CCDC180 cDNA ORF clone, Bos taurus(cattle)

The following CCDC180 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCDC180 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237463 XM_015472555.2
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237464 XM_015472556.2
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa237460 XM_015472552.2
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237461 XM_024996220.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa237462 XM_015472554.2
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa05854 XM_015472551.2
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa05854 XM_015472549.2
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa05854 XM_015472550.2
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91288 XM_015472550.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91288 XM_015472551.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91288 XM_015464561.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91288 XM_015472549.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91288 XM_010798727.2
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91288 XM_015464562.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91289 XM_015472552.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91289 XM_015464563.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91290 XM_015472553.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91290 XM_015464564.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91291 XM_015464565.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91291 XM_015472554.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa91292 XM_015464566.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa91292 XM_015472555.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa91293 XM_015472556.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa91293 XM_015464567.1
Latest version!
Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237463
    Clone ID Related Accession (Same CDS sequence) XM_015472555.2
    Accession Version XM_015472555.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472555.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AGATGGAGCC CCCCAAACAG AAAACTTCAA TCCTAATACG GAGGAAACTC
    ACCGGGCTGT CCCTGGAGGA AGAGAGTGAG AAGCCCCTGA TTGAACGGGG GAGCAGAAAG
    TGGCCAGGAA TCAAACCTAC TGAAGTCACC ATCCAAAACA AGATTCTCCT CCGGAAAACA
    CCATGTATCA CAACCACCAA AACAACACTG GGCCACCTGG CATCTGTGGA AGCCCGAGAT
    GCTGTCTACC TGAAATATTT GGCATCATTT GAAGAGGAGC TGAAGAAGGT CCAGGAGCAC
    ACCGCACTGC AGGTGAAAGA GGCTCAGCGC TGGAAGGACA GCTGGAAACA CTCCGTGAAC
    ATCATCCAGG GCCTGTACTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328041.2
    CDS362..4825
    Translation

    Target ORF information:

    RefSeq Version XM_015472555.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472555.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AGATGGAGCC CCCCAAACAG AAAACTTCAA TCCTAATACG GAGGAAACTC
    ACCGGGCTGT CCCTGGAGGA AGAGAGTGAG AAGCCCCTGA TTGAACGGGG GAGCAGAAAG
    TGGCCAGGAA TCAAACCTAC TGAAGTCACC ATCCAAAACA AGATTCTCCT CCGGAAAACA
    CCATGTATCA CAACCACCAA AACAACACTG GGCCACCTGG CATCTGTGGA AGCCCGAGAT
    GCTGTCTACC TGAAATATTT GGCATCATTT GAAGAGGAGC TGAAGAAGGT CCAGGAGCAC
    ACCGCACTGC AGGTGAAAGA GGCTCAGCGC TGGAAGGACA GCTGGAAACA CTCCGTGAAC
    ATCATCCAGG GCCTGTACTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237464
    Clone ID Related Accession (Same CDS sequence) XM_015472556.2
    Accession Version XM_015472556.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472556.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG GACCCCTTTT GATGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328042.2
    CDS385..4494
    Translation

    Target ORF information:

    RefSeq Version XM_015472556.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472556.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG GACCCCTTTT GATGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237460
    Clone ID Related Accession (Same CDS sequence) XM_015472552.2
    Accession Version XM_015472552.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG CTAAAAAGCG TGTTGAAGAA ATATGTGGAA
    ATCATAGAGA AAACTTCCTA CCTCATGCAG CCCGACGTGT ATAGGCTGAT CAATAAAGAA
    GCCATGATCA TAAACCACGC CCTGCTGGGC AACCGGAGGG CTCTCGCCAA GCTGTTCGTC
    AACCTGATGG AAGCCAGGCT GCAGCAGGAG CTTGATAGCC ACCGCCGCTG GCAGGGCCTG
    ATGGATGCCT GGAAGGCTCT CAAGAAGGAG TATCTGGTGC AGGATTTCAG TGAGTTCATG
    GCCAGTGAGA GAATCCAGAC TCCTCCGGCT GTGAAGAAAG AGCTTGAGAC CATGATGAAG
    AACCAGAGCA GCCTGCAGCA AAAGCGACTG GACCATCTGT GCACCATCTG TGACCTCCTG
    CCCCCCAATT ACAGCAAAGC TCAGCTAACG GAATGGCGTT CTTCTCTCGA CTCGTTGAAC
    AAGCACATCG ATGCCTACCA CATGGACTGC ATGATGCGGA TCCGCTTGCA GTACGAGAAG
    ATCTGGCAGG AGTGCCTAGC CCAAGTGCAG AAGTGCAGGA AGCAGCTGCT GGACTGGAAG
    GCCTTCACTG AGGAGGAGGC AGAGAGCCTG GTGAGCCCAT CCTTCTTCCA GATGGTGGGA
    TGTCTCCAGA GCAAGGTGGA GGAGGAGCTG GAGGTCTTGG ACAAATCCTT CGAGACCGTG
    GCAAAGCAGA CAGAGCAGCA GAGCTCAGAC CTCTTCAGCT ACTTCCAGGA GGCCGTGAAC
    CTATGGGAGA CACATCAGAG TGTGCTGTTG ATGCAGGAGA TGGAGCTCGA GAAGAGGATG
    GAGCAGCAGC GGCAGAAGCA CAGGCGGGAG AACCAGGCCC AGGAGGCTCA TCTTGACAAG
    CTCTTGGACC AACTGAGGCA GCAAAGCTAT GAGAATAACC TGAAGTCACA CCTGGAAAAG
    GCCAAGGATT TTCTGAAGAA TATGCAATGC AGGTATGAGG CTTTTCATGG CCTCCTGACA
    AAGGAAGTGA TGGAGTACCC AGTGATCATA CTGAAGGAAC TCAACTCCTA CAGCTCCCAC
    CTCAGCCGAC ACTTCTGTGT TCGTGAAATC TTTGAACAGA ACTTGGAGGG AGAAGTCATT
    TTCCAATTGA GGGAACCAGA GCCACATGAA AAACAGTTCC AGAAGAGAAT GAGAAGACCG
    AGAGGAAAAC GAAGGTCCAA AGCAAACGCC CGCAAAGGTG ACGAAAGCAA CAGTGGGAGA
    ACGAGTTCCC TCAAGCCAGC CGAAGAGATA GAAGAAGAAG AAGATCAAGA GGCCGAGTCC
    TCCACCACAC AAGAGGTGGC CGTGAGCCCA CAGGAGAAGT CCGTACTGAG TGAAGAGATG
    GATGAATCCA GGGAGGACTC TATTTCAGGA CTGGAAGAAA TACAGGTTGA AAGAGACAGC
    TCCTTGAGAT CACCCCCAAA CCAGGCCAGT GTGATAGTTC ACGAAGAAGA AGAGGAGGAG
    AAGAAGGAAA AGGTGGAGGA GGAGAGGGAG GAGGAGGAGG AGGAAGAGGA GGAGGAGGAA
    GAGGAGGAGG AGAAGGAGGA AGAGGAGGAG GAGCGGAGGA GCTTGTCTGT GGATGAGGTC
    AAAGCCCAGG AGGAGAGTCT AGGGGAGATC TCCCAGGAGG AGATGGAGTT CTTCACAACC
    TCCAGTGGGA ACACTTACTT TGTTTTCCTA CCCCTGAAAC AAGAAGAGAA CAGCAAGACA
    CCCCACTCCA ACCTTCCTGC CACTCTCTTC AAGGACATTT CCAGTGCCAG GGTCTTAGAA
    GAAGTGATCA TTCCATCCAG ACTAGTTTTA GAGATTAAGA AACAGCTTCG AACTGGCTTT
    TTTGAGCACT TGGAGAAATG GTTTGACCAA TGTGCCCTCA ATGCTCGGGT CATCGTGGCC
    AGCAAGATTG ATGAGCTGGA TTCAGAACTG GAGCTGCGTC TGCACCTGCA CCAGCCAAGA
    GCCCAGCACG TTGAGAAGGA CATCCACAAT GTCAGAGCAG CTGAGCTCTT GCTTCATCAA
    GAACGGCTGA ACAGCCACTG TGCAGGGGTG ATGGAGACAC TGAGGAAGGA GAGGATGATG
    TTCTGCCAGT TCCAAGAAGA GCAAAACTTG AAGAGCAAAC ACTTCCGCCG CAAGATTTCT
    GACATGGAGC ACATCTTCTT GAACGCCACC AAGAGCCAGA AGCTGGCGAC TCTCAGCAGC
    ACTCTGCAGC GGGAGCTGCT CAGCTACGTC GACGTCATCC AGGTGTCCCT GCGCAGCTTC
    CGCCAGTACC TGGAGGAGAG CATGGGCAAG CTGCGCTACA CCAACATTGA GTTCATCAAG
    CACTGCAGAT TGTTTTCAGA GGGAGGCAAC TTCTCTTCTG AAGAAATTGA CTCACTGTGT
    CATCGACTAG AGAAGGAAGC TGCCCGGATA GAGTTTGTTG AAAATTTAAT CATGATCAAC
    ATGGAAAAGA TGGAGAGTGA TTACCTGGAC CAGGCTAATG AGTTCATCAG CAAGTTTGAA
    AGTAAATTCC ATAACCTGTC TGTGGACCTT ATTTTCATAG AGAAAATCCA GCGCTTGCTG
    ACAAATTTGC AAGTAAACCT CAAGTGCGAG GTGGCAAAAT CCAATTTGCA GACAGAGGGA
    TTAAATTCTT CTCTAGAACA GCTTCAAAGC AAGGTAGAAA TGTGCCGAAG CTTAAAAAGG
    AATAAACAAG TAGTGAGCAC AGAAGACCTC CTTGGCCTTA TTCGAACTTG GAAAGAAAAA
    CTGAGCCAAA GGATTGAGTA TCTCAACTGC AAGCTGGACA TGTTATCCAT GACTCACCTG
    GCCTTTCCTG ACAACGTCTT GACTGACCTG GAGGTAGATA GTGACATCTT GTTATCTTCA
    GAAGTCTATG AAGAAGAGGC TAAGGTAGAC ATGGTCACCC CTGAGTCCTT TGCCCAGCCA
    AGCCGCATGG GGAAGTCCAT GATTGAAGAC CCAGCTGTGG AGGTGGTCAA GAGGATCCTG
    CAGTTTCCTG ACTCAAAGTC CTCAACCCTT CAGTGTGACA AAGAACGCTC CCAGACAGCC
    TTGAGGCGAC TCCGGAACCG AGCAGAGCAC TCCTTGAAGA AGGACTTGTC CATCGCCAGT
    GCCACCTCGG TAGCAAGCGT CACGCGGTAC ACCAAGCCCA ACCGACTGGA TAGAAAGTAC
    CAGGTGCTCG GTGACAAGCC TCCTCCAGCA GAGGATTTTA AAGGCATCGT CCTGAGCCTG
    CTCTGGGAGA GCAACGAGCA CCTGCTGGCG GTCGCGGAGG AGTTTTACCG CAAAGAGAAA
    CGCTCAGTCA CCAGGCCCGA CTGCATGTAC GAAACCTTCG ACCAGTGCGC GGAGAACATC
    TGCAAAAAGA TCCTGGAGTA CCACAGCCAG ACAGACGATT ACTACAACTC CTGCCTCATA
    GAATTACGGG CCCAGATGAG GAGATTTGAG GAGCTTCTGC CCCAGGTGTG TTGGCTGGTG
    ATGGAGAATT TCAAGGAGCA CCATTGGAAA AAATGTTGTG CTTCCACCAA AGAGATCAGA
    GAACAGTTCT TGAATTACCA GGAAGAGCTG GAGAAAAGGA AGGATGAAAA TGCCCAGAAG
    CTCCACCCAA ATCTCGGACA CCCTGCACAG CTCCAAGATA TGGAGTCTCT ATGTCAAGTG
    GAAGAGAAGA GGCAGGGAGA TTTGGACGCC TTGATTGTGG TAACCAAGGA GAAGCTGGAG
    GAATGCACAA GGAAGTACGC CCGGTTTTTC ATAGCCACCT TGGCCACCTT CACCGAGAAG
    TTCCTGCTGC AGTTGGATGA GGTGGTCACC ATTGACGATG TCCAGGTTGC AAAGATGGAG
    CCCCCCAAAC AGAAAACTTC AATCCTAATA CGGAGGAAAC TCACCGGGCT GTCCCTGGAG
    GAAGAGAGTG AGAAGCCCCT GATTGAACGG GGGAGCAGAA AGTGGCCAGG AATCAAACCT
    ACTGAAGTCA CCATCCAAAA CAAGATTCTC CTCCGGAAAA CACCATGTAT CACAACCACC
    AAAACAACAC TGGGCCACCT GGCATCTGTG GAAGCCCGAG ATGCTGTCTA CCTGAAATAT
    TTGGCATCAT TTGAAGAGGA GCTGAAGAAG GTCCAGGAGC ACACCGCACT GCAGGTGAAA
    GAGGCTCAGC GCTGGAAGGA CAGCTGGAAA CACTCCGTGA ACATCATCCA GGGCCTGTAC
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328038.2
    CDS357..5222
    Translation

    Target ORF information:

    RefSeq Version XM_015472552.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472552.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG CTAAAAAGCG TGTTGAAGAA ATATGTGGAA
    ATCATAGAGA AAACTTCCTA CCTCATGCAG CCCGACGTGT ATAGGCTGAT CAATAAAGAA
    GCCATGATCA TAAACCACGC CCTGCTGGGC AACCGGAGGG CTCTCGCCAA GCTGTTCGTC
    AACCTGATGG AAGCCAGGCT GCAGCAGGAG CTTGATAGCC ACCGCCGCTG GCAGGGCCTG
    ATGGATGCCT GGAAGGCTCT CAAGAAGGAG TATCTGGTGC AGGATTTCAG TGAGTTCATG
    GCCAGTGAGA GAATCCAGAC TCCTCCGGCT GTGAAGAAAG AGCTTGAGAC CATGATGAAG
    AACCAGAGCA GCCTGCAGCA AAAGCGACTG GACCATCTGT GCACCATCTG TGACCTCCTG
    CCCCCCAATT ACAGCAAAGC TCAGCTAACG GAATGGCGTT CTTCTCTCGA CTCGTTGAAC
    AAGCACATCG ATGCCTACCA CATGGACTGC ATGATGCGGA TCCGCTTGCA GTACGAGAAG
    ATCTGGCAGG AGTGCCTAGC CCAAGTGCAG AAGTGCAGGA AGCAGCTGCT GGACTGGAAG
    GCCTTCACTG AGGAGGAGGC AGAGAGCCTG GTGAGCCCAT CCTTCTTCCA GATGGTGGGA
    TGTCTCCAGA GCAAGGTGGA GGAGGAGCTG GAGGTCTTGG ACAAATCCTT CGAGACCGTG
    GCAAAGCAGA CAGAGCAGCA GAGCTCAGAC CTCTTCAGCT ACTTCCAGGA GGCCGTGAAC
    CTATGGGAGA CACATCAGAG TGTGCTGTTG ATGCAGGAGA TGGAGCTCGA GAAGAGGATG
    GAGCAGCAGC GGCAGAAGCA CAGGCGGGAG AACCAGGCCC AGGAGGCTCA TCTTGACAAG
    CTCTTGGACC AACTGAGGCA GCAAAGCTAT GAGAATAACC TGAAGTCACA CCTGGAAAAG
    GCCAAGGATT TTCTGAAGAA TATGCAATGC AGGTATGAGG CTTTTCATGG CCTCCTGACA
    AAGGAAGTGA TGGAGTACCC AGTGATCATA CTGAAGGAAC TCAACTCCTA CAGCTCCCAC
    CTCAGCCGAC ACTTCTGTGT TCGTGAAATC TTTGAACAGA ACTTGGAGGG AGAAGTCATT
    TTCCAATTGA GGGAACCAGA GCCACATGAA AAACAGTTCC AGAAGAGAAT GAGAAGACCG
    AGAGGAAAAC GAAGGTCCAA AGCAAACGCC CGCAAAGGTG ACGAAAGCAA CAGTGGGAGA
    ACGAGTTCCC TCAAGCCAGC CGAAGAGATA GAAGAAGAAG AAGATCAAGA GGCCGAGTCC
    TCCACCACAC AAGAGGTGGC CGTGAGCCCA CAGGAGAAGT CCGTACTGAG TGAAGAGATG
    GATGAATCCA GGGAGGACTC TATTTCAGGA CTGGAAGAAA TACAGGTTGA AAGAGACAGC
    TCCTTGAGAT CACCCCCAAA CCAGGCCAGT GTGATAGTTC ACGAAGAAGA AGAGGAGGAG
    AAGAAGGAAA AGGTGGAGGA GGAGAGGGAG GAGGAGGAGG AGGAAGAGGA GGAGGAGGAA
    GAGGAGGAGG AGAAGGAGGA AGAGGAGGAG GAGCGGAGGA GCTTGTCTGT GGATGAGGTC
    AAAGCCCAGG AGGAGAGTCT AGGGGAGATC TCCCAGGAGG AGATGGAGTT CTTCACAACC
    TCCAGTGGGA ACACTTACTT TGTTTTCCTA CCCCTGAAAC AAGAAGAGAA CAGCAAGACA
    CCCCACTCCA ACCTTCCTGC CACTCTCTTC AAGGACATTT CCAGTGCCAG GGTCTTAGAA
    GAAGTGATCA TTCCATCCAG ACTAGTTTTA GAGATTAAGA AACAGCTTCG AACTGGCTTT
    TTTGAGCACT TGGAGAAATG GTTTGACCAA TGTGCCCTCA ATGCTCGGGT CATCGTGGCC
    AGCAAGATTG ATGAGCTGGA TTCAGAACTG GAGCTGCGTC TGCACCTGCA CCAGCCAAGA
    GCCCAGCACG TTGAGAAGGA CATCCACAAT GTCAGAGCAG CTGAGCTCTT GCTTCATCAA
    GAACGGCTGA ACAGCCACTG TGCAGGGGTG ATGGAGACAC TGAGGAAGGA GAGGATGATG
    TTCTGCCAGT TCCAAGAAGA GCAAAACTTG AAGAGCAAAC ACTTCCGCCG CAAGATTTCT
    GACATGGAGC ACATCTTCTT GAACGCCACC AAGAGCCAGA AGCTGGCGAC TCTCAGCAGC
    ACTCTGCAGC GGGAGCTGCT CAGCTACGTC GACGTCATCC AGGTGTCCCT GCGCAGCTTC
    CGCCAGTACC TGGAGGAGAG CATGGGCAAG CTGCGCTACA CCAACATTGA GTTCATCAAG
    CACTGCAGAT TGTTTTCAGA GGGAGGCAAC TTCTCTTCTG AAGAAATTGA CTCACTGTGT
    CATCGACTAG AGAAGGAAGC TGCCCGGATA GAGTTTGTTG AAAATTTAAT CATGATCAAC
    ATGGAAAAGA TGGAGAGTGA TTACCTGGAC CAGGCTAATG AGTTCATCAG CAAGTTTGAA
    AGTAAATTCC ATAACCTGTC TGTGGACCTT ATTTTCATAG AGAAAATCCA GCGCTTGCTG
    ACAAATTTGC AAGTAAACCT CAAGTGCGAG GTGGCAAAAT CCAATTTGCA GACAGAGGGA
    TTAAATTCTT CTCTAGAACA GCTTCAAAGC AAGGTAGAAA TGTGCCGAAG CTTAAAAAGG
    AATAAACAAG TAGTGAGCAC AGAAGACCTC CTTGGCCTTA TTCGAACTTG GAAAGAAAAA
    CTGAGCCAAA GGATTGAGTA TCTCAACTGC AAGCTGGACA TGTTATCCAT GACTCACCTG
    GCCTTTCCTG ACAACGTCTT GACTGACCTG GAGGTAGATA GTGACATCTT GTTATCTTCA
    GAAGTCTATG AAGAAGAGGC TAAGGTAGAC ATGGTCACCC CTGAGTCCTT TGCCCAGCCA
    AGCCGCATGG GGAAGTCCAT GATTGAAGAC CCAGCTGTGG AGGTGGTCAA GAGGATCCTG
    CAGTTTCCTG ACTCAAAGTC CTCAACCCTT CAGTGTGACA AAGAACGCTC CCAGACAGCC
    TTGAGGCGAC TCCGGAACCG AGCAGAGCAC TCCTTGAAGA AGGACTTGTC CATCGCCAGT
    GCCACCTCGG TAGCAAGCGT CACGCGGTAC ACCAAGCCCA ACCGACTGGA TAGAAAGTAC
    CAGGTGCTCG GTGACAAGCC TCCTCCAGCA GAGGATTTTA AAGGCATCGT CCTGAGCCTG
    CTCTGGGAGA GCAACGAGCA CCTGCTGGCG GTCGCGGAGG AGTTTTACCG CAAAGAGAAA
    CGCTCAGTCA CCAGGCCCGA CTGCATGTAC GAAACCTTCG ACCAGTGCGC GGAGAACATC
    TGCAAAAAGA TCCTGGAGTA CCACAGCCAG ACAGACGATT ACTACAACTC CTGCCTCATA
    GAATTACGGG CCCAGATGAG GAGATTTGAG GAGCTTCTGC CCCAGGTGTG TTGGCTGGTG
    ATGGAGAATT TCAAGGAGCA CCATTGGAAA AAATGTTGTG CTTCCACCAA AGAGATCAGA
    GAACAGTTCT TGAATTACCA GGAAGAGCTG GAGAAAAGGA AGGATGAAAA TGCCCAGAAG
    CTCCACCCAA ATCTCGGACA CCCTGCACAG CTCCAAGATA TGGAGTCTCT ATGTCAAGTG
    GAAGAGAAGA GGCAGGGAGA TTTGGACGCC TTGATTGTGG TAACCAAGGA GAAGCTGGAG
    GAATGCACAA GGAAGTACGC CCGGTTTTTC ATAGCCACCT TGGCCACCTT CACCGAGAAG
    TTCCTGCTGC AGTTGGATGA GGTGGTCACC ATTGACGATG TCCAGGTTGC AAAGATGGAG
    CCCCCCAAAC AGAAAACTTC AATCCTAATA CGGAGGAAAC TCACCGGGCT GTCCCTGGAG
    GAAGAGAGTG AGAAGCCCCT GATTGAACGG GGGAGCAGAA AGTGGCCAGG AATCAAACCT
    ACTGAAGTCA CCATCCAAAA CAAGATTCTC CTCCGGAAAA CACCATGTAT CACAACCACC
    AAAACAACAC TGGGCCACCT GGCATCTGTG GAAGCCCGAG ATGCTGTCTA CCTGAAATAT
    TTGGCATCAT TTGAAGAGGA GCTGAAGAAG GTCCAGGAGC ACACCGCACT GCAGGTGAAA
    GAGGCTCAGC GCTGGAAGGA CAGCTGGAAA CACTCCGTGA ACATCATCCA GGGCCTGTAC
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237461
    Clone ID Related Accession (Same CDS sequence) XM_024996220.1
    Accession Version XM_024996220.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATCCCAA AGGACAGCAG GAAGCCCTTG GTGAAGAAGG TGGATGTCTG TGAAGGCGAG 
    ATGATGTCTC CTCGGCAGCG GAAGTGGATG CGCAGCCTAC CCAATGACTG GACCACAGAA
    AACCCTGTTC TTTACAGGGA AAAGCAAATA GCCAAAAAGG AAAAAGCTCA AGAGAGTGAG
    AACGCCATCG ACGCCAGAGA AGTCCGGGGC CTCACGGACA CCATTGTTCC TGAGAGGATC
    AGCAGCACCA CCTTTCAAAA GCAAGGAGAT TCCAAGAGGA GGGGCTACGA GAGTGCCCTG
    GCGAGCTTTA AGGAGGAGAT CGCTCAGATT GGGATGGAAA TGGAACCTCT CATCTTGGAA
    CCAGGAGCAC TTCTTTTAAA AAAGCTGGCT GAGTCTGATG AGGATGTGAA CAATCTCTTC
    AAAAAGGTGG AAAACGACAG CAACCTCGAA GACTACACCA TCCAAACCCT GTTGGAGCTG
    AGGGATCAGG TGGCCGAGAA GTTCCTGCTG CAGAAGCAGG AGATCAAGGA GCTGGATGAG
    ACACTGCACT CACTTGAGTT CTCCCGGGTA GATAAACTAA AAAGCGTGTT GAAGAAATAT
    GTGGAAATCA TAGAGAAAAC TTCCTACCTC ATGCAGCCCG ACGTGTATAG GCTGATCAAT
    AAAGAAGCCA TGATCATAAA CCACGCCCTG CTGGGCAACC GGAGGGCTCT CGCCAAGCTG
    TTCGTCAACC TGATGGAAGC CAGGCTGCAG CAGGAGCTTG ATAGCCACCG CCGCTGGCAG
    GGCCTGATGG ATGCCTGGAA GGCTCTCAAG AAGGAGTATC TGGTGCAGGA TTTCAGTGAG
    TTCATGGCCA GTGAGAGAAT CCAGACTCCT CCGGCTGTGA AGAAAGAGCT TGAGACCATG
    ATGAAGAACC AGAGCAGCCT GCAGCAAAAG CGACTGGACC ATCTGTGCAC CATCTGTGAC
    CTCCTGCCCC CCAATTACAG CAAAGCTCAG CTAACGGAAT GGCGTTCTTC TCTCGACTCG
    TTGAACAAGC ACATCGATGC CTACCACATG GACTGCATGA TGCGGATCCG CTTGCAGTAC
    GAGAAGATCT GGCAGGAGTG CCTAGCCCAA GTGCAGAAGT GCAGGAAGCA GCTGCTGGAC
    TGGAAGGCCT TCACTGAGGA GGAGGCAGAG AGCCTGGTGA GCCCATCCTT CTTCCAGATG
    GTGGGATGTC TCCAGAGCAA GGTGGAGGAG GAGCTGGAGG TCTTGGACAA ATCCTTCGAG
    ACCGTGGCAA AGCAGACAGA GCAGCAGAGC TCAGACCTCT TCAGCTACTT CCAGGAGGCC
    GTGAACCTAT GGGAGACACA TCAGAGTGTG CTGTTGATGC AGGAGATGGA GCTCGAGAAG
    AGGATGGAGC AGCAGCGGCA GAAGCACAGG CGGGAGAACC AGGCCCAGGA GGCTCATCTT
    GACAAGCTCT TGGACCAACT GAGGCAGCAA AGCTATGAGA ATAACCTGAA GTCACACCTG
    GAAAAGGCCA AGGATTTTCT GAAGAATATG CAATGCAGGT ATGAGGCTTT TCATGGCCTC
    CTGACAAAGG AAGTGATGGA GTACCCAGTG ATCATACTGA AGGAACTCAA CTCCTACAGC
    TCCCACCTCA GCCGACACTT CTGTGTTCGT GAAATCTTTG AACAGAACTT GGAGGGAGAA
    GTCATTTTCC AATTGAGGGA ACCAGAGCCA CATGAAAAAC AGTTCCAGAA GAGAATGAGA
    AGACCGAGAG GAAAACGAAG GTCCAAAGCA AACGCCCGCA AAGGTGACGA AAGCAACAGT
    GGGAGAACGA GTTCCCTCAA GCCAGCCGAA GAGATAGAAG AAGAAGAAGA TCAAGAGGCC
    GAGTCCTCCA CCACACAAGA GGTGGCCGTG AGCCCACAGG AGAAGTCCGT ACTGAGTGAA
    GAGATGGATG AATCCAGGGA GGACTCTATT TCAGGACTGG AAGAAATACA GGTTGAAAGA
    GACAGCTCCT TGAGATCACC CCCAAACCAG GCCAGTGTGA TAGTTCACGA AGAAGAAGAG
    GAGGAGAAGA AGGAAAAGGT GGAGGAGGAG AGGGAGGAGG AGGAGGAGGA AGAGGAGGAG
    GAGGAAGAGG AGGAGGAGAA GGAGGAAGAG GAGGAGGAGC GGAGGAGCTT GTCTGTGGAT
    GAGGTCAAAG CCCAGGAGGA GAGTCTAGGG GAGATCTCCC AGGAGGAGAT GGAGTTCTTC
    ACAACCTCCA GTGGGAACAC TTACTTTGTT TTCCTACCCC TGAAACAAGA AGAGAACAGC
    AAGACACCCC ACTCCAACCT TCCTGCCACT CTCTTCAAGG ACATTTCCAG TGCCAGGGTC
    TTAGAAGAAG TGATCATTCC ATCCAGACTA GTTTTAGAGA TTAAGAAACA GCTTCGAACT
    GGCTTTTTTG AGCACTTGGA GAAATGGTTT GACCAATGTG CCCTCAATGC TCGGGTCATC
    GTGGCCAGCA AGATTGATGA GCTGGATTCA GAACTGGAGC TGCGTCTGCA CCTGCACCAG
    CCAAGAGCCC AGCACGTTGA GAAGGACATC CACAATGTCA GAGCAGCTGA GCTCTTGCTT
    CATCAAGAAC GGCTGAACAG CCACTGTGCA GGGGTGATGG AGACACTGAG GAAGGAGAGG
    ATGATGTTCT GCCAGTTCCA AGAAGAGCAA AACTTGAAGA GCAAACACTT CCGCCGCAAG
    ATTTCTGACA TGGAGCACAT CTTCTTGAAC GCCACCAAGA GCCAGAAGCT GGCGACTCTC
    AGCAGCACTC TGCAGCGGGA GCTGCTCAGC TACGTCGACG TCATCCAGGT GTCCCTGCGC
    AGCTTCCGCC AGTACCTGGA GGAGAGCATG GGCAAGCTGC GCTACACCAA CATTGAGTTC
    ATCAAGCACT GCAGATTGTT TTCAGAGGGA GGCAACTTCT CTTCTGAAGA AATTGACTCA
    CTGTGTCATC GACTAGAGAA GGAAGCTGCC CGGATAGAGT TTGTTGAAAA TTTAATCATG
    ATCAACATGG AAAAGATGGA GAGTGATTAC CTGGACCAGG CTAATGAGTT CATCAGCAAG
    TTTGAAAGTA AATTCCATAA CCTGTCTGTG GACCTTATTT TCATAGAGAA AATCCAGCGC
    TTGCTGACAA ATTTGCAAGT AAACCTCAAG TGCGAGGTGG CAAAATCCAA TTTGCAGACA
    GAGGGATTAA ATTCTTCTCT AGAACAGCTT CAAAGCAAGG TAGAAATGTG CCGAAGCTTA
    AAAAGGAATA AACAAGTAGT GAGCACAGAA GACCTCCTTG GCCTTATTCG AACTTGGAAA
    GAAAAACTGA GCCAAAGGAT TGAGTATCTC AACTGCAAGC TGGACATGTT ATCCATGACT
    CACCTGGCCT TTCCTGACAA CGTCTTGACT GACCTGGAGG TAGATAGTGA CATCTTGTTA
    TCTTCAGAAG TCTATGAAGA AGAGGCTAAG GTAGACATGG TCACCCCTGA GTCCTTTGCC
    CAGCCAAGCC GCATGGGGAA GTCCATGATT GAAGACCCAG CTGTGGAGGT GGTCAAGAGG
    ATCCTGCAGT TTCCTGACTC AAAGTCCTCA ACCCTTCAGT GTGACAAAGA ACGCTCCCAG
    ACAGCCTTGA GGCGACTCCG GAACCGAGCA GAGCACTCCT TGAAGAAGGA CTTGTCCATC
    GCCAGTGCCA CCTCGGTAGC AAGCGTCACG CGGTACACCA AGCCCAACCG ACTGGATAGA
    AAGTACCAGG TGCTCGGTGA CAAGCCTCCT CCAGCAGAGG ATTTTAAAGG CATCGTCCTG
    AGCCTGCTCT GGGAGAGCAA CGAGCACCTG CTGGCGGTCG CGGAGGAGTT TTACCGCAAA
    GAGAAACGCT CAGTCACCAG GCCCGACTGC ATGTACGAAA CCTTCGACCA GTGCGCGGAG
    AACATCTGCA AAAAGATCCT GGAGTACCAC AGCCAGACAG ACGATTACTA CAACTCCTGC
    CTCATAGAAT TACGGGCCCA GATGAGGAGA TTTGAGGAGC TTCTGCCCCA GGTGTGTTGG
    CTGGTGATGG AGAATTTCAA GGAGCACCAT TGGAAAAAAT GTTGTGCTTC CACCAAAGAG
    ATCAGAGAAC AGTTCTTGAA TTACCAGGAA GAGCTGGAGA AAAGGAAGGA TGAAAATGCC
    CAGAAGCTCC ACCCAAATCT CGGACACCCT GCACAGCTCC AAGATATGGA GTCTCTATGT
    CAAGTGGAAG AGAAGAGGCA GGGAGATTTG GACGCCTTGA TTGTGGTAAC CAAGGAGAAG
    CTGGAGGAAT GCACAAGGAA GTACGCCCGG TTTTTCATAG CCACCTTGGC CACCTTCACC
    GAGAAGTTCC TGCTGCAGTT GGATGAGGTG GTCACCATTG ACGATGTCCA GGTTGCAAAG
    ATGGAGCCCC CCAAACAGAA AACTTCAATC CTAATACGGA GGAAACTCAC CGGGCTGTCC
    CTGGAGGAAG AGAGTGAGAA GCCCCTGATT GAACGGGGGA GCAGAAAGTG GCCAGGAATC
    AAACCTACTG AAGTCACCAT CCAAAACAAG ATTCTCCTCC GGAAAACACC ATGTATCACA
    ACCACCAAAA CAACACTGGG CCACCTGGCA TCTGTGGAAG CCCGAGATGC TGTCTACCTG
    AAATATTTGG CATCATTTGA AGAGGAGCTG AAGAAGGTCC AGGAGCACAC CGCACTGCAG
    GTGAAAGAGG CTCAGCGCTG GAAGGACAGC TGGAAACACT CCGTGAACAT CATCCAGGGC
    CTGTACTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851988.1
    CDS103..4854
    Translation

    Target ORF information:

    RefSeq Version XM_024996220.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996220.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATCCCAA AGGACAGCAG GAAGCCCTTG GTGAAGAAGG TGGATGTCTG TGAAGGCGAG 
    ATGATGTCTC CTCGGCAGCG GAAGTGGATG CGCAGCCTAC CCAATGACTG GACCACAGAA
    AACCCTGTTC TTTACAGGGA AAAGCAAATA GCCAAAAAGG AAAAAGCTCA AGAGAGTGAG
    AACGCCATCG ACGCCAGAGA AGTCCGGGGC CTCACGGACA CCATTGTTCC TGAGAGGATC
    AGCAGCACCA CCTTTCAAAA GCAAGGAGAT TCCAAGAGGA GGGGCTACGA GAGTGCCCTG
    GCGAGCTTTA AGGAGGAGAT CGCTCAGATT GGGATGGAAA TGGAACCTCT CATCTTGGAA
    CCAGGAGCAC TTCTTTTAAA AAAGCTGGCT GAGTCTGATG AGGATGTGAA CAATCTCTTC
    AAAAAGGTGG AAAACGACAG CAACCTCGAA GACTACACCA TCCAAACCCT GTTGGAGCTG
    AGGGATCAGG TGGCCGAGAA GTTCCTGCTG CAGAAGCAGG AGATCAAGGA GCTGGATGAG
    ACACTGCACT CACTTGAGTT CTCCCGGGTA GATAAACTAA AAAGCGTGTT GAAGAAATAT
    GTGGAAATCA TAGAGAAAAC TTCCTACCTC ATGCAGCCCG ACGTGTATAG GCTGATCAAT
    AAAGAAGCCA TGATCATAAA CCACGCCCTG CTGGGCAACC GGAGGGCTCT CGCCAAGCTG
    TTCGTCAACC TGATGGAAGC CAGGCTGCAG CAGGAGCTTG ATAGCCACCG CCGCTGGCAG
    GGCCTGATGG ATGCCTGGAA GGCTCTCAAG AAGGAGTATC TGGTGCAGGA TTTCAGTGAG
    TTCATGGCCA GTGAGAGAAT CCAGACTCCT CCGGCTGTGA AGAAAGAGCT TGAGACCATG
    ATGAAGAACC AGAGCAGCCT GCAGCAAAAG CGACTGGACC ATCTGTGCAC CATCTGTGAC
    CTCCTGCCCC CCAATTACAG CAAAGCTCAG CTAACGGAAT GGCGTTCTTC TCTCGACTCG
    TTGAACAAGC ACATCGATGC CTACCACATG GACTGCATGA TGCGGATCCG CTTGCAGTAC
    GAGAAGATCT GGCAGGAGTG CCTAGCCCAA GTGCAGAAGT GCAGGAAGCA GCTGCTGGAC
    TGGAAGGCCT TCACTGAGGA GGAGGCAGAG AGCCTGGTGA GCCCATCCTT CTTCCAGATG
    GTGGGATGTC TCCAGAGCAA GGTGGAGGAG GAGCTGGAGG TCTTGGACAA ATCCTTCGAG
    ACCGTGGCAA AGCAGACAGA GCAGCAGAGC TCAGACCTCT TCAGCTACTT CCAGGAGGCC
    GTGAACCTAT GGGAGACACA TCAGAGTGTG CTGTTGATGC AGGAGATGGA GCTCGAGAAG
    AGGATGGAGC AGCAGCGGCA GAAGCACAGG CGGGAGAACC AGGCCCAGGA GGCTCATCTT
    GACAAGCTCT TGGACCAACT GAGGCAGCAA AGCTATGAGA ATAACCTGAA GTCACACCTG
    GAAAAGGCCA AGGATTTTCT GAAGAATATG CAATGCAGGT ATGAGGCTTT TCATGGCCTC
    CTGACAAAGG AAGTGATGGA GTACCCAGTG ATCATACTGA AGGAACTCAA CTCCTACAGC
    TCCCACCTCA GCCGACACTT CTGTGTTCGT GAAATCTTTG AACAGAACTT GGAGGGAGAA
    GTCATTTTCC AATTGAGGGA ACCAGAGCCA CATGAAAAAC AGTTCCAGAA GAGAATGAGA
    AGACCGAGAG GAAAACGAAG GTCCAAAGCA AACGCCCGCA AAGGTGACGA AAGCAACAGT
    GGGAGAACGA GTTCCCTCAA GCCAGCCGAA GAGATAGAAG AAGAAGAAGA TCAAGAGGCC
    GAGTCCTCCA CCACACAAGA GGTGGCCGTG AGCCCACAGG AGAAGTCCGT ACTGAGTGAA
    GAGATGGATG AATCCAGGGA GGACTCTATT TCAGGACTGG AAGAAATACA GGTTGAAAGA
    GACAGCTCCT TGAGATCACC CCCAAACCAG GCCAGTGTGA TAGTTCACGA AGAAGAAGAG
    GAGGAGAAGA AGGAAAAGGT GGAGGAGGAG AGGGAGGAGG AGGAGGAGGA AGAGGAGGAG
    GAGGAAGAGG AGGAGGAGAA GGAGGAAGAG GAGGAGGAGC GGAGGAGCTT GTCTGTGGAT
    GAGGTCAAAG CCCAGGAGGA GAGTCTAGGG GAGATCTCCC AGGAGGAGAT GGAGTTCTTC
    ACAACCTCCA GTGGGAACAC TTACTTTGTT TTCCTACCCC TGAAACAAGA AGAGAACAGC
    AAGACACCCC ACTCCAACCT TCCTGCCACT CTCTTCAAGG ACATTTCCAG TGCCAGGGTC
    TTAGAAGAAG TGATCATTCC ATCCAGACTA GTTTTAGAGA TTAAGAAACA GCTTCGAACT
    GGCTTTTTTG AGCACTTGGA GAAATGGTTT GACCAATGTG CCCTCAATGC TCGGGTCATC
    GTGGCCAGCA AGATTGATGA GCTGGATTCA GAACTGGAGC TGCGTCTGCA CCTGCACCAG
    CCAAGAGCCC AGCACGTTGA GAAGGACATC CACAATGTCA GAGCAGCTGA GCTCTTGCTT
    CATCAAGAAC GGCTGAACAG CCACTGTGCA GGGGTGATGG AGACACTGAG GAAGGAGAGG
    ATGATGTTCT GCCAGTTCCA AGAAGAGCAA AACTTGAAGA GCAAACACTT CCGCCGCAAG
    ATTTCTGACA TGGAGCACAT CTTCTTGAAC GCCACCAAGA GCCAGAAGCT GGCGACTCTC
    AGCAGCACTC TGCAGCGGGA GCTGCTCAGC TACGTCGACG TCATCCAGGT GTCCCTGCGC
    AGCTTCCGCC AGTACCTGGA GGAGAGCATG GGCAAGCTGC GCTACACCAA CATTGAGTTC
    ATCAAGCACT GCAGATTGTT TTCAGAGGGA GGCAACTTCT CTTCTGAAGA AATTGACTCA
    CTGTGTCATC GACTAGAGAA GGAAGCTGCC CGGATAGAGT TTGTTGAAAA TTTAATCATG
    ATCAACATGG AAAAGATGGA GAGTGATTAC CTGGACCAGG CTAATGAGTT CATCAGCAAG
    TTTGAAAGTA AATTCCATAA CCTGTCTGTG GACCTTATTT TCATAGAGAA AATCCAGCGC
    TTGCTGACAA ATTTGCAAGT AAACCTCAAG TGCGAGGTGG CAAAATCCAA TTTGCAGACA
    GAGGGATTAA ATTCTTCTCT AGAACAGCTT CAAAGCAAGG TAGAAATGTG CCGAAGCTTA
    AAAAGGAATA AACAAGTAGT GAGCACAGAA GACCTCCTTG GCCTTATTCG AACTTGGAAA
    GAAAAACTGA GCCAAAGGAT TGAGTATCTC AACTGCAAGC TGGACATGTT ATCCATGACT
    CACCTGGCCT TTCCTGACAA CGTCTTGACT GACCTGGAGG TAGATAGTGA CATCTTGTTA
    TCTTCAGAAG TCTATGAAGA AGAGGCTAAG GTAGACATGG TCACCCCTGA GTCCTTTGCC
    CAGCCAAGCC GCATGGGGAA GTCCATGATT GAAGACCCAG CTGTGGAGGT GGTCAAGAGG
    ATCCTGCAGT TTCCTGACTC AAAGTCCTCA ACCCTTCAGT GTGACAAAGA ACGCTCCCAG
    ACAGCCTTGA GGCGACTCCG GAACCGAGCA GAGCACTCCT TGAAGAAGGA CTTGTCCATC
    GCCAGTGCCA CCTCGGTAGC AAGCGTCACG CGGTACACCA AGCCCAACCG ACTGGATAGA
    AAGTACCAGG TGCTCGGTGA CAAGCCTCCT CCAGCAGAGG ATTTTAAAGG CATCGTCCTG
    AGCCTGCTCT GGGAGAGCAA CGAGCACCTG CTGGCGGTCG CGGAGGAGTT TTACCGCAAA
    GAGAAACGCT CAGTCACCAG GCCCGACTGC ATGTACGAAA CCTTCGACCA GTGCGCGGAG
    AACATCTGCA AAAAGATCCT GGAGTACCAC AGCCAGACAG ACGATTACTA CAACTCCTGC
    CTCATAGAAT TACGGGCCCA GATGAGGAGA TTTGAGGAGC TTCTGCCCCA GGTGTGTTGG
    CTGGTGATGG AGAATTTCAA GGAGCACCAT TGGAAAAAAT GTTGTGCTTC CACCAAAGAG
    ATCAGAGAAC AGTTCTTGAA TTACCAGGAA GAGCTGGAGA AAAGGAAGGA TGAAAATGCC
    CAGAAGCTCC ACCCAAATCT CGGACACCCT GCACAGCTCC AAGATATGGA GTCTCTATGT
    CAAGTGGAAG AGAAGAGGCA GGGAGATTTG GACGCCTTGA TTGTGGTAAC CAAGGAGAAG
    CTGGAGGAAT GCACAAGGAA GTACGCCCGG TTTTTCATAG CCACCTTGGC CACCTTCACC
    GAGAAGTTCC TGCTGCAGTT GGATGAGGTG GTCACCATTG ACGATGTCCA GGTTGCAAAG
    ATGGAGCCCC CCAAACAGAA AACTTCAATC CTAATACGGA GGAAACTCAC CGGGCTGTCC
    CTGGAGGAAG AGAGTGAGAA GCCCCTGATT GAACGGGGGA GCAGAAAGTG GCCAGGAATC
    AAACCTACTG AAGTCACCAT CCAAAACAAG ATTCTCCTCC GGAAAACACC ATGTATCACA
    ACCACCAAAA CAACACTGGG CCACCTGGCA TCTGTGGAAG CCCGAGATGC TGTCTACCTG
    AAATATTTGG CATCATTTGA AGAGGAGCTG AAGAAGGTCC AGGAGCACAC CGCACTGCAG
    GTGAAAGAGG CTCAGCGCTG GAAGGACAGC TGGAAACACT CCGTGAACAT CATCCAGGGC
    CTGTACTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237462
    Clone ID Related Accession (Same CDS sequence) XM_015472554.2
    Accession Version XM_015472554.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC ACGTCACGCG GTACACCAAG CCCAACCGAC TGGATAGAAA GTACCAGGTG
    CTCGGTGACA AGCCTCCTCC AGCAGAGGAT TTTAAAGGCA TCGTCCTGAG CCTGCTCTGG
    GAGAGCAACG AGCACCTGCT GGCGGTCGCG GAGGAGTTTT ACCGCAAAGA GAAACGCTCA
    GTCACCAGGC CCGACTGCAT GTACGAAACC TTCGACCAGT GCGCGGAGAA CATCTGCAAA
    AAGATCCTGG AGTACCACAG CCAGACAGAC GATTACTACA ACTCCTGCCT CATAGAATTA
    CGGGCCCAGA TGAGGAGATT TGAGGAGCTT CTGCCCCAGG TGTGTTGGCT GGTGATGGAG
    AATTTCAAGG AGCACCATTG GAAAAAATGT TGTGCTTCCA CCAAAGAGAT CAGAGAACAG
    TTCTTGAATT ACCAGGAAGA GCTGGAGAAA AGGAAGGATG AAAATGCCCA GAAGCTCCAC
    CCAAATCTCG GACACCCTGC ACAGCTCCAA GATATGGAGT CTCTATGTCA AGTGGAAGAG
    AAGAGGCAGG GAGATTTGGA CGCCTTGATT GTGGTAACCA AGGAGAAGCT GGAGGAATGC
    ACAAGGAAGT ACGCCCGGTT TTTCATAGCC ACCTTGGCCA CCTTCACCGA GAAGTTCCTG
    CTGCAGTTGG ATGAGGTGGT CACCATTGAC GATGTCCAGG TTGCAAAGAT GGAGCCCCCC
    AAACAGAAAA CTTCAATCCT AATACGGAGG AAACTCACCG GGCTGTCCCT GGAGGAAGAG
    AGTGAGAAGC CCCTGATTGA ACGGGGGAGC AGAAAGTGGC CAGGAATCAA ACCTACTGAA
    GTCACCATCC AAAACAAGAT TCTCCTCCGG AAAACACCAT GTATCACAAC CACCAAAACA
    ACACTGGGCC ACCTGGCATC TGTGGAAGCC CGAGATGCTG TCTACCTGAA ATATTTGGCA
    TCATTTGAAG AGGAGCTGAA GAAGGTCCAG GAGCACACCG CACTGCAGGT GAAAGAGGCT
    CAGCGCTGGA AGGACAGCTG GAAACACTCC GTGAACATCA TCCAGGGCCT GTACTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328040.2
    CDS354..5093
    Translation

    Target ORF information:

    RefSeq Version XM_015472554.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472554.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC ACGTCACGCG GTACACCAAG CCCAACCGAC TGGATAGAAA GTACCAGGTG
    CTCGGTGACA AGCCTCCTCC AGCAGAGGAT TTTAAAGGCA TCGTCCTGAG CCTGCTCTGG
    GAGAGCAACG AGCACCTGCT GGCGGTCGCG GAGGAGTTTT ACCGCAAAGA GAAACGCTCA
    GTCACCAGGC CCGACTGCAT GTACGAAACC TTCGACCAGT GCGCGGAGAA CATCTGCAAA
    AAGATCCTGG AGTACCACAG CCAGACAGAC GATTACTACA ACTCCTGCCT CATAGAATTA
    CGGGCCCAGA TGAGGAGATT TGAGGAGCTT CTGCCCCAGG TGTGTTGGCT GGTGATGGAG
    AATTTCAAGG AGCACCATTG GAAAAAATGT TGTGCTTCCA CCAAAGAGAT CAGAGAACAG
    TTCTTGAATT ACCAGGAAGA GCTGGAGAAA AGGAAGGATG AAAATGCCCA GAAGCTCCAC
    CCAAATCTCG GACACCCTGC ACAGCTCCAA GATATGGAGT CTCTATGTCA AGTGGAAGAG
    AAGAGGCAGG GAGATTTGGA CGCCTTGATT GTGGTAACCA AGGAGAAGCT GGAGGAATGC
    ACAAGGAAGT ACGCCCGGTT TTTCATAGCC ACCTTGGCCA CCTTCACCGA GAAGTTCCTG
    CTGCAGTTGG ATGAGGTGGT CACCATTGAC GATGTCCAGG TTGCAAAGAT GGAGCCCCCC
    AAACAGAAAA CTTCAATCCT AATACGGAGG AAACTCACCG GGCTGTCCCT GGAGGAAGAG
    AGTGAGAAGC CCCTGATTGA ACGGGGGAGC AGAAAGTGGC CAGGAATCAA ACCTACTGAA
    GTCACCATCC AAAACAAGAT TCTCCTCCGG AAAACACCAT GTATCACAAC CACCAAAACA
    ACACTGGGCC ACCTGGCATC TGTGGAAGCC CGAGATGCTG TCTACCTGAA ATATTTGGCA
    TCATTTGAAG AGGAGCTGAA GAAGGTCCAG GAGCACACCG CACTGCAGGT GAAAGAGGCT
    CAGCGCTGGA AGGACAGCTG GAAACACTCC GTGAACATCA TCCAGGGCCT GTACTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05854
    Clone ID Related Accession (Same CDS sequence) XM_015472549.2 , XM_015472550.2 , XM_015472551.2
    Accession Version XM_015472549.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328035.2
    CDS355..5229
    Translation

    Target ORF information:

    RefSeq Version XM_015472549.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472549.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05854
    Clone ID Related Accession (Same CDS sequence) XM_015472549.2 , XM_015472550.2 , XM_015472551.2
    Accession Version XM_015472550.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328036.2
    CDS342..5216
    Translation

    Target ORF information:

    RefSeq Version XM_015472550.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472550.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05854
    Clone ID Related Accession (Same CDS sequence) XM_015472549.2 , XM_015472550.2 , XM_015472551.2
    Accession Version XM_015472551.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328037.2
    CDS279..5153
    Translation

    Target ORF information:

    RefSeq Version XM_015472551.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472551.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGA GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91288
    Clone ID Related Accession (Same CDS sequence) XM_015472551.1 , XM_015464562.1 , XM_015472550.1 , XM_015472549.1 , XM_010798727.2 , XM_015464561.1
    Accession Version XM_015472551.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328037.1
    CDS209..5083
    Translation

    Target ORF information:

    RefSeq Version XM_015472551.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91288
    Clone ID Related Accession (Same CDS sequence) XM_015472551.1 , XM_015464562.1 , XM_015472550.1 , XM_015472549.1 , XM_010798727.2 , XM_015464561.1
    Accession Version XM_015464562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320048.1
    CDS209..5083
    Translation

    Target ORF information:

    RefSeq Version XM_015464562.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91288
    Clone ID Related Accession (Same CDS sequence) XM_015472551.1 , XM_015464562.1 , XM_015472550.1 , XM_015472549.1 , XM_010798727.2 , XM_015464561.1
    Accession Version XM_015472550.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328036.1
    CDS272..5146
    Translation

    Target ORF information:

    RefSeq Version XM_015472550.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472550.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91288
    Clone ID Related Accession (Same CDS sequence) XM_015472551.1 , XM_015464562.1 , XM_015472550.1 , XM_015472549.1 , XM_010798727.2 , XM_015464561.1
    Accession Version XM_015472549.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328035.1
    CDS285..5159
    Translation

    Target ORF information:

    RefSeq Version XM_015472549.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472549.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91288
    Clone ID Related Accession (Same CDS sequence) XM_015472551.1 , XM_015464562.1 , XM_015472550.1 , XM_015472549.1 , XM_010798727.2 , XM_015464561.1
    Accession Version XM_010798727.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010797029.2
    CDS285..5159
    Translation

    Target ORF information:

    RefSeq Version XM_010798727.2
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798727.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91288
    Clone ID Related Accession (Same CDS sequence) XM_015472551.1 , XM_015464562.1 , XM_015472550.1 , XM_015472549.1 , XM_010798727.2 , XM_015464561.1
    Accession Version XM_015464561.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320047.1
    CDS272..5146
    Translation

    Target ORF information:

    RefSeq Version XM_015464561.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AATTACGGGC CCAGATGAGG AGATTTGAGG AGCTTCTGCC CCAGGTGTGT
    TGGCTGGTGA TGGAGAATTT CAAGGAGCAC CATTGGAAAA AATGTTGTGC TTCCACCAAA
    GAGATCAGAG AACAGTTCTT GAATTACCAG GAAGAGCTGG AGAAAAGGAA GGATGAAAAT
    GCCCAGAAGC TCCACCCAAA TCTCGGACAC CCTGCACAGC TCCAAGATAT GGAGTCTCTA
    TGTCAAGTGG AAGAGAAGAG GCAGGGAGAT TTGGACGCCT TGATTGTGGT AACCAAGGAG
    AAGCTGGAGG AATGCACAAG GAAGTACGCC CGGTTTTTCA TAGCCACCTT GGCCACCTTC
    ACCGAGAAGT TCCTGCTGCA GTTGGATGAG GTGGTCACCA TTGACGATGT CCAGGTTGCA
    AAGATGGAGC CCCCCAAACA GAAAACTTCA ATCCTAATAC GGAGGAAACT CACCGGGCTG
    TCCCTGGAGG AAGAGAGTGA GAAGCCCCTG ATTGAACGGG GGAGCAGAAA GTGGCCAGGA
    ATCAAACCTA CTGAAGTCAC CATCCAAAAC AAGATTCTCC TCCGGAAAAC ACCATGTATC
    ACAACCACCA AAACAACACT GGGCCACCTG GCATCTGTGG AAGCCCGAGA TGCTGTCTAC
    CTGAAATATT TGGCATCATT TGAAGAGGAG CTGAAGAAGG TCCAGGAGCA CACCGCACTG
    CAGGTGAAAG AGGCTCAGCG CTGGAAGGAC AGCTGGAAAC ACTCCGTGAA CATCATCCAG
    GGCCTGTACT TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91289
    Clone ID Related Accession (Same CDS sequence) XM_015472552.1 , XM_015464563.1
    Accession Version XM_015472552.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG CTAAAAAGCG TGTTGAAGAA ATATGTGGAA
    ATCATAGAGA AAACTTCCTA CCTCATGCAG CCCGACGTGT ATAGGCTGAT CAATAAAGAA
    GCCATGATCA TAAACCACGC CCTGCTGGGC AACCGGAGGG CTCTCGCCAA GCTGTTCGTC
    AACCTGATGG AAGCCAGGCT GCAGCAGGAG CTTGATAGCC ACCGCCGCTG GCAGGGCCTG
    ATGGATGCCT GGAAGGCTCT CAAGAAGGAG TATCTGGTGC AGGATTTCAG TGAGTTCATG
    GCCAGTGAGA GAATCCAGAC TCCTCCGGCT GTGAAGAAAG AGCTTGAGAC CATGATGAAG
    AACCAGAGCA GCCTGCAGCA AAAGCGACTG GACCATCTGT GCACCATCTG TGACCTCCTG
    CCCCCCAATT ACAGCAAAGC TCAGCTAACG GAATGGCGTT CTTCTCTCGA CTCGTTGAAC
    AAGCACATCG ATGCCTACCA CATGGACTGC ATGATGCGGA TCCGCTTGCA GTACGAGAAG
    ATCTGGCAGG AGTGCCTAGC CCAAGTGCAG AAGTGCAGGA AGCAGCTGCT GGACTGGAAG
    GCCTTCACTG AGGAGGAGGC AGAGAGCCTG GTGAGCCCAT CCTTCTTCCA GATGGTGGGA
    TGTCTCCAGA GCAAGGTGGA GGAGGAGCTG GAGGTCTTGG ACAAATCCTT CGAGACCGTG
    GCAAAGCAGA CAGAGCAGCA GAGCTCAGAC CTCTTCAGCT ACTTCCAGGA GGCCGTGAAC
    CTATGGGAGA CACATCAGAG TGTGCTGTTG ATGCAGGAGA TGGAGCTCGA GAAGAGGATG
    GAGCAGCAGC GGCAGAAGCA CAGGCGGGAG AACCAGGCCC AGGAGGCTCA TCTTGACAAG
    CTCTTGGACC AACTGAGGCA GCAAAGCTAT GAGAATAACC TGAAGTCACA CCTGGAAAAG
    GCCAAGGATT TTCTGAAGAA TATGCAATGC AGGTATGAGG CTTTTCATGG CCTCCTGACA
    AAGGAAGTGA TGGAGTACCC AGTGATCATA CTGAAGGAAC TCAACTCCTA CAGCTCCCAC
    CTCAGCCGAC ACTTCTGTGT TCGTGAAATC TTTGAACAGA ACTTGGAGGG AGAAGTCATT
    TTCCAATTGA GGGAACCAGA GCCACATGAA AAACAGTTCC AGAAGAGAAT GAGAAGACCG
    AGAGGAAAAC GAAGGTCCAA AGCAAACGCC CGCAAAGGTG ACGAAAGCAA CAGTGGGAGA
    ACGAGTTCCC TCAAGCCAGC CGAAGAGATA GAAGAAGAAG AAGATCAAGA GGCCGAGTCC
    TCCACCACAC AAGAGGTGGC CGTGAGCCCA CAGGAGAAGT CCGTACTGAG TGAAGAGATG
    GATGAATCCA GGGAGGACTC TATTTCAGGA CTGGAAGAAA TACAGGTTGA AAGAGACAGC
    TCCTTGAGAT CACCCCCAAA CCAGGCCAGT GTGATAGTTC ACGAAGAAGA AGAGGAGGAG
    AAGAAGGAAA AGGTGGAGGA GGAGAGGGAG GAGGAGGAGG TGGAAGAGGA GGAGGAGGAA
    GAGGAGGAGG AGAAGGAGGA AGAGGAGGAG GAGCGGAGGA GCTTGTCTGT GGATGAGGTC
    AAAGCCCAGG AGGAGAGTCT AGGGGAGATC TCCCAGGAGG AGATGGAGTT CTTCACAACC
    TCCAGTGGGA ACACTTACTT TGTTTTCCTA CCCCTGAAAC AAGAAGAGAA CAGCAAGACA
    CCCCACTCCA ACCTTCCTGC CACTCTCTTC AAGGACATTT CCAGTGCCAG GGTCTTAGAA
    GAAGTGATCA TTCCATCCAG ACTAGTTTTA GAGATTAAGA AACAGCTTCG AACTGGCTTT
    TTTGAGCACT TGGAGAAATG GTTTGACCAA TGTGCCCTCA ATGCTCGGGT CATCGTGGCC
    AGCAAGATTG ATGAGCTGGA TTCAGAACTG GAGCTGCGTC TGCACCTGCA CCAGCCAAGA
    GCCCAGCACG TTGAGAAGGA CATCCACAAT GTCAGAGCAG CTGAGCTCTT GCTTCATCAA
    GAACGGCTGA ACAGCCACTG TGCAGGGGTG ATGGAGACAC TGAGGAAGGA GAGGATGATG
    TTCTGCCAGT TCCAAGAAGA GCAAAACTTG AAGAGCAAAC ACTTCCGCCG CAAGATTTCT
    GACATGGAGC ACATCTTCTT GAACGCCACC AAGAGCCAGA AGCTGGCGAC TCTCAGCAGC
    ACTCTGCAGC GGGAGCTGCT CAGCTACGTC GACGTCATCC AGGTGTCCCT GCGCAGCTTC
    CGCCAGTACC TGGAGGAGAG CATGGGCAAG CTGCGCTACA CCAACATTGA GTTCATCAAG
    CACTGCAGAT TGTTTTCAGA GGGAGGCAAC TTCTCTTCTG AAGAAATTGA CTCACTGTGT
    CATCGACTAG AGAAGGAAGC TGCCCGGATA GAGTTTGTTG AAAATTTAAT CATGATCAAC
    ATGGAAAAGA TGGAGAGTGA TTACCTGGAC CAGGCTAATG AGTTCATCAG CAAGTTTGAA
    AGTAAATTCC ATAACCTGTC TGTGGACCTT ATTTTCATAG AGAAAATCCA GCGCTTGCTG
    ACAAATTTGC AAGTAAACCT CAAGTGCGAG GTGGCAAAAT CCAATTTGCA GACAGAGGGA
    TTAAATTCTT CTCTAGAACA GCTTCAAAGC AAGGTAGAAA TGTGCCGAAG CTTAAAAAGG
    AATAAACAAG TAGTGAGCAC AGAAGACCTC CTTGGCCTTA TTCGAACTTG GAAAGAAAAA
    CTGAGCCAAA GGATTGAGTA TCTCAACTGC AAGCTGGACA TGTTATCCAT GACTCACCTG
    GCCTTTCCTG ACAACGTCTT GACTGACCTG GAGGTAGATA GTGACATCTT GTTATCTTCA
    GAAGTCTATG AAGAAGAGGC TAAGGTAGAC ATGGTCACCC CTGAGTCCTT TGCCCAGCCA
    AGCCGCATGG GGAAGTCCAT GATTGAAGAC CCAGCTGTGG AGGTGGTCAA GAGGATCCTG
    CAGTTTCCTG ACTCAAAGTC CTCAACCCTT CAGTGTGACA AAGAACGCTC CCAGACAGCC
    TTGAGGCGAC TCCGGAACCG AGCAGAGCAC TCCTTGAAGA AGGACTTGTC CATCGCCAGT
    GCCACCTCGG TAGCAAGCGT CACGCGGTAC ACCAAGCCCA ACCGACTGGA TAGAAAGTAC
    CAGGTGCTCG GTGACAAGCC TCCTCCAGCA GAGGATTTTA AAGGCATCGT CCTGAGCCTG
    CTCTGGGAGA GCAACGAGCA CCTGCTGGCG GTCGCGGAGG AGTTTTACCG CAAAGAGAAA
    CGCTCAGTCA CCAGGCCCGA CTGCATGTAC GAAACCTTCG ACCAGTGCGC GGAGAACATC
    TGCAAAAAGA TCCTGGAGTA CCACAGCCAG ACAGACGATT ACTACAACTC CTGCCTCATA
    GAATTACGGG CCCAGATGAG GAGATTTGAG GAGCTTCTGC CCCAGGTGTG TTGGCTGGTG
    ATGGAGAATT TCAAGGAGCA CCATTGGAAA AAATGTTGTG CTTCCACCAA AGAGATCAGA
    GAACAGTTCT TGAATTACCA GGAAGAGCTG GAGAAAAGGA AGGATGAAAA TGCCCAGAAG
    CTCCACCCAA ATCTCGGACA CCCTGCACAG CTCCAAGATA TGGAGTCTCT ATGTCAAGTG
    GAAGAGAAGA GGCAGGGAGA TTTGGACGCC TTGATTGTGG TAACCAAGGA GAAGCTGGAG
    GAATGCACAA GGAAGTACGC CCGGTTTTTC ATAGCCACCT TGGCCACCTT CACCGAGAAG
    TTCCTGCTGC AGTTGGATGA GGTGGTCACC ATTGACGATG TCCAGGTTGC AAAGATGGAG
    CCCCCCAAAC AGAAAACTTC AATCCTAATA CGGAGGAAAC TCACCGGGCT GTCCCTGGAG
    GAAGAGAGTG AGAAGCCCCT GATTGAACGG GGGAGCAGAA AGTGGCCAGG AATCAAACCT
    ACTGAAGTCA CCATCCAAAA CAAGATTCTC CTCCGGAAAA CACCATGTAT CACAACCACC
    AAAACAACAC TGGGCCACCT GGCATCTGTG GAAGCCCGAG ATGCTGTCTA CCTGAAATAT
    TTGGCATCAT TTGAAGAGGA GCTGAAGAAG GTCCAGGAGC ACACCGCACT GCAGGTGAAA
    GAGGCTCAGC GCTGGAAGGA CAGCTGGAAA CACTCCGTGA ACATCATCCA GGGCCTGTAC
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328038.1
    CDS285..5150
    Translation

    Target ORF information:

    RefSeq Version XM_015472552.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG CTAAAAAGCG TGTTGAAGAA ATATGTGGAA
    ATCATAGAGA AAACTTCCTA CCTCATGCAG CCCGACGTGT ATAGGCTGAT CAATAAAGAA
    GCCATGATCA TAAACCACGC CCTGCTGGGC AACCGGAGGG CTCTCGCCAA GCTGTTCGTC
    AACCTGATGG AAGCCAGGCT GCAGCAGGAG CTTGATAGCC ACCGCCGCTG GCAGGGCCTG
    ATGGATGCCT GGAAGGCTCT CAAGAAGGAG TATCTGGTGC AGGATTTCAG TGAGTTCATG
    GCCAGTGAGA GAATCCAGAC TCCTCCGGCT GTGAAGAAAG AGCTTGAGAC CATGATGAAG
    AACCAGAGCA GCCTGCAGCA AAAGCGACTG GACCATCTGT GCACCATCTG TGACCTCCTG
    CCCCCCAATT ACAGCAAAGC TCAGCTAACG GAATGGCGTT CTTCTCTCGA CTCGTTGAAC
    AAGCACATCG ATGCCTACCA CATGGACTGC ATGATGCGGA TCCGCTTGCA GTACGAGAAG
    ATCTGGCAGG AGTGCCTAGC CCAAGTGCAG AAGTGCAGGA AGCAGCTGCT GGACTGGAAG
    GCCTTCACTG AGGAGGAGGC AGAGAGCCTG GTGAGCCCAT CCTTCTTCCA GATGGTGGGA
    TGTCTCCAGA GCAAGGTGGA GGAGGAGCTG GAGGTCTTGG ACAAATCCTT CGAGACCGTG
    GCAAAGCAGA CAGAGCAGCA GAGCTCAGAC CTCTTCAGCT ACTTCCAGGA GGCCGTGAAC
    CTATGGGAGA CACATCAGAG TGTGCTGTTG ATGCAGGAGA TGGAGCTCGA GAAGAGGATG
    GAGCAGCAGC GGCAGAAGCA CAGGCGGGAG AACCAGGCCC AGGAGGCTCA TCTTGACAAG
    CTCTTGGACC AACTGAGGCA GCAAAGCTAT GAGAATAACC TGAAGTCACA CCTGGAAAAG
    GCCAAGGATT TTCTGAAGAA TATGCAATGC AGGTATGAGG CTTTTCATGG CCTCCTGACA
    AAGGAAGTGA TGGAGTACCC AGTGATCATA CTGAAGGAAC TCAACTCCTA CAGCTCCCAC
    CTCAGCCGAC ACTTCTGTGT TCGTGAAATC TTTGAACAGA ACTTGGAGGG AGAAGTCATT
    TTCCAATTGA GGGAACCAGA GCCACATGAA AAACAGTTCC AGAAGAGAAT GAGAAGACCG
    AGAGGAAAAC GAAGGTCCAA AGCAAACGCC CGCAAAGGTG ACGAAAGCAA CAGTGGGAGA
    ACGAGTTCCC TCAAGCCAGC CGAAGAGATA GAAGAAGAAG AAGATCAAGA GGCCGAGTCC
    TCCACCACAC AAGAGGTGGC CGTGAGCCCA CAGGAGAAGT CCGTACTGAG TGAAGAGATG
    GATGAATCCA GGGAGGACTC TATTTCAGGA CTGGAAGAAA TACAGGTTGA AAGAGACAGC
    TCCTTGAGAT CACCCCCAAA CCAGGCCAGT GTGATAGTTC ACGAAGAAGA AGAGGAGGAG
    AAGAAGGAAA AGGTGGAGGA GGAGAGGGAG GAGGAGGAGG TGGAAGAGGA GGAGGAGGAA
    GAGGAGGAGG AGAAGGAGGA AGAGGAGGAG GAGCGGAGGA GCTTGTCTGT GGATGAGGTC
    AAAGCCCAGG AGGAGAGTCT AGGGGAGATC TCCCAGGAGG AGATGGAGTT CTTCACAACC
    TCCAGTGGGA ACACTTACTT TGTTTTCCTA CCCCTGAAAC AAGAAGAGAA CAGCAAGACA
    CCCCACTCCA ACCTTCCTGC CACTCTCTTC AAGGACATTT CCAGTGCCAG GGTCTTAGAA
    GAAGTGATCA TTCCATCCAG ACTAGTTTTA GAGATTAAGA AACAGCTTCG AACTGGCTTT
    TTTGAGCACT TGGAGAAATG GTTTGACCAA TGTGCCCTCA ATGCTCGGGT CATCGTGGCC
    AGCAAGATTG ATGAGCTGGA TTCAGAACTG GAGCTGCGTC TGCACCTGCA CCAGCCAAGA
    GCCCAGCACG TTGAGAAGGA CATCCACAAT GTCAGAGCAG CTGAGCTCTT GCTTCATCAA
    GAACGGCTGA ACAGCCACTG TGCAGGGGTG ATGGAGACAC TGAGGAAGGA GAGGATGATG
    TTCTGCCAGT TCCAAGAAGA GCAAAACTTG AAGAGCAAAC ACTTCCGCCG CAAGATTTCT
    GACATGGAGC ACATCTTCTT GAACGCCACC AAGAGCCAGA AGCTGGCGAC TCTCAGCAGC
    ACTCTGCAGC GGGAGCTGCT CAGCTACGTC GACGTCATCC AGGTGTCCCT GCGCAGCTTC
    CGCCAGTACC TGGAGGAGAG CATGGGCAAG CTGCGCTACA CCAACATTGA GTTCATCAAG
    CACTGCAGAT TGTTTTCAGA GGGAGGCAAC TTCTCTTCTG AAGAAATTGA CTCACTGTGT
    CATCGACTAG AGAAGGAAGC TGCCCGGATA GAGTTTGTTG AAAATTTAAT CATGATCAAC
    ATGGAAAAGA TGGAGAGTGA TTACCTGGAC CAGGCTAATG AGTTCATCAG CAAGTTTGAA
    AGTAAATTCC ATAACCTGTC TGTGGACCTT ATTTTCATAG AGAAAATCCA GCGCTTGCTG
    ACAAATTTGC AAGTAAACCT CAAGTGCGAG GTGGCAAAAT CCAATTTGCA GACAGAGGGA
    TTAAATTCTT CTCTAGAACA GCTTCAAAGC AAGGTAGAAA TGTGCCGAAG CTTAAAAAGG
    AATAAACAAG TAGTGAGCAC AGAAGACCTC CTTGGCCTTA TTCGAACTTG GAAAGAAAAA
    CTGAGCCAAA GGATTGAGTA TCTCAACTGC AAGCTGGACA TGTTATCCAT GACTCACCTG
    GCCTTTCCTG ACAACGTCTT GACTGACCTG GAGGTAGATA GTGACATCTT GTTATCTTCA
    GAAGTCTATG AAGAAGAGGC TAAGGTAGAC ATGGTCACCC CTGAGTCCTT TGCCCAGCCA
    AGCCGCATGG GGAAGTCCAT GATTGAAGAC CCAGCTGTGG AGGTGGTCAA GAGGATCCTG
    CAGTTTCCTG ACTCAAAGTC CTCAACCCTT CAGTGTGACA AAGAACGCTC CCAGACAGCC
    TTGAGGCGAC TCCGGAACCG AGCAGAGCAC TCCTTGAAGA AGGACTTGTC CATCGCCAGT
    GCCACCTCGG TAGCAAGCGT CACGCGGTAC ACCAAGCCCA ACCGACTGGA TAGAAAGTAC
    CAGGTGCTCG GTGACAAGCC TCCTCCAGCA GAGGATTTTA AAGGCATCGT CCTGAGCCTG
    CTCTGGGAGA GCAACGAGCA CCTGCTGGCG GTCGCGGAGG AGTTTTACCG CAAAGAGAAA
    CGCTCAGTCA CCAGGCCCGA CTGCATGTAC GAAACCTTCG ACCAGTGCGC GGAGAACATC
    TGCAAAAAGA TCCTGGAGTA CCACAGCCAG ACAGACGATT ACTACAACTC CTGCCTCATA
    GAATTACGGG CCCAGATGAG GAGATTTGAG GAGCTTCTGC CCCAGGTGTG TTGGCTGGTG
    ATGGAGAATT TCAAGGAGCA CCATTGGAAA AAATGTTGTG CTTCCACCAA AGAGATCAGA
    GAACAGTTCT TGAATTACCA GGAAGAGCTG GAGAAAAGGA AGGATGAAAA TGCCCAGAAG
    CTCCACCCAA ATCTCGGACA CCCTGCACAG CTCCAAGATA TGGAGTCTCT ATGTCAAGTG
    GAAGAGAAGA GGCAGGGAGA TTTGGACGCC TTGATTGTGG TAACCAAGGA GAAGCTGGAG
    GAATGCACAA GGAAGTACGC CCGGTTTTTC ATAGCCACCT TGGCCACCTT CACCGAGAAG
    TTCCTGCTGC AGTTGGATGA GGTGGTCACC ATTGACGATG TCCAGGTTGC AAAGATGGAG
    CCCCCCAAAC AGAAAACTTC AATCCTAATA CGGAGGAAAC TCACCGGGCT GTCCCTGGAG
    GAAGAGAGTG AGAAGCCCCT GATTGAACGG GGGAGCAGAA AGTGGCCAGG AATCAAACCT
    ACTGAAGTCA CCATCCAAAA CAAGATTCTC CTCCGGAAAA CACCATGTAT CACAACCACC
    AAAACAACAC TGGGCCACCT GGCATCTGTG GAAGCCCGAG ATGCTGTCTA CCTGAAATAT
    TTGGCATCAT TTGAAGAGGA GCTGAAGAAG GTCCAGGAGC ACACCGCACT GCAGGTGAAA
    GAGGCTCAGC GCTGGAAGGA CAGCTGGAAA CACTCCGTGA ACATCATCCA GGGCCTGTAC
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91289
    Clone ID Related Accession (Same CDS sequence) XM_015472552.1 , XM_015464563.1
    Accession Version XM_015464563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4866bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG CTAAAAAGCG TGTTGAAGAA ATATGTGGAA
    ATCATAGAGA AAACTTCCTA CCTCATGCAG CCCGACGTGT ATAGGCTGAT CAATAAAGAA
    GCCATGATCA TAAACCACGC CCTGCTGGGC AACCGGAGGG CTCTCGCCAA GCTGTTCGTC
    AACCTGATGG AAGCCAGGCT GCAGCAGGAG CTTGATAGCC ACCGCCGCTG GCAGGGCCTG
    ATGGATGCCT GGAAGGCTCT CAAGAAGGAG TATCTGGTGC AGGATTTCAG TGAGTTCATG
    GCCAGTGAGA GAATCCAGAC TCCTCCGGCT GTGAAGAAAG AGCTTGAGAC CATGATGAAG
    AACCAGAGCA GCCTGCAGCA AAAGCGACTG GACCATCTGT GCACCATCTG TGACCTCCTG
    CCCCCCAATT ACAGCAAAGC TCAGCTAACG GAATGGCGTT CTTCTCTCGA CTCGTTGAAC
    AAGCACATCG ATGCCTACCA CATGGACTGC ATGATGCGGA TCCGCTTGCA GTACGAGAAG
    ATCTGGCAGG AGTGCCTAGC CCAAGTGCAG AAGTGCAGGA AGCAGCTGCT GGACTGGAAG
    GCCTTCACTG AGGAGGAGGC AGAGAGCCTG GTGAGCCCAT CCTTCTTCCA GATGGTGGGA
    TGTCTCCAGA GCAAGGTGGA GGAGGAGCTG GAGGTCTTGG ACAAATCCTT CGAGACCGTG
    GCAAAGCAGA CAGAGCAGCA GAGCTCAGAC CTCTTCAGCT ACTTCCAGGA GGCCGTGAAC
    CTATGGGAGA CACATCAGAG TGTGCTGTTG ATGCAGGAGA TGGAGCTCGA GAAGAGGATG
    GAGCAGCAGC GGCAGAAGCA CAGGCGGGAG AACCAGGCCC AGGAGGCTCA TCTTGACAAG
    CTCTTGGACC AACTGAGGCA GCAAAGCTAT GAGAATAACC TGAAGTCACA CCTGGAAAAG
    GCCAAGGATT TTCTGAAGAA TATGCAATGC AGGTATGAGG CTTTTCATGG CCTCCTGACA
    AAGGAAGTGA TGGAGTACCC AGTGATCATA CTGAAGGAAC TCAACTCCTA CAGCTCCCAC
    CTCAGCCGAC ACTTCTGTGT TCGTGAAATC TTTGAACAGA ACTTGGAGGG AGAAGTCATT
    TTCCAATTGA GGGAACCAGA GCCACATGAA AAACAGTTCC AGAAGAGAAT GAGAAGACCG
    AGAGGAAAAC GAAGGTCCAA AGCAAACGCC CGCAAAGGTG ACGAAAGCAA CAGTGGGAGA
    ACGAGTTCCC TCAAGCCAGC CGAAGAGATA GAAGAAGAAG AAGATCAAGA GGCCGAGTCC
    TCCACCACAC AAGAGGTGGC CGTGAGCCCA CAGGAGAAGT CCGTACTGAG TGAAGAGATG
    GATGAATCCA GGGAGGACTC TATTTCAGGA CTGGAAGAAA TACAGGTTGA AAGAGACAGC
    TCCTTGAGAT CACCCCCAAA CCAGGCCAGT GTGATAGTTC ACGAAGAAGA AGAGGAGGAG
    AAGAAGGAAA AGGTGGAGGA GGAGAGGGAG GAGGAGGAGG TGGAAGAGGA GGAGGAGGAA
    GAGGAGGAGG AGAAGGAGGA AGAGGAGGAG GAGCGGAGGA GCTTGTCTGT GGATGAGGTC
    AAAGCCCAGG AGGAGAGTCT AGGGGAGATC TCCCAGGAGG AGATGGAGTT CTTCACAACC
    TCCAGTGGGA ACACTTACTT TGTTTTCCTA CCCCTGAAAC AAGAAGAGAA CAGCAAGACA
    CCCCACTCCA ACCTTCCTGC CACTCTCTTC AAGGACATTT CCAGTGCCAG GGTCTTAGAA
    GAAGTGATCA TTCCATCCAG ACTAGTTTTA GAGATTAAGA AACAGCTTCG AACTGGCTTT
    TTTGAGCACT TGGAGAAATG GTTTGACCAA TGTGCCCTCA ATGCTCGGGT CATCGTGGCC
    AGCAAGATTG ATGAGCTGGA TTCAGAACTG GAGCTGCGTC TGCACCTGCA CCAGCCAAGA
    GCCCAGCACG TTGAGAAGGA CATCCACAAT GTCAGAGCAG CTGAGCTCTT GCTTCATCAA
    GAACGGCTGA ACAGCCACTG TGCAGGGGTG ATGGAGACAC TGAGGAAGGA GAGGATGATG
    TTCTGCCAGT TCCAAGAAGA GCAAAACTTG AAGAGCAAAC ACTTCCGCCG CAAGATTTCT
    GACATGGAGC ACATCTTCTT GAACGCCACC AAGAGCCAGA AGCTGGCGAC TCTCAGCAGC
    ACTCTGCAGC GGGAGCTGCT CAGCTACGTC GACGTCATCC AGGTGTCCCT GCGCAGCTTC
    CGCCAGTACC TGGAGGAGAG CATGGGCAAG CTGCGCTACA CCAACATTGA GTTCATCAAG
    CACTGCAGAT TGTTTTCAGA GGGAGGCAAC TTCTCTTCTG AAGAAATTGA CTCACTGTGT
    CATCGACTAG AGAAGGAAGC TGCCCGGATA GAGTTTGTTG AAAATTTAAT CATGATCAAC
    ATGGAAAAGA TGGAGAGTGA TTACCTGGAC CAGGCTAATG AGTTCATCAG CAAGTTTGAA
    AGTAAATTCC ATAACCTGTC TGTGGACCTT ATTTTCATAG AGAAAATCCA GCGCTTGCTG
    ACAAATTTGC AAGTAAACCT CAAGTGCGAG GTGGCAAAAT CCAATTTGCA GACAGAGGGA
    TTAAATTCTT CTCTAGAACA GCTTCAAAGC AAGGTAGAAA TGTGCCGAAG CTTAAAAAGG
    AATAAACAAG TAGTGAGCAC AGAAGACCTC CTTGGCCTTA TTCGAACTTG GAAAGAAAAA
    CTGAGCCAAA GGATTGAGTA TCTCAACTGC AAGCTGGACA TGTTATCCAT GACTCACCTG
    GCCTTTCCTG ACAACGTCTT GACTGACCTG GAGGTAGATA GTGACATCTT GTTATCTTCA
    GAAGTCTATG AAGAAGAGGC TAAGGTAGAC ATGGTCACCC CTGAGTCCTT TGCCCAGCCA
    AGCCGCATGG GGAAGTCCAT GATTGAAGAC CCAGCTGTGG AGGTGGTCAA GAGGATCCTG
    CAGTTTCCTG ACTCAAAGTC CTCAACCCTT CAGTGTGACA AAGAACGCTC CCAGACAGCC
    TTGAGGCGAC TCCGGAACCG AGCAGAGCAC TCCTTGAAGA AGGACTTGTC CATCGCCAGT
    GCCACCTCGG TAGCAAGCGT CACGCGGTAC ACCAAGCCCA ACCGACTGGA TAGAAAGTAC
    CAGGTGCTCG GTGACAAGCC TCCTCCAGCA GAGGATTTTA AAGGCATCGT CCTGAGCCTG
    CTCTGGGAGA GCAACGAGCA CCTGCTGGCG GTCGCGGAGG AGTTTTACCG CAAAGAGAAA
    CGCTCAGTCA CCAGGCCCGA CTGCATGTAC GAAACCTTCG ACCAGTGCGC GGAGAACATC
    TGCAAAAAGA TCCTGGAGTA CCACAGCCAG ACAGACGATT ACTACAACTC CTGCCTCATA
    GAATTACGGG CCCAGATGAG GAGATTTGAG GAGCTTCTGC CCCAGGTGTG TTGGCTGGTG
    ATGGAGAATT TCAAGGAGCA CCATTGGAAA AAATGTTGTG CTTCCACCAA AGAGATCAGA
    GAACAGTTCT TGAATTACCA GGAAGAGCTG GAGAAAAGGA AGGATGAAAA TGCCCAGAAG
    CTCCACCCAA ATCTCGGACA CCCTGCACAG CTCCAAGATA TGGAGTCTCT ATGTCAAGTG
    GAAGAGAAGA GGCAGGGAGA TTTGGACGCC TTGATTGTGG TAACCAAGGA GAAGCTGGAG
    GAATGCACAA GGAAGTACGC CCGGTTTTTC ATAGCCACCT TGGCCACCTT CACCGAGAAG
    TTCCTGCTGC AGTTGGATGA GGTGGTCACC ATTGACGATG TCCAGGTTGC AAAGATGGAG
    CCCCCCAAAC AGAAAACTTC AATCCTAATA CGGAGGAAAC TCACCGGGCT GTCCCTGGAG
    GAAGAGAGTG AGAAGCCCCT GATTGAACGG GGGAGCAGAA AGTGGCCAGG AATCAAACCT
    ACTGAAGTCA CCATCCAAAA CAAGATTCTC CTCCGGAAAA CACCATGTAT CACAACCACC
    AAAACAACAC TGGGCCACCT GGCATCTGTG GAAGCCCGAG ATGCTGTCTA CCTGAAATAT
    TTGGCATCAT TTGAAGAGGA GCTGAAGAAG GTCCAGGAGC ACACCGCACT GCAGGTGAAA
    GAGGCTCAGC GCTGGAAGGA CAGCTGGAAA CACTCCGTGA ACATCATCCA GGGCCTGTAC
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320049.1
    CDS285..5150
    Translation

    Target ORF information:

    RefSeq Version XM_015464563.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG CTAAAAAGCG TGTTGAAGAA ATATGTGGAA
    ATCATAGAGA AAACTTCCTA CCTCATGCAG CCCGACGTGT ATAGGCTGAT CAATAAAGAA
    GCCATGATCA TAAACCACGC CCTGCTGGGC AACCGGAGGG CTCTCGCCAA GCTGTTCGTC
    AACCTGATGG AAGCCAGGCT GCAGCAGGAG CTTGATAGCC ACCGCCGCTG GCAGGGCCTG
    ATGGATGCCT GGAAGGCTCT CAAGAAGGAG TATCTGGTGC AGGATTTCAG TGAGTTCATG
    GCCAGTGAGA GAATCCAGAC TCCTCCGGCT GTGAAGAAAG AGCTTGAGAC CATGATGAAG
    AACCAGAGCA GCCTGCAGCA AAAGCGACTG GACCATCTGT GCACCATCTG TGACCTCCTG
    CCCCCCAATT ACAGCAAAGC TCAGCTAACG GAATGGCGTT CTTCTCTCGA CTCGTTGAAC
    AAGCACATCG ATGCCTACCA CATGGACTGC ATGATGCGGA TCCGCTTGCA GTACGAGAAG
    ATCTGGCAGG AGTGCCTAGC CCAAGTGCAG AAGTGCAGGA AGCAGCTGCT GGACTGGAAG
    GCCTTCACTG AGGAGGAGGC AGAGAGCCTG GTGAGCCCAT CCTTCTTCCA GATGGTGGGA
    TGTCTCCAGA GCAAGGTGGA GGAGGAGCTG GAGGTCTTGG ACAAATCCTT CGAGACCGTG
    GCAAAGCAGA CAGAGCAGCA GAGCTCAGAC CTCTTCAGCT ACTTCCAGGA GGCCGTGAAC
    CTATGGGAGA CACATCAGAG TGTGCTGTTG ATGCAGGAGA TGGAGCTCGA GAAGAGGATG
    GAGCAGCAGC GGCAGAAGCA CAGGCGGGAG AACCAGGCCC AGGAGGCTCA TCTTGACAAG
    CTCTTGGACC AACTGAGGCA GCAAAGCTAT GAGAATAACC TGAAGTCACA CCTGGAAAAG
    GCCAAGGATT TTCTGAAGAA TATGCAATGC AGGTATGAGG CTTTTCATGG CCTCCTGACA
    AAGGAAGTGA TGGAGTACCC AGTGATCATA CTGAAGGAAC TCAACTCCTA CAGCTCCCAC
    CTCAGCCGAC ACTTCTGTGT TCGTGAAATC TTTGAACAGA ACTTGGAGGG AGAAGTCATT
    TTCCAATTGA GGGAACCAGA GCCACATGAA AAACAGTTCC AGAAGAGAAT GAGAAGACCG
    AGAGGAAAAC GAAGGTCCAA AGCAAACGCC CGCAAAGGTG ACGAAAGCAA CAGTGGGAGA
    ACGAGTTCCC TCAAGCCAGC CGAAGAGATA GAAGAAGAAG AAGATCAAGA GGCCGAGTCC
    TCCACCACAC AAGAGGTGGC CGTGAGCCCA CAGGAGAAGT CCGTACTGAG TGAAGAGATG
    GATGAATCCA GGGAGGACTC TATTTCAGGA CTGGAAGAAA TACAGGTTGA AAGAGACAGC
    TCCTTGAGAT CACCCCCAAA CCAGGCCAGT GTGATAGTTC ACGAAGAAGA AGAGGAGGAG
    AAGAAGGAAA AGGTGGAGGA GGAGAGGGAG GAGGAGGAGG TGGAAGAGGA GGAGGAGGAA
    GAGGAGGAGG AGAAGGAGGA AGAGGAGGAG GAGCGGAGGA GCTTGTCTGT GGATGAGGTC
    AAAGCCCAGG AGGAGAGTCT AGGGGAGATC TCCCAGGAGG AGATGGAGTT CTTCACAACC
    TCCAGTGGGA ACACTTACTT TGTTTTCCTA CCCCTGAAAC AAGAAGAGAA CAGCAAGACA
    CCCCACTCCA ACCTTCCTGC CACTCTCTTC AAGGACATTT CCAGTGCCAG GGTCTTAGAA
    GAAGTGATCA TTCCATCCAG ACTAGTTTTA GAGATTAAGA AACAGCTTCG AACTGGCTTT
    TTTGAGCACT TGGAGAAATG GTTTGACCAA TGTGCCCTCA ATGCTCGGGT CATCGTGGCC
    AGCAAGATTG ATGAGCTGGA TTCAGAACTG GAGCTGCGTC TGCACCTGCA CCAGCCAAGA
    GCCCAGCACG TTGAGAAGGA CATCCACAAT GTCAGAGCAG CTGAGCTCTT GCTTCATCAA
    GAACGGCTGA ACAGCCACTG TGCAGGGGTG ATGGAGACAC TGAGGAAGGA GAGGATGATG
    TTCTGCCAGT TCCAAGAAGA GCAAAACTTG AAGAGCAAAC ACTTCCGCCG CAAGATTTCT
    GACATGGAGC ACATCTTCTT GAACGCCACC AAGAGCCAGA AGCTGGCGAC TCTCAGCAGC
    ACTCTGCAGC GGGAGCTGCT CAGCTACGTC GACGTCATCC AGGTGTCCCT GCGCAGCTTC
    CGCCAGTACC TGGAGGAGAG CATGGGCAAG CTGCGCTACA CCAACATTGA GTTCATCAAG
    CACTGCAGAT TGTTTTCAGA GGGAGGCAAC TTCTCTTCTG AAGAAATTGA CTCACTGTGT
    CATCGACTAG AGAAGGAAGC TGCCCGGATA GAGTTTGTTG AAAATTTAAT CATGATCAAC
    ATGGAAAAGA TGGAGAGTGA TTACCTGGAC CAGGCTAATG AGTTCATCAG CAAGTTTGAA
    AGTAAATTCC ATAACCTGTC TGTGGACCTT ATTTTCATAG AGAAAATCCA GCGCTTGCTG
    ACAAATTTGC AAGTAAACCT CAAGTGCGAG GTGGCAAAAT CCAATTTGCA GACAGAGGGA
    TTAAATTCTT CTCTAGAACA GCTTCAAAGC AAGGTAGAAA TGTGCCGAAG CTTAAAAAGG
    AATAAACAAG TAGTGAGCAC AGAAGACCTC CTTGGCCTTA TTCGAACTTG GAAAGAAAAA
    CTGAGCCAAA GGATTGAGTA TCTCAACTGC AAGCTGGACA TGTTATCCAT GACTCACCTG
    GCCTTTCCTG ACAACGTCTT GACTGACCTG GAGGTAGATA GTGACATCTT GTTATCTTCA
    GAAGTCTATG AAGAAGAGGC TAAGGTAGAC ATGGTCACCC CTGAGTCCTT TGCCCAGCCA
    AGCCGCATGG GGAAGTCCAT GATTGAAGAC CCAGCTGTGG AGGTGGTCAA GAGGATCCTG
    CAGTTTCCTG ACTCAAAGTC CTCAACCCTT CAGTGTGACA AAGAACGCTC CCAGACAGCC
    TTGAGGCGAC TCCGGAACCG AGCAGAGCAC TCCTTGAAGA AGGACTTGTC CATCGCCAGT
    GCCACCTCGG TAGCAAGCGT CACGCGGTAC ACCAAGCCCA ACCGACTGGA TAGAAAGTAC
    CAGGTGCTCG GTGACAAGCC TCCTCCAGCA GAGGATTTTA AAGGCATCGT CCTGAGCCTG
    CTCTGGGAGA GCAACGAGCA CCTGCTGGCG GTCGCGGAGG AGTTTTACCG CAAAGAGAAA
    CGCTCAGTCA CCAGGCCCGA CTGCATGTAC GAAACCTTCG ACCAGTGCGC GGAGAACATC
    TGCAAAAAGA TCCTGGAGTA CCACAGCCAG ACAGACGATT ACTACAACTC CTGCCTCATA
    GAATTACGGG CCCAGATGAG GAGATTTGAG GAGCTTCTGC CCCAGGTGTG TTGGCTGGTG
    ATGGAGAATT TCAAGGAGCA CCATTGGAAA AAATGTTGTG CTTCCACCAA AGAGATCAGA
    GAACAGTTCT TGAATTACCA GGAAGAGCTG GAGAAAAGGA AGGATGAAAA TGCCCAGAAG
    CTCCACCCAA ATCTCGGACA CCCTGCACAG CTCCAAGATA TGGAGTCTCT ATGTCAAGTG
    GAAGAGAAGA GGCAGGGAGA TTTGGACGCC TTGATTGTGG TAACCAAGGA GAAGCTGGAG
    GAATGCACAA GGAAGTACGC CCGGTTTTTC ATAGCCACCT TGGCCACCTT CACCGAGAAG
    TTCCTGCTGC AGTTGGATGA GGTGGTCACC ATTGACGATG TCCAGGTTGC AAAGATGGAG
    CCCCCCAAAC AGAAAACTTC AATCCTAATA CGGAGGAAAC TCACCGGGCT GTCCCTGGAG
    GAAGAGAGTG AGAAGCCCCT GATTGAACGG GGGAGCAGAA AGTGGCCAGG AATCAAACCT
    ACTGAAGTCA CCATCCAAAA CAAGATTCTC CTCCGGAAAA CACCATGTAT CACAACCACC
    AAAACAACAC TGGGCCACCT GGCATCTGTG GAAGCCCGAG ATGCTGTCTA CCTGAAATAT
    TTGGCATCAT TTGAAGAGGA GCTGAAGAAG GTCCAGGAGC ACACCGCACT GCAGGTGAAA
    GAGGCTCAGC GCTGGAAGGA CAGCTGGAAA CACTCCGTGA ACATCATCCA GGGCCTGTAC
    TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91290
    Clone ID Related Accession (Same CDS sequence) XM_015472553.1 , XM_015464564.1
    Accession Version XM_015472553.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATCCCAA AGGACAGCAG GAAGCCCTTG GTGAAGAAGG TGGATGTCTG TGAAGGCGAG 
    ATGATGTCTC CTCGGCAGCG GAAGTGGATG CGCAGCCTAC CCAATGACTG GACCACAGAA
    AACCCTGTTC TTTACAGGGA AAAGCAAATA GCCAAAAAGG AAAAAGCTCA AGAGAGTGAG
    AACGCCATCG ACGCCAGAGA AGTCCGGGGC CTCACGGACA CCATTGTTCC TGAGAGGATC
    AGCAGCACCA CCTTTCAAAA GCAAGGAGAT TCCAAGAGGA GGGGCTACGA GAGTGCCCTG
    GCGAGCTTTA AGGAGGAGAT CGCTCAGATT GGGATGGAAA TGGAACCTCT CATCTTGGAA
    CCAGGAGCAC TTCTTTTAAA AAAGCTGGCT GAGTCTGATG AGGATGTGAA CAATCTCTTC
    AAAAAGGTGG AAAACGACAG CAACCTCGAA GACTACACCA TCCAAACCCT GTTGGAGCTG
    AGGGATCAGG TGGCCGAGAA GTTCCTGCTG CAGAAGCAGG AGATCAAGGA GCTGGATGAG
    ACACTGCACT CACTTGAGTT CTCCCGGGTA GATAAACTAA AAAGCGTGTT GAAGAAATAT
    GTGGAAATCA TAGAGAAAAC TTCCTACCTC ATGCAGCCCG ACGTGTATAG GCTGATCAAT
    AAAGAAGCCA TGATCATAAA CCACGCCCTG CTGGGCAACC GGAGGGCTCT CGCCAAGCTG
    TTCGTCAACC TGATGGAAGC CAGGCTGCAG CAGGAGCTTG ATAGCCACCG CCGCTGGCAG
    GGCCTGATGG ATGCCTGGAA GGCTCTCAAG AAGGAGTATC TGGTGCAGGA TTTCAGTGAG
    TTCATGGCCA GTGAGAGAAT CCAGACTCCT CCGGCTGTGA AGAAAGAGCT TGAGACCATG
    ATGAAGAACC AGAGCAGCCT GCAGCAAAAG CGACTGGACC ATCTGTGCAC CATCTGTGAC
    CTCCTGCCCC CCAATTACAG CAAAGCTCAG CTAACGGAAT GGCGTTCTTC TCTCGACTCG
    TTGAACAAGC ACATCGATGC CTACCACATG GACTGCATGA TGCGGATCCG CTTGCAGTAC
    GAGAAGATCT GGCAGGAGTG CCTAGCCCAA GTGCAGAAGT GCAGGAAGCA GCTGCTGGAC
    TGGAAGGCCT TCACTGAGGA GGAGGCAGAG AGCCTGGTGA GCCCATCCTT CTTCCAGATG
    GTGGGATGTC TCCAGAGCAA GGTGGAGGAG GAGCTGGAGG TCTTGGACAA ATCCTTCGAG
    ACCGTGGCAA AGCAGACAGA GCAGCAGAGC TCAGACCTCT TCAGCTACTT CCAGGAGGCC
    GTGAACCTAT GGGAGACACA TCAGAGTGTG CTGTTGATGC AGGAGATGGA GCTCGAGAAG
    AGGATGGAGC AGCAGCGGCA GAAGCACAGG CGGGAGAACC AGGCCCAGGA GGCTCATCTT
    GACAAGCTCT TGGACCAACT GAGGCAGCAA AGCTATGAGA ATAACCTGAA GTCACACCTG
    GAAAAGGCCA AGGATTTTCT GAAGAATATG CAATGCAGGT ATGAGGCTTT TCATGGCCTC
    CTGACAAAGG AAGTGATGGA GTACCCAGTG ATCATACTGA AGGAACTCAA CTCCTACAGC
    TCCCACCTCA GCCGACACTT CTGTGTTCGT GAAATCTTTG AACAGAACTT GGAGGGAGAA
    GTCATTTTCC AATTGAGGGA ACCAGAGCCA CATGAAAAAC AGTTCCAGAA GAGAATGAGA
    AGACCGAGAG GAAAACGAAG GTCCAAAGCA AACGCCCGCA AAGGTGACGA AAGCAACAGT
    GGGAGAACGA GTTCCCTCAA GCCAGCCGAA GAGATAGAAG AAGAAGAAGA TCAAGAGGCC
    GAGTCCTCCA CCACACAAGA GGTGGCCGTG AGCCCACAGG AGAAGTCCGT ACTGAGTGAA
    GAGATGGATG AATCCAGGGA GGACTCTATT TCAGGACTGG AAGAAATACA GGTTGAAAGA
    GACAGCTCCT TGAGATCACC CCCAAACCAG GCCAGTGTGA TAGTTCACGA AGAAGAAGAG
    GAGGAGAAGA AGGAAAAGGT GGAGGAGGAG AGGGAGGAGG AGGAGGTGGA AGAGGAGGAG
    GAGGAAGAGG AGGAGGAGAA GGAGGAAGAG GAGGAGGAGC GGAGGAGCTT GTCTGTGGAT
    GAGGTCAAAG CCCAGGAGGA GAGTCTAGGG GAGATCTCCC AGGAGGAGAT GGAGTTCTTC
    ACAACCTCCA GTGGGAACAC TTACTTTGTT TTCCTACCCC TGAAACAAGA AGAGAACAGC
    AAGACACCCC ACTCCAACCT TCCTGCCACT CTCTTCAAGG ACATTTCCAG TGCCAGGGTC
    TTAGAAGAAG TGATCATTCC ATCCAGACTA GTTTTAGAGA TTAAGAAACA GCTTCGAACT
    GGCTTTTTTG AGCACTTGGA GAAATGGTTT GACCAATGTG CCCTCAATGC TCGGGTCATC
    GTGGCCAGCA AGATTGATGA GCTGGATTCA GAACTGGAGC TGCGTCTGCA CCTGCACCAG
    CCAAGAGCCC AGCACGTTGA GAAGGACATC CACAATGTCA GAGCAGCTGA GCTCTTGCTT
    CATCAAGAAC GGCTGAACAG CCACTGTGCA GGGGTGATGG AGACACTGAG GAAGGAGAGG
    ATGATGTTCT GCCAGTTCCA AGAAGAGCAA AACTTGAAGA GCAAACACTT CCGCCGCAAG
    ATTTCTGACA TGGAGCACAT CTTCTTGAAC GCCACCAAGA GCCAGAAGCT GGCGACTCTC
    AGCAGCACTC TGCAGCGGGA GCTGCTCAGC TACGTCGACG TCATCCAGGT GTCCCTGCGC
    AGCTTCCGCC AGTACCTGGA GGAGAGCATG GGCAAGCTGC GCTACACCAA CATTGAGTTC
    ATCAAGCACT GCAGATTGTT TTCAGAGGGA GGCAACTTCT CTTCTGAAGA AATTGACTCA
    CTGTGTCATC GACTAGAGAA GGAAGCTGCC CGGATAGAGT TTGTTGAAAA TTTAATCATG
    ATCAACATGG AAAAGATGGA GAGTGATTAC CTGGACCAGG CTAATGAGTT CATCAGCAAG
    TTTGAAAGTA AATTCCATAA CCTGTCTGTG GACCTTATTT TCATAGAGAA AATCCAGCGC
    TTGCTGACAA ATTTGCAAGT AAACCTCAAG TGCGAGGTGG CAAAATCCAA TTTGCAGACA
    GAGGGATTAA ATTCTTCTCT AGAACAGCTT CAAAGCAAGG TAGAAATGTG CCGAAGCTTA
    AAAAGGAATA AACAAGTAGT GAGCACAGAA GACCTCCTTG GCCTTATTCG AACTTGGAAA
    GAAAAACTGA GCCAAAGGAT TGAGTATCTC AACTGCAAGC TGGACATGTT ATCCATGACT
    CACCTGGCCT TTCCTGACAA CGTCTTGACT GACCTGGAGG TAGATAGTGA CATCTTGTTA
    TCTTCAGAAG TCTATGAAGA AGAGGCTAAG GTAGACATGG TCACCCCTGA GTCCTTTGCC
    CAGCCAAGCC GCATGGGGAA GTCCATGATT GAAGACCCAG CTGTGGAGGT GGTCAAGAGG
    ATCCTGCAGT TTCCTGACTC AAAGTCCTCA ACCCTTCAGT GTGACAAAGA ACGCTCCCAG
    ACAGCCTTGA GGCGACTCCG GAACCGAGCA GAGCACTCCT TGAAGAAGGA CTTGTCCATC
    GCCAGTGCCA CCTCGGTAGC AAGCGTCACG CGGTACACCA AGCCCAACCG ACTGGATAGA
    AAGTACCAGG TGCTCGGTGA CAAGCCTCCT CCAGCAGAGG ATTTTAAAGG CATCGTCCTG
    AGCCTGCTCT GGGAGAGCAA CGAGCACCTG CTGGCGGTCG CGGAGGAGTT TTACCGCAAA
    GAGAAACGCT CAGTCACCAG GCCCGACTGC ATGTACGAAA CCTTCGACCA GTGCGCGGAG
    AACATCTGCA AAAAGATCCT GGAGTACCAC AGCCAGACAG ACGATTACTA CAACTCCTGC
    CTCATAGAAT TACGGGCCCA GATGAGGAGA TTTGAGGAGC TTCTGCCCCA GGTGTGTTGG
    CTGGTGATGG AGAATTTCAA GGAGCACCAT TGGAAAAAAT GTTGTGCTTC CACCAAAGAG
    ATCAGAGAAC AGTTCTTGAA TTACCAGGAA GAGCTGGAGA AAAGGAAGGA TGAAAATGCC
    CAGAAGCTCC ACCCAAATCT CGGACACCCT GCACAGCTCC AAGATATGGA GTCTCTATGT
    CAAGTGGAAG AGAAGAGGCA GGGAGATTTG GACGCCTTGA TTGTGGTAAC CAAGGAGAAG
    CTGGAGGAAT GCACAAGGAA GTACGCCCGG TTTTTCATAG CCACCTTGGC CACCTTCACC
    GAGAAGTTCC TGCTGCAGTT GGATGAGGTG GTCACCATTG ACGATGTCCA GGTTGCAAAG
    ATGGAGCCCC CCAAACAGAA AACTTCAATC CTAATACGGA GGAAACTCAC CGGGCTGTCC
    CTGGAGGAAG AGAGTGAGAA GCCCCTGATT GAACGGGGGA GCAGAAAGTG GCCAGGAATC
    AAACCTACTG AAGTCACCAT CCAAAACAAG ATTCTCCTCC GGAAAACACC ATGTATCACA
    ACCACCAAAA CAACACTGGG CCACCTGGCA TCTGTGGAAG CCCGAGATGC TGTCTACCTG
    AAATATTTGG CATCATTTGA AGAGGAGCTG AAGAAGGTCC AGGAGCACAC CGCACTGCAG
    GTGAAAGAGG CTCAGCGCTG GAAGGACAGC TGGAAACACT CCGTGAACAT CATCCAGGGC
    CTGTACTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328039.1
    CDS637..5388
    Translation

    Target ORF information:

    RefSeq Version XM_015472553.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472553.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATCCCAA AGGACAGCAG GAAGCCCTTG GTGAAGAAGG TGGATGTCTG TGAAGGCGAG 
    ATGATGTCTC CTCGGCAGCG GAAGTGGATG CGCAGCCTAC CCAATGACTG GACCACAGAA
    AACCCTGTTC TTTACAGGGA AAAGCAAATA GCCAAAAAGG AAAAAGCTCA AGAGAGTGAG
    AACGCCATCG ACGCCAGAGA AGTCCGGGGC CTCACGGACA CCATTGTTCC TGAGAGGATC
    AGCAGCACCA CCTTTCAAAA GCAAGGAGAT TCCAAGAGGA GGGGCTACGA GAGTGCCCTG
    GCGAGCTTTA AGGAGGAGAT CGCTCAGATT GGGATGGAAA TGGAACCTCT CATCTTGGAA
    CCAGGAGCAC TTCTTTTAAA AAAGCTGGCT GAGTCTGATG AGGATGTGAA CAATCTCTTC
    AAAAAGGTGG AAAACGACAG CAACCTCGAA GACTACACCA TCCAAACCCT GTTGGAGCTG
    AGGGATCAGG TGGCCGAGAA GTTCCTGCTG CAGAAGCAGG AGATCAAGGA GCTGGATGAG
    ACACTGCACT CACTTGAGTT CTCCCGGGTA GATAAACTAA AAAGCGTGTT GAAGAAATAT
    GTGGAAATCA TAGAGAAAAC TTCCTACCTC ATGCAGCCCG ACGTGTATAG GCTGATCAAT
    AAAGAAGCCA TGATCATAAA CCACGCCCTG CTGGGCAACC GGAGGGCTCT CGCCAAGCTG
    TTCGTCAACC TGATGGAAGC CAGGCTGCAG CAGGAGCTTG ATAGCCACCG CCGCTGGCAG
    GGCCTGATGG ATGCCTGGAA GGCTCTCAAG AAGGAGTATC TGGTGCAGGA TTTCAGTGAG
    TTCATGGCCA GTGAGAGAAT CCAGACTCCT CCGGCTGTGA AGAAAGAGCT TGAGACCATG
    ATGAAGAACC AGAGCAGCCT GCAGCAAAAG CGACTGGACC ATCTGTGCAC CATCTGTGAC
    CTCCTGCCCC CCAATTACAG CAAAGCTCAG CTAACGGAAT GGCGTTCTTC TCTCGACTCG
    TTGAACAAGC ACATCGATGC CTACCACATG GACTGCATGA TGCGGATCCG CTTGCAGTAC
    GAGAAGATCT GGCAGGAGTG CCTAGCCCAA GTGCAGAAGT GCAGGAAGCA GCTGCTGGAC
    TGGAAGGCCT TCACTGAGGA GGAGGCAGAG AGCCTGGTGA GCCCATCCTT CTTCCAGATG
    GTGGGATGTC TCCAGAGCAA GGTGGAGGAG GAGCTGGAGG TCTTGGACAA ATCCTTCGAG
    ACCGTGGCAA AGCAGACAGA GCAGCAGAGC TCAGACCTCT TCAGCTACTT CCAGGAGGCC
    GTGAACCTAT GGGAGACACA TCAGAGTGTG CTGTTGATGC AGGAGATGGA GCTCGAGAAG
    AGGATGGAGC AGCAGCGGCA GAAGCACAGG CGGGAGAACC AGGCCCAGGA GGCTCATCTT
    GACAAGCTCT TGGACCAACT GAGGCAGCAA AGCTATGAGA ATAACCTGAA GTCACACCTG
    GAAAAGGCCA AGGATTTTCT GAAGAATATG CAATGCAGGT ATGAGGCTTT TCATGGCCTC
    CTGACAAAGG AAGTGATGGA GTACCCAGTG ATCATACTGA AGGAACTCAA CTCCTACAGC
    TCCCACCTCA GCCGACACTT CTGTGTTCGT GAAATCTTTG AACAGAACTT GGAGGGAGAA
    GTCATTTTCC AATTGAGGGA ACCAGAGCCA CATGAAAAAC AGTTCCAGAA GAGAATGAGA
    AGACCGAGAG GAAAACGAAG GTCCAAAGCA AACGCCCGCA AAGGTGACGA AAGCAACAGT
    GGGAGAACGA GTTCCCTCAA GCCAGCCGAA GAGATAGAAG AAGAAGAAGA TCAAGAGGCC
    GAGTCCTCCA CCACACAAGA GGTGGCCGTG AGCCCACAGG AGAAGTCCGT ACTGAGTGAA
    GAGATGGATG AATCCAGGGA GGACTCTATT TCAGGACTGG AAGAAATACA GGTTGAAAGA
    GACAGCTCCT TGAGATCACC CCCAAACCAG GCCAGTGTGA TAGTTCACGA AGAAGAAGAG
    GAGGAGAAGA AGGAAAAGGT GGAGGAGGAG AGGGAGGAGG AGGAGGTGGA AGAGGAGGAG
    GAGGAAGAGG AGGAGGAGAA GGAGGAAGAG GAGGAGGAGC GGAGGAGCTT GTCTGTGGAT
    GAGGTCAAAG CCCAGGAGGA GAGTCTAGGG GAGATCTCCC AGGAGGAGAT GGAGTTCTTC
    ACAACCTCCA GTGGGAACAC TTACTTTGTT TTCCTACCCC TGAAACAAGA AGAGAACAGC
    AAGACACCCC ACTCCAACCT TCCTGCCACT CTCTTCAAGG ACATTTCCAG TGCCAGGGTC
    TTAGAAGAAG TGATCATTCC ATCCAGACTA GTTTTAGAGA TTAAGAAACA GCTTCGAACT
    GGCTTTTTTG AGCACTTGGA GAAATGGTTT GACCAATGTG CCCTCAATGC TCGGGTCATC
    GTGGCCAGCA AGATTGATGA GCTGGATTCA GAACTGGAGC TGCGTCTGCA CCTGCACCAG
    CCAAGAGCCC AGCACGTTGA GAAGGACATC CACAATGTCA GAGCAGCTGA GCTCTTGCTT
    CATCAAGAAC GGCTGAACAG CCACTGTGCA GGGGTGATGG AGACACTGAG GAAGGAGAGG
    ATGATGTTCT GCCAGTTCCA AGAAGAGCAA AACTTGAAGA GCAAACACTT CCGCCGCAAG
    ATTTCTGACA TGGAGCACAT CTTCTTGAAC GCCACCAAGA GCCAGAAGCT GGCGACTCTC
    AGCAGCACTC TGCAGCGGGA GCTGCTCAGC TACGTCGACG TCATCCAGGT GTCCCTGCGC
    AGCTTCCGCC AGTACCTGGA GGAGAGCATG GGCAAGCTGC GCTACACCAA CATTGAGTTC
    ATCAAGCACT GCAGATTGTT TTCAGAGGGA GGCAACTTCT CTTCTGAAGA AATTGACTCA
    CTGTGTCATC GACTAGAGAA GGAAGCTGCC CGGATAGAGT TTGTTGAAAA TTTAATCATG
    ATCAACATGG AAAAGATGGA GAGTGATTAC CTGGACCAGG CTAATGAGTT CATCAGCAAG
    TTTGAAAGTA AATTCCATAA CCTGTCTGTG GACCTTATTT TCATAGAGAA AATCCAGCGC
    TTGCTGACAA ATTTGCAAGT AAACCTCAAG TGCGAGGTGG CAAAATCCAA TTTGCAGACA
    GAGGGATTAA ATTCTTCTCT AGAACAGCTT CAAAGCAAGG TAGAAATGTG CCGAAGCTTA
    AAAAGGAATA AACAAGTAGT GAGCACAGAA GACCTCCTTG GCCTTATTCG AACTTGGAAA
    GAAAAACTGA GCCAAAGGAT TGAGTATCTC AACTGCAAGC TGGACATGTT ATCCATGACT
    CACCTGGCCT TTCCTGACAA CGTCTTGACT GACCTGGAGG TAGATAGTGA CATCTTGTTA
    TCTTCAGAAG TCTATGAAGA AGAGGCTAAG GTAGACATGG TCACCCCTGA GTCCTTTGCC
    CAGCCAAGCC GCATGGGGAA GTCCATGATT GAAGACCCAG CTGTGGAGGT GGTCAAGAGG
    ATCCTGCAGT TTCCTGACTC AAAGTCCTCA ACCCTTCAGT GTGACAAAGA ACGCTCCCAG
    ACAGCCTTGA GGCGACTCCG GAACCGAGCA GAGCACTCCT TGAAGAAGGA CTTGTCCATC
    GCCAGTGCCA CCTCGGTAGC AAGCGTCACG CGGTACACCA AGCCCAACCG ACTGGATAGA
    AAGTACCAGG TGCTCGGTGA CAAGCCTCCT CCAGCAGAGG ATTTTAAAGG CATCGTCCTG
    AGCCTGCTCT GGGAGAGCAA CGAGCACCTG CTGGCGGTCG CGGAGGAGTT TTACCGCAAA
    GAGAAACGCT CAGTCACCAG GCCCGACTGC ATGTACGAAA CCTTCGACCA GTGCGCGGAG
    AACATCTGCA AAAAGATCCT GGAGTACCAC AGCCAGACAG ACGATTACTA CAACTCCTGC
    CTCATAGAAT TACGGGCCCA GATGAGGAGA TTTGAGGAGC TTCTGCCCCA GGTGTGTTGG
    CTGGTGATGG AGAATTTCAA GGAGCACCAT TGGAAAAAAT GTTGTGCTTC CACCAAAGAG
    ATCAGAGAAC AGTTCTTGAA TTACCAGGAA GAGCTGGAGA AAAGGAAGGA TGAAAATGCC
    CAGAAGCTCC ACCCAAATCT CGGACACCCT GCACAGCTCC AAGATATGGA GTCTCTATGT
    CAAGTGGAAG AGAAGAGGCA GGGAGATTTG GACGCCTTGA TTGTGGTAAC CAAGGAGAAG
    CTGGAGGAAT GCACAAGGAA GTACGCCCGG TTTTTCATAG CCACCTTGGC CACCTTCACC
    GAGAAGTTCC TGCTGCAGTT GGATGAGGTG GTCACCATTG ACGATGTCCA GGTTGCAAAG
    ATGGAGCCCC CCAAACAGAA AACTTCAATC CTAATACGGA GGAAACTCAC CGGGCTGTCC
    CTGGAGGAAG AGAGTGAGAA GCCCCTGATT GAACGGGGGA GCAGAAAGTG GCCAGGAATC
    AAACCTACTG AAGTCACCAT CCAAAACAAG ATTCTCCTCC GGAAAACACC ATGTATCACA
    ACCACCAAAA CAACACTGGG CCACCTGGCA TCTGTGGAAG CCCGAGATGC TGTCTACCTG
    AAATATTTGG CATCATTTGA AGAGGAGCTG AAGAAGGTCC AGGAGCACAC CGCACTGCAG
    GTGAAAGAGG CTCAGCGCTG GAAGGACAGC TGGAAACACT CCGTGAACAT CATCCAGGGC
    CTGTACTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91290
    Clone ID Related Accession (Same CDS sequence) XM_015472553.1 , XM_015464564.1
    Accession Version XM_015464564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATCCCAA AGGACAGCAG GAAGCCCTTG GTGAAGAAGG TGGATGTCTG TGAAGGCGAG 
    ATGATGTCTC CTCGGCAGCG GAAGTGGATG CGCAGCCTAC CCAATGACTG GACCACAGAA
    AACCCTGTTC TTTACAGGGA AAAGCAAATA GCCAAAAAGG AAAAAGCTCA AGAGAGTGAG
    AACGCCATCG ACGCCAGAGA AGTCCGGGGC CTCACGGACA CCATTGTTCC TGAGAGGATC
    AGCAGCACCA CCTTTCAAAA GCAAGGAGAT TCCAAGAGGA GGGGCTACGA GAGTGCCCTG
    GCGAGCTTTA AGGAGGAGAT CGCTCAGATT GGGATGGAAA TGGAACCTCT CATCTTGGAA
    CCAGGAGCAC TTCTTTTAAA AAAGCTGGCT GAGTCTGATG AGGATGTGAA CAATCTCTTC
    AAAAAGGTGG AAAACGACAG CAACCTCGAA GACTACACCA TCCAAACCCT GTTGGAGCTG
    AGGGATCAGG TGGCCGAGAA GTTCCTGCTG CAGAAGCAGG AGATCAAGGA GCTGGATGAG
    ACACTGCACT CACTTGAGTT CTCCCGGGTA GATAAACTAA AAAGCGTGTT GAAGAAATAT
    GTGGAAATCA TAGAGAAAAC TTCCTACCTC ATGCAGCCCG ACGTGTATAG GCTGATCAAT
    AAAGAAGCCA TGATCATAAA CCACGCCCTG CTGGGCAACC GGAGGGCTCT CGCCAAGCTG
    TTCGTCAACC TGATGGAAGC CAGGCTGCAG CAGGAGCTTG ATAGCCACCG CCGCTGGCAG
    GGCCTGATGG ATGCCTGGAA GGCTCTCAAG AAGGAGTATC TGGTGCAGGA TTTCAGTGAG
    TTCATGGCCA GTGAGAGAAT CCAGACTCCT CCGGCTGTGA AGAAAGAGCT TGAGACCATG
    ATGAAGAACC AGAGCAGCCT GCAGCAAAAG CGACTGGACC ATCTGTGCAC CATCTGTGAC
    CTCCTGCCCC CCAATTACAG CAAAGCTCAG CTAACGGAAT GGCGTTCTTC TCTCGACTCG
    TTGAACAAGC ACATCGATGC CTACCACATG GACTGCATGA TGCGGATCCG CTTGCAGTAC
    GAGAAGATCT GGCAGGAGTG CCTAGCCCAA GTGCAGAAGT GCAGGAAGCA GCTGCTGGAC
    TGGAAGGCCT TCACTGAGGA GGAGGCAGAG AGCCTGGTGA GCCCATCCTT CTTCCAGATG
    GTGGGATGTC TCCAGAGCAA GGTGGAGGAG GAGCTGGAGG TCTTGGACAA ATCCTTCGAG
    ACCGTGGCAA AGCAGACAGA GCAGCAGAGC TCAGACCTCT TCAGCTACTT CCAGGAGGCC
    GTGAACCTAT GGGAGACACA TCAGAGTGTG CTGTTGATGC AGGAGATGGA GCTCGAGAAG
    AGGATGGAGC AGCAGCGGCA GAAGCACAGG CGGGAGAACC AGGCCCAGGA GGCTCATCTT
    GACAAGCTCT TGGACCAACT GAGGCAGCAA AGCTATGAGA ATAACCTGAA GTCACACCTG
    GAAAAGGCCA AGGATTTTCT GAAGAATATG CAATGCAGGT ATGAGGCTTT TCATGGCCTC
    CTGACAAAGG AAGTGATGGA GTACCCAGTG ATCATACTGA AGGAACTCAA CTCCTACAGC
    TCCCACCTCA GCCGACACTT CTGTGTTCGT GAAATCTTTG AACAGAACTT GGAGGGAGAA
    GTCATTTTCC AATTGAGGGA ACCAGAGCCA CATGAAAAAC AGTTCCAGAA GAGAATGAGA
    AGACCGAGAG GAAAACGAAG GTCCAAAGCA AACGCCCGCA AAGGTGACGA AAGCAACAGT
    GGGAGAACGA GTTCCCTCAA GCCAGCCGAA GAGATAGAAG AAGAAGAAGA TCAAGAGGCC
    GAGTCCTCCA CCACACAAGA GGTGGCCGTG AGCCCACAGG AGAAGTCCGT ACTGAGTGAA
    GAGATGGATG AATCCAGGGA GGACTCTATT TCAGGACTGG AAGAAATACA GGTTGAAAGA
    GACAGCTCCT TGAGATCACC CCCAAACCAG GCCAGTGTGA TAGTTCACGA AGAAGAAGAG
    GAGGAGAAGA AGGAAAAGGT GGAGGAGGAG AGGGAGGAGG AGGAGGTGGA AGAGGAGGAG
    GAGGAAGAGG AGGAGGAGAA GGAGGAAGAG GAGGAGGAGC GGAGGAGCTT GTCTGTGGAT
    GAGGTCAAAG CCCAGGAGGA GAGTCTAGGG GAGATCTCCC AGGAGGAGAT GGAGTTCTTC
    ACAACCTCCA GTGGGAACAC TTACTTTGTT TTCCTACCCC TGAAACAAGA AGAGAACAGC
    AAGACACCCC ACTCCAACCT TCCTGCCACT CTCTTCAAGG ACATTTCCAG TGCCAGGGTC
    TTAGAAGAAG TGATCATTCC ATCCAGACTA GTTTTAGAGA TTAAGAAACA GCTTCGAACT
    GGCTTTTTTG AGCACTTGGA GAAATGGTTT GACCAATGTG CCCTCAATGC TCGGGTCATC
    GTGGCCAGCA AGATTGATGA GCTGGATTCA GAACTGGAGC TGCGTCTGCA CCTGCACCAG
    CCAAGAGCCC AGCACGTTGA GAAGGACATC CACAATGTCA GAGCAGCTGA GCTCTTGCTT
    CATCAAGAAC GGCTGAACAG CCACTGTGCA GGGGTGATGG AGACACTGAG GAAGGAGAGG
    ATGATGTTCT GCCAGTTCCA AGAAGAGCAA AACTTGAAGA GCAAACACTT CCGCCGCAAG
    ATTTCTGACA TGGAGCACAT CTTCTTGAAC GCCACCAAGA GCCAGAAGCT GGCGACTCTC
    AGCAGCACTC TGCAGCGGGA GCTGCTCAGC TACGTCGACG TCATCCAGGT GTCCCTGCGC
    AGCTTCCGCC AGTACCTGGA GGAGAGCATG GGCAAGCTGC GCTACACCAA CATTGAGTTC
    ATCAAGCACT GCAGATTGTT TTCAGAGGGA GGCAACTTCT CTTCTGAAGA AATTGACTCA
    CTGTGTCATC GACTAGAGAA GGAAGCTGCC CGGATAGAGT TTGTTGAAAA TTTAATCATG
    ATCAACATGG AAAAGATGGA GAGTGATTAC CTGGACCAGG CTAATGAGTT CATCAGCAAG
    TTTGAAAGTA AATTCCATAA CCTGTCTGTG GACCTTATTT TCATAGAGAA AATCCAGCGC
    TTGCTGACAA ATTTGCAAGT AAACCTCAAG TGCGAGGTGG CAAAATCCAA TTTGCAGACA
    GAGGGATTAA ATTCTTCTCT AGAACAGCTT CAAAGCAAGG TAGAAATGTG CCGAAGCTTA
    AAAAGGAATA AACAAGTAGT GAGCACAGAA GACCTCCTTG GCCTTATTCG AACTTGGAAA
    GAAAAACTGA GCCAAAGGAT TGAGTATCTC AACTGCAAGC TGGACATGTT ATCCATGACT
    CACCTGGCCT TTCCTGACAA CGTCTTGACT GACCTGGAGG TAGATAGTGA CATCTTGTTA
    TCTTCAGAAG TCTATGAAGA AGAGGCTAAG GTAGACATGG TCACCCCTGA GTCCTTTGCC
    CAGCCAAGCC GCATGGGGAA GTCCATGATT GAAGACCCAG CTGTGGAGGT GGTCAAGAGG
    ATCCTGCAGT TTCCTGACTC AAAGTCCTCA ACCCTTCAGT GTGACAAAGA ACGCTCCCAG
    ACAGCCTTGA GGCGACTCCG GAACCGAGCA GAGCACTCCT TGAAGAAGGA CTTGTCCATC
    GCCAGTGCCA CCTCGGTAGC AAGCGTCACG CGGTACACCA AGCCCAACCG ACTGGATAGA
    AAGTACCAGG TGCTCGGTGA CAAGCCTCCT CCAGCAGAGG ATTTTAAAGG CATCGTCCTG
    AGCCTGCTCT GGGAGAGCAA CGAGCACCTG CTGGCGGTCG CGGAGGAGTT TTACCGCAAA
    GAGAAACGCT CAGTCACCAG GCCCGACTGC ATGTACGAAA CCTTCGACCA GTGCGCGGAG
    AACATCTGCA AAAAGATCCT GGAGTACCAC AGCCAGACAG ACGATTACTA CAACTCCTGC
    CTCATAGAAT TACGGGCCCA GATGAGGAGA TTTGAGGAGC TTCTGCCCCA GGTGTGTTGG
    CTGGTGATGG AGAATTTCAA GGAGCACCAT TGGAAAAAAT GTTGTGCTTC CACCAAAGAG
    ATCAGAGAAC AGTTCTTGAA TTACCAGGAA GAGCTGGAGA AAAGGAAGGA TGAAAATGCC
    CAGAAGCTCC ACCCAAATCT CGGACACCCT GCACAGCTCC AAGATATGGA GTCTCTATGT
    CAAGTGGAAG AGAAGAGGCA GGGAGATTTG GACGCCTTGA TTGTGGTAAC CAAGGAGAAG
    CTGGAGGAAT GCACAAGGAA GTACGCCCGG TTTTTCATAG CCACCTTGGC CACCTTCACC
    GAGAAGTTCC TGCTGCAGTT GGATGAGGTG GTCACCATTG ACGATGTCCA GGTTGCAAAG
    ATGGAGCCCC CCAAACAGAA AACTTCAATC CTAATACGGA GGAAACTCAC CGGGCTGTCC
    CTGGAGGAAG AGAGTGAGAA GCCCCTGATT GAACGGGGGA GCAGAAAGTG GCCAGGAATC
    AAACCTACTG AAGTCACCAT CCAAAACAAG ATTCTCCTCC GGAAAACACC ATGTATCACA
    ACCACCAAAA CAACACTGGG CCACCTGGCA TCTGTGGAAG CCCGAGATGC TGTCTACCTG
    AAATATTTGG CATCATTTGA AGAGGAGCTG AAGAAGGTCC AGGAGCACAC CGCACTGCAG
    GTGAAAGAGG CTCAGCGCTG GAAGGACAGC TGGAAACACT CCGTGAACAT CATCCAGGGC
    CTGTACTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320050.1
    CDS637..5388
    Translation

    Target ORF information:

    RefSeq Version XM_015464564.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATCCCAA AGGACAGCAG GAAGCCCTTG GTGAAGAAGG TGGATGTCTG TGAAGGCGAG 
    ATGATGTCTC CTCGGCAGCG GAAGTGGATG CGCAGCCTAC CCAATGACTG GACCACAGAA
    AACCCTGTTC TTTACAGGGA AAAGCAAATA GCCAAAAAGG AAAAAGCTCA AGAGAGTGAG
    AACGCCATCG ACGCCAGAGA AGTCCGGGGC CTCACGGACA CCATTGTTCC TGAGAGGATC
    AGCAGCACCA CCTTTCAAAA GCAAGGAGAT TCCAAGAGGA GGGGCTACGA GAGTGCCCTG
    GCGAGCTTTA AGGAGGAGAT CGCTCAGATT GGGATGGAAA TGGAACCTCT CATCTTGGAA
    CCAGGAGCAC TTCTTTTAAA AAAGCTGGCT GAGTCTGATG AGGATGTGAA CAATCTCTTC
    AAAAAGGTGG AAAACGACAG CAACCTCGAA GACTACACCA TCCAAACCCT GTTGGAGCTG
    AGGGATCAGG TGGCCGAGAA GTTCCTGCTG CAGAAGCAGG AGATCAAGGA GCTGGATGAG
    ACACTGCACT CACTTGAGTT CTCCCGGGTA GATAAACTAA AAAGCGTGTT GAAGAAATAT
    GTGGAAATCA TAGAGAAAAC TTCCTACCTC ATGCAGCCCG ACGTGTATAG GCTGATCAAT
    AAAGAAGCCA TGATCATAAA CCACGCCCTG CTGGGCAACC GGAGGGCTCT CGCCAAGCTG
    TTCGTCAACC TGATGGAAGC CAGGCTGCAG CAGGAGCTTG ATAGCCACCG CCGCTGGCAG
    GGCCTGATGG ATGCCTGGAA GGCTCTCAAG AAGGAGTATC TGGTGCAGGA TTTCAGTGAG
    TTCATGGCCA GTGAGAGAAT CCAGACTCCT CCGGCTGTGA AGAAAGAGCT TGAGACCATG
    ATGAAGAACC AGAGCAGCCT GCAGCAAAAG CGACTGGACC ATCTGTGCAC CATCTGTGAC
    CTCCTGCCCC CCAATTACAG CAAAGCTCAG CTAACGGAAT GGCGTTCTTC TCTCGACTCG
    TTGAACAAGC ACATCGATGC CTACCACATG GACTGCATGA TGCGGATCCG CTTGCAGTAC
    GAGAAGATCT GGCAGGAGTG CCTAGCCCAA GTGCAGAAGT GCAGGAAGCA GCTGCTGGAC
    TGGAAGGCCT TCACTGAGGA GGAGGCAGAG AGCCTGGTGA GCCCATCCTT CTTCCAGATG
    GTGGGATGTC TCCAGAGCAA GGTGGAGGAG GAGCTGGAGG TCTTGGACAA ATCCTTCGAG
    ACCGTGGCAA AGCAGACAGA GCAGCAGAGC TCAGACCTCT TCAGCTACTT CCAGGAGGCC
    GTGAACCTAT GGGAGACACA TCAGAGTGTG CTGTTGATGC AGGAGATGGA GCTCGAGAAG
    AGGATGGAGC AGCAGCGGCA GAAGCACAGG CGGGAGAACC AGGCCCAGGA GGCTCATCTT
    GACAAGCTCT TGGACCAACT GAGGCAGCAA AGCTATGAGA ATAACCTGAA GTCACACCTG
    GAAAAGGCCA AGGATTTTCT GAAGAATATG CAATGCAGGT ATGAGGCTTT TCATGGCCTC
    CTGACAAAGG AAGTGATGGA GTACCCAGTG ATCATACTGA AGGAACTCAA CTCCTACAGC
    TCCCACCTCA GCCGACACTT CTGTGTTCGT GAAATCTTTG AACAGAACTT GGAGGGAGAA
    GTCATTTTCC AATTGAGGGA ACCAGAGCCA CATGAAAAAC AGTTCCAGAA GAGAATGAGA
    AGACCGAGAG GAAAACGAAG GTCCAAAGCA AACGCCCGCA AAGGTGACGA AAGCAACAGT
    GGGAGAACGA GTTCCCTCAA GCCAGCCGAA GAGATAGAAG AAGAAGAAGA TCAAGAGGCC
    GAGTCCTCCA CCACACAAGA GGTGGCCGTG AGCCCACAGG AGAAGTCCGT ACTGAGTGAA
    GAGATGGATG AATCCAGGGA GGACTCTATT TCAGGACTGG AAGAAATACA GGTTGAAAGA
    GACAGCTCCT TGAGATCACC CCCAAACCAG GCCAGTGTGA TAGTTCACGA AGAAGAAGAG
    GAGGAGAAGA AGGAAAAGGT GGAGGAGGAG AGGGAGGAGG AGGAGGTGGA AGAGGAGGAG
    GAGGAAGAGG AGGAGGAGAA GGAGGAAGAG GAGGAGGAGC GGAGGAGCTT GTCTGTGGAT
    GAGGTCAAAG CCCAGGAGGA GAGTCTAGGG GAGATCTCCC AGGAGGAGAT GGAGTTCTTC
    ACAACCTCCA GTGGGAACAC TTACTTTGTT TTCCTACCCC TGAAACAAGA AGAGAACAGC
    AAGACACCCC ACTCCAACCT TCCTGCCACT CTCTTCAAGG ACATTTCCAG TGCCAGGGTC
    TTAGAAGAAG TGATCATTCC ATCCAGACTA GTTTTAGAGA TTAAGAAACA GCTTCGAACT
    GGCTTTTTTG AGCACTTGGA GAAATGGTTT GACCAATGTG CCCTCAATGC TCGGGTCATC
    GTGGCCAGCA AGATTGATGA GCTGGATTCA GAACTGGAGC TGCGTCTGCA CCTGCACCAG
    CCAAGAGCCC AGCACGTTGA GAAGGACATC CACAATGTCA GAGCAGCTGA GCTCTTGCTT
    CATCAAGAAC GGCTGAACAG CCACTGTGCA GGGGTGATGG AGACACTGAG GAAGGAGAGG
    ATGATGTTCT GCCAGTTCCA AGAAGAGCAA AACTTGAAGA GCAAACACTT CCGCCGCAAG
    ATTTCTGACA TGGAGCACAT CTTCTTGAAC GCCACCAAGA GCCAGAAGCT GGCGACTCTC
    AGCAGCACTC TGCAGCGGGA GCTGCTCAGC TACGTCGACG TCATCCAGGT GTCCCTGCGC
    AGCTTCCGCC AGTACCTGGA GGAGAGCATG GGCAAGCTGC GCTACACCAA CATTGAGTTC
    ATCAAGCACT GCAGATTGTT TTCAGAGGGA GGCAACTTCT CTTCTGAAGA AATTGACTCA
    CTGTGTCATC GACTAGAGAA GGAAGCTGCC CGGATAGAGT TTGTTGAAAA TTTAATCATG
    ATCAACATGG AAAAGATGGA GAGTGATTAC CTGGACCAGG CTAATGAGTT CATCAGCAAG
    TTTGAAAGTA AATTCCATAA CCTGTCTGTG GACCTTATTT TCATAGAGAA AATCCAGCGC
    TTGCTGACAA ATTTGCAAGT AAACCTCAAG TGCGAGGTGG CAAAATCCAA TTTGCAGACA
    GAGGGATTAA ATTCTTCTCT AGAACAGCTT CAAAGCAAGG TAGAAATGTG CCGAAGCTTA
    AAAAGGAATA AACAAGTAGT GAGCACAGAA GACCTCCTTG GCCTTATTCG AACTTGGAAA
    GAAAAACTGA GCCAAAGGAT TGAGTATCTC AACTGCAAGC TGGACATGTT ATCCATGACT
    CACCTGGCCT TTCCTGACAA CGTCTTGACT GACCTGGAGG TAGATAGTGA CATCTTGTTA
    TCTTCAGAAG TCTATGAAGA AGAGGCTAAG GTAGACATGG TCACCCCTGA GTCCTTTGCC
    CAGCCAAGCC GCATGGGGAA GTCCATGATT GAAGACCCAG CTGTGGAGGT GGTCAAGAGG
    ATCCTGCAGT TTCCTGACTC AAAGTCCTCA ACCCTTCAGT GTGACAAAGA ACGCTCCCAG
    ACAGCCTTGA GGCGACTCCG GAACCGAGCA GAGCACTCCT TGAAGAAGGA CTTGTCCATC
    GCCAGTGCCA CCTCGGTAGC AAGCGTCACG CGGTACACCA AGCCCAACCG ACTGGATAGA
    AAGTACCAGG TGCTCGGTGA CAAGCCTCCT CCAGCAGAGG ATTTTAAAGG CATCGTCCTG
    AGCCTGCTCT GGGAGAGCAA CGAGCACCTG CTGGCGGTCG CGGAGGAGTT TTACCGCAAA
    GAGAAACGCT CAGTCACCAG GCCCGACTGC ATGTACGAAA CCTTCGACCA GTGCGCGGAG
    AACATCTGCA AAAAGATCCT GGAGTACCAC AGCCAGACAG ACGATTACTA CAACTCCTGC
    CTCATAGAAT TACGGGCCCA GATGAGGAGA TTTGAGGAGC TTCTGCCCCA GGTGTGTTGG
    CTGGTGATGG AGAATTTCAA GGAGCACCAT TGGAAAAAAT GTTGTGCTTC CACCAAAGAG
    ATCAGAGAAC AGTTCTTGAA TTACCAGGAA GAGCTGGAGA AAAGGAAGGA TGAAAATGCC
    CAGAAGCTCC ACCCAAATCT CGGACACCCT GCACAGCTCC AAGATATGGA GTCTCTATGT
    CAAGTGGAAG AGAAGAGGCA GGGAGATTTG GACGCCTTGA TTGTGGTAAC CAAGGAGAAG
    CTGGAGGAAT GCACAAGGAA GTACGCCCGG TTTTTCATAG CCACCTTGGC CACCTTCACC
    GAGAAGTTCC TGCTGCAGTT GGATGAGGTG GTCACCATTG ACGATGTCCA GGTTGCAAAG
    ATGGAGCCCC CCAAACAGAA AACTTCAATC CTAATACGGA GGAAACTCAC CGGGCTGTCC
    CTGGAGGAAG AGAGTGAGAA GCCCCTGATT GAACGGGGGA GCAGAAAGTG GCCAGGAATC
    AAACCTACTG AAGTCACCAT CCAAAACAAG ATTCTCCTCC GGAAAACACC ATGTATCACA
    ACCACCAAAA CAACACTGGG CCACCTGGCA TCTGTGGAAG CCCGAGATGC TGTCTACCTG
    AAATATTTGG CATCATTTGA AGAGGAGCTG AAGAAGGTCC AGGAGCACAC CGCACTGCAG
    GTGAAAGAGG CTCAGCGCTG GAAGGACAGC TGGAAACACT CCGTGAACAT CATCCAGGGC
    CTGTACTTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91291
    Clone ID Related Accession (Same CDS sequence) XM_015464565.1 , XM_015472554.1
    Accession Version XM_015464565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC ACGTCACGCG GTACACCAAG CCCAACCGAC TGGATAGAAA GTACCAGGTG
    CTCGGTGACA AGCCTCCTCC AGCAGAGGAT TTTAAAGGCA TCGTCCTGAG CCTGCTCTGG
    GAGAGCAACG AGCACCTGCT GGCGGTCGCG GAGGAGTTTT ACCGCAAAGA GAAACGCTCA
    GTCACCAGGC CCGACTGCAT GTACGAAACC TTCGACCAGT GCGCGGAGAA CATCTGCAAA
    AAGATCCTGG AGTACCACAG CCAGACAGAC GATTACTACA ACTCCTGCCT CATAGAATTA
    CGGGCCCAGA TGAGGAGATT TGAGGAGCTT CTGCCCCAGG TGTGTTGGCT GGTGATGGAG
    AATTTCAAGG AGCACCATTG GAAAAAATGT TGTGCTTCCA CCAAAGAGAT CAGAGAACAG
    TTCTTGAATT ACCAGGAAGA GCTGGAGAAA AGGAAGGATG AAAATGCCCA GAAGCTCCAC
    CCAAATCTCG GACACCCTGC ACAGCTCCAA GATATGGAGT CTCTATGTCA AGTGGAAGAG
    AAGAGGCAGG GAGATTTGGA CGCCTTGATT GTGGTAACCA AGGAGAAGCT GGAGGAATGC
    ACAAGGAAGT ACGCCCGGTT TTTCATAGCC ACCTTGGCCA CCTTCACCGA GAAGTTCCTG
    CTGCAGTTGG ATGAGGTGGT CACCATTGAC GATGTCCAGG TTGCAAAGAT GGAGCCCCCC
    AAACAGAAAA CTTCAATCCT AATACGGAGG AAACTCACCG GGCTGTCCCT GGAGGAAGAG
    AGTGAGAAGC CCCTGATTGA ACGGGGGAGC AGAAAGTGGC CAGGAATCAA ACCTACTGAA
    GTCACCATCC AAAACAAGAT TCTCCTCCGG AAAACACCAT GTATCACAAC CACCAAAACA
    ACACTGGGCC ACCTGGCATC TGTGGAAGCC CGAGATGCTG TCTACCTGAA ATATTTGGCA
    TCATTTGAAG AGGAGCTGAA GAAGGTCCAG GAGCACACCG CACTGCAGGT GAAAGAGGCT
    CAGCGCTGGA AGGACAGCTG GAAACACTCC GTGAACATCA TCCAGGGCCT GTACTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320051.1
    CDS285..5024
    Translation

    Target ORF information:

    RefSeq Version XM_015464565.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC ACGTCACGCG GTACACCAAG CCCAACCGAC TGGATAGAAA GTACCAGGTG
    CTCGGTGACA AGCCTCCTCC AGCAGAGGAT TTTAAAGGCA TCGTCCTGAG CCTGCTCTGG
    GAGAGCAACG AGCACCTGCT GGCGGTCGCG GAGGAGTTTT ACCGCAAAGA GAAACGCTCA
    GTCACCAGGC CCGACTGCAT GTACGAAACC TTCGACCAGT GCGCGGAGAA CATCTGCAAA
    AAGATCCTGG AGTACCACAG CCAGACAGAC GATTACTACA ACTCCTGCCT CATAGAATTA
    CGGGCCCAGA TGAGGAGATT TGAGGAGCTT CTGCCCCAGG TGTGTTGGCT GGTGATGGAG
    AATTTCAAGG AGCACCATTG GAAAAAATGT TGTGCTTCCA CCAAAGAGAT CAGAGAACAG
    TTCTTGAATT ACCAGGAAGA GCTGGAGAAA AGGAAGGATG AAAATGCCCA GAAGCTCCAC
    CCAAATCTCG GACACCCTGC ACAGCTCCAA GATATGGAGT CTCTATGTCA AGTGGAAGAG
    AAGAGGCAGG GAGATTTGGA CGCCTTGATT GTGGTAACCA AGGAGAAGCT GGAGGAATGC
    ACAAGGAAGT ACGCCCGGTT TTTCATAGCC ACCTTGGCCA CCTTCACCGA GAAGTTCCTG
    CTGCAGTTGG ATGAGGTGGT CACCATTGAC GATGTCCAGG TTGCAAAGAT GGAGCCCCCC
    AAACAGAAAA CTTCAATCCT AATACGGAGG AAACTCACCG GGCTGTCCCT GGAGGAAGAG
    AGTGAGAAGC CCCTGATTGA ACGGGGGAGC AGAAAGTGGC CAGGAATCAA ACCTACTGAA
    GTCACCATCC AAAACAAGAT TCTCCTCCGG AAAACACCAT GTATCACAAC CACCAAAACA
    ACACTGGGCC ACCTGGCATC TGTGGAAGCC CGAGATGCTG TCTACCTGAA ATATTTGGCA
    TCATTTGAAG AGGAGCTGAA GAAGGTCCAG GAGCACACCG CACTGCAGGT GAAAGAGGCT
    CAGCGCTGGA AGGACAGCTG GAAACACTCC GTGAACATCA TCCAGGGCCT GTACTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91291
    Clone ID Related Accession (Same CDS sequence) XM_015464565.1 , XM_015472554.1
    Accession Version XM_015472554.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC ACGTCACGCG GTACACCAAG CCCAACCGAC TGGATAGAAA GTACCAGGTG
    CTCGGTGACA AGCCTCCTCC AGCAGAGGAT TTTAAAGGCA TCGTCCTGAG CCTGCTCTGG
    GAGAGCAACG AGCACCTGCT GGCGGTCGCG GAGGAGTTTT ACCGCAAAGA GAAACGCTCA
    GTCACCAGGC CCGACTGCAT GTACGAAACC TTCGACCAGT GCGCGGAGAA CATCTGCAAA
    AAGATCCTGG AGTACCACAG CCAGACAGAC GATTACTACA ACTCCTGCCT CATAGAATTA
    CGGGCCCAGA TGAGGAGATT TGAGGAGCTT CTGCCCCAGG TGTGTTGGCT GGTGATGGAG
    AATTTCAAGG AGCACCATTG GAAAAAATGT TGTGCTTCCA CCAAAGAGAT CAGAGAACAG
    TTCTTGAATT ACCAGGAAGA GCTGGAGAAA AGGAAGGATG AAAATGCCCA GAAGCTCCAC
    CCAAATCTCG GACACCCTGC ACAGCTCCAA GATATGGAGT CTCTATGTCA AGTGGAAGAG
    AAGAGGCAGG GAGATTTGGA CGCCTTGATT GTGGTAACCA AGGAGAAGCT GGAGGAATGC
    ACAAGGAAGT ACGCCCGGTT TTTCATAGCC ACCTTGGCCA CCTTCACCGA GAAGTTCCTG
    CTGCAGTTGG ATGAGGTGGT CACCATTGAC GATGTCCAGG TTGCAAAGAT GGAGCCCCCC
    AAACAGAAAA CTTCAATCCT AATACGGAGG AAACTCACCG GGCTGTCCCT GGAGGAAGAG
    AGTGAGAAGC CCCTGATTGA ACGGGGGAGC AGAAAGTGGC CAGGAATCAA ACCTACTGAA
    GTCACCATCC AAAACAAGAT TCTCCTCCGG AAAACACCAT GTATCACAAC CACCAAAACA
    ACACTGGGCC ACCTGGCATC TGTGGAAGCC CGAGATGCTG TCTACCTGAA ATATTTGGCA
    TCATTTGAAG AGGAGCTGAA GAAGGTCCAG GAGCACACCG CACTGCAGGT GAAAGAGGCT
    CAGCGCTGGA AGGACAGCTG GAAACACTCC GTGAACATCA TCCAGGGCCT GTACTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328040.1
    CDS285..5024
    Translation

    Target ORF information:

    RefSeq Version XM_015472554.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472554.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC ACGTCACGCG GTACACCAAG CCCAACCGAC TGGATAGAAA GTACCAGGTG
    CTCGGTGACA AGCCTCCTCC AGCAGAGGAT TTTAAAGGCA TCGTCCTGAG CCTGCTCTGG
    GAGAGCAACG AGCACCTGCT GGCGGTCGCG GAGGAGTTTT ACCGCAAAGA GAAACGCTCA
    GTCACCAGGC CCGACTGCAT GTACGAAACC TTCGACCAGT GCGCGGAGAA CATCTGCAAA
    AAGATCCTGG AGTACCACAG CCAGACAGAC GATTACTACA ACTCCTGCCT CATAGAATTA
    CGGGCCCAGA TGAGGAGATT TGAGGAGCTT CTGCCCCAGG TGTGTTGGCT GGTGATGGAG
    AATTTCAAGG AGCACCATTG GAAAAAATGT TGTGCTTCCA CCAAAGAGAT CAGAGAACAG
    TTCTTGAATT ACCAGGAAGA GCTGGAGAAA AGGAAGGATG AAAATGCCCA GAAGCTCCAC
    CCAAATCTCG GACACCCTGC ACAGCTCCAA GATATGGAGT CTCTATGTCA AGTGGAAGAG
    AAGAGGCAGG GAGATTTGGA CGCCTTGATT GTGGTAACCA AGGAGAAGCT GGAGGAATGC
    ACAAGGAAGT ACGCCCGGTT TTTCATAGCC ACCTTGGCCA CCTTCACCGA GAAGTTCCTG
    CTGCAGTTGG ATGAGGTGGT CACCATTGAC GATGTCCAGG TTGCAAAGAT GGAGCCCCCC
    AAACAGAAAA CTTCAATCCT AATACGGAGG AAACTCACCG GGCTGTCCCT GGAGGAAGAG
    AGTGAGAAGC CCCTGATTGA ACGGGGGAGC AGAAAGTGGC CAGGAATCAA ACCTACTGAA
    GTCACCATCC AAAACAAGAT TCTCCTCCGG AAAACACCAT GTATCACAAC CACCAAAACA
    ACACTGGGCC ACCTGGCATC TGTGGAAGCC CGAGATGCTG TCTACCTGAA ATATTTGGCA
    TCATTTGAAG AGGAGCTGAA GAAGGTCCAG GAGCACACCG CACTGCAGGT GAAAGAGGCT
    CAGCGCTGGA AGGACAGCTG GAAACACTCC GTGAACATCA TCCAGGGCCT GTACTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91292
    Clone ID Related Accession (Same CDS sequence) XM_015472555.1 , XM_015464566.1
    Accession Version XM_015472555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AGATGGAGCC CCCCAAACAG AAAACTTCAA TCCTAATACG GAGGAAACTC
    ACCGGGCTGT CCCTGGAGGA AGAGAGTGAG AAGCCCCTGA TTGAACGGGG GAGCAGAAAG
    TGGCCAGGAA TCAAACCTAC TGAAGTCACC ATCCAAAACA AGATTCTCCT CCGGAAAACA
    CCATGTATCA CAACCACCAA AACAACACTG GGCCACCTGG CATCTGTGGA AGCCCGAGAT
    GCTGTCTACC TGAAATATTT GGCATCATTT GAAGAGGAGC TGAAGAAGGT CCAGGAGCAC
    ACCGCACTGC AGGTGAAAGA GGCTCAGCGC TGGAAGGACA GCTGGAAACA CTCCGTGAAC
    ATCATCCAGG GCCTGTACTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328041.1
    CDS285..4748
    Translation

    Target ORF information:

    RefSeq Version XM_015472555.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AGATGGAGCC CCCCAAACAG AAAACTTCAA TCCTAATACG GAGGAAACTC
    ACCGGGCTGT CCCTGGAGGA AGAGAGTGAG AAGCCCCTGA TTGAACGGGG GAGCAGAAAG
    TGGCCAGGAA TCAAACCTAC TGAAGTCACC ATCCAAAACA AGATTCTCCT CCGGAAAACA
    CCATGTATCA CAACCACCAA AACAACACTG GGCCACCTGG CATCTGTGGA AGCCCGAGAT
    GCTGTCTACC TGAAATATTT GGCATCATTT GAAGAGGAGC TGAAGAAGGT CCAGGAGCAC
    ACCGCACTGC AGGTGAAAGA GGCTCAGCGC TGGAAGGACA GCTGGAAACA CTCCGTGAAC
    ATCATCCAGG GCCTGTACTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91292
    Clone ID Related Accession (Same CDS sequence) XM_015472555.1 , XM_015464566.1
    Accession Version XM_015464566.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AGATGGAGCC CCCCAAACAG AAAACTTCAA TCCTAATACG GAGGAAACTC
    ACCGGGCTGT CCCTGGAGGA AGAGAGTGAG AAGCCCCTGA TTGAACGGGG GAGCAGAAAG
    TGGCCAGGAA TCAAACCTAC TGAAGTCACC ATCCAAAACA AGATTCTCCT CCGGAAAACA
    CCATGTATCA CAACCACCAA AACAACACTG GGCCACCTGG CATCTGTGGA AGCCCGAGAT
    GCTGTCTACC TGAAATATTT GGCATCATTT GAAGAGGAGC TGAAGAAGGT CCAGGAGCAC
    ACCGCACTGC AGGTGAAAGA GGCTCAGCGC TGGAAGGACA GCTGGAAACA CTCCGTGAAC
    ATCATCCAGG GCCTGTACTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320052.1
    CDS285..4748
    Translation

    Target ORF information:

    RefSeq Version XM_015464566.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG AGATGGAGCC CCCCAAACAG AAAACTTCAA TCCTAATACG GAGGAAACTC
    ACCGGGCTGT CCCTGGAGGA AGAGAGTGAG AAGCCCCTGA TTGAACGGGG GAGCAGAAAG
    TGGCCAGGAA TCAAACCTAC TGAAGTCACC ATCCAAAACA AGATTCTCCT CCGGAAAACA
    CCATGTATCA CAACCACCAA AACAACACTG GGCCACCTGG CATCTGTGGA AGCCCGAGAT
    GCTGTCTACC TGAAATATTT GGCATCATTT GAAGAGGAGC TGAAGAAGGT CCAGGAGCAC
    ACCGCACTGC AGGTGAAAGA GGCTCAGCGC TGGAAGGACA GCTGGAAACA CTCCGTGAAC
    ATCATCCAGG GCCTGTACTT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91293
    Clone ID Related Accession (Same CDS sequence) XM_015464567.1 , XM_015472556.1
    Accession Version XM_015464567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG GACCCCTTTT GATGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320053.1
    CDS285..4394
    Translation

    Target ORF information:

    RefSeq Version XM_015464567.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG GACCCCTTTT GATGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91293
    Clone ID Related Accession (Same CDS sequence) XM_015464567.1 , XM_015472556.1
    Accession Version XM_015472556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product coiled-coil domain-containing protein 180 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104105.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG GACCCCTTTT GATGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328042.1
    CDS285..4394
    Translation

    Target ORF information:

    RefSeq Version XM_015472556.1
    Organism Bos taurus(cattle)
    Definition Bos taurus coiled-coil domain containing 180 (CCDC180), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTCCG TGTGGAAGGT GACCCAGATT CCCAGCGGGA AGGTCTACCA GCAGATCTTC 
    GAGGCTGAGG TGCAGCTGGT CCGCTCCCTG GCATCCTCTA GGAAGCGAGC TGTGGAGCGT
    TCCATGATCC CAAAGGACAG CAGGAAGCCC TTGGTGAAGA AGGTGGATGT CTGTGAAGGC
    GAGATGATGT CTCCTCGGCA GCGGAAGTGG ATGCGCAGCC TACCCAATGA CTGGACCACA
    GAAAACCCTG TTCTTTACAG GGAAAAGCAA ATAGCCAAAA AGGAAAAAGC TCAAGAGAGT
    GAGAACGCCA TCGACGCCAG AGAAGTCCGG GGCCTCACGG ACACCATTGT TCCTGAGAGG
    ATCAGCAGCA CCACCTTTCA AAAGCAAGGA GATTCCAAGA GGAGGGGCTA CGAGAGTGCC
    CTGGCGAGCT TTAAGGAGGA GATCGCTCAG ATTGGGATGG AAATGGAACC TCTCATCTTG
    GAACCAGGAG CACTTCTTTT AAAAAAGCTG GCTGAGTCTG ATGAGGATGT GAACAATCTC
    TTCAAAAAGG TGGAAAACGA CAGCAACCTC GAAGACTACA CCATCCAAAC CCTGTTGGAG
    CTGAGGGATC AGGTGGCCGA GAAGTTCCTG CTGCAGAAGC AGGAGATCAA GGAGCTGGAT
    GAGACACTGC ACTCACTTGA GTTCTCCCGG GTAGATAAAC TAAAAAGCGT GTTGAAGAAA
    TATGTGGAAA TCATAGAGAA AACTTCCTAC CTCATGCAGC CCGACGTGTA TAGGCTGATC
    AATAAAGAAG CCATGATCAT AAACCACGCC CTGCTGGGCA ACCGGAGGGC TCTCGCCAAG
    CTGTTCGTCA ACCTGATGGA AGCCAGGCTG CAGCAGGAGC TTGATAGCCA CCGCCGCTGG
    CAGGGCCTGA TGGATGCCTG GAAGGCTCTC AAGAAGGAGT ATCTGGTGCA GGATTTCAGT
    GAGTTCATGG CCAGTGAGAG AATCCAGACT CCTCCGGCTG TGAAGAAAGA GCTTGAGACC
    ATGATGAAGA ACCAGAGCAG CCTGCAGCAA AAGCGACTGG ACCATCTGTG CACCATCTGT
    GACCTCCTGC CCCCCAATTA CAGCAAAGCT CAGCTAACGG AATGGCGTTC TTCTCTCGAC
    TCGTTGAACA AGCACATCGA TGCCTACCAC ATGGACTGCA TGATGCGGAT CCGCTTGCAG
    TACGAGAAGA TCTGGCAGGA GTGCCTAGCC CAAGTGCAGA AGTGCAGGAA GCAGCTGCTG
    GACTGGAAGG CCTTCACTGA GGAGGAGGCA GAGAGCCTGG TGAGCCCATC CTTCTTCCAG
    ATGGTGGGAT GTCTCCAGAG CAAGGTGGAG GAGGAGCTGG AGGTCTTGGA CAAATCCTTC
    GAGACCGTGG CAAAGCAGAC AGAGCAGCAG AGCTCAGACC TCTTCAGCTA CTTCCAGGAG
    GCCGTGAACC TATGGGAGAC ACATCAGAGT GTGCTGTTGA TGCAGGAGAT GGAGCTCGAG
    AAGAGGATGG AGCAGCAGCG GCAGAAGCAC AGGCGGGAGA ACCAGGCCCA GGAGGCTCAT
    CTTGACAAGC TCTTGGACCA ACTGAGGCAG CAAAGCTATG AGAATAACCT GAAGTCACAC
    CTGGAAAAGG CCAAGGATTT TCTGAAGAAT ATGCAATGCA GGTATGAGGC TTTTCATGGC
    CTCCTGACAA AGGAAGTGAT GGAGTACCCA GTGATCATAC TGAAGGAACT CAACTCCTAC
    AGCTCCCACC TCAGCCGACA CTTCTGTGTT CGTGAAATCT TTGAACAGAA CTTGGAGGGA
    GAAGTCATTT TCCAATTGAG GGAACCAGAG CCACATGAAA AACAGTTCCA GAAGAGAATG
    AGAAGACCGA GAGGAAAACG AAGGTCCAAA GCAAACGCCC GCAAAGGTGA CGAAAGCAAC
    AGTGGGAGAA CGAGTTCCCT CAAGCCAGCC GAAGAGATAG AAGAAGAAGA AGATCAAGAG
    GCCGAGTCCT CCACCACACA AGAGGTGGCC GTGAGCCCAC AGGAGAAGTC CGTACTGAGT
    GAAGAGATGG ATGAATCCAG GGAGGACTCT ATTTCAGGAC TGGAAGAAAT ACAGGTTGAA
    AGAGACAGCT CCTTGAGATC ACCCCCAAAC CAGGCCAGTG TGATAGTTCA CGAAGAAGAA
    GAGGAGGAGA AGAAGGAAAA GGTGGAGGAG GAGAGGGAGG AGGAGGAGGT GGAAGAGGAG
    GAGGAGGAAG AGGAGGAGGA GAAGGAGGAA GAGGAGGAGG AGCGGAGGAG CTTGTCTGTG
    GATGAGGTCA AAGCCCAGGA GGAGAGTCTA GGGGAGATCT CCCAGGAGGA GATGGAGTTC
    TTCACAACCT CCAGTGGGAA CACTTACTTT GTTTTCCTAC CCCTGAAACA AGAAGAGAAC
    AGCAAGACAC CCCACTCCAA CCTTCCTGCC ACTCTCTTCA AGGACATTTC CAGTGCCAGG
    GTCTTAGAAG AAGTGATCAT TCCATCCAGA CTAGTTTTAG AGATTAAGAA ACAGCTTCGA
    ACTGGCTTTT TTGAGCACTT GGAGAAATGG TTTGACCAAT GTGCCCTCAA TGCTCGGGTC
    ATCGTGGCCA GCAAGATTGA TGAGCTGGAT TCAGAACTGG AGCTGCGTCT GCACCTGCAC
    CAGCCAAGAG CCCAGCACGT TGAGAAGGAC ATCCACAATG TCAGAGCAGC TGAGCTCTTG
    CTTCATCAAG AACGGCTGAA CAGCCACTGT GCAGGGGTGA TGGAGACACT GAGGAAGGAG
    AGGATGATGT TCTGCCAGTT CCAAGAAGAG CAAAACTTGA AGAGCAAACA CTTCCGCCGC
    AAGATTTCTG ACATGGAGCA CATCTTCTTG AACGCCACCA AGAGCCAGAA GCTGGCGACT
    CTCAGCAGCA CTCTGCAGCG GGAGCTGCTC AGCTACGTCG ACGTCATCCA GGTGTCCCTG
    CGCAGCTTCC GCCAGTACCT GGAGGAGAGC ATGGGCAAGC TGCGCTACAC CAACATTGAG
    TTCATCAAGC ACTGCAGATT GTTTTCAGAG GGAGGCAACT TCTCTTCTGA AGAAATTGAC
    TCACTGTGTC ATCGACTAGA GAAGGAAGCT GCCCGGATAG AGTTTGTTGA AAATTTAATC
    ATGATCAACA TGGAAAAGAT GGAGAGTGAT TACCTGGACC AGGCTAATGA GTTCATCAGC
    AAGTTTGAAA GTAAATTCCA TAACCTGTCT GTGGACCTTA TTTTCATAGA GAAAATCCAG
    CGCTTGCTGA CAAATTTGCA AGTAAACCTC AAGTGCGAGG TGGCAAAATC CAATTTGCAG
    ACAGAGGGAT TAAATTCTTC TCTAGAACAG CTTCAAAGCA AGGTAGAAAT GTGCCGAAGC
    TTAAAAAGGA ATAAACAAGT AGTGAGCACA GAAGACCTCC TTGGCCTTAT TCGAACTTGG
    AAAGAAAAAC TGAGCCAAAG GATTGAGTAT CTCAACTGCA AGCTGGACAT GTTATCCATG
    ACTCACCTGG CCTTTCCTGA CAACGTCTTG ACTGACCTGG AGGTAGATAG TGACATCTTG
    TTATCTTCAG AAGTCTATGA AGAAGAGGCT AAGGTAGACA TGGTCACCCC TGAGTCCTTT
    GCCCAGCCAA GCCGCATGGG GAAGTCCATG ATTGAAGACC CAGCTGTGGA GGTGGTCAAG
    AGGATCCTGC AGTTTCCTGA CTCAAAGTCC TCAACCCTTC AGTGTGACAA AGAACGCTCC
    CAGACAGCCT TGAGGCGACT CCGGAACCGA GCAGAGCACT CCTTGAAGAA GGACTTGTCC
    ATCGCCAGTG CCACCTCGGT AGCAAGCGTC ACGCGGTACA CCAAGCCCAA CCGACTGGAT
    AGAAAGTACC AGGTGCTCGG TGACAAGCCT CCTCCAGCAG AGGATTTTAA AGGCATCGTC
    CTGAGCCTGC TCTGGGAGAG CAACGAGCAC CTGCTGGCGG TCGCGGAGGA GTTTTACCGC
    AAAGAGAAAC GCTCAGTCAC CAGGCCCGAC TGCATGTACG AAACCTTCGA CCAGTGCGCG
    GAGAACATCT GCAAAAAGAT CCTGGAGTAC CACAGCCAGA CAGACGATTA CTACAACTCC
    TGCCTCATAG GACCCCTTTT GATGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.