CACNA1S cDNA ORF clone, Bos taurus(cattle)

The following CACNA1S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223792 XM_024976574.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa223793 XM_024976575.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa93088 XM_015466951.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa93088 XM_015475322.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223792
    Clone ID Related Accession (Same CDS sequence) XM_024976574.1
    Accession Version XM_024976574.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5619bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
    CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG
    CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC
    ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC
    AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG
    GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC
    GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG
    CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC
    GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC
    AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC
    CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG
    AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG
    TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC
    TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG
    GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG
    CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC
    TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA
    ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC
    TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG
    ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG
    ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG
    ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC
    TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG
    CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GGATCTTCAA GATCACCAAG
    TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC
    TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC
    TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC
    CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG
    TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC
    TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT
    GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG
    CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG
    GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG
    AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC
    TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG
    CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC
    ATCTTCAGCC CCACCAACAA GATCCGCGTC CTGTGCCACC GCATTGTCAA TGCCACCTGG
    TTCACTAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCTTGGC CGCCGAGGAC
    CCCCTCCGGG CTGAGTCCGT GAGGAACCAG ATCCTCGGGT ATTTTGACAT CGGGTTCACC
    TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGGGCCTT CCTGCACAAG
    GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGG TGGTGGTGGC CGTGTCCCTC
    ATCTCCATGG GACTCGAGTC CAGCACCATC TCCGTGGTGA AGATCCTGAG GGTCCTGAGG
    GTGCTCCGAC CGCTCCGGGC CATCAACAGA GCCAAAGGCC TGAAGCACGT GGTACAGTGT
    GTGTTCGTGG CCATCCGCAC CATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC
    ATGTTCGCTT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTG
    TCCAAGATGA CGGAGGAGGA GTGCAGGGGC TACTACTATG TGTACAAAGA TGGAGACCCC
    ACACAGATAG AGGTGCGTCC CCGCCAGTGG GTGCACAACG CCTTCCACTT CGACAACGTG
    CTTTCGGCCA TGATGTCCCT CTTCACCGTG TCCACCTTCG AGGGATGGCC TGAGCTGCTG
    TACAAGGCCA TTGACTCTCA TGAGGAGGAC AAGGGCCCCG TGTACAACCA CCGCGTGGAG
    ATGGCCATTT TCTTCATCAT CTACATCATC CTTATCGCCT TCTTCATGAT GAACATCTTC
    GTGGGCTTTG TCATCGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG
    CTGGACAAGA ACCAGCGCCA GTGCGTGCAG TACGCCCTGA AGGCCCGGCC CCTGAGGTGC
    TACATCCCCA AGAACCCCTA CCAGTACCGG GTGTGGTACA TCGTCACCTC CTCCTACTTC
    GAGTACCTGA TGTTCGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC
    AACCAGTCGG AGCAAATGAA CCACATCTCT GACATCCTCA ACGTAGCCTT CACCATCATC
    TTCACACTGG AGATGGTCCT CAAGCTCATG GCGTTCAAGG CCAGGGGCTA TTTTGGGGAC
    CCCTGGAACG TGTTTGACTT TCTGATTGTC ATCGGCAGCA TCATCGACGT CATCCTCAGT
    GAGATCGACA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG CGGCTGCGGG
    AACGTTGACC CGGACGAGAG CGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGTGTC
    ATGAGGCTGA TCAAGCTGCT GAGCCGCGCG GAGGGCGTGC GCACGCTGCT CTGGACTTTC
    ATCAAGTCCT TCCAGGCCCT GCCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC
    TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCT TGGTGGACGG GACCCAGATC
    AACCGGAACA ACAACTTCCA GACCTTCCCC CAGGCGGTGC TGCTGCTCTT CAGGTGCGCC
    ACGGGGGAGG CGTGGCAGGA GATCCTGCTG GCCTGCAGCT ACGGCCAGCT GTGTGACCCC
    GAGTCGGACT ACGCCCCCGG GGAGGAGTAC ACCTGTGGCA CGGACTTCGC CTACTACTAC
    TTCCTCAGCT TCTACATGCT GTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCCGTCATC
    ATGGACAACT TTGACTACCT GACACGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT
    GAGTTCAAGG CCATCTGGGC GGAGTACGAC CCGGAGGCTA CGGGCAGGAT CAAGCACCTG
    GACGTGGTGA CCCTGCTGAG GAGGATCCAG CCCCCGCTGG GCTTCGGGAA GTTCTGCCCC
    CACCGGGTAG CGTGTAAGCG GCTGGTGGGC ATGAACATGC CCCTCAACAG TGACGGCACG
    GTCACCTTCA ACGCCACGCT CTTCGCCCTG GTGCGCACAG CTCTGAAGAT CAAGACGGAA
    GGTAACTTCG AGCAGGCCAA CGAGGAGCTG CGAGCCATCA TCAAGAAGGT CTGGAAGAGG
    ACCAGCATGA AGCTCCTGGA CCAGGTCATC CCTCCCATAG GAGATGACGA GGTGACGGTG
    GGGAAGTTCT ACGCCACGTT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAGGCGCCAG
    GAGGAGTATT ATGGGTACCG GCCCAAGAAG GACACCGTGC AGATCCAGGC TGGACTGAGG
    ACCATCGAGG AAGAGGCAGC CCCTGAGATC CGCCGCACCA TCTCGGGAGA CCTGATGGCC
    GAAGAGGAGC TGGAGAGAGC CATGGTGGAG GCCGCGATGG AGGAAGGGAT CTTCCGGAGG
    ACCGGCGGCC TGTTTGGCCA GGTGGACAGC TTCCTGGAAA GGACCAACTC GTTGGCCCCG
    CACATGGCCA ACCAGAGACC CCTCCAGTTT ACTGAAATTG AGATGGAGGA GATGGAATCG
    CCCGTCTTCC TGGAGGACTT CCCTCAACGT CCGAGTGCCC ACCCCCTTGC TCGTGCCAAC
    ACCAACAACG CCAACGCCAA CGTCGCCAGT GGCAACGGCA ACCATGGCAA CAGCCCAGCA
    TTTCCCAGTG TCCACTACGA AGGGGAGCTC GCAGGGGAGA CAGAGACGCC TGCCACCAGA
    GGAGGACTCT TCGGCCAGCC CCGGGGGGCC CTGGGGCCCC ACAGCAAGTC CCGAGTAGAG
    TGGCCGCAGG GACAGCTGCC CCGGAGAAGG ACGCCCAGGG CCCAGGCCCC CGCTGCCTCC
    TGCCAGCACG CGGGAGCAGA GTCCCAGGTG CCCGAGGAGA GGAGCCCCAC CCCAGGGTCC
    CTGCCCGGGG AGGCGCCGCC CGGCAGTAGG ACAGAGGACG GAACCCCTGG GGGTCCAGCT
    CCAGCCATGG CCCTGCTGAT CCGCGAGGCT CTGGTTCGAG GGGGCCTGGA CAGCTTGGCG
    GCGGATGCTA ACTTCATCAT GGCCACGGGC CAGGCGCTGG CAGAGGCCTG CCGGATGGAA
    CCGCGGGAAG TGGAGGTGGC AGCCGCAGAG CTTCTGCAGG GACGCGAGGC CCTGGAGGGC
    GTGCCTGGTG CCCGAGGGTG CCCAGGCCTC GGGTCCTCCC TGGGCAGCCT CGACCTGCGT
    GAGGGCTCCC AGGAGACCCT GATTCCCCGC AGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832342.1
    CDS94..5712
    Translation

    Target ORF information:

    RefSeq Version XM_024976574.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
    CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG
    CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC
    ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC
    AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG
    GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC
    GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG
    CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC
    GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC
    AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC
    CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG
    AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG
    TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC
    TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG
    GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG
    CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC
    TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA
    ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC
    TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG
    ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG
    ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG
    ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC
    TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG
    CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GGATCTTCAA GATCACCAAG
    TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC
    TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC
    TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC
    CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG
    TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC
    TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT
    GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG
    CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG
    GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG
    AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC
    TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG
    CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC
    ATCTTCAGCC CCACCAACAA GATCCGCGTC CTGTGCCACC GCATTGTCAA TGCCACCTGG
    TTCACTAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCTTGGC CGCCGAGGAC
    CCCCTCCGGG CTGAGTCCGT GAGGAACCAG ATCCTCGGGT ATTTTGACAT CGGGTTCACC
    TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGGGCCTT CCTGCACAAG
    GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGG TGGTGGTGGC CGTGTCCCTC
    ATCTCCATGG GACTCGAGTC CAGCACCATC TCCGTGGTGA AGATCCTGAG GGTCCTGAGG
    GTGCTCCGAC CGCTCCGGGC CATCAACAGA GCCAAAGGCC TGAAGCACGT GGTACAGTGT
    GTGTTCGTGG CCATCCGCAC CATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC
    ATGTTCGCTT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTG
    TCCAAGATGA CGGAGGAGGA GTGCAGGGGC TACTACTATG TGTACAAAGA TGGAGACCCC
    ACACAGATAG AGGTGCGTCC CCGCCAGTGG GTGCACAACG CCTTCCACTT CGACAACGTG
    CTTTCGGCCA TGATGTCCCT CTTCACCGTG TCCACCTTCG AGGGATGGCC TGAGCTGCTG
    TACAAGGCCA TTGACTCTCA TGAGGAGGAC AAGGGCCCCG TGTACAACCA CCGCGTGGAG
    ATGGCCATTT TCTTCATCAT CTACATCATC CTTATCGCCT TCTTCATGAT GAACATCTTC
    GTGGGCTTTG TCATCGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG
    CTGGACAAGA ACCAGCGCCA GTGCGTGCAG TACGCCCTGA AGGCCCGGCC CCTGAGGTGC
    TACATCCCCA AGAACCCCTA CCAGTACCGG GTGTGGTACA TCGTCACCTC CTCCTACTTC
    GAGTACCTGA TGTTCGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC
    AACCAGTCGG AGCAAATGAA CCACATCTCT GACATCCTCA ACGTAGCCTT CACCATCATC
    TTCACACTGG AGATGGTCCT CAAGCTCATG GCGTTCAAGG CCAGGGGCTA TTTTGGGGAC
    CCCTGGAACG TGTTTGACTT TCTGATTGTC ATCGGCAGCA TCATCGACGT CATCCTCAGT
    GAGATCGACA CTTTCCTGGC CTCCAGCGGG GGACTGTATT GCCTGGGTGG CGGCTGCGGG
    AACGTTGACC CGGACGAGAG CGCCCGCATC TCCAGCGCCT TCTTCCGCCT GTTCCGTGTC
    ATGAGGCTGA TCAAGCTGCT GAGCCGCGCG GAGGGCGTGC GCACGCTGCT CTGGACTTTC
    ATCAAGTCCT TCCAGGCCCT GCCCTACGTG GCTCTGCTCA TCGTCATGCT CTTCTTCATC
    TACGCGGTCA TCGGCATGCA GATGTTCGGG AAGATCGCCT TGGTGGACGG GACCCAGATC
    AACCGGAACA ACAACTTCCA GACCTTCCCC CAGGCGGTGC TGCTGCTCTT CAGGTGCGCC
    ACGGGGGAGG CGTGGCAGGA GATCCTGCTG GCCTGCAGCT ACGGCCAGCT GTGTGACCCC
    GAGTCGGACT ACGCCCCCGG GGAGGAGTAC ACCTGTGGCA CGGACTTCGC CTACTACTAC
    TTCCTCAGCT TCTACATGCT GTGCGCCTTC CTGATCATCA ACCTCTTTGT GGCCGTCATC
    ATGGACAACT TTGACTACCT GACACGGGAC TGGTCCATCC TGGGCCCTCA TCACCTGGAT
    GAGTTCAAGG CCATCTGGGC GGAGTACGAC CCGGAGGCTA CGGGCAGGAT CAAGCACCTG
    GACGTGGTGA CCCTGCTGAG GAGGATCCAG CCCCCGCTGG GCTTCGGGAA GTTCTGCCCC
    CACCGGGTAG CGTGTAAGCG GCTGGTGGGC ATGAACATGC CCCTCAACAG TGACGGCACG
    GTCACCTTCA ACGCCACGCT CTTCGCCCTG GTGCGCACAG CTCTGAAGAT CAAGACGGAA
    GGTAACTTCG AGCAGGCCAA CGAGGAGCTG CGAGCCATCA TCAAGAAGGT CTGGAAGAGG
    ACCAGCATGA AGCTCCTGGA CCAGGTCATC CCTCCCATAG GAGATGACGA GGTGACGGTG
    GGGAAGTTCT ACGCCACGTT CCTCATCCAG GAGCACTTCC GGAAGTTCAT GAGGCGCCAG
    GAGGAGTATT ATGGGTACCG GCCCAAGAAG GACACCGTGC AGATCCAGGC TGGACTGAGG
    ACCATCGAGG AAGAGGCAGC CCCTGAGATC CGCCGCACCA TCTCGGGAGA CCTGATGGCC
    GAAGAGGAGC TGGAGAGAGC CATGGTGGAG GCCGCGATGG AGGAAGGGAT CTTCCGGAGG
    ACCGGCGGCC TGTTTGGCCA GGTGGACAGC TTCCTGGAAA GGACCAACTC GTTGGCCCCG
    CACATGGCCA ACCAGAGACC CCTCCAGTTT ACTGAAATTG AGATGGAGGA GATGGAATCG
    CCCGTCTTCC TGGAGGACTT CCCTCAACGT CCGAGTGCCC ACCCCCTTGC TCGTGCCAAC
    ACCAACAACG CCAACGCCAA CGTCGCCAGT GGCAACGGCA ACCATGGCAA CAGCCCAGCA
    TTTCCCAGTG TCCACTACGA AGGGGAGCTC GCAGGGGAGA CAGAGACGCC TGCCACCAGA
    GGAGGACTCT TCGGCCAGCC CCGGGGGGCC CTGGGGCCCC ACAGCAAGTC CCGAGTAGAG
    TGGCCGCAGG GACAGCTGCC CCGGAGAAGG ACGCCCAGGG CCCAGGCCCC CGCTGCCTCC
    TGCCAGCACG CGGGAGCAGA GTCCCAGGTG CCCGAGGAGA GGAGCCCCAC CCCAGGGTCC
    CTGCCCGGGG AGGCGCCGCC CGGCAGTAGG ACAGAGGACG GAACCCCTGG GGGTCCAGCT
    CCAGCCATGG CCCTGCTGAT CCGCGAGGCT CTGGTTCGAG GGGGCCTGGA CAGCTTGGCG
    GCGGATGCTA ACTTCATCAT GGCCACGGGC CAGGCGCTGG CAGAGGCCTG CCGGATGGAA
    CCGCGGGAAG TGGAGGTGGC AGCCGCAGAG CTTCTGCAGG GACGCGAGGC CCTGGAGGGC
    GTGCCTGGTG CCCGAGGGTG CCCAGGCCTC GGGTCCTCCC TGGGCAGCCT CGACCTGCGT
    GAGGGCTCCC AGGAGACCCT GATTCCCCGC AGGCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223793
    Clone ID Related Accession (Same CDS sequence) XM_024976575.1
    Accession Version XM_024976575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5562bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1S isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
    CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG
    CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC
    ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC
    AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG
    GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC
    GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG
    CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC
    GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC
    AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC
    CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG
    AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG
    TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC
    TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG
    GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG
    CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC
    TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA
    ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC
    TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG
    ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG
    ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG
    ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC
    TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG
    CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GGATCTTCAA GATCACCAAG
    TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC
    TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC
    TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC
    CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG
    TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC
    TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT
    GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG
    CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG
    GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG
    AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC
    TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG
    CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC
    ATCTTCAGCC CCACCAACAA GATCCGCGTC CTGTGCCACC GCATTGTCAA TGCCACCTGG
    TTCACTAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCTTGGC CGCCGAGGAC
    CCCCTCCGGG CTGAGTCCGT GAGGAACCAG ATCCTCGGGT ATTTTGACAT CGGGTTCACC
    TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGGGCCTT CCTGCACAAG
    GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGG TGGTGGTGGC CGTGTCCCTC
    ATCTCCATGG GACTCGAGTC CAGCACCATC TCCGTGGTGA AGATCCTGAG GGTCCTGAGG
    GTGCTCCGAC CGCTCCGGGC CATCAACAGA GCCAAAGGCC TGAAGCACGT GGTACAGTGT
    GTGTTCGTGG CCATCCGCAC CATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC
    ATGTTCGCTT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTG
    TCCAAGATGA CGGAGGAGGA GTGCAGGGGC TACTACTATG TGTACAAAGA TGGAGACCCC
    ACACAGATAG AGGTGCGTCC CCGCCAGTGG GTGCACAACG CCTTCCACTT CGACAACGTG
    CTTTCGGCCA TGATGTCCCT CTTCACCGTG TCCACCTTCG AGGGATGGCC TGAGCTGCTG
    TACAAGGCCA TTGACTCTCA TGAGGAGGAC AAGGGCCCCG TGTACAACCA CCGCGTGGAG
    ATGGCCATTT TCTTCATCAT CTACATCATC CTTATCGCCT TCTTCATGAT GAACATCTTC
    GTGGGCTTTG TCATCGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG
    CTGGACAAGA ACCAGCGCCA GTGCGTGCAG TACGCCCTGA AGGCCCGGCC CCTGAGGTGC
    TACATCCCCA AGAACCCCTA CCAGTACCGG GTGTGGTACA TCGTCACCTC CTCCTACTTC
    GAGTACCTGA TGTTCGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC
    AACCAGTCGG AGCAAATGAA CCACATCTCT GACATCCTCA ACGTAGCCTT CACCATCATC
    TTCACACTGG AGATGGTCCT CAAGCTCATG GCGTTCAAGG CCAGGGGCTA TTTTGGGGAC
    CCCTGGAACG TGTTTGACTT TCTGATTGTC ATCGGCAGCA TCATCGACGT CATCCTCAGT
    GAGATCGACG ACCCGGACGA GAGCGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT
    GTCATGAGGC TGATCAAGCT GCTGAGCCGC GCGGAGGGCG TGCGCACGCT GCTCTGGACT
    TTCATCAAGT CCTTCCAGGC CCTGCCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC
    ATCTACGCGG TCATCGGCAT GCAGATGTTC GGGAAGATCG CCTTGGTGGA CGGGACCCAG
    ATCAACCGGA ACAACAACTT CCAGACCTTC CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC
    GCCACGGGGG AGGCGTGGCA GGAGATCCTG CTGGCCTGCA GCTACGGCCA GCTGTGTGAC
    CCCGAGTCGG ACTACGCCCC CGGGGAGGAG TACACCTGTG GCACGGACTT CGCCTACTAC
    TACTTCCTCA GCTTCTACAT GCTGTGCGCC TTCCTGATCA TCAACCTCTT TGTGGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACACGG GACTGGTCCA TCCTGGGCCC TCATCACCTG
    GATGAGTTCA AGGCCATCTG GGCGGAGTAC GACCCGGAGG CTACGGGCAG GATCAAGCAC
    CTGGACGTGG TGACCCTGCT GAGGAGGATC CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC
    CCCCACCGGG TAGCGTGTAA GCGGCTGGTG GGCATGAACA TGCCCCTCAA CAGTGACGGC
    ACGGTCACCT TCAACGCCAC GCTCTTCGCC CTGGTGCGCA CAGCTCTGAA GATCAAGACG
    GAAGGTAACT TCGAGCAGGC CAACGAGGAG CTGCGAGCCA TCATCAAGAA GGTCTGGAAG
    AGGACCAGCA TGAAGCTCCT GGACCAGGTC ATCCCTCCCA TAGGAGATGA CGAGGTGACG
    GTGGGGAAGT TCTACGCCAC GTTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAGGCGC
    CAGGAGGAGT ATTATGGGTA CCGGCCCAAG AAGGACACCG TGCAGATCCA GGCTGGACTG
    AGGACCATCG AGGAAGAGGC AGCCCCTGAG ATCCGCCGCA CCATCTCGGG AGACCTGATG
    GCCGAAGAGG AGCTGGAGAG AGCCATGGTG GAGGCCGCGA TGGAGGAAGG GATCTTCCGG
    AGGACCGGCG GCCTGTTTGG CCAGGTGGAC AGCTTCCTGG AAAGGACCAA CTCGTTGGCC
    CCGCACATGG CCAACCAGAG ACCCCTCCAG TTTACTGAAA TTGAGATGGA GGAGATGGAA
    TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA CGTCCGAGTG CCCACCCCCT TGCTCGTGCC
    AACACCAACA ACGCCAACGC CAACGTCGCC AGTGGCAACG GCAACCATGG CAACAGCCCA
    GCATTTCCCA GTGTCCACTA CGAAGGGGAG CTCGCAGGGG AGACAGAGAC GCCTGCCACC
    AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG GCCCTGGGGC CCCACAGCAA GTCCCGAGTA
    GAGTGGCCGC AGGGACAGCT GCCCCGGAGA AGGACGCCCA GGGCCCAGGC CCCCGCTGCC
    TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG GTGCCCGAGG AGAGGAGCCC CACCCCAGGG
    TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT AGGACAGAGG ACGGAACCCC TGGGGGTCCA
    GCTCCAGCCA TGGCCCTGCT GATCCGCGAG GCTCTGGTTC GAGGGGGCCT GGACAGCTTG
    GCGGCGGATG CTAACTTCAT CATGGCCACG GGCCAGGCGC TGGCAGAGGC CTGCCGGATG
    GAACCGCGGG AAGTGGAGGT GGCAGCCGCA GAGCTTCTGC AGGGACGCGA GGCCCTGGAG
    GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC CTCGGGTCCT CCCTGGGCAG CCTCGACCTG
    CGTGAGGGCT CCCAGGAGAC CCTGATTCCC CGCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832343.1
    CDS94..5655
    Translation

    Target ORF information:

    RefSeq Version XM_024976575.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 S (CACNA1S), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
    CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG
    CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC
    ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC
    AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG
    GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC
    GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG
    CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC
    GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC
    AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC
    CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG
    AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG
    TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC
    TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG
    GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG
    CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC
    TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA
    ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC
    TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG
    ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG
    ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG
    ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC
    TGCTTCGTGG TGTGCAGCGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG
    CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GGATCTTCAA GATCACCAAG
    TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC
    TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC
    TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC
    CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG
    TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC
    TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT
    GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG
    CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG
    GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG
    AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC
    TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG
    CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC
    ATCTTCAGCC CCACCAACAA GATCCGCGTC CTGTGCCACC GCATTGTCAA TGCCACCTGG
    TTCACTAACT TCATCCTGCT CTTCATCCTG CTCAGCAGTG CCGCCTTGGC CGCCGAGGAC
    CCCCTCCGGG CTGAGTCCGT GAGGAACCAG ATCCTCGGGT ATTTTGACAT CGGGTTCACC
    TCTGTCTTCA CTGTGGAGAT TGTCCTCAAG ATGACCACCT ACGGGGCCTT CCTGCACAAG
    GGCTCCTTCT GCCGCAACTA CTTCAACATC CTGGACCTGG TGGTGGTGGC CGTGTCCCTC
    ATCTCCATGG GACTCGAGTC CAGCACCATC TCCGTGGTGA AGATCCTGAG GGTCCTGAGG
    GTGCTCCGAC CGCTCCGGGC CATCAACAGA GCCAAAGGCC TGAAGCACGT GGTACAGTGT
    GTGTTCGTGG CCATCCGCAC CATCGGGAAC ATCGTCCTGG TCACCACCCT CCTGCAGTTC
    ATGTTCGCTT GCATCGGCGT CCAGCTCTTC AAGGGGAAGT TCTTCAGCTG CAATGACTTG
    TCCAAGATGA CGGAGGAGGA GTGCAGGGGC TACTACTATG TGTACAAAGA TGGAGACCCC
    ACACAGATAG AGGTGCGTCC CCGCCAGTGG GTGCACAACG CCTTCCACTT CGACAACGTG
    CTTTCGGCCA TGATGTCCCT CTTCACCGTG TCCACCTTCG AGGGATGGCC TGAGCTGCTG
    TACAAGGCCA TTGACTCTCA TGAGGAGGAC AAGGGCCCCG TGTACAACCA CCGCGTGGAG
    ATGGCCATTT TCTTCATCAT CTACATCATC CTTATCGCCT TCTTCATGAT GAACATCTTC
    GTGGGCTTTG TCATCGTCAC CTTCCAGGAG CAGGGGGAGA CTGAGTACAA GAACTGTGAG
    CTGGACAAGA ACCAGCGCCA GTGCGTGCAG TACGCCCTGA AGGCCCGGCC CCTGAGGTGC
    TACATCCCCA AGAACCCCTA CCAGTACCGG GTGTGGTACA TCGTCACCTC CTCCTACTTC
    GAGTACCTGA TGTTCGCCCT CATCATGCTC AACACCATCT GCCTGGGCAT GCAGCACTAC
    AACCAGTCGG AGCAAATGAA CCACATCTCT GACATCCTCA ACGTAGCCTT CACCATCATC
    TTCACACTGG AGATGGTCCT CAAGCTCATG GCGTTCAAGG CCAGGGGCTA TTTTGGGGAC
    CCCTGGAACG TGTTTGACTT TCTGATTGTC ATCGGCAGCA TCATCGACGT CATCCTCAGT
    GAGATCGACG ACCCGGACGA GAGCGCCCGC ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT
    GTCATGAGGC TGATCAAGCT GCTGAGCCGC GCGGAGGGCG TGCGCACGCT GCTCTGGACT
    TTCATCAAGT CCTTCCAGGC CCTGCCCTAC GTGGCTCTGC TCATCGTCAT GCTCTTCTTC
    ATCTACGCGG TCATCGGCAT GCAGATGTTC GGGAAGATCG CCTTGGTGGA CGGGACCCAG
    ATCAACCGGA ACAACAACTT CCAGACCTTC CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC
    GCCACGGGGG AGGCGTGGCA GGAGATCCTG CTGGCCTGCA GCTACGGCCA GCTGTGTGAC
    CCCGAGTCGG ACTACGCCCC CGGGGAGGAG TACACCTGTG GCACGGACTT CGCCTACTAC
    TACTTCCTCA GCTTCTACAT GCTGTGCGCC TTCCTGATCA TCAACCTCTT TGTGGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACACGG GACTGGTCCA TCCTGGGCCC TCATCACCTG
    GATGAGTTCA AGGCCATCTG GGCGGAGTAC GACCCGGAGG CTACGGGCAG GATCAAGCAC
    CTGGACGTGG TGACCCTGCT GAGGAGGATC CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC
    CCCCACCGGG TAGCGTGTAA GCGGCTGGTG GGCATGAACA TGCCCCTCAA CAGTGACGGC
    ACGGTCACCT TCAACGCCAC GCTCTTCGCC CTGGTGCGCA CAGCTCTGAA GATCAAGACG
    GAAGGTAACT TCGAGCAGGC CAACGAGGAG CTGCGAGCCA TCATCAAGAA GGTCTGGAAG
    AGGACCAGCA TGAAGCTCCT GGACCAGGTC ATCCCTCCCA TAGGAGATGA CGAGGTGACG
    GTGGGGAAGT TCTACGCCAC GTTCCTCATC CAGGAGCACT TCCGGAAGTT CATGAGGCGC
    CAGGAGGAGT ATTATGGGTA CCGGCCCAAG AAGGACACCG TGCAGATCCA GGCTGGACTG
    AGGACCATCG AGGAAGAGGC AGCCCCTGAG ATCCGCCGCA CCATCTCGGG AGACCTGATG
    GCCGAAGAGG AGCTGGAGAG AGCCATGGTG GAGGCCGCGA TGGAGGAAGG GATCTTCCGG
    AGGACCGGCG GCCTGTTTGG CCAGGTGGAC AGCTTCCTGG AAAGGACCAA CTCGTTGGCC
    CCGCACATGG CCAACCAGAG ACCCCTCCAG TTTACTGAAA TTGAGATGGA GGAGATGGAA
    TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA CGTCCGAGTG CCCACCCCCT TGCTCGTGCC
    AACACCAACA ACGCCAACGC CAACGTCGCC AGTGGCAACG GCAACCATGG CAACAGCCCA
    GCATTTCCCA GTGTCCACTA CGAAGGGGAG CTCGCAGGGG AGACAGAGAC GCCTGCCACC
    AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG GCCCTGGGGC CCCACAGCAA GTCCCGAGTA
    GAGTGGCCGC AGGGACAGCT GCCCCGGAGA AGGACGCCCA GGGCCCAGGC CCCCGCTGCC
    TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG GTGCCCGAGG AGAGGAGCCC CACCCCAGGG
    TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT AGGACAGAGG ACGGAACCCC TGGGGGTCCA
    GCTCCAGCCA TGGCCCTGCT GATCCGCGAG GCTCTGGTTC GAGGGGGCCT GGACAGCTTG
    GCGGCGGATG CTAACTTCAT CATGGCCACG GGCCAGGCGC TGGCAGAGGC CTGCCGGATG
    GAACCGCGGG AAGTGGAGGT GGCAGCCGCA GAGCTTCTGC AGGGACGCGA GGCCCTGGAG
    GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC CTCGGGTCCT CCCTGGGCAG CCTCGACCTG
    CGTGAGGGCT CCCAGGAGAC CCTGATTCCC CGCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93088
    Clone ID Related Accession (Same CDS sequence) XM_015475322.1 , XM_015466951.1
    Accession Version XM_015475322.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1S
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104441.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
    CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG
    CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC
    ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC
    AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG
    GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC
    GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG
    CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC
    GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC
    AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC
    CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG
    AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG
    TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC
    TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG
    GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG
    CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC
    TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA
    ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC
    TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG
    ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG
    ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG
    ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC
    TGCTTCGTGG TGTGCAGTGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG
    CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GCATCTTCAA GATCACCAAG
    TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC
    TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC
    TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC
    CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG
    TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC
    TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT
    GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG
    CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG
    GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG
    AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC
    TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG
    CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC
    ATCTTCAGCC CCACCAACAA GTGGGTGGCT CCTCGGCGGT GGGGCAGGGT GATGGTGGGG
    TCTCAGAGAA GGCTGCTTCC TGCTCCGAGA AGACCGCCTT CTCCCAACTC TCTGGGCCTA
    CAGTTACACT GTGAGAAAGG CATTCCTTTA ACTTCAGATC TCTTGCTCCA TTTAACGTGC
    CAGCATCCCC AGCAGACGTA TATTTTAAGC CTCACGCGTT ACTTATACCC AAGCGTGACC
    AGATACCTCC TGCTCTGCCA CCAGATCCTC GGGTATTTTG ACATCGGGTT CACCTCTGTC
    TTCACTGTGG AGATTGTCCT CAAGATGACC ACCTACGGGG CCTTCCTGCA CAAGGGCTCC
    TTTTGCCCAA ATGAAACATT CCGTGGGCAC CCAGTGTCTG TCTCCCTCCT TCCCTCCGGG
    ATACAGGGGA AGTTCTTCAG CTGCAATGAC TTGTCCAAGA TGACGGAGGA GGAGTGCAGG
    GGCTACTACT ATGTGTACAA AGATGGAGAC CCCACACAGA TAGAGGTGCG TCCCCGCCAG
    TGGGTGCACA ACGCCTTCCA CTTCGACAAC GTGCTTTCGG CCATGATGTC CCTCTTCACC
    GTGTCCACCT TCGAGGGATG GCCTGAGCTG CTGTACAAGG CCATTGACTC TCATGAGGAG
    GACAAGGGCC CCGTGTACAA CCACCGCGTG GAGATGGCCA TTTTCTTCAT CATCTACATC
    ATCCTTATCG CCTTCTTCAT GATGAACATC TTCGTGGGCT TTGTCATCGT CACCTTCCAG
    GAGCAGGGGG AGACTGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGCG CCAGTGCGTG
    CAGTACGCCC TGAAGGCCCG GCCCCTGAGG TGCTACATCC CCAAGAACCC CTACCAGTAC
    CGGGTGTGGT ACATCGTCAC CTCCTCCTAC TTCGAGTACC TGATGTTCGC CCTCATCATG
    CTCAACACCA TCTGCCTGGG CATGCAGCAC TACAACCAGT CGGAGCAAAT GAACCACATC
    TCTGACATCC TCAACGTAGC CTTCACCATC ATCTTCACAC TGGAGATGGT CCTCAAGCTC
    ATGGCGTTCA AGGCCAGGGG CTATTTTGGG GACCCCTGGA ACGTGTTTGA CTTTCTGATT
    GTCATCGGCA GCATCATCGA CGTCATCCTC AGTGAGATCG ACACTTTCCT GGCCTCCAGC
    GGGGGACTGT ATTGCCTGGG TGGCGGCTGC GGGAACGTTG ACCCGGACGA GAGCGCCCGC
    ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT GTCATGAGGC TGATCAAGCT GCTGAGCCGC
    GCGGAGGGCG TGCGCACGCT GCTCTGGACT TTCCATCCTC GCCCGCAGGC CCTGCCCTAC
    GTGGCTCTGC TCATCGTCAT GCTCTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC
    GGGAAGATCG CCTTGGTGGA CGGGACCCAG ATCAACCGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC GCCACGGGGG AGGCGTGGCA GGAGATCCTG
    CTGGCCTGCA GCTACGGCCA GCTGTGTGAC CCCGAGTCGG ACTACGCCCC CGGGGAGGAG
    TACACCTGTG GCACGGACTT CGCCTACTAC TACTTCCTCA GCTTCTACAT GCTGTGCGCC
    TTCCTGATCA TCAACCTCTT TGTGGCCGTC ATCATGGACA ACTTTGACTA CCTGACACGG
    GACTGGTCCA TCCTGGGCCC TCATCACCTG GATGAGTTCA AGGCCATCTG GGCGGAGTAC
    GACCCGGAGG CTACGGGCAG GATCAAGCAC CTGGACGTGG TGACCCTGCT GAGGAGGATC
    CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC CCCCACCGGG TAGCGTGTAA GCGGCTGGTG
    GGCATGAACA TGCCCCTCAA CAGTGACGGC ACGGTCACCT TCAACGCCAC GCTCTTCGCC
    CTGGTGCGCA CAGCTCTGAA GATCAAGACG GAAGGTAACT TCGAGCAGGC CAACGAGGAG
    CTGCGAGCCA TCATCAAGAA GGTCTGGAAG AGGACCAGCA TGAAGCTCCT GGACCAGGTC
    ATCCCTCCCA TAGGAGATGA CGAGGTGACG GTGGGGAAGT TCTACGCCAC GTTCCTCATC
    CAGGAGCACT TCCGGAAGTT CATGAGGCGC CAGGAGGAGT ATTATGGGTA CCGGCCCAAG
    AAGGACACCG TGCAGATCCA GGCTGGACTG AGGACCATCG AGGAAGAGGC AGCCCCTGAG
    ATCCGCCGCA CCATCTCGGG AGACCTGATG GCCGAAGAGG AGCTGGAGAG AGCCATGGTG
    GAGGCCGCGA TGGAGGAAGG GATCTTCCGG AGGACCGGCG GCCTGTTTGG CCAGGTGGAC
    AGCTTCCTGG AAAGGACCAA CTCGTTGGCC CCGCACATGG CCAACCAGAG ACCCCTCCAG
    TTTACTGAAA TTGAGATGGA GGAGATGGAA TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA
    CGTCCGAGTG CCCACCCCCT TGCTCGTGCC AACACCAACA ACGCCAACGC CAACGTCGCC
    AGTGGCAACG GCAACCATGG CAACAGCCCA GCATTTCCCA GTGTCCACTA CGAAGGGGAG
    CTCGCAGGGG AGACAGAGAC GCCTGCCACC AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG
    GCCCTGGGGC CCCACAGCAA GTCCCGAGTA GAGTGGCCGC AGGGACAGCT GCCCCGGAGA
    AGGACGCCCA GGGCCCAGGC CCCCGCTGCC TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG
    GTGCCCGAGG AGAGGAGCCC CACCCCAGGG TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT
    AGGACAGAGG ACGGAACCCC TGGGGGTCCA GCTCCAGCCA TGGCCCTGCT GATCCGCGAG
    GCTCTGGTTC AAGGGGGCCT GGACAGCTTG GCGGCGGATG CTAACTTCAT CATGGCCACG
    GGCCAGGCGC TGGCAGAGGC CTGCCGGATG GAACCGCGGG AAGTGGAGGT GGCAGCCGCA
    GAGCTTCTGC AGGGACGCGA GGCCCTGGAG GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC
    CTCGGGTCCT CCCTGGGCAG CCTCGACCTG CGTGAGGGCT CCCAGGAGAC CCTGATTCCC
    CGCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330808.1
    CDS95..5626
    Translation

    Target ORF information:

    RefSeq Version XM_015475322.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475322.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
    CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG
    CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC
    ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC
    AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG
    GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC
    GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG
    CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC
    GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC
    AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC
    CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG
    AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG
    TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC
    TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG
    GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG
    CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC
    TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA
    ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC
    TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG
    ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG
    ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG
    ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC
    TGCTTCGTGG TGTGCAGTGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG
    CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GCATCTTCAA GATCACCAAG
    TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC
    TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC
    TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC
    CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG
    TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC
    TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT
    GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG
    CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG
    GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG
    AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC
    TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG
    CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC
    ATCTTCAGCC CCACCAACAA GTGGGTGGCT CCTCGGCGGT GGGGCAGGGT GATGGTGGGG
    TCTCAGAGAA GGCTGCTTCC TGCTCCGAGA AGACCGCCTT CTCCCAACTC TCTGGGCCTA
    CAGTTACACT GTGAGAAAGG CATTCCTTTA ACTTCAGATC TCTTGCTCCA TTTAACGTGC
    CAGCATCCCC AGCAGACGTA TATTTTAAGC CTCACGCGTT ACTTATACCC AAGCGTGACC
    AGATACCTCC TGCTCTGCCA CCAGATCCTC GGGTATTTTG ACATCGGGTT CACCTCTGTC
    TTCACTGTGG AGATTGTCCT CAAGATGACC ACCTACGGGG CCTTCCTGCA CAAGGGCTCC
    TTTTGCCCAA ATGAAACATT CCGTGGGCAC CCAGTGTCTG TCTCCCTCCT TCCCTCCGGG
    ATACAGGGGA AGTTCTTCAG CTGCAATGAC TTGTCCAAGA TGACGGAGGA GGAGTGCAGG
    GGCTACTACT ATGTGTACAA AGATGGAGAC CCCACACAGA TAGAGGTGCG TCCCCGCCAG
    TGGGTGCACA ACGCCTTCCA CTTCGACAAC GTGCTTTCGG CCATGATGTC CCTCTTCACC
    GTGTCCACCT TCGAGGGATG GCCTGAGCTG CTGTACAAGG CCATTGACTC TCATGAGGAG
    GACAAGGGCC CCGTGTACAA CCACCGCGTG GAGATGGCCA TTTTCTTCAT CATCTACATC
    ATCCTTATCG CCTTCTTCAT GATGAACATC TTCGTGGGCT TTGTCATCGT CACCTTCCAG
    GAGCAGGGGG AGACTGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGCG CCAGTGCGTG
    CAGTACGCCC TGAAGGCCCG GCCCCTGAGG TGCTACATCC CCAAGAACCC CTACCAGTAC
    CGGGTGTGGT ACATCGTCAC CTCCTCCTAC TTCGAGTACC TGATGTTCGC CCTCATCATG
    CTCAACACCA TCTGCCTGGG CATGCAGCAC TACAACCAGT CGGAGCAAAT GAACCACATC
    TCTGACATCC TCAACGTAGC CTTCACCATC ATCTTCACAC TGGAGATGGT CCTCAAGCTC
    ATGGCGTTCA AGGCCAGGGG CTATTTTGGG GACCCCTGGA ACGTGTTTGA CTTTCTGATT
    GTCATCGGCA GCATCATCGA CGTCATCCTC AGTGAGATCG ACACTTTCCT GGCCTCCAGC
    GGGGGACTGT ATTGCCTGGG TGGCGGCTGC GGGAACGTTG ACCCGGACGA GAGCGCCCGC
    ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT GTCATGAGGC TGATCAAGCT GCTGAGCCGC
    GCGGAGGGCG TGCGCACGCT GCTCTGGACT TTCCATCCTC GCCCGCAGGC CCTGCCCTAC
    GTGGCTCTGC TCATCGTCAT GCTCTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC
    GGGAAGATCG CCTTGGTGGA CGGGACCCAG ATCAACCGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC GCCACGGGGG AGGCGTGGCA GGAGATCCTG
    CTGGCCTGCA GCTACGGCCA GCTGTGTGAC CCCGAGTCGG ACTACGCCCC CGGGGAGGAG
    TACACCTGTG GCACGGACTT CGCCTACTAC TACTTCCTCA GCTTCTACAT GCTGTGCGCC
    TTCCTGATCA TCAACCTCTT TGTGGCCGTC ATCATGGACA ACTTTGACTA CCTGACACGG
    GACTGGTCCA TCCTGGGCCC TCATCACCTG GATGAGTTCA AGGCCATCTG GGCGGAGTAC
    GACCCGGAGG CTACGGGCAG GATCAAGCAC CTGGACGTGG TGACCCTGCT GAGGAGGATC
    CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC CCCCACCGGG TAGCGTGTAA GCGGCTGGTG
    GGCATGAACA TGCCCCTCAA CAGTGACGGC ACGGTCACCT TCAACGCCAC GCTCTTCGCC
    CTGGTGCGCA CAGCTCTGAA GATCAAGACG GAAGGTAACT TCGAGCAGGC CAACGAGGAG
    CTGCGAGCCA TCATCAAGAA GGTCTGGAAG AGGACCAGCA TGAAGCTCCT GGACCAGGTC
    ATCCCTCCCA TAGGAGATGA CGAGGTGACG GTGGGGAAGT TCTACGCCAC GTTCCTCATC
    CAGGAGCACT TCCGGAAGTT CATGAGGCGC CAGGAGGAGT ATTATGGGTA CCGGCCCAAG
    AAGGACACCG TGCAGATCCA GGCTGGACTG AGGACCATCG AGGAAGAGGC AGCCCCTGAG
    ATCCGCCGCA CCATCTCGGG AGACCTGATG GCCGAAGAGG AGCTGGAGAG AGCCATGGTG
    GAGGCCGCGA TGGAGGAAGG GATCTTCCGG AGGACCGGCG GCCTGTTTGG CCAGGTGGAC
    AGCTTCCTGG AAAGGACCAA CTCGTTGGCC CCGCACATGG CCAACCAGAG ACCCCTCCAG
    TTTACTGAAA TTGAGATGGA GGAGATGGAA TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA
    CGTCCGAGTG CCCACCCCCT TGCTCGTGCC AACACCAACA ACGCCAACGC CAACGTCGCC
    AGTGGCAACG GCAACCATGG CAACAGCCCA GCATTTCCCA GTGTCCACTA CGAAGGGGAG
    CTCGCAGGGG AGACAGAGAC GCCTGCCACC AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG
    GCCCTGGGGC CCCACAGCAA GTCCCGAGTA GAGTGGCCGC AGGGACAGCT GCCCCGGAGA
    AGGACGCCCA GGGCCCAGGC CCCCGCTGCC TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG
    GTGCCCGAGG AGAGGAGCCC CACCCCAGGG TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT
    AGGACAGAGG ACGGAACCCC TGGGGGTCCA GCTCCAGCCA TGGCCCTGCT GATCCGCGAG
    GCTCTGGTTC AAGGGGGCCT GGACAGCTTG GCGGCGGATG CTAACTTCAT CATGGCCACG
    GGCCAGGCGC TGGCAGAGGC CTGCCGGATG GAACCGCGGG AAGTGGAGGT GGCAGCCGCA
    GAGCTTCTGC AGGGACGCGA GGCCCTGGAG GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC
    CTCGGGTCCT CCCTGGGCAG CCTCGACCTG CGTGAGGGCT CCCAGGAGAC CCTGATTCCC
    CGCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93088
    Clone ID Related Accession (Same CDS sequence) XM_015475322.1 , XM_015466951.1
    Accession Version XM_015466951.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5532bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1S
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
    CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG
    CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC
    ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC
    AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG
    GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC
    GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG
    CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC
    GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC
    AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC
    CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG
    AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG
    TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC
    TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG
    GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG
    CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC
    TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA
    ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC
    TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG
    ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG
    ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG
    ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC
    TGCTTCGTGG TGTGCAGTGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG
    CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GCATCTTCAA GATCACCAAG
    TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC
    TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC
    TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC
    CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG
    TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC
    TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT
    GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG
    CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG
    GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG
    AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC
    TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG
    CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC
    ATCTTCAGCC CCACCAACAA GTGGGTGGCT CCTCGGCGGT GGGGCAGGGT GATGGTGGGG
    TCTCAGAGAA GGCTGCTTCC TGCTCCGAGA AGACCGCCTT CTCCCAACTC TCTGGGCCTA
    CAGTTACACT GTGAGAAAGG CATTCCTTTA ACTTCAGATC TCTTGCTCCA TTTAACGTGC
    CAGCATCCCC AGCAGACGTA TATTTTAAGC CTCACGCGTT ACTTATACCC AAGCGTGACC
    AGATACCTCC TGCTCTGCCA CCAGATCCTC GGGTATTTTG ACATCGGGTT CACCTCTGTC
    TTCACTGTGG AGATTGTCCT CAAGATGACC ACCTACGGGG CCTTCCTGCA CAAGGGCTCC
    TTTTGCCCAA ATGAAACATT CCGTGGGCAC CCAGTGTCTG TCTCCCTCCT TCCCTCCGGG
    ATACAGGGGA AGTTCTTCAG CTGCAATGAC TTGTCCAAGA TGACGGAGGA GGAGTGCAGG
    GGCTACTACT ATGTGTACAA AGATGGAGAC CCCACACAGA TAGAGGTGCG TCCCCGCCAG
    TGGGTGCACA ACGCCTTCCA CTTCGACAAC GTGCTTTCGG CCATGATGTC CCTCTTCACC
    GTGTCCACCT TCGAGGGATG GCCTGAGCTG CTGTACAAGG CCATTGACTC TCATGAGGAG
    GACAAGGGCC CCGTGTACAA CCACCGCGTG GAGATGGCCA TTTTCTTCAT CATCTACATC
    ATCCTTATCG CCTTCTTCAT GATGAACATC TTCGTGGGCT TTGTCATCGT CACCTTCCAG
    GAGCAGGGGG AGACTGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGCG CCAGTGCGTG
    CAGTACGCCC TGAAGGCCCG GCCCCTGAGG TGCTACATCC CCAAGAACCC CTACCAGTAC
    CGGGTGTGGT ACATCGTCAC CTCCTCCTAC TTCGAGTACC TGATGTTCGC CCTCATCATG
    CTCAACACCA TCTGCCTGGG CATGCAGCAC TACAACCAGT CGGAGCAAAT GAACCACATC
    TCTGACATCC TCAACGTAGC CTTCACCATC ATCTTCACAC TGGAGATGGT CCTCAAGCTC
    ATGGCGTTCA AGGCCAGGGG CTATTTTGGG GACCCCTGGA ACGTGTTTGA CTTTCTGATT
    GTCATCGGCA GCATCATCGA CGTCATCCTC AGTGAGATCG ACACTTTCCT GGCCTCCAGC
    GGGGGACTGT ATTGCCTGGG TGGCGGCTGC GGGAACGTTG ACCCGGACGA GAGCGCCCGC
    ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT GTCATGAGGC TGATCAAGCT GCTGAGCCGC
    GCGGAGGGCG TGCGCACGCT GCTCTGGACT TTCCATCCTC GCCCGCAGGC CCTGCCCTAC
    GTGGCTCTGC TCATCGTCAT GCTCTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC
    GGGAAGATCG CCTTGGTGGA CGGGACCCAG ATCAACCGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC GCCACGGGGG AGGCGTGGCA GGAGATCCTG
    CTGGCCTGCA GCTACGGCCA GCTGTGTGAC CCCGAGTCGG ACTACGCCCC CGGGGAGGAG
    TACACCTGTG GCACGGACTT CGCCTACTAC TACTTCCTCA GCTTCTACAT GCTGTGCGCC
    TTCCTGATCA TCAACCTCTT TGTGGCCGTC ATCATGGACA ACTTTGACTA CCTGACACGG
    GACTGGTCCA TCCTGGGCCC TCATCACCTG GATGAGTTCA AGGCCATCTG GGCGGAGTAC
    GACCCGGAGG CTACGGGCAG GATCAAGCAC CTGGACGTGG TGACCCTGCT GAGGAGGATC
    CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC CCCCACCGGG TAGCGTGTAA GCGGCTGGTG
    GGCATGAACA TGCCCCTCAA CAGTGACGGC ACGGTCACCT TCAACGCCAC GCTCTTCGCC
    CTGGTGCGCA CAGCTCTGAA GATCAAGACG GAAGGTAACT TCGAGCAGGC CAACGAGGAG
    CTGCGAGCCA TCATCAAGAA GGTCTGGAAG AGGACCAGCA TGAAGCTCCT GGACCAGGTC
    ATCCCTCCCA TAGGAGATGA CGAGGTGACG GTGGGGAAGT TCTACGCCAC GTTCCTCATC
    CAGGAGCACT TCCGGAAGTT CATGAGGCGC CAGGAGGAGT ATTATGGGTA CCGGCCCAAG
    AAGGACACCG TGCAGATCCA GGCTGGACTG AGGACCATCG AGGAAGAGGC AGCCCCTGAG
    ATCCGCCGCA CCATCTCGGG AGACCTGATG GCCGAAGAGG AGCTGGAGAG AGCCATGGTG
    GAGGCCGCGA TGGAGGAAGG GATCTTCCGG AGGACCGGCG GCCTGTTTGG CCAGGTGGAC
    AGCTTCCTGG AAAGGACCAA CTCGTTGGCC CCGCACATGG CCAACCAGAG ACCCCTCCAG
    TTTACTGAAA TTGAGATGGA GGAGATGGAA TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA
    CGTCCGAGTG CCCACCCCCT TGCTCGTGCC AACACCAACA ACGCCAACGC CAACGTCGCC
    AGTGGCAACG GCAACCATGG CAACAGCCCA GCATTTCCCA GTGTCCACTA CGAAGGGGAG
    CTCGCAGGGG AGACAGAGAC GCCTGCCACC AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG
    GCCCTGGGGC CCCACAGCAA GTCCCGAGTA GAGTGGCCGC AGGGACAGCT GCCCCGGAGA
    AGGACGCCCA GGGCCCAGGC CCCCGCTGCC TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG
    GTGCCCGAGG AGAGGAGCCC CACCCCAGGG TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT
    AGGACAGAGG ACGGAACCCC TGGGGGTCCA GCTCCAGCCA TGGCCCTGCT GATCCGCGAG
    GCTCTGGTTC AAGGGGGCCT GGACAGCTTG GCGGCGGATG CTAACTTCAT CATGGCCACG
    GGCCAGGCGC TGGCAGAGGC CTGCCGGATG GAACCGCGGG AAGTGGAGGT GGCAGCCGCA
    GAGCTTCTGC AGGGACGCGA GGCCCTGGAG GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC
    CTCGGGTCCT CCCTGGGCAG CCTCGACCTG CGTGAGGGCT CCCAGGAGAC CCTGATTCCC
    CGCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322437.1
    CDS95..5626
    Translation

    Target ORF information:

    RefSeq Version XM_015466951.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1S subunit (CACNA1S), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466951.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGAGCCGG CCCCGGCACA GGACGAGGGC CTGCGGAGGA AGCAGGCCAA GAAGCCCGTC 
    CCGGAGGTCC TGCCCAGGCC ACCCCGGGCC TTGTTCTGCT TGACCCTCCA GAACCCCGTG
    CGGAAAGCCT GCATCAGCAT CGTCGAGTGG AAACCCTTCG AGACCATCAT CTTACTCACC
    ATCTTTGCCA ACTGCGTGGC CCTGGCCGTG TACCTGCCCA TGCCGGAGGA CGACAACAAC
    AGACTGAACC TCGGCCTGGA GAAGCTGGAA TACTTCTTCC TCATCGTCTT CTCCATCGAG
    GCCGCCATGA AGATCATCGC CTACGGCTTC CTGTTCCACC AGGATGCCTA CCTGCGCAGC
    GGCTGGAACG TGCTGGATTT CATCATCGTC TTCCTGGGGG TCTTCACCGT GATCCTGGAG
    CAGGTCAACC TCATCCAGAG CAACACGGCC CCGATGAGCA GCAAAGGCGC GGGCCTGGAC
    GTCAAGGCAC TGCGGGCCTT CCGCGTGCTC CGTCCGCTCC GGCTGGTGTC CGGGGTGCCC
    AGCCTGCAGG TGGTCCTCAA CTCCATCTTC AAGGCCATGC TGCCGCTCTT CCACATCGCC
    CTGCTGGTGC TCTTCATGGT CGTCATCTAC GCCATCATCG GCCTCGAGCT CTTCAAGGGC
    AAGATGCACA AGACCTGCTA CTTCATCGGC ACAGACATCG TGGCCACGGT GGAGAACGAG
    AAGCCGTCCC CCTGCGCCCG GACGGGCTCC GGCCGGCCCT GCACCATCAG CGGCAGCGAG
    TGCCGTGGCG GGTGGCCCGG CCCCAACCAC GGCATCACGC ACTTCGACAA CTTCGGCTTC
    TCCATGCTCA CCGTCTACCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTGAATGATG CTATCGGGAA CGAGTGGCCC TGGATCTACT TTGTCACCCT CATCCTGCTG
    GGCTCCTTCT TCATCCTCAA CCTGGTGCTG GGCGTCCTGA GCGGGGAATT CACCAAGGAG
    CGGGAGAAGG CCAAGTCCAG GGGGACCTTC CAGAAGCTGC GGGAGAAGCA GCAGCTGGAG
    GAGGACCTCC GGGGCTACCT GAGCTGGATC ACGCAGGGCG AGGTCATGGA CGTGGATGAC
    TTGAGAGAAG GGAAGCTGGC TCTGGATGAA GGCGGCTCCG ACACGGAGAG CCTGTACGAA
    ATCGCGGGCC TGAACAAAGT TATCCAGTTC ATCCGACACT GGAGGCAGTG GAACCGTGTC
    TTCCGCTGGA AGTGCCACGA TGTGGTGAAG TCCAGGGTGT TCTACTGGCT GGTCATCCTG
    ATCGTCGCTC TCAACACACT GTCGATCGCC TCAGAGCACC ACCACCAGCC TCTCTGGTTG
    ACCCACCTGC AAGACGTGGC CAACCGGGTG CTGCTGGCCC TGTTCACCGT GGAGATGCTG
    ATGAAGATGT ACGGGCTGGG CCTGCGCCAG TACTTCATGT CCATCTTCAA CCGCTTCGAC
    TGCTTCGTGG TGTGCAGTGG CCTCCTGGAG ATCCTGCTGG TGGAGTCGGG TGCCATGTCG
    CCCCTGGGCA TCTCGGTGCT GCGCTGCATC CGTCTGCTGC GCATCTTCAA GATCACCAAG
    TACTGGACGT CGCTGAGCAA CCTGGTGGCG TCGCTGCTCA ACTCCATCCG CTCCATCGCC
    TCGCTGCTGC TGCTGCTCTT CCTGTTCATC GTCATCTTCG CCCTGCTGGG CATGCAGCTC
    TTCGGGGGCA GGTACGACTT CGAGGACACC GAGGTGCGGC GTAGCAACTT CGACAGCTTC
    CCACAGGCCC TCATCAGCGT CTTCCAGGTG CTGACAGGAG AGGACTGGAA CTCCGTGATG
    TACAACGGGA TCATGGCCTA CGGCGGCCCA TCCTACCCCG GGGTGCTCGT CTGCATTTAC
    TTCATCATCC TGTTTGTTTG CGGCAACTAC ATCCTGCTCA ACGTCTTCCT GGCCATCGCT
    GTGGACAACC TGGCAGAGGC CGAGAGCCTG ACTTCTGCCC AGAAGGCCAA GGCTGAAGAG
    CGGAGACGCA GGAAGATGTC CAAGGGTCTC CCGGACAAGT CAGAGGAGGA GAAGGTGACG
    GTGGCCAAGA AGCTGGAGCA GAAACCCAAG GGGGAAGGCA TCCCCACCAC CGCCAAGCTG
    AAGATCGACG AGTTTGAATC CAACGTCAAC GAGGTGAAGG ACCCCTACCC CTCAGCCGAC
    TTCCCAGGGG ACGACGAGGA AGACGAGCCC GAGGTCCCAA TAAGCCCCCG GCCCCGTCCG
    CTGGCTGAGC TGCAGCTGAA GGAGAAGGCG GTGCCCATTC CAGAAGCCAG TGCCTTCTTC
    ATCTTCAGCC CCACCAACAA GTGGGTGGCT CCTCGGCGGT GGGGCAGGGT GATGGTGGGG
    TCTCAGAGAA GGCTGCTTCC TGCTCCGAGA AGACCGCCTT CTCCCAACTC TCTGGGCCTA
    CAGTTACACT GTGAGAAAGG CATTCCTTTA ACTTCAGATC TCTTGCTCCA TTTAACGTGC
    CAGCATCCCC AGCAGACGTA TATTTTAAGC CTCACGCGTT ACTTATACCC AAGCGTGACC
    AGATACCTCC TGCTCTGCCA CCAGATCCTC GGGTATTTTG ACATCGGGTT CACCTCTGTC
    TTCACTGTGG AGATTGTCCT CAAGATGACC ACCTACGGGG CCTTCCTGCA CAAGGGCTCC
    TTTTGCCCAA ATGAAACATT CCGTGGGCAC CCAGTGTCTG TCTCCCTCCT TCCCTCCGGG
    ATACAGGGGA AGTTCTTCAG CTGCAATGAC TTGTCCAAGA TGACGGAGGA GGAGTGCAGG
    GGCTACTACT ATGTGTACAA AGATGGAGAC CCCACACAGA TAGAGGTGCG TCCCCGCCAG
    TGGGTGCACA ACGCCTTCCA CTTCGACAAC GTGCTTTCGG CCATGATGTC CCTCTTCACC
    GTGTCCACCT TCGAGGGATG GCCTGAGCTG CTGTACAAGG CCATTGACTC TCATGAGGAG
    GACAAGGGCC CCGTGTACAA CCACCGCGTG GAGATGGCCA TTTTCTTCAT CATCTACATC
    ATCCTTATCG CCTTCTTCAT GATGAACATC TTCGTGGGCT TTGTCATCGT CACCTTCCAG
    GAGCAGGGGG AGACTGAGTA CAAGAACTGT GAGCTGGACA AGAACCAGCG CCAGTGCGTG
    CAGTACGCCC TGAAGGCCCG GCCCCTGAGG TGCTACATCC CCAAGAACCC CTACCAGTAC
    CGGGTGTGGT ACATCGTCAC CTCCTCCTAC TTCGAGTACC TGATGTTCGC CCTCATCATG
    CTCAACACCA TCTGCCTGGG CATGCAGCAC TACAACCAGT CGGAGCAAAT GAACCACATC
    TCTGACATCC TCAACGTAGC CTTCACCATC ATCTTCACAC TGGAGATGGT CCTCAAGCTC
    ATGGCGTTCA AGGCCAGGGG CTATTTTGGG GACCCCTGGA ACGTGTTTGA CTTTCTGATT
    GTCATCGGCA GCATCATCGA CGTCATCCTC AGTGAGATCG ACACTTTCCT GGCCTCCAGC
    GGGGGACTGT ATTGCCTGGG TGGCGGCTGC GGGAACGTTG ACCCGGACGA GAGCGCCCGC
    ATCTCCAGCG CCTTCTTCCG CCTGTTCCGT GTCATGAGGC TGATCAAGCT GCTGAGCCGC
    GCGGAGGGCG TGCGCACGCT GCTCTGGACT TTCCATCCTC GCCCGCAGGC CCTGCCCTAC
    GTGGCTCTGC TCATCGTCAT GCTCTTCTTC ATCTACGCGG TCATCGGCAT GCAGATGTTC
    GGGAAGATCG CCTTGGTGGA CGGGACCCAG ATCAACCGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCGG TGCTGCTGCT CTTCAGGTGC GCCACGGGGG AGGCGTGGCA GGAGATCCTG
    CTGGCCTGCA GCTACGGCCA GCTGTGTGAC CCCGAGTCGG ACTACGCCCC CGGGGAGGAG
    TACACCTGTG GCACGGACTT CGCCTACTAC TACTTCCTCA GCTTCTACAT GCTGTGCGCC
    TTCCTGATCA TCAACCTCTT TGTGGCCGTC ATCATGGACA ACTTTGACTA CCTGACACGG
    GACTGGTCCA TCCTGGGCCC TCATCACCTG GATGAGTTCA AGGCCATCTG GGCGGAGTAC
    GACCCGGAGG CTACGGGCAG GATCAAGCAC CTGGACGTGG TGACCCTGCT GAGGAGGATC
    CAGCCCCCGC TGGGCTTCGG GAAGTTCTGC CCCCACCGGG TAGCGTGTAA GCGGCTGGTG
    GGCATGAACA TGCCCCTCAA CAGTGACGGC ACGGTCACCT TCAACGCCAC GCTCTTCGCC
    CTGGTGCGCA CAGCTCTGAA GATCAAGACG GAAGGTAACT TCGAGCAGGC CAACGAGGAG
    CTGCGAGCCA TCATCAAGAA GGTCTGGAAG AGGACCAGCA TGAAGCTCCT GGACCAGGTC
    ATCCCTCCCA TAGGAGATGA CGAGGTGACG GTGGGGAAGT TCTACGCCAC GTTCCTCATC
    CAGGAGCACT TCCGGAAGTT CATGAGGCGC CAGGAGGAGT ATTATGGGTA CCGGCCCAAG
    AAGGACACCG TGCAGATCCA GGCTGGACTG AGGACCATCG AGGAAGAGGC AGCCCCTGAG
    ATCCGCCGCA CCATCTCGGG AGACCTGATG GCCGAAGAGG AGCTGGAGAG AGCCATGGTG
    GAGGCCGCGA TGGAGGAAGG GATCTTCCGG AGGACCGGCG GCCTGTTTGG CCAGGTGGAC
    AGCTTCCTGG AAAGGACCAA CTCGTTGGCC CCGCACATGG CCAACCAGAG ACCCCTCCAG
    TTTACTGAAA TTGAGATGGA GGAGATGGAA TCGCCCGTCT TCCTGGAGGA CTTCCCTCAA
    CGTCCGAGTG CCCACCCCCT TGCTCGTGCC AACACCAACA ACGCCAACGC CAACGTCGCC
    AGTGGCAACG GCAACCATGG CAACAGCCCA GCATTTCCCA GTGTCCACTA CGAAGGGGAG
    CTCGCAGGGG AGACAGAGAC GCCTGCCACC AGAGGAGGAC TCTTCGGCCA GCCCCGGGGG
    GCCCTGGGGC CCCACAGCAA GTCCCGAGTA GAGTGGCCGC AGGGACAGCT GCCCCGGAGA
    AGGACGCCCA GGGCCCAGGC CCCCGCTGCC TCCTGCCAGC ACGCGGGAGC AGAGTCCCAG
    GTGCCCGAGG AGAGGAGCCC CACCCCAGGG TCCCTGCCCG GGGAGGCGCC GCCCGGCAGT
    AGGACAGAGG ACGGAACCCC TGGGGGTCCA GCTCCAGCCA TGGCCCTGCT GATCCGCGAG
    GCTCTGGTTC AAGGGGGCCT GGACAGCTTG GCGGCGGATG CTAACTTCAT CATGGCCACG
    GGCCAGGCGC TGGCAGAGGC CTGCCGGATG GAACCGCGGG AAGTGGAGGT GGCAGCCGCA
    GAGCTTCTGC AGGGACGCGA GGCCCTGGAG GGCGTGCCTG GTGCCCGAGG GTGCCCAGGC
    CTCGGGTCCT CCCTGGGCAG CCTCGACCTG CGTGAGGGCT CCCAGGAGAC CCTGATTCCC
    CGCAGGCCAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.