BBS1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BBS1
Entrez Gene ID	100848046
Full Name	Bardet-Biedl syndrome 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005197361.3	XP_005197418.1	Bardet-Biedl syndrome 1 protein isoform X2
XM_010821174.3	XP_010819476.1	Bardet-Biedl syndrome 1 protein isoform X1
XM_010821174.2	XP_010819476.1	Bardet-Biedl syndrome 1 protein isoform X1
XM_005227234.3	XP_005227291.1	Bardet-Biedl syndrome 1 protein isoform X2
XM_010826341.2	XP_010824643.1	Bardet-Biedl syndrome 1 protein isoform X1
XM_005227234.4	XP_005227291.1	Bardet-Biedl syndrome 1 protein isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	BBS1	NP_078925.3
Canis lupus familiaris (dog)	BBS1	XP_540830.2
Bos taurus (cattle)	BBS1	XP_003588094.2
Mus musculus (house mouse)	Bbs1	NP_001028300.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007459	XP_308406.4
Pan troglodytes (chimpanzee)	BBS1	XP_001171950.1
Rattus norvegicus (Norway rat)	Bbs1	NP_001101039.1
Danio rerio (zebrafish)	bbs1	NP_001091721.1
Drosophila melanogaster (fruit fly)	BBS1	NP_648080.1
Caenorhabditis elegans (roundworm)	bbs-1	NP_740933.1
Xenopus tropicalis (tropical clawed frog)	bbs1	NP_001116276.1

Gene Ontology

The following BBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa12780	XM_010826341.2 Latest version!	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa12780	XM_010821174.3 Latest version!	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa12781	XM_005197361.3 Latest version!	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa12781	XM_005227234.4 Latest version!	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa12780	XM_010821174.2	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa12781	XM_005227234.3	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BBS1 ( XM_010821174.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819476.1 Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa12780
Clone ID Related Accession (Same CDS sequence)	XM_010821174.3 , XM_010821174.2 , XM_010826341.2
Accession Version	XM_010821174.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Bardet-Biedl syndrome 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010821174.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGCAGTGAGG CCAATTCGAA ATGGCTGGAC 
TCGCTCAGCG ATTCCATGGC GAACATCCAC ACCTTTTCCG CCTGCCTGGC GCTGGCCGAT 
TTTCACGGGG ACGGGGAGTA CAAGCTGGCC ATGGGAGACC TTGGCCCAGA TGGGCGGCAG 
CCCCGCCTGA AGGTGCTTAA AGGACACACA CTGGTGAGCC AGAAACCGCT ACCCGACCTG 
CCAGCCGCTG CTGTCACCTT CCTCATGGCG TCACACGAGC CCCGGACTCC AGCACTGGCA 
ATCGCATCAG GCCCTTGTGT GTATGTGTAT AAGAATCTCA AGCCCTACTT CAAGTTCAGC 
CTGCCCTCGC TGCCTACAAA CCCCCTGGAA CAAGACCTTT GGAATCAGGC CAAAGAGGAC 
CAGATTGACC CCTTGACCCT GAAGGAGATG CTGGAGGGCA TCAGGGAGAA GGCAGAGGTG 
CCTTTGTCTG TACAGTCACT CAGGTTTCTG CCGCTGGAGC TGAGTGAAAT GGAGGCGTTT 
GTGAACCAGC ACAAATCCAA GTCCATCAGG CGGCAGACAG TCATCACCAC CATGACCACC 
TTAAAGAAGA ACCTGGCTGA CGAGGACGCT GTGTCCTGCC TGGTGCTGGG CACTGAGAAC 
AAGGAGCTCC TGGTGCTAGA CCCGGAGGCC TTCACCATCC TGGCCAAGAT GAGCCTCCCC 
AGCGTCCCTG CCTTCCTGGA GGCTTCTGGC CAGTTTGATG TGGAGTTCCG TCTTGCCGCT 
GCCTGCCGCA ACGGGAGCAT CTATATACTG AGAAGAGACT CCAAGCGTCC CAAGTACTGC 
ATCGAGCTGG GCGCCCAGCC TGTGGGGCTT GTCGGTGTAC ACAAAGTCCT GGTGGTGGGC 
AGCAACCAAG ACAGCCTGCA CGGCTTCACC TACAAGGGTA AGAGGCTGTG GACAGTGCAG 
ATGCCTGCAG CCATCCTGGC CATGAACCTC CTGGAGCAGC ACTCCCGGGG CCTGCAGGCC 
GTCATGGCTG CGCTGGCCAA CGAAGAGGTC CGCATTTACC ATGACAAGGT CCTACTCAAC 
GTCATCCGCA CGCCGGAGGC AGTGACCAGC CTTTGCTTTG GCCGCTATGG GCGGGAGGAT 
AATACCCTTA TCATGACTAC ACTAGGTGGT GGCCTGATCA TCAAGATCCT CAAGCGCACA 
GCGGTGTTTG CCGAGGGGGG CGGTGAGGCC GGCCCCCCAC CAAGCCAGGC CATAAAACTC 
AACGTGCCCC GGAAGACCCG GCTGTACGTG GACCAGACAC TGCGAGAGCG GGAAGCCGGT 
ACCGCCATGC ACCGGACCTT CCAGGCGGAC CTCTACCTGC TGCGCCTCCG GGCGGCCCGT 
GCCTACGTGC AGGCCCTTGA GTCCAGCCTG AGCCCCGTGT CTCTGACGGC CCGGGAGCCG 
CTCAAGCTGC ACGCTGTGGT CCAGGGCCTG GGCCCCACCT TCAAGCTCAC CCTTCACCTG 
CAGAACACAT CAACAGCCCG CCCCATCCTG GGGCTAGTGG TCTGCTTCCT GTACAACGAG 
GTGCTCTATG CTCTTCCCCG GGCCTTCTTC AAGGTCCCAT TGCTGGTGCC AGGGCTCAAC 
TACCCCCTGG AGACCTTTGT GAAGAGTCTT AGTGACAAGG GCATCTCAGA CATCATCAAG 
GTGCTGGTGC TTCGAGAAGG CCAGAGCACA CCCCTGCTGA GTGCCCACAT CAACATGCCC 
ATGAGTGAGG GGCTGGCAGC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819476.1
CDS	543..2549
Translation	MGRIRDKLYKSERGIASQLVETPARTSATCSGASLSRAGKLSARNLDTRASPQPGSFWALRGGWPQPVARVTPGKMAATSSSDSDGGKGESSEANSKWLDSLSDSMANIHTFSACLALADFHGDGEYKLAMGDLGPDGRQPRLKVLKGHTLVSQKPLPDLPAAAVTFLMASHEPRTPALAIASGPCVYVYKNLKPYFKFSLPSLPTNPLEQDLWNQAKEDQIDPLTLKEMLEGIREKAEVPLSVQSLRFLPLELSEMEAFVNQHKSKSIRRQTVITTMTTLKKNLADEDAVSCLVLGTENKELLVLDPEAFTILAKMSLPSVPAFLEASGQFDVEFRLAAACRNGSIYILRRDSKRPKYCIELGAQPVGLVGVHKVLVVGSNQDSLHGFTYKGKRLWTVQMPAAILAMNLLEQHSRGLQAVMAALANEEVRIYHDKVLLNVIRTPEAVTSLCFGRYGREDNTLIMTTLGGGLIIKILKRTAVFAEGGGEAGPPPSQAIKLNVPRKTRLYVDQTLREREAGTAMHRTFQADLYLLRLRAARAYVQALESSLSPVSLTAREPLKLHAVVQGLGPTFKLTLHLQNTSTARPILGLVVCFLYNEVLYALPRAFFKVPLLVPGLNYPLETFVKSLSDKGISDIIKVLVLREGQSTPLLSAHINMPMSEGLAAD

Target ORF information:

RefSeq Version	XM_010821174.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010821174.3

ORF Insert Sequence:

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGCAGTGAGG CCAATTCGAA ATGGCTGGAC 
TCGCTCAGCG ATTCCATGGC GAACATCCAC ACCTTTTCCG CCTGCCTGGC GCTGGCCGAT 
TTTCACGGGG ACGGGGAGTA CAAGCTGGCC ATGGGAGACC TTGGCCCAGA TGGGCGGCAG 
CCCCGCCTGA AGGTGCTTAA AGGACACACA CTGGTGAGCC AGAAACCGCT ACCCGACCTG 
CCAGCCGCTG CTGTCACCTT CCTCATGGCG TCACACGAGC CCCGGACTCC AGCACTGGCA 
ATCGCATCAG GCCCTTGTGT GTATGTGTAT AAGAATCTCA AGCCCTACTT CAAGTTCAGC 
CTGCCCTCGC TGCCTACAAA CCCCCTGGAA CAAGACCTTT GGAATCAGGC CAAAGAGGAC 
CAGATTGACC CCTTGACCCT GAAGGAGATG CTGGAGGGCA TCAGGGAGAA GGCAGAGGTG 
CCTTTGTCTG TACAGTCACT CAGGTTTCTG CCGCTGGAGC TGAGTGAAAT GGAGGCGTTT 
GTGAACCAGC ACAAATCCAA GTCCATCAGG CGGCAGACAG TCATCACCAC CATGACCACC 
TTAAAGAAGA ACCTGGCTGA CGAGGACGCT GTGTCCTGCC TGGTGCTGGG CACTGAGAAC 
AAGGAGCTCC TGGTGCTAGA CCCGGAGGCC TTCACCATCC TGGCCAAGAT GAGCCTCCCC 
AGCGTCCCTG CCTTCCTGGA GGCTTCTGGC CAGTTTGATG TGGAGTTCCG TCTTGCCGCT 
GCCTGCCGCA ACGGGAGCAT CTATATACTG AGAAGAGACT CCAAGCGTCC CAAGTACTGC 
ATCGAGCTGG GCGCCCAGCC TGTGGGGCTT GTCGGTGTAC ACAAAGTCCT GGTGGTGGGC 
AGCAACCAAG ACAGCCTGCA CGGCTTCACC TACAAGGGTA AGAGGCTGTG GACAGTGCAG 
ATGCCTGCAG CCATCCTGGC CATGAACCTC CTGGAGCAGC ACTCCCGGGG CCTGCAGGCC 
GTCATGGCTG CGCTGGCCAA CGAAGAGGTC CGCATTTACC ATGACAAGGT CCTACTCAAC 
GTCATCCGCA CGCCGGAGGC AGTGACCAGC CTTTGCTTTG GCCGCTATGG GCGGGAGGAT 
AATACCCTTA TCATGACTAC ACTAGGTGGT GGCCTGATCA TCAAGATCCT CAAGCGCACA 
GCGGTGTTTG CCGAGGGGGG CGGTGAGGCC GGCCCCCCAC CAAGCCAGGC CATAAAACTC 
AACGTGCCCC GGAAGACCCG GCTGTACGTG GACCAGACAC TGCGAGAGCG GGAAGCCGGT 
ACCGCCATGC ACCGGACCTT CCAGGCGGAC CTCTACCTGC TGCGCCTCCG GGCGGCCCGT 
GCCTACGTGC AGGCCCTTGA GTCCAGCCTG AGCCCCGTGT CTCTGACGGC CCGGGAGCCG 
CTCAAGCTGC ACGCTGTGGT CCAGGGCCTG GGCCCCACCT TCAAGCTCAC CCTTCACCTG 
CAGAACACAT CAACAGCCCG CCCCATCCTG GGGCTAGTGG TCTGCTTCCT GTACAACGAG 
GTGCTCTATG CTCTTCCCCG GGCCTTCTTC AAGGTCCCAT TGCTGGTGCC AGGGCTCAAC 
TACCCCCTGG AGACCTTTGT GAAGAGTCTT AGTGACAAGG GCATCTCAGA CATCATCAAG 
GTGCTGGTGC TTCGAGAAGG CCAGAGCACA CCCCTGCTGA GTGCCCACAT CAACATGCCC 
ATGAGTGAGG GGCTGGCAGC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BBS1 ( XM_010821174.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819476.1 Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa12780
Clone ID Related Accession (Same CDS sequence)	XM_010821174.3 , XM_010821174.2 , XM_010826341.2
Accession Version	XM_010821174.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	Bardet-Biedl syndrome 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010821174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGCAGTGAGG CCAATTCGAA ATGGCTGGAC 
TCGCTCAGCG ATTCCATGGC GAACATCCAC ACCTTTTCCG CCTGCCTGGC GCTGGCCGAT 
TTTCACGGGG ACGGGGAGTA CAAGCTGGCC ATGGGAGACC TTGGCCCAGA TGGGCGGCAG 
CCCCGCCTGA AGGTGCTTAA AGGACACACA CTGGTGAGCC AGAAACCGCT ACCCGACCTG 
CCAGCCGCTG CTGTCACCTT CCTCATGGCG TCACACGAGC CCCGGACTCC AGCACTGGCA 
ATCGCATCAG GCCCTTGTGT GTATGTGTAT AAGAATCTCA AGCCCTACTT CAAGTTCAGC 
CTGCCCTCGC TGCCTACAAA CCCCCTGGAA CAAGACCTTT GGAATCAGGC CAAAGAGGAC 
CAGATTGACC CCTTGACCCT GAAGGAGATG CTGGAGGGCA TCAGGGAGAA GGCAGAGGTG 
CCTTTGTCTG TACAGTCACT CAGGTTTCTG CCGCTGGAGC TGAGTGAAAT GGAGGCGTTT 
GTGAACCAGC ACAAATCCAA GTCCATCAGG CGGCAGACAG TCATCACCAC CATGACCACC 
TTAAAGAAGA ACCTGGCTGA CGAGGACGCT GTGTCCTGCC TGGTGCTGGG CACTGAGAAC 
AAGGAGCTCC TGGTGCTAGA CCCGGAGGCC TTCACCATCC TGGCCAAGAT GAGCCTCCCC 
AGCGTCCCTG CCTTCCTGGA GGCTTCTGGC CAGTTTGATG TGGAGTTCCG TCTTGCCGCT 
GCCTGCCGCA ACGGGAGCAT CTATATACTG AGAAGAGACT CCAAGCGTCC CAAGTACTGC 
ATCGAGCTGG GCGCCCAGCC TGTGGGGCTT GTCGGTGTAC ACAAAGTCCT GGTGGTGGGC 
AGCAACCAAG ACAGCCTGCA CGGCTTCACC TACAAGGGTA AGAGGCTGTG GACAGTGCAG 
ATGCCTGCAG CCATCCTGGC CATGAACCTC CTGGAGCAGC ACTCCCGGGG CCTGCAGGCC 
GTCATGGCTG CGCTGGCCAA CGAAGAGGTC CGCATTTACC ATGACAAGGT CCTACTCAAC 
GTCATCCGCA CGCCGGAGGC AGTGACCAGC CTTTGCTTTG GCCGCTATGG GCGGGAGGAT 
AATACCCTTA TCATGACTAC ACTAGGTGGT GGCCTGATCA TCAAGATCCT CAAGCGCACA 
GCGGTGTTTG CCGAGGGGGG CGGTGAGGCC GGCCCCCCAC CAAGCCAGGC CATAAAACTC 
AACGTGCCCC GGAAGACCCG GCTGTACGTG GACCAGACAC TGCGAGAGCG GGAAGCCGGT 
ACCGCCATGC ACCGGACCTT CCAGGCGGAC CTCTACCTGC TGCGCCTCCG GGCGGCCCGT 
GCCTACGTGC AGGCCCTTGA GTCCAGCCTG AGCCCCGTGT CTCTGACGGC CCGGGAGCCG 
CTCAAGCTGC ACGCTGTGGT CCAGGGCCTG GGCCCCACCT TCAAGCTCAC CCTTCACCTG 
CAGAACACAT CAACAGCCCG CCCCATCCTG GGGCTAGTGG TCTGCTTCCT GTACAACGAG 
GTGCTCTATG CTCTTCCCCG GGCCTTCTTC AAGGTCCCAT TGCTGGTGCC AGGGCTCAAC 
TACCCCCTGG AGACCTTTGT GAAGAGTCTT AGTGACAAGG GCATCTCAGA CATCATCAAG 
GTGCTGGTGC TTCGAGAAGG CCAGAGCACA CCCCTGCTGA GTGCCCACAT CAACATGCCC 
ATGAGTGAGG GGCTGGCAGC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819476.1
CDS	530..2536
Translation	MGRIRDKLYKSERGIASQLVETPARTSATCSGASLSRAGKLSARNLDTRASPQPGSFWALRGGWPQPVARVTPGKMAATSSSDSDGGKGESSEANSKWLDSLSDSMANIHTFSACLALADFHGDGEYKLAMGDLGPDGRQPRLKVLKGHTLVSQKPLPDLPAAAVTFLMASHEPRTPALAIASGPCVYVYKNLKPYFKFSLPSLPTNPLEQDLWNQAKEDQIDPLTLKEMLEGIREKAEVPLSVQSLRFLPLELSEMEAFVNQHKSKSIRRQTVITTMTTLKKNLADEDAVSCLVLGTENKELLVLDPEAFTILAKMSLPSVPAFLEASGQFDVEFRLAAACRNGSIYILRRDSKRPKYCIELGAQPVGLVGVHKVLVVGSNQDSLHGFTYKGKRLWTVQMPAAILAMNLLEQHSRGLQAVMAALANEEVRIYHDKVLLNVIRTPEAVTSLCFGRYGREDNTLIMTTLGGGLIIKILKRTAVFAEGGGEAGPPPSQAIKLNVPRKTRLYVDQTLREREAGTAMHRTFQADLYLLRLRAARAYVQALESSLSPVSLTAREPLKLHAVVQGLGPTFKLTLHLQNTSTARPILGLVVCFLYNEVLYALPRAFFKVPLLVPGLNYPLETFVKSLSDKGISDIIKVLVLREGQSTPLLSAHINMPMSEGLAAD

Target ORF information:

RefSeq Version	XM_010821174.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010821174.2

ORF Insert Sequence:

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGCAGTGAGG CCAATTCGAA ATGGCTGGAC 
TCGCTCAGCG ATTCCATGGC GAACATCCAC ACCTTTTCCG CCTGCCTGGC GCTGGCCGAT 
TTTCACGGGG ACGGGGAGTA CAAGCTGGCC ATGGGAGACC TTGGCCCAGA TGGGCGGCAG 
CCCCGCCTGA AGGTGCTTAA AGGACACACA CTGGTGAGCC AGAAACCGCT ACCCGACCTG 
CCAGCCGCTG CTGTCACCTT CCTCATGGCG TCACACGAGC CCCGGACTCC AGCACTGGCA 
ATCGCATCAG GCCCTTGTGT GTATGTGTAT AAGAATCTCA AGCCCTACTT CAAGTTCAGC 
CTGCCCTCGC TGCCTACAAA CCCCCTGGAA CAAGACCTTT GGAATCAGGC CAAAGAGGAC 
CAGATTGACC CCTTGACCCT GAAGGAGATG CTGGAGGGCA TCAGGGAGAA GGCAGAGGTG 
CCTTTGTCTG TACAGTCACT CAGGTTTCTG CCGCTGGAGC TGAGTGAAAT GGAGGCGTTT 
GTGAACCAGC ACAAATCCAA GTCCATCAGG CGGCAGACAG TCATCACCAC CATGACCACC 
TTAAAGAAGA ACCTGGCTGA CGAGGACGCT GTGTCCTGCC TGGTGCTGGG CACTGAGAAC 
AAGGAGCTCC TGGTGCTAGA CCCGGAGGCC TTCACCATCC TGGCCAAGAT GAGCCTCCCC 
AGCGTCCCTG CCTTCCTGGA GGCTTCTGGC CAGTTTGATG TGGAGTTCCG TCTTGCCGCT 
GCCTGCCGCA ACGGGAGCAT CTATATACTG AGAAGAGACT CCAAGCGTCC CAAGTACTGC 
ATCGAGCTGG GCGCCCAGCC TGTGGGGCTT GTCGGTGTAC ACAAAGTCCT GGTGGTGGGC 
AGCAACCAAG ACAGCCTGCA CGGCTTCACC TACAAGGGTA AGAGGCTGTG GACAGTGCAG 
ATGCCTGCAG CCATCCTGGC CATGAACCTC CTGGAGCAGC ACTCCCGGGG CCTGCAGGCC 
GTCATGGCTG CGCTGGCCAA CGAAGAGGTC CGCATTTACC ATGACAAGGT CCTACTCAAC 
GTCATCCGCA CGCCGGAGGC AGTGACCAGC CTTTGCTTTG GCCGCTATGG GCGGGAGGAT 
AATACCCTTA TCATGACTAC ACTAGGTGGT GGCCTGATCA TCAAGATCCT CAAGCGCACA 
GCGGTGTTTG CCGAGGGGGG CGGTGAGGCC GGCCCCCCAC CAAGCCAGGC CATAAAACTC 
AACGTGCCCC GGAAGACCCG GCTGTACGTG GACCAGACAC TGCGAGAGCG GGAAGCCGGT 
ACCGCCATGC ACCGGACCTT CCAGGCGGAC CTCTACCTGC TGCGCCTCCG GGCGGCCCGT 
GCCTACGTGC AGGCCCTTGA GTCCAGCCTG AGCCCCGTGT CTCTGACGGC CCGGGAGCCG 
CTCAAGCTGC ACGCTGTGGT CCAGGGCCTG GGCCCCACCT TCAAGCTCAC CCTTCACCTG 
CAGAACACAT CAACAGCCCG CCCCATCCTG GGGCTAGTGG TCTGCTTCCT GTACAACGAG 
GTGCTCTATG CTCTTCCCCG GGCCTTCTTC AAGGTCCCAT TGCTGGTGCC AGGGCTCAAC 
TACCCCCTGG AGACCTTTGT GAAGAGTCTT AGTGACAAGG GCATCTCAGA CATCATCAAG 
GTGCTGGTGC TTCGAGAAGG CCAGAGCACA CCCCTGCTGA GTGCCCACAT CAACATGCCC 
ATGAGTGAGG GGCTGGCAGC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BBS1 ( XM_010826341.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824643.1 Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa12780
Clone ID Related Accession (Same CDS sequence)	XM_010821174.3 , XM_010821174.2 , XM_010826341.2
Accession Version	XM_010826341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	Bardet-Biedl syndrome 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646807.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGCAGTGAGG CCAATTCGAA ATGGCTGGAC 
TCGCTCAGCG ATTCCATGGC GAACATCCAC ACCTTTTCCG CCTGCCTGGC GCTGGCCGAT 
TTTCACGGGG ACGGGGAGTA CAAGCTGGCC ATGGGAGACC TTGGCCCAGA TGGGCGGCAG 
CCCCGCCTGA AGGTGCTTAA AGGACACACA CTGGTGAGCC AGAAACCGCT ACCCGACCTG 
CCAGCCGCTG CTGTCACCTT CCTCATGGCG TCACACGAGC CCCGGACTCC AGCACTGGCA 
ATCGCATCAG GCCCTTGTGT GTATGTGTAT AAGAATCTCA AGCCCTACTT CAAGTTCAGC 
CTGCCCTCGC TGCCTACAAA CCCCCTGGAA CAAGACCTTT GGAATCAGGC CAAAGAGGAC 
CAGATTGACC CCTTGACCCT GAAGGAGATG CTGGAGGGCA TCAGGGAGAA GGCAGAGGTG 
CCTTTGTCTG TACAGTCACT CAGGTTTCTG CCGCTGGAGC TGAGTGAAAT GGAGGCGTTT 
GTGAACCAGC ACAAATCCAA GTCCATCAGG CGGCAGACAG TCATCACCAC CATGACCACC 
TTAAAGAAGA ACCTGGCTGA CGAGGACGCT GTGTCCTGCC TGGTGCTGGG CACTGAGAAC 
AAGGAGCTCC TGGTGCTAGA CCCGGAGGCC TTCACCATCC TGGCCAAGAT GAGCCTCCCC 
AGCGTCCCTG CCTTCCTGGA GGCTTCTGGC CAGTTTGATG TGGAGTTCCG TCTTGCCGCT 
GCCTGCCGCA ACGGGAGCAT CTATATACTG AGAAGAGACT CCAAGCGTCC CAAGTACTGC 
ATCGAGCTGG GCGCCCAGCC TGTGGGGCTT GTCGGTGTAC ACAAAGTCCT GGTGGTGGGC 
AGCAACCAAG ACAGCCTGCA CGGCTTCACC TACAAGGGTA AGAGGCTGTG GACAGTGCAG 
ATGCCTGCAG CCATCCTGGC CATGAACCTC CTGGAGCAGC ACTCCCGGGG CCTGCAGGCC 
GTCATGGCTG CGCTGGCCAA CGAAGAGGTC CGCATTTACC ATGACAAGGT CCTACTCAAC 
GTCATCCGCA CGCCGGAGGC AGTGACCAGC CTTTGCTTTG GCCGCTATGG GCGGGAGGAT 
AATACCCTTA TCATGACTAC ACTAGGTGGT GGCCTGATCA TCAAGATCCT CAAGCGCACA 
GCGGTGTTTG CCGAGGGGGG CGGTGAGGCC GGCCCCCCAC CAAGCCAGGC CATAAAACTC 
AACGTGCCCC GGAAGACCCG GCTGTACGTG GACCAGACAC TGCGAGAGCG GGAAGCCGGT 
ACCGCCATGC ACCGGACCTT CCAGGCGGAC CTCTACCTGC TGCGCCTCCG GGCGGCCCGT 
GCCTACGTGC AGGCCCTTGA GTCCAGCCTG AGCCCCGTGT CTCTGACGGC CCGGGAGCCG 
CTCAAGCTGC ACGCTGTGGT CCAGGGCCTG GGCCCCACCT TCAAGCTCAC CCTTCACCTG 
CAGAACACAT CAACAGCCCG CCCCATCCTG GGGCTAGTGG TCTGCTTCCT GTACAACGAG 
GTGCTCTATG CTCTTCCCCG GGCCTTCTTC AAGGTCCCAT TGCTGGTGCC AGGGCTCAAC 
TACCCCCTGG AGACCTTTGT GAAGAGTCTT AGTGACAAGG GCATCTCAGA CATCATCAAG 
GTGCTGGTGC TTCGAGAAGG CCAGAGCACA CCCCTGCTGA GTGCCCACAT CAACATGCCC 
ATGAGTGAGG GGCTGGCAGC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824643.1
CDS	530..2536
Translation	MGRIRDKLYKSERGIASQLVETPARTSATCSGASLSRAGKLSARNLDTRASPQPGSFWALRGGWPQPVARVTPGKMAATSSSDSDGGKGESSEANSKWLDSLSDSMANIHTFSACLALADFHGDGEYKLAMGDLGPDGRQPRLKVLKGHTLVSQKPLPDLPAAAVTFLMASHEPRTPALAIASGPCVYVYKNLKPYFKFSLPSLPTNPLEQDLWNQAKEDQIDPLTLKEMLEGIREKAEVPLSVQSLRFLPLELSEMEAFVNQHKSKSIRRQTVITTMTTLKKNLADEDAVSCLVLGTENKELLVLDPEAFTILAKMSLPSVPAFLEASGQFDVEFRLAAACRNGSIYILRRDSKRPKYCIELGAQPVGLVGVHKVLVVGSNQDSLHGFTYKGKRLWTVQMPAAILAMNLLEQHSRGLQAVMAALANEEVRIYHDKVLLNVIRTPEAVTSLCFGRYGREDNTLIMTTLGGGLIIKILKRTAVFAEGGGEAGPPPSQAIKLNVPRKTRLYVDQTLREREAGTAMHRTFQADLYLLRLRAARAYVQALESSLSPVSLTAREPLKLHAVVQGLGPTFKLTLHLQNTSTARPILGLVVCFLYNEVLYALPRAFFKVPLLVPGLNYPLETFVKSLSDKGISDIIKVLVLREGQSTPLLSAHINMPMSEGLAAD

Target ORF information:

RefSeq Version	XM_010826341.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826341.2

ORF Insert Sequence:

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGCAGTGAGG CCAATTCGAA ATGGCTGGAC 
TCGCTCAGCG ATTCCATGGC GAACATCCAC ACCTTTTCCG CCTGCCTGGC GCTGGCCGAT 
TTTCACGGGG ACGGGGAGTA CAAGCTGGCC ATGGGAGACC TTGGCCCAGA TGGGCGGCAG 
CCCCGCCTGA AGGTGCTTAA AGGACACACA CTGGTGAGCC AGAAACCGCT ACCCGACCTG 
CCAGCCGCTG CTGTCACCTT CCTCATGGCG TCACACGAGC CCCGGACTCC AGCACTGGCA 
ATCGCATCAG GCCCTTGTGT GTATGTGTAT AAGAATCTCA AGCCCTACTT CAAGTTCAGC 
CTGCCCTCGC TGCCTACAAA CCCCCTGGAA CAAGACCTTT GGAATCAGGC CAAAGAGGAC 
CAGATTGACC CCTTGACCCT GAAGGAGATG CTGGAGGGCA TCAGGGAGAA GGCAGAGGTG 
CCTTTGTCTG TACAGTCACT CAGGTTTCTG CCGCTGGAGC TGAGTGAAAT GGAGGCGTTT 
GTGAACCAGC ACAAATCCAA GTCCATCAGG CGGCAGACAG TCATCACCAC CATGACCACC 
TTAAAGAAGA ACCTGGCTGA CGAGGACGCT GTGTCCTGCC TGGTGCTGGG CACTGAGAAC 
AAGGAGCTCC TGGTGCTAGA CCCGGAGGCC TTCACCATCC TGGCCAAGAT GAGCCTCCCC 
AGCGTCCCTG CCTTCCTGGA GGCTTCTGGC CAGTTTGATG TGGAGTTCCG TCTTGCCGCT 
GCCTGCCGCA ACGGGAGCAT CTATATACTG AGAAGAGACT CCAAGCGTCC CAAGTACTGC 
ATCGAGCTGG GCGCCCAGCC TGTGGGGCTT GTCGGTGTAC ACAAAGTCCT GGTGGTGGGC 
AGCAACCAAG ACAGCCTGCA CGGCTTCACC TACAAGGGTA AGAGGCTGTG GACAGTGCAG 
ATGCCTGCAG CCATCCTGGC CATGAACCTC CTGGAGCAGC ACTCCCGGGG CCTGCAGGCC 
GTCATGGCTG CGCTGGCCAA CGAAGAGGTC CGCATTTACC ATGACAAGGT CCTACTCAAC 
GTCATCCGCA CGCCGGAGGC AGTGACCAGC CTTTGCTTTG GCCGCTATGG GCGGGAGGAT 
AATACCCTTA TCATGACTAC ACTAGGTGGT GGCCTGATCA TCAAGATCCT CAAGCGCACA 
GCGGTGTTTG CCGAGGGGGG CGGTGAGGCC GGCCCCCCAC CAAGCCAGGC CATAAAACTC 
AACGTGCCCC GGAAGACCCG GCTGTACGTG GACCAGACAC TGCGAGAGCG GGAAGCCGGT 
ACCGCCATGC ACCGGACCTT CCAGGCGGAC CTCTACCTGC TGCGCCTCCG GGCGGCCCGT 
GCCTACGTGC AGGCCCTTGA GTCCAGCCTG AGCCCCGTGT CTCTGACGGC CCGGGAGCCG 
CTCAAGCTGC ACGCTGTGGT CCAGGGCCTG GGCCCCACCT TCAAGCTCAC CCTTCACCTG 
CAGAACACAT CAACAGCCCG CCCCATCCTG GGGCTAGTGG TCTGCTTCCT GTACAACGAG 
GTGCTCTATG CTCTTCCCCG GGCCTTCTTC AAGGTCCCAT TGCTGGTGCC AGGGCTCAAC 
TACCCCCTGG AGACCTTTGT GAAGAGTCTT AGTGACAAGG GCATCTCAGA CATCATCAAG 
GTGCTGGTGC TTCGAGAAGG CCAGAGCACA CCCCTGCTGA GTGCCCACAT CAACATGCCC 
ATGAGTGAGG GGCTGGCAGC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BBS1 ( XM_005197361.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197418.1 Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa12781
Clone ID Related Accession (Same CDS sequence)	XM_005197361.3 , XM_005227234.3 , XM_005227234.4
Accession Version	XM_005197361.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	Bardet-Biedl syndrome 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646807.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGTGAGGCCA ATTCGAAATG GCTGGACTCG 
CTCAGCGATT CCATGGCGAA CATCCACACC TTTTCCGCCT GCCTGGCGCT GGCCGATTTT 
CACGGGGACG GGGAGTACAA GCTGGCCATG GGAGACCTTG GCCCAGATGG GCGGCAGCCC 
CGCCTGAAGG TGCTTAAAGG ACACACACTG GTGAGCCAGA AACCGCTACC CGACCTGCCA 
GCCGCTGCTG TCACCTTCCT CATGGCGTCA CACGAGCCCC GGACTCCAGC ACTGGCAATC 
GCATCAGGCC CTTGTGTGTA TGTGTATAAG AATCTCAAGC CCTACTTCAA GTTCAGCCTG 
CCCTCGCTGC CTACAAACCC CCTGGAACAA GACCTTTGGA ATCAGGCCAA AGAGGACCAG 
ATTGACCCCT TGACCCTGAA GGAGATGCTG GAGGGCATCA GGGAGAAGGC AGAGGTGCCT 
TTGTCTGTAC AGTCACTCAG GTTTCTGCCG CTGGAGCTGA GTGAAATGGA GGCGTTTGTG 
AACCAGCACA AATCCAAGTC CATCAGGCGG CAGACAGTCA TCACCACCAT GACCACCTTA 
AAGAAGAACC TGGCTGACGA GGACGCTGTG TCCTGCCTGG TGCTGGGCAC TGAGAACAAG 
GAGCTCCTGG TGCTAGACCC GGAGGCCTTC ACCATCCTGG CCAAGATGAG CCTCCCCAGC 
GTCCCTGCCT TCCTGGAGGC TTCTGGCCAG TTTGATGTGG AGTTCCGTCT TGCCGCTGCC 
TGCCGCAACG GGAGCATCTA TATACTGAGA AGAGACTCCA AGCGTCCCAA GTACTGCATC 
GAGCTGGGCG CCCAGCCTGT GGGGCTTGTC GGTGTACACA AAGTCCTGGT GGTGGGCAGC 
AACCAAGACA GCCTGCACGG CTTCACCTAC AAGGGTAAGA GGCTGTGGAC AGTGCAGATG 
CCTGCAGCCA TCCTGGCCAT GAACCTCCTG GAGCAGCACT CCCGGGGCCT GCAGGCCGTC 
ATGGCTGCGC TGGCCAACGA AGAGGTCCGC ATTTACCATG ACAAGGTCCT ACTCAACGTC 
ATCCGCACGC CGGAGGCAGT GACCAGCCTT TGCTTTGGCC GCTATGGGCG GGAGGATAAT 
ACCCTTATCA TGACTACACT AGGTGGTGGC CTGATCATCA AGATCCTCAA GCGCACAGCG 
GTGTTTGCCG AGGGGGGCGG TGAGGCCGGC CCCCCACCAA GCCAGGCCAT AAAACTCAAC 
GTGCCCCGGA AGACCCGGCT GTACGTGGAC CAGACACTGC GAGAGCGGGA AGCCGGTACC 
GCCATGCACC GGACCTTCCA GGCGGACCTC TACCTGCTGC GCCTCCGGGC GGCCCGTGCC 
TACGTGCAGG CCCTTGAGTC CAGCCTGAGC CCCGTGTCTC TGACGGCCCG GGAGCCGCTC 
AAGCTGCACG CTGTGGTCCA GGGCCTGGGC CCCACCTTCA AGCTCACCCT TCACCTGCAG 
AACACATCAA CAGCCCGCCC CATCCTGGGG CTAGTGGTCT GCTTCCTGTA CAACGAGGTG 
CTCTATGCTC TTCCCCGGGC CTTCTTCAAG GTCCCATTGC TGGTGCCAGG GCTCAACTAC 
CCCCTGGAGA CCTTTGTGAA GAGTCTTAGT GACAAGGGCA TCTCAGACAT CATCAAGGTG 
CTGGTGCTTC GAGAAGGCCA GAGCACACCC CTGCTGAGTG CCCACATCAA CATGCCCATG 
AGTGAGGGGC TGGCAGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197418.1
CDS	530..2533
Translation	MGRIRDKLYKSERGIASQLVETPARTSATCSGASLSRAGKLSARNLDTRASPQPGSFWALRGGWPQPVARVTPGKMAATSSSDSDGGKGESEANSKWLDSLSDSMANIHTFSACLALADFHGDGEYKLAMGDLGPDGRQPRLKVLKGHTLVSQKPLPDLPAAAVTFLMASHEPRTPALAIASGPCVYVYKNLKPYFKFSLPSLPTNPLEQDLWNQAKEDQIDPLTLKEMLEGIREKAEVPLSVQSLRFLPLELSEMEAFVNQHKSKSIRRQTVITTMTTLKKNLADEDAVSCLVLGTENKELLVLDPEAFTILAKMSLPSVPAFLEASGQFDVEFRLAAACRNGSIYILRRDSKRPKYCIELGAQPVGLVGVHKVLVVGSNQDSLHGFTYKGKRLWTVQMPAAILAMNLLEQHSRGLQAVMAALANEEVRIYHDKVLLNVIRTPEAVTSLCFGRYGREDNTLIMTTLGGGLIIKILKRTAVFAEGGGEAGPPPSQAIKLNVPRKTRLYVDQTLREREAGTAMHRTFQADLYLLRLRAARAYVQALESSLSPVSLTAREPLKLHAVVQGLGPTFKLTLHLQNTSTARPILGLVVCFLYNEVLYALPRAFFKVPLLVPGLNYPLETFVKSLSDKGISDIIKVLVLREGQSTPLLSAHINMPMSEGLAAD

Target ORF information:

RefSeq Version	XM_005197361.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197361.3

ORF Insert Sequence:

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGTGAGGCCA ATTCGAAATG GCTGGACTCG 
CTCAGCGATT CCATGGCGAA CATCCACACC TTTTCCGCCT GCCTGGCGCT GGCCGATTTT 
CACGGGGACG GGGAGTACAA GCTGGCCATG GGAGACCTTG GCCCAGATGG GCGGCAGCCC 
CGCCTGAAGG TGCTTAAAGG ACACACACTG GTGAGCCAGA AACCGCTACC CGACCTGCCA 
GCCGCTGCTG TCACCTTCCT CATGGCGTCA CACGAGCCCC GGACTCCAGC ACTGGCAATC 
GCATCAGGCC CTTGTGTGTA TGTGTATAAG AATCTCAAGC CCTACTTCAA GTTCAGCCTG 
CCCTCGCTGC CTACAAACCC CCTGGAACAA GACCTTTGGA ATCAGGCCAA AGAGGACCAG 
ATTGACCCCT TGACCCTGAA GGAGATGCTG GAGGGCATCA GGGAGAAGGC AGAGGTGCCT 
TTGTCTGTAC AGTCACTCAG GTTTCTGCCG CTGGAGCTGA GTGAAATGGA GGCGTTTGTG 
AACCAGCACA AATCCAAGTC CATCAGGCGG CAGACAGTCA TCACCACCAT GACCACCTTA 
AAGAAGAACC TGGCTGACGA GGACGCTGTG TCCTGCCTGG TGCTGGGCAC TGAGAACAAG 
GAGCTCCTGG TGCTAGACCC GGAGGCCTTC ACCATCCTGG CCAAGATGAG CCTCCCCAGC 
GTCCCTGCCT TCCTGGAGGC TTCTGGCCAG TTTGATGTGG AGTTCCGTCT TGCCGCTGCC 
TGCCGCAACG GGAGCATCTA TATACTGAGA AGAGACTCCA AGCGTCCCAA GTACTGCATC 
GAGCTGGGCG CCCAGCCTGT GGGGCTTGTC GGTGTACACA AAGTCCTGGT GGTGGGCAGC 
AACCAAGACA GCCTGCACGG CTTCACCTAC AAGGGTAAGA GGCTGTGGAC AGTGCAGATG 
CCTGCAGCCA TCCTGGCCAT GAACCTCCTG GAGCAGCACT CCCGGGGCCT GCAGGCCGTC 
ATGGCTGCGC TGGCCAACGA AGAGGTCCGC ATTTACCATG ACAAGGTCCT ACTCAACGTC 
ATCCGCACGC CGGAGGCAGT GACCAGCCTT TGCTTTGGCC GCTATGGGCG GGAGGATAAT 
ACCCTTATCA TGACTACACT AGGTGGTGGC CTGATCATCA AGATCCTCAA GCGCACAGCG 
GTGTTTGCCG AGGGGGGCGG TGAGGCCGGC CCCCCACCAA GCCAGGCCAT AAAACTCAAC 
GTGCCCCGGA AGACCCGGCT GTACGTGGAC CAGACACTGC GAGAGCGGGA AGCCGGTACC 
GCCATGCACC GGACCTTCCA GGCGGACCTC TACCTGCTGC GCCTCCGGGC GGCCCGTGCC 
TACGTGCAGG CCCTTGAGTC CAGCCTGAGC CCCGTGTCTC TGACGGCCCG GGAGCCGCTC 
AAGCTGCACG CTGTGGTCCA GGGCCTGGGC CCCACCTTCA AGCTCACCCT TCACCTGCAG 
AACACATCAA CAGCCCGCCC CATCCTGGGG CTAGTGGTCT GCTTCCTGTA CAACGAGGTG 
CTCTATGCTC TTCCCCGGGC CTTCTTCAAG GTCCCATTGC TGGTGCCAGG GCTCAACTAC 
CCCCTGGAGA CCTTTGTGAA GAGTCTTAGT GACAAGGGCA TCTCAGACAT CATCAAGGTG 
CTGGTGCTTC GAGAAGGCCA GAGCACACCC CTGCTGAGTG CCCACATCAA CATGCCCATG 
AGTGAGGGGC TGGCAGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BBS1 ( XM_005227234.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227291.1 Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa12781
Clone ID Related Accession (Same CDS sequence)	XM_005197361.3 , XM_005227234.3 , XM_005227234.4
Accession Version	XM_005227234.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	Bardet-Biedl syndrome 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005227234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGTGAGGCCA ATTCGAAATG GCTGGACTCG 
CTCAGCGATT CCATGGCGAA CATCCACACC TTTTCCGCCT GCCTGGCGCT GGCCGATTTT 
CACGGGGACG GGGAGTACAA GCTGGCCATG GGAGACCTTG GCCCAGATGG GCGGCAGCCC 
CGCCTGAAGG TGCTTAAAGG ACACACACTG GTGAGCCAGA AACCGCTACC CGACCTGCCA 
GCCGCTGCTG TCACCTTCCT CATGGCGTCA CACGAGCCCC GGACTCCAGC ACTGGCAATC 
GCATCAGGCC CTTGTGTGTA TGTGTATAAG AATCTCAAGC CCTACTTCAA GTTCAGCCTG 
CCCTCGCTGC CTACAAACCC CCTGGAACAA GACCTTTGGA ATCAGGCCAA AGAGGACCAG 
ATTGACCCCT TGACCCTGAA GGAGATGCTG GAGGGCATCA GGGAGAAGGC AGAGGTGCCT 
TTGTCTGTAC AGTCACTCAG GTTTCTGCCG CTGGAGCTGA GTGAAATGGA GGCGTTTGTG 
AACCAGCACA AATCCAAGTC CATCAGGCGG CAGACAGTCA TCACCACCAT GACCACCTTA 
AAGAAGAACC TGGCTGACGA GGACGCTGTG TCCTGCCTGG TGCTGGGCAC TGAGAACAAG 
GAGCTCCTGG TGCTAGACCC GGAGGCCTTC ACCATCCTGG CCAAGATGAG CCTCCCCAGC 
GTCCCTGCCT TCCTGGAGGC TTCTGGCCAG TTTGATGTGG AGTTCCGTCT TGCCGCTGCC 
TGCCGCAACG GGAGCATCTA TATACTGAGA AGAGACTCCA AGCGTCCCAA GTACTGCATC 
GAGCTGGGCG CCCAGCCTGT GGGGCTTGTC GGTGTACACA AAGTCCTGGT GGTGGGCAGC 
AACCAAGACA GCCTGCACGG CTTCACCTAC AAGGGTAAGA GGCTGTGGAC AGTGCAGATG 
CCTGCAGCCA TCCTGGCCAT GAACCTCCTG GAGCAGCACT CCCGGGGCCT GCAGGCCGTC 
ATGGCTGCGC TGGCCAACGA AGAGGTCCGC ATTTACCATG ACAAGGTCCT ACTCAACGTC 
ATCCGCACGC CGGAGGCAGT GACCAGCCTT TGCTTTGGCC GCTATGGGCG GGAGGATAAT 
ACCCTTATCA TGACTACACT AGGTGGTGGC CTGATCATCA AGATCCTCAA GCGCACAGCG 
GTGTTTGCCG AGGGGGGCGG TGAGGCCGGC CCCCCACCAA GCCAGGCCAT AAAACTCAAC 
GTGCCCCGGA AGACCCGGCT GTACGTGGAC CAGACACTGC GAGAGCGGGA AGCCGGTACC 
GCCATGCACC GGACCTTCCA GGCGGACCTC TACCTGCTGC GCCTCCGGGC GGCCCGTGCC 
TACGTGCAGG CCCTTGAGTC CAGCCTGAGC CCCGTGTCTC TGACGGCCCG GGAGCCGCTC 
AAGCTGCACG CTGTGGTCCA GGGCCTGGGC CCCACCTTCA AGCTCACCCT TCACCTGCAG 
AACACATCAA CAGCCCGCCC CATCCTGGGG CTAGTGGTCT GCTTCCTGTA CAACGAGGTG 
CTCTATGCTC TTCCCCGGGC CTTCTTCAAG GTCCCATTGC TGGTGCCAGG GCTCAACTAC 
CCCCTGGAGA CCTTTGTGAA GAGTCTTAGT GACAAGGGCA TCTCAGACAT CATCAAGGTG 
CTGGTGCTTC GAGAAGGCCA GAGCACACCC CTGCTGAGTG CCCACATCAA CATGCCCATG 
AGTGAGGGGC TGGCAGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227291.1
CDS	530..2533
Translation	MGRIRDKLYKSERGIASQLVETPARTSATCSGASLSRAGKLSARNLDTRASPQPGSFWALRGGWPQPVARVTPGKMAATSSSDSDGGKGESEANSKWLDSLSDSMANIHTFSACLALADFHGDGEYKLAMGDLGPDGRQPRLKVLKGHTLVSQKPLPDLPAAAVTFLMASHEPRTPALAIASGPCVYVYKNLKPYFKFSLPSLPTNPLEQDLWNQAKEDQIDPLTLKEMLEGIREKAEVPLSVQSLRFLPLELSEMEAFVNQHKSKSIRRQTVITTMTTLKKNLADEDAVSCLVLGTENKELLVLDPEAFTILAKMSLPSVPAFLEASGQFDVEFRLAAACRNGSIYILRRDSKRPKYCIELGAQPVGLVGVHKVLVVGSNQDSLHGFTYKGKRLWTVQMPAAILAMNLLEQHSRGLQAVMAALANEEVRIYHDKVLLNVIRTPEAVTSLCFGRYGREDNTLIMTTLGGGLIIKILKRTAVFAEGGGEAGPPPSQAIKLNVPRKTRLYVDQTLREREAGTAMHRTFQADLYLLRLRAARAYVQALESSLSPVSLTAREPLKLHAVVQGLGPTFKLTLHLQNTSTARPILGLVVCFLYNEVLYALPRAFFKVPLLVPGLNYPLETFVKSLSDKGISDIIKVLVLREGQSTPLLSAHINMPMSEGLAAD

Target ORF information:

RefSeq Version	XM_005227234.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227234.3

ORF Insert Sequence:

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGTGAGGCCA ATTCGAAATG GCTGGACTCG 
CTCAGCGATT CCATGGCGAA CATCCACACC TTTTCCGCCT GCCTGGCGCT GGCCGATTTT 
CACGGGGACG GGGAGTACAA GCTGGCCATG GGAGACCTTG GCCCAGATGG GCGGCAGCCC 
CGCCTGAAGG TGCTTAAAGG ACACACACTG GTGAGCCAGA AACCGCTACC CGACCTGCCA 
GCCGCTGCTG TCACCTTCCT CATGGCGTCA CACGAGCCCC GGACTCCAGC ACTGGCAATC 
GCATCAGGCC CTTGTGTGTA TGTGTATAAG AATCTCAAGC CCTACTTCAA GTTCAGCCTG 
CCCTCGCTGC CTACAAACCC CCTGGAACAA GACCTTTGGA ATCAGGCCAA AGAGGACCAG 
ATTGACCCCT TGACCCTGAA GGAGATGCTG GAGGGCATCA GGGAGAAGGC AGAGGTGCCT 
TTGTCTGTAC AGTCACTCAG GTTTCTGCCG CTGGAGCTGA GTGAAATGGA GGCGTTTGTG 
AACCAGCACA AATCCAAGTC CATCAGGCGG CAGACAGTCA TCACCACCAT GACCACCTTA 
AAGAAGAACC TGGCTGACGA GGACGCTGTG TCCTGCCTGG TGCTGGGCAC TGAGAACAAG 
GAGCTCCTGG TGCTAGACCC GGAGGCCTTC ACCATCCTGG CCAAGATGAG CCTCCCCAGC 
GTCCCTGCCT TCCTGGAGGC TTCTGGCCAG TTTGATGTGG AGTTCCGTCT TGCCGCTGCC 
TGCCGCAACG GGAGCATCTA TATACTGAGA AGAGACTCCA AGCGTCCCAA GTACTGCATC 
GAGCTGGGCG CCCAGCCTGT GGGGCTTGTC GGTGTACACA AAGTCCTGGT GGTGGGCAGC 
AACCAAGACA GCCTGCACGG CTTCACCTAC AAGGGTAAGA GGCTGTGGAC AGTGCAGATG 
CCTGCAGCCA TCCTGGCCAT GAACCTCCTG GAGCAGCACT CCCGGGGCCT GCAGGCCGTC 
ATGGCTGCGC TGGCCAACGA AGAGGTCCGC ATTTACCATG ACAAGGTCCT ACTCAACGTC 
ATCCGCACGC CGGAGGCAGT GACCAGCCTT TGCTTTGGCC GCTATGGGCG GGAGGATAAT 
ACCCTTATCA TGACTACACT AGGTGGTGGC CTGATCATCA AGATCCTCAA GCGCACAGCG 
GTGTTTGCCG AGGGGGGCGG TGAGGCCGGC CCCCCACCAA GCCAGGCCAT AAAACTCAAC 
GTGCCCCGGA AGACCCGGCT GTACGTGGAC CAGACACTGC GAGAGCGGGA AGCCGGTACC 
GCCATGCACC GGACCTTCCA GGCGGACCTC TACCTGCTGC GCCTCCGGGC GGCCCGTGCC 
TACGTGCAGG CCCTTGAGTC CAGCCTGAGC CCCGTGTCTC TGACGGCCCG GGAGCCGCTC 
AAGCTGCACG CTGTGGTCCA GGGCCTGGGC CCCACCTTCA AGCTCACCCT TCACCTGCAG 
AACACATCAA CAGCCCGCCC CATCCTGGGG CTAGTGGTCT GCTTCCTGTA CAACGAGGTG 
CTCTATGCTC TTCCCCGGGC CTTCTTCAAG GTCCCATTGC TGGTGCCAGG GCTCAACTAC 
CCCCTGGAGA CCTTTGTGAA GAGTCTTAGT GACAAGGGCA TCTCAGACAT CATCAAGGTG 
CTGGTGCTTC GAGAAGGCCA GAGCACACCC CTGCTGAGTG CCCACATCAA CATGCCCATG 
AGTGAGGGGC TGGCAGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BBS1 ( XM_005227234.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005227291.1 Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X3, mRNA.

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CloneID	OBa12781
Clone ID Related Accession (Same CDS sequence)	XM_005197361.3 , XM_005227234.3 , XM_005227234.4
Accession Version	XM_005227234.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Bardet-Biedl syndrome 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005227234.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGTGAGGCCA ATTCGAAATG GCTGGACTCG 
CTCAGCGATT CCATGGCGAA CATCCACACC TTTTCCGCCT GCCTGGCGCT GGCCGATTTT 
CACGGGGACG GGGAGTACAA GCTGGCCATG GGAGACCTTG GCCCAGATGG GCGGCAGCCC 
CGCCTGAAGG TGCTTAAAGG ACACACACTG GTGAGCCAGA AACCGCTACC CGACCTGCCA 
GCCGCTGCTG TCACCTTCCT CATGGCGTCA CACGAGCCCC GGACTCCAGC ACTGGCAATC 
GCATCAGGCC CTTGTGTGTA TGTGTATAAG AATCTCAAGC CCTACTTCAA GTTCAGCCTG 
CCCTCGCTGC CTACAAACCC CCTGGAACAA GACCTTTGGA ATCAGGCCAA AGAGGACCAG 
ATTGACCCCT TGACCCTGAA GGAGATGCTG GAGGGCATCA GGGAGAAGGC AGAGGTGCCT 
TTGTCTGTAC AGTCACTCAG GTTTCTGCCG CTGGAGCTGA GTGAAATGGA GGCGTTTGTG 
AACCAGCACA AATCCAAGTC CATCAGGCGG CAGACAGTCA TCACCACCAT GACCACCTTA 
AAGAAGAACC TGGCTGACGA GGACGCTGTG TCCTGCCTGG TGCTGGGCAC TGAGAACAAG 
GAGCTCCTGG TGCTAGACCC GGAGGCCTTC ACCATCCTGG CCAAGATGAG CCTCCCCAGC 
GTCCCTGCCT TCCTGGAGGC TTCTGGCCAG TTTGATGTGG AGTTCCGTCT TGCCGCTGCC 
TGCCGCAACG GGAGCATCTA TATACTGAGA AGAGACTCCA AGCGTCCCAA GTACTGCATC 
GAGCTGGGCG CCCAGCCTGT GGGGCTTGTC GGTGTACACA AAGTCCTGGT GGTGGGCAGC 
AACCAAGACA GCCTGCACGG CTTCACCTAC AAGGGTAAGA GGCTGTGGAC AGTGCAGATG 
CCTGCAGCCA TCCTGGCCAT GAACCTCCTG GAGCAGCACT CCCGGGGCCT GCAGGCCGTC 
ATGGCTGCGC TGGCCAACGA AGAGGTCCGC ATTTACCATG ACAAGGTCCT ACTCAACGTC 
ATCCGCACGC CGGAGGCAGT GACCAGCCTT TGCTTTGGCC GCTATGGGCG GGAGGATAAT 
ACCCTTATCA TGACTACACT AGGTGGTGGC CTGATCATCA AGATCCTCAA GCGCACAGCG 
GTGTTTGCCG AGGGGGGCGG TGAGGCCGGC CCCCCACCAA GCCAGGCCAT AAAACTCAAC 
GTGCCCCGGA AGACCCGGCT GTACGTGGAC CAGACACTGC GAGAGCGGGA AGCCGGTACC 
GCCATGCACC GGACCTTCCA GGCGGACCTC TACCTGCTGC GCCTCCGGGC GGCCCGTGCC 
TACGTGCAGG CCCTTGAGTC CAGCCTGAGC CCCGTGTCTC TGACGGCCCG GGAGCCGCTC 
AAGCTGCACG CTGTGGTCCA GGGCCTGGGC CCCACCTTCA AGCTCACCCT TCACCTGCAG 
AACACATCAA CAGCCCGCCC CATCCTGGGG CTAGTGGTCT GCTTCCTGTA CAACGAGGTG 
CTCTATGCTC TTCCCCGGGC CTTCTTCAAG GTCCCATTGC TGGTGCCAGG GCTCAACTAC 
CCCCTGGAGA CCTTTGTGAA GAGTCTTAGT GACAAGGGCA TCTCAGACAT CATCAAGGTG 
CTGGTGCTTC GAGAAGGCCA GAGCACACCC CTGCTGAGTG CCCACATCAA CATGCCCATG 
AGTGAGGGGC TGGCAGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005227291.1
CDS	542..2545
Translation	MGRIRDKLYKSERGIASQLVETPARTSATCSGASLSRAGKLSARNLDTRASPQPGSFWALRGGWPQPVARVTPGKMAATSSSDSDGGKGESEANSKWLDSLSDSMANIHTFSACLALADFHGDGEYKLAMGDLGPDGRQPRLKVLKGHTLVSQKPLPDLPAAAVTFLMASHEPRTPALAIASGPCVYVYKNLKPYFKFSLPSLPTNPLEQDLWNQAKEDQIDPLTLKEMLEGIREKAEVPLSVQSLRFLPLELSEMEAFVNQHKSKSIRRQTVITTMTTLKKNLADEDAVSCLVLGTENKELLVLDPEAFTILAKMSLPSVPAFLEASGQFDVEFRLAAACRNGSIYILRRDSKRPKYCIELGAQPVGLVGVHKVLVVGSNQDSLHGFTYKGKRLWTVQMPAAILAMNLLEQHSRGLQAVMAALANEEVRIYHDKVLLNVIRTPEAVTSLCFGRYGREDNTLIMTTLGGGLIIKILKRTAVFAEGGGEAGPPPSQAIKLNVPRKTRLYVDQTLREREAGTAMHRTFQADLYLLRLRAARAYVQALESSLSPVSLTAREPLKLHAVVQGLGPTFKLTLHLQNTSTARPILGLVVCFLYNEVLYALPRAFFKVPLLVPGLNYPLETFVKSLSDKGISDIIKVLVLREGQSTPLLSAHINMPMSEGLAAD

Target ORF information:

RefSeq Version	XM_005227234.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Bardet-Biedl syndrome 1 (BBS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005227234.4

ORF Insert Sequence:

ATGGGAAGAA TCAGAGATAA ATTATATAAG TCGGAACGAG GGATTGCATC TCAGCTGGTG 
GAAACCCCGG CAAGGACCAG CGCAACTTGC TCAGGAGCCT CTCTGTCCCG GGCCGGAAAG 
TTGAGCGCCC GGAACCTTGA TACGCGAGCT TCCCCCCAAC CCGGAAGCTT TTGGGCACTG 
CGCGGCGGCT GGCCACAGCC GGTTGCCAGG GTGACGCCTG GAAAGATGGC TGCTACGTCT 
TCGTCGGATT CAGACGGCGG CAAAGGCGAG AGTGAGGCCA ATTCGAAATG GCTGGACTCG 
CTCAGCGATT CCATGGCGAA CATCCACACC TTTTCCGCCT GCCTGGCGCT GGCCGATTTT 
CACGGGGACG GGGAGTACAA GCTGGCCATG GGAGACCTTG GCCCAGATGG GCGGCAGCCC 
CGCCTGAAGG TGCTTAAAGG ACACACACTG GTGAGCCAGA AACCGCTACC CGACCTGCCA 
GCCGCTGCTG TCACCTTCCT CATGGCGTCA CACGAGCCCC GGACTCCAGC ACTGGCAATC 
GCATCAGGCC CTTGTGTGTA TGTGTATAAG AATCTCAAGC CCTACTTCAA GTTCAGCCTG 
CCCTCGCTGC CTACAAACCC CCTGGAACAA GACCTTTGGA ATCAGGCCAA AGAGGACCAG 
ATTGACCCCT TGACCCTGAA GGAGATGCTG GAGGGCATCA GGGAGAAGGC AGAGGTGCCT 
TTGTCTGTAC AGTCACTCAG GTTTCTGCCG CTGGAGCTGA GTGAAATGGA GGCGTTTGTG 
AACCAGCACA AATCCAAGTC CATCAGGCGG CAGACAGTCA TCACCACCAT GACCACCTTA 
AAGAAGAACC TGGCTGACGA GGACGCTGTG TCCTGCCTGG TGCTGGGCAC TGAGAACAAG 
GAGCTCCTGG TGCTAGACCC GGAGGCCTTC ACCATCCTGG CCAAGATGAG CCTCCCCAGC 
GTCCCTGCCT TCCTGGAGGC TTCTGGCCAG TTTGATGTGG AGTTCCGTCT TGCCGCTGCC 
TGCCGCAACG GGAGCATCTA TATACTGAGA AGAGACTCCA AGCGTCCCAA GTACTGCATC 
GAGCTGGGCG CCCAGCCTGT GGGGCTTGTC GGTGTACACA AAGTCCTGGT GGTGGGCAGC 
AACCAAGACA GCCTGCACGG CTTCACCTAC AAGGGTAAGA GGCTGTGGAC AGTGCAGATG 
CCTGCAGCCA TCCTGGCCAT GAACCTCCTG GAGCAGCACT CCCGGGGCCT GCAGGCCGTC 
ATGGCTGCGC TGGCCAACGA AGAGGTCCGC ATTTACCATG ACAAGGTCCT ACTCAACGTC 
ATCCGCACGC CGGAGGCAGT GACCAGCCTT TGCTTTGGCC GCTATGGGCG GGAGGATAAT 
ACCCTTATCA TGACTACACT AGGTGGTGGC CTGATCATCA AGATCCTCAA GCGCACAGCG 
GTGTTTGCCG AGGGGGGCGG TGAGGCCGGC CCCCCACCAA GCCAGGCCAT AAAACTCAAC 
GTGCCCCGGA AGACCCGGCT GTACGTGGAC CAGACACTGC GAGAGCGGGA AGCCGGTACC 
GCCATGCACC GGACCTTCCA GGCGGACCTC TACCTGCTGC GCCTCCGGGC GGCCCGTGCC 
TACGTGCAGG CCCTTGAGTC CAGCCTGAGC CCCGTGTCTC TGACGGCCCG GGAGCCGCTC 
AAGCTGCACG CTGTGGTCCA GGGCCTGGGC CCCACCTTCA AGCTCACCCT TCACCTGCAG 
AACACATCAA CAGCCCGCCC CATCCTGGGG CTAGTGGTCT GCTTCCTGTA CAACGAGGTG 
CTCTATGCTC TTCCCCGGGC CTTCTTCAAG GTCCCATTGC TGGTGCCAGG GCTCAACTAC 
CCCCTGGAGA CCTTTGTGAA GAGTCTTAGT GACAAGGGCA TCTCAGACAT CATCAAGGTG 
CTGGTGCTTC GAGAAGGCCA GAGCACACCC CTGCTGAGTG CCCACATCAA CATGCCCATG 
AGTGAGGGGC TGGCAGCTGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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BBS1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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