAGAACAG CAGCATAATC 
GCTTGCTACA ATGAACTGAT TCAAATAGAA CATGGGGAAG TTCTCTCACA GTTCAAATTA 
AGGGCCTGTA ATTCAGTGTT TACAGCATTA GACCACTGTC ACGAAGCCAT AGAAATCACA 
AGCGATGACC ACGTGATCCA GTATGTCAAC CCAGCCTTCG AGAGGATGAT GGGCTACCAC 
AAAGGCGAAC TGCTGGGGAA AGAACTCGCC GAGCTGCCCA AAAGCGACAA GAACCGGGCA 
GACCTTCTCG ACACCATCAA TACCTGCATC AAGAAGGGCA AGGAGTGGCA GGGCGTCTAC 
TACGCCAGAC GGAAATCCGG CGACAGCATC CAGCAGCACG TGAAGATCAC ACCAGTGATC 
GGCCAAGGAG GGAAGATCAG GCATTTCGTC TCCCTCAAGA AACTGTGTTG TAGCACTGAC 
AAAAACAAGC AGATTCTCAA GATTCATCGT GATTCAGGAG ACAATTCTCA GACAGAGTCT 
CATTCATTCA GATACAAGAA CAGGAGGAAA GAGTCCATTG ATGTGAAATC AATATCATCT 
CGAGGCAGTG ATGCACCAAG TCTGCAGAAC CGTCGCTACC CATCCATGGC GCGGATCCAC 
TCCATGACCA TTGAGGCTCC CATCACGAAG GTTATAAATA TAATCAACGC AGCCCAAGAA 
AACAGTCCTG TCACAGTGGC AGAAGCCTTG GACAGAGTTT TAGAAATTTT ACGAACTACG 
GAACTGTACT CCCCTCAGCT CGGTACCAAA GATGAAGATC CCCACACCAG CGACCTCGTG 
GGAGGGCTGA TGACTGATGG CTTGAGAAGG TTGTCAGGAA ACGAGTACGT GTTCACTAAG 
AACGTACACC AGAGTCATAG TCACCTTGCA ATGCCAGGAA CCATCACCGA CGTGCCCCCT 
TCCGTCGCCC AACTACTTGA CAATGAGGAA AGCTGGGAGT TCAACATCTT TGAACTGGAA 
GCTGTGACGC ACAAAAGGCC ATTGGTTTAC CTGGGCTTGA AAGTTTTCTC CCGGTTTGGA 
GTATGTGAAT TCCTACACTG TTCTGAAACT ACTCTTCGGG CCTGGCTCCA GGTGATCGAA 
GCCAACTACC ACTCTTCCAA CGCTTACCAC AACTCCACCC ACGCCGCTGA CGTCCTGCAC 
GCCACAGCCT TCTTCCTCGG CAAGGAGAGA GTGAAGGGAA GCTTGGATCA GCTGGACGAG 
GTGGCCGCCC TGATCGCTGC CACAGTCCAC GACGTGGACC ACCCGGGAAG GACCAACTCT 
TTCCTGTGCA ACGCGGGCAG TGAGCTCGCT GTGCTCTATA ATGACACCGC TGTCTTGGAG 
AGTCACCACA CAGCCCTGGC CTTCCAGCTC ACGGTCAAGG ACAGCAAATG CAACATTTTC 
AAGAATATCG ACAGGAACCA TTATCGAACA CTGCGCCAAG CTATTATCGA CATGGTTTTA 
GCAACAGAGA TGACAAAACA CTTTGAACAT GTGAACAAAT TTGTGAACAG CATTAACAAG 
CCAATGGCAG CTGAGATTGA CGGCAGCGAC TGCGAGTGCA GCCCTCCTGG GAAGAACTTC 
CCTGAGAACC AGACCCTGAT CAAGCGCATG ATGATTAAGT GTGCGGATGT AGCCAACCCC 
TGCCGCCCCC TGGACCTGTG CATTGAATGG GCTGGGAGGA TCTCTGAGGA GTACTTTGCG 
CAGACGGATG AAGAGAAGAG ACAGGGGCTA CCCGTAGTGA TGCCAGTGTT TGACCGGAAT 
ACCTGTAGCA TCCCCAAGTC CCAAATCTCC TTTATTGACT ACTTCATAAC AGACATGTTT 
GATGCTTGGG ATGCCTTTGC ACACCTGCCA ACGCTGATGC AACATTTGGC TGATAACTAC 
AAACACTGGA AGACATTAGA TGACCTAAAG TGCAAAAGTC TGAGGCTTCC ATCTGACAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853668.1
CDS	197..2539
Translation	MDPPNAGQQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYSCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHAVILAVVSQASGNYEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVLSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELAELPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCSTDKNKQILKIHRDSGDNSQTESHSFRYKNRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHQSHSHLAMPGTITDVPPSVAQLLDNEESWEFNIFELEAVTHKRPLVYLGLKVFSRFGVCEFLHCSETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDSKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPMAAEIDGSDCECSPPGKNFPENQTLIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPTLMQHLADNYKHWKTLDDLKCKSLRLPSDS

Target ORF information:

RefSeq Version	XM_024997900.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997900.1

ORF Insert Sequence:

ATGGACCCTC CGAACGCCGG CCAGCAGGTT TCTTCTGCGG AGGTACGCAT CGGGCCCATG 
AGATTGACAC AGGATCCTAT CCAGGTTTTG CTCATCTTTG CAAAGGAAGA TAGTCAGAGT 
GATGGATTCT GGTGGGCCTG TGACAGAGCC GGTTACAGCT GCAATATTGC TCGGACTCCA 
GAGTCAGCCC TGGAATGCTT TCTAGATAAA CATCATGAGA TTATTGTAAT CGATCACAGA 
CAGACTCAAA ACTTCGATGC AGAAGCAGTG TGCAGGTCAA TCCGGGCTAC GAACCCCTCG 
GAGCACGCAG TGATCCTCGC CGTGGTCTCA CAAGCATCCG GCAACTATGA AGAGGCCTCT 
GTGCTCCCTC TTCTGCACGC GGGCTTCAAC AGGAGATTTA TGGAGAACAG CAGCATAATC 
GCTTGCTACA ATGAACTGAT TCAAATAGAA CATGGGGAAG TTCTCTCACA GTTCAAATTA 
AGGGCCTGTA ATTCAGTGTT TACAGCATTA GACCACTGTC ACGAAGCCAT AGAAATCACA 
AGCGATGACC ACGTGATCCA GTATGTCAAC CCAGCCTTCG AGAGGATGAT GGGCTACCAC 
AAAGGCGAAC TGCTGGGGAA AGAACTCGCC GAGCTGCCCA AAAGCGACAA GAACCGGGCA 
GACCTTCTCG ACACCATCAA TACCTGCATC AAGAAGGGCA AGGAGTGGCA GGGCGTCTAC 
TACGCCAGAC GGAAATCCGG CGACAGCATC CAGCAGCACG TGAAGATCAC ACCAGTGATC 
GGCCAAGGAG GGAAGATCAG GCATTTCGTC TCCCTCAAGA AACTGTGTTG TAGCACTGAC 
AAAAACAAGC AGATTCTCAA GATTCATCGT GATTCAGGAG ACAATTCTCA GACAGAGTCT 
CATTCATTCA GATACAAGAA CAGGAGGAAA GAGTCCATTG ATGTGAAATC AATATCATCT 
CGAGGCAGTG ATGCACCAAG TCTGCAGAAC CGTCGCTACC CATCCATGGC GCGGATCCAC 
TCCATGACCA TTGAGGCTCC CATCACGAAG GTTATAAATA TAATCAACGC AGCCCAAGAA 
AACAGTCCTG TCACAGTGGC AGAAGCCTTG GACAGAGTTT TAGAAATTTT ACGAACTACG 
GAACTGTACT CCCCTCAGCT CGGTACCAAA GATGAAGATC CCCACACCAG CGACCTCGTG 
GGAGGGCTGA TGACTGATGG CTTGAGAAGG TTGTCAGGAA ACGAGTACGT GTTCACTAAG 
AACGTACACC AGAGTCATAG TCACCTTGCA ATGCCAGGAA CCATCACCGA CGTGCCCCCT 
TCCGTCGCCC AACTACTTGA CAATGAGGAA AGCTGGGAGT TCAACATCTT TGAACTGGAA 
GCTGTGACGC ACAAAAGGCC ATTGGTTTAC CTGGGCTTGA AAGTTTTCTC CCGGTTTGGA 
GTATGTGAAT TCCTACACTG TTCTGAAACT ACTCTTCGGG CCTGGCTCCA GGTGATCGAA 
GCCAACTACC ACTCTTCCAA CGCTTACCAC AACTCCACCC ACGCCGCTGA CGTCCTGCAC 
GCCACAGCCT TCTTCCTCGG CAAGGAGAGA GTGAAGGGAA GCTTGGATCA GCTGGACGAG 
GTGGCCGCCC TGATCGCTGC CACAGTCCAC GACGTGGACC ACCCGGGAAG GACCAACTCT 
TTCCTGTGCA ACGCGGGCAG TGAGCTCGCT GTGCTCTATA ATGACACCGC TGTCTTGGAG 
AGTCACCACA CAGCCCTGGC CTTCCAGCTC ACGGTCAAGG ACAGCAAATG CAACATTTTC 
AAGAATATCG ACAGGAACCA TTATCGAACA CTGCGCCAAG CTATTATCGA CATGGTTTTA 
GCAACAGAGA TGACAAAACA CTTTGAACAT GTGAACAAAT TTGTGAACAG CATTAACAAG 
CCAATGGCAG CTGAGATTGA CGGCAGCGAC TGCGAGTGCA GCCCTCCTGG GAAGAACTTC 
CCTGAGAACC AGACCCTGAT CAAGCGCATG ATGATTAAGT GTGCGGATGT AGCCAACCCC 
TGCCGCCCCC TGGACCTGTG CATTGAATGG GCTGGGAGGA TCTCTGAGGA GTACTTTGCG 
CAGACGGATG AAGAGAAGAG ACAGGGGCTA CCCGTAGTGA TGCCAGTGTT TGACCGGAAT 
ACCTGTAGCA TCCCCAAGTC CCAAATCTCC TTTATTGACT ACTTCATAAC AGACATGTTT 
GATGCTTGGG ATGCCTTTGC ACACCTGCCA ACGCTGATGC AACATTTGGC TGATAACTAC 
AAACACTGGA AGACATTAGA TGACCTAAAG TGCAAAAGTC TGAGGCTTCC ATCTGACAGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE8B ( XM_024997898.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853666.1 Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa06275
Clone ID Related Accession (Same CDS sequence)	XM_024997898.1
Accession Version	XM_024997898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCTC CGAACGCCGG CCAGGTTTCT TCTGCGGAGG TACGCATCGG GCCCATGAGA 
TTGACACAGG ATCCTATCCA GGTTTTGCTC ATCTTTGCAA AGGAAGATAG TCAGAGTGAT 
GGATTCTGGT GGGCCTGTGA CAGAGCCGGT TACAGCTGCA ATATTGCTCG GACTCCAGAG 
TCAGCCCTGG AATGCTTTCT AGATAAACAT CATGAGATTA TTGTAATCGA TCACAGACAG 
ACTCAAAACT TCGATGCAGA AGCAGTGTGC AGGTCAATCC GGGCTACGAA CCCCTCGGAG 
CACGCAGTGA TCCTCGCCGT GGTCTCACAA GCATCCGGCA ACTATGAAGA GGCCTCTGTG 
CTCCCTCTTC TGCACGCGGG CTTCAACAGG AGATTTATGG AGAACAGCAG CATAATCGCT 
TGCTACAATG AACTGATTCA AATAGAACAT GGGGAAGTTC TCTCACAGTT CAAATTAAGG 
GCCTGTAATT CAGTGTTTAC AGCATTAGAC CACTGTCACG AAGCCATAGA AATCACAAGC 
GATGACCACG TGATCCAGTA TGTCAACCCA GCCTTCGAGA GGATGATGGG CTACCACAAA 
GGCGAACTGC TGGGGAAAGA ACTCGCCGAG CTGCCCAAAA GCGACAAGAA CCGGGCAGAC 
CTTCTCGACA CCATCAATAC CTGCATCAAG AAGGGCAAGG AGTGGCAGGG CGTCTACTAC 
GCCAGACGGA AATCCGGCGA CAGCATCCAG CAGCACGTGA AGATCACACC AGTGATCGGC 
CAAGGAGGGA AGATCAGGCA TTTCGTCTCC CTCAAGAAAC TGTGTTGTAG CACTGACAAA 
AACAAGCAGA TTCTCAAGAT TCATCGTGAT TCAGGAGACA ATTCTCAGAC AGAGTCTCAT 
TCATTCAGAT ACAAGAACAG GAGGAAAGAG TCCATTGATG TGAAATCAAT ATCATCTCGA 
GGCAGTGATG CACCAAGTCT GCAGAACCGT CGCTACCCAT CCATGGCGCG GATCCACTCC 
ATGACCATTG AGGCTCCCAT CACGAAGGTT ATAAATATAA TCAACGCAGC CCAAGAAAAC 
AGTCCTGTCA CAGTGGCAGA AGCCTTGGAC AGAGTTTTAG AAATTTTACG AACTACGGAA 
CTGTACTCCC CTCAGCTCGG TACCAAAGAT GAAGATCCCC ACACCAGCGA CCTCGTGGGA 
GGGCTGATGA CTGATGGCTT GAGAAGGTTG TCAGGAAACG AGTACGTGTT CACTAAGAAC 
GTACACCAGA GTCATAGTCA CCTTGCAATG CCAGGAACCA TCACCGACGT GCCCCCTTCC 
GTCGCCCAAC TACTTGACAA TGAGGAAAGC TGGGAGTTCA ACATCTTTGA ACTGGAAGCT 
GTGACGCACA AAAGGCCATT GGTTTACCTG GGCTTGAAAG TTTTCTCCCG GTTTGGAGTA 
TGTGAATTCC TACACTGTTC TGAAACTACT CTTCGGGCCT GGCTCCAGGT GATCGAAGCC 
AACTACCACT CTTCCAACGC TTACCACAAC TCCACCCACG CCGCTGACGT CCTGCACGCC 
ACAGCCTTCT TCCTCGGCAA GGAGAGAGTG AAGGGAAGCT TGGATCAGCT GGACGAGGTG 
GCCGCCCTGA TCGCTGCCAC AGTCCACGAC GTGGACCACC CGGGAAGGAC CAACTCTTTC 
CTGTGCAACG CGGGCAGTGA GCTCGCTGTG CTCTATAATG ACACCGCTGT CTTGGAGAGT 
CACCACACAG CCCTGGCCTT CCAGCTCACG GTCAAGGACA GCAAATGCAA CATTTTCAAG 
AATATCGACA GGAACCATTA TCGAACACTG CGCCAAGCTA TTATCGACAT GGTTTTAGCA 
ACAGAGATGA CAAAACACTT TGAACATGTG AACAAATTTG TGAACAGCAT TAACAAGCCA 
ATGGCAGCTG AGATTGACGG CAGCGACTGC GAGTGCAGCC CTCCTGGGAA GAACTTCCCT 
GAGAACCAGA CCCTGATCAA GCGCATGATG ATTAAGTGTG CGGATGTAGC CAACCCCTGC 
CGCCCCCTGG ACCTGTGCAT TGAATGGGCT GGGAGGATCT CTGAGGAGTA CTTTGCGCAG 
ACGGATGAAG AGAAGAGACA GGGGCTACCC GTAGTGATGC CAGTGTTTGA CCGGAATACC 
TGTAGCATCC CCAAGTCCCA AATCTCCTTT ATTGACTACT TCATAACAGA CATGTTTGAT 
GCTTGGGATG CCTTTGCACA CCTGCCAACG CTGATGCAAC ATTTGGCTGA TAACTACAAA 
CACTGGAAGA CATTAGATGA CCTAAAGTGC AAAAGTCTGA GGCTTCCATC TGACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853666.1
CDS	146..2485
Translation	MDPPNAGQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYSCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHAVILAVVSQASGNYEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVLSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELAELPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCSTDKNKQILKIHRDSGDNSQTESHSFRYKNRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHQSHSHLAMPGTITDVPPSVAQLLDNEESWEFNIFELEAVTHKRPLVYLGLKVFSRFGVCEFLHCSETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDSKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPMAAEIDGSDCECSPPGKNFPENQTLIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPTLMQHLADNYKHWKTLDDLKCKSLRLPSDS

Target ORF information:

RefSeq Version	XM_024997898.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997898.1

ORF Insert Sequence:

ATGGACCCTC CGAACGCCGG CCAGGTTTCT TCTGCGGAGG TACGCATCGG GCCCATGAGA 
TTGACACAGG ATCCTATCCA GGTTTTGCTC ATCTTTGCAA AGGAAGATAG TCAGAGTGAT 
GGATTCTGGT GGGCCTGTGA CAGAGCCGGT TACAGCTGCA ATATTGCTCG GACTCCAGAG 
TCAGCCCTGG AATGCTTTCT AGATAAACAT CATGAGATTA TTGTAATCGA TCACAGACAG 
ACTCAAAACT TCGATGCAGA AGCAGTGTGC AGGTCAATCC GGGCTACGAA CCCCTCGGAG 
CACGCAGTGA TCCTCGCCGT GGTCTCACAA GCATCCGGCA ACTATGAAGA GGCCTCTGTG 
CTCCCTCTTC TGCACGCGGG CTTCAACAGG AGATTTATGG AGAACAGCAG CATAATCGCT 
TGCTACAATG AACTGATTCA AATAGAACAT GGGGAAGTTC TCTCACAGTT CAAATTAAGG 
GCCTGTAATT CAGTGTTTAC AGCATTAGAC CACTGTCACG AAGCCATAGA AATCACAAGC 
GATGACCACG TGATCCAGTA TGTCAACCCA GCCTTCGAGA GGATGATGGG CTACCACAAA 
GGCGAACTGC TGGGGAAAGA ACTCGCCGAG CTGCCCAAAA GCGACAAGAA CCGGGCAGAC 
CTTCTCGACA CCATCAATAC CTGCATCAAG AAGGGCAAGG AGTGGCAGGG CGTCTACTAC 
GCCAGACGGA AATCCGGCGA CAGCATCCAG CAGCACGTGA AGATCACACC AGTGATCGGC 
CAAGGAGGGA AGATCAGGCA TTTCGTCTCC CTCAAGAAAC TGTGTTGTAG CACTGACAAA 
AACAAGCAGA TTCTCAAGAT TCATCGTGAT TCAGGAGACA ATTCTCAGAC AGAGTCTCAT 
TCATTCAGAT ACAAGAACAG GAGGAAAGAG TCCATTGATG TGAAATCAAT ATCATCTCGA 
GGCAGTGATG CACCAAGTCT GCAGAACCGT CGCTACCCAT CCATGGCGCG GATCCACTCC 
ATGACCATTG AGGCTCCCAT CACGAAGGTT ATAAATATAA TCAACGCAGC CCAAGAAAAC 
AGTCCTGTCA CAGTGGCAGA AGCCTTGGAC AGAGTTTTAG AAATTTTACG AACTACGGAA 
CTGTACTCCC CTCAGCTCGG TACCAAAGAT GAAGATCCCC ACACCAGCGA CCTCGTGGGA 
GGGCTGATGA CTGATGGCTT GAGAAGGTTG TCAGGAAACG AGTACGTGTT CACTAAGAAC 
GTACACCAGA GTCATAGTCA CCTTGCAATG CCAGGAACCA TCACCGACGT GCCCCCTTCC 
GTCGCCCAAC TACTTGACAA TGAGGAAAGC TGGGAGTTCA ACATCTTTGA ACTGGAAGCT 
GTGACGCACA AAAGGCCATT GGTTTACCTG GGCTTGAAAG TTTTCTCCCG GTTTGGAGTA 
TGTGAATTCC TACACTGTTC TGAAACTACT CTTCGGGCCT GGCTCCAGGT GATCGAAGCC 
AACTACCACT CTTCCAACGC TTACCACAAC TCCACCCACG CCGCTGACGT CCTGCACGCC 
ACAGCCTTCT TCCTCGGCAA GGAGAGAGTG AAGGGAAGCT TGGATCAGCT GGACGAGGTG 
GCCGCCCTGA TCGCTGCCAC AGTCCACGAC GTGGACCACC CGGGAAGGAC CAACTCTTTC 
CTGTGCAACG CGGGCAGTGA GCTCGCTGTG CTCTATAATG ACACCGCTGT CTTGGAGAGT 
CACCACACAG CCCTGGCCTT CCAGCTCACG GTCAAGGACA GCAAATGCAA CATTTTCAAG 
AATATCGACA GGAACCATTA TCGAACACTG CGCCAAGCTA TTATCGACAT GGTTTTAGCA 
ACAGAGATGA CAAAACACTT TGAACATGTG AACAAATTTG TGAACAGCAT TAACAAGCCA 
ATGGCAGCTG AGATTGACGG CAGCGACTGC GAGTGCAGCC CTCCTGGGAA GAACTTCCCT 
GAGAACCAGA CCCTGATCAA GCGCATGATG ATTAAGTGTG CGGATGTAGC CAACCCCTGC 
CGCCCCCTGG ACCTGTGCAT TGAATGGGCT GGGAGGATCT CTGAGGAGTA CTTTGCGCAG 
ACGGATGAAG AGAAGAGACA GGGGCTACCC GTAGTGATGC CAGTGTTTGA CCGGAATACC 
TGTAGCATCC CCAAGTCCCA AATCTCCTTT ATTGACTACT TCATAACAGA CATGTTTGAT 
GCTTGGGATG CCTTTGCACA CCTGCCAACG CTGATGCAAC ATTTGGCTGA TAACTACAAA 
CACTGGAAGA CATTAGATGA CCTAAAGTGC AAAAGTCTGA GGCTTCCATC TGACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE8B ( XM_024997899.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853667.1 Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa06276
Clone ID Related Accession (Same CDS sequence)	XM_024997899.1
Accession Version	XM_024997899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGG TTTCTTCTGC GGAGGTACGC ATCGGGCCCA TGAGATTGAC ACAGGATCCT 
ATCCAGGTTT TGCTCATCTT TGCAAAGGAA GATAGTCAGA GTGATGGATT CTGGTGGGCC 
TGTGACAGAG CCGGTTACAG CTGCAATATT GCTCGGACTC CAGAGTCAGC CCTGGAATGC 
TTTCTAGATA AACATCATGA GATTATTGTA ATCGATCACA GACAGACTCA AAACTTCGAT 
GCAGAAGCAG TGTGCAGGTC AATCCGGGCT ACGAACCCCT CGGAGCACGC AGTGATCCTC 
GCCGTGGTCT CACAAGCATC CGGCAACTAT GAAGAGGCCT CTGTGCTCCC TCTTCTGCAC 
GCGGGCTTCA ACAGGAGATT TATGGAGAAC AGCAGCATAA TCGCTTGCTA CAATGAACTG 
ATTCAAATAG AACATGGGGA AGTTCTCTCA CAGTTCAAAT TAAGGGCCTG TAATTCAGTG 
TTTACAGCAT TAGACCACTG TCACGAAGCC ATAGAAATCA CAAGCGATGA CCACGTGATC 
CAGTATGTCA ACCCAGCCTT CGAGAGGATG ATGGGCTACC ACAAAGGCGA ACTGCTGGGG 
AAAGAACTCG CCGAGCTGCC CAAAAGCGAC AAGAACCGGG CAGACCTTCT CGACACCATC 
AATACCTGCA TCAAGAAGGG CAAGGAGTGG CAGGGCGTCT ACTACGCCAG ACGGAAATCC 
GGCGACAGCA TCCAGCAGCA CGTGAAGATC ACACCAGTGA TCGGCCAAGG AGGGAAGATC 
AGGCATTTCG TCTCCCTCAA GAAACTGTGT TGTAGCACTG ACAAAAACAA GCAGATTCTC 
AAGATTCATC GTGATTCAGG AGACAATTCT CAGACAGAGT CTCATTCATT CAGATACAAG 
AACAGGAGGA AAGAGTCCAT TGATGTGAAA TCAATATCAT CTCGAGGCAG TGATGCACCA 
AGTCTGCAGA ACCGTCGCTA CCCATCCATG GCGCGGATCC ACTCCATGAC CATTGAGGCT 
CCCATCACGA AGGTTATAAA TATAATCAAC GCAGCCCAAG AAAACAGTCC TGTCACAGTG 
GCAGAAGCCT TGGACAGAGT TTTAGAAATT TTACGAACTA CGGAACTGTA CTCCCCTCAG 
CTCGGTACCA AAGATGAAGA TCCCCACACC AGCGACCTCG TGGGAGGGCT GATGACTGAT 
GGCTTGAGAA GGTTGTCAGG AAACGAGTAC GTGTTCACTA AGAACGTACA CCAGAGTCAT 
AGTCACCTTG CAATGCCAGG AACCATCACC GACGTGCCCC CTTCCGTCGC CCAACTACTT 
GACAATGAGG AAAGCTGGGA GTTCAACATC TTTGAACTGG AAGCTGTGAC GCACAAAAGG 
CCATTGGTTT ACCTGGGCTT GAAAGTTTTC TCCCGGTTTG GAGTATGTGA ATTCCTACAC 
TGTTCTGAAA CTACTCTTCG GGCCTGGCTC CAGGTGATCG AAGCCAACTA CCACTCTTCC 
AACGCTTACC ACAACTCCAC CCACGCCGCT GACGTCCTGC ACGCCACAGC CTTCTTCCTC 
GGCAAGGAGA GAGTGAAGGG AAGCTTGGAT CAGCTGGACG AGGTGGCCGC CCTGATCGCT 
GCCACAGTCC ACGACGTGGA CCACCCGGGA AGGACCAACT CTTTCCTGTG CAACGCGGGC 
AGTGAGCTCG CTGTGCTCTA TAATGACACC GCTGTCTTGG AGAGTCACCA CACAGCCCTG 
GCCTTCCAGC TCACGGTCAA GGACAGCAAA TGCAACATTT TCAAGAATAT CGACAGGAAC 
CATTATCGAA CACTGCGCCA AGCTATTATC GACATGGTTT TAGCAACAGA GATGACAAAA 
CACTTTGAAC ATGTGAACAA ATTTGTGAAC AGCATTAACA AGCCAATGGC AGCTGAGATT 
GACGGCAGCG ACTGCGAGTG CAGCCCTCCT GGGAAGAACT TCCCTGAGAA CCAGACCCTG 
ATCAAGCGCA TGATGATTAA GTGTGCGGAT GTAGCCAACC CCTGCCGCCC CCTGGACCTG 
TGCATTGAAT GGGCTGGGAG GATCTCTGAG GAGTACTTTG CGCAGACGGA TGAAGAGAAG 
AGACAGGGGC TACCCGTAGT GATGCCAGTG TTTGACCGGA ATACCTGTAG CATCCCCAAG 
TCCCAAATCT CCTTTATTGA CTACTTCATA ACAGACATGT TTGATGCTTG GGATGCCTTT 
GCACACCTGC CAACGCTGAT GCAACATTTG GCTGATAACT ACAAACACTG GAAGACATTA 
GATGACCTAA AGTGCAAAAG TCTGAGGCTT CCATCTGACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853667.1
CDS	16..2340
Translation	MQQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYSCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHAVILAVVSQASGNYEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVLSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELAELPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCSTDKNKQILKIHRDSGDNSQTESHSFRYKNRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHQSHSHLAMPGTITDVPPSVAQLLDNEESWEFNIFELEAVTHKRPLVYLGLKVFSRFGVCEFLHCSETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDSKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPMAAEIDGSDCECSPPGKNFPENQTLIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPTLMQHLADNYKHWKTLDDLKCKSLRLPSDS

Target ORF information:

RefSeq Version	XM_024997899.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997899.1

ORF Insert Sequence:

ATGCAGCAGG TTTCTTCTGC GGAGGTACGC ATCGGGCCCA TGAGATTGAC ACAGGATCCT 
ATCCAGGTTT TGCTCATCTT TGCAAAGGAA GATAGTCAGA GTGATGGATT CTGGTGGGCC 
TGTGACAGAG CCGGTTACAG CTGCAATATT GCTCGGACTC CAGAGTCAGC CCTGGAATGC 
TTTCTAGATA AACATCATGA GATTATTGTA ATCGATCACA GACAGACTCA AAACTTCGAT 
GCAGAAGCAG TGTGCAGGTC AATCCGGGCT ACGAACCCCT CGGAGCACGC AGTGATCCTC 
GCCGTGGTCT CACAAGCATC CGGCAACTAT GAAGAGGCCT CTGTGCTCCC TCTTCTGCAC 
GCGGGCTTCA ACAGGAGATT TATGGAGAAC AGCAGCATAA TCGCTTGCTA CAATGAACTG 
ATTCAAATAG AACATGGGGA AGTTCTCTCA CAGTTCAAAT TAAGGGCCTG TAATTCAGTG 
TTTACAGCAT TAGACCACTG TCACGAAGCC ATAGAAATCA CAAGCGATGA CCACGTGATC 
CAGTATGTCA ACCCAGCCTT CGAGAGGATG ATGGGCTACC ACAAAGGCGA ACTGCTGGGG 
AAAGAACTCG CCGAGCTGCC CAAAAGCGAC AAGAACCGGG CAGACCTTCT CGACACCATC 
AATACCTGCA TCAAGAAGGG CAAGGAGTGG CAGGGCGTCT ACTACGCCAG ACGGAAATCC 
GGCGACAGCA TCCAGCAGCA CGTGAAGATC ACACCAGTGA TCGGCCAAGG AGGGAAGATC 
AGGCATTTCG TCTCCCTCAA GAAACTGTGT TGTAGCACTG ACAAAAACAA GCAGATTCTC 
AAGATTCATC GTGATTCAGG AGACAATTCT CAGACAGAGT CTCATTCATT CAGATACAAG 
AACAGGAGGA AAGAGTCCAT TGATGTGAAA TCAATATCAT CTCGAGGCAG TGATGCACCA 
AGTCTGCAGA ACCGTCGCTA CCCATCCATG GCGCGGATCC ACTCCATGAC CATTGAGGCT 
CCCATCACGA AGGTTATAAA TATAATCAAC GCAGCCCAAG AAAACAGTCC TGTCACAGTG 
GCAGAAGCCT TGGACAGAGT TTTAGAAATT TTACGAACTA CGGAACTGTA CTCCCCTCAG 
CTCGGTACCA AAGATGAAGA TCCCCACACC AGCGACCTCG TGGGAGGGCT GATGACTGAT 
GGCTTGAGAA GGTTGTCAGG AAACGAGTAC GTGTTCACTA AGAACGTACA CCAGAGTCAT 
AGTCACCTTG CAATGCCAGG AACCATCACC GACGTGCCCC CTTCCGTCGC CCAACTACTT 
GACAATGAGG AAAGCTGGGA GTTCAACATC TTTGAACTGG AAGCTGTGAC GCACAAAAGG 
CCATTGGTTT ACCTGGGCTT GAAAGTTTTC TCCCGGTTTG GAGTATGTGA ATTCCTACAC 
TGTTCTGAAA CTACTCTTCG GGCCTGGCTC CAGGTGATCG AAGCCAACTA CCACTCTTCC 
AACGCTTACC ACAACTCCAC CCACGCCGCT GACGTCCTGC ACGCCACAGC CTTCTTCCTC 
GGCAAGGAGA GAGTGAAGGG AAGCTTGGAT CAGCTGGACG AGGTGGCCGC CCTGATCGCT 
GCCACAGTCC ACGACGTGGA CCACCCGGGA AGGACCAACT CTTTCCTGTG CAACGCGGGC 
AGTGAGCTCG CTGTGCTCTA TAATGACACC GCTGTCTTGG AGAGTCACCA CACAGCCCTG 
GCCTTCCAGC TCACGGTCAA GGACAGCAAA TGCAACATTT TCAAGAATAT CGACAGGAAC 
CATTATCGAA CACTGCGCCA AGCTATTATC GACATGGTTT TAGCAACAGA GATGACAAAA 
CACTTTGAAC ATGTGAACAA ATTTGTGAAC AGCATTAACA AGCCAATGGC AGCTGAGATT 
GACGGCAGCG ACTGCGAGTG CAGCCCTCCT GGGAAGAACT TCCCTGAGAA CCAGACCCTG 
ATCAAGCGCA TGATGATTAA GTGTGCGGAT GTAGCCAACC CCTGCCGCCC CCTGGACCTG 
TGCATTGAAT GGGCTGGGAG GATCTCTGAG GAGTACTTTG CGCAGACGGA TGAAGAGAAG 
AGACAGGGGC TACCCGTAGT GATGCCAGTG TTTGACCGGA ATACCTGTAG CATCCCCAAG 
TCCCAAATCT CCTTTATTGA CTACTTCATA ACAGACATGT TTGATGCTTG GGATGCCTTT 
GCACACCTGC CAACGCTGAT GCAACATTTG GCTGATAACT ACAAACACTG GAAGACATTA 
GATGACCTAA AGTGCAAAAG TCTGAGGCTT CCATCTGACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE8B ( XM_024997896.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853664.1 Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219769
Clone ID Related Accession (Same CDS sequence)	XM_024997896.1
Accession Version	XM_024997896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CCCCCAGCAT CCACGTCTCG CAGAGCGGCG TGATCTACTG CCGGGACTCG 
GACGAGTCCA ACTCGCCCCG CCAGACCACC GGCGTGTCGC AGGGCCCCGC GGGCCCCCTG 
CACGGCCTCT TCGTCCAGAC CGACGCCGCC GACGCCATCC CCCAGAGCCG CGCGTCCGGG 
TCCCCAGGCG CCGCCCGCGC CCGCAGGGCC CGCGGGGAGC TGGGCAGCGG CAGCAGCGCG 
GGCTCCGCGG GCCCCGCGGC GACCACCTGC AGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCGGAGGCCG AGACCCAGAC CAGCTACACC AGCGTCAAGC AGGTTTCTTC TGCGGAGGTA 
CGCATCGGGC CCATGAGATT GACACAGGAT CCTATCCAGG TTTTGCTCAT CTTTGCAAAG 
GAAGATAGTC AGAGTGATGG ATTCTGGTGG GCCTGTGACA GAGCCGGTTA CAGCTGCAAT 
ATTGCTCGGA CTCCAGAGTC AGCCCTGGAA TGCTTTCTAG ATAAACATCA TGAGATTATT 
GTAATCGATC ACAGACAGAC TCAAAACTTC GATGCAGAAG CAGTGTGCAG GTCAATCCGG 
GCTACGAACC CCTCGGAGCA CGCAGTGATC CTCGCCGTGG TCTCACAAGC ATCCGGCAAC 
TATGAAGAGG CCTCTGTGCT CCCTCTTCTG CACGCGGGCT TCAACAGGAG ATTTATGGAG 
AACAGCAGCA TAATCGCTTG CTACAATGAA CTGATTCAAA TAGAACATGG GGAAGTTCTC 
TCACAGTTCA AATTAAGGGC CTGTAATTCA GTGTTTACAG CATTAGACCA CTGTCACGAA 
GCCATAGAAA TCACAAGCGA TGACCACGTG ATCCAGTATG TCAACCCAGC CTTCGAGAGG 
ATGATGGGCT ACCACAAAGG CGAACTGCTG GGGAAAGAAC TCGCCGAGCT GCCCAAAAGC 
GACAAGAACC GGGCAGACCT TCTCGACACC ATCAATACCT GCATCAAGAA GGGCAAGGAG 
TGGCAGGGCG TCTACTACGC CAGACGGAAA TCCGGCGACA GCATCCAGCA GCACGTGAAG 
ATCACACCAG TGATCGGCCA AGGAGGGAAG ATCAGGCATT TCGTCTCCCT CAAGAAACTG 
TGTTGTAGCA CTGACAAAAA CAAGCAGATT CTCAAGATTC ATCGTGATTC AGGAGACAAT 
TCTCAGACAG AGTCTCATTC ATTCAGATAC AAGAACAGGA GGAAAGAGTC CATTGATGTG 
AAATCAATAT CATCTCGAGG CAGTGATGCA CCAAGTCTGC AGAACCGTCG CTACCCATCC 
ATGGCGCGGA TCCACTCCAT GACCATTGAG GCTCCCATCA CGAAGGTTAT AAATATAATC 
AACGCAGCCC AAGAAAACAG TCCTGTCACA GTGGCAGAAG CCTTGGACAG AGTTTTAGAA 
ATTTTACGAA CTACGGAACT GTACTCCCCT CAGCTCGGTA CCAAAGATGA AGATCCCCAC 
ACCAGCGACC TCGTGGGAGG GCTGATGACT GATGGCTTGA GAAGGTTGTC AGGAAACGAG 
TACGTGTTCA CTAAGAACGT ACACCAGAGT CATAGTCACC TTGCAATGCC AGGAACCATC 
ACCGACGTGC CCCCTTCCGT CGCCCAACTA CTTGACAATG AGGAAAGCTG GGAGTTCAAC 
ATCTTTGAAC TGGAAGCTGT GACGCACAAA AGGCCATTGG TTTACCTGGG CTTGAAAGTT 
TTCTCCCGGT TTGGAGTATG TGAATTCCTA CACTGTTCTG AAACTACTCT TCGGGCCTGG 
CTCCAGGTGA TCGAAGCCAA CTACCACTCT TCCAACGCTT ACCACAACTC CACCCACGCC 
GCTGACGTCC TGCACGCCAC AGCCTTCTTC CTCGGCAAGG AGAGAGTGAA GGGAAGCTTG 
GATCAGCTGG ACGAGGTGGC CGCCCTGATC GCTGCCACAG TCCACGACGT GGACCACCCG 
GGAAGGACCA ACTCTTTCCT GTGCAACGCG GGCAGTGAGC TCGCTGTGCT CTATAATGAC 
ACCGCTGTCT TGGAGAGTCA CCACACAGCC CTGGCCTTCC AGCTCACGGT CAAGGACAGC 
AAATGCAACA TTTTCAAGAA TATCGACAGG AACCATTATC GAACACTGCG CCAAGCTATT 
ATCGACATGG TTTTAGCAAC AGAGATGACA AAACACTTTG AACATGTGAA CAAATTTGTG 
AACAGCATTA ACAAGCCAAT GGCAGCTGAG ATTGACGGCA GCGACTGCGA GTGCAGCCCT 
CCTGGGAAGA ACTTCCCTGA GAACCAGACC CTGATCAAGC GCATGATGAT TAAGTGTGCG 
GATGTAGCCA ACCCCTGCCG CCCCCTGGAC CTGTGCATTG AATGGGCTGG GAGGATCTCT 
GAGGAGTACT TTGCGCAGAC GGATGAAGAG AAGAGACAGG GGCTACCCGT AGTGATGCCA 
GTGTTTGACC GGAATACCTG TAGCATCCCC AAGTCCCAAA TCTCCTTTAT TGACTACTTC 
ATAACAGACA TGTTTGATGC TTGGGATGCC TTTGCACACC TGCCAACGCT GATGCAACAT 
TTGGCTGATA ACTACAAACA CTGGAAGACA TTAGATGACC TAAAGTGCAA AAGTCTGAGG 
CTTCCATCTG ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853664.1
CDS	18..2675
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPRQTTGVSQGPAGPLHGLFVQTDAADAIPQSRASGSPGAARARRARGELGSGSSAGSAGPAATTCRGRRRHCCSSAEAETQTSYTSVKQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYSCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHAVILAVVSQASGNYEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVLSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELAELPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCSTDKNKQILKIHRDSGDNSQTESHSFRYKNRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHQSHSHLAMPGTITDVPPSVAQLLDNEESWEFNIFELEAVTHKRPLVYLGLKVFSRFGVCEFLHCSETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDSKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPMAAEIDGSDCECSPPGKNFPENQTLIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPTLMQHLADNYKHWKTLDDLKCKSLRLPSDS

Target ORF information:

RefSeq Version	XM_024997896.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997896.1

ORF Insert Sequence:

ATGGGCTGCG CCCCCAGCAT CCACGTCTCG CAGAGCGGCG TGATCTACTG CCGGGACTCG 
GACGAGTCCA ACTCGCCCCG CCAGACCACC GGCGTGTCGC AGGGCCCCGC GGGCCCCCTG 
CACGGCCTCT TCGTCCAGAC CGACGCCGCC GACGCCATCC CCCAGAGCCG CGCGTCCGGG 
TCCCCAGGCG CCGCCCGCGC CCGCAGGGCC CGCGGGGAGC TGGGCAGCGG CAGCAGCGCG 
GGCTCCGCGG GCCCCGCGGC GACCACCTGC AGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCGGAGGCCG AGACCCAGAC CAGCTACACC AGCGTCAAGC AGGTTTCTTC TGCGGAGGTA 
CGCATCGGGC CCATGAGATT GACACAGGAT CCTATCCAGG TTTTGCTCAT CTTTGCAAAG 
GAAGATAGTC AGAGTGATGG ATTCTGGTGG GCCTGTGACA GAGCCGGTTA CAGCTGCAAT 
ATTGCTCGGA CTCCAGAGTC AGCCCTGGAA TGCTTTCTAG ATAAACATCA TGAGATTATT 
GTAATCGATC ACAGACAGAC TCAAAACTTC GATGCAGAAG CAGTGTGCAG GTCAATCCGG 
GCTACGAACC CCTCGGAGCA CGCAGTGATC CTCGCCGTGG TCTCACAAGC ATCCGGCAAC 
TATGAAGAGG CCTCTGTGCT CCCTCTTCTG CACGCGGGCT TCAACAGGAG ATTTATGGAG 
AACAGCAGCA TAATCGCTTG CTACAATGAA CTGATTCAAA TAGAACATGG GGAAGTTCTC 
TCACAGTTCA AATTAAGGGC CTGTAATTCA GTGTTTACAG CATTAGACCA CTGTCACGAA 
GCCATAGAAA TCACAAGCGA TGACCACGTG ATCCAGTATG TCAACCCAGC CTTCGAGAGG 
ATGATGGGCT ACCACAAAGG CGAACTGCTG GGGAAAGAAC TCGCCGAGCT GCCCAAAAGC 
GACAAGAACC GGGCAGACCT TCTCGACACC ATCAATACCT GCATCAAGAA GGGCAAGGAG 
TGGCAGGGCG TCTACTACGC CAGACGGAAA TCCGGCGACA GCATCCAGCA GCACGTGAAG 
ATCACACCAG TGATCGGCCA AGGAGGGAAG ATCAGGCATT TCGTCTCCCT CAAGAAACTG 
TGTTGTAGCA CTGACAAAAA CAAGCAGATT CTCAAGATTC ATCGTGATTC AGGAGACAAT 
TCTCAGACAG AGTCTCATTC ATTCAGATAC AAGAACAGGA GGAAAGAGTC CATTGATGTG 
AAATCAATAT CATCTCGAGG CAGTGATGCA CCAAGTCTGC AGAACCGTCG CTACCCATCC 
ATGGCGCGGA TCCACTCCAT GACCATTGAG GCTCCCATCA CGAAGGTTAT AAATATAATC 
AACGCAGCCC AAGAAAACAG TCCTGTCACA GTGGCAGAAG CCTTGGACAG AGTTTTAGAA 
ATTTTACGAA CTACGGAACT GTACTCCCCT CAGCTCGGTA CCAAAGATGA AGATCCCCAC 
ACCAGCGACC TCGTGGGAGG GCTGATGACT GATGGCTTGA GAAGGTTGTC AGGAAACGAG 
TACGTGTTCA CTAAGAACGT ACACCAGAGT CATAGTCACC TTGCAATGCC AGGAACCATC 
ACCGACGTGC CCCCTTCCGT CGCCCAACTA CTTGACAATG AGGAAAGCTG GGAGTTCAAC 
ATCTTTGAAC TGGAAGCTGT GACGCACAAA AGGCCATTGG TTTACCTGGG CTTGAAAGTT 
TTCTCCCGGT TTGGAGTATG TGAATTCCTA CACTGTTCTG AAACTACTCT TCGGGCCTGG 
CTCCAGGTGA TCGAAGCCAA CTACCACTCT TCCAACGCTT ACCACAACTC CACCCACGCC 
GCTGACGTCC TGCACGCCAC AGCCTTCTTC CTCGGCAAGG AGAGAGTGAA GGGAAGCTTG 
GATCAGCTGG ACGAGGTGGC CGCCCTGATC GCTGCCACAG TCCACGACGT GGACCACCCG 
GGAAGGACCA ACTCTTTCCT GTGCAACGCG GGCAGTGAGC TCGCTGTGCT CTATAATGAC 
ACCGCTGTCT TGGAGAGTCA CCACACAGCC CTGGCCTTCC AGCTCACGGT CAAGGACAGC 
AAATGCAACA TTTTCAAGAA TATCGACAGG AACCATTATC GAACACTGCG CCAAGCTATT 
ATCGACATGG TTTTAGCAAC AGAGATGACA AAACACTTTG AACATGTGAA CAAATTTGTG 
AACAGCATTA ACAAGCCAAT GGCAGCTGAG ATTGACGGCA GCGACTGCGA GTGCAGCCCT 
CCTGGGAAGA ACTTCCCTGA GAACCAGACC CTGATCAAGC GCATGATGAT TAAGTGTGCG 
GATGTAGCCA ACCCCTGCCG CCCCCTGGAC CTGTGCATTG AATGGGCTGG GAGGATCTCT 
GAGGAGTACT TTGCGCAGAC GGATGAAGAG AAGAGACAGG GGCTACCCGT AGTGATGCCA 
GTGTTTGACC GGAATACCTG TAGCATCCCC AAGTCCCAAA TCTCCTTTAT TGACTACTTC 
ATAACAGACA TGTTTGATGC TTGGGATGCC TTTGCACACC TGCCAACGCT GATGCAACAT 
TTGGCTGATA ACTACAAACA CTGGAAGACA TTAGATGACC TAAAGTGCAA AAGTCTGAGG 
CTTCCATCTG ACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE8B ( XM_024997897.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853665.1 Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X2, mRNA.

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CloneID	OBa219770
Clone ID Related Accession (Same CDS sequence)	XM_024997897.1
Accession Version	XM_024997897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CCCCCAGCAT CCACGTCTCG CAGAGCGGCG TGATCTACTG CCGGGACTCG 
GACGAGTCCA ACTCGCCCCG CCAGACCACC GGCGTGTCGC AGGGCCCCGC GGGCCCCCTG 
CACGGCCTCT TCGTCCAGAC CGACGCCGCC GACGCCATCC CCCAGAGCCG CGCGTCCGGG 
TCCCCAGGCG CCGCCCGCGC CCGCAGGGCC CGCGGGGAGC TGGGCAGCGG CAGCAGCGCG 
GGCTCCGCGG GCCCCGCGGC GACCACCTGC AGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCGGAGGCCG AGACCCAGAC CAGCTACACC AGCGTCAAGG TTTCTTCTGC GGAGGTACGC 
ATCGGGCCCA TGAGATTGAC ACAGGATCCT ATCCAGGTTT TGCTCATCTT TGCAAAGGAA 
GATAGTCAGA GTGATGGATT CTGGTGGGCC TGTGACAGAG CCGGTTACAG CTGCAATATT 
GCTCGGACTC CAGAGTCAGC CCTGGAATGC TTTCTAGATA AACATCATGA GATTATTGTA 
ATCGATCACA GACAGACTCA AAACTTCGAT GCAGAAGCAG TGTGCAGGTC AATCCGGGCT 
ACGAACCCCT CGGAGCACGC AGTGATCCTC GCCGTGGTCT CACAAGCATC CGGCAACTAT 
GAAGAGGCCT CTGTGCTCCC TCTTCTGCAC GCGGGCTTCA ACAGGAGATT TATGGAGAAC 
AGCAGCATAA TCGCTTGCTA CAATGAACTG ATTCAAATAG AACATGGGGA AGTTCTCTCA 
CAGTTCAAAT TAAGGGCCTG TAATTCAGTG TTTACAGCAT TAGACCACTG TCACGAAGCC 
ATAGAAATCA CAAGCGATGA CCACGTGATC CAGTATGTCA ACCCAGCCTT CGAGAGGATG 
ATGGGCTACC ACAAAGGCGA ACTGCTGGGG AAAGAACTCG CCGAGCTGCC CAAAAGCGAC 
AAGAACCGGG CAGACCTTCT CGACACCATC AATACCTGCA TCAAGAAGGG CAAGGAGTGG 
CAGGGCGTCT ACTACGCCAG ACGGAAATCC GGCGACAGCA TCCAGCAGCA CGTGAAGATC 
ACACCAGTGA TCGGCCAAGG AGGGAAGATC AGGCATTTCG TCTCCCTCAA GAAACTGTGT 
TGTAGCACTG ACAAAAACAA GCAGATTCTC AAGATTCATC GTGATTCAGG AGACAATTCT 
CAGACAGAGT CTCATTCATT CAGATACAAG AACAGGAGGA AAGAGTCCAT TGATGTGAAA 
TCAATATCAT CTCGAGGCAG TGATGCACCA AGTCTGCAGA ACCGTCGCTA CCCATCCATG 
GCGCGGATCC ACTCCATGAC CATTGAGGCT CCCATCACGA AGGTTATAAA TATAATCAAC 
GCAGCCCAAG AAAACAGTCC TGTCACAGTG GCAGAAGCCT TGGACAGAGT TTTAGAAATT 
TTACGAACTA CGGAACTGTA CTCCCCTCAG CTCGGTACCA AAGATGAAGA TCCCCACACC 
AGCGACCTCG TGGGAGGGCT GATGACTGAT GGCTTGAGAA GGTTGTCAGG AAACGAGTAC 
GTGTTCACTA AGAACGTACA CCAGAGTCAT AGTCACCTTG CAATGCCAGG AACCATCACC 
GACGTGCCCC CTTCCGTCGC CCAACTACTT GACAATGAGG AAAGCTGGGA GTTCAACATC 
TTTGAACTGG AAGCTGTGAC GCACAAAAGG CCATTGGTTT ACCTGGGCTT GAAAGTTTTC 
TCCCGGTTTG GAGTATGTGA ATTCCTACAC TGTTCTGAAA CTACTCTTCG GGCCTGGCTC 
CAGGTGATCG AAGCCAACTA CCACTCTTCC AACGCTTACC ACAACTCCAC CCACGCCGCT 
GACGTCCTGC ACGCCACAGC CTTCTTCCTC GGCAAGGAGA GAGTGAAGGG AAGCTTGGAT 
CAGCTGGACG AGGTGGCCGC CCTGATCGCT GCCACAGTCC ACGACGTGGA CCACCCGGGA 
AGGACCAACT CTTTCCTGTG CAACGCGGGC AGTGAGCTCG CTGTGCTCTA TAATGACACC 
GCTGTCTTGG AGAGTCACCA CACAGCCCTG GCCTTCCAGC TCACGGTCAA GGACAGCAAA 
TGCAACATTT TCAAGAATAT CGACAGGAAC CATTATCGAA CACTGCGCCA AGCTATTATC 
GACATGGTTT TAGCAACAGA GATGACAAAA CACTTTGAAC ATGTGAACAA ATTTGTGAAC 
AGCATTAACA AGCCAATGGC AGCTGAGATT GACGGCAGCG ACTGCGAGTG CAGCCCTCCT 
GGGAAGAACT TCCCTGAGAA CCAGACCCTG ATCAAGCGCA TGATGATTAA GTGTGCGGAT 
GTAGCCAACC CCTGCCGCCC CCTGGACCTG TGCATTGAAT GGGCTGGGAG GATCTCTGAG 
GAGTACTTTG CGCAGACGGA TGAAGAGAAG AGACAGGGGC TACCCGTAGT GATGCCAGTG 
TTTGACCGGA ATACCTGTAG CATCCCCAAG TCCCAAATCT CCTTTATTGA CTACTTCATA 
ACAGACATGT TTGATGCTTG GGATGCCTTT GCACACCTGC CAACGCTGAT GCAACATTTG 
GCTGATAACT ACAAACACTG GAAGACATTA GATGACCTAA AGTGCAAAAG TCTGAGGCTT 
CCATCTGACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853665.1
CDS	18..2672
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPRQTTGVSQGPAGPLHGLFVQTDAADAIPQSRASGSPGAARARRARGELGSGSSAGSAGPAATTCRGRRRHCCSSAEAETQTSYTSVKVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYSCNIARTPESALECFLDKHHEIIVIDHRQTQNFDAEAVCRSIRATNPSEHAVILAVVSQASGNYEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVLSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELAELPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCSTDKNKQILKIHRDSGDNSQTESHSFRYKNRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHQSHSHLAMPGTITDVPPSVAQLLDNEESWEFNIFELEAVTHKRPLVYLGLKVFSRFGVCEFLHCSETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDSKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPMAAEIDGSDCECSPPGKNFPENQTLIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPTLMQHLADNYKHWKTLDDLKCKSLRLPSDS

Target ORF information:

RefSeq Version	XM_024997897.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 8B (PDE8B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997897.1

ORF Insert Sequence:

ATGGGCTGCG CCCCCAGCAT CCACGTCTCG CAGAGCGGCG TGATCTACTG CCGGGACTCG 
GACGAGTCCA ACTCGCCCCG CCAGACCACC GGCGTGTCGC AGGGCCCCGC GGGCCCCCTG 
CACGGCCTCT TCGTCCAGAC CGACGCCGCC GACGCCATCC CCCAGAGCCG CGCGTCCGGG 
TCCCCAGGCG CCGCCCGCGC CCGCAGGGCC CGCGGGGAGC TGGGCAGCGG CAGCAGCGCG 
GGCTCCGCGG GCCCCGCGGC GACCACCTGC AGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCGGAGGCCG AGACCCAGAC CAGCTACACC AGCGTCAAGG TTTCTTCTGC GGAGGTACGC 
ATCGGGCCCA TGAGATTGAC ACAGGATCCT ATCCAGGTTT TGCTCATCTT TGCAAAGGAA 
GATAGTCAGA GTGATGGATT CTGGTGGGCC TGTGACAGAG CCGGTTACAG CTGCAATATT 
GCTCGGACTC CAGAGTCAGC CCTGGAATGC TTTCTAGATA AACATCATGA GATTATTGTA 
ATCGATCACA GACAGACTCA AAACTTCGAT GCAGAAGCAG TGTGCAGGTC AATCCGGGCT 
ACGAACCCCT CGGAGCACGC AGTGATCCTC GCCGTGGTCT CACAAGCATC CGGCAACTAT 
GAAGAGGCCT CTGTGCTCCC TCTTCTGCAC GCGGGCTTCA ACAGGAGATT TATGGAGAAC 
AGCAGCATAA TCGCTTGCTA CAATGAACTG ATTCAAATAG AACATGGGGA AGTTCTCTCA 
CAGTTCAAAT TAAGGGCCTG TAATTCAGTG TTTACAGCAT TAGACCACTG TCACGAAGCC 
ATAGAAATCA CAAGCGATGA CCACGTGATC CAGTATGTCA ACCCAGCCTT CGAGAGGATG 
ATGGGCTACC ACAAAGGCGA ACTGCTGGGG AAAGAACTCG CCGAGCTGCC CAAAAGCGAC 
AAGAACCGGG CAGACCTTCT CGACACCATC AATACCTGCA TCAAGAAGGG CAAGGAGTGG 
CAGGGCGTCT ACTACGCCAG ACGGAAATCC GGCGACAGCA TCCAGCAGCA CGTGAAGATC 
ACACCAGTGA TCGGCCAAGG AGGGAAGATC AGGCATTTCG TCTCCCTCAA GAAACTGTGT 
TGTAGCACTG ACAAAAACAA GCAGATTCTC AAGATTCATC GTGATTCAGG AGACAATTCT 
CAGACAGAGT CTCATTCATT CAGATACAAG AACAGGAGGA AAGAGTCCAT TGATGTGAAA 
TCAATATCAT CTCGAGGCAG TGATGCACCA AGTCTGCAGA ACCGTCGCTA CCCATCCATG 
GCGCGGATCC ACTCCATGAC CATTGAGGCT CCCATCACGA AGGTTATAAA TATAATCAAC 
GCAGCCCAAG AAAACAGTCC TGTCACAGTG GCAGAAGCCT TGGACAGAGT TTTAGAAATT 
TTACGAACTA CGGAACTGTA CTCCCCTCAG CTCGGTACCA AAGATGAAGA TCCCCACACC 
AGCGACCTCG TGGGAGGGCT GATGACTGAT GGCTTGAGAA GGTTGTCAGG AAACGAGTAC 
GTGTTCACTA AGAACGTACA CCAGAGTCAT AGTCACCTTG CAATGCCAGG AACCATCACC 
GACGTGCCCC CTTCCGTCGC CCAACTACTT GACAATGAGG AAAGCTGGGA GTTCAACATC 
TTTGAACTGG AAGCTGTGAC GCACAAAAGG CCATTGGTTT ACCTGGGCTT GAAAGTTTTC 
TCCCGGTTTG GAGTATGTGA ATTCCTACAC TGTTCTGAAA CTACTCTTCG GGCCTGGCTC 
CAGGTGATCG AAGCCAACTA CCACTCTTCC AACGCTTACC ACAACTCCAC CCACGCCGCT 
GACGTCCTGC ACGCCACAGC CTTCTTCCTC GGCAAGGAGA GAGTGAAGGG AAGCTTGGAT 
CAGCTGGACG AGGTGGCCGC CCTGATCGCT GCCACAGTCC ACGACGTGGA CCACCCGGGA 
AGGACCAACT CTTTCCTGTG CAACGCGGGC AGTGAGCTCG CTGTGCTCTA TAATGACACC 
GCTGTCTTGG AGAGTCACCA CACAGCCCTG GCCTTCCAGC TCACGGTCAA GGACAGCAAA 
TGCAACATTT TCAAGAATAT CGACAGGAAC CATTATCGAA CACTGCGCCA AGCTATTATC 
GACATGGTTT TAGCAACAGA GATGACAAAA CACTTTGAAC ATGTGAACAA ATTTGTGAAC 
AGCATTAACA AGCCAATGGC AGCTGAGATT GACGGCAGCG ACTGCGAGTG CAGCCCTCCT 
GGGAAGAACT TCCCTGAGAA CCAGACCCTG ATCAAGCGCA TGATGATTAA GTGTGCGGAT 
GTAGCCAACC CCTGCCGCCC CCTGGACCTG TGCATTGAAT GGGCTGGGAG GATCTCTGAG 
GAGTACTTTG CGCAGACGGA TGAAGAGAAG AGACAGGGGC TACCCGTAGT GATGCCAGTG 
TTTGACCGGA ATACCTGTAG CATCCCCAAG TCCCAAATCT CCTTTATTGA CTACTTCATA 
ACAGACATGT TTGATGCTTG GGATGCCTTT GCACACCTGC CAACGCTGAT GCAACATTTG 
GCTGATAACT ACAAACACTG GAAGACATTA GATGACCTAA AGTGCAAAAG TCTGAGGCTT 
CCATCTGACA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PDE8B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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