WNK4 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024980892.1	XP_024836660.1	serine/threonine-protein kinase WNK4 isoform X6
XM_005220805.3	XP_005220862.1	serine/threonine-protein kinase WNK4 isoform X2
XM_005195752.3	XP_005195809.2	serine/threonine-protein kinase WNK4 isoform X1
XM_024980888.1	XP_024836656.1	serine/threonine-protein kinase WNK4 isoform X1
XM_003587441.5	XP_003587489.1	serine/threonine-protein kinase WNK4 isoform X4
XM_005195754.3	XP_005195811.2	serine/threonine-protein kinase WNK4 isoform X4
XM_005220804.3	XP_005220861.1	serine/threonine-protein kinase WNK4 isoform X1
XM_024980893.1	XP_024836661.1	serine/threonine-protein kinase WNK4 isoform X7
XM_003583605.4	XP_003583653.2	serine/threonine-protein kinase WNK4 isoform X3
XM_003587441.4	XP_003587489.1	serine/threonine-protein kinase WNK4 isoform X3
XM_024980889.1	XP_024836657.1	serine/threonine-protein kinase WNK4 isoform X2
XM_005220804.4	XP_005220861.1	serine/threonine-protein kinase WNK4 isoform X3
XM_005220806.3	XP_005220863.1	serine/threonine-protein kinase WNK4 isoform X4
XM_005195753.3	XP_005195810.2	serine/threonine-protein kinase WNK4 isoform X2
XM_024980891.1	XP_024836659.1	serine/threonine-protein kinase WNK4 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226908	XM_024980893.1 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa226904	XM_024980888.1 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226905	XM_024980889.1 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226906	XM_024980891.1 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa226907	XM_024980892.1 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa30079	XM_005220804.4 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30079	XM_005195752.3 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30080	XM_005220805.3 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30080	XM_005195753.3 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30081	XM_003583605.4 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30081	XM_003587441.5 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30082	XM_005220806.3 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30082	XM_005195754.3 Latest version!	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OBa30081	XM_003587441.4	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa30079	XM_005220804.3	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

WNK4 ( XM_024980893.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836661.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa226908
Clone ID Related Accession (Same CDS sequence)	XM_024980893.1
Accession Version	XM_024980893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGACCCAC 
ACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836661.1
CDS	122..3070
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESETHTL

Target ORF information:

RefSeq Version	XM_024980893.1
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980893.1

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGACCCAC 
ACTTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_024980888.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836656.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226904
Clone ID Related Accession (Same CDS sequence)	XM_024980888.1
Accession Version	XM_024980888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGTGAGA CAGAAGTCAC CAGCTCCCAT TGTTCCAGAG TCCCCTCCCT AGTGATCTGT 
CTTTCAGGCC CTGGGAATCT GCCCCTTGCT GTGGGGGTAC TAGCCCCCCA CTTTTCCCTG 
TGGCCCCTTC CCTCATTCAG TGCTCTCCCT CCCCATCCTG CACCCAGGCA TCATGCGAAG 
GAACTCCCTG AGTGGCAGCA GCACCGGCTC CCAGGAGCAG CGGGCAAGCA AGGGGGTGAC 
ATTCGCCGGG GATGTTGGCA GGATGTGAAT TATGAACAGA AGCCATGTGT CTCACCCACA 
CCTGGGCCCA CCATGGAGCT TATGCCCTTA GAATCTGCTG ACCAACACAA CTCTGCGAGG 
AAGACCTCAG CACTGAGGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836656.1
CDS	120..4103
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGETEVTSSHCSRVPSLVICLSGPGNLPLAVGVLAPHFSLWPLPSFSALPPHPAPRHHAKELPEWQQHRLPGAAGKQGGDIRRGCWQDVNYEQKPCVSPTPGPTMELMPLESADQHNSARKTSALRE

Target ORF information:

RefSeq Version	XM_024980888.1
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980888.1

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGTGAGA CAGAAGTCAC CAGCTCCCAT TGTTCCAGAG TCCCCTCCCT AGTGATCTGT 
CTTTCAGGCC CTGGGAATCT GCCCCTTGCT GTGGGGGTAC TAGCCCCCCA CTTTTCCCTG 
TGGCCCCTTC CCTCATTCAG TGCTCTCCCT CCCCATCCTG CACCCAGGCA TCATGCGAAG 
GAACTCCCTG AGTGGCAGCA GCACCGGCTC CCAGGAGCAG CGGGCAAGCA AGGGGGTGAC 
ATTCGCCGGG GATGTTGGCA GGATGTGAAT TATGAACAGA AGCCATGTGT CTCACCCACA 
CCTGGGCCCA CCATGGAGCT TATGCCCTTA GAATCTGCTG ACCAACACAA CTCTGCGAGG 
AAGACCTCAG CACTGAGGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_024980889.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836657.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

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CloneID	OBa226905
Clone ID Related Accession (Same CDS sequence)	XM_024980889.1
Accession Version	XM_024980889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC 
ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT 
CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC 
TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT 
CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG 
AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC 
TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC 
TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC 
CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT 
CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA 
AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG 
CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC 
CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC 
TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG 
GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT 
GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG 
ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG 
GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG 
GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG 
CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC 
AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG 
GGTGAGACAG AAGTCACCAG CTCCCATTGT TCCAGAGTCC CCTCCCTAGT GATCTGTCTT 
TCAGGCCCTG GGAATCTGCC CCTTGCTGTG GGGGTACTAG CCCCCCACTT TTCCCTGTGG 
CCCCTTCCCT CATTCAGTGC TCTCCCTCCC CATCCTGCAC CCAGGCATCA TGCGAAGGAA 
CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG GCAAGCAAGG GGGTGACATT 
CGCCGGGGAT GTTGGCAGGA TGTGAATTAT GAACAGAAGC CATGTGTCTC ACCCACACCT 
GGGCCCACCA TGGAGCTTAT GCCCTTAGAA TCTGCTGACC AACACAACTC TGCGAGGAAG 
ACCTCAGCAC TGAGGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836657.1
CDS	120..4100
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGETEVTSSHCSRVPSLVICLSGPGNLPLAVGVLAPHFSLWPLPSFSALPPHPAPRHHAKELPEWQQHRLPGAAGKQGGDIRRGCWQDVNYEQKPCVSPTPGPTMELMPLESADQHNSARKTSALRE

Target ORF information:

RefSeq Version	XM_024980889.1
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980889.1

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC 
ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT 
CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC 
TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT 
CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG 
AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC 
TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC 
TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC 
CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT 
CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA 
AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG 
CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC 
CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC 
TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG 
GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT 
GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG 
ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG 
GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG 
GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG 
CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC 
AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG 
GGTGAGACAG AAGTCACCAG CTCCCATTGT TCCAGAGTCC CCTCCCTAGT GATCTGTCTT 
TCAGGCCCTG GGAATCTGCC CCTTGCTGTG GGGGTACTAG CCCCCCACTT TTCCCTGTGG 
CCCCTTCCCT CATTCAGTGC TCTCCCTCCC CATCCTGCAC CCAGGCATCA TGCGAAGGAA 
CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG GCAAGCAAGG GGGTGACATT 
CGCCGGGGAT GTTGGCAGGA TGTGAATTAT GAACAGAAGC CATGTGTCTC ACCCACACCT 
GGGCCCACCA TGGAGCTTAT GCCCTTAGAA TCTGCTGACC AACACAACTC TGCGAGGAAG 
ACCTCAGCAC TGAGGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_024980891.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836659.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa226906
Clone ID Related Accession (Same CDS sequence)	XM_024980891.1
Accession Version	XM_024980891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC 
ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT 
CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC 
TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT 
CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG 
AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC 
TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC 
TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC 
CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT 
CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA 
AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG 
CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC 
CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC 
TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG 
GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT 
GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG 
ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG 
GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG 
GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG 
CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC 
AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG 
GGCATCATGC GAAGGAACTC CCTGAGTGGC AGCAGCACCG GCTCCCAGGA GCAGCGGGCA 
AGCAAGGGGG TGACATTCGC CGGGGATGTT GGCAGGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836659.1
CDS	120..3821
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM

Target ORF information:

RefSeq Version	XM_024980891.1
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980891.1

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC 
ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT 
CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC 
TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT 
CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG 
AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC 
TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC 
TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC 
CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT 
CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA 
AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG 
CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC 
CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC 
TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG 
GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT 
GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG 
ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG 
GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG 
GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG 
CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC 
AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG 
GGCATCATGC GAAGGAACTC CCTGAGTGGC AGCAGCACCG GCTCCCAGGA GCAGCGGGCA 
AGCAAGGGGG TGACATTCGC CGGGGATGTT GGCAGGATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_024980892.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836660.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa226907
Clone ID Related Accession (Same CDS sequence)	XM_024980892.1
Accession Version	XM_024980892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC GAGTCCTTCA GCGCTGGAGC CGCCAGATCC TGCGGGGGCT CCATTTCCTA 
CACTCCCGAG TACCCCCTAT CCTGCACCGA GACCTCAAGT GTGACAACGT CTTTATCACC 
GGCCCTTCGG GCTCCGTTAA GATCGGGGAC CTGGGCCTGG CCACGCTCAA GCGCGCCTCC 
TTCGCCAAGA GCGTCATCGG GACCCCGGAG TTCATGGCCC CAGAGATGTA CGAGGAAAAG 
TACGATGAGG CCGTGGACGT GTACGCGTTT GGCATGTGCA TGCTGGAGAT GGCGACCTCG 
GAGTACCCCT ACTCAGAGTG CCAGAATGCC GCTCAAATCT ACCGCAAGGT CACCTCGGGC 
ACGAAACCCA ACAGCTTCTA CAAGGTGAAG ATGCCCGAGG TAAAGGAGAT CATTGAAGGC 
TGCATCCGCA CGGATAAGAA TGAGAGGTTC ACCATCCACG ACCTTCTGGC TCACGCCTTC 
TTCCGCGAGG AGCGCGGCGT GCACGTGGAG TTGGCGGAGG AGGACGACGG AGAGAAGCCG 
GACCTCAAGC TCTGGCTGCG CATGGAGGAC GCGCGGCGAG GGGGGCGCCC ACGGGACAAC 
CAGGCCATCG AGTTCTTGTT CCAGTTGGGC CGGGACGCGG CAGAGGAGGT GGCTCAGGAG 
ATGGTGGCCC TGGGCTTAGT GTGTGAAGCT GATTACCAGC CAGTGGCTCG TGCAGTGCGT 
GAACGGGTTG CTGCCATCCA GCGAAAGCGT GAGAAGCTGC GTAAAGCTAG GGAGTTGGAG 
GCCCTCCCCC CAGCGCCGAG ACCCCCACCA GCAGCTGTCC CCATGACTCC TGGTCCCTCC 
AGTGCCTTCC CTCCTGAGCC CGAGGAGCCA GAGGCAGACC AGCACCAGCC CTTCCTCTTC 
CGCCATGCCA GCTATTCATC TACCACCTCG GATTGCGAGA CTGATGGCTA CCTCAGCTCT 
TCCGGCTTCC TGGATGCCTC AGACCCTGCC CTTCAGCCCC CTAGTGGGGT GCCATCCAGC 
CCCGCTGAGT CCCATCTCCG CCTGCCCGCG GCTTTTGCTC TATCCATTCC ACGTTCTGGC 
CCCGGCAATG ACTTTTCCCC TGGAGAGAGT TATGCCTCAG AGGCAGCATC AGGCCTTAGT 
GATGTGGGAG AAGGGATGGA ACGGATGAGG AGACCCCCAG GGAAAAACCT CCGGCGTAGA 
CCTCGATCCC GGCTTCGGGT CACAAGTGTC TCAGACCAGA ATGACAGAGT GGTTGAATGC 
CAGCTGCAGA CGCACAACAG CAAGATGGTG ACCTTCCGAT TTGATCTGGA TGGGGACAGC 
CCAGAAGAGA TCGCAGCTGC CATGGTGTAC AATGAGTTCA TTCTACCCTC GGAGCGAGCT 
GGATTCCTGA ACCGGATTCG GGAGATTATC CAGCGAGTGG AGACCCTGTT GAAGAGAGAT 
ACTGGCCCTG TGGAGGCTGC TGAAGACCCT CTGAGTCCCC AGGAGGAGTC AGCACCACTG 
CCTGCTCTCC CGGGGTCCCC CCCAGACCCA TCCAGGGCAG AGCTCCAGAG CAGCACCTCC 
CTGGAGCAAA GATGCTGGGC AGCTTTTTCC ACCTCCACAT CTTCTCCTGG AACCCCCTTG 
TCTCCTGGAA GCTCCTTTTC TCCTGGAACT CCTGTTTTCC CAAGTCCCAT CCTTCCCATC 
ACTTCTCCCC CATGTCATCT CAACCCCTCC TCATTCTCCC CAATTTCTCC TCAGGCCTCC 
TCAAATCTCT CTCCACACCC CCAAAGCTGT CCACTTCCAC TCTCCCCCAG GGCACCCCAG 
TTTCCAGTCC CATCTGCTCA GTTTCCACAG AGTTCTCTCT TCCCCGATTG TTTCCAGGGC 
ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC TCTGCTTGTC CCCTCCCCTC CACCACCGCA 
GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC TCTCTGGCTG TGATGACCGT GGCTCAGTCC 
CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC 
CCTAGCCTGC CCCCTCCCAC TCCCTGTACT CCTTGTGGCC AGGAGTGGCC TTCATCTCTG 
ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA AATCTTGGTC AGCCACTCCC GGGTGAAGCC 
AGATTGGCGC CCATCTCGAA AGAGGGAAAG CCTCAGCTTG TTGGGCGCTT CCAAGTGACT 
TCATCCAAGG AGCCAGCTGA GCCTCTTCCC CTGCAGCTGG CATCCCCAAC TCCCTCTGGC 
TCCCTGAAGC CTCCAACCCC TCAGCTGACC TCGGAGAGCT CGGACACAGA GGACAGTGCT 
GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA 
GGGGCTGGAG CTGAAGAGGA AGGGGACGAT GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT 
CCACCCCTGA GCCATCCTAG CCCAGTGTGG ATGAGCTACT CCTATAGCAG CCTGTGTCTG 
AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG GACGAGGAAT TCTGGGCTGA GCTGCAGAAT 
CTTCGGCAGA AGCACTTGTC AGAGGTGGAG GCACTACAGA CACTACAGAA ACAGGAAATC 
GAGGACTTGT ATAGCAGGCT TGGAAAGCAG CCCCCACCAG GGATCGTGGC CCCTGCTGCT 
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC ACGGCGCAAC 
AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG GGTGAGACAG AAGTCACCAG CTCCCATTGT 
TCCAGAGTCC CCTCCCTAGT GATCTGTCTT TCAGGCCCTG GGAATCTGCC CCTTGCTGTG 
GGGGTACTAG CCCCCCACTT TTCCCTGTGG CCCCTTCCCT CATTCAGTGC TCTCCCTCCC 
CATCCTGCAC CCAGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA 
GGAGCAGCGG GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGAATTAT 
GAACAGAAGC CATGTGTCTC ACCCACACCT GGGCCCACCA TGGAGCTTAT GCCCTTAGAA 
TCTGCTGACC AACACAACTC TGCGAGGAAG ACCTCAGCAC TGAGGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836660.1
CDS	136..3306
Translation	MKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGETEVTSSHCSRVPSLVICLSGPGNLPLAVGVLAPHFSLWPLPSFSALPPHPAPRHHAKELPEWQQHRLPGAAGKQGGDIRRGCWQDVNYEQKPCVSPTPGPTMELMPLESADQHNSARKTSALRE

Target ORF information:

RefSeq Version	XM_024980892.1
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980892.1

ORF Insert Sequence:

ATGAAACCAC GAGTCCTTCA GCGCTGGAGC CGCCAGATCC TGCGGGGGCT CCATTTCCTA 
CACTCCCGAG TACCCCCTAT CCTGCACCGA GACCTCAAGT GTGACAACGT CTTTATCACC 
GGCCCTTCGG GCTCCGTTAA GATCGGGGAC CTGGGCCTGG CCACGCTCAA GCGCGCCTCC 
TTCGCCAAGA GCGTCATCGG GACCCCGGAG TTCATGGCCC CAGAGATGTA CGAGGAAAAG 
TACGATGAGG CCGTGGACGT GTACGCGTTT GGCATGTGCA TGCTGGAGAT GGCGACCTCG 
GAGTACCCCT ACTCAGAGTG CCAGAATGCC GCTCAAATCT ACCGCAAGGT CACCTCGGGC 
ACGAAACCCA ACAGCTTCTA CAAGGTGAAG ATGCCCGAGG TAAAGGAGAT CATTGAAGGC 
TGCATCCGCA CGGATAAGAA TGAGAGGTTC ACCATCCACG ACCTTCTGGC TCACGCCTTC 
TTCCGCGAGG AGCGCGGCGT GCACGTGGAG TTGGCGGAGG AGGACGACGG AGAGAAGCCG 
GACCTCAAGC TCTGGCTGCG CATGGAGGAC GCGCGGCGAG GGGGGCGCCC ACGGGACAAC 
CAGGCCATCG AGTTCTTGTT CCAGTTGGGC CGGGACGCGG CAGAGGAGGT GGCTCAGGAG 
ATGGTGGCCC TGGGCTTAGT GTGTGAAGCT GATTACCAGC CAGTGGCTCG TGCAGTGCGT 
GAACGGGTTG CTGCCATCCA GCGAAAGCGT GAGAAGCTGC GTAAAGCTAG GGAGTTGGAG 
GCCCTCCCCC CAGCGCCGAG ACCCCCACCA GCAGCTGTCC CCATGACTCC TGGTCCCTCC 
AGTGCCTTCC CTCCTGAGCC CGAGGAGCCA GAGGCAGACC AGCACCAGCC CTTCCTCTTC 
CGCCATGCCA GCTATTCATC TACCACCTCG GATTGCGAGA CTGATGGCTA CCTCAGCTCT 
TCCGGCTTCC TGGATGCCTC AGACCCTGCC CTTCAGCCCC CTAGTGGGGT GCCATCCAGC 
CCCGCTGAGT CCCATCTCCG CCTGCCCGCG GCTTTTGCTC TATCCATTCC ACGTTCTGGC 
CCCGGCAATG ACTTTTCCCC TGGAGAGAGT TATGCCTCAG AGGCAGCATC AGGCCTTAGT 
GATGTGGGAG AAGGGATGGA ACGGATGAGG AGACCCCCAG GGAAAAACCT CCGGCGTAGA 
CCTCGATCCC GGCTTCGGGT CACAAGTGTC TCAGACCAGA ATGACAGAGT GGTTGAATGC 
CAGCTGCAGA CGCACAACAG CAAGATGGTG ACCTTCCGAT TTGATCTGGA TGGGGACAGC 
CCAGAAGAGA TCGCAGCTGC CATGGTGTAC AATGAGTTCA TTCTACCCTC GGAGCGAGCT 
GGATTCCTGA ACCGGATTCG GGAGATTATC CAGCGAGTGG AGACCCTGTT GAAGAGAGAT 
ACTGGCCCTG TGGAGGCTGC TGAAGACCCT CTGAGTCCCC AGGAGGAGTC AGCACCACTG 
CCTGCTCTCC CGGGGTCCCC CCCAGACCCA TCCAGGGCAG AGCTCCAGAG CAGCACCTCC 
CTGGAGCAAA GATGCTGGGC AGCTTTTTCC ACCTCCACAT CTTCTCCTGG AACCCCCTTG 
TCTCCTGGAA GCTCCTTTTC TCCTGGAACT CCTGTTTTCC CAAGTCCCAT CCTTCCCATC 
ACTTCTCCCC CATGTCATCT CAACCCCTCC TCATTCTCCC CAATTTCTCC TCAGGCCTCC 
TCAAATCTCT CTCCACACCC CCAAAGCTGT CCACTTCCAC TCTCCCCCAG GGCACCCCAG 
TTTCCAGTCC CATCTGCTCA GTTTCCACAG AGTTCTCTCT TCCCCGATTG TTTCCAGGGC 
ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC TCTGCTTGTC CCCTCCCCTC CACCACCGCA 
GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC TCTCTGGCTG TGATGACCGT GGCTCAGTCC 
CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC 
CCTAGCCTGC CCCCTCCCAC TCCCTGTACT CCTTGTGGCC AGGAGTGGCC TTCATCTCTG 
ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA AATCTTGGTC AGCCACTCCC GGGTGAAGCC 
AGATTGGCGC CCATCTCGAA AGAGGGAAAG CCTCAGCTTG TTGGGCGCTT CCAAGTGACT 
TCATCCAAGG AGCCAGCTGA GCCTCTTCCC CTGCAGCTGG CATCCCCAAC TCCCTCTGGC 
TCCCTGAAGC CTCCAACCCC TCAGCTGACC TCGGAGAGCT CGGACACAGA GGACAGTGCT 
GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA 
GGGGCTGGAG CTGAAGAGGA AGGGGACGAT GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT 
CCACCCCTGA GCCATCCTAG CCCAGTGTGG ATGAGCTACT CCTATAGCAG CCTGTGTCTG 
AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG GACGAGGAAT TCTGGGCTGA GCTGCAGAAT 
CTTCGGCAGA AGCACTTGTC AGAGGTGGAG GCACTACAGA CACTACAGAA ACAGGAAATC 
GAGGACTTGT ATAGCAGGCT TGGAAAGCAG CCCCCACCAG GGATCGTGGC CCCTGCTGCT 
ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC AAGGGCAGCT TCCCCACCTC ACGGCGCAAC 
AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG GGTGAGACAG AAGTCACCAG CTCCCATTGT 
TCCAGAGTCC CCTCCCTAGT GATCTGTCTT TCAGGCCCTG GGAATCTGCC CCTTGCTGTG 
GGGGTACTAG CCCCCCACTT TTCCCTGTGG CCCCTTCCCT CATTCAGTGC TCTCCCTCCC 
CATCCTGCAC CCAGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA 
GGAGCAGCGG GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGAATTAT 
GAACAGAAGC CATGTGTCTC ACCCACACCT GGGCCCACCA TGGAGCTTAT GCCCTTAGAA 
TCTGCTGACC AACACAACTC TGCGAGGAAG ACCTCAGCAC TGAGGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_005195752.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195809.2 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30079
Clone ID Related Accession (Same CDS sequence)	XM_005195752.3 , XM_005220804.3 , XM_005220804.4
Accession Version	XM_005195752.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195752.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT 
ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195809.2
CDS	113..3835
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPVNYEQKPCVSPTPGPTMELMPLESADQHNSARKTSALRE

Target ORF information:

RefSeq Version	XM_005195752.3
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195752.3

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT 
ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_005220804.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220861.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30079
Clone ID Related Accession (Same CDS sequence)	XM_005195752.3 , XM_005220804.3 , XM_005220804.4
Accession Version	XM_005220804.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220804.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT 
ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220861.1
CDS	113..3835
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPVNYEQKPCVSPTPGPTMELMPLESADQHNSARKTSALRE

Target ORF information:

RefSeq Version	XM_005220804.3
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220804.3

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT 
ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_005220804.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220861.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

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CloneID	OBa30079
Clone ID Related Accession (Same CDS sequence)	XM_005195752.3 , XM_005220804.3 , XM_005220804.4
Accession Version	XM_005220804.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220804.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT 
ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220861.1
CDS	120..3842
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPVNYEQKPCVSPTPGPTMELMPLESADQHNSARKTSALRE

Target ORF information:

RefSeq Version	XM_005220804.4
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220804.4

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGTGAATT ATGAACAGAA GCCATGTGTC TCACCCACAC CTGGGCCCAC CATGGAGCTT 
ATGCCCTTAG AATCTGCTGA CCAACACAAC TCTGCGAGGA AGACCTCAGC ACTGAGGGAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_005220805.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220862.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30080
Clone ID Related Accession (Same CDS sequence)	XM_005220805.3 , XM_005195753.3
Accession Version	XM_005220805.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220805.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC 
ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT 
CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC 
TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT 
CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG 
AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC 
TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC 
TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC 
CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT 
CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA 
AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG 
CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC 
CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC 
TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG 
GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT 
GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG 
ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG 
GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG 
GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG 
CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC 
AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG 
GTGAATTATG AACAGAAGCC ATGTGTCTCA CCCACACCTG GGCCCACCAT GGAGCTTATG 
CCCTTAGAAT CTGCTGACCA ACACAACTCT GCGAGGAAGA CCTCAGCACT GAGGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220862.1
CDS	113..3832
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPVNYEQKPCVSPTPGPTMELMPLESADQHNSARKTSALRE

Target ORF information:

RefSeq Version	XM_005220805.3
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220805.3

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC 
ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT 
CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC 
TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT 
CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG 
AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC 
TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC 
TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC 
CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT 
CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA 
AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG 
CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC 
CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC 
TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG 
GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT 
GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG 
ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG 
GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG 
GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG 
CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC 
AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG 
GTGAATTATG AACAGAAGCC ATGTGTCTCA CCCACACCTG GGCCCACCAT GGAGCTTATG 
CCCTTAGAAT CTGCTGACCA ACACAACTCT GCGAGGAAGA CCTCAGCACT GAGGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_005195753.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195810.2 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30080
Clone ID Related Accession (Same CDS sequence)	XM_005220805.3 , XM_005195753.3
Accession Version	XM_005195753.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195753.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC 
ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT 
CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC 
TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT 
CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG 
AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC 
TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC 
TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC 
CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT 
CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA 
AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG 
CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC 
CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC 
TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG 
GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT 
GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG 
ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG 
GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG 
GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG 
CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC 
AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG 
GTGAATTATG AACAGAAGCC ATGTGTCTCA CCCACACCTG GGCCCACCAT GGAGCTTATG 
CCCTTAGAAT CTGCTGACCA ACACAACTCT GCGAGGAAGA CCTCAGCACT GAGGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195810.2
CDS	113..3832
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPVNYEQKPCVSPTPGPTMELMPLESADQHNSARKTSALRE

Target ORF information:

RefSeq Version	XM_005195753.3
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195753.3

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG AGCTCCAGAG CAGCACCTCC CTGGAGCAAA GATGCTGGGC AGCTTTTTCC 
ACCTCCACAT CTTCTCCTGG AACCCCCTTG TCTCCTGGAA GCTCCTTTTC TCCTGGAACT 
CCTGTTTTCC CAAGTCCCAT CCTTCCCATC ACTTCTCCCC CATGTCATCT CAACCCCTCC 
TCATTCTCCC CAATTTCTCC TCAGGCCTCC TCAAATCTCT CTCCACACCC CCAAAGCTGT 
CCACTTCCAC TCTCCCCCAG GGCACCCCAG TTTCCAGTCC CATCTGCTCA GTTTCCACAG 
AGTTCTCTCT TCCCCGATTG TTTCCAGGGC ACTCCTGCTC CCTCCTTTCC CCTCTGCCCC 
TCTGCTTGTC CCCTCCCCTC CACCACCGCA GCCCCTCTCC TCTCTCTGGC TAGTGCCTTC 
TCTCTGGCTG TGATGACCGT GGCTCAGTCC CTGCTGTCCC CGTCCCCTGG GCTCCTGTCC 
CAGTCTCCTC CAGCTCCTCC TGGCCCTCTC CCTAGCCTGC CCCCTCCCAC TCCCTGTACT 
CCTTGTGGCC AGGAGTGGCC TTCATCTCTG ACAGCTGAGG TGGAGAGTGA GGCCTCTCCA 
AATCTTGGTC AGCCACTCCC GGGTGAAGCC AGATTGGCGC CCATCTCGAA AGAGGGAAAG 
CCTCAGCTTG TTGGGCGCTT CCAAGTGACT TCATCCAAGG AGCCAGCTGA GCCTCTTCCC 
CTGCAGCTGG CATCCCCAAC TCCCTCTGGC TCCCTGAAGC CTCCAACCCC TCAGCTGACC 
TCGGAGAGCT CGGACACAGA GGACAGTGCT GGAGCCAGGC CAGAGGCCAG GGAGGCTCTG 
GCTGAAAGTG ACCGTGCAGC CGAGGGCCTA GGGGCTGGAG CTGAAGAGGA AGGGGACGAT 
GGGCAGGAAC CCCAAGTAGG GGGCAGCCCT CCACCCCTGA GCCATCCTAG CCCAGTGTGG 
ATGAGCTACT CCTATAGCAG CCTGTGTCTG AGCAGTGAGG AGTCCGAGAG CAGTGGAGAG 
GACGAGGAAT TCTGGGCTGA GCTGCAGAAT CTTCGGCAGA AGCACTTGTC AGAGGTGGAG 
GCACTACAGA CACTACAGAA ACAGGAAATC GAGGACTTGT ATAGCAGGCT TGGAAAGCAG 
CCCCCACCAG GGATCGTGGC CCCTGCTGCT ATGCTGTCCA GCCGCCAGCG CCGCCTCTCC 
AAGGGCAGCT TCCCCACCTC ACGGCGCAAC AGCCTGCAGC GTTCTGAGCC CCTGGGCCCG 
GTGAATTATG AACAGAAGCC ATGTGTCTCA CCCACACCTG GGCCCACCAT GGAGCTTATG 
CCCTTAGAAT CTGCTGACCA ACACAACTCT GCGAGGAAGA CCTCAGCACT GAGGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_003587441.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587489.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

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CloneID	OBa30081
Clone ID Related Accession (Same CDS sequence)	XM_003587441.5 , XM_003583605.4 , XM_003587441.4
Accession Version	XM_003587441.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587441.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587489.1
CDS	120..3824
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM

Target ORF information:

RefSeq Version	XM_003587441.5
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587441.5

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_003583605.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583653.2 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30081
Clone ID Related Accession (Same CDS sequence)	XM_003587441.5 , XM_003583605.4 , XM_003587441.4
Accession Version	XM_003583605.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583605.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583653.2
CDS	1..3705
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM

Target ORF information:

RefSeq Version	XM_003583605.4
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583605.4

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_003587441.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587489.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30081
Clone ID Related Accession (Same CDS sequence)	XM_003587441.5 , XM_003583605.4 , XM_003587441.4
Accession Version	XM_003587441.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587441.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587489.1
CDS	1..3705
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGIMRRNSLSGSSTGSQEQRASKGVTFAGDVGRM

Target ORF information:

RefSeq Version	XM_003587441.4
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587441.4

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
GCAAGCAAGG GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_005195754.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195811.2 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

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CloneID	OBa30082
Clone ID Related Accession (Same CDS sequence)	XM_005195754.3 , XM_005220806.3
Accession Version	XM_005195754.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195811.2
CDS	113..3775
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGIMRRNSLSGSSTGSQEQR

Target ORF information:

RefSeq Version	XM_005195754.3
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195754.3

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WNK4 ( XM_005220806.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220863.1 Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

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CloneID	OBa30082
Clone ID Related Accession (Same CDS sequence)	XM_005195754.3 , XM_005220806.3
Accession Version	XM_005220806.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein kinase WNK4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220806.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220863.1
CDS	113..3775
Translation	MLAPPAPETEVPMSQAEADLPLRPPPTLAAAGPPRLGPPSRRVRRFSGKAEPRPRSSRLSRRSSVDLGLLSSWSQPASPVPEPPDPPDSAGSGPAMSPPPSSEEPLEGMWTTGAPVKPADSERLEPAGSTGGSGSREQPRITEAAAARERRREQEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMPEVKEIIEGCIRTDKNERFTIHDLLAHAFFREERGVHVELAEEDDGEKPDLKLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVRERVAAIQRKREKLRKARELEALPPAPRPPPAAVPMTPGPSSAFPPEPEEPEADQHQPFLFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPSGVPSSPAESHLRLPAAFALSIPRSGPGNDFSPGESYASEAASGLSDVGEGMERMRRPPGKNLRRRPRSRLRVTSVSDQNDRVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERAGFLNRIREIIQRVETLLKRDTGPVEAAEDPLSPQEESAPLPALPGSPPDPSRAELQSSTSLEQRCWAAFSTSTSSPGTPLSPGSSFSPGTPVFPSPILPITSPPCHLNPSSFSPISPQASSNLSPHPQSCPLPLSPRAPQFPVPSAQFPQSSLFPDCFQGTPAPSFPLCPSACPLPSTTAAPLLSLASAFSLAVMTVAQSLLSPSPGLLSQSPPAPPGPLPSLPPPTPCTPCGQEWPSSLTAEVESEASPNLGQPLPGEARLAPISKEGKPQLVGRFQVTSSKEPAEPLPLQLASPTPSGSLKPPTPQLTSESSDTEDSAGARPEAREALAESDRAAEGLGAGAEEEGDDGQEPQVGGSPPPLSHPSPVWMSYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKQEIEDLYSRLGKQPPPGIVAPAAMLSSRQRRLSKGSFPTSRRNSLQRSEPLGPGIMRRNSLSGSSTGSQEQR

Target ORF information:

RefSeq Version	XM_005220806.3
Organism	Bos taurus(cattle)
Definition	Bos taurus WNK lysine deficient protein kinase 4 (WNK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220806.3

ORF Insert Sequence:

ATGCTGGCAC CCCCGGCCCC AGAGACCGAA GTCCCCATGT CCCAGGCGGA GGCTGACCTG 
CCCCTGCGGC CCCCGCCGAC CCTTGCCGCG GCGGGGCCGC CCCGCCTCGG GCCCCCTTCC 
CGCCGGGTGC GCCGCTTCTC CGGGAAGGCT GAGCCCCGGC CGCGCTCTTC CCGTCTCAGC 
CGCCGCAGCT CAGTCGACTT GGGGCTACTG AGCTCTTGGT CCCAGCCAGC CTCACCCGTT 
CCGGAGCCCC CTGATCCGCC GGACTCCGCT GGTTCCGGCC CCGCGATGAG CCCACCGCCC 
AGCTCTGAAG AGCCCCTTGA GGGCATGTGG ACCACGGGCG CCCCTGTGAA GCCTGCAGAC 
TCCGAGCGTT TGGAGCCCGC CGGCTCCACA GGAGGGTCGG GGTCCCGGGA ACAGCCGAGG 
ATCACCGAGG CGGCGGCGGC CCGGGAGCGG CGGCGGGAGC AAGAGGAAAA AGAGGACACG 
GAGACCCAGG CTGTGGCAAC GTCCCCGGAC GGCCGATACC TCAAGTTTGA CATCGAGATT 
GGACGTGGCT CGTTCAAGAC GGTGTATCGA GGGCTGGACA CCGACACCAC GGTGGAGGTG 
GCCTGGTGTG AGCTGCAGAC TCGGAAACTG TCTCGAGCCG AGCGGCAGCG ATTCTCAGAG 
GAGGTGGAGA TGCTCAAGGG GCTGCAGCAC CCCAACATCG TCCGCTTCTA CGACTCCTGG 
AAGTCGGTGC TGAGGGGCCA GGTTTGCATC GTGCTGGTCA CCGAACTCAT GACCTCGGGC 
ACGCTCAAGA CATACCTGAG GCGGTTCCGT GAGATGAAAC CACGAGTCCT TCAGCGCTGG 
AGCCGCCAGA TCCTGCGGGG GCTCCATTTC CTACACTCCC GAGTACCCCC TATCCTGCAC 
CGAGACCTCA AGTGTGACAA CGTCTTTATC ACCGGCCCTT CGGGCTCCGT TAAGATCGGG 
GACCTGGGCC TGGCCACGCT CAAGCGCGCC TCCTTCGCCA AGAGCGTCAT CGGGACCCCG 
GAGTTCATGG CCCCAGAGAT GTACGAGGAA AAGTACGATG AGGCCGTGGA CGTGTACGCG 
TTTGGCATGT GCATGCTGGA GATGGCGACC TCGGAGTACC CCTACTCAGA GTGCCAGAAT 
GCCGCTCAAA TCTACCGCAA GGTCACCTCG GGCACGAAAC CCAACAGCTT CTACAAGGTG 
AAGATGCCCG AGGTAAAGGA GATCATTGAA GGCTGCATCC GCACGGATAA GAATGAGAGG 
TTCACCATCC ACGACCTTCT GGCTCACGCC TTCTTCCGCG AGGAGCGCGG CGTGCACGTG 
GAGTTGGCGG AGGAGGACGA CGGAGAGAAG CCGGACCTCA AGCTCTGGCT GCGCATGGAG 
GACGCGCGGC GAGGGGGGCG CCCACGGGAC AACCAGGCCA TCGAGTTCTT GTTCCAGTTG 
GGCCGGGACG CGGCAGAGGA GGTGGCTCAG GAGATGGTGG CCCTGGGCTT AGTGTGTGAA 
GCTGATTACC AGCCAGTGGC TCGTGCAGTG CGTGAACGGG TTGCTGCCAT CCAGCGAAAG 
CGTGAGAAGC TGCGTAAAGC TAGGGAGTTG GAGGCCCTCC CCCCAGCGCC GAGACCCCCA 
CCAGCAGCTG TCCCCATGAC TCCTGGTCCC TCCAGTGCCT TCCCTCCTGA GCCCGAGGAG 
CCAGAGGCAG ACCAGCACCA GCCCTTCCTC TTCCGCCATG CCAGCTATTC ATCTACCACC 
TCGGATTGCG AGACTGATGG CTACCTCAGC TCTTCCGGCT TCCTGGATGC CTCAGACCCT 
GCCCTTCAGC CCCCTAGTGG GGTGCCATCC AGCCCCGCTG AGTCCCATCT CCGCCTGCCC 
GCGGCTTTTG CTCTATCCAT TCCACGTTCT GGCCCCGGCA ATGACTTTTC CCCTGGAGAG 
AGTTATGCCT CAGAGGCAGC ATCAGGCCTT AGTGATGTGG GAGAAGGGAT GGAACGGATG 
AGGAGACCCC CAGGGAAAAA CCTCCGGCGT AGACCTCGAT CCCGGCTTCG GGTCACAAGT 
GTCTCAGACC AGAATGACAG AGTGGTTGAA TGCCAGCTGC AGACGCACAA CAGCAAGATG 
GTGACCTTCC GATTTGATCT GGATGGGGAC AGCCCAGAAG AGATCGCAGC TGCCATGGTG 
TACAATGAGT TCATTCTACC CTCGGAGCGA GCTGGATTCC TGAACCGGAT TCGGGAGATT 
ATCCAGCGAG TGGAGACCCT GTTGAAGAGA GATACTGGCC CTGTGGAGGC TGCTGAAGAC 
CCTCTGAGTC CCCAGGAGGA GTCAGCACCA CTGCCTGCTC TCCCGGGGTC CCCCCCAGAC 
CCATCCAGGG CAGAGCTCCA GAGCAGCACC TCCCTGGAGC AAAGATGCTG GGCAGCTTTT 
TCCACCTCCA CATCTTCTCC TGGAACCCCC TTGTCTCCTG GAAGCTCCTT TTCTCCTGGA 
ACTCCTGTTT TCCCAAGTCC CATCCTTCCC ATCACTTCTC CCCCATGTCA TCTCAACCCC 
TCCTCATTCT CCCCAATTTC TCCTCAGGCC TCCTCAAATC TCTCTCCACA CCCCCAAAGC 
TGTCCACTTC CACTCTCCCC CAGGGCACCC CAGTTTCCAG TCCCATCTGC TCAGTTTCCA 
CAGAGTTCTC TCTTCCCCGA TTGTTTCCAG GGCACTCCTG CTCCCTCCTT TCCCCTCTGC 
CCCTCTGCTT GTCCCCTCCC CTCCACCACC GCAGCCCCTC TCCTCTCTCT GGCTAGTGCC 
TTCTCTCTGG CTGTGATGAC CGTGGCTCAG TCCCTGCTGT CCCCGTCCCC TGGGCTCCTG 
TCCCAGTCTC CTCCAGCTCC TCCTGGCCCT CTCCCTAGCC TGCCCCCTCC CACTCCCTGT 
ACTCCTTGTG GCCAGGAGTG GCCTTCATCT CTGACAGCTG AGGTGGAGAG TGAGGCCTCT 
CCAAATCTTG GTCAGCCACT CCCGGGTGAA GCCAGATTGG CGCCCATCTC GAAAGAGGGA 
AAGCCTCAGC TTGTTGGGCG CTTCCAAGTG ACTTCATCCA AGGAGCCAGC TGAGCCTCTT 
CCCCTGCAGC TGGCATCCCC AACTCCCTCT GGCTCCCTGA AGCCTCCAAC CCCTCAGCTG 
ACCTCGGAGA GCTCGGACAC AGAGGACAGT GCTGGAGCCA GGCCAGAGGC CAGGGAGGCT 
CTGGCTGAAA GTGACCGTGC AGCCGAGGGC CTAGGGGCTG GAGCTGAAGA GGAAGGGGAC 
GATGGGCAGG AACCCCAAGT AGGGGGCAGC CCTCCACCCC TGAGCCATCC TAGCCCAGTG 
TGGATGAGCT ACTCCTATAG CAGCCTGTGT CTGAGCAGTG AGGAGTCCGA GAGCAGTGGA 
GAGGACGAGG AATTCTGGGC TGAGCTGCAG AATCTTCGGC AGAAGCACTT GTCAGAGGTG 
GAGGCACTAC AGACACTACA GAAACAGGAA ATCGAGGACT TGTATAGCAG GCTTGGAAAG 
CAGCCCCCAC CAGGGATCGT GGCCCCTGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC 
TCCAAGGGCA GCTTCCCCAC CTCACGGCGC AACAGCCTGC AGCGTTCTGA GCCCCTGGGC 
CCGGGCATCA TGCGAAGGAA CTCCCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WNK4
Entrez Gene ID	100849076
Full Name	WNK lysine deficient protein kinase 4
General protein information	Preferred Names WNK lysine deficient protein kinase 4 Names serine/threonine-protein kinase WNK4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Gallus gallus (chicken)	LOC777580	XP_003642875.2
Pan troglodytes (chimpanzee)	WNK4	XP_003315535.1
Rattus norvegicus (Norway rat)	Wnk4	NP_783169.2
Oryza sativa (rice)	Os12g0162100	NP_001066222.1
Bos taurus (cattle)	WNK4	XP_003587489.1
Mus musculus (house mouse)	Wnk4	NP_783569.1
Oryza sativa (rice)	Os11g0160300	NP_001065816.1
Homo sapiens (human)	WNK4	NP_115763.2
	LOC707820	XP_002808177.1
Canis lupus familiaris (dog)	WNK4	XP_548082.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WNK4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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