KIF1B gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015475109.1	XP_015330595.1	kinesin-like protein KIF1B isoform X3
XM_010813295.2	XP_010811597.1	kinesin-like protein KIF1B isoform X12
XM_010823093.2	XP_010821395.1	kinesin-like protein KIF1B isoform X12
XM_010823094.2	XP_010821396.2	kinesin-like protein KIF1B isoform X14
XM_005216995.4	XP_005217052.2	kinesin-like protein KIF1B isoform X5
XM_024976731.1	XP_024832499.1	kinesin-like protein KIF1B isoform X6
XM_024976732.1	XP_024832500.1	kinesin-like protein KIF1B isoform X8
XM_005216992.3	XP_005217049.1	kinesin-like protein KIF1B isoform X6
XM_010823091.2	XP_010821393.2	kinesin-like protein KIF1B isoform X8
XM_003583233.4	XP_003583281.3	kinesin-like protein KIF1B isoform X10
XM_015466788.1	XP_015322274.1	kinesin-like protein KIF1B isoform X11
XM_024976730.1	XP_024832498.1	kinesin-like protein KIF1B isoform X2
XM_010813295.3	XP_010811597.1	kinesin-like protein KIF1B isoform X9
XM_005216993.3	XP_005217050.1	kinesin-like protein KIF1B isoform X8
XM_005216995.3	XP_005217052.1	kinesin-like protein KIF1B isoform X10
XM_015475113.1	XP_015330599.1	kinesin-like protein KIF1B isoform X11
XM_015466782.1	XP_015322268.1	kinesin-like protein KIF1B isoform X1
XM_015466783.1	XP_015322269.1	kinesin-like protein KIF1B isoform X2
XM_024976729.1	XP_024832497.1	kinesin-like protein KIF1B isoform X1
XM_005216993.4	XP_005217050.2	kinesin-like protein KIF1B isoform X3
XM_015466786.1	XP_015322272.1	kinesin-like protein KIF1B isoform X5
XM_015475107.1	XP_015330593.1	kinesin-like protein KIF1B isoform X1
XM_015475110.1	XP_015330596.1	kinesin-like protein KIF1B isoform X4
XM_015475112.1	XP_015330598.1	kinesin-like protein KIF1B isoform X7
XM_002694045.5	XP_002694091.3	kinesin-like protein KIF1B isoform X9
XM_005216997.3	XP_005217054.1	kinesin-like protein KIF1B isoform X14
XM_015466787.1	XP_015322273.1	kinesin-like protein KIF1B isoform X7
XM_010823092.2	XP_010821394.2	kinesin-like protein KIF1B isoform X9
XM_005194809.3	XP_005194866.1	kinesin-like protein KIF1B isoform X13
XM_002694045.6	XP_002694091.4	kinesin-like protein KIF1B isoform X4
XM_005216998.4	XP_005217055.1	kinesin-like protein KIF1B isoform X11
XM_015475108.1	XP_015330594.1	kinesin-like protein KIF1B isoform X2
XM_015475111.1	XP_015330597.1	kinesin-like protein KIF1B isoform X5
XM_005216998.3	XP_005217055.1	kinesin-like protein KIF1B isoform X13
XM_015466784.1	XP_015322270.1	kinesin-like protein KIF1B isoform X3
XM_015466785.1	XP_015322271.1	kinesin-like protein KIF1B isoform X4
XM_010823090.2	XP_010821392.2	kinesin-like protein KIF1B isoform X6
XM_005216992.4	XP_005217049.2	kinesin-like protein KIF1B isoform X1
XM_005216997.4	XP_005217054.2	kinesin-like protein KIF1B isoform X7
XM_024976733.1	XP_024832501.1	kinesin-like protein KIF1B isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa223927	XM_010813295.3 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223928	XM_024976733.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa223929	XM_005216998.4 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa27234	XM_005216998.3 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa27234	XM_005194809.3 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa60159	XM_010823093.2 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa60159	XM_010813295.2 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa223919	XM_005216992.4 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223919	XM_024976729.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223920	XM_024976730.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223921	XM_005216993.4 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223922	XM_002694045.6 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223923	XM_005216995.4 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223924	XM_024976731.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223925	XM_005216997.4 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223926	XM_024976732.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa92942	XM_015475107.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92942	XM_015466782.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92943	XM_015475108.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92943	XM_015466783.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92944	XM_015466784.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92944	XM_015475109.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92945	XM_015466785.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92945	XM_015475110.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92946	XM_015466786.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92946	XM_015475111.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92947	XM_015475112.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92947	XM_015466787.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92948	XM_015466788.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa92948	XM_015475113.1 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa27227	XM_010823090.2 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27227	XM_005216992.3 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27228	XM_005216993.3 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27228	XM_010823091.2 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27230	XM_010823092.2 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27230	XM_002694045.5 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27231	XM_003583233.4 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27231	XM_005216995.3 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27233	XM_010823094.2 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa27233	XM_005216997.3 Latest version!	Bos taurus kinesin family member 1B (KIF1B), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

KIF1B ( XM_010813295.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811597.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa223927
Clone ID Related Accession (Same CDS sequence)	XM_010813295.3
Accession Version	XM_010813295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813295.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACGC GGATTCTGAT AGCGGGGATG ATTCTGACAA GAGATCCTGC 
GAAGAGAGCT GGAAACTGAT TACTTCTCTG AGAGAAAAGC TGCCTCCCAG CAAGCTGCAA 
ACCATTGTTA AAAAATGCGG CCTCCCAAGC AGTGGGAAGA AACGGGAACC AATAAAAATG 
TATCAGATCC CCCAGAGAAG ACGCCTGAGT AAAGACTCCA AGTGGGTCAC CATCTCAGAT 
CTCAAGATTC AGGCTGTGAA AGAGATATGC TACGAGGTTG CTCTCAATGA TTTTCGGCAT 
AGTCGGCAGG AGATTGAAGC CTTGGCCATT GTTAAGATGA AAGAACTTTG TGCCATGTAT 
GGGAAGAAAG ATCCTAATGA GCGGGACTCC TGGAGGGCAG TGGCCAGGGA TGTCTGGGAC 
ACCGTTGGTG TAGGGGATGA GAAGATTGAG GACGTAATGG CCAGTAGTAA AGGTGGGACT 
GATGTGGATG ACCTCAAGGT TCATATAGAC AAGCTGGAAG ATATTTTGCA AGAAGTCAAA 
AAGCAAAATA ACATGAAAGA TGAGGAGATA AAAGTCTTAA GAAATAAAAT GCTCAAAATG 
GAGAAAGTCT TGCCACTGAT TGGGTCTCCA GAACAGAAAA CCCAAGGAAA CCAAAAATCA 
AAGGAACCCA GTGGTGCTGG TGCCAGTAGT AAGCCTGAGA ATGATGTAAG TAAAGGCGAC 
AGCGGAGACC TTGGTAAAGA AGAACGTGTC TCCCAGCTGA TGAACGGAGA CCCAGCTTTT 
AGACGTGGAC GTCTGCGTTG GATGAGGCAA GAGCAAATTC GGTTCAAGAA CCTGCAGCAG 
CAGGAAATAA CAAAGCAGCT TCGTCGGCAG AATGTACCTC ATCGATTCAT CCCTCCGGAG 
AACCGGAAGC CGCGGTTTCC GTTTAAGAGC AACCCTAAAC ACAGAAACTC ATGGAGTCCT 
GGGACACACA TCATCATCAC AGAAGATGAG GTAATAGAGC TTAGGATTCC CAAAGAGGAA 
GATGGAAGGA AAGGAAATAA GCCGGAGAAC CAAGAAAAGG GTACTAGAGC AGCTTCTAAG 
GATCCCCAGT TACCATGGGG CCCTCAGGGC ACTCGAAGTC AAGATCACAT TCAGGTTAGC 
AAGCAGCACA TTAATAATCA ACAACAGCCA CCTCAGTTAC GCTGGAGAAG CAATTCTCTC 
AATAACGGCC AACCGAAAAG TACGCGCTGC CAGGCATCTG CTTCCCCAGA AGTGCTCGCC 
TCCCACAGTG GCCACCCCAC CGCTGACCTG CAGACTTTTC AGGCGAAACG CCACATGCAC 
CAGCACCGTC AAGCTTACTG TAGTTATAAC ACTGGAGCTC AGCTGGAGGG CAGTGCAGCC 
ACTTCTTGTC AGAAGCAGAC TGACAGACCC AACCACTGTA GCCAGTTTGT GACACCTCCG 
AGAATGAGGC GACAGTTCTC GGCACCCAAT CTCAAAACTG GTCGGGAAAC CACAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811597.1
CDS	380..3979
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKCGLPSSGKKREPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKMKELCAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMASSKGGTDVDDLKVHIDKLEDILQEVKKQNNMKDEEIKVLRNKMLKMEKVLPLIGSPEQKTQGNQKSKEPSGAGASSKPENDVSKGDSGDLGKEERVSQLMNGDPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKPRFPFKSNPKHRNSWSPGTHIIITEDEVIELRIPKEEDGRKGNKPENQEKGTRAASKDPQLPWGPQGTRSQDHIQVSKQHINNQQQPPQLRWRSNSLNNGQPKSTRCQASASPEVLASHSGHPTADLQTFQAKRHMHQHRQAYCSYNTGAQLEGSAATSCQKQTDRPNHCSQFVTPPRMRRQFSAPNLKTGRETTV

Target ORF information:

RefSeq Version	XM_010813295.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813295.3

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACGC GGATTCTGAT AGCGGGGATG ATTCTGACAA GAGATCCTGC 
GAAGAGAGCT GGAAACTGAT TACTTCTCTG AGAGAAAAGC TGCCTCCCAG CAAGCTGCAA 
ACCATTGTTA AAAAATGCGG CCTCCCAAGC AGTGGGAAGA AACGGGAACC AATAAAAATG 
TATCAGATCC CCCAGAGAAG ACGCCTGAGT AAAGACTCCA AGTGGGTCAC CATCTCAGAT 
CTCAAGATTC AGGCTGTGAA AGAGATATGC TACGAGGTTG CTCTCAATGA TTTTCGGCAT 
AGTCGGCAGG AGATTGAAGC CTTGGCCATT GTTAAGATGA AAGAACTTTG TGCCATGTAT 
GGGAAGAAAG ATCCTAATGA GCGGGACTCC TGGAGGGCAG TGGCCAGGGA TGTCTGGGAC 
ACCGTTGGTG TAGGGGATGA GAAGATTGAG GACGTAATGG CCAGTAGTAA AGGTGGGACT 
GATGTGGATG ACCTCAAGGT TCATATAGAC AAGCTGGAAG ATATTTTGCA AGAAGTCAAA 
AAGCAAAATA ACATGAAAGA TGAGGAGATA AAAGTCTTAA GAAATAAAAT GCTCAAAATG 
GAGAAAGTCT TGCCACTGAT TGGGTCTCCA GAACAGAAAA CCCAAGGAAA CCAAAAATCA 
AAGGAACCCA GTGGTGCTGG TGCCAGTAGT AAGCCTGAGA ATGATGTAAG TAAAGGCGAC 
AGCGGAGACC TTGGTAAAGA AGAACGTGTC TCCCAGCTGA TGAACGGAGA CCCAGCTTTT 
AGACGTGGAC GTCTGCGTTG GATGAGGCAA GAGCAAATTC GGTTCAAGAA CCTGCAGCAG 
CAGGAAATAA CAAAGCAGCT TCGTCGGCAG AATGTACCTC ATCGATTCAT CCCTCCGGAG 
AACCGGAAGC CGCGGTTTCC GTTTAAGAGC AACCCTAAAC ACAGAAACTC ATGGAGTCCT 
GGGACACACA TCATCATCAC AGAAGATGAG GTAATAGAGC TTAGGATTCC CAAAGAGGAA 
GATGGAAGGA AAGGAAATAA GCCGGAGAAC CAAGAAAAGG GTACTAGAGC AGCTTCTAAG 
GATCCCCAGT TACCATGGGG CCCTCAGGGC ACTCGAAGTC AAGATCACAT TCAGGTTAGC 
AAGCAGCACA TTAATAATCA ACAACAGCCA CCTCAGTTAC GCTGGAGAAG CAATTCTCTC 
AATAACGGCC AACCGAAAAG TACGCGCTGC CAGGCATCTG CTTCCCCAGA AGTGCTCGCC 
TCCCACAGTG GCCACCCCAC CGCTGACCTG CAGACTTTTC AGGCGAAACG CCACATGCAC 
CAGCACCGTC AAGCTTACTG TAGTTATAAC ACTGGAGCTC AGCTGGAGGG CAGTGCAGCC 
ACTTCTTGTC AGAAGCAGAC TGACAGACCC AACCACTGTA GCCAGTTTGT GACACCTCCG 
AGAATGAGGC GACAGTTCTC GGCACCCAAT CTCAAAACTG GTCGGGAAAC CACAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_024976733.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832501.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.

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CloneID	OBa223928
Clone ID Related Accession (Same CDS sequence)	XM_024976733.1
Accession Version	XM_024976733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TATTGATCCA TTGATCGATG ATTACTCGGG AAGTGGAGGC 
AAATCATCCA TGGGGTCCCT TACTTCATCT CCATCTTCCT GCTCACTCAA TAGTCAGGTG 
GGCTTAACAT CTGTGACCAG TATTCAGGAG AGAATCATGT CTACACCTGG AGGAGAGGAA 
GCCATCGAAC GTCTGAAGGA ATCAGAGAAG ATCATTGCTG AGTTGAATGA AACCTGGGAA 
GAGAAGCTGC GTAAAACAGA GGCCATCAGG ATGGAGAGAG AAGCCTTGTT GGCTGAGATG 
GGAGTTGCCA TTCGGGAAGA CGGAGGAACC CTTGGGGTTT TCTCTCCTAA AAAGACACCA 
CATCTTGTTA ACCTCAATGA GGACCCTCTA ATGTCCGAGT GCCTGCTTTA TTACATCAAA 
GATGGAATTA CAAGGGTTGG CCAAGCAGAT GCTGAGCGGC GCCAGGACAT CGTGCTGAGT 
GGGGCTCACA TTAAAGAAGA GCATTGTATC TTCCGGAGCG AGAGGAACAA CAGCGGGGAT 
GTTATTGTGA CTCTGGAGCC CTGTGAACGT TCAGAAACCT ACGTAAATGG CAAGAGGGTG 
ACCCAGCCTG TTCAGCTGCG CTCAGGAAAC CGTATCATCA TGGGTAAAAA CCACGTGTTC 
CGTTTTAACC ACCCGGAGCA AGCACGGGCT GAGCGGGAAA AGACTCCTTC TGCTGAGACC 
CCCTCTGAGC CTGTGGACTG GACGTTTGCC CAGAGAGAGC TTCTGGAAAA ACAAGGAATT 
GATATGAAAC AGGAGATGGA AAAAAGGCTA CAGGAAATGG AGATCCTATA CAAAAAGGAG 
AAGGAAGAAG CAGATCTTCT TTTGGAGCAG CAGAGACTGG ACGCGGATTC TGATAGCGGG 
GATGATTCTG ACAAGAGATC CTGCGAAGAG AGCTGGAAAC TGATTACTTC TCTGAGAGAA 
AAGCTGCCTC CCAGCAAGCT GCAAACCATT GTTAAAAAAT GCGGCCTCCC AAGCAGTGGG 
AAGAAACGGG AACCAATAAA AATGTATCAG ATCCCCCAGA GAAGACGCCT GAGTAAAGAC 
TCCAAGTGGG TCACCATCTC AGATCTCAAG ATTCAGGCTG TGAAAGAGAT ATGCTACGAG 
GTTGCTCTCA ATGATTTTCG GCATAGTCGG CAGGAGATTG AAGCCTTGGC CATTGTTAAG 
ATGAAAGAAC TTTGTGCCAT GTATGGGAAG AAAGATCCTA ATGAGCGGGA CTCCTGGAGG 
GCAGTGGCCA GGGATGTCTG GGACACCGTT GGTGTAGGGG ATGAGAAGAT TGAGGACGTA 
ATGGCCAGTA GTAAAGGTGG GACTGATGTG GATGACCTCA AGGTTCATAT AGACAAGCTG 
GAAGATATTT TGCAAGAAGT CAAAAAGCAA AATAACATGA AAGATGAGGA GATAAAAGTC 
TTAAGAAATA AAATGCTCAA AATGGAGAAA GTCTTGCCAC TGATTGGGTC TCCAGAACAG 
AAAACCCAAG GAAACCAAAA ATCAAAGGAA CCCAGTGGTG CTGGTGCCAG TAGTAAGCCT 
GAGAATGATG TAAGTAAAGG CGACAGCGGA GACCTTGGTA AAGAAGAACG TGTCTCCCAG 
CTGATGAACG GAGACCCAGC TTTTAGACGT GGACGTCTGC GTTGGATGAG GCAAGAGCAA 
ATTCGGTTCA AGAACCTGCA GCAGCAGGAA ATAACAAAGC AGCTTCGTCG GCAGAATGTA 
CCTCATCGAT TCATCCCTCC GGAGAACCGG AAGCCGCGGT TTCCGTTTAA GAGCAACCCT 
AAACACAGAA ACTCATGGAG TCCTGGGACA CACATCATCA TCACAGAAGA TGAGGTAATA 
GAGCTTAGGA TTCCCAAAGA GGAAGATGGA AGGAAAGGAA ATAAGCCGGA GAACCAAGAA 
AAGGGTACTA GAGCAGCTTC TAAGGATCCC CAGTTACCAT GGGGCCCTCA GGGCACTCGA 
AGTCAAGATC ACATTCAGGT TAGCAAGCAG CACATTAATA ATCAACAACA GCCACCTCAG 
TTACGCTGGA GAAGCAATTC TCTCAATAAC GGCCAACCGA AAAGTACGCG CTGCCAGGCA 
TCTGCTTCCC CAGAAGTGCT CGCCTCCCAC AGTGGCCACC CCACCGCTGA CCTGCAGACT 
TTTCAGGCGA AACGCCACAT GCACCAGCAC CGTCAAGCTT ACTGTAGTTA TAACACTGGA 
GCTCAGCTGG AGGGCAGTGC AGCCACTTCT TGTCAGAAGC AGACTGACAG ACCCAACCAC 
TGTAGCCAGT TTGTGACACC TCCGAGAATG AGGCGACAGT TCTCGGCACC CAATCTCAAA 
ACTGGTCGGG AAACCACAGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832501.1
CDS	380..3883
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKSSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKCGLPSSGKKREPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKMKELCAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMASSKGGTDVDDLKVHIDKLEDILQEVKKQNNMKDEEIKVLRNKMLKMEKVLPLIGSPEQKTQGNQKSKEPSGAGASSKPENDVSKGDSGDLGKEERVSQLMNGDPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKPRFPFKSNPKHRNSWSPGTHIIITEDEVIELRIPKEEDGRKGNKPENQEKGTRAASKDPQLPWGPQGTRSQDHIQVSKQHINNQQQPPQLRWRSNSLNNGQPKSTRCQASASPEVLASHSGHPTADLQTFQAKRHMHQHRQAYCSYNTGAQLEGSAATSCQKQTDRPNHCSQFVTPPRMRRQFSAPNLKTGRETTV

Target ORF information:

RefSeq Version	XM_024976733.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976733.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TATTGATCCA TTGATCGATG ATTACTCGGG AAGTGGAGGC 
AAATCATCCA TGGGGTCCCT TACTTCATCT CCATCTTCCT GCTCACTCAA TAGTCAGGTG 
GGCTTAACAT CTGTGACCAG TATTCAGGAG AGAATCATGT CTACACCTGG AGGAGAGGAA 
GCCATCGAAC GTCTGAAGGA ATCAGAGAAG ATCATTGCTG AGTTGAATGA AACCTGGGAA 
GAGAAGCTGC GTAAAACAGA GGCCATCAGG ATGGAGAGAG AAGCCTTGTT GGCTGAGATG 
GGAGTTGCCA TTCGGGAAGA CGGAGGAACC CTTGGGGTTT TCTCTCCTAA AAAGACACCA 
CATCTTGTTA ACCTCAATGA GGACCCTCTA ATGTCCGAGT GCCTGCTTTA TTACATCAAA 
GATGGAATTA CAAGGGTTGG CCAAGCAGAT GCTGAGCGGC GCCAGGACAT CGTGCTGAGT 
GGGGCTCACA TTAAAGAAGA GCATTGTATC TTCCGGAGCG AGAGGAACAA CAGCGGGGAT 
GTTATTGTGA CTCTGGAGCC CTGTGAACGT TCAGAAACCT ACGTAAATGG CAAGAGGGTG 
ACCCAGCCTG TTCAGCTGCG CTCAGGAAAC CGTATCATCA TGGGTAAAAA CCACGTGTTC 
CGTTTTAACC ACCCGGAGCA AGCACGGGCT GAGCGGGAAA AGACTCCTTC TGCTGAGACC 
CCCTCTGAGC CTGTGGACTG GACGTTTGCC CAGAGAGAGC TTCTGGAAAA ACAAGGAATT 
GATATGAAAC AGGAGATGGA AAAAAGGCTA CAGGAAATGG AGATCCTATA CAAAAAGGAG 
AAGGAAGAAG CAGATCTTCT TTTGGAGCAG CAGAGACTGG ACGCGGATTC TGATAGCGGG 
GATGATTCTG ACAAGAGATC CTGCGAAGAG AGCTGGAAAC TGATTACTTC TCTGAGAGAA 
AAGCTGCCTC CCAGCAAGCT GCAAACCATT GTTAAAAAAT GCGGCCTCCC AAGCAGTGGG 
AAGAAACGGG AACCAATAAA AATGTATCAG ATCCCCCAGA GAAGACGCCT GAGTAAAGAC 
TCCAAGTGGG TCACCATCTC AGATCTCAAG ATTCAGGCTG TGAAAGAGAT ATGCTACGAG 
GTTGCTCTCA ATGATTTTCG GCATAGTCGG CAGGAGATTG AAGCCTTGGC CATTGTTAAG 
ATGAAAGAAC TTTGTGCCAT GTATGGGAAG AAAGATCCTA ATGAGCGGGA CTCCTGGAGG 
GCAGTGGCCA GGGATGTCTG GGACACCGTT GGTGTAGGGG ATGAGAAGAT TGAGGACGTA 
ATGGCCAGTA GTAAAGGTGG GACTGATGTG GATGACCTCA AGGTTCATAT AGACAAGCTG 
GAAGATATTT TGCAAGAAGT CAAAAAGCAA AATAACATGA AAGATGAGGA GATAAAAGTC 
TTAAGAAATA AAATGCTCAA AATGGAGAAA GTCTTGCCAC TGATTGGGTC TCCAGAACAG 
AAAACCCAAG GAAACCAAAA ATCAAAGGAA CCCAGTGGTG CTGGTGCCAG TAGTAAGCCT 
GAGAATGATG TAAGTAAAGG CGACAGCGGA GACCTTGGTA AAGAAGAACG TGTCTCCCAG 
CTGATGAACG GAGACCCAGC TTTTAGACGT GGACGTCTGC GTTGGATGAG GCAAGAGCAA 
ATTCGGTTCA AGAACCTGCA GCAGCAGGAA ATAACAAAGC AGCTTCGTCG GCAGAATGTA 
CCTCATCGAT TCATCCCTCC GGAGAACCGG AAGCCGCGGT TTCCGTTTAA GAGCAACCCT 
AAACACAGAA ACTCATGGAG TCCTGGGACA CACATCATCA TCACAGAAGA TGAGGTAATA 
GAGCTTAGGA TTCCCAAAGA GGAAGATGGA AGGAAAGGAA ATAAGCCGGA GAACCAAGAA 
AAGGGTACTA GAGCAGCTTC TAAGGATCCC CAGTTACCAT GGGGCCCTCA GGGCACTCGA 
AGTCAAGATC ACATTCAGGT TAGCAAGCAG CACATTAATA ATCAACAACA GCCACCTCAG 
TTACGCTGGA GAAGCAATTC TCTCAATAAC GGCCAACCGA AAAGTACGCG CTGCCAGGCA 
TCTGCTTCCC CAGAAGTGCT CGCCTCCCAC AGTGGCCACC CCACCGCTGA CCTGCAGACT 
TTTCAGGCGA AACGCCACAT GCACCAGCAC CGTCAAGCTT ACTGTAGTTA TAACACTGGA 
GCTCAGCTGG AGGGCAGTGC AGCCACTTCT TGTCAGAAGC AGACTGACAG ACCCAACCAC 
TGTAGCCAGT TTGTGACACC TCCGAGAATG AGGCGACAGT TCTCGGCACC CAATCTCAAA 
ACTGGTCGGG AAACCACAGT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216998.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217055.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa223929
Clone ID Related Accession (Same CDS sequence)	XM_005216998.4
Accession Version	XM_005216998.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216998.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
GCGGATTCTG ATAGCGGGGA TGATTCTGAC AAGAGATCCT GCGAAGAGAG CTGGAAACTG 
ATTACTTCTC TGAGAGAAAA GCTGCCTCCC AGCAAGCTGC AAACCATTGT TAAAAAATGC 
GGCCTCCCAA GCAGTGGGAA GAAACGGGAA CCAATAAAAA TGTATCAGAT CCCCCAGAGA 
AGACGCCTGA GTAAAGACTC CAAGTGGGTC ACCATCTCAG ATCTCAAGAT TCAGGCTGTG 
AAAGAGATAT GCTACGAGGT TGCTCTCAAT GATTTTCGGC ATAGTCGGCA GGAGATTGAA 
GCCTTGGCCA TTGTTAAGAT GAAAGAACTT TGTGCCATGT ATGGGAAGAA AGATCCTAAT 
GAGCGGGACT CCTGGAGGGC AGTGGCCAGG GATGTCTGGG ACACCGTTGG TGTAGGGGAT 
GAGAAGATTG AGGACGTAAT GGCCAGTAGT AAAGGTGGGA CTGATGTGGA TGACCTCAAG 
GTTCATATAG ACAAGCTGGA AGATATTTTG CAAGAAGTCA AAAAGCAAAA TAACATGAAA 
GATGAGGAGA TAAAAGTCTT AAGAAATAAA ATGCTCAAAA TGGAGAAAGT CTTGCCACTG 
ATTGGGTCTC CAGAACAGAA AACCCAAGGA AACCAAAAAT CAAAGGAACC CAGTGGTGCT 
GGTGCCAGTA GTAAGCCTGA GAATGATGTA AGTAAAGGCG ACAGCGGAGA CCTTGGTAAA 
GAAGAACGTG TCTCCCAGCT GATGAACGGA GACCCAGCTT TTAGACGTGG ACGTCTGCGT 
TGGATGAGGC AAGAGCAAAT TCGGTTCAAG AACCTGCAGC AGCAGGAAAT AACAAAGCAG 
CTTCGTCGGC AGAATGTACC TCATCGATTC ATCCCTCCGG AGAACCGGAA GCCGCGGTTT 
CCGTTTAAGA GCAACCCTAA ACACAGAAAC TCATGGAGTC CTGGGACACA CATCATCATC 
ACAGAAGATG AGGTAATAGA GCTTAGGATT CCCAAAGAGG AAGATGGAAG GAAAGGAAAT 
AAGCCGGAGA ACCAAGAAAA GGGTACTAGA GCAGCTTCTA AGGATCCCCA GTTACCATGG 
GGCCCTCAGG GCACTCGAAG TCAAGATCAC ATTCAGGTTA GCAAGCAGCA CATTAATAAT 
CAACAACAGC CACCTCAGTT ACGCTGGAGA AGCAATTCTC TCAATAACGG CCAACCGAAA 
AGTACGCGCT GCCAGGCATC TGCTTCCCCA GAAGTGCTCG CCTCCCACAG TGGCCACCCC 
ACCGCTGACC TGCAGACTTT TCAGGCGAAA CGCCACATGC ACCAGCACCG TCAAGCTTAC 
TGTAGTTATA ACACTGGAGC TCAGCTGGAG GGCAGTGCAG CCACTTCTTG TCAGAAGCAG 
ACTGACAGAC CCAACCACTG TAGCCAGTTT GTGACACCTC CGAGAATGAG GCGACAGTTC 
TCGGCACCCA ATCTCAAAAC TGGTCGGGAA ACCACAGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217055.1
CDS	380..3841
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKCGLPSSGKKREPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKMKELCAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMASSKGGTDVDDLKVHIDKLEDILQEVKKQNNMKDEEIKVLRNKMLKMEKVLPLIGSPEQKTQGNQKSKEPSGAGASSKPENDVSKGDSGDLGKEERVSQLMNGDPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKPRFPFKSNPKHRNSWSPGTHIIITEDEVIELRIPKEEDGRKGNKPENQEKGTRAASKDPQLPWGPQGTRSQDHIQVSKQHINNQQQPPQLRWRSNSLNNGQPKSTRCQASASPEVLASHSGHPTADLQTFQAKRHMHQHRQAYCSYNTGAQLEGSAATSCQKQTDRPNHCSQFVTPPRMRRQFSAPNLKTGRETTV

Target ORF information:

RefSeq Version	XM_005216998.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216998.4

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
GCGGATTCTG ATAGCGGGGA TGATTCTGAC AAGAGATCCT GCGAAGAGAG CTGGAAACTG 
ATTACTTCTC TGAGAGAAAA GCTGCCTCCC AGCAAGCTGC AAACCATTGT TAAAAAATGC 
GGCCTCCCAA GCAGTGGGAA GAAACGGGAA CCAATAAAAA TGTATCAGAT CCCCCAGAGA 
AGACGCCTGA GTAAAGACTC CAAGTGGGTC ACCATCTCAG ATCTCAAGAT TCAGGCTGTG 
AAAGAGATAT GCTACGAGGT TGCTCTCAAT GATTTTCGGC ATAGTCGGCA GGAGATTGAA 
GCCTTGGCCA TTGTTAAGAT GAAAGAACTT TGTGCCATGT ATGGGAAGAA AGATCCTAAT 
GAGCGGGACT CCTGGAGGGC AGTGGCCAGG GATGTCTGGG ACACCGTTGG TGTAGGGGAT 
GAGAAGATTG AGGACGTAAT GGCCAGTAGT AAAGGTGGGA CTGATGTGGA TGACCTCAAG 
GTTCATATAG ACAAGCTGGA AGATATTTTG CAAGAAGTCA AAAAGCAAAA TAACATGAAA 
GATGAGGAGA TAAAAGTCTT AAGAAATAAA ATGCTCAAAA TGGAGAAAGT CTTGCCACTG 
ATTGGGTCTC CAGAACAGAA AACCCAAGGA AACCAAAAAT CAAAGGAACC CAGTGGTGCT 
GGTGCCAGTA GTAAGCCTGA GAATGATGTA AGTAAAGGCG ACAGCGGAGA CCTTGGTAAA 
GAAGAACGTG TCTCCCAGCT GATGAACGGA GACCCAGCTT TTAGACGTGG ACGTCTGCGT 
TGGATGAGGC AAGAGCAAAT TCGGTTCAAG AACCTGCAGC AGCAGGAAAT AACAAAGCAG 
CTTCGTCGGC AGAATGTACC TCATCGATTC ATCCCTCCGG AGAACCGGAA GCCGCGGTTT 
CCGTTTAAGA GCAACCCTAA ACACAGAAAC TCATGGAGTC CTGGGACACA CATCATCATC 
ACAGAAGATG AGGTAATAGA GCTTAGGATT CCCAAAGAGG AAGATGGAAG GAAAGGAAAT 
AAGCCGGAGA ACCAAGAAAA GGGTACTAGA GCAGCTTCTA AGGATCCCCA GTTACCATGG 
GGCCCTCAGG GCACTCGAAG TCAAGATCAC ATTCAGGTTA GCAAGCAGCA CATTAATAAT 
CAACAACAGC CACCTCAGTT ACGCTGGAGA AGCAATTCTC TCAATAACGG CCAACCGAAA 
AGTACGCGCT GCCAGGCATC TGCTTCCCCA GAAGTGCTCG CCTCCCACAG TGGCCACCCC 
ACCGCTGACC TGCAGACTTT TCAGGCGAAA CGCCACATGC ACCAGCACCG TCAAGCTTAC 
TGTAGTTATA ACACTGGAGC TCAGCTGGAG GGCAGTGCAG CCACTTCTTG TCAGAAGCAG 
ACTGACAGAC CCAACCACTG TAGCCAGTTT GTGACACCTC CGAGAATGAG GCGACAGTTC 
TCGGCACCCA ATCTCAAAAC TGGTCGGGAA ACCACAGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005194809.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005194866.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa27234
Clone ID Related Accession (Same CDS sequence)	XM_005194809.3 , XM_005216998.3
Accession Version	XM_005194809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
GCGGATTCTG ATAGCGGGGA TGATTCTGAC AAGAGATCCT GCGAAGAGAG CTGGAAACTG 
ATTACTTCTC TGAGAGAAAA GCTGCCTCCC AGCAAGCTGC AAACCATTGT TAAAAAATGC 
GGCCTCCCAA GCAGTGGGAA GAAACGGGAA CCAATAAAAA TGTATCAGAT CCCCCAGAGA 
AGACGCCTGA GTAAAGACTC CAAGTGGGTC ACCATCTCAG ATCTCAAGAT TCAGGCTGTG 
AAAGAGATAT GCTACGAGGT TGCTCTCAAT GATTTTCGGC ATAGTCGGCA GGAGATTGAA 
GCCTTGGCCA TTGTTAAGAT GAAAGAACTT TGTGCCATGT ATGGGAAGAA AGATCCTAAT 
GAGCGGGACT CCTGGAGGGC AGTGGCCAGG GATGTCTGGG ACACCGTTGG TGTAGGGGAT 
GAGAAGATTG AGGACGTAAT GGCCAGTAGT AAAGGTGGGA CTGATGTGGA TGACCTCAAG 
GTTCATATAG ACAAGCTGGA AGATATTTTG CAAGAAGTCA AAAAGCAAAA TAACATGAAA 
GATGAGGAGA TAAAAGTCTT AAGAAATAAA ATGCTCAAAA TGGAGAAAGT CTTGCCGCTG 
ATTGGGTCTC CAGAACAGAA AACCCAAGGA AACCAAAAAT CAAAGGAACC CAGTGGTGCT 
GGTGCCAGTA GTAAGCCTGA GAATGATGTA AGTAAAGGCG ACAGCGGAGA CCTTGGTAAA 
GAAGAACGTG TCTCCCAGCT GATGAACGGA GACCCAGCTT TTAGACGTGG ACGTCTGCGT 
TGGATGAGGC AAGAGCAAAT TCGGTTCAAG AACCTGCAGC AGCAGGAAAT AACAAAGCAG 
CTTCGTCGGC AGAATGTACC TCATCGATTC ATCCCTCCGG AGAACCGGAA GCCGCGGTTT 
CCGTTTAAGA GCAACCCTAA ACACAGAAAC TCATGGAGTC CTGGGACACA CATCATCATC 
ACAGAAGATG AGGTAATAGA GCTTAGGATT CCCAAAGAGG AAGATGGAAG GAAAGGAAAT 
AAGCCGGAGA ACCAAGAAAA GGGTACTAGA GCAGCTTCTA AGGATCCCCA GTTACCATGG 
GGCCCTCAGG GCACTCGAAG TCAAGATCAC ATTCAGGTTA GCAAGCAGCA CATTAATAAT 
CAACAACAGC CACCTCAGTT ACGCTGGAGA AGCAATTCTC TCAATAACGG CCAACCGAAA 
AGTACGCGCT GCCAGGCATC TGCTTCCCCA GAAGTGCTCG CCTCCCACAG TGGCCACCCC 
ACCGCTGACC TGCAGACTTT TCAGGCGAAA CGCCACATGC ACCAGCACCG TCAAGCTTAC 
TGTAGTTATA ACACTGGAGC TCAGCTGGAG GGCAGTGCAG CCACTTCTTG TCAGAAGCAG 
ACTGACAGAC CCAACCACTG TAGCCAGTTT GTGACACCTC CGAGAATGAG GCGACAGTTC 
TCGGCACCCA ATCTCAAAAC TGGTCGGGAA ACCACAGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005194866.1
CDS	390..3851
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKCGLPSSGKKREPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKMKELCAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMASSKGGTDVDDLKVHIDKLEDILQEVKKQNNMKDEEIKVLRNKMLKMEKVLPLIGSPEQKTQGNQKSKEPSGAGASSKPENDVSKGDSGDLGKEERVSQLMNGDPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKPRFPFKSNPKHRNSWSPGTHIIITEDEVIELRIPKEEDGRKGNKPENQEKGTRAASKDPQLPWGPQGTRSQDHIQVSKQHINNQQQPPQLRWRSNSLNNGQPKSTRCQASASPEVLASHSGHPTADLQTFQAKRHMHQHRQAYCSYNTGAQLEGSAATSCQKQTDRPNHCSQFVTPPRMRRQFSAPNLKTGRETTV

Target ORF information:

RefSeq Version	XM_005194809.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005194809.3

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
GCGGATTCTG ATAGCGGGGA TGATTCTGAC AAGAGATCCT GCGAAGAGAG CTGGAAACTG 
ATTACTTCTC TGAGAGAAAA GCTGCCTCCC AGCAAGCTGC AAACCATTGT TAAAAAATGC 
GGCCTCCCAA GCAGTGGGAA GAAACGGGAA CCAATAAAAA TGTATCAGAT CCCCCAGAGA 
AGACGCCTGA GTAAAGACTC CAAGTGGGTC ACCATCTCAG ATCTCAAGAT TCAGGCTGTG 
AAAGAGATAT GCTACGAGGT TGCTCTCAAT GATTTTCGGC ATAGTCGGCA GGAGATTGAA 
GCCTTGGCCA TTGTTAAGAT GAAAGAACTT TGTGCCATGT ATGGGAAGAA AGATCCTAAT 
GAGCGGGACT CCTGGAGGGC AGTGGCCAGG GATGTCTGGG ACACCGTTGG TGTAGGGGAT 
GAGAAGATTG AGGACGTAAT GGCCAGTAGT AAAGGTGGGA CTGATGTGGA TGACCTCAAG 
GTTCATATAG ACAAGCTGGA AGATATTTTG CAAGAAGTCA AAAAGCAAAA TAACATGAAA 
GATGAGGAGA TAAAAGTCTT AAGAAATAAA ATGCTCAAAA TGGAGAAAGT CTTGCCGCTG 
ATTGGGTCTC CAGAACAGAA AACCCAAGGA AACCAAAAAT CAAAGGAACC CAGTGGTGCT 
GGTGCCAGTA GTAAGCCTGA GAATGATGTA AGTAAAGGCG ACAGCGGAGA CCTTGGTAAA 
GAAGAACGTG TCTCCCAGCT GATGAACGGA GACCCAGCTT TTAGACGTGG ACGTCTGCGT 
TGGATGAGGC AAGAGCAAAT TCGGTTCAAG AACCTGCAGC AGCAGGAAAT AACAAAGCAG 
CTTCGTCGGC AGAATGTACC TCATCGATTC ATCCCTCCGG AGAACCGGAA GCCGCGGTTT 
CCGTTTAAGA GCAACCCTAA ACACAGAAAC TCATGGAGTC CTGGGACACA CATCATCATC 
ACAGAAGATG AGGTAATAGA GCTTAGGATT CCCAAAGAGG AAGATGGAAG GAAAGGAAAT 
AAGCCGGAGA ACCAAGAAAA GGGTACTAGA GCAGCTTCTA AGGATCCCCA GTTACCATGG 
GGCCCTCAGG GCACTCGAAG TCAAGATCAC ATTCAGGTTA GCAAGCAGCA CATTAATAAT 
CAACAACAGC CACCTCAGTT ACGCTGGAGA AGCAATTCTC TCAATAACGG CCAACCGAAA 
AGTACGCGCT GCCAGGCATC TGCTTCCCCA GAAGTGCTCG CCTCCCACAG TGGCCACCCC 
ACCGCTGACC TGCAGACTTT TCAGGCGAAA CGCCACATGC ACCAGCACCG TCAAGCTTAC 
TGTAGTTATA ACACTGGAGC TCAGCTGGAG GGCAGTGCAG CCACTTCTTG TCAGAAGCAG 
ACTGACAGAC CCAACCACTG TAGCCAGTTT GTGACACCTC CGAGAATGAG GCGACAGTTC 
TCGGCACCCA ATCTCAAAAC TGGTCGGGAA ACCACAGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216998.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217055.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa27234
Clone ID Related Accession (Same CDS sequence)	XM_005194809.3 , XM_005216998.3
Accession Version	XM_005216998.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216998.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
GCGGATTCTG ATAGCGGGGA TGATTCTGAC AAGAGATCCT GCGAAGAGAG CTGGAAACTG 
ATTACTTCTC TGAGAGAAAA GCTGCCTCCC AGCAAGCTGC AAACCATTGT TAAAAAATGC 
GGCCTCCCAA GCAGTGGGAA GAAACGGGAA CCAATAAAAA TGTATCAGAT CCCCCAGAGA 
AGACGCCTGA GTAAAGACTC CAAGTGGGTC ACCATCTCAG ATCTCAAGAT TCAGGCTGTG 
AAAGAGATAT GCTACGAGGT TGCTCTCAAT GATTTTCGGC ATAGTCGGCA GGAGATTGAA 
GCCTTGGCCA TTGTTAAGAT GAAAGAACTT TGTGCCATGT ATGGGAAGAA AGATCCTAAT 
GAGCGGGACT CCTGGAGGGC AGTGGCCAGG GATGTCTGGG ACACCGTTGG TGTAGGGGAT 
GAGAAGATTG AGGACGTAAT GGCCAGTAGT AAAGGTGGGA CTGATGTGGA TGACCTCAAG 
GTTCATATAG ACAAGCTGGA AGATATTTTG CAAGAAGTCA AAAAGCAAAA TAACATGAAA 
GATGAGGAGA TAAAAGTCTT AAGAAATAAA ATGCTCAAAA TGGAGAAAGT CTTGCCGCTG 
ATTGGGTCTC CAGAACAGAA AACCCAAGGA AACCAAAAAT CAAAGGAACC CAGTGGTGCT 
GGTGCCAGTA GTAAGCCTGA GAATGATGTA AGTAAAGGCG ACAGCGGAGA CCTTGGTAAA 
GAAGAACGTG TCTCCCAGCT GATGAACGGA GACCCAGCTT TTAGACGTGG ACGTCTGCGT 
TGGATGAGGC AAGAGCAAAT TCGGTTCAAG AACCTGCAGC AGCAGGAAAT AACAAAGCAG 
CTTCGTCGGC AGAATGTACC TCATCGATTC ATCCCTCCGG AGAACCGGAA GCCGCGGTTT 
CCGTTTAAGA GCAACCCTAA ACACAGAAAC TCATGGAGTC CTGGGACACA CATCATCATC 
ACAGAAGATG AGGTAATAGA GCTTAGGATT CCCAAAGAGG AAGATGGAAG GAAAGGAAAT 
AAGCCGGAGA ACCAAGAAAA GGGTACTAGA GCAGCTTCTA AGGATCCCCA GTTACCATGG 
GGCCCTCAGG GCACTCGAAG TCAAGATCAC ATTCAGGTTA GCAAGCAGCA CATTAATAAT 
CAACAACAGC CACCTCAGTT ACGCTGGAGA AGCAATTCTC TCAATAACGG CCAACCGAAA 
AGTACGCGCT GCCAGGCATC TGCTTCCCCA GAAGTGCTCG CCTCCCACAG TGGCCACCCC 
ACCGCTGACC TGCAGACTTT TCAGGCGAAA CGCCACATGC ACCAGCACCG TCAAGCTTAC 
TGTAGTTATA ACACTGGAGC TCAGCTGGAG GGCAGTGCAG CCACTTCTTG TCAGAAGCAG 
ACTGACAGAC CCAACCACTG TAGCCAGTTT GTGACACCTC CGAGAATGAG GCGACAGTTC 
TCGGCACCCA ATCTCAAAAC TGGTCGGGAA ACCACAGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217055.1
CDS	390..3851
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKCGLPSSGKKREPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKMKELCAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMASSKGGTDVDDLKVHIDKLEDILQEVKKQNNMKDEEIKVLRNKMLKMEKVLPLIGSPEQKTQGNQKSKEPSGAGASSKPENDVSKGDSGDLGKEERVSQLMNGDPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKPRFPFKSNPKHRNSWSPGTHIIITEDEVIELRIPKEEDGRKGNKPENQEKGTRAASKDPQLPWGPQGTRSQDHIQVSKQHINNQQQPPQLRWRSNSLNNGQPKSTRCQASASPEVLASHSGHPTADLQTFQAKRHMHQHRQAYCSYNTGAQLEGSAATSCQKQTDRPNHCSQFVTPPRMRRQFSAPNLKTGRETTV

Target ORF information:

RefSeq Version	XM_005216998.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216998.3

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
GCGGATTCTG ATAGCGGGGA TGATTCTGAC AAGAGATCCT GCGAAGAGAG CTGGAAACTG 
ATTACTTCTC TGAGAGAAAA GCTGCCTCCC AGCAAGCTGC AAACCATTGT TAAAAAATGC 
GGCCTCCCAA GCAGTGGGAA GAAACGGGAA CCAATAAAAA TGTATCAGAT CCCCCAGAGA 
AGACGCCTGA GTAAAGACTC CAAGTGGGTC ACCATCTCAG ATCTCAAGAT TCAGGCTGTG 
AAAGAGATAT GCTACGAGGT TGCTCTCAAT GATTTTCGGC ATAGTCGGCA GGAGATTGAA 
GCCTTGGCCA TTGTTAAGAT GAAAGAACTT TGTGCCATGT ATGGGAAGAA AGATCCTAAT 
GAGCGGGACT CCTGGAGGGC AGTGGCCAGG GATGTCTGGG ACACCGTTGG TGTAGGGGAT 
GAGAAGATTG AGGACGTAAT GGCCAGTAGT AAAGGTGGGA CTGATGTGGA TGACCTCAAG 
GTTCATATAG ACAAGCTGGA AGATATTTTG CAAGAAGTCA AAAAGCAAAA TAACATGAAA 
GATGAGGAGA TAAAAGTCTT AAGAAATAAA ATGCTCAAAA TGGAGAAAGT CTTGCCGCTG 
ATTGGGTCTC CAGAACAGAA AACCCAAGGA AACCAAAAAT CAAAGGAACC CAGTGGTGCT 
GGTGCCAGTA GTAAGCCTGA GAATGATGTA AGTAAAGGCG ACAGCGGAGA CCTTGGTAAA 
GAAGAACGTG TCTCCCAGCT GATGAACGGA GACCCAGCTT TTAGACGTGG ACGTCTGCGT 
TGGATGAGGC AAGAGCAAAT TCGGTTCAAG AACCTGCAGC AGCAGGAAAT AACAAAGCAG 
CTTCGTCGGC AGAATGTACC TCATCGATTC ATCCCTCCGG AGAACCGGAA GCCGCGGTTT 
CCGTTTAAGA GCAACCCTAA ACACAGAAAC TCATGGAGTC CTGGGACACA CATCATCATC 
ACAGAAGATG AGGTAATAGA GCTTAGGATT CCCAAAGAGG AAGATGGAAG GAAAGGAAAT 
AAGCCGGAGA ACCAAGAAAA GGGTACTAGA GCAGCTTCTA AGGATCCCCA GTTACCATGG 
GGCCCTCAGG GCACTCGAAG TCAAGATCAC ATTCAGGTTA GCAAGCAGCA CATTAATAAT 
CAACAACAGC CACCTCAGTT ACGCTGGAGA AGCAATTCTC TCAATAACGG CCAACCGAAA 
AGTACGCGCT GCCAGGCATC TGCTTCCCCA GAAGTGCTCG CCTCCCACAG TGGCCACCCC 
ACCGCTGACC TGCAGACTTT TCAGGCGAAA CGCCACATGC ACCAGCACCG TCAAGCTTAC 
TGTAGTTATA ACACTGGAGC TCAGCTGGAG GGCAGTGCAG CCACTTCTTG TCAGAAGCAG 
ACTGACAGAC CCAACCACTG TAGCCAGTTT GTGACACCTC CGAGAATGAG GCGACAGTTC 
TCGGCACCCA ATCTCAAAAC TGGTCGGGAA ACCACAGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_010813295.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811597.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.

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CloneID	OBa60159
Clone ID Related Accession (Same CDS sequence)	XM_010813295.2 , XM_010823093.2
Accession Version	XM_010813295.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813295.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACGC GGATTCTGAT AGCGGGGATG ATTCTGACAA GAGATCCTGC 
GAAGAGAGCT GGAAACTGAT TACTTCTCTG AGAGAAAAGC TGCCTCCCAG CAAGCTGCAA 
ACCATTGTTA AAAAATGCGG CCTCCCAAGC AGTGGGAAGA AACGGGAACC AATAAAAATG 
TATCAGATCC CCCAGAGAAG ACGCCTGAGT AAAGACTCCA AGTGGGTCAC CATCTCAGAT 
CTCAAGATTC AGGCTGTGAA AGAGATATGC TACGAGGTTG CTCTCAATGA TTTTCGGCAT 
AGTCGGCAGG AGATTGAAGC CTTGGCCATT GTTAAGATGA AAGAACTTTG TGCCATGTAT 
GGGAAGAAAG ATCCTAATGA GCGGGACTCC TGGAGGGCAG TGGCCAGGGA TGTCTGGGAC 
ACCGTTGGTG TAGGGGATGA GAAGATTGAG GACGTAATGG CCAGTAGTAA AGGTGGGACT 
GATGTGGATG ACCTCAAGGT TCATATAGAC AAGCTGGAAG ATATTTTGCA AGAAGTCAAA 
AAGCAAAATA ACATGAAAGA TGAGGAGATA AAAGTCTTAA GAAATAAAAT GCTCAAAATG 
GAGAAAGTCT TGCCGCTGAT TGGGTCTCCA GAACAGAAAA CCCAAGGAAA CCAAAAATCA 
AAGGAACCCA GTGGTGCTGG TGCCAGTAGT AAGCCTGAGA ATGATGTAAG TAAAGGCGAC 
AGCGGAGACC TTGGTAAAGA AGAACGTGTC TCCCAGCTGA TGAACGGAGA CCCAGCTTTT 
AGACGTGGAC GTCTGCGTTG GATGAGGCAA GAGCAAATTC GGTTCAAGAA CCTGCAGCAG 
CAGGAAATAA CAAAGCAGCT TCGTCGGCAG AATGTACCTC ATCGATTCAT CCCTCCGGAG 
AACCGGAAGC CGCGGTTTCC GTTTAAGAGC AACCCTAAAC ACAGAAACTC ATGGAGTCCT 
GGGACACACA TCATCATCAC AGAAGATGAG GTAATAGAGC TTAGGATTCC CAAAGAGGAA 
GATGGAAGGA AAGGAAATAA GCCGGAGAAC CAAGAAAAGG GTACTAGAGC AGCTTCTAAG 
GATCCCCAGT TACCATGGGG CCCTCAGGGC ACTCGAAGTC AAGATCACAT TCAGGTTAGC 
AAGCAGCACA TTAATAATCA ACAACAGCCA CCTCAGTTAC GCTGGAGAAG CAATTCTCTC 
AATAACGGCC AACCGAAAAG TACGCGCTGC CAGGCATCTG CTTCCCCAGA AGTGCTCGCC 
TCCCACAGTG GCCACCCCAC CGCTGACCTG CAGACTTTTC AGGCGAAACG CCACATGCAC 
CAGCACCGTC AAGCTTACTG TAGTTATAAC ACTGGAGCTC AGCTGGAGGG CAGTGCAGCC 
ACTTCTTGTC AGAAGCAGAC TGACAGACCC AACCACTGTA GCCAGTTTGT GACACCTCCG 
AGAATGAGGC GACAGTTCTC GGCACCCAAT CTCAAAACTG GTCGGGAAAC CACAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811597.1
CDS	391..3990
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKCGLPSSGKKREPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKMKELCAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMASSKGGTDVDDLKVHIDKLEDILQEVKKQNNMKDEEIKVLRNKMLKMEKVLPLIGSPEQKTQGNQKSKEPSGAGASSKPENDVSKGDSGDLGKEERVSQLMNGDPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKPRFPFKSNPKHRNSWSPGTHIIITEDEVIELRIPKEEDGRKGNKPENQEKGTRAASKDPQLPWGPQGTRSQDHIQVSKQHINNQQQPPQLRWRSNSLNNGQPKSTRCQASASPEVLASHSGHPTADLQTFQAKRHMHQHRQAYCSYNTGAQLEGSAATSCQKQTDRPNHCSQFVTPPRMRRQFSAPNLKTGRETTV

Target ORF information:

RefSeq Version	XM_010813295.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010813295.2

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACGC GGATTCTGAT AGCGGGGATG ATTCTGACAA GAGATCCTGC 
GAAGAGAGCT GGAAACTGAT TACTTCTCTG AGAGAAAAGC TGCCTCCCAG CAAGCTGCAA 
ACCATTGTTA AAAAATGCGG CCTCCCAAGC AGTGGGAAGA AACGGGAACC AATAAAAATG 
TATCAGATCC CCCAGAGAAG ACGCCTGAGT AAAGACTCCA AGTGGGTCAC CATCTCAGAT 
CTCAAGATTC AGGCTGTGAA AGAGATATGC TACGAGGTTG CTCTCAATGA TTTTCGGCAT 
AGTCGGCAGG AGATTGAAGC CTTGGCCATT GTTAAGATGA AAGAACTTTG TGCCATGTAT 
GGGAAGAAAG ATCCTAATGA GCGGGACTCC TGGAGGGCAG TGGCCAGGGA TGTCTGGGAC 
ACCGTTGGTG TAGGGGATGA GAAGATTGAG GACGTAATGG CCAGTAGTAA AGGTGGGACT 
GATGTGGATG ACCTCAAGGT TCATATAGAC AAGCTGGAAG ATATTTTGCA AGAAGTCAAA 
AAGCAAAATA ACATGAAAGA TGAGGAGATA AAAGTCTTAA GAAATAAAAT GCTCAAAATG 
GAGAAAGTCT TGCCGCTGAT TGGGTCTCCA GAACAGAAAA CCCAAGGAAA CCAAAAATCA 
AAGGAACCCA GTGGTGCTGG TGCCAGTAGT AAGCCTGAGA ATGATGTAAG TAAAGGCGAC 
AGCGGAGACC TTGGTAAAGA AGAACGTGTC TCCCAGCTGA TGAACGGAGA CCCAGCTTTT 
AGACGTGGAC GTCTGCGTTG GATGAGGCAA GAGCAAATTC GGTTCAAGAA CCTGCAGCAG 
CAGGAAATAA CAAAGCAGCT TCGTCGGCAG AATGTACCTC ATCGATTCAT CCCTCCGGAG 
AACCGGAAGC CGCGGTTTCC GTTTAAGAGC AACCCTAAAC ACAGAAACTC ATGGAGTCCT 
GGGACACACA TCATCATCAC AGAAGATGAG GTAATAGAGC TTAGGATTCC CAAAGAGGAA 
GATGGAAGGA AAGGAAATAA GCCGGAGAAC CAAGAAAAGG GTACTAGAGC AGCTTCTAAG 
GATCCCCAGT TACCATGGGG CCCTCAGGGC ACTCGAAGTC AAGATCACAT TCAGGTTAGC 
AAGCAGCACA TTAATAATCA ACAACAGCCA CCTCAGTTAC GCTGGAGAAG CAATTCTCTC 
AATAACGGCC AACCGAAAAG TACGCGCTGC CAGGCATCTG CTTCCCCAGA AGTGCTCGCC 
TCCCACAGTG GCCACCCCAC CGCTGACCTG CAGACTTTTC AGGCGAAACG CCACATGCAC 
CAGCACCGTC AAGCTTACTG TAGTTATAAC ACTGGAGCTC AGCTGGAGGG CAGTGCAGCC 
ACTTCTTGTC AGAAGCAGAC TGACAGACCC AACCACTGTA GCCAGTTTGT GACACCTCCG 
AGAATGAGGC GACAGTTCTC GGCACCCAAT CTCAAAACTG GTCGGGAAAC CACAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_010823093.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821395.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.

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CloneID	OBa60159
Clone ID Related Accession (Same CDS sequence)	XM_010813295.2 , XM_010823093.2
Accession Version	XM_010823093.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823093.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACGC GGATTCTGAT AGCGGGGATG ATTCTGACAA GAGATCCTGC 
GAAGAGAGCT GGAAACTGAT TACTTCTCTG AGAGAAAAGC TGCCTCCCAG CAAGCTGCAA 
ACCATTGTTA AAAAATGCGG CCTCCCAAGC AGTGGGAAGA AACGGGAACC AATAAAAATG 
TATCAGATCC CCCAGAGAAG ACGCCTGAGT AAAGACTCCA AGTGGGTCAC CATCTCAGAT 
CTCAAGATTC AGGCTGTGAA AGAGATATGC TACGAGGTTG CTCTCAATGA TTTTCGGCAT 
AGTCGGCAGG AGATTGAAGC CTTGGCCATT GTTAAGATGA AAGAACTTTG TGCCATGTAT 
GGGAAGAAAG ATCCTAATGA GCGGGACTCC TGGAGGGCAG TGGCCAGGGA TGTCTGGGAC 
ACCGTTGGTG TAGGGGATGA GAAGATTGAG GACGTAATGG CCAGTAGTAA AGGTGGGACT 
GATGTGGATG ACCTCAAGGT TCATATAGAC AAGCTGGAAG ATATTTTGCA AGAAGTCAAA 
AAGCAAAATA ACATGAAAGA TGAGGAGATA AAAGTCTTAA GAAATAAAAT GCTCAAAATG 
GAGAAAGTCT TGCCGCTGAT TGGGTCTCCA GAACAGAAAA CCCAAGGAAA CCAAAAATCA 
AAGGAACCCA GTGGTGCTGG TGCCAGTAGT AAGCCTGAGA ATGATGTAAG TAAAGGCGAC 
AGCGGAGACC TTGGTAAAGA AGAACGTGTC TCCCAGCTGA TGAACGGAGA CCCAGCTTTT 
AGACGTGGAC GTCTGCGTTG GATGAGGCAA GAGCAAATTC GGTTCAAGAA CCTGCAGCAG 
CAGGAAATAA CAAAGCAGCT TCGTCGGCAG AATGTACCTC ATCGATTCAT CCCTCCGGAG 
AACCGGAAGC CGCGGTTTCC GTTTAAGAGC AACCCTAAAC ACAGAAACTC ATGGAGTCCT 
GGGACACACA TCATCATCAC AGAAGATGAG GTAATAGAGC TTAGGATTCC CAAAGAGGAA 
GATGGAAGGA AAGGAAATAA GCCGGAGAAC CAAGAAAAGG GTACTAGAGC AGCTTCTAAG 
GATCCCCAGT TACCATGGGG CCCTCAGGGC ACTCGAAGTC AAGATCACAT TCAGGTTAGC 
AAGCAGCACA TTAATAATCA ACAACAGCCA CCTCAGTTAC GCTGGAGAAG CAATTCTCTC 
AATAACGGCC AACCGAAAAG TACGCGCTGC CAGGCATCTG CTTCCCCAGA AGTGCTCGCC 
TCCCACAGTG GCCACCCCAC CGCTGACCTG CAGACTTTTC AGGCGAAACG CCACATGCAC 
CAGCACCGTC AAGCTTACTG TAGTTATAAC ACTGGAGCTC AGCTGGAGGG CAGTGCAGCC 
ACTTCTTGTC AGAAGCAGAC TGACAGACCC AACCACTGTA GCCAGTTTGT GACACCTCCG 
AGAATGAGGC GACAGTTCTC GGCACCCAAT CTCAAAACTG GTCGGGAAAC CACAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821395.1
CDS	391..3990
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKCGLPSSGKKREPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKMKELCAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMASSKGGTDVDDLKVHIDKLEDILQEVKKQNNMKDEEIKVLRNKMLKMEKVLPLIGSPEQKTQGNQKSKEPSGAGASSKPENDVSKGDSGDLGKEERVSQLMNGDPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKPRFPFKSNPKHRNSWSPGTHIIITEDEVIELRIPKEEDGRKGNKPENQEKGTRAASKDPQLPWGPQGTRSQDHIQVSKQHINNQQQPPQLRWRSNSLNNGQPKSTRCQASASPEVLASHSGHPTADLQTFQAKRHMHQHRQAYCSYNTGAQLEGSAATSCQKQTDRPNHCSQFVTPPRMRRQFSAPNLKTGRETTV

Target ORF information:

RefSeq Version	XM_010823093.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823093.2

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACGC GGATTCTGAT AGCGGGGATG ATTCTGACAA GAGATCCTGC 
GAAGAGAGCT GGAAACTGAT TACTTCTCTG AGAGAAAAGC TGCCTCCCAG CAAGCTGCAA 
ACCATTGTTA AAAAATGCGG CCTCCCAAGC AGTGGGAAGA AACGGGAACC AATAAAAATG 
TATCAGATCC CCCAGAGAAG ACGCCTGAGT AAAGACTCCA AGTGGGTCAC CATCTCAGAT 
CTCAAGATTC AGGCTGTGAA AGAGATATGC TACGAGGTTG CTCTCAATGA TTTTCGGCAT 
AGTCGGCAGG AGATTGAAGC CTTGGCCATT GTTAAGATGA AAGAACTTTG TGCCATGTAT 
GGGAAGAAAG ATCCTAATGA GCGGGACTCC TGGAGGGCAG TGGCCAGGGA TGTCTGGGAC 
ACCGTTGGTG TAGGGGATGA GAAGATTGAG GACGTAATGG CCAGTAGTAA AGGTGGGACT 
GATGTGGATG ACCTCAAGGT TCATATAGAC AAGCTGGAAG ATATTTTGCA AGAAGTCAAA 
AAGCAAAATA ACATGAAAGA TGAGGAGATA AAAGTCTTAA GAAATAAAAT GCTCAAAATG 
GAGAAAGTCT TGCCGCTGAT TGGGTCTCCA GAACAGAAAA CCCAAGGAAA CCAAAAATCA 
AAGGAACCCA GTGGTGCTGG TGCCAGTAGT AAGCCTGAGA ATGATGTAAG TAAAGGCGAC 
AGCGGAGACC TTGGTAAAGA AGAACGTGTC TCCCAGCTGA TGAACGGAGA CCCAGCTTTT 
AGACGTGGAC GTCTGCGTTG GATGAGGCAA GAGCAAATTC GGTTCAAGAA CCTGCAGCAG 
CAGGAAATAA CAAAGCAGCT TCGTCGGCAG AATGTACCTC ATCGATTCAT CCCTCCGGAG 
AACCGGAAGC CGCGGTTTCC GTTTAAGAGC AACCCTAAAC ACAGAAACTC ATGGAGTCCT 
GGGACACACA TCATCATCAC AGAAGATGAG GTAATAGAGC TTAGGATTCC CAAAGAGGAA 
GATGGAAGGA AAGGAAATAA GCCGGAGAAC CAAGAAAAGG GTACTAGAGC AGCTTCTAAG 
GATCCCCAGT TACCATGGGG CCCTCAGGGC ACTCGAAGTC AAGATCACAT TCAGGTTAGC 
AAGCAGCACA TTAATAATCA ACAACAGCCA CCTCAGTTAC GCTGGAGAAG CAATTCTCTC 
AATAACGGCC AACCGAAAAG TACGCGCTGC CAGGCATCTG CTTCCCCAGA AGTGCTCGCC 
TCCCACAGTG GCCACCCCAC CGCTGACCTG CAGACTTTTC AGGCGAAACG CCACATGCAC 
CAGCACCGTC AAGCTTACTG TAGTTATAAC ACTGGAGCTC AGCTGGAGGG CAGTGCAGCC 
ACTTCTTGTC AGAAGCAGAC TGACAGACCC AACCACTGTA GCCAGTTTGT GACACCTCCG 
AGAATGAGGC GACAGTTCTC GGCACCCAAT CTCAAAACTG GTCGGGAAAC CACAGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_024976729.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832497.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa223919
Clone ID Related Accession (Same CDS sequence)	XM_024976729.1 , XM_005216992.4
Accession Version	XM_024976729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGATGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACATTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTGCAG AATATTGCAG TGGAGGTTAC GGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGCCAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832497.1
CDS	1..5451
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_024976729.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976729.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGATGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACATTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTGCAG AATATTGCAG TGGAGGTTAC GGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGCCAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216992.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217049.2 Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.

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CloneID	OBa223919
Clone ID Related Accession (Same CDS sequence)	XM_024976729.1 , XM_005216992.4
Accession Version	XM_005216992.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216992.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGATGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACATTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTGCAG AATATTGCAG TGGAGGTTAC GGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGCCAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217049.2
CDS	381..5831
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_005216992.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216992.4

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGATGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACATTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTGCAG AATATTGCAG TGGAGGTTAC GGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGCCAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_024976730.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832498.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.

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CloneID	OBa223920
Clone ID Related Accession (Same CDS sequence)	XM_024976730.1
Accession Version	XM_024976730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TATTGATCCA TTGATCGATG ATTACTCGGG AAGTGGAGGC 
AAATATCTGA AAGATTTTCA GAACAATAAG CATAGGTACT TGCTAGCCTC TGAGAATCAA 
CGTCCTGGCA ATTTTTCCAC AGCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGATGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACATT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTGC AGAATATTGC AGTGGAGGTT ACGGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGCC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832498.1
CDS	380..5812
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_024976730.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976730.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TATTGATCCA TTGATCGATG ATTACTCGGG AAGTGGAGGC 
AAATATCTGA AAGATTTTCA GAACAATAAG CATAGGTACT TGCTAGCCTC TGAGAATCAA 
CGTCCTGGCA ATTTTTCCAC AGCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGATGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACATT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTGC AGAATATTGC AGTGGAGGTT ACGGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGCC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216993.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217050.2 Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.

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CloneID	OBa223921
Clone ID Related Accession (Same CDS sequence)	XM_005216993.4
Accession Version	XM_005216993.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216993.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGATGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACATT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTGC AGAATATTGC AGTGGAGGTT ACGGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGCC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217050.2
CDS	380..5752
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKSSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_005216993.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216993.4

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGATGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACATT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTGC AGAATATTGC AGTGGAGGTT ACGGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGCC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_002694045.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002694091.4 Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.

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CloneID	OBa223922
Clone ID Related Accession (Same CDS sequence)	XM_002694045.6
Accession Version	XM_002694045.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002694045.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGATGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACATTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTGCAG AATATTGCAG TGGAGGTTAC GGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGCCAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002694091.4
CDS	380..5710
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_002694045.6
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002694045.6

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGATGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACATTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTGCAG AATATTGCAG TGGAGGTTAC GGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGCCAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216995.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217052.2 Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.

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CloneID	OBa223923
Clone ID Related Accession (Same CDS sequence)	XM_005216995.4
Accession Version	XM_005216995.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216995.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGATGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACATT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTGC AGAATATTGC AGTGGAGGTT ACGGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGCC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217052.2
CDS	380..5692
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_005216995.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216995.4

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGATGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACATT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTGC AGAATATTGC AGTGGAGGTT ACGGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGCC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_024976731.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832499.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.

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CloneID	OBa223924
Clone ID Related Accession (Same CDS sequence)	XM_024976731.1
Accession Version	XM_024976731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACATTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTGCA GAATATTGCA GTGGAGGTTA CGGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGT TCCAGCCACC AAGTTAAATA CCATGAGCAA AACCAGCCTT 
GGCCAGAGCA TGAGCAAGTA TGATCTTCTG GTTTGGTTTG AGATCAGTGA ACTGGAGCCT 
ACGGGAGAGT ATATCCCAGC CGTGGTGGAC CATACAGCAG GCTTGCCCTG CCAGGGGACG 
TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG ATCACAGTGA CCATCATCCA TGAGAAGGGG 
AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG CTGGTGGTAG GCCGAATCAG GAATAAGGCT 
GAGGTGGATG AAGCTGCAGT GGACGCCATC CTCTCCCTCA ATATCATCTC TGCCAAGTAC 
CTGAAGTCGT CCCACAACTG TAGCAGGACC TTCTATCGTT TCGAGGCCGT CTGGGATAGT 
TCCCTCCATA ACTCCCTCCT TCTGAACCGA GTGACACCCT ACGGAGAAAA GATCTACATG 
ACCCTGTCGG CCTACCTGGA GCTGGATCAC TGCATCCAGC CGGCCGTCAT CACCAAGGAC 
GTGTGCATGG TCTTCTACTC CCGGGACGCC AAGATCTCGC CGCCACGCTC GCTGCGCAGC 
CTCTTTGGCA GCGGCTATTC GAAGTCACCA GACTCCAATC GAGTCACTGG AATTTATGAA 
CTCAGTTTAT GCAAAATGGC AGACACAGGA AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA 
ATCTTGGATA CCTCAGTGGC GTATGTGCGG GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC 
CGGGGAGACA GCCTCATCCT AGAGCACCAG TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC 
GAGGTGGAGA AAACCCGCCA CTTTCTGCTG CTCCGTGACA GACTGGGTGA CAGCATCCCC 
AAATCCCTGA GTGACTCGTT GTCCCCCAGC CTCAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CTCAGATCTC AACCACCACC TTTGAAAGTG CCATCACACC CAGCGAGAGT 
AGTGGCTACG ACTCAGCAGA CATTGAAAGC CTGGTGGATC GAGAGAAAGA GCTGGCTACA 
AAGTGCCTGC AACTTCTCAC CCACACTTTC AACCGAGAAT TCAGCCAGGT GCACGGCAGC 
ATCAGTGACT GCAAGCTGGC TGACCTCTCC CCCGTCGGCC GGGACCCCTC TGTGTCCAGT 
CTGAGCAGTT CCACCCTCAC GCCATCCTCT ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC 
AACTCCCTGG ATCAGAAGAC CCCAGAAGCC AATTCCCGGG CCTCTAGTCC CTGCCCAGAA 
TTTGAACAGT TTCAGATTGT CCCACCTGTG GAAACACCCT ATTTGGCCCG AGCAGGGAAA 
AATGAATTTC TCAATCTTGT TCCAGATATT GAAGAAATTA GACCAGGCTC AGTGGTCTCC 
AAGAAAGGAT ATCTGCATTT CAAGGAGCCC CTGTCCAGCA ACTGGGCTAA ACATTTCGTC 
GTGGTCCGTC GCCCTTACGT CTTCATCTAT AACAGCGACA AAGACCCTGT GGAGCGGGGC 
ATCATTAACC TGTCCACAGC CCAGGTGGAG TATAGCGAGG ACCAGCAGGC CATGGTGAAG 
ACACCGAACA CCTTTGCTGT GTGCACCAAG CACCGTGGGG TCCTTTTGCA GGCTCTCAAC 
GACAAAGACG TGAACGACTG GTTGTACGCC TTTAACCCGC TCCTTGCTGG CACAATACGG 
TCAAAACTCT CCCGCAGATG CCCGAGCCAA GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832499.1
CDS	380..5581
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_024976731.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976731.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACATTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTGCA GAATATTGCA GTGGAGGTTA CGGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGT TCCAGCCACC AAGTTAAATA CCATGAGCAA AACCAGCCTT 
GGCCAGAGCA TGAGCAAGTA TGATCTTCTG GTTTGGTTTG AGATCAGTGA ACTGGAGCCT 
ACGGGAGAGT ATATCCCAGC CGTGGTGGAC CATACAGCAG GCTTGCCCTG CCAGGGGACG 
TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG ATCACAGTGA CCATCATCCA TGAGAAGGGG 
AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG CTGGTGGTAG GCCGAATCAG GAATAAGGCT 
GAGGTGGATG AAGCTGCAGT GGACGCCATC CTCTCCCTCA ATATCATCTC TGCCAAGTAC 
CTGAAGTCGT CCCACAACTG TAGCAGGACC TTCTATCGTT TCGAGGCCGT CTGGGATAGT 
TCCCTCCATA ACTCCCTCCT TCTGAACCGA GTGACACCCT ACGGAGAAAA GATCTACATG 
ACCCTGTCGG CCTACCTGGA GCTGGATCAC TGCATCCAGC CGGCCGTCAT CACCAAGGAC 
GTGTGCATGG TCTTCTACTC CCGGGACGCC AAGATCTCGC CGCCACGCTC GCTGCGCAGC 
CTCTTTGGCA GCGGCTATTC GAAGTCACCA GACTCCAATC GAGTCACTGG AATTTATGAA 
CTCAGTTTAT GCAAAATGGC AGACACAGGA AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA 
ATCTTGGATA CCTCAGTGGC GTATGTGCGG GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC 
CGGGGAGACA GCCTCATCCT AGAGCACCAG TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC 
GAGGTGGAGA AAACCCGCCA CTTTCTGCTG CTCCGTGACA GACTGGGTGA CAGCATCCCC 
AAATCCCTGA GTGACTCGTT GTCCCCCAGC CTCAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CTCAGATCTC AACCACCACC TTTGAAAGTG CCATCACACC CAGCGAGAGT 
AGTGGCTACG ACTCAGCAGA CATTGAAAGC CTGGTGGATC GAGAGAAAGA GCTGGCTACA 
AAGTGCCTGC AACTTCTCAC CCACACTTTC AACCGAGAAT TCAGCCAGGT GCACGGCAGC 
ATCAGTGACT GCAAGCTGGC TGACCTCTCC CCCGTCGGCC GGGACCCCTC TGTGTCCAGT 
CTGAGCAGTT CCACCCTCAC GCCATCCTCT ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC 
AACTCCCTGG ATCAGAAGAC CCCAGAAGCC AATTCCCGGG CCTCTAGTCC CTGCCCAGAA 
TTTGAACAGT TTCAGATTGT CCCACCTGTG GAAACACCCT ATTTGGCCCG AGCAGGGAAA 
AATGAATTTC TCAATCTTGT TCCAGATATT GAAGAAATTA GACCAGGCTC AGTGGTCTCC 
AAGAAAGGAT ATCTGCATTT CAAGGAGCCC CTGTCCAGCA ACTGGGCTAA ACATTTCGTC 
GTGGTCCGTC GCCCTTACGT CTTCATCTAT AACAGCGACA AAGACCCTGT GGAGCGGGGC 
ATCATTAACC TGTCCACAGC CCAGGTGGAG TATAGCGAGG ACCAGCAGGC CATGGTGAAG 
ACACCGAACA CCTTTGCTGT GTGCACCAAG CACCGTGGGG TCCTTTTGCA GGCTCTCAAC 
GACAAAGACG TGAACGACTG GTTGTACGCC TTTAACCCGC TCCTTGCTGG CACAATACGG 
TCAAAACTCT CCCGCAGATG CCCGAGCCAA GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216997.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217054.2 Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.

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CloneID	OBa223925
Clone ID Related Accession (Same CDS sequence)	XM_005216997.4
Accession Version	XM_005216997.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216997.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACATTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTGCA GAATATTGCA GTGGAGGTTA CGGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGT TCCAGCCACC AAGTTAAATA CCATGAGCAA AACCAGCCTT 
GGCCAGAGCA TGAGCAAGTA TGATCTTCTG GTTTGGTTTG AGATCAGTGA ACTGGAGCCT 
ACGGGAGAGT ATATCCCAGC CGTGGTGGAC CATACAGCAG GCTTGCCCTG CCAGGGGACG 
TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG ATCACAGTGA CCATCATCCA TGAGAAGGGG 
AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG CTGGTGGTAG GCCGAATCAG GAATAAGGCT 
GAGGTGGATG AAGCTGCAGT GGACGCCATC CTCTCCCTCA ATATCATCTC TGCCAAGTAC 
CTGAAGTCGT CCCACAACTG TAGCAGGACC TTCTATCGTT TCGAGGCCGT CTGGGATAGT 
TCCCTCCATA ACTCCCTCCT TCTGAACCGA GTGACACCCT ACGGAGAAAA GATCTACATG 
ACCCTGTCGG CCTACCTGGA GCTGGATCAC TGCATCCAGC CGGCCGTCAT CACCAAGGAC 
GTGTGCATGG TCTTCTACTC CCGGGACGCC AAGATCTCGC CGCCACGCTC GCTGCGCAGC 
CTCTTTGGCA GCGGCTATTC GAAGTCACCA GACTCCAATC GAGTCACTGG AATTTATGAA 
CTCAGTTTAT GCAAAATGGC AGACACAGGA AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA 
ATCTTGGATA CCTCAGTGGC GTATGTGCGG GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC 
CGGGGAGACA GCCTCATCCT AGAGCACCAG TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC 
GAGGTGGAGA AAACCCGCCA CTTTCTGCTG CTCCGTGACA GACTGGGTGA CAGCATCCCC 
AAATCCCTGA GTGACTCGTT GTCCCCCAGC CTCAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CTCAGATCTC AACCACCACC TTTGAAAGTG CCATCACACC CAGCGAGAGT 
AGTGGCTACG ACTCAGCAGA CATTGAAAGC CTGGTGGATC GAGAGAAAGA GCTGGCTACA 
AAGTGCCTGC AACTTCTCAC CCACACTTTC AACCGAGAAT TCAGCCAGGT GCACGGCAGC 
ATCAGTGACT GCAAGCTGGC TGACCTCTCC CCCGTCGGCC GGGACCCCTC TGTGTCCAGT 
CTGAGCAGTT CCACCCTCAC GCCATCCTCT ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC 
AACTCCCTGG ATCAGAAGAC CCCAGAAGCC AATTCCCGGG CCTCTAGTCC CTGCCCAGAA 
TTTGAACAGT TTCAGATTGT CCCACCTGTG GAAACACCCT ATTTGGCCCG AGCAGGGAAA 
AATGAATTTC TCAATCTTGT TCCAGATATT GAAGAAATTA GACCAGGCTC AGTGGTCTCC 
AAGAAAGGAT ATCTGCATTT CAAGGAGCCC CTGTCCAGCA ACTGGGCTAA ACATTTCGTC 
GTGGTCCGTC GCCCTTACGT CTTCATCTAT AACAGCGACA AAGACCCTGT GGAGCGGGGC 
ATCATTAACC TGTCCACAGC CCAGGTGGAG TATAGCGAGG ACCAGCAGGC CATGGTGAAG 
ACACCGAACA CCTTTGCTGT GTGCACCAAG CACCGTGGGG TCCTTTTGCA GGCTCTCAAC 
GACAAAGACG TGAACGACTG GTTGTACGCC TTTAACCCGC TCCTTGCTGG CACAATACGG 
TCAAAACTCT CCCGCAGATG CCCGAGCCAA GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217054.2
CDS	380..5461
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_005216997.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216997.4

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTGTTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTTTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACATTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTGCA GAATATTGCA GTGGAGGTTA CGGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGT TCCAGCCACC AAGTTAAATA CCATGAGCAA AACCAGCCTT 
GGCCAGAGCA TGAGCAAGTA TGATCTTCTG GTTTGGTTTG AGATCAGTGA ACTGGAGCCT 
ACGGGAGAGT ATATCCCAGC CGTGGTGGAC CATACAGCAG GCTTGCCCTG CCAGGGGACG 
TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG ATCACAGTGA CCATCATCCA TGAGAAGGGG 
AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG CTGGTGGTAG GCCGAATCAG GAATAAGGCT 
GAGGTGGATG AAGCTGCAGT GGACGCCATC CTCTCCCTCA ATATCATCTC TGCCAAGTAC 
CTGAAGTCGT CCCACAACTG TAGCAGGACC TTCTATCGTT TCGAGGCCGT CTGGGATAGT 
TCCCTCCATA ACTCCCTCCT TCTGAACCGA GTGACACCCT ACGGAGAAAA GATCTACATG 
ACCCTGTCGG CCTACCTGGA GCTGGATCAC TGCATCCAGC CGGCCGTCAT CACCAAGGAC 
GTGTGCATGG TCTTCTACTC CCGGGACGCC AAGATCTCGC CGCCACGCTC GCTGCGCAGC 
CTCTTTGGCA GCGGCTATTC GAAGTCACCA GACTCCAATC GAGTCACTGG AATTTATGAA 
CTCAGTTTAT GCAAAATGGC AGACACAGGA AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA 
ATCTTGGATA CCTCAGTGGC GTATGTGCGG GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC 
CGGGGAGACA GCCTCATCCT AGAGCACCAG TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC 
GAGGTGGAGA AAACCCGCCA CTTTCTGCTG CTCCGTGACA GACTGGGTGA CAGCATCCCC 
AAATCCCTGA GTGACTCGTT GTCCCCCAGC CTCAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CTCAGATCTC AACCACCACC TTTGAAAGTG CCATCACACC CAGCGAGAGT 
AGTGGCTACG ACTCAGCAGA CATTGAAAGC CTGGTGGATC GAGAGAAAGA GCTGGCTACA 
AAGTGCCTGC AACTTCTCAC CCACACTTTC AACCGAGAAT TCAGCCAGGT GCACGGCAGC 
ATCAGTGACT GCAAGCTGGC TGACCTCTCC CCCGTCGGCC GGGACCCCTC TGTGTCCAGT 
CTGAGCAGTT CCACCCTCAC GCCATCCTCT ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC 
AACTCCCTGG ATCAGAAGAC CCCAGAAGCC AATTCCCGGG CCTCTAGTCC CTGCCCAGAA 
TTTGAACAGT TTCAGATTGT CCCACCTGTG GAAACACCCT ATTTGGCCCG AGCAGGGAAA 
AATGAATTTC TCAATCTTGT TCCAGATATT GAAGAAATTA GACCAGGCTC AGTGGTCTCC 
AAGAAAGGAT ATCTGCATTT CAAGGAGCCC CTGTCCAGCA ACTGGGCTAA ACATTTCGTC 
GTGGTCCGTC GCCCTTACGT CTTCATCTAT AACAGCGACA AAGACCCTGT GGAGCGGGGC 
ATCATTAACC TGTCCACAGC CCAGGTGGAG TATAGCGAGG ACCAGCAGGC CATGGTGAAG 
ACACCGAACA CCTTTGCTGT GTGCACCAAG CACCGTGGGG TCCTTTTGCA GGCTCTCAAC 
GACAAAGACG TGAACGACTG GTTGTACGCC TTTAACCCGC TCCTTGCTGG CACAATACGG 
TCAAAACTCT CCCGCAGATG CCCGAGCCAA GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_024976732.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832500.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.

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CloneID	OBa223926
Clone ID Related Accession (Same CDS sequence)	XM_024976732.1
Accession Version	XM_024976732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGGGC CTTTGTTTAC CTGAGCAACC TGCTGTACCC CGTGCCCCTG 
ATTCACAGGG TGGCCATCGT CAGTGAGAAG GGAGAGGTGC GTGGATTTCT GCGTGTGGCT 
GTGCAGGCCA TTGCAGCGGA TGAAGAAGCT CCTGATTATG GCTCTGGAAT TCGACAGTCA 
GGAACAGCTA AAATATCTTT TGATAACGAG TACTTTAATC AGAGTGACAT TTCTTCGGTT 
GCATTGACCC GTTCTGGTCT GTCCTTGGAG GAGCTGAGGA TTGTGGAAGG ACAGGGTCAG 
AGTTCTGAGG TCATCACTCC TCCAGAAGAA GTCAACCGAA TGAATGATCT GGATCTGAAG 
TCAAGCACTT TGCTGGATGG TAAGATGATG ATGGAGGGAT TTTCTGAAGA GATCGGCAAC 
CACCTAAAGC TGGGCAGTGC ATTCACTTTC CGGGTCACAG TGCTGCAGGC CAGTGGAATC 
CTCCCAGAGT ATGCAGACAT CTTCTGTCAG TTCAACTTTT TACATCGGCA TGATGAAGCA 
TTCTCCACTG AACCCCTCAA GAACAACGGC AGAGGAAGTC CTCTGGGCTT TTACCATGTG 
CAGAATATTG CAGTGGAGGT TACGGAATCA TTCGTGGACT ATATCAAAAC CAAGCCGATT 
GTGTTTGAAG TCTTTGGGCA TTATCAGCAG CACCCACTTC ACCTGCAAGG ACAGGAACTT 
CACAGTCCAC CCCAGCCGTC TCGACGATTC TTCCCTCCGC CCATGCCGCT ATCCAAGCCA 
GTTCCAGCCA CCAAGTTAAA TACCATGAGC AAAACCAGCC TTGGCCAGAG CATGAGCAAG 
TATGATCTTC TGGTTTGGTT TGAGATCAGT GAACTGGAGC CTACGGGAGA GTATATCCCA 
GCCGTGGTGG ACCATACAGC AGGCTTGCCC TGCCAGGGGA CGTTTTTGCT TCACCAGGGC 
ATCCAGCGGA GGATCACAGT GACCATCATC CATGAGAAGG GGAGTGAGCT CCACTGGAAG 
GATGTTCGTG AGCTGGTGGT AGGCCGAATC AGGAATAAGG CTGAGGTGGA TGAAGCTGCA 
GTGGACGCCA TCCTCTCCCT CAATATCATC TCTGCCAAGT ACCTGAAGTC GTCCCACAAC 
TGTAGCAGGA CCTTCTATCG TTTCGAGGCC GTCTGGGATA GTTCCCTCCA TAACTCCCTC 
CTTCTGAACC GAGTGACACC CTACGGAGAA AAGATCTACA TGACCCTGTC GGCCTACCTG 
GAGCTGGATC ACTGCATCCA GCCGGCCGTC ATCACCAAGG ACGTGTGCAT GGTCTTCTAC 
TCCCGGGACG CCAAGATCTC GCCGCCACGC TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT 
TCGAAGTCAC CAGACTCCAA TCGAGTCACT GGAATTTATG AACTCAGTTT ATGCAAAATG 
GCAGACACAG GAAGTCCAGG CATGCAGCGG AGAAGAAGGA AAATCTTGGA TACCTCAGTG 
GCGTATGTGC GGGGGGAGGA GAACTTGGCT GGCTGGCGGC CCCGGGGAGA CAGCCTCATC 
CTAGAGCACC AGTGGGAGTT GGAGAAGCTG GAGCTCCTGC ACGAGGTGGA GAAAACCCGC 
CACTTTCTGC TGCTCCGTGA CAGACTGGGT GACAGCATCC CCAAATCCCT GAGTGACTCG 
TTGTCCCCCA GCCTCAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCTCAGATC 
TCAACCACCA CCTTTGAAAG TGCCATCACA CCCAGCGAGA GTAGTGGCTA CGACTCAGCA 
GACATTGAAA GCCTGGTGGA TCGAGAGAAA GAGCTGGCTA CAAAGTGCCT GCAACTTCTC 
ACCCACACTT TCAACCGAGA ATTCAGCCAG GTGCACGGCA GCATCAGTGA CTGCAAGCTG 
GCTGACCTCT CCCCCGTCGG CCGGGACCCC TCTGTGTCCA GTCTGAGCAG TTCCACCCTC 
ACGCCATCCT CTACCTGCCC CTCTCTGGTG GATGCTAGGA GCAACTCCCT GGATCAGAAG 
ACCCCAGAAG CCAATTCCCG GGCCTCTAGT CCCTGCCCAG AATTTGAACA GTTTCAGATT 
GTCCCACCTG TGGAAACACC CTATTTGGCC CGAGCAGGGA AAAATGAATT TCTCAATCTT 
GTTCCAGATA TTGAAGAAAT TAGACCAGGC TCAGTGGTCT CCAAGAAAGG ATATCTGCAT 
TTCAAGGAGC CCCTGTCCAG CAACTGGGCT AAACATTTCG TCGTGGTCCG TCGCCCTTAC 
GTCTTCATCT ATAACAGCGA CAAAGACCCT GTGGAGCGGG GCATCATTAA CCTGTCCACA 
GCCCAGGTGG AGTATAGCGA GGACCAGCAG GCCATGGTGA AGACACCGAA CACCTTTGCT 
GTGTGCACCA AGCACCGTGG GGTCCTTTTG CAGGCTCTCA ACGACAAAGA CGTGAACGAC 
TGGTTGTACG CCTTTAACCC GCTCCTTGCT GGCACAATAC GGTCAAAACT CTCCCGCAGA 
TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832500.1
CDS	353..5416
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDISSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_024976732.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976732.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTGTTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTTT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGGGC CTTTGTTTAC CTGAGCAACC TGCTGTACCC CGTGCCCCTG 
ATTCACAGGG TGGCCATCGT CAGTGAGAAG GGAGAGGTGC GTGGATTTCT GCGTGTGGCT 
GTGCAGGCCA TTGCAGCGGA TGAAGAAGCT CCTGATTATG GCTCTGGAAT TCGACAGTCA 
GGAACAGCTA AAATATCTTT TGATAACGAG TACTTTAATC AGAGTGACAT TTCTTCGGTT 
GCATTGACCC GTTCTGGTCT GTCCTTGGAG GAGCTGAGGA TTGTGGAAGG ACAGGGTCAG 
AGTTCTGAGG TCATCACTCC TCCAGAAGAA GTCAACCGAA TGAATGATCT GGATCTGAAG 
TCAAGCACTT TGCTGGATGG TAAGATGATG ATGGAGGGAT TTTCTGAAGA GATCGGCAAC 
CACCTAAAGC TGGGCAGTGC ATTCACTTTC CGGGTCACAG TGCTGCAGGC CAGTGGAATC 
CTCCCAGAGT ATGCAGACAT CTTCTGTCAG TTCAACTTTT TACATCGGCA TGATGAAGCA 
TTCTCCACTG AACCCCTCAA GAACAACGGC AGAGGAAGTC CTCTGGGCTT TTACCATGTG 
CAGAATATTG CAGTGGAGGT TACGGAATCA TTCGTGGACT ATATCAAAAC CAAGCCGATT 
GTGTTTGAAG TCTTTGGGCA TTATCAGCAG CACCCACTTC ACCTGCAAGG ACAGGAACTT 
CACAGTCCAC CCCAGCCGTC TCGACGATTC TTCCCTCCGC CCATGCCGCT ATCCAAGCCA 
GTTCCAGCCA CCAAGTTAAA TACCATGAGC AAAACCAGCC TTGGCCAGAG CATGAGCAAG 
TATGATCTTC TGGTTTGGTT TGAGATCAGT GAACTGGAGC CTACGGGAGA GTATATCCCA 
GCCGTGGTGG ACCATACAGC AGGCTTGCCC TGCCAGGGGA CGTTTTTGCT TCACCAGGGC 
ATCCAGCGGA GGATCACAGT GACCATCATC CATGAGAAGG GGAGTGAGCT CCACTGGAAG 
GATGTTCGTG AGCTGGTGGT AGGCCGAATC AGGAATAAGG CTGAGGTGGA TGAAGCTGCA 
GTGGACGCCA TCCTCTCCCT CAATATCATC TCTGCCAAGT ACCTGAAGTC GTCCCACAAC 
TGTAGCAGGA CCTTCTATCG TTTCGAGGCC GTCTGGGATA GTTCCCTCCA TAACTCCCTC 
CTTCTGAACC GAGTGACACC CTACGGAGAA AAGATCTACA TGACCCTGTC GGCCTACCTG 
GAGCTGGATC ACTGCATCCA GCCGGCCGTC ATCACCAAGG ACGTGTGCAT GGTCTTCTAC 
TCCCGGGACG CCAAGATCTC GCCGCCACGC TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT 
TCGAAGTCAC CAGACTCCAA TCGAGTCACT GGAATTTATG AACTCAGTTT ATGCAAAATG 
GCAGACACAG GAAGTCCAGG CATGCAGCGG AGAAGAAGGA AAATCTTGGA TACCTCAGTG 
GCGTATGTGC GGGGGGAGGA GAACTTGGCT GGCTGGCGGC CCCGGGGAGA CAGCCTCATC 
CTAGAGCACC AGTGGGAGTT GGAGAAGCTG GAGCTCCTGC ACGAGGTGGA GAAAACCCGC 
CACTTTCTGC TGCTCCGTGA CAGACTGGGT GACAGCATCC CCAAATCCCT GAGTGACTCG 
TTGTCCCCCA GCCTCAGCAG CGGGACCCTC AGCACCTCCA CCAGCATCTC CTCTCAGATC 
TCAACCACCA CCTTTGAAAG TGCCATCACA CCCAGCGAGA GTAGTGGCTA CGACTCAGCA 
GACATTGAAA GCCTGGTGGA TCGAGAGAAA GAGCTGGCTA CAAAGTGCCT GCAACTTCTC 
ACCCACACTT TCAACCGAGA ATTCAGCCAG GTGCACGGCA GCATCAGTGA CTGCAAGCTG 
GCTGACCTCT CCCCCGTCGG CCGGGACCCC TCTGTGTCCA GTCTGAGCAG TTCCACCCTC 
ACGCCATCCT CTACCTGCCC CTCTCTGGTG GATGCTAGGA GCAACTCCCT GGATCAGAAG 
ACCCCAGAAG CCAATTCCCG GGCCTCTAGT CCCTGCCCAG AATTTGAACA GTTTCAGATT 
GTCCCACCTG TGGAAACACC CTATTTGGCC CGAGCAGGGA AAAATGAATT TCTCAATCTT 
GTTCCAGATA TTGAAGAAAT TAGACCAGGC TCAGTGGTCT CCAAGAAAGG ATATCTGCAT 
TTCAAGGAGC CCCTGTCCAG CAACTGGGCT AAACATTTCG TCGTGGTCCG TCGCCCTTAC 
GTCTTCATCT ATAACAGCGA CAAAGACCCT GTGGAGCGGG GCATCATTAA CCTGTCCACA 
GCCCAGGTGG AGTATAGCGA GGACCAGCAG GCCATGGTGA AGACACCGAA CACCTTTGCT 
GTGTGCACCA AGCACCGTGG GGTCCTTTTG CAGGCTCTCA ACGACAAAGA CGTGAACGAC 
TGGTTGTACG CCTTTAACCC GCTCCTTGCT GGCACAATAC GGTCAAAACT CTCCCGCAGA 
TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015466782.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322268.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92942
Clone ID Related Accession (Same CDS sequence)	XM_015466782.1 , XM_015475107.1
Accession Version	XM_015466782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGAC CATGAGAGAA AGATTGAACT GATCCGATTT 
CTCGTATCTG ACTATGTGAA TGTGCATGTG TTGGACACAT TTTCTGAGCA TGCCAACGTG 
CTTGCCTCCT CCGCCATTGC CACCTTCCAG GATGGGGCTG ACCCATCACC GCCTTTTCTC 
TTTCTGCCAG TTCCCAGCTG CCAGCCTGAG AGGGTACCTA AACGAGATGT TCCAGCCACC 
AAGTTAAATA CCATGAGCAA AACCAGCCTT GGCCAGAGCA TGAGCAAGTA TGATCTTCTG 
GTTTGGTTTG AGATCAGTGA ACTGGAGCCT ACGGGAGAGT ATATCCCAGC CGTGGTGGAC 
CATACAGCAG GCTTGCCCTG CCAGGGGACG TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG 
ATCACAGTGA CCATCATCCA TGAGAAGGGG AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG 
CTGGTGGTAG GCCGAATCAG GAATAAGGCT GAGGTGGATG AAGCTGCAGT GGACGCCATC 
CTCTCCCTCA ATATCATCTC TGCCAAGTAC CTGAAGTCGT CCCACAACTG TAGCAGGACC 
TTCTATCGTT TCGAGGCCGT CTGGGATAGT TCCCTCCATA ACTCCCTCCT TCTGAACCGA 
GTGACACCCT ACGGAGAAAA GATCTACATG ACCCTGTCGG CCTACCTGGA GCTGGATCAC 
TGCATCCAGC CGGCCGTCAT CACCAAGGAC GTGTGCATGG TCTTCTACTC CCGGGACGCC 
AAGATCTCGC CGCCACGCTC GCTGCGCAGC CTCTTTGGCA GCGGCTATTC GAAGTCACCA 
GACTCCAATC GAGTCACTGG AATTTATGAA CTCAGTTTAT GCAAAATGGC AGACACAGGA 
AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA ATCTTGGATA CCTCAGTGGC GTATGTGCGG 
GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC CGGGGAGACA GCCTCATCCT AGAGCACCAG 
TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC GAGGTGGAGA AAACCCGACA CTTTCTGCTG 
CTCCGTGACA GACTGGGTGA CAGCATCCCC AAATCCCTGA GTGACTCGTT GTCCCCCAGC 
CTCAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CTCAGATCTC AACCACCACC 
TTTGAAAGTG CCATCACACC CAGCGAGAGT AGTGGCTACG ACTCAGCAGA CATTGAAAGC 
CTGGTGGATC GAGAGAAAGA GCTGGCTACA AAGTGCCTGC AACTTCTCAC CCACACTTTC 
AACCGAGAAT TCAGCCAGGT GCACGGCAGC ATCAGTGACT GCAAGCTGGC TGACCTCTCC 
CCCGTCGGCC GGGACCCCTC TGTGTCCAGT CTGAGCAGTT CCACCCTCAC GCCATCCTCT 
ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC AACTCCCTGG ATCAGAAGAC CCCAGAAGCC 
AATTCCCGGG CCTCTAGTCC CTGCCCAGAA TTTGAACAGT TTCAGATTGT CCCACCTGTG 
GAAACACCCT ATTTGGCCCG AGCAGGGAAA AATGAATTTC TCAATCTTGT TCCAGATATT 
GAAGAAATTA GACCAGGCTC AGTGGTCTCC AAGAAAGGAT ATCTGCATTT CAAGGAGCCC 
CTGTCCAGCA ACTGGGCTAA ACATTTCGTC GTGGTCCGTC GCCCTTACGT CTTCATCTAT 
AACAGCGACA AAGACCCTGT GGAGCGGGGC ATCATTAACC TGTCCACAGC CCAGGTGGAG 
TATAGCGAGG ACCAGCAGGC CATGGTGAAG ACACCGAACA CCTTTGCTGT GTGCACCAAG 
CACCGTGGGG TCCTTTTGCA GGCTCTCAAC GACAAAGACG TGAACGACTG GTTGTACGCC 
TTTAACCCGC TCCTTGCTGG CACAATACGG TCAAAACTCT CCCGCAGATG CCCGAGCCAA 
GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322268.1
CDS	389..6040
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015466782.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466782.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGAC CATGAGAGAA AGATTGAACT GATCCGATTT 
CTCGTATCTG ACTATGTGAA TGTGCATGTG TTGGACACAT TTTCTGAGCA TGCCAACGTG 
CTTGCCTCCT CCGCCATTGC CACCTTCCAG GATGGGGCTG ACCCATCACC GCCTTTTCTC 
TTTCTGCCAG TTCCCAGCTG CCAGCCTGAG AGGGTACCTA AACGAGATGT TCCAGCCACC 
AAGTTAAATA CCATGAGCAA AACCAGCCTT GGCCAGAGCA TGAGCAAGTA TGATCTTCTG 
GTTTGGTTTG AGATCAGTGA ACTGGAGCCT ACGGGAGAGT ATATCCCAGC CGTGGTGGAC 
CATACAGCAG GCTTGCCCTG CCAGGGGACG TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG 
ATCACAGTGA CCATCATCCA TGAGAAGGGG AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG 
CTGGTGGTAG GCCGAATCAG GAATAAGGCT GAGGTGGATG AAGCTGCAGT GGACGCCATC 
CTCTCCCTCA ATATCATCTC TGCCAAGTAC CTGAAGTCGT CCCACAACTG TAGCAGGACC 
TTCTATCGTT TCGAGGCCGT CTGGGATAGT TCCCTCCATA ACTCCCTCCT TCTGAACCGA 
GTGACACCCT ACGGAGAAAA GATCTACATG ACCCTGTCGG CCTACCTGGA GCTGGATCAC 
TGCATCCAGC CGGCCGTCAT CACCAAGGAC GTGTGCATGG TCTTCTACTC CCGGGACGCC 
AAGATCTCGC CGCCACGCTC GCTGCGCAGC CTCTTTGGCA GCGGCTATTC GAAGTCACCA 
GACTCCAATC GAGTCACTGG AATTTATGAA CTCAGTTTAT GCAAAATGGC AGACACAGGA 
AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA ATCTTGGATA CCTCAGTGGC GTATGTGCGG 
GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC CGGGGAGACA GCCTCATCCT AGAGCACCAG 
TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC GAGGTGGAGA AAACCCGACA CTTTCTGCTG 
CTCCGTGACA GACTGGGTGA CAGCATCCCC AAATCCCTGA GTGACTCGTT GTCCCCCAGC 
CTCAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CTCAGATCTC AACCACCACC 
TTTGAAAGTG CCATCACACC CAGCGAGAGT AGTGGCTACG ACTCAGCAGA CATTGAAAGC 
CTGGTGGATC GAGAGAAAGA GCTGGCTACA AAGTGCCTGC AACTTCTCAC CCACACTTTC 
AACCGAGAAT TCAGCCAGGT GCACGGCAGC ATCAGTGACT GCAAGCTGGC TGACCTCTCC 
CCCGTCGGCC GGGACCCCTC TGTGTCCAGT CTGAGCAGTT CCACCCTCAC GCCATCCTCT 
ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC AACTCCCTGG ATCAGAAGAC CCCAGAAGCC 
AATTCCCGGG CCTCTAGTCC CTGCCCAGAA TTTGAACAGT TTCAGATTGT CCCACCTGTG 
GAAACACCCT ATTTGGCCCG AGCAGGGAAA AATGAATTTC TCAATCTTGT TCCAGATATT 
GAAGAAATTA GACCAGGCTC AGTGGTCTCC AAGAAAGGAT ATCTGCATTT CAAGGAGCCC 
CTGTCCAGCA ACTGGGCTAA ACATTTCGTC GTGGTCCGTC GCCCTTACGT CTTCATCTAT 
AACAGCGACA AAGACCCTGT GGAGCGGGGC ATCATTAACC TGTCCACAGC CCAGGTGGAG 
TATAGCGAGG ACCAGCAGGC CATGGTGAAG ACACCGAACA CCTTTGCTGT GTGCACCAAG 
CACCGTGGGG TCCTTTTGCA GGCTCTCAAC GACAAAGACG TGAACGACTG GTTGTACGCC 
TTTAACCCGC TCCTTGCTGG CACAATACGG TCAAAACTCT CCCGCAGATG CCCGAGCCAA 
GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015475107.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330593.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa92942
Clone ID Related Accession (Same CDS sequence)	XM_015466782.1 , XM_015475107.1
Accession Version	XM_015475107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGAC CATGAGAGAA AGATTGAACT GATCCGATTT 
CTCGTATCTG ACTATGTGAA TGTGCATGTG TTGGACACAT TTTCTGAGCA TGCCAACGTG 
CTTGCCTCCT CCGCCATTGC CACCTTCCAG GATGGGGCTG ACCCATCACC GCCTTTTCTC 
TTTCTGCCAG TTCCCAGCTG CCAGCCTGAG AGGGTACCTA AACGAGATGT TCCAGCCACC 
AAGTTAAATA CCATGAGCAA AACCAGCCTT GGCCAGAGCA TGAGCAAGTA TGATCTTCTG 
GTTTGGTTTG AGATCAGTGA ACTGGAGCCT ACGGGAGAGT ATATCCCAGC CGTGGTGGAC 
CATACAGCAG GCTTGCCCTG CCAGGGGACG TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG 
ATCACAGTGA CCATCATCCA TGAGAAGGGG AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG 
CTGGTGGTAG GCCGAATCAG GAATAAGGCT GAGGTGGATG AAGCTGCAGT GGACGCCATC 
CTCTCCCTCA ATATCATCTC TGCCAAGTAC CTGAAGTCGT CCCACAACTG TAGCAGGACC 
TTCTATCGTT TCGAGGCCGT CTGGGATAGT TCCCTCCATA ACTCCCTCCT TCTGAACCGA 
GTGACACCCT ACGGAGAAAA GATCTACATG ACCCTGTCGG CCTACCTGGA GCTGGATCAC 
TGCATCCAGC CGGCCGTCAT CACCAAGGAC GTGTGCATGG TCTTCTACTC CCGGGACGCC 
AAGATCTCGC CGCCACGCTC GCTGCGCAGC CTCTTTGGCA GCGGCTATTC GAAGTCACCA 
GACTCCAATC GAGTCACTGG AATTTATGAA CTCAGTTTAT GCAAAATGGC AGACACAGGA 
AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA ATCTTGGATA CCTCAGTGGC GTATGTGCGG 
GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC CGGGGAGACA GCCTCATCCT AGAGCACCAG 
TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC GAGGTGGAGA AAACCCGACA CTTTCTGCTG 
CTCCGTGACA GACTGGGTGA CAGCATCCCC AAATCCCTGA GTGACTCGTT GTCCCCCAGC 
CTCAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CTCAGATCTC AACCACCACC 
TTTGAAAGTG CCATCACACC CAGCGAGAGT AGTGGCTACG ACTCAGCAGA CATTGAAAGC 
CTGGTGGATC GAGAGAAAGA GCTGGCTACA AAGTGCCTGC AACTTCTCAC CCACACTTTC 
AACCGAGAAT TCAGCCAGGT GCACGGCAGC ATCAGTGACT GCAAGCTGGC TGACCTCTCC 
CCCGTCGGCC GGGACCCCTC TGTGTCCAGT CTGAGCAGTT CCACCCTCAC GCCATCCTCT 
ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC AACTCCCTGG ATCAGAAGAC CCCAGAAGCC 
AATTCCCGGG CCTCTAGTCC CTGCCCAGAA TTTGAACAGT TTCAGATTGT CCCACCTGTG 
GAAACACCCT ATTTGGCCCG AGCAGGGAAA AATGAATTTC TCAATCTTGT TCCAGATATT 
GAAGAAATTA GACCAGGCTC AGTGGTCTCC AAGAAAGGAT ATCTGCATTT CAAGGAGCCC 
CTGTCCAGCA ACTGGGCTAA ACATTTCGTC GTGGTCCGTC GCCCTTACGT CTTCATCTAT 
AACAGCGACA AAGACCCTGT GGAGCGGGGC ATCATTAACC TGTCCACAGC CCAGGTGGAG 
TATAGCGAGG ACCAGCAGGC CATGGTGAAG ACACCGAACA CCTTTGCTGT GTGCACCAAG 
CACCGTGGGG TCCTTTTGCA GGCTCTCAAC GACAAAGACG TGAACGACTG GTTGTACGCC 
TTTAACCCGC TCCTTGCTGG CACAATACGG TCAAAACTCT CCCGCAGATG CCCGAGCCAA 
GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330593.1
CDS	389..6040
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015475107.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475107.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGAC CATGAGAGAA AGATTGAACT GATCCGATTT 
CTCGTATCTG ACTATGTGAA TGTGCATGTG TTGGACACAT TTTCTGAGCA TGCCAACGTG 
CTTGCCTCCT CCGCCATTGC CACCTTCCAG GATGGGGCTG ACCCATCACC GCCTTTTCTC 
TTTCTGCCAG TTCCCAGCTG CCAGCCTGAG AGGGTACCTA AACGAGATGT TCCAGCCACC 
AAGTTAAATA CCATGAGCAA AACCAGCCTT GGCCAGAGCA TGAGCAAGTA TGATCTTCTG 
GTTTGGTTTG AGATCAGTGA ACTGGAGCCT ACGGGAGAGT ATATCCCAGC CGTGGTGGAC 
CATACAGCAG GCTTGCCCTG CCAGGGGACG TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG 
ATCACAGTGA CCATCATCCA TGAGAAGGGG AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG 
CTGGTGGTAG GCCGAATCAG GAATAAGGCT GAGGTGGATG AAGCTGCAGT GGACGCCATC 
CTCTCCCTCA ATATCATCTC TGCCAAGTAC CTGAAGTCGT CCCACAACTG TAGCAGGACC 
TTCTATCGTT TCGAGGCCGT CTGGGATAGT TCCCTCCATA ACTCCCTCCT TCTGAACCGA 
GTGACACCCT ACGGAGAAAA GATCTACATG ACCCTGTCGG CCTACCTGGA GCTGGATCAC 
TGCATCCAGC CGGCCGTCAT CACCAAGGAC GTGTGCATGG TCTTCTACTC CCGGGACGCC 
AAGATCTCGC CGCCACGCTC GCTGCGCAGC CTCTTTGGCA GCGGCTATTC GAAGTCACCA 
GACTCCAATC GAGTCACTGG AATTTATGAA CTCAGTTTAT GCAAAATGGC AGACACAGGA 
AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA ATCTTGGATA CCTCAGTGGC GTATGTGCGG 
GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC CGGGGAGACA GCCTCATCCT AGAGCACCAG 
TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC GAGGTGGAGA AAACCCGACA CTTTCTGCTG 
CTCCGTGACA GACTGGGTGA CAGCATCCCC AAATCCCTGA GTGACTCGTT GTCCCCCAGC 
CTCAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CTCAGATCTC AACCACCACC 
TTTGAAAGTG CCATCACACC CAGCGAGAGT AGTGGCTACG ACTCAGCAGA CATTGAAAGC 
CTGGTGGATC GAGAGAAAGA GCTGGCTACA AAGTGCCTGC AACTTCTCAC CCACACTTTC 
AACCGAGAAT TCAGCCAGGT GCACGGCAGC ATCAGTGACT GCAAGCTGGC TGACCTCTCC 
CCCGTCGGCC GGGACCCCTC TGTGTCCAGT CTGAGCAGTT CCACCCTCAC GCCATCCTCT 
ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC AACTCCCTGG ATCAGAAGAC CCCAGAAGCC 
AATTCCCGGG CCTCTAGTCC CTGCCCAGAA TTTGAACAGT TTCAGATTGT CCCACCTGTG 
GAAACACCCT ATTTGGCCCG AGCAGGGAAA AATGAATTTC TCAATCTTGT TCCAGATATT 
GAAGAAATTA GACCAGGCTC AGTGGTCTCC AAGAAAGGAT ATCTGCATTT CAAGGAGCCC 
CTGTCCAGCA ACTGGGCTAA ACATTTCGTC GTGGTCCGTC GCCCTTACGT CTTCATCTAT 
AACAGCGACA AAGACCCTGT GGAGCGGGGC ATCATTAACC TGTCCACAGC CCAGGTGGAG 
TATAGCGAGG ACCAGCAGGC CATGGTGAAG ACACCGAACA CCTTTGCTGT GTGCACCAAG 
CACCGTGGGG TCCTTTTGCA GGCTCTCAAC GACAAAGACG TGAACGACTG GTTGTACGCC 
TTTAACCCGC TCCTTGCTGG CACAATACGG TCAAAACTCT CCCGCAGATG CCCGAGCCAA 
GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015466783.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322269.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.

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CloneID	OBa92943
Clone ID Related Accession (Same CDS sequence)	XM_015466783.1 , XM_015475108.1
Accession Version	XM_015466783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TATTGATCCA TTGATCGATG ATTACTCGGG AAGTGGAGGC 
AAATATCTGA AAGATTTTCA GAACAATAAG CATAGGTACT TGCTAGCCTC TGAGAATCAA 
CGTCCTGGCA ATTTTTCCAC AGCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322269.1
CDS	388..6021
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015466783.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466783.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TATTGATCCA TTGATCGATG ATTACTCGGG AAGTGGAGGC 
AAATATCTGA AAGATTTTCA GAACAATAAG CATAGGTACT TGCTAGCCTC TGAGAATCAA 
CGTCCTGGCA ATTTTTCCAC AGCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015475108.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330594.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.

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CloneID	OBa92943
Clone ID Related Accession (Same CDS sequence)	XM_015466783.1 , XM_015475108.1
Accession Version	XM_015475108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TATTGATCCA TTGATCGATG ATTACTCGGG AAGTGGAGGC 
AAATATCTGA AAGATTTTCA GAACAATAAG CATAGGTACT TGCTAGCCTC TGAGAATCAA 
CGTCCTGGCA ATTTTTCCAC AGCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330594.1
CDS	388..6021
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015475108.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475108.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TATTGATCCA TTGATCGATG ATTACTCGGG AAGTGGAGGC 
AAATATCTGA AAGATTTTCA GAACAATAAG CATAGGTACT TGCTAGCCTC TGAGAATCAA 
CGTCCTGGCA ATTTTTCCAC AGCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015475109.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330595.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.

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CloneID	OBa92944
Clone ID Related Accession (Same CDS sequence)	XM_015475109.1 , XM_015466784.1
Accession Version	XM_015475109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330595.1
CDS	388..5961
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKSSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015475109.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475109.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015466784.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322270.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.

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CloneID	OBa92944
Clone ID Related Accession (Same CDS sequence)	XM_015475109.1 , XM_015466784.1
Accession Version	XM_015466784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322270.1
CDS	388..5961
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKSSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015466784.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466784.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015475110.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330596.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.

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CloneID	OBa92945
Clone ID Related Accession (Same CDS sequence)	XM_015475110.1 , XM_015466785.1
Accession Version	XM_015475110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGAC CATGAGAGAA AGATTGAACT GATCCGATTT 
CTCGTATCTG ACTATGTGAA TGTGCATGTG TTGGACACAT TTTCTGAGCA TGCCAACGTG 
CTTGCCTCCT CCGCCATTGC CACCTTCCAG GATGGGGCTG ACCCATCACC GCCTTTTCTC 
TTTCTGCCAG TTCCCAGCTG CCAGCCTGAG AGGGTACCTA AACGAGATGT TCCAGCCACC 
AAGTTAAATA CCATGAGCAA AACCAGCCTT GGCCAGAGCA TGAGCAAGTA TGATCTTCTG 
GTTTGGTTTG AGATCAGTGA ACTGGAGCCT ACGGGAGAGT ATATCCCAGC CGTGGTGGAC 
CATACAGCAG GCTTGCCCTG CCAGGGGACG TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG 
ATCACAGTGA CCATCATCCA TGAGAAGGGG AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG 
CTGGTGGTAG GCCGAATCAG GAATAAGGCT GAGGTGGATG AAGCTGCAGT GGACGCCATC 
CTCTCCCTCA ATATCATCTC TGCCAAGTAC CTGAAGTCGT CCCACAACTG TAGCAGGACC 
TTCTATCGTT TCGAGGCCGT CTGGGATAGT TCCCTCCATA ACTCCCTCCT TCTGAACCGA 
GTGACACCCT ACGGAGAAAA GATCTACATG ACCCTGTCGG CCTACCTGGA GCTGGATCAC 
TGCATCCAGC CGGCCGTCAT CACCAAGGAC GTGTGCATGG TCTTCTACTC CCGGGACGCC 
AAGATCTCGC CGCCACGCTC GCTGCGCAGC CTCTTTGGCA GCGGCTATTC GAAGTCACCA 
GACTCCAATC GAGTCACTGG AATTTATGAA CTCAGTTTAT GCAAAATGGC AGACACAGGA 
AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA ATCTTGGATA CCTCAGTGGC GTATGTGCGG 
GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC CGGGGAGACA GCCTCATCCT AGAGCACCAG 
TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC GAGGTGGAGA AAACCCGACA CTTTCTGCTG 
CTCCGTGACA GACTGGGTGA CAGCATCCCC AAATCCCTGA GTGACTCGTT GTCCCCCAGC 
CTCAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CTCAGATCTC AACCACCACC 
TTTGAAAGTG CCATCACACC CAGCGAGAGT AGTGGCTACG ACTCAGCAGA CATTGAAAGC 
CTGGTGGATC GAGAGAAAGA GCTGGCTACA AAGTGCCTGC AACTTCTCAC CCACACTTTC 
AACCGAGAAT TCAGCCAGGT GCACGGCAGC ATCAGTGACT GCAAGCTGGC TGACCTCTCC 
CCCGTCGGCC GGGACCCCTC TGTGTCCAGT CTGAGCAGTT CCACCCTCAC GCCATCCTCT 
ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC AACTCCCTGG ATCAGAAGAC CCCAGAAGCC 
AATTCCCGGG CCTCTAGTCC CTGCCCAGAA TTTGAACAGT TTCAGATTGT CCCACCTGTG 
GAAACACCCT ATTTGGCCCG AGCAGGGAAA AATGAATTTC TCAATCTTGT TCCAGATATT 
GAAGAAATTA GACCAGGCTC AGTGGTCTCC AAGAAAGGAT ATCTGCATTT CAAGGAGCCC 
CTGTCCAGCA ACTGGGCTAA ACATTTCGTC GTGGTCCGTC GCCCTTACGT CTTCATCTAT 
AACAGCGACA AAGACCCTGT GGAGCGGGGC ATCATTAACC TGTCCACAGC CCAGGTGGAG 
TATAGCGAGG ACCAGCAGGC CATGGTGAAG ACACCGAACA CCTTTGCTGT GTGCACCAAG 
CACCGTGGGG TCCTTTTGCA GGCTCTCAAC GACAAAGACG TGAACGACTG GTTGTACGCC 
TTTAACCCGC TCCTTGCTGG CACAATACGG TCAAAACTCT CCCGCAGATG CCCGAGCCAA 
GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330596.1
CDS	388..5919
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015475110.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475110.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGAC CATGAGAGAA AGATTGAACT GATCCGATTT 
CTCGTATCTG ACTATGTGAA TGTGCATGTG TTGGACACAT TTTCTGAGCA TGCCAACGTG 
CTTGCCTCCT CCGCCATTGC CACCTTCCAG GATGGGGCTG ACCCATCACC GCCTTTTCTC 
TTTCTGCCAG TTCCCAGCTG CCAGCCTGAG AGGGTACCTA AACGAGATGT TCCAGCCACC 
AAGTTAAATA CCATGAGCAA AACCAGCCTT GGCCAGAGCA TGAGCAAGTA TGATCTTCTG 
GTTTGGTTTG AGATCAGTGA ACTGGAGCCT ACGGGAGAGT ATATCCCAGC CGTGGTGGAC 
CATACAGCAG GCTTGCCCTG CCAGGGGACG TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG 
ATCACAGTGA CCATCATCCA TGAGAAGGGG AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG 
CTGGTGGTAG GCCGAATCAG GAATAAGGCT GAGGTGGATG AAGCTGCAGT GGACGCCATC 
CTCTCCCTCA ATATCATCTC TGCCAAGTAC CTGAAGTCGT CCCACAACTG TAGCAGGACC 
TTCTATCGTT TCGAGGCCGT CTGGGATAGT TCCCTCCATA ACTCCCTCCT TCTGAACCGA 
GTGACACCCT ACGGAGAAAA GATCTACATG ACCCTGTCGG CCTACCTGGA GCTGGATCAC 
TGCATCCAGC CGGCCGTCAT CACCAAGGAC GTGTGCATGG TCTTCTACTC CCGGGACGCC 
AAGATCTCGC CGCCACGCTC GCTGCGCAGC CTCTTTGGCA GCGGCTATTC GAAGTCACCA 
GACTCCAATC GAGTCACTGG AATTTATGAA CTCAGTTTAT GCAAAATGGC AGACACAGGA 
AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA ATCTTGGATA CCTCAGTGGC GTATGTGCGG 
GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC CGGGGAGACA GCCTCATCCT AGAGCACCAG 
TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC GAGGTGGAGA AAACCCGACA CTTTCTGCTG 
CTCCGTGACA GACTGGGTGA CAGCATCCCC AAATCCCTGA GTGACTCGTT GTCCCCCAGC 
CTCAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CTCAGATCTC AACCACCACC 
TTTGAAAGTG CCATCACACC CAGCGAGAGT AGTGGCTACG ACTCAGCAGA CATTGAAAGC 
CTGGTGGATC GAGAGAAAGA GCTGGCTACA AAGTGCCTGC AACTTCTCAC CCACACTTTC 
AACCGAGAAT TCAGCCAGGT GCACGGCAGC ATCAGTGACT GCAAGCTGGC TGACCTCTCC 
CCCGTCGGCC GGGACCCCTC TGTGTCCAGT CTGAGCAGTT CCACCCTCAC GCCATCCTCT 
ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC AACTCCCTGG ATCAGAAGAC CCCAGAAGCC 
AATTCCCGGG CCTCTAGTCC CTGCCCAGAA TTTGAACAGT TTCAGATTGT CCCACCTGTG 
GAAACACCCT ATTTGGCCCG AGCAGGGAAA AATGAATTTC TCAATCTTGT TCCAGATATT 
GAAGAAATTA GACCAGGCTC AGTGGTCTCC AAGAAAGGAT ATCTGCATTT CAAGGAGCCC 
CTGTCCAGCA ACTGGGCTAA ACATTTCGTC GTGGTCCGTC GCCCTTACGT CTTCATCTAT 
AACAGCGACA AAGACCCTGT GGAGCGGGGC ATCATTAACC TGTCCACAGC CCAGGTGGAG 
TATAGCGAGG ACCAGCAGGC CATGGTGAAG ACACCGAACA CCTTTGCTGT GTGCACCAAG 
CACCGTGGGG TCCTTTTGCA GGCTCTCAAC GACAAAGACG TGAACGACTG GTTGTACGCC 
TTTAACCCGC TCCTTGCTGG CACAATACGG TCAAAACTCT CCCGCAGATG CCCGAGCCAA 
GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015466785.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322271.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.

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CloneID	OBa92945
Clone ID Related Accession (Same CDS sequence)	XM_015475110.1 , XM_015466785.1
Accession Version	XM_015466785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGAC CATGAGAGAA AGATTGAACT GATCCGATTT 
CTCGTATCTG ACTATGTGAA TGTGCATGTG TTGGACACAT TTTCTGAGCA TGCCAACGTG 
CTTGCCTCCT CCGCCATTGC CACCTTCCAG GATGGGGCTG ACCCATCACC GCCTTTTCTC 
TTTCTGCCAG TTCCCAGCTG CCAGCCTGAG AGGGTACCTA AACGAGATGT TCCAGCCACC 
AAGTTAAATA CCATGAGCAA AACCAGCCTT GGCCAGAGCA TGAGCAAGTA TGATCTTCTG 
GTTTGGTTTG AGATCAGTGA ACTGGAGCCT ACGGGAGAGT ATATCCCAGC CGTGGTGGAC 
CATACAGCAG GCTTGCCCTG CCAGGGGACG TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG 
ATCACAGTGA CCATCATCCA TGAGAAGGGG AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG 
CTGGTGGTAG GCCGAATCAG GAATAAGGCT GAGGTGGATG AAGCTGCAGT GGACGCCATC 
CTCTCCCTCA ATATCATCTC TGCCAAGTAC CTGAAGTCGT CCCACAACTG TAGCAGGACC 
TTCTATCGTT TCGAGGCCGT CTGGGATAGT TCCCTCCATA ACTCCCTCCT TCTGAACCGA 
GTGACACCCT ACGGAGAAAA GATCTACATG ACCCTGTCGG CCTACCTGGA GCTGGATCAC 
TGCATCCAGC CGGCCGTCAT CACCAAGGAC GTGTGCATGG TCTTCTACTC CCGGGACGCC 
AAGATCTCGC CGCCACGCTC GCTGCGCAGC CTCTTTGGCA GCGGCTATTC GAAGTCACCA 
GACTCCAATC GAGTCACTGG AATTTATGAA CTCAGTTTAT GCAAAATGGC AGACACAGGA 
AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA ATCTTGGATA CCTCAGTGGC GTATGTGCGG 
GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC CGGGGAGACA GCCTCATCCT AGAGCACCAG 
TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC GAGGTGGAGA AAACCCGACA CTTTCTGCTG 
CTCCGTGACA GACTGGGTGA CAGCATCCCC AAATCCCTGA GTGACTCGTT GTCCCCCAGC 
CTCAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CTCAGATCTC AACCACCACC 
TTTGAAAGTG CCATCACACC CAGCGAGAGT AGTGGCTACG ACTCAGCAGA CATTGAAAGC 
CTGGTGGATC GAGAGAAAGA GCTGGCTACA AAGTGCCTGC AACTTCTCAC CCACACTTTC 
AACCGAGAAT TCAGCCAGGT GCACGGCAGC ATCAGTGACT GCAAGCTGGC TGACCTCTCC 
CCCGTCGGCC GGGACCCCTC TGTGTCCAGT CTGAGCAGTT CCACCCTCAC GCCATCCTCT 
ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC AACTCCCTGG ATCAGAAGAC CCCAGAAGCC 
AATTCCCGGG CCTCTAGTCC CTGCCCAGAA TTTGAACAGT TTCAGATTGT CCCACCTGTG 
GAAACACCCT ATTTGGCCCG AGCAGGGAAA AATGAATTTC TCAATCTTGT TCCAGATATT 
GAAGAAATTA GACCAGGCTC AGTGGTCTCC AAGAAAGGAT ATCTGCATTT CAAGGAGCCC 
CTGTCCAGCA ACTGGGCTAA ACATTTCGTC GTGGTCCGTC GCCCTTACGT CTTCATCTAT 
AACAGCGACA AAGACCCTGT GGAGCGGGGC ATCATTAACC TGTCCACAGC CCAGGTGGAG 
TATAGCGAGG ACCAGCAGGC CATGGTGAAG ACACCGAACA CCTTTGCTGT GTGCACCAAG 
CACCGTGGGG TCCTTTTGCA GGCTCTCAAC GACAAAGACG TGAACGACTG GTTGTACGCC 
TTTAACCCGC TCCTTGCTGG CACAATACGG TCAAAACTCT CCCGCAGATG CCCGAGCCAA 
GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322271.1
CDS	388..5919
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015466785.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466785.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGAC CATGAGAGAA AGATTGAACT GATCCGATTT 
CTCGTATCTG ACTATGTGAA TGTGCATGTG TTGGACACAT TTTCTGAGCA TGCCAACGTG 
CTTGCCTCCT CCGCCATTGC CACCTTCCAG GATGGGGCTG ACCCATCACC GCCTTTTCTC 
TTTCTGCCAG TTCCCAGCTG CCAGCCTGAG AGGGTACCTA AACGAGATGT TCCAGCCACC 
AAGTTAAATA CCATGAGCAA AACCAGCCTT GGCCAGAGCA TGAGCAAGTA TGATCTTCTG 
GTTTGGTTTG AGATCAGTGA ACTGGAGCCT ACGGGAGAGT ATATCCCAGC CGTGGTGGAC 
CATACAGCAG GCTTGCCCTG CCAGGGGACG TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG 
ATCACAGTGA CCATCATCCA TGAGAAGGGG AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG 
CTGGTGGTAG GCCGAATCAG GAATAAGGCT GAGGTGGATG AAGCTGCAGT GGACGCCATC 
CTCTCCCTCA ATATCATCTC TGCCAAGTAC CTGAAGTCGT CCCACAACTG TAGCAGGACC 
TTCTATCGTT TCGAGGCCGT CTGGGATAGT TCCCTCCATA ACTCCCTCCT TCTGAACCGA 
GTGACACCCT ACGGAGAAAA GATCTACATG ACCCTGTCGG CCTACCTGGA GCTGGATCAC 
TGCATCCAGC CGGCCGTCAT CACCAAGGAC GTGTGCATGG TCTTCTACTC CCGGGACGCC 
AAGATCTCGC CGCCACGCTC GCTGCGCAGC CTCTTTGGCA GCGGCTATTC GAAGTCACCA 
GACTCCAATC GAGTCACTGG AATTTATGAA CTCAGTTTAT GCAAAATGGC AGACACAGGA 
AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA ATCTTGGATA CCTCAGTGGC GTATGTGCGG 
GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC CGGGGAGACA GCCTCATCCT AGAGCACCAG 
TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC GAGGTGGAGA AAACCCGACA CTTTCTGCTG 
CTCCGTGACA GACTGGGTGA CAGCATCCCC AAATCCCTGA GTGACTCGTT GTCCCCCAGC 
CTCAGCAGCG GGACCCTCAG CACCTCCACC AGCATCTCCT CTCAGATCTC AACCACCACC 
TTTGAAAGTG CCATCACACC CAGCGAGAGT AGTGGCTACG ACTCAGCAGA CATTGAAAGC 
CTGGTGGATC GAGAGAAAGA GCTGGCTACA AAGTGCCTGC AACTTCTCAC CCACACTTTC 
AACCGAGAAT TCAGCCAGGT GCACGGCAGC ATCAGTGACT GCAAGCTGGC TGACCTCTCC 
CCCGTCGGCC GGGACCCCTC TGTGTCCAGT CTGAGCAGTT CCACCCTCAC GCCATCCTCT 
ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC AACTCCCTGG ATCAGAAGAC CCCAGAAGCC 
AATTCCCGGG CCTCTAGTCC CTGCCCAGAA TTTGAACAGT TTCAGATTGT CCCACCTGTG 
GAAACACCCT ATTTGGCCCG AGCAGGGAAA AATGAATTTC TCAATCTTGT TCCAGATATT 
GAAGAAATTA GACCAGGCTC AGTGGTCTCC AAGAAAGGAT ATCTGCATTT CAAGGAGCCC 
CTGTCCAGCA ACTGGGCTAA ACATTTCGTC GTGGTCCGTC GCCCTTACGT CTTCATCTAT 
AACAGCGACA AAGACCCTGT GGAGCGGGGC ATCATTAACC TGTCCACAGC CCAGGTGGAG 
TATAGCGAGG ACCAGCAGGC CATGGTGAAG ACACCGAACA CCTTTGCTGT GTGCACCAAG 
CACCGTGGGG TCCTTTTGCA GGCTCTCAAC GACAAAGACG TGAACGACTG GTTGTACGCC 
TTTAACCCGC TCCTTGCTGG CACAATACGG TCAAAACTCT CCCGCAGATG CCCGAGCCAA 
GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015466786.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322272.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.

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CloneID	OBa92946
Clone ID Related Accession (Same CDS sequence)	XM_015466786.1 , XM_015475111.1
Accession Version	XM_015466786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322272.1
CDS	388..5901
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015466786.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466786.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015475111.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330597.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.

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CloneID	OBa92946
Clone ID Related Accession (Same CDS sequence)	XM_015466786.1 , XM_015475111.1
Accession Version	XM_015475111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330597.1
CDS	388..5901
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015475111.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475111.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG ACCATGAGAG AAAGATTGAA CTGATCCGAT TTCTCGTATC TGACTATGTG 
AATGTGCATG TGTTGGACAC ATTTTCTGAG CATGCCAACG TGCTTGCCTC CTCCGCCATT 
GCCACCTTCC AGGATGGGGC TGACCCATCA CCGCCTTTTC TCTTTCTGCC AGTTCCCAGC 
TGCCAGCCTG AGAGGGTACC TAAACGAGAT GTTCCAGCCA CCAAGTTAAA TACCATGAGC 
AAAACCAGCC TTGGCCAGAG CATGAGCAAG TATGATCTTC TGGTTTGGTT TGAGATCAGT 
GAACTGGAGC CTACGGGAGA GTATATCCCA GCCGTGGTGG ACCATACAGC AGGCTTGCCC 
TGCCAGGGGA CGTTTTTGCT TCACCAGGGC ATCCAGCGGA GGATCACAGT GACCATCATC 
CATGAGAAGG GGAGTGAGCT CCACTGGAAG GATGTTCGTG AGCTGGTGGT AGGCCGAATC 
AGGAATAAGG CTGAGGTGGA TGAAGCTGCA GTGGACGCCA TCCTCTCCCT CAATATCATC 
TCTGCCAAGT ACCTGAAGTC GTCCCACAAC TGTAGCAGGA CCTTCTATCG TTTCGAGGCC 
GTCTGGGATA GTTCCCTCCA TAACTCCCTC CTTCTGAACC GAGTGACACC CTACGGAGAA 
AAGATCTACA TGACCCTGTC GGCCTACCTG GAGCTGGATC ACTGCATCCA GCCGGCCGTC 
ATCACCAAGG ACGTGTGCAT GGTCTTCTAC TCCCGGGACG CCAAGATCTC GCCGCCACGC 
TCGCTGCGCA GCCTCTTTGG CAGCGGCTAT TCGAAGTCAC CAGACTCCAA TCGAGTCACT 
GGAATTTATG AACTCAGTTT ATGCAAAATG GCAGACACAG GAAGTCCAGG CATGCAGCGG 
AGAAGAAGGA AAATCTTGGA TACCTCAGTG GCGTATGTGC GGGGGGAGGA GAACTTGGCT 
GGCTGGCGGC CCCGGGGAGA CAGCCTCATC CTAGAGCACC AGTGGGAGTT GGAGAAGCTG 
GAGCTCCTGC ACGAGGTGGA GAAAACCCGA CACTTTCTGC TGCTCCGTGA CAGACTGGGT 
GACAGCATCC CCAAATCCCT GAGTGACTCG TTGTCCCCCA GCCTCAGCAG CGGGACCCTC 
AGCACCTCCA CCAGCATCTC CTCTCAGATC TCAACCACCA CCTTTGAAAG TGCCATCACA 
CCCAGCGAGA GTAGTGGCTA CGACTCAGCA GACATTGAAA GCCTGGTGGA TCGAGAGAAA 
GAGCTGGCTA CAAAGTGCCT GCAACTTCTC ACCCACACTT TCAACCGAGA ATTCAGCCAG 
GTGCACGGCA GCATCAGTGA CTGCAAGCTG GCTGACCTCT CCCCCGTCGG CCGGGACCCC 
TCTGTGTCCA GTCTGAGCAG TTCCACCCTC ACGCCATCCT CTACCTGCCC CTCTCTGGTG 
GATGCTAGGA GCAACTCCCT GGATCAGAAG ACCCCAGAAG CCAATTCCCG GGCCTCTAGT 
CCCTGCCCAG AATTTGAACA GTTTCAGATT GTCCCACCTG TGGAAACACC CTATTTGGCC 
CGAGCAGGGA AAAATGAATT TCTCAATCTT GTTCCAGATA TTGAAGAAAT TAGACCAGGC 
TCAGTGGTCT CCAAGAAAGG ATATCTGCAT TTCAAGGAGC CCCTGTCCAG CAACTGGGCT 
AAACATTTCG TCGTGGTCCG TCGCCCTTAC GTCTTCATCT ATAACAGCGA CAAAGACCCT 
GTGGAGCGGG GCATCATTAA CCTGTCCACA GCCCAGGTGG AGTATAGCGA GGACCAGCAG 
GCCATGGTGA AGACACCGAA CACCTTTGCT GTGTGCACCA AGCACCGTGG GGTCCTTTTG 
CAGGCTCTCA ACGACAAAGA CGTGAACGAC TGGTTGTACG CCTTTAACCC GCTCCTTGCT 
GGCACAATAC GGTCAAAACT CTCCCGCAGA TGCCCGAGCC AAGCGAAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015475112.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330598.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.

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CloneID	OBa92947
Clone ID Related Accession (Same CDS sequence)	XM_015475112.1 , XM_015466787.1
Accession Version	XM_015475112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGA CCATGAGAGA AAGATTGAAC TGATCCGATT TCTCGTATCT 
GACTATGTGA ATGTGCATGT GTTGGACACA TTTTCTGAGC ATGCCAACGT GCTTGCCTCC 
TCCGCCATTG CCACCTTCCA GGATGGGGCT GACCCATCAC CGCCTTTTCT CTTTCTGCCA 
GTTCCCAGCT GCCAGCCTGA GAGGGTACCT AAACGAGATG TTCCAGCCAC CAAGTTAAAT 
ACCATGAGCA AAACCAGCCT TGGCCAGAGC ATGAGCAAGT ATGATCTTCT GGTTTGGTTT 
GAGATCAGTG AACTGGAGCC TACGGGAGAG TATATCCCAG CCGTGGTGGA CCATACAGCA 
GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT CACCAGGGCA TCCAGCGGAG GATCACAGTG 
ACCATCATCC ATGAGAAGGG GAGTGAGCTC CACTGGAAGG ATGTTCGTGA GCTGGTGGTA 
GGCCGAATCA GGAATAAGGC TGAGGTGGAT GAAGCTGCAG TGGACGCCAT CCTCTCCCTC 
AATATCATCT CTGCCAAGTA CCTGAAGTCG TCCCACAACT GTAGCAGGAC CTTCTATCGT 
TTCGAGGCCG TCTGGGATAG TTCCCTCCAT AACTCCCTCC TTCTGAACCG AGTGACACCC 
TACGGAGAAA AGATCTACAT GACCCTGTCG GCCTACCTGG AGCTGGATCA CTGCATCCAG 
CCGGCCGTCA TCACCAAGGA CGTGTGCATG GTCTTCTACT CCCGGGACGC CAAGATCTCG 
CCGCCACGCT CGCTGCGCAG CCTCTTTGGC AGCGGCTATT CGAAGTCACC AGACTCCAAT 
CGAGTCACTG GAATTTATGA ACTCAGTTTA TGCAAAATGG CAGACACAGG AAGTCCAGGC 
ATGCAGCGGA GAAGAAGGAA AATCTTGGAT ACCTCAGTGG CGTATGTGCG GGGGGAGGAG 
AACTTGGCTG GCTGGCGGCC CCGGGGAGAC AGCCTCATCC TAGAGCACCA GTGGGAGTTG 
GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG AAAACCCGAC ACTTTCTGCT GCTCCGTGAC 
AGACTGGGTG ACAGCATCCC CAAATCCCTG AGTGACTCGT TGTCCCCCAG CCTCAGCAGC 
GGGACCCTCA GCACCTCCAC CAGCATCTCC TCTCAGATCT CAACCACCAC CTTTGAAAGT 
GCCATCACAC CCAGCGAGAG TAGTGGCTAC GACTCAGCAG ACATTGAAAG CCTGGTGGAT 
CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG CAACTTCTCA CCCACACTTT CAACCGAGAA 
TTCAGCCAGG TGCACGGCAG CATCAGTGAC TGCAAGCTGG CTGACCTCTC CCCCGTCGGC 
CGGGACCCCT CTGTGTCCAG TCTGAGCAGT TCCACCCTCA CGCCATCCTC TACCTGCCCC 
TCTCTGGTGG ATGCTAGGAG CAACTCCCTG GATCAGAAGA CCCCAGAAGC CAATTCCCGG 
GCCTCTAGTC CCTGCCCAGA ATTTGAACAG TTTCAGATTG TCCCACCTGT GGAAACACCC 
TATTTGGCCC GAGCAGGGAA AAATGAATTT CTCAATCTTG TTCCAGATAT TGAAGAAATT 
AGACCAGGCT CAGTGGTCTC CAAGAAAGGA TATCTGCATT TCAAGGAGCC CCTGTCCAGC 
AACTGGGCTA AACATTTCGT CGTGGTCCGT CGCCCTTACG TCTTCATCTA TAACAGCGAC 
AAAGACCCTG TGGAGCGGGG CATCATTAAC CTGTCCACAG CCCAGGTGGA GTATAGCGAG 
GACCAGCAGG CCATGGTGAA GACACCGAAC ACCTTTGCTG TGTGCACCAA GCACCGTGGG 
GTCCTTTTGC AGGCTCTCAA CGACAAAGAC GTGAACGACT GGTTGTACGC CTTTAACCCG 
CTCCTTGCTG GCACAATACG GTCAAAACTC TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330598.1
CDS	388..5790
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015475112.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475112.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGA CCATGAGAGA AAGATTGAAC TGATCCGATT TCTCGTATCT 
GACTATGTGA ATGTGCATGT GTTGGACACA TTTTCTGAGC ATGCCAACGT GCTTGCCTCC 
TCCGCCATTG CCACCTTCCA GGATGGGGCT GACCCATCAC CGCCTTTTCT CTTTCTGCCA 
GTTCCCAGCT GCCAGCCTGA GAGGGTACCT AAACGAGATG TTCCAGCCAC CAAGTTAAAT 
ACCATGAGCA AAACCAGCCT TGGCCAGAGC ATGAGCAAGT ATGATCTTCT GGTTTGGTTT 
GAGATCAGTG AACTGGAGCC TACGGGAGAG TATATCCCAG CCGTGGTGGA CCATACAGCA 
GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT CACCAGGGCA TCCAGCGGAG GATCACAGTG 
ACCATCATCC ATGAGAAGGG GAGTGAGCTC CACTGGAAGG ATGTTCGTGA GCTGGTGGTA 
GGCCGAATCA GGAATAAGGC TGAGGTGGAT GAAGCTGCAG TGGACGCCAT CCTCTCCCTC 
AATATCATCT CTGCCAAGTA CCTGAAGTCG TCCCACAACT GTAGCAGGAC CTTCTATCGT 
TTCGAGGCCG TCTGGGATAG TTCCCTCCAT AACTCCCTCC TTCTGAACCG AGTGACACCC 
TACGGAGAAA AGATCTACAT GACCCTGTCG GCCTACCTGG AGCTGGATCA CTGCATCCAG 
CCGGCCGTCA TCACCAAGGA CGTGTGCATG GTCTTCTACT CCCGGGACGC CAAGATCTCG 
CCGCCACGCT CGCTGCGCAG CCTCTTTGGC AGCGGCTATT CGAAGTCACC AGACTCCAAT 
CGAGTCACTG GAATTTATGA ACTCAGTTTA TGCAAAATGG CAGACACAGG AAGTCCAGGC 
ATGCAGCGGA GAAGAAGGAA AATCTTGGAT ACCTCAGTGG CGTATGTGCG GGGGGAGGAG 
AACTTGGCTG GCTGGCGGCC CCGGGGAGAC AGCCTCATCC TAGAGCACCA GTGGGAGTTG 
GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG AAAACCCGAC ACTTTCTGCT GCTCCGTGAC 
AGACTGGGTG ACAGCATCCC CAAATCCCTG AGTGACTCGT TGTCCCCCAG CCTCAGCAGC 
GGGACCCTCA GCACCTCCAC CAGCATCTCC TCTCAGATCT CAACCACCAC CTTTGAAAGT 
GCCATCACAC CCAGCGAGAG TAGTGGCTAC GACTCAGCAG ACATTGAAAG CCTGGTGGAT 
CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG CAACTTCTCA CCCACACTTT CAACCGAGAA 
TTCAGCCAGG TGCACGGCAG CATCAGTGAC TGCAAGCTGG CTGACCTCTC CCCCGTCGGC 
CGGGACCCCT CTGTGTCCAG TCTGAGCAGT TCCACCCTCA CGCCATCCTC TACCTGCCCC 
TCTCTGGTGG ATGCTAGGAG CAACTCCCTG GATCAGAAGA CCCCAGAAGC CAATTCCCGG 
GCCTCTAGTC CCTGCCCAGA ATTTGAACAG TTTCAGATTG TCCCACCTGT GGAAACACCC 
TATTTGGCCC GAGCAGGGAA AAATGAATTT CTCAATCTTG TTCCAGATAT TGAAGAAATT 
AGACCAGGCT CAGTGGTCTC CAAGAAAGGA TATCTGCATT TCAAGGAGCC CCTGTCCAGC 
AACTGGGCTA AACATTTCGT CGTGGTCCGT CGCCCTTACG TCTTCATCTA TAACAGCGAC 
AAAGACCCTG TGGAGCGGGG CATCATTAAC CTGTCCACAG CCCAGGTGGA GTATAGCGAG 
GACCAGCAGG CCATGGTGAA GACACCGAAC ACCTTTGCTG TGTGCACCAA GCACCGTGGG 
GTCCTTTTGC AGGCTCTCAA CGACAAAGAC GTGAACGACT GGTTGTACGC CTTTAACCCG 
CTCCTTGCTG GCACAATACG GTCAAAACTC TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015466787.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322273.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.

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CloneID	OBa92947
Clone ID Related Accession (Same CDS sequence)	XM_015475112.1 , XM_015466787.1
Accession Version	XM_015466787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGA CCATGAGAGA AAGATTGAAC TGATCCGATT TCTCGTATCT 
GACTATGTGA ATGTGCATGT GTTGGACACA TTTTCTGAGC ATGCCAACGT GCTTGCCTCC 
TCCGCCATTG CCACCTTCCA GGATGGGGCT GACCCATCAC CGCCTTTTCT CTTTCTGCCA 
GTTCCCAGCT GCCAGCCTGA GAGGGTACCT AAACGAGATG TTCCAGCCAC CAAGTTAAAT 
ACCATGAGCA AAACCAGCCT TGGCCAGAGC ATGAGCAAGT ATGATCTTCT GGTTTGGTTT 
GAGATCAGTG AACTGGAGCC TACGGGAGAG TATATCCCAG CCGTGGTGGA CCATACAGCA 
GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT CACCAGGGCA TCCAGCGGAG GATCACAGTG 
ACCATCATCC ATGAGAAGGG GAGTGAGCTC CACTGGAAGG ATGTTCGTGA GCTGGTGGTA 
GGCCGAATCA GGAATAAGGC TGAGGTGGAT GAAGCTGCAG TGGACGCCAT CCTCTCCCTC 
AATATCATCT CTGCCAAGTA CCTGAAGTCG TCCCACAACT GTAGCAGGAC CTTCTATCGT 
TTCGAGGCCG TCTGGGATAG TTCCCTCCAT AACTCCCTCC TTCTGAACCG AGTGACACCC 
TACGGAGAAA AGATCTACAT GACCCTGTCG GCCTACCTGG AGCTGGATCA CTGCATCCAG 
CCGGCCGTCA TCACCAAGGA CGTGTGCATG GTCTTCTACT CCCGGGACGC CAAGATCTCG 
CCGCCACGCT CGCTGCGCAG CCTCTTTGGC AGCGGCTATT CGAAGTCACC AGACTCCAAT 
CGAGTCACTG GAATTTATGA ACTCAGTTTA TGCAAAATGG CAGACACAGG AAGTCCAGGC 
ATGCAGCGGA GAAGAAGGAA AATCTTGGAT ACCTCAGTGG CGTATGTGCG GGGGGAGGAG 
AACTTGGCTG GCTGGCGGCC CCGGGGAGAC AGCCTCATCC TAGAGCACCA GTGGGAGTTG 
GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG AAAACCCGAC ACTTTCTGCT GCTCCGTGAC 
AGACTGGGTG ACAGCATCCC CAAATCCCTG AGTGACTCGT TGTCCCCCAG CCTCAGCAGC 
GGGACCCTCA GCACCTCCAC CAGCATCTCC TCTCAGATCT CAACCACCAC CTTTGAAAGT 
GCCATCACAC CCAGCGAGAG TAGTGGCTAC GACTCAGCAG ACATTGAAAG CCTGGTGGAT 
CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG CAACTTCTCA CCCACACTTT CAACCGAGAA 
TTCAGCCAGG TGCACGGCAG CATCAGTGAC TGCAAGCTGG CTGACCTCTC CCCCGTCGGC 
CGGGACCCCT CTGTGTCCAG TCTGAGCAGT TCCACCCTCA CGCCATCCTC TACCTGCCCC 
TCTCTGGTGG ATGCTAGGAG CAACTCCCTG GATCAGAAGA CCCCAGAAGC CAATTCCCGG 
GCCTCTAGTC CCTGCCCAGA ATTTGAACAG TTTCAGATTG TCCCACCTGT GGAAACACCC 
TATTTGGCCC GAGCAGGGAA AAATGAATTT CTCAATCTTG TTCCAGATAT TGAAGAAATT 
AGACCAGGCT CAGTGGTCTC CAAGAAAGGA TATCTGCATT TCAAGGAGCC CCTGTCCAGC 
AACTGGGCTA AACATTTCGT CGTGGTCCGT CGCCCTTACG TCTTCATCTA TAACAGCGAC 
AAAGACCCTG TGGAGCGGGG CATCATTAAC CTGTCCACAG CCCAGGTGGA GTATAGCGAG 
GACCAGCAGG CCATGGTGAA GACACCGAAC ACCTTTGCTG TGTGCACCAA GCACCGTGGG 
GTCCTTTTGC AGGCTCTCAA CGACAAAGAC GTGAACGACT GGTTGTACGC CTTTAACCCG 
CTCCTTGCTG GCACAATACG GTCAAAACTC TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322273.1
CDS	388..5790
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015466787.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466787.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGA CCATGAGAGA AAGATTGAAC TGATCCGATT TCTCGTATCT 
GACTATGTGA ATGTGCATGT GTTGGACACA TTTTCTGAGC ATGCCAACGT GCTTGCCTCC 
TCCGCCATTG CCACCTTCCA GGATGGGGCT GACCCATCAC CGCCTTTTCT CTTTCTGCCA 
GTTCCCAGCT GCCAGCCTGA GAGGGTACCT AAACGAGATG TTCCAGCCAC CAAGTTAAAT 
ACCATGAGCA AAACCAGCCT TGGCCAGAGC ATGAGCAAGT ATGATCTTCT GGTTTGGTTT 
GAGATCAGTG AACTGGAGCC TACGGGAGAG TATATCCCAG CCGTGGTGGA CCATACAGCA 
GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT CACCAGGGCA TCCAGCGGAG GATCACAGTG 
ACCATCATCC ATGAGAAGGG GAGTGAGCTC CACTGGAAGG ATGTTCGTGA GCTGGTGGTA 
GGCCGAATCA GGAATAAGGC TGAGGTGGAT GAAGCTGCAG TGGACGCCAT CCTCTCCCTC 
AATATCATCT CTGCCAAGTA CCTGAAGTCG TCCCACAACT GTAGCAGGAC CTTCTATCGT 
TTCGAGGCCG TCTGGGATAG TTCCCTCCAT AACTCCCTCC TTCTGAACCG AGTGACACCC 
TACGGAGAAA AGATCTACAT GACCCTGTCG GCCTACCTGG AGCTGGATCA CTGCATCCAG 
CCGGCCGTCA TCACCAAGGA CGTGTGCATG GTCTTCTACT CCCGGGACGC CAAGATCTCG 
CCGCCACGCT CGCTGCGCAG CCTCTTTGGC AGCGGCTATT CGAAGTCACC AGACTCCAAT 
CGAGTCACTG GAATTTATGA ACTCAGTTTA TGCAAAATGG CAGACACAGG AAGTCCAGGC 
ATGCAGCGGA GAAGAAGGAA AATCTTGGAT ACCTCAGTGG CGTATGTGCG GGGGGAGGAG 
AACTTGGCTG GCTGGCGGCC CCGGGGAGAC AGCCTCATCC TAGAGCACCA GTGGGAGTTG 
GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG AAAACCCGAC ACTTTCTGCT GCTCCGTGAC 
AGACTGGGTG ACAGCATCCC CAAATCCCTG AGTGACTCGT TGTCCCCCAG CCTCAGCAGC 
GGGACCCTCA GCACCTCCAC CAGCATCTCC TCTCAGATCT CAACCACCAC CTTTGAAAGT 
GCCATCACAC CCAGCGAGAG TAGTGGCTAC GACTCAGCAG ACATTGAAAG CCTGGTGGAT 
CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG CAACTTCTCA CCCACACTTT CAACCGAGAA 
TTCAGCCAGG TGCACGGCAG CATCAGTGAC TGCAAGCTGG CTGACCTCTC CCCCGTCGGC 
CGGGACCCCT CTGTGTCCAG TCTGAGCAGT TCCACCCTCA CGCCATCCTC TACCTGCCCC 
TCTCTGGTGG ATGCTAGGAG CAACTCCCTG GATCAGAAGA CCCCAGAAGC CAATTCCCGG 
GCCTCTAGTC CCTGCCCAGA ATTTGAACAG TTTCAGATTG TCCCACCTGT GGAAACACCC 
TATTTGGCCC GAGCAGGGAA AAATGAATTT CTCAATCTTG TTCCAGATAT TGAAGAAATT 
AGACCAGGCT CAGTGGTCTC CAAGAAAGGA TATCTGCATT TCAAGGAGCC CCTGTCCAGC 
AACTGGGCTA AACATTTCGT CGTGGTCCGT CGCCCTTACG TCTTCATCTA TAACAGCGAC 
AAAGACCCTG TGGAGCGGGG CATCATTAAC CTGTCCACAG CCCAGGTGGA GTATAGCGAG 
GACCAGCAGG CCATGGTGAA GACACCGAAC ACCTTTGCTG TGTGCACCAA GCACCGTGGG 
GTCCTTTTGC AGGCTCTCAA CGACAAAGAC GTGAACGACT GGTTGTACGC CTTTAACCCG 
CTCCTTGCTG GCACAATACG GTCAAAACTC TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015466788.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015322274.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.

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CloneID	OBa92948
Clone ID Related Accession (Same CDS sequence)	XM_015466788.1 , XM_015475113.1
Accession Version	XM_015466788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGA CCATGAGAGA AAGATTGAAC TGATCCGATT TCTCGTATCT 
GACTATGTGA ATGTGCATGT GTTGGACACA TTTTCTGAGC ATGCCAACGT GCTTGCCTCC 
TCCGCCATTG CCACCTTCCA GGATGGGGCT GACCCATCAC CGCCTTTTCT CTTTCTGCCA 
GTTCCCAGCT GCCAGCCTGA GAGGGTACCT AAACGAGATG TTCCAGCCAC CAAGTTAAAT 
ACCATGAGCA AAACCAGCCT TGGCCAGAGC ATGAGCAAGT ATGATCTTCT GGTTTGGTTT 
GAGATCAGTG AACTGGAGCC TACGGGAGAG TATATCCCAG CCGTGGTGGA CCATACAGCA 
GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT CACCAGGGCA TCCAGCGGAG GATCACAGTG 
ACCATCATCC ATGAGAAGGG GAGTGAGCTC CACTGGAAGG ATGTTCGTGA GCTGGTGGTA 
GGCCGAATCA GGAATAAGGC TGAGGTGGAT GAAGCTGCAG TGGACGCCAT CCTCTCCCTC 
AATATCATCT CTGCCAAGTA CCTGAAGTCG TCCCACAACT GTAGCAGGAC CTTCTATCGT 
TTCGAGGCCG TCTGGGATAG TTCCCTCCAT AACTCCCTCC TTCTGAACCG AGTGACACCC 
TACGGAGAAA AGATCTACAT GACCCTGTCG GCCTACCTGG AGCTGGATCA CTGCATCCAG 
CCGGCCGTCA TCACCAAGGA CGTGTGCATG GTCTTCTACT CCCGGGACGC CAAGATCTCG 
CCGCCACGCT CGCTGCGCAG CCTCTTTGGC AGCGGCTATT CGAAGTCACC AGACTCCAAT 
CGAGTCACTG GAATTTATGA ACTCAGTTTA TGCAAAATGG CAGACACAGG AAGTCCAGGC 
ATGCAGCGGA GAAGAAGGAA AATCTTGGAT ACCTCAGTGG CGTATGTGCG GGGGGAGGAG 
AACTTGGCTG GCTGGCGGCC CCGGGGAGAC AGCCTCATCC TAGAGCACCA GTGGGAGTTG 
GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG AAAACCCGAC ACTTTCTGCT GCTCCGTGAC 
AGACTGGGTG ACAGCATCCC CAAATCCCTG AGTGACTCGT TGTCCCCCAG CCTCAGCAGC 
GGGACCCTCA GCACCTCCAC CAGCATCTCC TCTCAGATCT CAACCACCAC CTTTGAAAGT 
GCCATCACAC CCAGCGAGAG TAGTGGCTAC GACTCAGCAG ACATTGAAAG CCTGGTGGAT 
CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG CAACTTCTCA CCCACACTTT CAACCGAGAA 
TTCAGCCAGG TGCACGGCAG CATCAGTGAC TGCAAGCTGG CTGACCTCTC CCCCGTCGGC 
CGGGACCCCT CTGTGTCCAG TCTGAGCAGT TCCACCCTCA CGCCATCCTC TACCTGCCCC 
TCTCTGGTGG ATGCTAGGAG CAACTCCCTG GATCAGAAGA CCCCAGAAGC CAATTCCCGG 
GCCTCTAGTC CCTGCCCAGA ATTTGAACAG TTTCAGATTG TCCCACCTGT GGAAACACCC 
TATTTGGCCC GAGCAGGGAA AAATGAATTT CTCAATCTTG TTCCAGATAT TGAAGAAATT 
AGACCAGGCT CAGTGGTCTC CAAGAAAGGA TATCTGCATT TCAAGGAGCC CCTGTCCAGC 
AACTGGGCTA AACATTTCGT CGTGGTCCGT CGCCCTTACG TCTTCATCTA TAACAGCGAC 
AAAGACCCTG TGGAGCGGGG CATCATTAAC CTGTCCACAG CCCAGGTGGA GTATAGCGAG 
GACCAGCAGG CCATGGTGAA GACACCGAAC ACCTTTGCTG TGTGCACCAA GCACCGTGGG 
GTCCTTTTGC AGGCTCTCAA CGACAAAGAC GTGAACGACT GGTTGTACGC CTTTAACCCG 
CTCCTTGCTG GCACAATACG GTCAAAACTC TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015322274.1
CDS	387..5669
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015466788.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466788.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGA CCATGAGAGA AAGATTGAAC TGATCCGATT TCTCGTATCT 
GACTATGTGA ATGTGCATGT GTTGGACACA TTTTCTGAGC ATGCCAACGT GCTTGCCTCC 
TCCGCCATTG CCACCTTCCA GGATGGGGCT GACCCATCAC CGCCTTTTCT CTTTCTGCCA 
GTTCCCAGCT GCCAGCCTGA GAGGGTACCT AAACGAGATG TTCCAGCCAC CAAGTTAAAT 
ACCATGAGCA AAACCAGCCT TGGCCAGAGC ATGAGCAAGT ATGATCTTCT GGTTTGGTTT 
GAGATCAGTG AACTGGAGCC TACGGGAGAG TATATCCCAG CCGTGGTGGA CCATACAGCA 
GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT CACCAGGGCA TCCAGCGGAG GATCACAGTG 
ACCATCATCC ATGAGAAGGG GAGTGAGCTC CACTGGAAGG ATGTTCGTGA GCTGGTGGTA 
GGCCGAATCA GGAATAAGGC TGAGGTGGAT GAAGCTGCAG TGGACGCCAT CCTCTCCCTC 
AATATCATCT CTGCCAAGTA CCTGAAGTCG TCCCACAACT GTAGCAGGAC CTTCTATCGT 
TTCGAGGCCG TCTGGGATAG TTCCCTCCAT AACTCCCTCC TTCTGAACCG AGTGACACCC 
TACGGAGAAA AGATCTACAT GACCCTGTCG GCCTACCTGG AGCTGGATCA CTGCATCCAG 
CCGGCCGTCA TCACCAAGGA CGTGTGCATG GTCTTCTACT CCCGGGACGC CAAGATCTCG 
CCGCCACGCT CGCTGCGCAG CCTCTTTGGC AGCGGCTATT CGAAGTCACC AGACTCCAAT 
CGAGTCACTG GAATTTATGA ACTCAGTTTA TGCAAAATGG CAGACACAGG AAGTCCAGGC 
ATGCAGCGGA GAAGAAGGAA AATCTTGGAT ACCTCAGTGG CGTATGTGCG GGGGGAGGAG 
AACTTGGCTG GCTGGCGGCC CCGGGGAGAC AGCCTCATCC TAGAGCACCA GTGGGAGTTG 
GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG AAAACCCGAC ACTTTCTGCT GCTCCGTGAC 
AGACTGGGTG ACAGCATCCC CAAATCCCTG AGTGACTCGT TGTCCCCCAG CCTCAGCAGC 
GGGACCCTCA GCACCTCCAC CAGCATCTCC TCTCAGATCT CAACCACCAC CTTTGAAAGT 
GCCATCACAC CCAGCGAGAG TAGTGGCTAC GACTCAGCAG ACATTGAAAG CCTGGTGGAT 
CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG CAACTTCTCA CCCACACTTT CAACCGAGAA 
TTCAGCCAGG TGCACGGCAG CATCAGTGAC TGCAAGCTGG CTGACCTCTC CCCCGTCGGC 
CGGGACCCCT CTGTGTCCAG TCTGAGCAGT TCCACCCTCA CGCCATCCTC TACCTGCCCC 
TCTCTGGTGG ATGCTAGGAG CAACTCCCTG GATCAGAAGA CCCCAGAAGC CAATTCCCGG 
GCCTCTAGTC CCTGCCCAGA ATTTGAACAG TTTCAGATTG TCCCACCTGT GGAAACACCC 
TATTTGGCCC GAGCAGGGAA AAATGAATTT CTCAATCTTG TTCCAGATAT TGAAGAAATT 
AGACCAGGCT CAGTGGTCTC CAAGAAAGGA TATCTGCATT TCAAGGAGCC CCTGTCCAGC 
AACTGGGCTA AACATTTCGT CGTGGTCCGT CGCCCTTACG TCTTCATCTA TAACAGCGAC 
AAAGACCCTG TGGAGCGGGG CATCATTAAC CTGTCCACAG CCCAGGTGGA GTATAGCGAG 
GACCAGCAGG CCATGGTGAA GACACCGAAC ACCTTTGCTG TGTGCACCAA GCACCGTGGG 
GTCCTTTTGC AGGCTCTCAA CGACAAAGAC GTGAACGACT GGTTGTACGC CTTTAACCCG 
CTCCTTGCTG GCACAATACG GTCAAAACTC TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_015475113.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330599.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.

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CloneID	OBa92948
Clone ID Related Accession (Same CDS sequence)	XM_015466788.1 , XM_015475113.1
Accession Version	XM_015475113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGA CCATGAGAGA AAGATTGAAC TGATCCGATT TCTCGTATCT 
GACTATGTGA ATGTGCATGT GTTGGACACA TTTTCTGAGC ATGCCAACGT GCTTGCCTCC 
TCCGCCATTG CCACCTTCCA GGATGGGGCT GACCCATCAC CGCCTTTTCT CTTTCTGCCA 
GTTCCCAGCT GCCAGCCTGA GAGGGTACCT AAACGAGATG TTCCAGCCAC CAAGTTAAAT 
ACCATGAGCA AAACCAGCCT TGGCCAGAGC ATGAGCAAGT ATGATCTTCT GGTTTGGTTT 
GAGATCAGTG AACTGGAGCC TACGGGAGAG TATATCCCAG CCGTGGTGGA CCATACAGCA 
GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT CACCAGGGCA TCCAGCGGAG GATCACAGTG 
ACCATCATCC ATGAGAAGGG GAGTGAGCTC CACTGGAAGG ATGTTCGTGA GCTGGTGGTA 
GGCCGAATCA GGAATAAGGC TGAGGTGGAT GAAGCTGCAG TGGACGCCAT CCTCTCCCTC 
AATATCATCT CTGCCAAGTA CCTGAAGTCG TCCCACAACT GTAGCAGGAC CTTCTATCGT 
TTCGAGGCCG TCTGGGATAG TTCCCTCCAT AACTCCCTCC TTCTGAACCG AGTGACACCC 
TACGGAGAAA AGATCTACAT GACCCTGTCG GCCTACCTGG AGCTGGATCA CTGCATCCAG 
CCGGCCGTCA TCACCAAGGA CGTGTGCATG GTCTTCTACT CCCGGGACGC CAAGATCTCG 
CCGCCACGCT CGCTGCGCAG CCTCTTTGGC AGCGGCTATT CGAAGTCACC AGACTCCAAT 
CGAGTCACTG GAATTTATGA ACTCAGTTTA TGCAAAATGG CAGACACAGG AAGTCCAGGC 
ATGCAGCGGA GAAGAAGGAA AATCTTGGAT ACCTCAGTGG CGTATGTGCG GGGGGAGGAG 
AACTTGGCTG GCTGGCGGCC CCGGGGAGAC AGCCTCATCC TAGAGCACCA GTGGGAGTTG 
GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG AAAACCCGAC ACTTTCTGCT GCTCCGTGAC 
AGACTGGGTG ACAGCATCCC CAAATCCCTG AGTGACTCGT TGTCCCCCAG CCTCAGCAGC 
GGGACCCTCA GCACCTCCAC CAGCATCTCC TCTCAGATCT CAACCACCAC CTTTGAAAGT 
GCCATCACAC CCAGCGAGAG TAGTGGCTAC GACTCAGCAG ACATTGAAAG CCTGGTGGAT 
CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG CAACTTCTCA CCCACACTTT CAACCGAGAA 
TTCAGCCAGG TGCACGGCAG CATCAGTGAC TGCAAGCTGG CTGACCTCTC CCCCGTCGGC 
CGGGACCCCT CTGTGTCCAG TCTGAGCAGT TCCACCCTCA CGCCATCCTC TACCTGCCCC 
TCTCTGGTGG ATGCTAGGAG CAACTCCCTG GATCAGAAGA CCCCAGAAGC CAATTCCCGG 
GCCTCTAGTC CCTGCCCAGA ATTTGAACAG TTTCAGATTG TCCCACCTGT GGAAACACCC 
TATTTGGCCC GAGCAGGGAA AAATGAATTT CTCAATCTTG TTCCAGATAT TGAAGAAATT 
AGACCAGGCT CAGTGGTCTC CAAGAAAGGA TATCTGCATT TCAAGGAGCC CCTGTCCAGC 
AACTGGGCTA AACATTTCGT CGTGGTCCGT CGCCCTTACG TCTTCATCTA TAACAGCGAC 
AAAGACCCTG TGGAGCGGGG CATCATTAAC CTGTCCACAG CCCAGGTGGA GTATAGCGAG 
GACCAGCAGG CCATGGTGAA GACACCGAAC ACCTTTGCTG TGTGCACCAA GCACCGTGGG 
GTCCTTTTGC AGGCTCTCAA CGACAAAGAC GTGAACGACT GGTTGTACGC CTTTAACCCG 
CTCCTTGCTG GCACAATACG GTCAAAACTC TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330599.1
CDS	387..5669
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPDHERKIELIRFLVSDYVNVHVLDTFSEHANVLASSAIATFQDGADPSPPFLFLPVPSCQPERVPKRDVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_015475113.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475113.1

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGA CCATGAGAGA AAGATTGAAC TGATCCGATT TCTCGTATCT 
GACTATGTGA ATGTGCATGT GTTGGACACA TTTTCTGAGC ATGCCAACGT GCTTGCCTCC 
TCCGCCATTG CCACCTTCCA GGATGGGGCT GACCCATCAC CGCCTTTTCT CTTTCTGCCA 
GTTCCCAGCT GCCAGCCTGA GAGGGTACCT AAACGAGATG TTCCAGCCAC CAAGTTAAAT 
ACCATGAGCA AAACCAGCCT TGGCCAGAGC ATGAGCAAGT ATGATCTTCT GGTTTGGTTT 
GAGATCAGTG AACTGGAGCC TACGGGAGAG TATATCCCAG CCGTGGTGGA CCATACAGCA 
GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT CACCAGGGCA TCCAGCGGAG GATCACAGTG 
ACCATCATCC ATGAGAAGGG GAGTGAGCTC CACTGGAAGG ATGTTCGTGA GCTGGTGGTA 
GGCCGAATCA GGAATAAGGC TGAGGTGGAT GAAGCTGCAG TGGACGCCAT CCTCTCCCTC 
AATATCATCT CTGCCAAGTA CCTGAAGTCG TCCCACAACT GTAGCAGGAC CTTCTATCGT 
TTCGAGGCCG TCTGGGATAG TTCCCTCCAT AACTCCCTCC TTCTGAACCG AGTGACACCC 
TACGGAGAAA AGATCTACAT GACCCTGTCG GCCTACCTGG AGCTGGATCA CTGCATCCAG 
CCGGCCGTCA TCACCAAGGA CGTGTGCATG GTCTTCTACT CCCGGGACGC CAAGATCTCG 
CCGCCACGCT CGCTGCGCAG CCTCTTTGGC AGCGGCTATT CGAAGTCACC AGACTCCAAT 
CGAGTCACTG GAATTTATGA ACTCAGTTTA TGCAAAATGG CAGACACAGG AAGTCCAGGC 
ATGCAGCGGA GAAGAAGGAA AATCTTGGAT ACCTCAGTGG CGTATGTGCG GGGGGAGGAG 
AACTTGGCTG GCTGGCGGCC CCGGGGAGAC AGCCTCATCC TAGAGCACCA GTGGGAGTTG 
GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG AAAACCCGAC ACTTTCTGCT GCTCCGTGAC 
AGACTGGGTG ACAGCATCCC CAAATCCCTG AGTGACTCGT TGTCCCCCAG CCTCAGCAGC 
GGGACCCTCA GCACCTCCAC CAGCATCTCC TCTCAGATCT CAACCACCAC CTTTGAAAGT 
GCCATCACAC CCAGCGAGAG TAGTGGCTAC GACTCAGCAG ACATTGAAAG CCTGGTGGAT 
CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG CAACTTCTCA CCCACACTTT CAACCGAGAA 
TTCAGCCAGG TGCACGGCAG CATCAGTGAC TGCAAGCTGG CTGACCTCTC CCCCGTCGGC 
CGGGACCCCT CTGTGTCCAG TCTGAGCAGT TCCACCCTCA CGCCATCCTC TACCTGCCCC 
TCTCTGGTGG ATGCTAGGAG CAACTCCCTG GATCAGAAGA CCCCAGAAGC CAATTCCCGG 
GCCTCTAGTC CCTGCCCAGA ATTTGAACAG TTTCAGATTG TCCCACCTGT GGAAACACCC 
TATTTGGCCC GAGCAGGGAA AAATGAATTT CTCAATCTTG TTCCAGATAT TGAAGAAATT 
AGACCAGGCT CAGTGGTCTC CAAGAAAGGA TATCTGCATT TCAAGGAGCC CCTGTCCAGC 
AACTGGGCTA AACATTTCGT CGTGGTCCGT CGCCCTTACG TCTTCATCTA TAACAGCGAC 
AAAGACCCTG TGGAGCGGGG CATCATTAAC CTGTCCACAG CCCAGGTGGA GTATAGCGAG 
GACCAGCAGG CCATGGTGAA GACACCGAAC ACCTTTGCTG TGTGCACCAA GCACCGTGGG 
GTCCTTTTGC AGGCTCTCAA CGACAAAGAC GTGAACGACT GGTTGTACGC CTTTAACCCG 
CTCCTTGCTG GCACAATACG GTCAAAACTC TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216992.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217049.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.

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CloneID	OBa27227
Clone ID Related Accession (Same CDS sequence)	XM_005216992.3 , XM_010823090.2
Accession Version	XM_005216992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGACAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217049.1
CDS	388..5838
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_005216992.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216992.3

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGACAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_010823090.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821392.2 Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.

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CloneID	OBa27227
Clone ID Related Accession (Same CDS sequence)	XM_005216992.3 , XM_010823090.2
Accession Version	XM_010823090.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGACAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821392.2
CDS	388..5838
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKYLKDFQNNKHRYLLASENQRPGNFSTASMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_010823090.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823090.2

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATATCTGAAA GATTTTCAGA ACAATAAGCA TAGGTACTTG 
CTAGCCTCTG AGAATCAACG TCCTGGCAAT TTTTCCACAG CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGACAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_010823091.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821393.2 Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.

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CloneID	OBa27228
Clone ID Related Accession (Same CDS sequence)	XM_010823091.2 , XM_005216993.3
Accession Version	XM_010823091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGAC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821393.2
CDS	388..5760
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKSSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_010823091.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823091.2

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGAC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216993.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217050.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.

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CloneID	OBa27228
Clone ID Related Accession (Same CDS sequence)	XM_010823091.2 , XM_005216993.3
Accession Version	XM_005216993.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216993.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGAC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217050.1
CDS	388..5760
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDIDPLIDDYSGSGGKSSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_005216993.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216993.3

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA TTGATCCATT GATCGATGAT 
TACTCGGGAA GTGGAGGCAA ATCATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGAC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_002694045.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002694091.3 Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa27230
Clone ID Related Accession (Same CDS sequence)	XM_002694045.5 , XM_010823092.2
Accession Version	XM_002694045.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002694045.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGACAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002694091.3
CDS	387..5717
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_002694045.5
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002694045.5

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGACAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_010823092.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821394.2 Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.

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CloneID	OBa27230
Clone ID Related Accession (Same CDS sequence)	XM_002694045.5 , XM_010823092.2
Accession Version	XM_010823092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGACAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821394.2
CDS	387..5717
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_010823092.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823092.2

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGCTCCC CCATTTTCCA CGGCTGTGTG 
AATGAGCGCC TTGCTGACCG CACACCCTCC CCCACTTTTT CCACGGCCGA TTCCGACATC 
ACTGAGCTGG CTGACGAGCA GCAAGAGGAG ATGGAGGACT TTGATGAGGA GGCGTTCGTG 
GATGACACCG GCTCTGACGC AGGGACGGAG GAGGGCTCAG ATCTCTTCAG TGACGGGCAC 
GACCCGTTTT ACGACCGGTC CCCATGGTTC ATTTTAGTGG GAAGGGCCTT TGTTTACCTG 
AGCAACCTGC TGTACCCCGT GCCCCTGATT CACAGGGTGG CCATCGTCAG TGAGAAGGGA 
GAGGTGCGTG GATTTCTGCG TGTGGCTGTG CAGGCCATTG CAGCGGATGA AGAAGCTCCT 
GATTATGGCT CTGGAATTCG ACAGTCAGGA ACAGCTAAAA TATCTTTTGA TAACGAGTAC 
TTTAATCAGA GTGACGTTTC TTCGGTTGCA TTGACCCGTT CTGGTCTGTC CTTGGAGGAG 
CTGAGGATTG TGGAAGGACA GGGTCAGAGT TCTGAGGTCA TCACTCCTCC AGAAGAAGTC 
AACCGAATGA ATGATCTGGA TCTGAAGTCA AGCACTTTGC TGGATGGTAA GATGATGATG 
GAGGGATTTT CTGAAGAGAT CGGCAACCAC CTAAAGCTGG GCAGTGCATT CACTTTCCGG 
GTCACAGTGC TGCAGGCCAG TGGAATCCTC CCAGAGTATG CAGACATCTT CTGTCAGTTC 
AACTTTTTAC ATCGGCATGA TGAAGCATTC TCCACTGAAC CCCTCAAGAA CAACGGCAGA 
GGAAGTCCTC TGGGCTTTTA CCATGTACAG AATATTGCAG TGGAGGTTAC CGAATCATTC 
GTGGACTATA TCAAAACCAA GCCGATTGTG TTTGAAGTCT TTGGGCATTA TCAGCAGCAC 
CCACTTCACC TGCAAGGACA GGAACTTCAC AGTCCACCCC AGCCGTCTCG ACGATTCTTC 
CCTCCGCCCA TGCCGCTATC CAAGCCAGTT CCAGCCACCA AGTTAAATAC CATGAGCAAA 
ACCAGCCTTG GCCAGAGCAT GAGCAAGTAT GATCTTCTGG TTTGGTTTGA GATCAGTGAA 
CTGGAGCCTA CGGGAGAGTA TATCCCAGCC GTGGTGGACC ATACAGCAGG CTTGCCCTGC 
CAGGGGACGT TTTTGCTTCA CCAGGGCATC CAGCGGAGGA TCACAGTGAC CATCATCCAT 
GAGAAGGGGA GTGAGCTCCA CTGGAAGGAT GTTCGTGAGC TGGTGGTAGG CCGAATCAGG 
AATAAGGCTG AGGTGGATGA AGCTGCAGTG GACGCCATCC TCTCCCTCAA TATCATCTCT 
GCCAAGTACC TGAAGTCGTC CCACAACTGT AGCAGGACCT TCTATCGTTT CGAGGCCGTC 
TGGGATAGTT CCCTCCATAA CTCCCTCCTT CTGAACCGAG TGACACCCTA CGGAGAAAAG 
ATCTACATGA CCCTGTCGGC CTACCTGGAG CTGGATCACT GCATCCAGCC GGCCGTCATC 
ACCAAGGACG TGTGCATGGT CTTCTACTCC CGGGACGCCA AGATCTCGCC GCCACGCTCG 
CTGCGCAGCC TCTTTGGCAG CGGCTATTCG AAGTCACCAG ACTCCAATCG AGTCACTGGA 
ATTTATGAAC TCAGTTTATG CAAAATGGCA GACACAGGAA GTCCAGGCAT GCAGCGGAGA 
AGAAGGAAAA TCTTGGATAC CTCAGTGGCG TATGTGCGGG GGGAGGAGAA CTTGGCTGGC 
TGGCGGCCCC GGGGAGACAG CCTCATCCTA GAGCACCAGT GGGAGTTGGA GAAGCTGGAG 
CTCCTGCACG AGGTGGAGAA AACCCGACAC TTTCTGCTGC TCCGTGACAG ACTGGGTGAC 
AGCATCCCCA AATCCCTGAG TGACTCGTTG TCCCCCAGCC TCAGCAGCGG GACCCTCAGC 
ACCTCCACCA GCATCTCCTC TCAGATCTCA ACCACCACCT TTGAAAGTGC CATCACACCC 
AGCGAGAGTA GTGGCTACGA CTCAGCAGAC ATTGAAAGCC TGGTGGATCG AGAGAAAGAG 
CTGGCTACAA AGTGCCTGCA ACTTCTCACC CACACTTTCA ACCGAGAATT CAGCCAGGTG 
CACGGCAGCA TCAGTGACTG CAAGCTGGCT GACCTCTCCC CCGTCGGCCG GGACCCCTCT 
GTGTCCAGTC TGAGCAGTTC CACCCTCACG CCATCCTCTA CCTGCCCCTC TCTGGTGGAT 
GCTAGGAGCA ACTCCCTGGA TCAGAAGACC CCAGAAGCCA ATTCCCGGGC CTCTAGTCCC 
TGCCCAGAAT TTGAACAGTT TCAGATTGTC CCACCTGTGG AAACACCCTA TTTGGCCCGA 
GCAGGGAAAA ATGAATTTCT CAATCTTGTT CCAGATATTG AAGAAATTAG ACCAGGCTCA 
GTGGTCTCCA AGAAAGGATA TCTGCATTTC AAGGAGCCCC TGTCCAGCAA CTGGGCTAAA 
CATTTCGTCG TGGTCCGTCG CCCTTACGTC TTCATCTATA ACAGCGACAA AGACCCTGTG 
GAGCGGGGCA TCATTAACCT GTCCACAGCC CAGGTGGAGT ATAGCGAGGA CCAGCAGGCC 
ATGGTGAAGA CACCGAACAC CTTTGCTGTG TGCACCAAGC ACCGTGGGGT CCTTTTGCAG 
GCTCTCAACG ACAAAGACGT GAACGACTGG TTGTACGCCT TTAACCCGCT CCTTGCTGGC 
ACAATACGGT CAAAACTCTC CCGCAGATGC CCGAGCCAAG CGAAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_003583233.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583281.3 Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.

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CloneID	OBa27231
Clone ID Related Accession (Same CDS sequence)	XM_003583233.4 , XM_005216995.3
Accession Version	XM_003583233.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583233.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGAC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583281.3
CDS	387..5699
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_003583233.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583233.4

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGAC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216995.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217052.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.

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CloneID	OBa27231
Clone ID Related Accession (Same CDS sequence)	XM_003583233.4 , XM_005216995.3
Accession Version	XM_005216995.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5313bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGAC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217052.1
CDS	387..5699
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQEEMEDFDEEAFVDDTGSDAGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_005216995.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216995.3

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGAGTAAG AAGAAGAAGA AAACTGATTT TATTCCCTAC 
AGAGATTCTG TACTTACTTG GCTCCTTCGA GAAAATCTAG GTGGCAATTC TCGGACTGCA 
ATGGTTGCTG CTCTGAGCCC AGCTGATATC AACTATGATG AAACTTTGAG CACTCTGAGA 
TACGCAGATC GTGCAAAACA AATTAAATGT AATGCTGTTA TCAATGAGGA CCCCAATGCC 
AAACTGGTTC GTGAATTAAA GGAGGAGGTG ACACGGCTGA AGGACCTTCT TCGTGCTCAG 
GGGCTGGGAG ATATTATTGA TACATCCATG GGGTCCCTTA CTTCATCTCC ATCTTCCTGC 
TCACTCAATA GTCAGGTGGG CTTAACATCT GTGACCAGTA TTCAGGAGAG AATCATGTCT 
ACACCTGGAG GAGAGGAAGC CATCGAACGT CTGAAGGAAT CAGAGAAGAT CATTGCTGAG 
TTGAATGAAA CCTGGGAAGA GAAGCTGCGT AAAACAGAGG CCATCAGGAT GGAGAGAGAA 
GCCTTATTGG CTGAGATGGG AGTTGCCATT CGGGAAGACG GAGGAACCCT TGGGGTTTTC 
TCTCCTAAAA AGACACCACA TCTTGTTAAC CTCAATGAGG ACCCTCTAAT GTCCGAGTGC 
CTGCTTTATT ACATCAAAGA TGGAATTACA AGGGTTGGCC AAGCAGATGC TGAGCGGCGC 
CAGGACATCG TGCTGAGTGG GGCTCACATT AAAGAAGAGC ATTGTATCTT CCGGAGCGAG 
AGGAACAACA GCGGGGATGT TATTGTGACT CTGGAGCCCT GTGAACGTTC AGAAACCTAC 
GTAAATGGCA AGAGGGTGAC CCAGCCTGTT CAGCTGCGCT CAGGAAACCG TATCATCATG 
GGTAAAAACC ACGTGTTCCG TTTTAACCAC CCGGAGCAAG CACGGGCTGA GCGGGAAAAG 
ACTCCTTCTG CTGAGACCCC CTCTGAGCCT GTGGACTGGA CGTTTGCCCA GAGAGAGCTT 
CTGGAAAAAC AAGGAATTGA TATGAAACAG GAGATGGAAA AAAGGCTACA GGAAATGGAG 
ATCCTATACA AAAAGGAGAA GGAAGAAGCA GATCTTCTCT TGGAGCAGCA GAGACTGGAC 
TATGAGAGTA AATTGCAGGC CTTGCAGAAG CAGGTGGAGA CGCGATCCCT GGCTGCAGAA 
ACAACTGAAG AGGAAGAGGA GGAGGAAGAA GTTCCGTGGA CACAACATGA ATTTGAGCTG 
GCCCAGTGGG CCTTCCGGAA GTGGAAGTCT CACCAATTCA CCTCATTAAG GGACTTACTC 
TGGGGCAATG CCGTTTACCT AAAGGAGGCC AATGCCATCA GTGTGGAACT AAAGAAGAAG 
GTGCAGTTTC AGTTTGTTCT GCTCACGGAC ACGTTGTACT CCCCTCTGCC TCCTGAACTG 
CTTCCCACTG AGATTGAAAA AACTCAGGAA GACAGACCTT TCCCTCGTAC AGTGGTAGCT 
GTAGAAGTTC AGGATCTGAA AAATGGAGCA ACACATTATT GGTCTTTGGA GAAACTTAAG 
CAGAGGCTGG ACTTGATGAG AGAGATGTAT GACAGAGCAG GGGAGATGGC CTCCAGCGCC 
CAAGATGAAA GTGAGACCAC CATGACTGGC AGCGATCCAT TTTATGATCG GTTCCATTGG 
TTCAAACTTG TGGGAAGCTC CCCCATTTTC CACGGCTGTG TGAATGAGCG CCTTGCTGAC 
CGCACACCCT CCCCCACTTT TTCCACGGCC GATTCCGACA TCACTGAGCT GGCTGACGAG 
CAGCAAGAGG AGATGGAGGA CTTTGATGAG GAGGCGTTCG TGGATGACAC CGGCTCTGAC 
GCAGGGACGG AGGAGGGCTC AGATCTCTTC AGTGACGGGC ACGACCCGTT TTACGACCGG 
TCCCCATGGT TCATTTTAGT GGGAAGGGCC TTTGTTTACC TGAGCAACCT GCTGTACCCC 
GTGCCCCTGA TTCACAGGGT GGCCATCGTC AGTGAGAAGG GAGAGGTGCG TGGATTTCTG 
CGTGTGGCTG TGCAGGCCAT TGCAGCGGAT GAAGAAGCTC CTGATTATGG CTCTGGAATT 
CGACAGTCAG GAACAGCTAA AATATCTTTT GATAACGAGT ACTTTAATCA GAGTGACGTT 
TCTTCGGTTG CATTGACCCG TTCTGGTCTG TCCTTGGAGG AGCTGAGGAT TGTGGAAGGA 
CAGGGTCAGA GTTCTGAGGT CATCACTCCT CCAGAAGAAG TCAACCGAAT GAATGATCTG 
GATCTGAAGT CAAGCACTTT GCTGGATGGT AAGATGATGA TGGAGGGATT TTCTGAAGAG 
ATCGGCAACC ACCTAAAGCT GGGCAGTGCA TTCACTTTCC GGGTCACAGT GCTGCAGGCC 
AGTGGAATCC TCCCAGAGTA TGCAGACATC TTCTGTCAGT TCAACTTTTT ACATCGGCAT 
GATGAAGCAT TCTCCACTGA ACCCCTCAAG AACAACGGCA GAGGAAGTCC TCTGGGCTTT 
TACCATGTAC AGAATATTGC AGTGGAGGTT ACCGAATCAT TCGTGGACTA TATCAAAACC 
AAGCCGATTG TGTTTGAAGT CTTTGGGCAT TATCAGCAGC ACCCACTTCA CCTGCAAGGA 
CAGGAACTTC ACAGTCCACC CCAGCCGTCT CGACGATTCT TCCCTCCGCC CATGCCGCTA 
TCCAAGCCAG TTCCAGCCAC CAAGTTAAAT ACCATGAGCA AAACCAGCCT TGGCCAGAGC 
ATGAGCAAGT ATGATCTTCT GGTTTGGTTT GAGATCAGTG AACTGGAGCC TACGGGAGAG 
TATATCCCAG CCGTGGTGGA CCATACAGCA GGCTTGCCCT GCCAGGGGAC GTTTTTGCTT 
CACCAGGGCA TCCAGCGGAG GATCACAGTG ACCATCATCC ATGAGAAGGG GAGTGAGCTC 
CACTGGAAGG ATGTTCGTGA GCTGGTGGTA GGCCGAATCA GGAATAAGGC TGAGGTGGAT 
GAAGCTGCAG TGGACGCCAT CCTCTCCCTC AATATCATCT CTGCCAAGTA CCTGAAGTCG 
TCCCACAACT GTAGCAGGAC CTTCTATCGT TTCGAGGCCG TCTGGGATAG TTCCCTCCAT 
AACTCCCTCC TTCTGAACCG AGTGACACCC TACGGAGAAA AGATCTACAT GACCCTGTCG 
GCCTACCTGG AGCTGGATCA CTGCATCCAG CCGGCCGTCA TCACCAAGGA CGTGTGCATG 
GTCTTCTACT CCCGGGACGC CAAGATCTCG CCGCCACGCT CGCTGCGCAG CCTCTTTGGC 
AGCGGCTATT CGAAGTCACC AGACTCCAAT CGAGTCACTG GAATTTATGA ACTCAGTTTA 
TGCAAAATGG CAGACACAGG AAGTCCAGGC ATGCAGCGGA GAAGAAGGAA AATCTTGGAT 
ACCTCAGTGG CGTATGTGCG GGGGGAGGAG AACTTGGCTG GCTGGCGGCC CCGGGGAGAC 
AGCCTCATCC TAGAGCACCA GTGGGAGTTG GAGAAGCTGG AGCTCCTGCA CGAGGTGGAG 
AAAACCCGAC ACTTTCTGCT GCTCCGTGAC AGACTGGGTG ACAGCATCCC CAAATCCCTG 
AGTGACTCGT TGTCCCCCAG CCTCAGCAGC GGGACCCTCA GCACCTCCAC CAGCATCTCC 
TCTCAGATCT CAACCACCAC CTTTGAAAGT GCCATCACAC CCAGCGAGAG TAGTGGCTAC 
GACTCAGCAG ACATTGAAAG CCTGGTGGAT CGAGAGAAAG AGCTGGCTAC AAAGTGCCTG 
CAACTTCTCA CCCACACTTT CAACCGAGAA TTCAGCCAGG TGCACGGCAG CATCAGTGAC 
TGCAAGCTGG CTGACCTCTC CCCCGTCGGC CGGGACCCCT CTGTGTCCAG TCTGAGCAGT 
TCCACCCTCA CGCCATCCTC TACCTGCCCC TCTCTGGTGG ATGCTAGGAG CAACTCCCTG 
GATCAGAAGA CCCCAGAAGC CAATTCCCGG GCCTCTAGTC CCTGCCCAGA ATTTGAACAG 
TTTCAGATTG TCCCACCTGT GGAAACACCC TATTTGGCCC GAGCAGGGAA AAATGAATTT 
CTCAATCTTG TTCCAGATAT TGAAGAAATT AGACCAGGCT CAGTGGTCTC CAAGAAAGGA 
TATCTGCATT TCAAGGAGCC CCTGTCCAGC AACTGGGCTA AACATTTCGT CGTGGTCCGT 
CGCCCTTACG TCTTCATCTA TAACAGCGAC AAAGACCCTG TGGAGCGGGG CATCATTAAC 
CTGTCCACAG CCCAGGTGGA GTATAGCGAG GACCAGCAGG CCATGGTGAA GACACCGAAC 
ACCTTTGCTG TGTGCACCAA GCACCGTGGG GTCCTTTTGC AGGCTCTCAA CGACAAAGAC 
GTGAACGACT GGTTGTACGC CTTTAACCCG CTCCTTGCTG GCACAATACG GTCAAAACTC 
TCCCGCAGAT GCCCGAGCCA AGCGAAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_010823094.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010821396.2 Bos taurus kinesin family member 1B (KIF1B), transcript variant X14, mRNA.

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CloneID	OBa27233
Clone ID Related Accession (Same CDS sequence)	XM_010823094.2 , XM_005216997.3
Accession Version	XM_010823094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646322.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGT TCCAGCCACC AAGTTAAATA CCATGAGCAA AACCAGCCTT 
GGCCAGAGCA TGAGCAAGTA TGATCTTCTG GTTTGGTTTG AGATCAGTGA ACTGGAGCCT 
ACGGGAGAGT ATATCCCAGC CGTGGTGGAC CATACAGCAG GCTTGCCCTG CCAGGGGACG 
TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG ATCACAGTGA CCATCATCCA TGAGAAGGGG 
AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG CTGGTGGTAG GCCGAATCAG GAATAAGGCT 
GAGGTGGATG AAGCTGCAGT GGACGCCATC CTCTCCCTCA ATATCATCTC TGCCAAGTAC 
CTGAAGTCGT CCCACAACTG TAGCAGGACC TTCTATCGTT TCGAGGCCGT CTGGGATAGT 
TCCCTCCATA ACTCCCTCCT TCTGAACCGA GTGACACCCT ACGGAGAAAA GATCTACATG 
ACCCTGTCGG CCTACCTGGA GCTGGATCAC TGCATCCAGC CGGCCGTCAT CACCAAGGAC 
GTGTGCATGG TCTTCTACTC CCGGGACGCC AAGATCTCGC CGCCACGCTC GCTGCGCAGC 
CTCTTTGGCA GCGGCTATTC GAAGTCACCA GACTCCAATC GAGTCACTGG AATTTATGAA 
CTCAGTTTAT GCAAAATGGC AGACACAGGA AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA 
ATCTTGGATA CCTCAGTGGC GTATGTGCGG GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC 
CGGGGAGACA GCCTCATCCT AGAGCACCAG TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC 
GAGGTGGAGA AAACCCGACA CTTTCTGCTG CTCCGTGACA GACTGGGTGA CAGCATCCCC 
AAATCCCTGA GTGACTCGTT GTCCCCCAGC CTCAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CTCAGATCTC AACCACCACC TTTGAAAGTG CCATCACACC CAGCGAGAGT 
AGTGGCTACG ACTCAGCAGA CATTGAAAGC CTGGTGGATC GAGAGAAAGA GCTGGCTACA 
AAGTGCCTGC AACTTCTCAC CCACACTTTC AACCGAGAAT TCAGCCAGGT GCACGGCAGC 
ATCAGTGACT GCAAGCTGGC TGACCTCTCC CCCGTCGGCC GGGACCCCTC TGTGTCCAGT 
CTGAGCAGTT CCACCCTCAC GCCATCCTCT ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC 
AACTCCCTGG ATCAGAAGAC CCCAGAAGCC AATTCCCGGG CCTCTAGTCC CTGCCCAGAA 
TTTGAACAGT TTCAGATTGT CCCACCTGTG GAAACACCCT ATTTGGCCCG AGCAGGGAAA 
AATGAATTTC TCAATCTTGT TCCAGATATT GAAGAAATTA GACCAGGCTC AGTGGTCTCC 
AAGAAAGGAT ATCTGCATTT CAAGGAGCCC CTGTCCAGCA ACTGGGCTAA ACATTTCGTC 
GTGGTCCGTC GCCCTTACGT CTTCATCTAT AACAGCGACA AAGACCCTGT GGAGCGGGGC 
ATCATTAACC TGTCCACAGC CCAGGTGGAG TATAGCGAGG ACCAGCAGGC CATGGTGAAG 
ACACCGAACA CCTTTGCTGT GTGCACCAAG CACCGTGGGG TCCTTTTGCA GGCTCTCAAC 
GACAAAGACG TGAACGACTG GTTGTACGCC TTTAACCCGC TCCTTGCTGG CACAATACGG 
TCAAAACTCT CCCGCAGATG CCCGAGCCAA GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010821396.2
CDS	386..5467
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_010823094.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823094.2

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGT TCCAGCCACC AAGTTAAATA CCATGAGCAA AACCAGCCTT 
GGCCAGAGCA TGAGCAAGTA TGATCTTCTG GTTTGGTTTG AGATCAGTGA ACTGGAGCCT 
ACGGGAGAGT ATATCCCAGC CGTGGTGGAC CATACAGCAG GCTTGCCCTG CCAGGGGACG 
TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG ATCACAGTGA CCATCATCCA TGAGAAGGGG 
AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG CTGGTGGTAG GCCGAATCAG GAATAAGGCT 
GAGGTGGATG AAGCTGCAGT GGACGCCATC CTCTCCCTCA ATATCATCTC TGCCAAGTAC 
CTGAAGTCGT CCCACAACTG TAGCAGGACC TTCTATCGTT TCGAGGCCGT CTGGGATAGT 
TCCCTCCATA ACTCCCTCCT TCTGAACCGA GTGACACCCT ACGGAGAAAA GATCTACATG 
ACCCTGTCGG CCTACCTGGA GCTGGATCAC TGCATCCAGC CGGCCGTCAT CACCAAGGAC 
GTGTGCATGG TCTTCTACTC CCGGGACGCC AAGATCTCGC CGCCACGCTC GCTGCGCAGC 
CTCTTTGGCA GCGGCTATTC GAAGTCACCA GACTCCAATC GAGTCACTGG AATTTATGAA 
CTCAGTTTAT GCAAAATGGC AGACACAGGA AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA 
ATCTTGGATA CCTCAGTGGC GTATGTGCGG GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC 
CGGGGAGACA GCCTCATCCT AGAGCACCAG TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC 
GAGGTGGAGA AAACCCGACA CTTTCTGCTG CTCCGTGACA GACTGGGTGA CAGCATCCCC 
AAATCCCTGA GTGACTCGTT GTCCCCCAGC CTCAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CTCAGATCTC AACCACCACC TTTGAAAGTG CCATCACACC CAGCGAGAGT 
AGTGGCTACG ACTCAGCAGA CATTGAAAGC CTGGTGGATC GAGAGAAAGA GCTGGCTACA 
AAGTGCCTGC AACTTCTCAC CCACACTTTC AACCGAGAAT TCAGCCAGGT GCACGGCAGC 
ATCAGTGACT GCAAGCTGGC TGACCTCTCC CCCGTCGGCC GGGACCCCTC TGTGTCCAGT 
CTGAGCAGTT CCACCCTCAC GCCATCCTCT ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC 
AACTCCCTGG ATCAGAAGAC CCCAGAAGCC AATTCCCGGG CCTCTAGTCC CTGCCCAGAA 
TTTGAACAGT TTCAGATTGT CCCACCTGTG GAAACACCCT ATTTGGCCCG AGCAGGGAAA 
AATGAATTTC TCAATCTTGT TCCAGATATT GAAGAAATTA GACCAGGCTC AGTGGTCTCC 
AAGAAAGGAT ATCTGCATTT CAAGGAGCCC CTGTCCAGCA ACTGGGCTAA ACATTTCGTC 
GTGGTCCGTC GCCCTTACGT CTTCATCTAT AACAGCGACA AAGACCCTGT GGAGCGGGGC 
ATCATTAACC TGTCCACAGC CCAGGTGGAG TATAGCGAGG ACCAGCAGGC CATGGTGAAG 
ACACCGAACA CCTTTGCTGT GTGCACCAAG CACCGTGGGG TCCTTTTGCA GGCTCTCAAC 
GACAAAGACG TGAACGACTG GTTGTACGCC TTTAACCCGC TCCTTGCTGG CACAATACGG 
TCAAAACTCT CCCGCAGATG CCCGAGCCAA GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1B ( XM_005216997.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217054.1 Bos taurus kinesin family member 1B (KIF1B), transcript variant X14, mRNA.

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CloneID	OBa27233
Clone ID Related Accession (Same CDS sequence)	XM_010823094.2 , XM_005216997.3
Accession Version	XM_005216997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGT TCCAGCCACC AAGTTAAATA CCATGAGCAA AACCAGCCTT 
GGCCAGAGCA TGAGCAAGTA TGATCTTCTG GTTTGGTTTG AGATCAGTGA ACTGGAGCCT 
ACGGGAGAGT ATATCCCAGC CGTGGTGGAC CATACAGCAG GCTTGCCCTG CCAGGGGACG 
TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG ATCACAGTGA CCATCATCCA TGAGAAGGGG 
AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG CTGGTGGTAG GCCGAATCAG GAATAAGGCT 
GAGGTGGATG AAGCTGCAGT GGACGCCATC CTCTCCCTCA ATATCATCTC TGCCAAGTAC 
CTGAAGTCGT CCCACAACTG TAGCAGGACC TTCTATCGTT TCGAGGCCGT CTGGGATAGT 
TCCCTCCATA ACTCCCTCCT TCTGAACCGA GTGACACCCT ACGGAGAAAA GATCTACATG 
ACCCTGTCGG CCTACCTGGA GCTGGATCAC TGCATCCAGC CGGCCGTCAT CACCAAGGAC 
GTGTGCATGG TCTTCTACTC CCGGGACGCC AAGATCTCGC CGCCACGCTC GCTGCGCAGC 
CTCTTTGGCA GCGGCTATTC GAAGTCACCA GACTCCAATC GAGTCACTGG AATTTATGAA 
CTCAGTTTAT GCAAAATGGC AGACACAGGA AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA 
ATCTTGGATA CCTCAGTGGC GTATGTGCGG GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC 
CGGGGAGACA GCCTCATCCT AGAGCACCAG TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC 
GAGGTGGAGA AAACCCGACA CTTTCTGCTG CTCCGTGACA GACTGGGTGA CAGCATCCCC 
AAATCCCTGA GTGACTCGTT GTCCCCCAGC CTCAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CTCAGATCTC AACCACCACC TTTGAAAGTG CCATCACACC CAGCGAGAGT 
AGTGGCTACG ACTCAGCAGA CATTGAAAGC CTGGTGGATC GAGAGAAAGA GCTGGCTACA 
AAGTGCCTGC AACTTCTCAC CCACACTTTC AACCGAGAAT TCAGCCAGGT GCACGGCAGC 
ATCAGTGACT GCAAGCTGGC TGACCTCTCC CCCGTCGGCC GGGACCCCTC TGTGTCCAGT 
CTGAGCAGTT CCACCCTCAC GCCATCCTCT ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC 
AACTCCCTGG ATCAGAAGAC CCCAGAAGCC AATTCCCGGG CCTCTAGTCC CTGCCCAGAA 
TTTGAACAGT TTCAGATTGT CCCACCTGTG GAAACACCCT ATTTGGCCCG AGCAGGGAAA 
AATGAATTTC TCAATCTTGT TCCAGATATT GAAGAAATTA GACCAGGCTC AGTGGTCTCC 
AAGAAAGGAT ATCTGCATTT CAAGGAGCCC CTGTCCAGCA ACTGGGCTAA ACATTTCGTC 
GTGGTCCGTC GCCCTTACGT CTTCATCTAT AACAGCGACA AAGACCCTGT GGAGCGGGGC 
ATCATTAACC TGTCCACAGC CCAGGTGGAG TATAGCGAGG ACCAGCAGGC CATGGTGAAG 
ACACCGAACA CCTTTGCTGT GTGCACCAAG CACCGTGGGG TCCTTTTGCA GGCTCTCAAC 
GACAAAGACG TGAACGACTG GTTGTACGCC TTTAACCCGC TCCTTGCTGG CACAATACGG 
TCAAAACTCT CCCGCAGATG CCCGAGCCAA GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217054.1
CDS	386..5467
Translation	MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGHLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDTETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLNSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERNNSGDVIVTLEPCERSETYVNGKRVTQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLLWGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEIEKTQEDRPFPRTVVAVEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTMTGSDPFYDRFHWFKLVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFLRVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDVSSVALTRSGLSLEELRIVEGQGQSSEVITPPEEVNRMNDLDLKSSTLLDGKMMMEGFSEEIGNHLKLGSAFTFRVTVLQASGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLGFYHVQNIAVEVTESFVDYIKTKPIVFEVFGHYQQHPLHLQGQELHSPPQPSRRFFPPPMPLSKPVPATKLNTMSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKAEVDEAAVDAILSLNIISAKYLKSSHNCSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRDRLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSADIESLVDREKELATKCLQLLTHTFNREFSQVHGSISDCKLADLSPVGRDPSVSSLSSSTLTPSSTCPSLVDARSNSLDQKTPEANSRASSPCPEFEQFQIVPPVETPYLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLHFKEPLSSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDVNDWLYAFNPLLAGTIRSKLSRRCPSQAKY

Target ORF information:

RefSeq Version	XM_005216997.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1B (KIF1B), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216997.3

ORF Insert Sequence:

ATGTCGGGAG CCTCAGTGAA GGTGGCTGTC CGTGTTCGGC CCTTCAATTC TCGAGAGACC 
AGCAAAGAGT CCAAGTGCAT CATTCAGATG CAGGGCAACT CAACCAGTAT TATTAACCCA 
AAGAACCCAA AGGAAGCTCC AAAGTCTTTC AGCTTTGACT ACTCCTACTG GTCTCACACC 
TCGCCCGAAG ATCCCTGTTT TGCATCTCAA AACCGTGTAT ACAATGATAT TGGAAAGGAA 
ATGCTCTTAC ATGCCTTTGA GGGATATAAT GTCTGTATTT TTGCCTATGG GCAGACTGGT 
GCTGGAAAGT CTTATACAAT GATGGGTAAA CAAGAAGAAA GCCAGGCTGG CATCATTCCA 
CAGTTATGTG AAGAGCTTTT TGAGAAAATC AATGACAACT GTAACGAAGA AATGTCTTAC 
TCTGTAGAGG TGAGCTACAT GGAAATTTAC TGTGAAAGAG TACGAGACTT GCTGAATCCC 
AAAAACAAGG GTCATCTGCG TGTGCGTGAA CATCCCCTTC TTGGCCCTTA TGTGGAGGAC 
CTGTCAAAGT TGGCAGTCAC TTCCTACACA GACATTGCTG ACCTCATGGA CGCTGGGAAC 
AAAGCCAGGA CAGTGGCAGC TACCAACATG AATGAAACAA GTAGCCGTTC CCACGCTGTG 
TTTACCATTG TCTTCACCCA GAAGAAACAT GATACTGAGA CCAACCTTTC CACTGAGAAG 
GTCAGTAAAA TTAGCTTGGT GGATCTAGCA GGAAGTGAAC GAGCTGATTC AACTGGTGCC 
AAAGGGACTC GATTAAAGGA AGGAGCAAAT ATTAATAAGT CTCTTACAAC TCTGGGTAAA 
GTCATTTCAG CCTTGGCAGA GGTGGATAAC TGCACCAGCA AGAGTAAGAA GAAGAAGAAA 
ACTGATTTTA TTCCCTACAG AGATTCTGTA CTTACTTGGC TCCTTCGAGA AAATCTAGGT 
GGCAATTCTC GGACTGCAAT GGTTGCTGCT CTGAGCCCAG CTGATATCAA CTATGATGAA 
ACTTTGAGCA CTCTGAGATA CGCAGATCGT GCAAAACAAA TTAAATGTAA TGCTGTTATC 
AATGAGGACC CCAATGCCAA ACTGGTTCGT GAATTAAAGG AGGAGGTGAC ACGGCTGAAG 
GACCTTCTTC GTGCTCAGGG GCTGGGAGAT ATTATTGATA CATCCATGGG GTCCCTTACT 
TCATCTCCAT CTTCCTGCTC ACTCAATAGT CAGGTGGGCT TAACATCTGT GACCAGTATT 
CAGGAGAGAA TCATGTCTAC ACCTGGAGGA GAGGAAGCCA TCGAACGTCT GAAGGAATCA 
GAGAAGATCA TTGCTGAGTT GAATGAAACC TGGGAAGAGA AGCTGCGTAA AACAGAGGCC 
ATCAGGATGG AGAGAGAAGC CTTATTGGCT GAGATGGGAG TTGCCATTCG GGAAGACGGA 
GGAACCCTTG GGGTTTTCTC TCCTAAAAAG ACACCACATC TTGTTAACCT CAATGAGGAC 
CCTCTAATGT CCGAGTGCCT GCTTTATTAC ATCAAAGATG GAATTACAAG GGTTGGCCAA 
GCAGATGCTG AGCGGCGCCA GGACATCGTG CTGAGTGGGG CTCACATTAA AGAAGAGCAT 
TGTATCTTCC GGAGCGAGAG GAACAACAGC GGGGATGTTA TTGTGACTCT GGAGCCCTGT 
GAACGTTCAG AAACCTACGT AAATGGCAAG AGGGTGACCC AGCCTGTTCA GCTGCGCTCA 
GGAAACCGTA TCATCATGGG TAAAAACCAC GTGTTCCGTT TTAACCACCC GGAGCAAGCA 
CGGGCTGAGC GGGAAAAGAC TCCTTCTGCT GAGACCCCCT CTGAGCCTGT GGACTGGACG 
TTTGCCCAGA GAGAGCTTCT GGAAAAACAA GGAATTGATA TGAAACAGGA GATGGAAAAA 
AGGCTACAGG AAATGGAGAT CCTATACAAA AAGGAGAAGG AAGAAGCAGA TCTTCTCTTG 
GAGCAGCAGA GACTGGACTA TGAGAGTAAA TTGCAGGCCT TGCAGAAGCA GGTGGAGACG 
CGATCCCTGG CTGCAGAAAC AACTGAAGAG GAAGAGGAGG AGGAAGAAGT TCCGTGGACA 
CAACATGAAT TTGAGCTGGC CCAGTGGGCC TTCCGGAAGT GGAAGTCTCA CCAATTCACC 
TCATTAAGGG ACTTACTCTG GGGCAATGCC GTTTACCTAA AGGAGGCCAA TGCCATCAGT 
GTGGAACTAA AGAAGAAGGT GCAGTTTCAG TTTGTTCTGC TCACGGACAC GTTGTACTCC 
CCTCTGCCTC CTGAACTGCT TCCCACTGAG ATTGAAAAAA CTCAGGAAGA CAGACCTTTC 
CCTCGTACAG TGGTAGCTGT AGAAGTTCAG GATCTGAAAA ATGGAGCAAC ACATTATTGG 
TCTTTGGAGA AACTTAAGCA GAGGCTGGAC TTGATGAGAG AGATGTATGA CAGAGCAGGG 
GAGATGGCCT CCAGCGCCCA AGATGAAAGT GAGACCACCA TGACTGGCAG CGATCCATTT 
TATGATCGGT TCCATTGGTT CAAACTTGTG GGAAGGGCCT TTGTTTACCT GAGCAACCTG 
CTGTACCCCG TGCCCCTGAT TCACAGGGTG GCCATCGTCA GTGAGAAGGG AGAGGTGCGT 
GGATTTCTGC GTGTGGCTGT GCAGGCCATT GCAGCGGATG AAGAAGCTCC TGATTATGGC 
TCTGGAATTC GACAGTCAGG AACAGCTAAA ATATCTTTTG ATAACGAGTA CTTTAATCAG 
AGTGACGTTT CTTCGGTTGC ATTGACCCGT TCTGGTCTGT CCTTGGAGGA GCTGAGGATT 
GTGGAAGGAC AGGGTCAGAG TTCTGAGGTC ATCACTCCTC CAGAAGAAGT CAACCGAATG 
AATGATCTGG ATCTGAAGTC AAGCACTTTG CTGGATGGTA AGATGATGAT GGAGGGATTT 
TCTGAAGAGA TCGGCAACCA CCTAAAGCTG GGCAGTGCAT TCACTTTCCG GGTCACAGTG 
CTGCAGGCCA GTGGAATCCT CCCAGAGTAT GCAGACATCT TCTGTCAGTT CAACTTTTTA 
CATCGGCATG ATGAAGCATT CTCCACTGAA CCCCTCAAGA ACAACGGCAG AGGAAGTCCT 
CTGGGCTTTT ACCATGTACA GAATATTGCA GTGGAGGTTA CCGAATCATT CGTGGACTAT 
ATCAAAACCA AGCCGATTGT GTTTGAAGTC TTTGGGCATT ATCAGCAGCA CCCACTTCAC 
CTGCAAGGAC AGGAACTTCA CAGTCCACCC CAGCCGTCTC GACGATTCTT CCCTCCGCCC 
ATGCCGCTAT CCAAGCCAGT TCCAGCCACC AAGTTAAATA CCATGAGCAA AACCAGCCTT 
GGCCAGAGCA TGAGCAAGTA TGATCTTCTG GTTTGGTTTG AGATCAGTGA ACTGGAGCCT 
ACGGGAGAGT ATATCCCAGC CGTGGTGGAC CATACAGCAG GCTTGCCCTG CCAGGGGACG 
TTTTTGCTTC ACCAGGGCAT CCAGCGGAGG ATCACAGTGA CCATCATCCA TGAGAAGGGG 
AGTGAGCTCC ACTGGAAGGA TGTTCGTGAG CTGGTGGTAG GCCGAATCAG GAATAAGGCT 
GAGGTGGATG AAGCTGCAGT GGACGCCATC CTCTCCCTCA ATATCATCTC TGCCAAGTAC 
CTGAAGTCGT CCCACAACTG TAGCAGGACC TTCTATCGTT TCGAGGCCGT CTGGGATAGT 
TCCCTCCATA ACTCCCTCCT TCTGAACCGA GTGACACCCT ACGGAGAAAA GATCTACATG 
ACCCTGTCGG CCTACCTGGA GCTGGATCAC TGCATCCAGC CGGCCGTCAT CACCAAGGAC 
GTGTGCATGG TCTTCTACTC CCGGGACGCC AAGATCTCGC CGCCACGCTC GCTGCGCAGC 
CTCTTTGGCA GCGGCTATTC GAAGTCACCA GACTCCAATC GAGTCACTGG AATTTATGAA 
CTCAGTTTAT GCAAAATGGC AGACACAGGA AGTCCAGGCA TGCAGCGGAG AAGAAGGAAA 
ATCTTGGATA CCTCAGTGGC GTATGTGCGG GGGGAGGAGA ACTTGGCTGG CTGGCGGCCC 
CGGGGAGACA GCCTCATCCT AGAGCACCAG TGGGAGTTGG AGAAGCTGGA GCTCCTGCAC 
GAGGTGGAGA AAACCCGACA CTTTCTGCTG CTCCGTGACA GACTGGGTGA CAGCATCCCC 
AAATCCCTGA GTGACTCGTT GTCCCCCAGC CTCAGCAGCG GGACCCTCAG CACCTCCACC 
AGCATCTCCT CTCAGATCTC AACCACCACC TTTGAAAGTG CCATCACACC CAGCGAGAGT 
AGTGGCTACG ACTCAGCAGA CATTGAAAGC CTGGTGGATC GAGAGAAAGA GCTGGCTACA 
AAGTGCCTGC AACTTCTCAC CCACACTTTC AACCGAGAAT TCAGCCAGGT GCACGGCAGC 
ATCAGTGACT GCAAGCTGGC TGACCTCTCC CCCGTCGGCC GGGACCCCTC TGTGTCCAGT 
CTGAGCAGTT CCACCCTCAC GCCATCCTCT ACCTGCCCCT CTCTGGTGGA TGCTAGGAGC 
AACTCCCTGG ATCAGAAGAC CCCAGAAGCC AATTCCCGGG CCTCTAGTCC CTGCCCAGAA 
TTTGAACAGT TTCAGATTGT CCCACCTGTG GAAACACCCT ATTTGGCCCG AGCAGGGAAA 
AATGAATTTC TCAATCTTGT TCCAGATATT GAAGAAATTA GACCAGGCTC AGTGGTCTCC 
AAGAAAGGAT ATCTGCATTT CAAGGAGCCC CTGTCCAGCA ACTGGGCTAA ACATTTCGTC 
GTGGTCCGTC GCCCTTACGT CTTCATCTAT AACAGCGACA AAGACCCTGT GGAGCGGGGC 
ATCATTAACC TGTCCACAGC CCAGGTGGAG TATAGCGAGG ACCAGCAGGC CATGGTGAAG 
ACACCGAACA CCTTTGCTGT GTGCACCAAG CACCGTGGGG TCCTTTTGCA GGCTCTCAAC 
GACAAAGACG TGAACGACTG GTTGTACGCC TTTAACCCGC TCCTTGCTGG CACAATACGG 
TCAAAACTCT CCCGCAGATG CCCGAGCCAA GCGAAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KIF1B
Entrez Gene ID	100337161
Full Name	kinesin family member 1B
General protein information	Preferred Names kinesin family member 1B Names kinesin-like protein KIF1B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	LOC722674	XP_002808317.1
Gallus gallus (chicken)	KIF1B	XP_004947511.1
Rattus norvegicus (Norway rat)	Kif1b	NP_476548.1
Canis lupus familiaris (dog)	KIF1B	XP_536743.3
Mus musculus (house mouse)	Kif1b	NP_997565.2
Danio rerio (zebrafish)	kif1b	NP_919363.2
Homo sapiens (human)	KIF1B	NP_055889.2
Bos taurus (cattle)	KIF1B	XP_005217049.1
Xenopus tropicalis (tropical clawed frog)	kif1b	XP_002939603.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KIF1B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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