WNK4 cDNA ORF clone, Bos taurus(cattle)

The following WNK4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa226908 XM_024980893.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa226904 XM_024980888.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226905 XM_024980889.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226906 XM_024980891.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa226907 XM_024980892.1
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa30079 XM_005220804.4
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30079 XM_005195752.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30080 XM_005220805.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30080 XM_005195753.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30081 XM_003583605.4
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30081 XM_003587441.5
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30082 XM_005220806.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30082 XM_005195754.3
Latest version!
Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OBa30081 XM_003587441.4 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa30079 XM_005220804.3 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa226908
    Clone ID Related Accession (Same CDS sequence) XM_024980893.1
    Accession Version XM_024980893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGACCCAC
    ACTTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836661.1
    CDS122..3070
    Translation

    Target ORF information:

    RefSeq Version XM_024980893.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGACCCAC
    ACTTTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226904
    Clone ID Related Accession (Same CDS sequence) XM_024980888.1
    Accession Version XM_024980888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGTGAGA CAGAAGTCAC CAGCTCCCAT TGTTCCAGAG TCCCCTCCCT AGTGATCTGT
    CTTTCAGGCC CTGGGAATCT GCCCCTTGCT GTGGGGGTAC TAGCCCCCCA CTTTTCCCTG
    TGGCCCCTTC CCTCATTCAG TGCTCTCCCT CCCCATCCTG CACCCAGGCA TCATGCGAAG
    GAACTCCCTG AGTGGCAGCA GCACCGGCTC CCAGGAGCAG CGGGCAAGCA AGGGGGTGAC
    ATTCGCCGGG GATGTTGGCA GGATGTGAAT TATGAACAGA AGCCATGTGT CTCACCCACA
    CCTGGGCCCA CCATGGAGCT TATGCCCTTA GAATCTGCTG ACCAACACAA CTCTGCGAGG
    AAGACCTCAG CACTGAGGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836656.1
    CDS120..4103
    Translation

    Target ORF information:

    RefSeq Version XM_024980888.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGTGAGA CAGAAGTCAC CAGCTCCCAT TGTTCCAGAG TCCCCTCCCT AGTGATCTGT
    CTTTCAGGCC CTGGGAATCT GCCCCTTGCT GTGGGGGTAC TAGCCCCCCA CTTTTCCCTG
    TGGCCCCTTC CCTCATTCAG TGCTCTCCCT CCCCATCCTG CACCCAGGCA TCATGCGAAG
    GAACTCCCTG AGTGGCAGCA GCACCGGCTC CCAGGAGCAG CGGGCAAGCA AGGGGGTGAC
    ATTCGCCGGG GATGTTGGCA GGATGTGAAT TATGAACAGA AGCCATGTGT CTCACCCACA
    CCTGGGCCCA CCATGGAGCT TATGCCCTTA GAATCTGCTG ACCAACACAA CTCTGCGAGG
    AAGACCTCAG CACTGAGGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226905
    Clone ID Related Accession (Same CDS sequence) XM_024980889.1
    Accession Version XM_024980889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC
    ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT
    CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC
    TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT
    CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG
    AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC
    TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC
    TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC
    CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT
    CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA
    AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG
    CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC
    CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC
    TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG
    GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT
    GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG
    ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG
    GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG
    GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG
    CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC
    AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG
    GGTGAGACAG AAGTCACCAG CTCCCATTGT TCCAGAGTCC CCTCCCTAGT GATCTGTCTT
    TCAGGCCCTG GGAATCTGCC CCTTGCTGTG GGGGTACTAG CCCCCCACTT TTCCCTGTGG
    CCCCTTCCCT CATTCAGTGC TCTCCCTCCC CATCCTGCAC CCAGGCATCA TGCGAAGGAA
    CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG GCAAGCAAGG GGGTGACATT
    CGCCGGGGAT GTTGGCAGGA TGTGAATTAT GAACAGAAGC CATGTGTCTC ACCCACACCT
    GGGCCCACCA TGGAGCTTAT GCCCTTAGAA TCTGCTGACC AACACAACTC TGCGAGGAAG
    ACCTCAGCAC TGAGGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836657.1
    CDS120..4100
    Translation

    Target ORF information:

    RefSeq Version XM_024980889.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC
    ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT
    CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC
    TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT
    CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG
    AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC
    TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC
    TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC
    CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT
    CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA
    AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG
    CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC
    CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC
    TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG
    GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT
    GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG
    ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG
    GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG
    GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG
    CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC
    AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG
    GGTGAGACAG AAGTCACCAG CTCCCATTGT TCCAGAGTCC CCTCCCTAGT GATCTGTCTT
    TCAGGCCCTG GGAATCTGCC CCTTGCTGTG GGGGTACTAG CCCCCCACTT TTCCCTGTGG
    CCCCTTCCCT CATTCAGTGC TCTCCCTCCC CATCCTGCAC CCAGGCATCA TGCGAAGGAA
    CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG GCAAGCAAGG GGGTGACATT
    CGCCGGGGAT GTTGGCAGGA TGTGAATTAT GAACAGAAGC CATGTGTCTC ACCCACACCT
    GGGCCCACCA TGGAGCTTAT GCCCTTAGAA TCTGCTGACC AACACAACTC TGCGAGGAAG
    ACCTCAGCAC TGAGGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226906
    Clone ID Related Accession (Same CDS sequence) XM_024980891.1
    Accession Version XM_024980891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC
    ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT
    CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC
    TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT
    CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG
    AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC
    TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC
    TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC
    CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT
    CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA
    AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG
    CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC
    CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC
    TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG
    GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT
    GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG
    ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG
    GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG
    GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG
    CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC
    AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG
    GGCATCATGC GAAGGAACTC CCTGAGTGGC AGCAGCACCG GCTCCCAGGA GCAGCGGGCA
    AGCAAGGGGG TGACATTCGC CGGGGATGTT GGCAGGATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836659.1
    CDS120..3821
    Translation

    Target ORF information:

    RefSeq Version XM_024980891.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC
    ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT
    CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC
    TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT
    CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG
    AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC
    TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC
    TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC
    CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT
    CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA
    AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG
    CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC
    CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC
    TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG
    GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT
    GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG
    ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG
    GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG
    GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG
    CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC
    AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG
    GGCATCATGC GAAGGAACTC CCTGAGTGGC AGCAGCACCG GCTCCCAGGA GCAGCGGGCA
    AGCAAGGGGG TGACATTCGC CGGGGATGTT GGCAGGATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226907
    Clone ID Related Accession (Same CDS sequence) XM_024980892.1
    Accession Version XM_024980892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAACCAC GAGTCCTTCA GCGCTGGAGC CGCCAGATCC TGCGGGGGCT CCATTTCCTA 
    CACTCCCGAG TACCCCCTAT CCTGCACCGA GACCTCAAGT GTGACAACGT CTTTATCACC
    GGCCCTTCGG GCTCCGTTAA GATCGGGGAC CTGGGCCTGG CCACGCTCAA GCGCGCCTCC
    TTCGCCAAGA GCGTCATCGG GACCCCGGAG TTCATGGCCC CAGAGATGTA CGAGGAAAAG
    TACGATGAGG CCGTGGACGT GTACGCGTTT GGCATGTGCA TGCTGGAGAT GGCGACCTCG
    GAGTACCCCT ACTCAGAGTG CCAGAATGCC GCTCAAATCT ACCGCAAGGT CACCTCGGGC
    ACGAAACCCA ACAGCTTCTA CAAGGTGAAG ATGCCCGAGG TAAAGGAGAT CATTGAAGGC
    TGCATCCGCA CGGATAAGAA TGAGAGGTTC ACCATCCACG ACCTTCTGGC TCACGCCTTC
    TTCCGCGAGG AGCGCGGCGT GCACGTGGAG TTGGCGGAGG AGGACGACGG AGAGAAGCCG
    GACCTCAAGC TCTGGCTGCG CATGGAGGAC GCGCGGCGAG GGGGGCGCCC ACGGGACAAC
    CAGGCCATCG AGTTCTTGTT CCAGTTGGGC CGGGACGCGG CAGAGGAGGT GGCTCAGGAG
    ATGGTGGCCC TGGGCTTAGT GTGTGAAGCT GATTACCAGC CAGTGGCTCG TGCAGTGCGT
    GAACGGGTTG CTGCCATCCA GCGAAAGCGT GAGAAGCTGC GTAAAGCTAG GGAGTTGGAG
    GCCCTCCCCC CAGCGCCGAG ACCCCCACCA GCAGCTGTCC CCATGACTCC TGGTCCCTCC
    AGTGCCTTCC CTCCTGAGCC CGAGGAGCCA GAGGCAGACC AGCACCAGCC CTTCCTCTTC
    CGCCATGCCA GCTATTCATC TACCACCTCG GATTGCGAGA CTGATGGCTA CCTCAGCTCT
    TCCGGCTTCC TGGATGCCTC AGACCCTGCC CTTCAGCCCC CTAGTGGGGT GCCATCCAGC
    CCCGCTGAGT CCCATCTCCG CCTGCCCGCG GCTTTTGCTC TATCCATTCC ACGTTCTGGC
    CCCGGCAATG ACTTTTCCCC TGGAGAGAGT TATGCCTCAG AGGCAGCATC AGGCCTTAGT
    GATGTGGGAG AAGGGATGGA ACGGATGAGG AGACCCCCAG GGAAAAACCT CCGGCGTAGA
    CCTCGATCCC GGCTTCGGGT CACAAGTGTC TCAGACCAGA ATGACAGAGT GGTTGAATGC
    CAGCTGCAGA CGCACAACAG CAAGATGGTG ACCTTCCGAT TTGATCTGGA TGGGGACAGC
    CCAGAAGAGA TCGCAGCTGC CATGGTGTAC AATGAGTTCA TTCTACCCTC GGAGCGAGCT
    GGATTCCTGA ACCGGATTCG GGAGATTATC CAGCGAGTGG AGACCCTGTT GAAGAGAGAT
    ACTGGCCCTG TGGAGGCTGC TGAAGACCCT CTGAGTCCCC AGGAGGAGTC AGCACCACTG
    CCTGCTCTCC CGGGGTCCCC CCCAGACCCA TCCAGGGCAG AGCTCCAGAG CAGCACCTCC
    CTGGAGCAAA GATGCTGGGC AGCTTTTTCC ACCTCCACAT CTTCTCCTGG AACCCCCTTG
    TCTCCTGGAA GCTCCTTTTC TCCTGGAACT CCTGTTTTCC CAAGTCCCAT CCTTCCCATC
    ACTTCTCCCC CATGTCATCT CAACCCCTCC TCATTCTCCC CAATTTCTCC TCAGGCCTCC
    TCAAATCTCT CTCCACACCC CCAAAGCTGT CCACTTCCAC TCTCCCCCAG GGCACCCCAG
    TTTCCAGTCC CATCTGCTCA GTTTCCACAG AGTTCTCTCT TCCCCGATTG TTTCCAGGGC
    ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC TCTGCTTGTC CCCTCCCCTC CACCACCGCA
    GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC TCTCTGGCTG TGATGACCGT GGCTCAGTCC
    CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC
    CCTAGCCTGC CCCCTCCCAC TCCCTGTACT CCTTGTGGCC AGGAGTGGCC TTCATCTCTG
    ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA AATCTTGGTC AGCCACTCCC GGGTGAAGCC
    AGATTGGCGC CCATCTCGAA AGAGGGAAAG CCTCAGCTTG TTGGGCGCTT CCAAGTGACT
    TCATCCAAGG AGCCAGCTGA GCCTCTTCCC CTGCAGCTGG CATCCCCAAC TCCCTCTGGC
    TCCCTGAAGC CTCCAACCCC TCAGCTGACC TCGGAGAGCT CGGACACAGA GGACAGTGCT
    GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA
    GGGGCTGGAG CTGAAGAGGA AGGGGACGAT GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT
    CCACCCCTGA GCCATCCTAG CCCAGTGTGG ATGAGCTACT CCTATAGCAG CCTGTGTCTG
    AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG GACGAGGAAT TCTGGGCTGA GCTGCAGAAT
    CTTCGGCAGA AGCACTTGTC AGAGGTGGAG GCACTACAGA CACTACAGAA ACAGGAAATC
    GAGGACTTGT ATAGCAGGCT TGGAAAGCAG CCCCCACCAG GGATCGTGGC CCCTGCTGCT
    ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC ACGGCGCAAC
    AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG GGTGAGACAG AAGTCACCAG CTCCCATTGT
    TCCAGAGTCC CCTCCCTAGT GATCTGTCTT TCAGGCCCTG GGAATCTGCC CCTTGCTGTG
    GGGGTACTAG CCCCCCACTT TTCCCTGTGG CCCCTTCCCT CATTCAGTGC TCTCCCTCCC
    CATCCTGCAC CCAGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA
    GGAGCAGCGG GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGAATTAT
    GAACAGAAGC CATGTGTCTC ACCCACACCT GGGCCCACCA TGGAGCTTAT GCCCTTAGAA
    TCTGCTGACC AACACAACTC TGCGAGGAAG ACCTCAGCAC TGAGGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836660.1
    CDS136..3306
    Translation

    Target ORF information:

    RefSeq Version XM_024980892.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAACCAC GAGTCCTTCA GCGCTGGAGC CGCCAGATCC TGCGGGGGCT CCATTTCCTA 
    CACTCCCGAG TACCCCCTAT CCTGCACCGA GACCTCAAGT GTGACAACGT CTTTATCACC
    GGCCCTTCGG GCTCCGTTAA GATCGGGGAC CTGGGCCTGG CCACGCTCAA GCGCGCCTCC
    TTCGCCAAGA GCGTCATCGG GACCCCGGAG TTCATGGCCC CAGAGATGTA CGAGGAAAAG
    TACGATGAGG CCGTGGACGT GTACGCGTTT GGCATGTGCA TGCTGGAGAT GGCGACCTCG
    GAGTACCCCT ACTCAGAGTG CCAGAATGCC GCTCAAATCT ACCGCAAGGT CACCTCGGGC
    ACGAAACCCA ACAGCTTCTA CAAGGTGAAG ATGCCCGAGG TAAAGGAGAT CATTGAAGGC
    TGCATCCGCA CGGATAAGAA TGAGAGGTTC ACCATCCACG ACCTTCTGGC TCACGCCTTC
    TTCCGCGAGG AGCGCGGCGT GCACGTGGAG TTGGCGGAGG AGGACGACGG AGAGAAGCCG
    GACCTCAAGC TCTGGCTGCG CATGGAGGAC GCGCGGCGAG GGGGGCGCCC ACGGGACAAC
    CAGGCCATCG AGTTCTTGTT CCAGTTGGGC CGGGACGCGG CAGAGGAGGT GGCTCAGGAG
    ATGGTGGCCC TGGGCTTAGT GTGTGAAGCT GATTACCAGC CAGTGGCTCG TGCAGTGCGT
    GAACGGGTTG CTGCCATCCA GCGAAAGCGT GAGAAGCTGC GTAAAGCTAG GGAGTTGGAG
    GCCCTCCCCC CAGCGCCGAG ACCCCCACCA GCAGCTGTCC CCATGACTCC TGGTCCCTCC
    AGTGCCTTCC CTCCTGAGCC CGAGGAGCCA GAGGCAGACC AGCACCAGCC CTTCCTCTTC
    CGCCATGCCA GCTATTCATC TACCACCTCG GATTGCGAGA CTGATGGCTA CCTCAGCTCT
    TCCGGCTTCC TGGATGCCTC AGACCCTGCC CTTCAGCCCC CTAGTGGGGT GCCATCCAGC
    CCCGCTGAGT CCCATCTCCG CCTGCCCGCG GCTTTTGCTC TATCCATTCC ACGTTCTGGC
    CCCGGCAATG ACTTTTCCCC TGGAGAGAGT TATGCCTCAG AGGCAGCATC AGGCCTTAGT
    GATGTGGGAG AAGGGATGGA ACGGATGAGG AGACCCCCAG GGAAAAACCT CCGGCGTAGA
    CCTCGATCCC GGCTTCGGGT CACAAGTGTC TCAGACCAGA ATGACAGAGT GGTTGAATGC
    CAGCTGCAGA CGCACAACAG CAAGATGGTG ACCTTCCGAT TTGATCTGGA TGGGGACAGC
    CCAGAAGAGA TCGCAGCTGC CATGGTGTAC AATGAGTTCA TTCTACCCTC GGAGCGAGCT
    GGATTCCTGA ACCGGATTCG GGAGATTATC CAGCGAGTGG AGACCCTGTT GAAGAGAGAT
    ACTGGCCCTG TGGAGGCTGC TGAAGACCCT CTGAGTCCCC AGGAGGAGTC AGCACCACTG
    CCTGCTCTCC CGGGGTCCCC CCCAGACCCA TCCAGGGCAG AGCTCCAGAG CAGCACCTCC
    CTGGAGCAAA GATGCTGGGC AGCTTTTTCC ACCTCCACAT CTTCTCCTGG AACCCCCTTG
    TCTCCTGGAA GCTCCTTTTC TCCTGGAACT CCTGTTTTCC CAAGTCCCAT CCTTCCCATC
    ACTTCTCCCC CATGTCATCT CAACCCCTCC TCATTCTCCC CAATTTCTCC TCAGGCCTCC
    TCAAATCTCT CTCCACACCC CCAAAGCTGT CCACTTCCAC TCTCCCCCAG GGCACCCCAG
    TTTCCAGTCC CATCTGCTCA GTTTCCACAG AGTTCTCTCT TCCCCGATTG TTTCCAGGGC
    ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC TCTGCTTGTC CCCTCCCCTC CACCACCGCA
    GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC TCTCTGGCTG TGATGACCGT GGCTCAGTCC
    CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC
    CCTAGCCTGC CCCCTCCCAC TCCCTGTACT CCTTGTGGCC AGGAGTGGCC TTCATCTCTG
    ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA AATCTTGGTC AGCCACTCCC GGGTGAAGCC
    AGATTGGCGC CCATCTCGAA AGAGGGAAAG CCTCAGCTTG TTGGGCGCTT CCAAGTGACT
    TCATCCAAGG AGCCAGCTGA GCCTCTTCCC CTGCAGCTGG CATCCCCAAC TCCCTCTGGC
    TCCCTGAAGC CTCCAACCCC TCAGCTGACC TCGGAGAGCT CGGACACAGA GGACAGTGCT
    GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA
    GGGGCTGGAG CTGAAGAGGA AGGGGACGAT GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT
    CCACCCCTGA GCCATCCTAG CCCAGTGTGG ATGAGCTACT CCTATAGCAG CCTGTGTCTG
    AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG GACGAGGAAT TCTGGGCTGA GCTGCAGAAT
    CTTCGGCAGA AGCACTTGTC AGAGGTGGAG GCACTACAGA CACTACAGAA ACAGGAAATC
    GAGGACTTGT ATAGCAGGCT TGGAAAGCAG CCCCCACCAG GGATCGTGGC CCCTGCTGCT
    ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC ACGGCGCAAC
    AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG GGTGAGACAG AAGTCACCAG CTCCCATTGT
    TCCAGAGTCC CCTCCCTAGT GATCTGTCTT TCAGGCCCTG GGAATCTGCC CCTTGCTGTG
    GGGGTACTAG CCCCCCACTT TTCCCTGTGG CCCCTTCCCT CATTCAGTGC TCTCCCTCCC
    CATCCTGCAC CCAGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA
    GGAGCAGCGG GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGAATTAT
    GAACAGAAGC CATGTGTCTC ACCCACACCT GGGCCCACCA TGGAGCTTAT GCCCTTAGAA
    TCTGCTGACC AACACAACTC TGCGAGGAAG ACCTCAGCAC TGAGGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30079
    Clone ID Related Accession (Same CDS sequence) XM_005195752.3 , XM_005220804.3 , XM_005220804.4
    Accession Version XM_005195752.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT
    ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195809.2
    CDS113..3835
    Translation

    Target ORF information:

    RefSeq Version XM_005195752.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195752.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT
    ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30079
    Clone ID Related Accession (Same CDS sequence) XM_005195752.3 , XM_005220804.3 , XM_005220804.4
    Accession Version XM_005220804.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220804.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT
    ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220861.1
    CDS113..3835
    Translation

    Target ORF information:

    RefSeq Version XM_005220804.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220804.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT
    ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30079
    Clone ID Related Accession (Same CDS sequence) XM_005195752.3 , XM_005220804.3 , XM_005220804.4
    Accession Version XM_005220804.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220804.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT
    ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220861.1
    CDS120..3842
    Translation

    Target ORF information:

    RefSeq Version XM_005220804.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220804.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT
    ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30080
    Clone ID Related Accession (Same CDS sequence) XM_005220805.3 , XM_005195753.3
    Accession Version XM_005220805.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220805.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC
    ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT
    CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC
    TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT
    CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG
    AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC
    TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC
    TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC
    CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT
    CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA
    AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG
    CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC
    CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC
    TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG
    GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT
    GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG
    ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG
    GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG
    GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG
    CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC
    AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG
    GTGAATTATG AACAGAAGCC ATGTGTCTCA CCCACACCTG GGCCCACCAT GGAGCTTATG
    CCCTTAGAAT CTGCTGACCA ACACAACTCT GCGAGGAAGA CCTCAGCACT GAGGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220862.1
    CDS113..3832
    Translation

    Target ORF information:

    RefSeq Version XM_005220805.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220805.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC
    ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT
    CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC
    TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT
    CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG
    AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC
    TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC
    TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC
    CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT
    CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA
    AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG
    CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC
    CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC
    TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG
    GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT
    GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG
    ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG
    GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG
    GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG
    CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC
    AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG
    GTGAATTATG AACAGAAGCC ATGTGTCTCA CCCACACCTG GGCCCACCAT GGAGCTTATG
    CCCTTAGAAT CTGCTGACCA ACACAACTCT GCGAGGAAGA CCTCAGCACT GAGGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30080
    Clone ID Related Accession (Same CDS sequence) XM_005220805.3 , XM_005195753.3
    Accession Version XM_005195753.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC
    ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT
    CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC
    TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT
    CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG
    AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC
    TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC
    TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC
    CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT
    CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA
    AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG
    CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC
    CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC
    TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG
    GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT
    GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG
    ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG
    GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG
    GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG
    CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC
    AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG
    GTGAATTATG AACAGAAGCC ATGTGTCTCA CCCACACCTG GGCCCACCAT GGAGCTTATG
    CCCTTAGAAT CTGCTGACCA ACACAACTCT GCGAGGAAGA CCTCAGCACT GAGGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195810.2
    CDS113..3832
    Translation

    Target ORF information:

    RefSeq Version XM_005195753.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195753.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC
    ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT
    CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC
    TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT
    CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG
    AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC
    TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC
    TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC
    CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT
    CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA
    AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG
    CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC
    CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC
    TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG
    GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT
    GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG
    ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG
    GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG
    GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG
    CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC
    AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG
    GTGAATTATG AACAGAAGCC ATGTGTCTCA CCCACACCTG GGCCCACCAT GGAGCTTATG
    CCCTTAGAAT CTGCTGACCA ACACAACTCT GCGAGGAAGA CCTCAGCACT GAGGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30081
    Clone ID Related Accession (Same CDS sequence) XM_003587441.5 , XM_003583605.4 , XM_003587441.4
    Accession Version XM_003587441.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587441.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587489.1
    CDS120..3824
    Translation

    Target ORF information:

    RefSeq Version XM_003587441.5
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587441.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30081
    Clone ID Related Accession (Same CDS sequence) XM_003587441.5 , XM_003583605.4 , XM_003587441.4
    Accession Version XM_003583605.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583605.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003583653.2
    CDS1..3705
    Translation

    Target ORF information:

    RefSeq Version XM_003583605.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003583605.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30081
    Clone ID Related Accession (Same CDS sequence) XM_003587441.5 , XM_003583605.4 , XM_003587441.4
    Accession Version XM_003587441.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587441.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003587489.1
    CDS1..3705
    Translation

    Target ORF information:

    RefSeq Version XM_003587441.4
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003587441.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30082
    Clone ID Related Accession (Same CDS sequence) XM_005195754.3 , XM_005220806.3
    Accession Version XM_005195754.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195811.2
    CDS113..3775
    Translation

    Target ORF information:

    RefSeq Version XM_005195754.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195754.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30082
    Clone ID Related Accession (Same CDS sequence) XM_005195754.3 , XM_005220806.3
    Accession Version XM_005220806.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product serine/threonine-protein kinase WNK4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220806.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220863.1
    CDS113..3775
    Translation

    Target ORF information:

    RefSeq Version XM_005220806.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220806.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
    CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC
    CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC
    CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT
    CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC
    AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC
    TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG
    ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG
    GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT
    GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG
    GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG
    GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG
    AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC
    ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG
    AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC
    CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG
    GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG
    GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG
    TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT
    GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG
    AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG
    TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG
    GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG
    GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG
    GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA
    GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG
    CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA
    CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG
    CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC
    TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT
    GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC
    GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG
    AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG
    AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT
    GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG
    GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG
    TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT
    ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC
    CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC
    CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT
    TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA
    ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC
    TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC
    TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA
    CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC
    CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC
    TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG
    TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT
    ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT
    CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA
    AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT
    CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG
    ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT
    CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC
    GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG
    TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA
    GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG
    GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG
    CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC
    TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC
    CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.