MGAM cDNA ORF clone, Bos taurus(cattle)

The following MGAM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGAM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa89792 XM_015470806.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa89792 XM_015463156.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa234069 XM_024991197.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa234069 XM_024991198.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa234069 XM_015470805.2
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa234070 XM_024991199.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa234070 XM_005205921.4
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa234070 XM_024991200.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa89791 XM_015463154.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89791 XM_015470805.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa18931 XM_015463155.1
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa18931 XM_005205921.3
Latest version!
Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa89792
    Clone ID Related Accession (Same CDS sequence) XM_015470806.1 , XM_015463156.1
    Accession Version XM_015470806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAAGAAG TCGTGGAGAG AAACCGTGCT GCGCAGCTGC CTTATGATGT TCAGCATGCT 
    GATATTGATT ACATGGACGC AAGAAAGGAC TTCACTTATG ATCCAGTAGC TTTCAAGGGC
    TTCCCTGAGT TTGTTAAAGA ATTACACAAT AATGGACAGA AACTGGTCAT CATTGTGGAT
    CCTGCCATCT CCAACAACTC TTCCCTAAGT AATCCCTATG GCCCGTATGA CCGGGGTTCA
    GATATGAAGA TATGGGTGAA TACTTCAGAC GGGGTGACTC CACTCATTGG GGAGGTCTGG
    CCTGGAAAAA CTGTGTTTCC TGATTACACC AACCCTAAGT GTACTGCTTG GTGGACAAAT
    GAATTTGAGC TTTTCCACAG TCAAGTAGAG TTTGATGGAA TCTGGATTGA TATGAATGAA
    GTTGCCAACT TTGTGGATGG CTCAGTTTCA GGATGTTCCA CGAGCAATCT AAATTATCCC
    CCATTCACTC CCAAAATCCT GGATGGGTAC CTGTTCAGCA AGAGCATCTG CATGGACGCA
    GTGCAGCACT GGGGGCAGCA TTACGATGTC CACAATCTGT ACGGCTACTC CATGGCCATC
    ACCACTGCAG AAACTGTCAA GACTGTGTTC CCCAACAAGA GAAGCCTCAT TTTAACCCGT
    TCTACGTTTG CGGGTTCTGG CAAGTTTGCA GCCCACTGGC TAGGAGACAA TGCCGCCACC
    TGGAGCGACC TTCGATGGTC CATCCCTGGC ATGCTTGAGT TCAACCTGTT TGGTATCCCC
    ATGGTGGGCG CTGACATATG TGGCTTTATG CTGGATACTT CTGAAGAGCT CTGCAGACGG
    TGGATGCAGC TGGGAGCGTT CTATCCATTT TCCAGGAATC ACAATGGCCA AGGCTACAAG
    GCCCAGGATC CAGCCTCCTT TGGCCCTGAC TCACTGCTGT TGAATTCCTC CAGGCACTAC
    CTGACCATCC GCTATACCCT TCTGCCCTAT CTCTACACAC TCTTCTACCG TGCTCACAGC
    CGCGGGGACA CTGTGGCCAG GCCCCTTCTG CATGAGTTCT ACCAGGACAG CAACACCTGG
    GATGTGCACC AGCAGTTTTT ATGGGGGCCG GGCCTCCTCA TCACTCCAGT CCTGGATGAG
    GGTGCAGAGA AAGTGACCGC ATACATGCCT GACGCTGTCT GGTATGACTA TGAGACTGGG
    GGCCGAGTGA GATGGAGGAA GCAAAAAGTG GAGATGGAAC TTCCTGGAGA CAAAATTGGA
    CTTCACCTGC GAGGAGGCTA CATCTTCCCC ACGCAGCAGC CAGCCACGAC CACGGTGGCC
    AGTCGACGGA ACCCTCTCGG TCTAATCATT GCCCTGGATG AGAACAAAGA AGCAAAAGGA
    GAACTTTTCT GGGATGATGG GGAAACAAAA GATACTGTGG CCAACAACCT TTATCTCTTA
    TGTGAGTTCT CCGTCACCCA AAACCGCCTG GAGGTTAAGA TTCTGCAATC AACCTACACA
    GACCCCAATA ATTTAGCATT TAAAGAAATT AAAATCCTTG GAACCCAGGA GCCTAACAAG
    GTTACAGTGA AACAGAACGG TGTTCCAATT CAAGTTTCTC CTAATGTCAC TTACGATTCC
    AACCTACAGG TGGCCCTTAT CACAGAAATT GATCTTGTTC TGGGAGGAAC ATACACGGTG
    GAGTGGGATG TGAAGATAAG GGATGAAGAA AAAATAGACT GTTATCCTGA TGAGACTGGT
    GTTTCTGCAG AAAACTGCAC TGCCCGCGGT TGTGCCTGGG AGGAGTCCAG TTCTCATGGA
    GTCCCTTTTT GCTATTTCGT CAATGATCTC TACTCTGTCA GTGATGTTCA GTATGACTCA
    CATGGGGCCT CAGCTGTCAT CACCTTAAAG ACTTCTCTGT ATGCCCACTC CTTCCCCTCA
    GTACCAGTGA ACTCCCTGCG CCTGACTGTG ACCTACCATA AGGATAACAT GCTGCAGTTT
    AAGATTTATG ATCCCAGCAA CAACCGGTAT GAAGTTCCAG TCCCTCTCAA CATACCCAGC
    ATTCCATCTG GCACCTCTGA GAGTCAACTC TATGCTGTCC TCATTAAGAA GAATCCATTT
    GGGATTGAAA TTCGCCGCAA GAGTACAGGC ACTGTCATCT GGGACTCTCA GCTCCTCGGC
    TTTACCTTCA ATGACATGTT CATCCGCATC TCCACGCGCC TCCCCTCCAA GTACCTCTAT
    GGCTTTGGGG AAACTGAGCA CACAGCCTTC CGAAGAGACC TGGAATGGAA CACGTGGGGG
    ATGTTCTCCC GAGACCAACC CCCGGGGTAC AAGAAGAACT CCTATGGTGT CCACCCCTAC
    TACATGGCAC TGGAGGAGGA TGGCAGCGCC CACGGAGTGC TCCTGCTGAA CAGCAATGCC
    ATGGATGTGA CATTCCAGCC CTTGCCTGCC TTGACATACC GTACCACAGG AGGAATTCTG
    GACTTTTATG TGGTTTTAGG GCCAACTCCA GAGCTTGTCA CTCAGCAGTA CACTGAGTTG
    ATCGGTCGGC CAGTGATGGT TCCTTACTGG TCCCTGGGGT TTCAGCTGTG TCGCTATGGA
    TACCAGAACG ACTCTGAGAT CGCCAGCTTG TACGATGCTA TGGTGGCTGC ACAGATCCCC
    TATGTATGTC GTCAGCCTGT GGAGTTCTGG CTCTGGGACC TCATGGATGT GCAGTATTCG
    GACATTGACT ACATGGAGCG GCAGCTGGAC TTCACCCTTG ATGCCGAGTT TGAGGGGTTT
    CCAGCTCTGA TTACTCGCAT GAGGGCCGAT GGGATGCGAG TCATTATCAT TCTGGACCCA
    GCCATTTCTG GCAACGAGAC CAAGCCCTAT CTGCCATTCA CACGGGGTGT AGAGGATGAT
    GTCTTCATCA AAGATCCGAG TGATGGGAGC ATTGTCTGGG GAAAGGTCTG GCCTGATTTT
    CCTGATGTGG TAATAAACAG TTCTCTAGAC TGGGACAGTC AAGTGGAGAA ATACCGGGCA
    TTTGTGGCCT TTCCAGACTT TTTCCGTAAT TCAACCACCA CATGGTGGAA GAGGGAGTTG
    AGAGAACTGT ACACCAATCC ACGGGAACCA GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG
    ATTGATATGA ATGAACCAGC GAGCTTCGTG AATGGAGCAG TCCCTCCAGG CTGCAAGGAT
    GCCACTCTGA ACCACCCTCC CTATATGCCA TATCTGGAGT CCAGGGACCG GGGCCTGAGC
    AGCAAGACCC TGTGCATGGA GAGCCAGCAG GTCCTGCCTG ACGGCTCACC GGTGCGCCAC
    TACGACGTGC ACAGCCTCTA CGGGTGGGCC CAGACCAGAC CCACATACGA AGCTGTGCAG
    GAGGTGACGG GACAGCGAGG GATCGTCATC ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC
    TGGGGAGGAC ACTGGCTGGG TGACAACACG GCTGCATGGG ACCAGCTGAA GAAGTCAATC
    ATTGGCATGA TGGAGTTTAG TCTCTTTGGT ATATCCTACA CAGGAGCAGA TATTTGTGGA
    TTCTTTCAAG ATGCTGAATA TGAAATGTGT GCTCGCTGGA TGCAACTAGG GGCCTTCTAC
    CCCTTCGCAA GGAATCACAA TACCATTGGG ACCAAGAGGC AAGACCCCGT GTCCTGGAAT
    TCTACTTTCG TGACAATCTC CAAGAGTGTC CTGGAGACCA GATACACCCT GTTGCCGTAT
    ATCTACACCC TGATGCACAA GGCCAGCACA GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC
    CATGAGTTTG TGTCAGACCG GGTGACCTGG GATGTAGACA GTCAGTTCCT GCTGGGCCCA
    GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT AACGCCAGAG ATGTCACTGC ATATTTCCCT
    AGAGCCCGCT GGTATGATTA CTACACGGGT GTGGACATTC AATCCCGGGG TGAGTGGAAG
    TCCTTGCCGG CACCTCTTGA CCACATTAAT CTTCATGTCC GTGGGGGCTA TGTCCTGCCC
    TGGCAAGAGC CTGCACAGAA CACCCACTTA AGCCGTCAGA AATTCTTAGG TTTCAAGGTT
    GCCTTGGATG ATGAGGGGGC TGCTGAAGGC TGGCTCTTCT GGGATGATGG GCAAAGCATT
    GATACCTATG AAAAAGGACT CTATTACCTG GCGCACTTTT CCGTCAGCCA GAATATGATG
    CACAGTCATA TAATTTTCAA CAACTACATC AGCAATACAA ACCCTTTGAG ACTGGGCTAC
    TTTGAGGTCT GGGGAGTGGG CAGTGACTCC ATTTCCAGTG TCAGCATCTC TGCAAGTGGC
    GTGGTTATAA CACCCACCTT CTCCTACAAC TCTACAACAC AGGTGTTAAA TATTGATGTG
    ACCAGCAGAA ACATCAGCCT ACATAATTTC ACTTCATTGA TGTGGAGAAG CACGCCGAAT
    TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326292.1
    CDS351..4796
    Translation

    Target ORF information:

    RefSeq Version XM_015470806.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAAGAAG TCGTGGAGAG AAACCGTGCT GCGCAGCTGC CTTATGATGT TCAGCATGCT 
    GATATTGATT ACATGGACGC AAGAAAGGAC TTCACTTATG ATCCAGTAGC TTTCAAGGGC
    TTCCCTGAGT TTGTTAAAGA ATTACACAAT AATGGACAGA AACTGGTCAT CATTGTGGAT
    CCTGCCATCT CCAACAACTC TTCCCTAAGT AATCCCTATG GCCCGTATGA CCGGGGTTCA
    GATATGAAGA TATGGGTGAA TACTTCAGAC GGGGTGACTC CACTCATTGG GGAGGTCTGG
    CCTGGAAAAA CTGTGTTTCC TGATTACACC AACCCTAAGT GTACTGCTTG GTGGACAAAT
    GAATTTGAGC TTTTCCACAG TCAAGTAGAG TTTGATGGAA TCTGGATTGA TATGAATGAA
    GTTGCCAACT TTGTGGATGG CTCAGTTTCA GGATGTTCCA CGAGCAATCT AAATTATCCC
    CCATTCACTC CCAAAATCCT GGATGGGTAC CTGTTCAGCA AGAGCATCTG CATGGACGCA
    GTGCAGCACT GGGGGCAGCA TTACGATGTC CACAATCTGT ACGGCTACTC CATGGCCATC
    ACCACTGCAG AAACTGTCAA GACTGTGTTC CCCAACAAGA GAAGCCTCAT TTTAACCCGT
    TCTACGTTTG CGGGTTCTGG CAAGTTTGCA GCCCACTGGC TAGGAGACAA TGCCGCCACC
    TGGAGCGACC TTCGATGGTC CATCCCTGGC ATGCTTGAGT TCAACCTGTT TGGTATCCCC
    ATGGTGGGCG CTGACATATG TGGCTTTATG CTGGATACTT CTGAAGAGCT CTGCAGACGG
    TGGATGCAGC TGGGAGCGTT CTATCCATTT TCCAGGAATC ACAATGGCCA AGGCTACAAG
    GCCCAGGATC CAGCCTCCTT TGGCCCTGAC TCACTGCTGT TGAATTCCTC CAGGCACTAC
    CTGACCATCC GCTATACCCT TCTGCCCTAT CTCTACACAC TCTTCTACCG TGCTCACAGC
    CGCGGGGACA CTGTGGCCAG GCCCCTTCTG CATGAGTTCT ACCAGGACAG CAACACCTGG
    GATGTGCACC AGCAGTTTTT ATGGGGGCCG GGCCTCCTCA TCACTCCAGT CCTGGATGAG
    GGTGCAGAGA AAGTGACCGC ATACATGCCT GACGCTGTCT GGTATGACTA TGAGACTGGG
    GGCCGAGTGA GATGGAGGAA GCAAAAAGTG GAGATGGAAC TTCCTGGAGA CAAAATTGGA
    CTTCACCTGC GAGGAGGCTA CATCTTCCCC ACGCAGCAGC CAGCCACGAC CACGGTGGCC
    AGTCGACGGA ACCCTCTCGG TCTAATCATT GCCCTGGATG AGAACAAAGA AGCAAAAGGA
    GAACTTTTCT GGGATGATGG GGAAACAAAA GATACTGTGG CCAACAACCT TTATCTCTTA
    TGTGAGTTCT CCGTCACCCA AAACCGCCTG GAGGTTAAGA TTCTGCAATC AACCTACACA
    GACCCCAATA ATTTAGCATT TAAAGAAATT AAAATCCTTG GAACCCAGGA GCCTAACAAG
    GTTACAGTGA AACAGAACGG TGTTCCAATT CAAGTTTCTC CTAATGTCAC TTACGATTCC
    AACCTACAGG TGGCCCTTAT CACAGAAATT GATCTTGTTC TGGGAGGAAC ATACACGGTG
    GAGTGGGATG TGAAGATAAG GGATGAAGAA AAAATAGACT GTTATCCTGA TGAGACTGGT
    GTTTCTGCAG AAAACTGCAC TGCCCGCGGT TGTGCCTGGG AGGAGTCCAG TTCTCATGGA
    GTCCCTTTTT GCTATTTCGT CAATGATCTC TACTCTGTCA GTGATGTTCA GTATGACTCA
    CATGGGGCCT CAGCTGTCAT CACCTTAAAG ACTTCTCTGT ATGCCCACTC CTTCCCCTCA
    GTACCAGTGA ACTCCCTGCG CCTGACTGTG ACCTACCATA AGGATAACAT GCTGCAGTTT
    AAGATTTATG ATCCCAGCAA CAACCGGTAT GAAGTTCCAG TCCCTCTCAA CATACCCAGC
    ATTCCATCTG GCACCTCTGA GAGTCAACTC TATGCTGTCC TCATTAAGAA GAATCCATTT
    GGGATTGAAA TTCGCCGCAA GAGTACAGGC ACTGTCATCT GGGACTCTCA GCTCCTCGGC
    TTTACCTTCA ATGACATGTT CATCCGCATC TCCACGCGCC TCCCCTCCAA GTACCTCTAT
    GGCTTTGGGG AAACTGAGCA CACAGCCTTC CGAAGAGACC TGGAATGGAA CACGTGGGGG
    ATGTTCTCCC GAGACCAACC CCCGGGGTAC AAGAAGAACT CCTATGGTGT CCACCCCTAC
    TACATGGCAC TGGAGGAGGA TGGCAGCGCC CACGGAGTGC TCCTGCTGAA CAGCAATGCC
    ATGGATGTGA CATTCCAGCC CTTGCCTGCC TTGACATACC GTACCACAGG AGGAATTCTG
    GACTTTTATG TGGTTTTAGG GCCAACTCCA GAGCTTGTCA CTCAGCAGTA CACTGAGTTG
    ATCGGTCGGC CAGTGATGGT TCCTTACTGG TCCCTGGGGT TTCAGCTGTG TCGCTATGGA
    TACCAGAACG ACTCTGAGAT CGCCAGCTTG TACGATGCTA TGGTGGCTGC ACAGATCCCC
    TATGTATGTC GTCAGCCTGT GGAGTTCTGG CTCTGGGACC TCATGGATGT GCAGTATTCG
    GACATTGACT ACATGGAGCG GCAGCTGGAC TTCACCCTTG ATGCCGAGTT TGAGGGGTTT
    CCAGCTCTGA TTACTCGCAT GAGGGCCGAT GGGATGCGAG TCATTATCAT TCTGGACCCA
    GCCATTTCTG GCAACGAGAC CAAGCCCTAT CTGCCATTCA CACGGGGTGT AGAGGATGAT
    GTCTTCATCA AAGATCCGAG TGATGGGAGC ATTGTCTGGG GAAAGGTCTG GCCTGATTTT
    CCTGATGTGG TAATAAACAG TTCTCTAGAC TGGGACAGTC AAGTGGAGAA ATACCGGGCA
    TTTGTGGCCT TTCCAGACTT TTTCCGTAAT TCAACCACCA CATGGTGGAA GAGGGAGTTG
    AGAGAACTGT ACACCAATCC ACGGGAACCA GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG
    ATTGATATGA ATGAACCAGC GAGCTTCGTG AATGGAGCAG TCCCTCCAGG CTGCAAGGAT
    GCCACTCTGA ACCACCCTCC CTATATGCCA TATCTGGAGT CCAGGGACCG GGGCCTGAGC
    AGCAAGACCC TGTGCATGGA GAGCCAGCAG GTCCTGCCTG ACGGCTCACC GGTGCGCCAC
    TACGACGTGC ACAGCCTCTA CGGGTGGGCC CAGACCAGAC CCACATACGA AGCTGTGCAG
    GAGGTGACGG GACAGCGAGG GATCGTCATC ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC
    TGGGGAGGAC ACTGGCTGGG TGACAACACG GCTGCATGGG ACCAGCTGAA GAAGTCAATC
    ATTGGCATGA TGGAGTTTAG TCTCTTTGGT ATATCCTACA CAGGAGCAGA TATTTGTGGA
    TTCTTTCAAG ATGCTGAATA TGAAATGTGT GCTCGCTGGA TGCAACTAGG GGCCTTCTAC
    CCCTTCGCAA GGAATCACAA TACCATTGGG ACCAAGAGGC AAGACCCCGT GTCCTGGAAT
    TCTACTTTCG TGACAATCTC CAAGAGTGTC CTGGAGACCA GATACACCCT GTTGCCGTAT
    ATCTACACCC TGATGCACAA GGCCAGCACA GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC
    CATGAGTTTG TGTCAGACCG GGTGACCTGG GATGTAGACA GTCAGTTCCT GCTGGGCCCA
    GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT AACGCCAGAG ATGTCACTGC ATATTTCCCT
    AGAGCCCGCT GGTATGATTA CTACACGGGT GTGGACATTC AATCCCGGGG TGAGTGGAAG
    TCCTTGCCGG CACCTCTTGA CCACATTAAT CTTCATGTCC GTGGGGGCTA TGTCCTGCCC
    TGGCAAGAGC CTGCACAGAA CACCCACTTA AGCCGTCAGA AATTCTTAGG TTTCAAGGTT
    GCCTTGGATG ATGAGGGGGC TGCTGAAGGC TGGCTCTTCT GGGATGATGG GCAAAGCATT
    GATACCTATG AAAAAGGACT CTATTACCTG GCGCACTTTT CCGTCAGCCA GAATATGATG
    CACAGTCATA TAATTTTCAA CAACTACATC AGCAATACAA ACCCTTTGAG ACTGGGCTAC
    TTTGAGGTCT GGGGAGTGGG CAGTGACTCC ATTTCCAGTG TCAGCATCTC TGCAAGTGGC
    GTGGTTATAA CACCCACCTT CTCCTACAAC TCTACAACAC AGGTGTTAAA TATTGATGTG
    ACCAGCAGAA ACATCAGCCT ACATAATTTC ACTTCATTGA TGTGGAGAAG CACGCCGAAT
    TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89792
    Clone ID Related Accession (Same CDS sequence) XM_015470806.1 , XM_015463156.1
    Accession Version XM_015463156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAAGAAG TCGTGGAGAG AAACCGTGCT GCGCAGCTGC CTTATGATGT TCAGCATGCT 
    GATATTGATT ACATGGACGC AAGAAAGGAC TTCACTTATG ATCCAGTAGC TTTCAAGGGC
    TTCCCTGAGT TTGTTAAAGA ATTACACAAT AATGGACAGA AACTGGTCAT CATTGTGGAT
    CCTGCCATCT CCAACAACTC TTCCCTAAGT AATCCCTATG GCCCGTATGA CCGGGGTTCA
    GATATGAAGA TATGGGTGAA TACTTCAGAC GGGGTGACTC CACTCATTGG GGAGGTCTGG
    CCTGGAAAAA CTGTGTTTCC TGATTACACC AACCCTAAGT GTACTGCTTG GTGGACAAAT
    GAATTTGAGC TTTTCCACAG TCAAGTAGAG TTTGATGGAA TCTGGATTGA TATGAATGAA
    GTTGCCAACT TTGTGGATGG CTCAGTTTCA GGATGTTCCA CGAGCAATCT AAATTATCCC
    CCATTCACTC CCAAAATCCT GGATGGGTAC CTGTTCAGCA AGAGCATCTG CATGGACGCA
    GTGCAGCACT GGGGGCAGCA TTACGATGTC CACAATCTGT ACGGCTACTC CATGGCCATC
    ACCACTGCAG AAACTGTCAA GACTGTGTTC CCCAACAAGA GAAGCCTCAT TTTAACCCGT
    TCTACGTTTG CGGGTTCTGG CAAGTTTGCA GCCCACTGGC TAGGAGACAA TGCCGCCACC
    TGGAGCGACC TTCGATGGTC CATCCCTGGC ATGCTTGAGT TCAACCTGTT TGGTATCCCC
    ATGGTGGGCG CTGACATATG TGGCTTTATG CTGGATACTT CTGAAGAGCT CTGCAGACGG
    TGGATGCAGC TGGGAGCGTT CTATCCATTT TCCAGGAATC ACAATGGCCA AGGCTACAAG
    GCCCAGGATC CAGCCTCCTT TGGCCCTGAC TCACTGCTGT TGAATTCCTC CAGGCACTAC
    CTGACCATCC GCTATACCCT TCTGCCCTAT CTCTACACAC TCTTCTACCG TGCTCACAGC
    CGCGGGGACA CTGTGGCCAG GCCCCTTCTG CATGAGTTCT ACCAGGACAG CAACACCTGG
    GATGTGCACC AGCAGTTTTT ATGGGGGCCG GGCCTCCTCA TCACTCCAGT CCTGGATGAG
    GGTGCAGAGA AAGTGACCGC ATACATGCCT GACGCTGTCT GGTATGACTA TGAGACTGGG
    GGCCGAGTGA GATGGAGGAA GCAAAAAGTG GAGATGGAAC TTCCTGGAGA CAAAATTGGA
    CTTCACCTGC GAGGAGGCTA CATCTTCCCC ACGCAGCAGC CAGCCACGAC CACGGTGGCC
    AGTCGACGGA ACCCTCTCGG TCTAATCATT GCCCTGGATG AGAACAAAGA AGCAAAAGGA
    GAACTTTTCT GGGATGATGG GGAAACAAAA GATACTGTGG CCAACAACCT TTATCTCTTA
    TGTGAGTTCT CCGTCACCCA AAACCGCCTG GAGGTTAAGA TTCTGCAATC AACCTACACA
    GACCCCAATA ATTTAGCATT TAAAGAAATT AAAATCCTTG GAACCCAGGA GCCTAACAAG
    GTTACAGTGA AACAGAACGG TGTTCCAATT CAAGTTTCTC CTAATGTCAC TTACGATTCC
    AACCTACAGG TGGCCCTTAT CACAGAAATT GATCTTGTTC TGGGAGGAAC ATACACGGTG
    GAGTGGGATG TGAAGATAAG GGATGAAGAA AAAATAGACT GTTATCCTGA TGAGACTGGT
    GTTTCTGCAG AAAACTGCAC TGCCCGCGGT TGTGCCTGGG AGGAGTCCAG TTCTCATGGA
    GTCCCTTTTT GCTATTTCGT CAATGATCTC TACTCTGTCA GTGATGTTCA GTATGACTCA
    CATGGGGCCT CAGCTGTCAT CACCTTAAAG ACTTCTCTGT ATGCCCACTC CTTCCCCTCA
    GTACCAGTGA ACTCCCTGCG CCTGACTGTG ACCTACCATA AGGATAACAT GCTGCAGTTT
    AAGATTTATG ATCCCAGCAA CAACCGGTAT GAAGTTCCAG TCCCTCTCAA CATACCCAGC
    ATTCCATCTG GCACCTCTGA GAGTCAACTC TATGCTGTCC TCATTAAGAA GAATCCATTT
    GGGATTGAAA TTCGCCGCAA GAGTACAGGC ACTGTCATCT GGGACTCTCA GCTCCTCGGC
    TTTACCTTCA ATGACATGTT CATCCGCATC TCCACGCGCC TCCCCTCCAA GTACCTCTAT
    GGCTTTGGGG AAACTGAGCA CACAGCCTTC CGAAGAGACC TGGAATGGAA CACGTGGGGG
    ATGTTCTCCC GAGACCAACC CCCGGGGTAC AAGAAGAACT CCTATGGTGT CCACCCCTAC
    TACATGGCAC TGGAGGAGGA TGGCAGCGCC CACGGAGTGC TCCTGCTGAA CAGCAATGCC
    ATGGATGTGA CATTCCAGCC CTTGCCTGCC TTGACATACC GTACCACAGG AGGAATTCTG
    GACTTTTATG TGGTTTTAGG GCCAACTCCA GAGCTTGTCA CTCAGCAGTA CACTGAGTTG
    ATCGGTCGGC CAGTGATGGT TCCTTACTGG TCCCTGGGGT TTCAGCTGTG TCGCTATGGA
    TACCAGAACG ACTCTGAGAT CGCCAGCTTG TACGATGCTA TGGTGGCTGC ACAGATCCCC
    TATGTATGTC GTCAGCCTGT GGAGTTCTGG CTCTGGGACC TCATGGATGT GCAGTATTCG
    GACATTGACT ACATGGAGCG GCAGCTGGAC TTCACCCTTG ATGCCGAGTT TGAGGGGTTT
    CCAGCTCTGA TTACTCGCAT GAGGGCCGAT GGGATGCGAG TCATTATCAT TCTGGACCCA
    GCCATTTCTG GCAACGAGAC CAAGCCCTAT CTGCCATTCA CACGGGGTGT AGAGGATGAT
    GTCTTCATCA AAGATCCGAG TGATGGGAGC ATTGTCTGGG GAAAGGTCTG GCCTGATTTT
    CCTGATGTGG TAATAAACAG TTCTCTAGAC TGGGACAGTC AAGTGGAGAA ATACCGGGCA
    TTTGTGGCCT TTCCAGACTT TTTCCGTAAT TCAACCACCA CATGGTGGAA GAGGGAGTTG
    AGAGAACTGT ACACCAATCC ACGGGAACCA GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG
    ATTGATATGA ATGAACCAGC GAGCTTCGTG AATGGAGCAG TCCCTCCAGG CTGCAAGGAT
    GCCACTCTGA ACCACCCTCC CTATATGCCA TATCTGGAGT CCAGGGACCG GGGCCTGAGC
    AGCAAGACCC TGTGCATGGA GAGCCAGCAG GTCCTGCCTG ACGGCTCACC GGTGCGCCAC
    TACGACGTGC ACAGCCTCTA CGGGTGGGCC CAGACCAGAC CCACATACGA AGCTGTGCAG
    GAGGTGACGG GACAGCGAGG GATCGTCATC ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC
    TGGGGAGGAC ACTGGCTGGG TGACAACACG GCTGCATGGG ACCAGCTGAA GAAGTCAATC
    ATTGGCATGA TGGAGTTTAG TCTCTTTGGT ATATCCTACA CAGGAGCAGA TATTTGTGGA
    TTCTTTCAAG ATGCTGAATA TGAAATGTGT GCTCGCTGGA TGCAACTAGG GGCCTTCTAC
    CCCTTCGCAA GGAATCACAA TACCATTGGG ACCAAGAGGC AAGACCCCGT GTCCTGGAAT
    TCTACTTTCG TGACAATCTC CAAGAGTGTC CTGGAGACCA GATACACCCT GTTGCCGTAT
    ATCTACACCC TGATGCACAA GGCCAGCACA GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC
    CATGAGTTTG TGTCAGACCG GGTGACCTGG GATGTAGACA GTCAGTTCCT GCTGGGCCCA
    GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT AACGCCAGAG ATGTCACTGC ATATTTCCCT
    AGAGCCCGCT GGTATGATTA CTACACGGGT GTGGACATTC AATCCCGGGG TGAGTGGAAG
    TCCTTGCCGG CACCTCTTGA CCACATTAAT CTTCATGTCC GTGGGGGCTA TGTCCTGCCC
    TGGCAAGAGC CTGCACAGAA CACCCACTTA AGCCGTCAGA AATTCTTAGG TTTCAAGGTT
    GCCTTGGATG ATGAGGGGGC TGCTGAAGGC TGGCTCTTCT GGGATGATGG GCAAAGCATT
    GATACCTATG AAAAAGGACT CTATTACCTG GCGCACTTTT CCGTCAGCCA GAATATGATG
    CACAGTCATA TAATTTTCAA CAACTACATC AGCAATACAA ACCCTTTGAG ACTGGGCTAC
    TTTGAGGTCT GGGGAGTGGG CAGTGACTCC ATTTCCAGTG TCAGCATCTC TGCAAGTGGC
    GTGGTTATAA CACCCACCTT CTCCTACAAC TCTACAACAC AGGTGTTAAA TATTGATGTG
    ACCAGCAGAA ACATCAGCCT ACATAATTTC ACTTCATTGA TGTGGAGAAG CACGCCGAAT
    TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318642.1
    CDS351..4796
    Translation

    Target ORF information:

    RefSeq Version XM_015463156.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAAGAAG TCGTGGAGAG AAACCGTGCT GCGCAGCTGC CTTATGATGT TCAGCATGCT 
    GATATTGATT ACATGGACGC AAGAAAGGAC TTCACTTATG ATCCAGTAGC TTTCAAGGGC
    TTCCCTGAGT TTGTTAAAGA ATTACACAAT AATGGACAGA AACTGGTCAT CATTGTGGAT
    CCTGCCATCT CCAACAACTC TTCCCTAAGT AATCCCTATG GCCCGTATGA CCGGGGTTCA
    GATATGAAGA TATGGGTGAA TACTTCAGAC GGGGTGACTC CACTCATTGG GGAGGTCTGG
    CCTGGAAAAA CTGTGTTTCC TGATTACACC AACCCTAAGT GTACTGCTTG GTGGACAAAT
    GAATTTGAGC TTTTCCACAG TCAAGTAGAG TTTGATGGAA TCTGGATTGA TATGAATGAA
    GTTGCCAACT TTGTGGATGG CTCAGTTTCA GGATGTTCCA CGAGCAATCT AAATTATCCC
    CCATTCACTC CCAAAATCCT GGATGGGTAC CTGTTCAGCA AGAGCATCTG CATGGACGCA
    GTGCAGCACT GGGGGCAGCA TTACGATGTC CACAATCTGT ACGGCTACTC CATGGCCATC
    ACCACTGCAG AAACTGTCAA GACTGTGTTC CCCAACAAGA GAAGCCTCAT TTTAACCCGT
    TCTACGTTTG CGGGTTCTGG CAAGTTTGCA GCCCACTGGC TAGGAGACAA TGCCGCCACC
    TGGAGCGACC TTCGATGGTC CATCCCTGGC ATGCTTGAGT TCAACCTGTT TGGTATCCCC
    ATGGTGGGCG CTGACATATG TGGCTTTATG CTGGATACTT CTGAAGAGCT CTGCAGACGG
    TGGATGCAGC TGGGAGCGTT CTATCCATTT TCCAGGAATC ACAATGGCCA AGGCTACAAG
    GCCCAGGATC CAGCCTCCTT TGGCCCTGAC TCACTGCTGT TGAATTCCTC CAGGCACTAC
    CTGACCATCC GCTATACCCT TCTGCCCTAT CTCTACACAC TCTTCTACCG TGCTCACAGC
    CGCGGGGACA CTGTGGCCAG GCCCCTTCTG CATGAGTTCT ACCAGGACAG CAACACCTGG
    GATGTGCACC AGCAGTTTTT ATGGGGGCCG GGCCTCCTCA TCACTCCAGT CCTGGATGAG
    GGTGCAGAGA AAGTGACCGC ATACATGCCT GACGCTGTCT GGTATGACTA TGAGACTGGG
    GGCCGAGTGA GATGGAGGAA GCAAAAAGTG GAGATGGAAC TTCCTGGAGA CAAAATTGGA
    CTTCACCTGC GAGGAGGCTA CATCTTCCCC ACGCAGCAGC CAGCCACGAC CACGGTGGCC
    AGTCGACGGA ACCCTCTCGG TCTAATCATT GCCCTGGATG AGAACAAAGA AGCAAAAGGA
    GAACTTTTCT GGGATGATGG GGAAACAAAA GATACTGTGG CCAACAACCT TTATCTCTTA
    TGTGAGTTCT CCGTCACCCA AAACCGCCTG GAGGTTAAGA TTCTGCAATC AACCTACACA
    GACCCCAATA ATTTAGCATT TAAAGAAATT AAAATCCTTG GAACCCAGGA GCCTAACAAG
    GTTACAGTGA AACAGAACGG TGTTCCAATT CAAGTTTCTC CTAATGTCAC TTACGATTCC
    AACCTACAGG TGGCCCTTAT CACAGAAATT GATCTTGTTC TGGGAGGAAC ATACACGGTG
    GAGTGGGATG TGAAGATAAG GGATGAAGAA AAAATAGACT GTTATCCTGA TGAGACTGGT
    GTTTCTGCAG AAAACTGCAC TGCCCGCGGT TGTGCCTGGG AGGAGTCCAG TTCTCATGGA
    GTCCCTTTTT GCTATTTCGT CAATGATCTC TACTCTGTCA GTGATGTTCA GTATGACTCA
    CATGGGGCCT CAGCTGTCAT CACCTTAAAG ACTTCTCTGT ATGCCCACTC CTTCCCCTCA
    GTACCAGTGA ACTCCCTGCG CCTGACTGTG ACCTACCATA AGGATAACAT GCTGCAGTTT
    AAGATTTATG ATCCCAGCAA CAACCGGTAT GAAGTTCCAG TCCCTCTCAA CATACCCAGC
    ATTCCATCTG GCACCTCTGA GAGTCAACTC TATGCTGTCC TCATTAAGAA GAATCCATTT
    GGGATTGAAA TTCGCCGCAA GAGTACAGGC ACTGTCATCT GGGACTCTCA GCTCCTCGGC
    TTTACCTTCA ATGACATGTT CATCCGCATC TCCACGCGCC TCCCCTCCAA GTACCTCTAT
    GGCTTTGGGG AAACTGAGCA CACAGCCTTC CGAAGAGACC TGGAATGGAA CACGTGGGGG
    ATGTTCTCCC GAGACCAACC CCCGGGGTAC AAGAAGAACT CCTATGGTGT CCACCCCTAC
    TACATGGCAC TGGAGGAGGA TGGCAGCGCC CACGGAGTGC TCCTGCTGAA CAGCAATGCC
    ATGGATGTGA CATTCCAGCC CTTGCCTGCC TTGACATACC GTACCACAGG AGGAATTCTG
    GACTTTTATG TGGTTTTAGG GCCAACTCCA GAGCTTGTCA CTCAGCAGTA CACTGAGTTG
    ATCGGTCGGC CAGTGATGGT TCCTTACTGG TCCCTGGGGT TTCAGCTGTG TCGCTATGGA
    TACCAGAACG ACTCTGAGAT CGCCAGCTTG TACGATGCTA TGGTGGCTGC ACAGATCCCC
    TATGTATGTC GTCAGCCTGT GGAGTTCTGG CTCTGGGACC TCATGGATGT GCAGTATTCG
    GACATTGACT ACATGGAGCG GCAGCTGGAC TTCACCCTTG ATGCCGAGTT TGAGGGGTTT
    CCAGCTCTGA TTACTCGCAT GAGGGCCGAT GGGATGCGAG TCATTATCAT TCTGGACCCA
    GCCATTTCTG GCAACGAGAC CAAGCCCTAT CTGCCATTCA CACGGGGTGT AGAGGATGAT
    GTCTTCATCA AAGATCCGAG TGATGGGAGC ATTGTCTGGG GAAAGGTCTG GCCTGATTTT
    CCTGATGTGG TAATAAACAG TTCTCTAGAC TGGGACAGTC AAGTGGAGAA ATACCGGGCA
    TTTGTGGCCT TTCCAGACTT TTTCCGTAAT TCAACCACCA CATGGTGGAA GAGGGAGTTG
    AGAGAACTGT ACACCAATCC ACGGGAACCA GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG
    ATTGATATGA ATGAACCAGC GAGCTTCGTG AATGGAGCAG TCCCTCCAGG CTGCAAGGAT
    GCCACTCTGA ACCACCCTCC CTATATGCCA TATCTGGAGT CCAGGGACCG GGGCCTGAGC
    AGCAAGACCC TGTGCATGGA GAGCCAGCAG GTCCTGCCTG ACGGCTCACC GGTGCGCCAC
    TACGACGTGC ACAGCCTCTA CGGGTGGGCC CAGACCAGAC CCACATACGA AGCTGTGCAG
    GAGGTGACGG GACAGCGAGG GATCGTCATC ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC
    TGGGGAGGAC ACTGGCTGGG TGACAACACG GCTGCATGGG ACCAGCTGAA GAAGTCAATC
    ATTGGCATGA TGGAGTTTAG TCTCTTTGGT ATATCCTACA CAGGAGCAGA TATTTGTGGA
    TTCTTTCAAG ATGCTGAATA TGAAATGTGT GCTCGCTGGA TGCAACTAGG GGCCTTCTAC
    CCCTTCGCAA GGAATCACAA TACCATTGGG ACCAAGAGGC AAGACCCCGT GTCCTGGAAT
    TCTACTTTCG TGACAATCTC CAAGAGTGTC CTGGAGACCA GATACACCCT GTTGCCGTAT
    ATCTACACCC TGATGCACAA GGCCAGCACA GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC
    CATGAGTTTG TGTCAGACCG GGTGACCTGG GATGTAGACA GTCAGTTCCT GCTGGGCCCA
    GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT AACGCCAGAG ATGTCACTGC ATATTTCCCT
    AGAGCCCGCT GGTATGATTA CTACACGGGT GTGGACATTC AATCCCGGGG TGAGTGGAAG
    TCCTTGCCGG CACCTCTTGA CCACATTAAT CTTCATGTCC GTGGGGGCTA TGTCCTGCCC
    TGGCAAGAGC CTGCACAGAA CACCCACTTA AGCCGTCAGA AATTCTTAGG TTTCAAGGTT
    GCCTTGGATG ATGAGGGGGC TGCTGAAGGC TGGCTCTTCT GGGATGATGG GCAAAGCATT
    GATACCTATG AAAAAGGACT CTATTACCTG GCGCACTTTT CCGTCAGCCA GAATATGATG
    CACAGTCATA TAATTTTCAA CAACTACATC AGCAATACAA ACCCTTTGAG ACTGGGCTAC
    TTTGAGGTCT GGGGAGTGGG CAGTGACTCC ATTTCCAGTG TCAGCATCTC TGCAAGTGGC
    GTGGTTATAA CACCCACCTT CTCCTACAAC TCTACAACAC AGGTGTTAAA TATTGATGTG
    ACCAGCAGAA ACATCAGCCT ACATAATTTC ACTTCATTGA TGTGGAGAAG CACGCCGAAT
    TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234069
    Clone ID Related Accession (Same CDS sequence) XM_015470805.2 , XM_024991197.1 , XM_024991198.1
    Accession Version XM_015470805.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326291.1
    CDS171..5708
    Translation

    Target ORF information:

    RefSeq Version XM_015470805.2
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470805.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234069
    Clone ID Related Accession (Same CDS sequence) XM_015470805.2 , XM_024991197.1 , XM_024991198.1
    Accession Version XM_024991197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846965.1
    CDS255..5792
    Translation

    Target ORF information:

    RefSeq Version XM_024991197.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234069
    Clone ID Related Accession (Same CDS sequence) XM_015470805.2 , XM_024991197.1 , XM_024991198.1
    Accession Version XM_024991198.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846966.1
    CDS59..5596
    Translation

    Target ORF information:

    RefSeq Version XM_024991198.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234070
    Clone ID Related Accession (Same CDS sequence) XM_024991200.1 , XM_005205921.4 , XM_024991199.1
    Accession Version XM_024991200.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846968.1
    CDS92..5587
    Translation

    Target ORF information:

    RefSeq Version XM_024991200.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991200.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234070
    Clone ID Related Accession (Same CDS sequence) XM_024991200.1 , XM_005205921.4 , XM_024991199.1
    Accession Version XM_005205921.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205921.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205978.1
    CDS169..5664
    Translation

    Target ORF information:

    RefSeq Version XM_005205921.4
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205921.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234070
    Clone ID Related Accession (Same CDS sequence) XM_024991200.1 , XM_005205921.4 , XM_024991199.1
    Accession Version XM_024991199.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846967.1
    CDS254..5749
    Translation

    Target ORF information:

    RefSeq Version XM_024991199.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991199.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89791
    Clone ID Related Accession (Same CDS sequence) XM_015463154.1 , XM_015470805.1
    Accession Version XM_015463154.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318640.1
    CDS211..5748
    Translation

    Target ORF information:

    RefSeq Version XM_015463154.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89791
    Clone ID Related Accession (Same CDS sequence) XM_015463154.1 , XM_015470805.1
    Accession Version XM_015470805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326291.1
    CDS211..5748
    Translation

    Target ORF information:

    RefSeq Version XM_015470805.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT
    GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG
    TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC
    ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT
    GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC
    TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG
    AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG
    AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC
    GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA
    GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC
    CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA
    CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC
    GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC
    TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG
    AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA
    GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA
    GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC
    GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC
    CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG
    CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC
    CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT
    GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG
    GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC
    TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA
    GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT
    GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC
    CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG
    AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC
    TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA
    AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA
    AGCACGCCGA ATTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18931
    Clone ID Related Accession (Same CDS sequence) XM_015463155.1 , XM_005205921.3
    Accession Version XM_015463155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318641.1
    CDS209..5704
    Translation

    Target ORF information:

    RefSeq Version XM_015463155.1
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa18931
    Clone ID Related Accession (Same CDS sequence) XM_015463155.1 , XM_005205921.3
    Accession Version XM_005205921.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product maltase-glucoamylase, intestinal isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005205978.1
    CDS209..5704
    Translation

    Target ORF information:

    RefSeq Version XM_005205921.3
    Organism Bos taurus(cattle)
    Definition Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005205921.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
    CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA
    TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT
    GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA
    ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG
    CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA
    AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC
    AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT
    GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT
    GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG
    ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT
    CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA
    CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG
    GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC
    CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG
    GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC
    TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA
    CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA
    ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT
    GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA
    GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC
    ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT
    GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT
    GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT
    TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT
    GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT
    CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC
    TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC
    TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC
    ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC
    AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG
    TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG
    CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC
    CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC
    TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC
    CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC
    AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA
    GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC
    TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA
    GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG
    ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA
    GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC
    CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA
    TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG
    GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC
    ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA
    ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT
    GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC
    AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT
    CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC
    TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC
    ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC
    AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG
    AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT
    CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC
    AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG
    AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT
    GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG
    AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA
    GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG
    TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG
    TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT
    GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC
    TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT
    GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT
    CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC
    ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC
    TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT
    TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA
    GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG
    AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA
    TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG
    GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC
    CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC
    ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG
    GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT
    ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT
    GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG
    ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC
    CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA
    GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG
    GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT
    AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT
    GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT
    CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA
    AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC
    TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG
    GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC
    AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC
    ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC
    TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC
    ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.