MGAM gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015463154.1	XP_015318640.1	maltase-glucoamylase, intestinal isoform X1
XM_015470805.1	XP_015326291.1	maltase-glucoamylase, intestinal isoform X1
XM_015470805.2	XP_015326291.1	maltase-glucoamylase, intestinal isoform X1
XM_024991200.1	XP_024846968.1	maltase-glucoamylase, intestinal isoform X2
XM_015470806.1	XP_015326292.1	maltase-glucoamylase, intestinal isoform X3
XM_015463155.1	XP_015318641.1	maltase-glucoamylase, intestinal isoform X2
XM_005205921.4	XP_005205978.1	maltase-glucoamylase, intestinal isoform X2
XM_024991199.1	XP_024846967.1	maltase-glucoamylase, intestinal isoform X2
XM_005205921.3	XP_005205978.1	maltase-glucoamylase, intestinal isoform X2
XM_015463156.1	XP_015318642.1	maltase-glucoamylase, intestinal isoform X3
XM_024991197.1	XP_024846965.1	maltase-glucoamylase, intestinal isoform X1
XM_024991198.1	XP_024846966.1	maltase-glucoamylase, intestinal isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa89792	XM_015470806.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa89792	XM_015463156.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa234069	XM_024991197.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234069	XM_024991198.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234069	XM_015470805.2 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234070	XM_024991199.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234070	XM_005205921.4 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234070	XM_024991200.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa89791	XM_015463154.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89791	XM_015470805.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa18931	XM_015463155.1 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18931	XM_005205921.3 Latest version!	Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

MGAM ( XM_015470806.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326292.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa89792
Clone ID Related Accession (Same CDS sequence)	XM_015470806.1 , XM_015463156.1
Accession Version	XM_015470806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAG TCGTGGAGAG AAACCGTGCT GCGCAGCTGC CTTATGATGT TCAGCATGCT 
GATATTGATT ACATGGACGC AAGAAAGGAC TTCACTTATG ATCCAGTAGC TTTCAAGGGC 
TTCCCTGAGT TTGTTAAAGA ATTACACAAT AATGGACAGA AACTGGTCAT CATTGTGGAT 
CCTGCCATCT CCAACAACTC TTCCCTAAGT AATCCCTATG GCCCGTATGA CCGGGGTTCA 
GATATGAAGA TATGGGTGAA TACTTCAGAC GGGGTGACTC CACTCATTGG GGAGGTCTGG 
CCTGGAAAAA CTGTGTTTCC TGATTACACC AACCCTAAGT GTACTGCTTG GTGGACAAAT 
GAATTTGAGC TTTTCCACAG TCAAGTAGAG TTTGATGGAA TCTGGATTGA TATGAATGAA 
GTTGCCAACT TTGTGGATGG CTCAGTTTCA GGATGTTCCA CGAGCAATCT AAATTATCCC 
CCATTCACTC CCAAAATCCT GGATGGGTAC CTGTTCAGCA AGAGCATCTG CATGGACGCA 
GTGCAGCACT GGGGGCAGCA TTACGATGTC CACAATCTGT ACGGCTACTC CATGGCCATC 
ACCACTGCAG AAACTGTCAA GACTGTGTTC CCCAACAAGA GAAGCCTCAT TTTAACCCGT 
TCTACGTTTG CGGGTTCTGG CAAGTTTGCA GCCCACTGGC TAGGAGACAA TGCCGCCACC 
TGGAGCGACC TTCGATGGTC CATCCCTGGC ATGCTTGAGT TCAACCTGTT TGGTATCCCC 
ATGGTGGGCG CTGACATATG TGGCTTTATG CTGGATACTT CTGAAGAGCT CTGCAGACGG 
TGGATGCAGC TGGGAGCGTT CTATCCATTT TCCAGGAATC ACAATGGCCA AGGCTACAAG 
GCCCAGGATC CAGCCTCCTT TGGCCCTGAC TCACTGCTGT TGAATTCCTC CAGGCACTAC 
CTGACCATCC GCTATACCCT TCTGCCCTAT CTCTACACAC TCTTCTACCG TGCTCACAGC 
CGCGGGGACA CTGTGGCCAG GCCCCTTCTG CATGAGTTCT ACCAGGACAG CAACACCTGG 
GATGTGCACC AGCAGTTTTT ATGGGGGCCG GGCCTCCTCA TCACTCCAGT CCTGGATGAG 
GGTGCAGAGA AAGTGACCGC ATACATGCCT GACGCTGTCT GGTATGACTA TGAGACTGGG 
GGCCGAGTGA GATGGAGGAA GCAAAAAGTG GAGATGGAAC TTCCTGGAGA CAAAATTGGA 
CTTCACCTGC GAGGAGGCTA CATCTTCCCC ACGCAGCAGC CAGCCACGAC CACGGTGGCC 
AGTCGACGGA ACCCTCTCGG TCTAATCATT GCCCTGGATG AGAACAAAGA AGCAAAAGGA 
GAACTTTTCT GGGATGATGG GGAAACAAAA GATACTGTGG CCAACAACCT TTATCTCTTA 
TGTGAGTTCT CCGTCACCCA AAACCGCCTG GAGGTTAAGA TTCTGCAATC AACCTACACA 
GACCCCAATA ATTTAGCATT TAAAGAAATT AAAATCCTTG GAACCCAGGA GCCTAACAAG 
GTTACAGTGA AACAGAACGG TGTTCCAATT CAAGTTTCTC CTAATGTCAC TTACGATTCC 
AACCTACAGG TGGCCCTTAT CACAGAAATT GATCTTGTTC TGGGAGGAAC ATACACGGTG 
GAGTGGGATG TGAAGATAAG GGATGAAGAA AAAATAGACT GTTATCCTGA TGAGACTGGT 
GTTTCTGCAG AAAACTGCAC TGCCCGCGGT TGTGCCTGGG AGGAGTCCAG TTCTCATGGA 
GTCCCTTTTT GCTATTTCGT CAATGATCTC TACTCTGTCA GTGATGTTCA GTATGACTCA 
CATGGGGCCT CAGCTGTCAT CACCTTAAAG ACTTCTCTGT ATGCCCACTC CTTCCCCTCA 
GTACCAGTGA ACTCCCTGCG CCTGACTGTG ACCTACCATA AGGATAACAT GCTGCAGTTT 
AAGATTTATG ATCCCAGCAA CAACCGGTAT GAAGTTCCAG TCCCTCTCAA CATACCCAGC 
ATTCCATCTG GCACCTCTGA GAGTCAACTC TATGCTGTCC TCATTAAGAA GAATCCATTT 
GGGATTGAAA TTCGCCGCAA GAGTACAGGC ACTGTCATCT GGGACTCTCA GCTCCTCGGC 
TTTACCTTCA ATGACATGTT CATCCGCATC TCCACGCGCC TCCCCTCCAA GTACCTCTAT 
GGCTTTGGGG AAACTGAGCA CACAGCCTTC CGAAGAGACC TGGAATGGAA CACGTGGGGG 
ATGTTCTCCC GAGACCAACC CCCGGGGTAC AAGAAGAACT CCTATGGTGT CCACCCCTAC 
TACATGGCAC TGGAGGAGGA TGGCAGCGCC CACGGAGTGC TCCTGCTGAA CAGCAATGCC 
ATGGATGTGA CATTCCAGCC CTTGCCTGCC TTGACATACC GTACCACAGG AGGAATTCTG 
GACTTTTATG TGGTTTTAGG GCCAACTCCA GAGCTTGTCA CTCAGCAGTA CACTGAGTTG 
ATCGGTCGGC CAGTGATGGT TCCTTACTGG TCCCTGGGGT TTCAGCTGTG TCGCTATGGA 
TACCAGAACG ACTCTGAGAT CGCCAGCTTG TACGATGCTA TGGTGGCTGC ACAGATCCCC 
TATGTATGTC GTCAGCCTGT GGAGTTCTGG CTCTGGGACC TCATGGATGT GCAGTATTCG 
GACATTGACT ACATGGAGCG GCAGCTGGAC TTCACCCTTG ATGCCGAGTT TGAGGGGTTT 
CCAGCTCTGA TTACTCGCAT GAGGGCCGAT GGGATGCGAG TCATTATCAT TCTGGACCCA 
GCCATTTCTG GCAACGAGAC CAAGCCCTAT CTGCCATTCA CACGGGGTGT AGAGGATGAT 
GTCTTCATCA AAGATCCGAG TGATGGGAGC ATTGTCTGGG GAAAGGTCTG GCCTGATTTT 
CCTGATGTGG TAATAAACAG TTCTCTAGAC TGGGACAGTC AAGTGGAGAA ATACCGGGCA 
TTTGTGGCCT TTCCAGACTT TTTCCGTAAT TCAACCACCA CATGGTGGAA GAGGGAGTTG 
AGAGAACTGT ACACCAATCC ACGGGAACCA GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG 
ATTGATATGA ATGAACCAGC GAGCTTCGTG AATGGAGCAG TCCCTCCAGG CTGCAAGGAT 
GCCACTCTGA ACCACCCTCC CTATATGCCA TATCTGGAGT CCAGGGACCG GGGCCTGAGC 
AGCAAGACCC TGTGCATGGA GAGCCAGCAG GTCCTGCCTG ACGGCTCACC GGTGCGCCAC 
TACGACGTGC ACAGCCTCTA CGGGTGGGCC CAGACCAGAC CCACATACGA AGCTGTGCAG 
GAGGTGACGG GACAGCGAGG GATCGTCATC ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC 
TGGGGAGGAC ACTGGCTGGG TGACAACACG GCTGCATGGG ACCAGCTGAA GAAGTCAATC 
ATTGGCATGA TGGAGTTTAG TCTCTTTGGT ATATCCTACA CAGGAGCAGA TATTTGTGGA 
TTCTTTCAAG ATGCTGAATA TGAAATGTGT GCTCGCTGGA TGCAACTAGG GGCCTTCTAC 
CCCTTCGCAA GGAATCACAA TACCATTGGG ACCAAGAGGC AAGACCCCGT GTCCTGGAAT 
TCTACTTTCG TGACAATCTC CAAGAGTGTC CTGGAGACCA GATACACCCT GTTGCCGTAT 
ATCTACACCC TGATGCACAA GGCCAGCACA GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC 
CATGAGTTTG TGTCAGACCG GGTGACCTGG GATGTAGACA GTCAGTTCCT GCTGGGCCCA 
GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT AACGCCAGAG ATGTCACTGC ATATTTCCCT 
AGAGCCCGCT GGTATGATTA CTACACGGGT GTGGACATTC AATCCCGGGG TGAGTGGAAG 
TCCTTGCCGG CACCTCTTGA CCACATTAAT CTTCATGTCC GTGGGGGCTA TGTCCTGCCC 
TGGCAAGAGC CTGCACAGAA CACCCACTTA AGCCGTCAGA AATTCTTAGG TTTCAAGGTT 
GCCTTGGATG ATGAGGGGGC TGCTGAAGGC TGGCTCTTCT GGGATGATGG GCAAAGCATT 
GATACCTATG AAAAAGGACT CTATTACCTG GCGCACTTTT CCGTCAGCCA GAATATGATG 
CACAGTCATA TAATTTTCAA CAACTACATC AGCAATACAA ACCCTTTGAG ACTGGGCTAC 
TTTGAGGTCT GGGGAGTGGG CAGTGACTCC ATTTCCAGTG TCAGCATCTC TGCAAGTGGC 
GTGGTTATAA CACCCACCTT CTCCTACAAC TCTACAACAC AGGTGTTAAA TATTGATGTG 
ACCAGCAGAA ACATCAGCCT ACATAATTTC ACTTCATTGA TGTGGAGAAG CACGCCGAAT 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326292.1
CDS	351..4796
Translation	MKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYVCRQPVEFWLWDLMDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_015470806.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470806.1

ORF Insert Sequence:

ATGAAAGAAG TCGTGGAGAG AAACCGTGCT GCGCAGCTGC CTTATGATGT TCAGCATGCT 
GATATTGATT ACATGGACGC AAGAAAGGAC TTCACTTATG ATCCAGTAGC TTTCAAGGGC 
TTCCCTGAGT TTGTTAAAGA ATTACACAAT AATGGACAGA AACTGGTCAT CATTGTGGAT 
CCTGCCATCT CCAACAACTC TTCCCTAAGT AATCCCTATG GCCCGTATGA CCGGGGTTCA 
GATATGAAGA TATGGGTGAA TACTTCAGAC GGGGTGACTC CACTCATTGG GGAGGTCTGG 
CCTGGAAAAA CTGTGTTTCC TGATTACACC AACCCTAAGT GTACTGCTTG GTGGACAAAT 
GAATTTGAGC TTTTCCACAG TCAAGTAGAG TTTGATGGAA TCTGGATTGA TATGAATGAA 
GTTGCCAACT TTGTGGATGG CTCAGTTTCA GGATGTTCCA CGAGCAATCT AAATTATCCC 
CCATTCACTC CCAAAATCCT GGATGGGTAC CTGTTCAGCA AGAGCATCTG CATGGACGCA 
GTGCAGCACT GGGGGCAGCA TTACGATGTC CACAATCTGT ACGGCTACTC CATGGCCATC 
ACCACTGCAG AAACTGTCAA GACTGTGTTC CCCAACAAGA GAAGCCTCAT TTTAACCCGT 
TCTACGTTTG CGGGTTCTGG CAAGTTTGCA GCCCACTGGC TAGGAGACAA TGCCGCCACC 
TGGAGCGACC TTCGATGGTC CATCCCTGGC ATGCTTGAGT TCAACCTGTT TGGTATCCCC 
ATGGTGGGCG CTGACATATG TGGCTTTATG CTGGATACTT CTGAAGAGCT CTGCAGACGG 
TGGATGCAGC TGGGAGCGTT CTATCCATTT TCCAGGAATC ACAATGGCCA AGGCTACAAG 
GCCCAGGATC CAGCCTCCTT TGGCCCTGAC TCACTGCTGT TGAATTCCTC CAGGCACTAC 
CTGACCATCC GCTATACCCT TCTGCCCTAT CTCTACACAC TCTTCTACCG TGCTCACAGC 
CGCGGGGACA CTGTGGCCAG GCCCCTTCTG CATGAGTTCT ACCAGGACAG CAACACCTGG 
GATGTGCACC AGCAGTTTTT ATGGGGGCCG GGCCTCCTCA TCACTCCAGT CCTGGATGAG 
GGTGCAGAGA AAGTGACCGC ATACATGCCT GACGCTGTCT GGTATGACTA TGAGACTGGG 
GGCCGAGTGA GATGGAGGAA GCAAAAAGTG GAGATGGAAC TTCCTGGAGA CAAAATTGGA 
CTTCACCTGC GAGGAGGCTA CATCTTCCCC ACGCAGCAGC CAGCCACGAC CACGGTGGCC 
AGTCGACGGA ACCCTCTCGG TCTAATCATT GCCCTGGATG AGAACAAAGA AGCAAAAGGA 
GAACTTTTCT GGGATGATGG GGAAACAAAA GATACTGTGG CCAACAACCT TTATCTCTTA 
TGTGAGTTCT CCGTCACCCA AAACCGCCTG GAGGTTAAGA TTCTGCAATC AACCTACACA 
GACCCCAATA ATTTAGCATT TAAAGAAATT AAAATCCTTG GAACCCAGGA GCCTAACAAG 
GTTACAGTGA AACAGAACGG TGTTCCAATT CAAGTTTCTC CTAATGTCAC TTACGATTCC 
AACCTACAGG TGGCCCTTAT CACAGAAATT GATCTTGTTC TGGGAGGAAC ATACACGGTG 
GAGTGGGATG TGAAGATAAG GGATGAAGAA AAAATAGACT GTTATCCTGA TGAGACTGGT 
GTTTCTGCAG AAAACTGCAC TGCCCGCGGT TGTGCCTGGG AGGAGTCCAG TTCTCATGGA 
GTCCCTTTTT GCTATTTCGT CAATGATCTC TACTCTGTCA GTGATGTTCA GTATGACTCA 
CATGGGGCCT CAGCTGTCAT CACCTTAAAG ACTTCTCTGT ATGCCCACTC CTTCCCCTCA 
GTACCAGTGA ACTCCCTGCG CCTGACTGTG ACCTACCATA AGGATAACAT GCTGCAGTTT 
AAGATTTATG ATCCCAGCAA CAACCGGTAT GAAGTTCCAG TCCCTCTCAA CATACCCAGC 
ATTCCATCTG GCACCTCTGA GAGTCAACTC TATGCTGTCC TCATTAAGAA GAATCCATTT 
GGGATTGAAA TTCGCCGCAA GAGTACAGGC ACTGTCATCT GGGACTCTCA GCTCCTCGGC 
TTTACCTTCA ATGACATGTT CATCCGCATC TCCACGCGCC TCCCCTCCAA GTACCTCTAT 
GGCTTTGGGG AAACTGAGCA CACAGCCTTC CGAAGAGACC TGGAATGGAA CACGTGGGGG 
ATGTTCTCCC GAGACCAACC CCCGGGGTAC AAGAAGAACT CCTATGGTGT CCACCCCTAC 
TACATGGCAC TGGAGGAGGA TGGCAGCGCC CACGGAGTGC TCCTGCTGAA CAGCAATGCC 
ATGGATGTGA CATTCCAGCC CTTGCCTGCC TTGACATACC GTACCACAGG AGGAATTCTG 
GACTTTTATG TGGTTTTAGG GCCAACTCCA GAGCTTGTCA CTCAGCAGTA CACTGAGTTG 
ATCGGTCGGC CAGTGATGGT TCCTTACTGG TCCCTGGGGT TTCAGCTGTG TCGCTATGGA 
TACCAGAACG ACTCTGAGAT CGCCAGCTTG TACGATGCTA TGGTGGCTGC ACAGATCCCC 
TATGTATGTC GTCAGCCTGT GGAGTTCTGG CTCTGGGACC TCATGGATGT GCAGTATTCG 
GACATTGACT ACATGGAGCG GCAGCTGGAC TTCACCCTTG ATGCCGAGTT TGAGGGGTTT 
CCAGCTCTGA TTACTCGCAT GAGGGCCGAT GGGATGCGAG TCATTATCAT TCTGGACCCA 
GCCATTTCTG GCAACGAGAC CAAGCCCTAT CTGCCATTCA CACGGGGTGT AGAGGATGAT 
GTCTTCATCA AAGATCCGAG TGATGGGAGC ATTGTCTGGG GAAAGGTCTG GCCTGATTTT 
CCTGATGTGG TAATAAACAG TTCTCTAGAC TGGGACAGTC AAGTGGAGAA ATACCGGGCA 
TTTGTGGCCT TTCCAGACTT TTTCCGTAAT TCAACCACCA CATGGTGGAA GAGGGAGTTG 
AGAGAACTGT ACACCAATCC ACGGGAACCA GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG 
ATTGATATGA ATGAACCAGC GAGCTTCGTG AATGGAGCAG TCCCTCCAGG CTGCAAGGAT 
GCCACTCTGA ACCACCCTCC CTATATGCCA TATCTGGAGT CCAGGGACCG GGGCCTGAGC 
AGCAAGACCC TGTGCATGGA GAGCCAGCAG GTCCTGCCTG ACGGCTCACC GGTGCGCCAC 
TACGACGTGC ACAGCCTCTA CGGGTGGGCC CAGACCAGAC CCACATACGA AGCTGTGCAG 
GAGGTGACGG GACAGCGAGG GATCGTCATC ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC 
TGGGGAGGAC ACTGGCTGGG TGACAACACG GCTGCATGGG ACCAGCTGAA GAAGTCAATC 
ATTGGCATGA TGGAGTTTAG TCTCTTTGGT ATATCCTACA CAGGAGCAGA TATTTGTGGA 
TTCTTTCAAG ATGCTGAATA TGAAATGTGT GCTCGCTGGA TGCAACTAGG GGCCTTCTAC 
CCCTTCGCAA GGAATCACAA TACCATTGGG ACCAAGAGGC AAGACCCCGT GTCCTGGAAT 
TCTACTTTCG TGACAATCTC CAAGAGTGTC CTGGAGACCA GATACACCCT GTTGCCGTAT 
ATCTACACCC TGATGCACAA GGCCAGCACA GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC 
CATGAGTTTG TGTCAGACCG GGTGACCTGG GATGTAGACA GTCAGTTCCT GCTGGGCCCA 
GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT AACGCCAGAG ATGTCACTGC ATATTTCCCT 
AGAGCCCGCT GGTATGATTA CTACACGGGT GTGGACATTC AATCCCGGGG TGAGTGGAAG 
TCCTTGCCGG CACCTCTTGA CCACATTAAT CTTCATGTCC GTGGGGGCTA TGTCCTGCCC 
TGGCAAGAGC CTGCACAGAA CACCCACTTA AGCCGTCAGA AATTCTTAGG TTTCAAGGTT 
GCCTTGGATG ATGAGGGGGC TGCTGAAGGC TGGCTCTTCT GGGATGATGG GCAAAGCATT 
GATACCTATG AAAAAGGACT CTATTACCTG GCGCACTTTT CCGTCAGCCA GAATATGATG 
CACAGTCATA TAATTTTCAA CAACTACATC AGCAATACAA ACCCTTTGAG ACTGGGCTAC 
TTTGAGGTCT GGGGAGTGGG CAGTGACTCC ATTTCCAGTG TCAGCATCTC TGCAAGTGGC 
GTGGTTATAA CACCCACCTT CTCCTACAAC TCTACAACAC AGGTGTTAAA TATTGATGTG 
ACCAGCAGAA ACATCAGCCT ACATAATTTC ACTTCATTGA TGTGGAGAAG CACGCCGAAT 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_015463156.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318642.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa89792
Clone ID Related Accession (Same CDS sequence)	XM_015470806.1 , XM_015463156.1
Accession Version	XM_015463156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAG TCGTGGAGAG AAACCGTGCT GCGCAGCTGC CTTATGATGT TCAGCATGCT 
GATATTGATT ACATGGACGC AAGAAAGGAC TTCACTTATG ATCCAGTAGC TTTCAAGGGC 
TTCCCTGAGT TTGTTAAAGA ATTACACAAT AATGGACAGA AACTGGTCAT CATTGTGGAT 
CCTGCCATCT CCAACAACTC TTCCCTAAGT AATCCCTATG GCCCGTATGA CCGGGGTTCA 
GATATGAAGA TATGGGTGAA TACTTCAGAC GGGGTGACTC CACTCATTGG GGAGGTCTGG 
CCTGGAAAAA CTGTGTTTCC TGATTACACC AACCCTAAGT GTACTGCTTG GTGGACAAAT 
GAATTTGAGC TTTTCCACAG TCAAGTAGAG TTTGATGGAA TCTGGATTGA TATGAATGAA 
GTTGCCAACT TTGTGGATGG CTCAGTTTCA GGATGTTCCA CGAGCAATCT AAATTATCCC 
CCATTCACTC CCAAAATCCT GGATGGGTAC CTGTTCAGCA AGAGCATCTG CATGGACGCA 
GTGCAGCACT GGGGGCAGCA TTACGATGTC CACAATCTGT ACGGCTACTC CATGGCCATC 
ACCACTGCAG AAACTGTCAA GACTGTGTTC CCCAACAAGA GAAGCCTCAT TTTAACCCGT 
TCTACGTTTG CGGGTTCTGG CAAGTTTGCA GCCCACTGGC TAGGAGACAA TGCCGCCACC 
TGGAGCGACC TTCGATGGTC CATCCCTGGC ATGCTTGAGT TCAACCTGTT TGGTATCCCC 
ATGGTGGGCG CTGACATATG TGGCTTTATG CTGGATACTT CTGAAGAGCT CTGCAGACGG 
TGGATGCAGC TGGGAGCGTT CTATCCATTT TCCAGGAATC ACAATGGCCA AGGCTACAAG 
GCCCAGGATC CAGCCTCCTT TGGCCCTGAC TCACTGCTGT TGAATTCCTC CAGGCACTAC 
CTGACCATCC GCTATACCCT TCTGCCCTAT CTCTACACAC TCTTCTACCG TGCTCACAGC 
CGCGGGGACA CTGTGGCCAG GCCCCTTCTG CATGAGTTCT ACCAGGACAG CAACACCTGG 
GATGTGCACC AGCAGTTTTT ATGGGGGCCG GGCCTCCTCA TCACTCCAGT CCTGGATGAG 
GGTGCAGAGA AAGTGACCGC ATACATGCCT GACGCTGTCT GGTATGACTA TGAGACTGGG 
GGCCGAGTGA GATGGAGGAA GCAAAAAGTG GAGATGGAAC TTCCTGGAGA CAAAATTGGA 
CTTCACCTGC GAGGAGGCTA CATCTTCCCC ACGCAGCAGC CAGCCACGAC CACGGTGGCC 
AGTCGACGGA ACCCTCTCGG TCTAATCATT GCCCTGGATG AGAACAAAGA AGCAAAAGGA 
GAACTTTTCT GGGATGATGG GGAAACAAAA GATACTGTGG CCAACAACCT TTATCTCTTA 
TGTGAGTTCT CCGTCACCCA AAACCGCCTG GAGGTTAAGA TTCTGCAATC AACCTACACA 
GACCCCAATA ATTTAGCATT TAAAGAAATT AAAATCCTTG GAACCCAGGA GCCTAACAAG 
GTTACAGTGA AACAGAACGG TGTTCCAATT CAAGTTTCTC CTAATGTCAC TTACGATTCC 
AACCTACAGG TGGCCCTTAT CACAGAAATT GATCTTGTTC TGGGAGGAAC ATACACGGTG 
GAGTGGGATG TGAAGATAAG GGATGAAGAA AAAATAGACT GTTATCCTGA TGAGACTGGT 
GTTTCTGCAG AAAACTGCAC TGCCCGCGGT TGTGCCTGGG AGGAGTCCAG TTCTCATGGA 
GTCCCTTTTT GCTATTTCGT CAATGATCTC TACTCTGTCA GTGATGTTCA GTATGACTCA 
CATGGGGCCT CAGCTGTCAT CACCTTAAAG ACTTCTCTGT ATGCCCACTC CTTCCCCTCA 
GTACCAGTGA ACTCCCTGCG CCTGACTGTG ACCTACCATA AGGATAACAT GCTGCAGTTT 
AAGATTTATG ATCCCAGCAA CAACCGGTAT GAAGTTCCAG TCCCTCTCAA CATACCCAGC 
ATTCCATCTG GCACCTCTGA GAGTCAACTC TATGCTGTCC TCATTAAGAA GAATCCATTT 
GGGATTGAAA TTCGCCGCAA GAGTACAGGC ACTGTCATCT GGGACTCTCA GCTCCTCGGC 
TTTACCTTCA ATGACATGTT CATCCGCATC TCCACGCGCC TCCCCTCCAA GTACCTCTAT 
GGCTTTGGGG AAACTGAGCA CACAGCCTTC CGAAGAGACC TGGAATGGAA CACGTGGGGG 
ATGTTCTCCC GAGACCAACC CCCGGGGTAC AAGAAGAACT CCTATGGTGT CCACCCCTAC 
TACATGGCAC TGGAGGAGGA TGGCAGCGCC CACGGAGTGC TCCTGCTGAA CAGCAATGCC 
ATGGATGTGA CATTCCAGCC CTTGCCTGCC TTGACATACC GTACCACAGG AGGAATTCTG 
GACTTTTATG TGGTTTTAGG GCCAACTCCA GAGCTTGTCA CTCAGCAGTA CACTGAGTTG 
ATCGGTCGGC CAGTGATGGT TCCTTACTGG TCCCTGGGGT TTCAGCTGTG TCGCTATGGA 
TACCAGAACG ACTCTGAGAT CGCCAGCTTG TACGATGCTA TGGTGGCTGC ACAGATCCCC 
TATGTATGTC GTCAGCCTGT GGAGTTCTGG CTCTGGGACC TCATGGATGT GCAGTATTCG 
GACATTGACT ACATGGAGCG GCAGCTGGAC TTCACCCTTG ATGCCGAGTT TGAGGGGTTT 
CCAGCTCTGA TTACTCGCAT GAGGGCCGAT GGGATGCGAG TCATTATCAT TCTGGACCCA 
GCCATTTCTG GCAACGAGAC CAAGCCCTAT CTGCCATTCA CACGGGGTGT AGAGGATGAT 
GTCTTCATCA AAGATCCGAG TGATGGGAGC ATTGTCTGGG GAAAGGTCTG GCCTGATTTT 
CCTGATGTGG TAATAAACAG TTCTCTAGAC TGGGACAGTC AAGTGGAGAA ATACCGGGCA 
TTTGTGGCCT TTCCAGACTT TTTCCGTAAT TCAACCACCA CATGGTGGAA GAGGGAGTTG 
AGAGAACTGT ACACCAATCC ACGGGAACCA GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG 
ATTGATATGA ATGAACCAGC GAGCTTCGTG AATGGAGCAG TCCCTCCAGG CTGCAAGGAT 
GCCACTCTGA ACCACCCTCC CTATATGCCA TATCTGGAGT CCAGGGACCG GGGCCTGAGC 
AGCAAGACCC TGTGCATGGA GAGCCAGCAG GTCCTGCCTG ACGGCTCACC GGTGCGCCAC 
TACGACGTGC ACAGCCTCTA CGGGTGGGCC CAGACCAGAC CCACATACGA AGCTGTGCAG 
GAGGTGACGG GACAGCGAGG GATCGTCATC ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC 
TGGGGAGGAC ACTGGCTGGG TGACAACACG GCTGCATGGG ACCAGCTGAA GAAGTCAATC 
ATTGGCATGA TGGAGTTTAG TCTCTTTGGT ATATCCTACA CAGGAGCAGA TATTTGTGGA 
TTCTTTCAAG ATGCTGAATA TGAAATGTGT GCTCGCTGGA TGCAACTAGG GGCCTTCTAC 
CCCTTCGCAA GGAATCACAA TACCATTGGG ACCAAGAGGC AAGACCCCGT GTCCTGGAAT 
TCTACTTTCG TGACAATCTC CAAGAGTGTC CTGGAGACCA GATACACCCT GTTGCCGTAT 
ATCTACACCC TGATGCACAA GGCCAGCACA GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC 
CATGAGTTTG TGTCAGACCG GGTGACCTGG GATGTAGACA GTCAGTTCCT GCTGGGCCCA 
GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT AACGCCAGAG ATGTCACTGC ATATTTCCCT 
AGAGCCCGCT GGTATGATTA CTACACGGGT GTGGACATTC AATCCCGGGG TGAGTGGAAG 
TCCTTGCCGG CACCTCTTGA CCACATTAAT CTTCATGTCC GTGGGGGCTA TGTCCTGCCC 
TGGCAAGAGC CTGCACAGAA CACCCACTTA AGCCGTCAGA AATTCTTAGG TTTCAAGGTT 
GCCTTGGATG ATGAGGGGGC TGCTGAAGGC TGGCTCTTCT GGGATGATGG GCAAAGCATT 
GATACCTATG AAAAAGGACT CTATTACCTG GCGCACTTTT CCGTCAGCCA GAATATGATG 
CACAGTCATA TAATTTTCAA CAACTACATC AGCAATACAA ACCCTTTGAG ACTGGGCTAC 
TTTGAGGTCT GGGGAGTGGG CAGTGACTCC ATTTCCAGTG TCAGCATCTC TGCAAGTGGC 
GTGGTTATAA CACCCACCTT CTCCTACAAC TCTACAACAC AGGTGTTAAA TATTGATGTG 
ACCAGCAGAA ACATCAGCCT ACATAATTTC ACTTCATTGA TGTGGAGAAG CACGCCGAAT 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318642.1
CDS	351..4796
Translation	MKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYVCRQPVEFWLWDLMDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_015463156.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463156.1

ORF Insert Sequence:

ATGAAAGAAG TCGTGGAGAG AAACCGTGCT GCGCAGCTGC CTTATGATGT TCAGCATGCT 
GATATTGATT ACATGGACGC AAGAAAGGAC TTCACTTATG ATCCAGTAGC TTTCAAGGGC 
TTCCCTGAGT TTGTTAAAGA ATTACACAAT AATGGACAGA AACTGGTCAT CATTGTGGAT 
CCTGCCATCT CCAACAACTC TTCCCTAAGT AATCCCTATG GCCCGTATGA CCGGGGTTCA 
GATATGAAGA TATGGGTGAA TACTTCAGAC GGGGTGACTC CACTCATTGG GGAGGTCTGG 
CCTGGAAAAA CTGTGTTTCC TGATTACACC AACCCTAAGT GTACTGCTTG GTGGACAAAT 
GAATTTGAGC TTTTCCACAG TCAAGTAGAG TTTGATGGAA TCTGGATTGA TATGAATGAA 
GTTGCCAACT TTGTGGATGG CTCAGTTTCA GGATGTTCCA CGAGCAATCT AAATTATCCC 
CCATTCACTC CCAAAATCCT GGATGGGTAC CTGTTCAGCA AGAGCATCTG CATGGACGCA 
GTGCAGCACT GGGGGCAGCA TTACGATGTC CACAATCTGT ACGGCTACTC CATGGCCATC 
ACCACTGCAG AAACTGTCAA GACTGTGTTC CCCAACAAGA GAAGCCTCAT TTTAACCCGT 
TCTACGTTTG CGGGTTCTGG CAAGTTTGCA GCCCACTGGC TAGGAGACAA TGCCGCCACC 
TGGAGCGACC TTCGATGGTC CATCCCTGGC ATGCTTGAGT TCAACCTGTT TGGTATCCCC 
ATGGTGGGCG CTGACATATG TGGCTTTATG CTGGATACTT CTGAAGAGCT CTGCAGACGG 
TGGATGCAGC TGGGAGCGTT CTATCCATTT TCCAGGAATC ACAATGGCCA AGGCTACAAG 
GCCCAGGATC CAGCCTCCTT TGGCCCTGAC TCACTGCTGT TGAATTCCTC CAGGCACTAC 
CTGACCATCC GCTATACCCT TCTGCCCTAT CTCTACACAC TCTTCTACCG TGCTCACAGC 
CGCGGGGACA CTGTGGCCAG GCCCCTTCTG CATGAGTTCT ACCAGGACAG CAACACCTGG 
GATGTGCACC AGCAGTTTTT ATGGGGGCCG GGCCTCCTCA TCACTCCAGT CCTGGATGAG 
GGTGCAGAGA AAGTGACCGC ATACATGCCT GACGCTGTCT GGTATGACTA TGAGACTGGG 
GGCCGAGTGA GATGGAGGAA GCAAAAAGTG GAGATGGAAC TTCCTGGAGA CAAAATTGGA 
CTTCACCTGC GAGGAGGCTA CATCTTCCCC ACGCAGCAGC CAGCCACGAC CACGGTGGCC 
AGTCGACGGA ACCCTCTCGG TCTAATCATT GCCCTGGATG AGAACAAAGA AGCAAAAGGA 
GAACTTTTCT GGGATGATGG GGAAACAAAA GATACTGTGG CCAACAACCT TTATCTCTTA 
TGTGAGTTCT CCGTCACCCA AAACCGCCTG GAGGTTAAGA TTCTGCAATC AACCTACACA 
GACCCCAATA ATTTAGCATT TAAAGAAATT AAAATCCTTG GAACCCAGGA GCCTAACAAG 
GTTACAGTGA AACAGAACGG TGTTCCAATT CAAGTTTCTC CTAATGTCAC TTACGATTCC 
AACCTACAGG TGGCCCTTAT CACAGAAATT GATCTTGTTC TGGGAGGAAC ATACACGGTG 
GAGTGGGATG TGAAGATAAG GGATGAAGAA AAAATAGACT GTTATCCTGA TGAGACTGGT 
GTTTCTGCAG AAAACTGCAC TGCCCGCGGT TGTGCCTGGG AGGAGTCCAG TTCTCATGGA 
GTCCCTTTTT GCTATTTCGT CAATGATCTC TACTCTGTCA GTGATGTTCA GTATGACTCA 
CATGGGGCCT CAGCTGTCAT CACCTTAAAG ACTTCTCTGT ATGCCCACTC CTTCCCCTCA 
GTACCAGTGA ACTCCCTGCG CCTGACTGTG ACCTACCATA AGGATAACAT GCTGCAGTTT 
AAGATTTATG ATCCCAGCAA CAACCGGTAT GAAGTTCCAG TCCCTCTCAA CATACCCAGC 
ATTCCATCTG GCACCTCTGA GAGTCAACTC TATGCTGTCC TCATTAAGAA GAATCCATTT 
GGGATTGAAA TTCGCCGCAA GAGTACAGGC ACTGTCATCT GGGACTCTCA GCTCCTCGGC 
TTTACCTTCA ATGACATGTT CATCCGCATC TCCACGCGCC TCCCCTCCAA GTACCTCTAT 
GGCTTTGGGG AAACTGAGCA CACAGCCTTC CGAAGAGACC TGGAATGGAA CACGTGGGGG 
ATGTTCTCCC GAGACCAACC CCCGGGGTAC AAGAAGAACT CCTATGGTGT CCACCCCTAC 
TACATGGCAC TGGAGGAGGA TGGCAGCGCC CACGGAGTGC TCCTGCTGAA CAGCAATGCC 
ATGGATGTGA CATTCCAGCC CTTGCCTGCC TTGACATACC GTACCACAGG AGGAATTCTG 
GACTTTTATG TGGTTTTAGG GCCAACTCCA GAGCTTGTCA CTCAGCAGTA CACTGAGTTG 
ATCGGTCGGC CAGTGATGGT TCCTTACTGG TCCCTGGGGT TTCAGCTGTG TCGCTATGGA 
TACCAGAACG ACTCTGAGAT CGCCAGCTTG TACGATGCTA TGGTGGCTGC ACAGATCCCC 
TATGTATGTC GTCAGCCTGT GGAGTTCTGG CTCTGGGACC TCATGGATGT GCAGTATTCG 
GACATTGACT ACATGGAGCG GCAGCTGGAC TTCACCCTTG ATGCCGAGTT TGAGGGGTTT 
CCAGCTCTGA TTACTCGCAT GAGGGCCGAT GGGATGCGAG TCATTATCAT TCTGGACCCA 
GCCATTTCTG GCAACGAGAC CAAGCCCTAT CTGCCATTCA CACGGGGTGT AGAGGATGAT 
GTCTTCATCA AAGATCCGAG TGATGGGAGC ATTGTCTGGG GAAAGGTCTG GCCTGATTTT 
CCTGATGTGG TAATAAACAG TTCTCTAGAC TGGGACAGTC AAGTGGAGAA ATACCGGGCA 
TTTGTGGCCT TTCCAGACTT TTTCCGTAAT TCAACCACCA CATGGTGGAA GAGGGAGTTG 
AGAGAACTGT ACACCAATCC ACGGGAACCA GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG 
ATTGATATGA ATGAACCAGC GAGCTTCGTG AATGGAGCAG TCCCTCCAGG CTGCAAGGAT 
GCCACTCTGA ACCACCCTCC CTATATGCCA TATCTGGAGT CCAGGGACCG GGGCCTGAGC 
AGCAAGACCC TGTGCATGGA GAGCCAGCAG GTCCTGCCTG ACGGCTCACC GGTGCGCCAC 
TACGACGTGC ACAGCCTCTA CGGGTGGGCC CAGACCAGAC CCACATACGA AGCTGTGCAG 
GAGGTGACGG GACAGCGAGG GATCGTCATC ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC 
TGGGGAGGAC ACTGGCTGGG TGACAACACG GCTGCATGGG ACCAGCTGAA GAAGTCAATC 
ATTGGCATGA TGGAGTTTAG TCTCTTTGGT ATATCCTACA CAGGAGCAGA TATTTGTGGA 
TTCTTTCAAG ATGCTGAATA TGAAATGTGT GCTCGCTGGA TGCAACTAGG GGCCTTCTAC 
CCCTTCGCAA GGAATCACAA TACCATTGGG ACCAAGAGGC AAGACCCCGT GTCCTGGAAT 
TCTACTTTCG TGACAATCTC CAAGAGTGTC CTGGAGACCA GATACACCCT GTTGCCGTAT 
ATCTACACCC TGATGCACAA GGCCAGCACA GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC 
CATGAGTTTG TGTCAGACCG GGTGACCTGG GATGTAGACA GTCAGTTCCT GCTGGGCCCA 
GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT AACGCCAGAG ATGTCACTGC ATATTTCCCT 
AGAGCCCGCT GGTATGATTA CTACACGGGT GTGGACATTC AATCCCGGGG TGAGTGGAAG 
TCCTTGCCGG CACCTCTTGA CCACATTAAT CTTCATGTCC GTGGGGGCTA TGTCCTGCCC 
TGGCAAGAGC CTGCACAGAA CACCCACTTA AGCCGTCAGA AATTCTTAGG TTTCAAGGTT 
GCCTTGGATG ATGAGGGGGC TGCTGAAGGC TGGCTCTTCT GGGATGATGG GCAAAGCATT 
GATACCTATG AAAAAGGACT CTATTACCTG GCGCACTTTT CCGTCAGCCA GAATATGATG 
CACAGTCATA TAATTTTCAA CAACTACATC AGCAATACAA ACCCTTTGAG ACTGGGCTAC 
TTTGAGGTCT GGGGAGTGGG CAGTGACTCC ATTTCCAGTG TCAGCATCTC TGCAAGTGGC 
GTGGTTATAA CACCCACCTT CTCCTACAAC TCTACAACAC AGGTGTTAAA TATTGATGTG 
ACCAGCAGAA ACATCAGCCT ACATAATTTC ACTTCATTGA TGTGGAGAAG CACGCCGAAT 
TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_015470805.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326291.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

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CloneID	OBa234069
Clone ID Related Accession (Same CDS sequence)	XM_015470805.2 , XM_024991197.1 , XM_024991198.1
Accession Version	XM_015470805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015470805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326291.1
CDS	171..5708
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYVCRQPVEFWLWDLMDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_015470805.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470805.2

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_024991197.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846965.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

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CloneID	OBa234069
Clone ID Related Accession (Same CDS sequence)	XM_015470805.2 , XM_024991197.1 , XM_024991198.1
Accession Version	XM_024991197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846965.1
CDS	255..5792
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYVCRQPVEFWLWDLMDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_024991197.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991197.1

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_024991198.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846966.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

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CloneID	OBa234069
Clone ID Related Accession (Same CDS sequence)	XM_015470805.2 , XM_024991197.1 , XM_024991198.1
Accession Version	XM_024991198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846966.1
CDS	59..5596
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYVCRQPVEFWLWDLMDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_024991198.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991198.1

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_024991200.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846968.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X6, mRNA.

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CloneID	OBa234070
Clone ID Related Accession (Same CDS sequence)	XM_024991200.1 , XM_005205921.4 , XM_024991199.1
Accession Version	XM_024991200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846968.1
CDS	92..5587
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_024991200.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991200.1

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_005205921.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205978.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X4, mRNA.

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CloneID	OBa234070
Clone ID Related Accession (Same CDS sequence)	XM_024991200.1 , XM_005205921.4 , XM_024991199.1
Accession Version	XM_005205921.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205921.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205978.1
CDS	169..5664
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_005205921.4
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205921.4

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_024991199.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846967.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X5, mRNA.

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CloneID	OBa234070
Clone ID Related Accession (Same CDS sequence)	XM_024991200.1 , XM_005205921.4 , XM_024991199.1
Accession Version	XM_024991199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846967.1
CDS	254..5749
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_024991199.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991199.1

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACCTTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_015463154.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318640.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

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CloneID	OBa89791
Clone ID Related Accession (Same CDS sequence)	XM_015463154.1 , XM_015470805.1
Accession Version	XM_015463154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318640.1
CDS	211..5748
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYVCRQPVEFWLWDLMDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_015463154.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463154.1

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_015470805.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326291.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

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CloneID	OBa89791
Clone ID Related Accession (Same CDS sequence)	XM_015463154.1 , XM_015470805.1
Accession Version	XM_015470805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326291.1
CDS	211..5748
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYVCRQPVEFWLWDLMDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_015470805.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470805.1

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGTATG TCGTCAGCCT GTGGAGTTCT GGCTCTGGGA CCTCATGGAT 
GTGCAGTATT CGGACATTGA CTACATGGAG CGGCAGCTGG ACTTCACCCT TGATGCCGAG 
TTTGAGGGGT TTCCAGCTCT GATTACTCGC ATGAGGGCCG ATGGGATGCG AGTCATTATC 
ATTCTGGACC CAGCCATTTC TGGCAACGAG ACCAAGCCCT ATCTGCCATT CACACGGGGT 
GTAGAGGATG ATGTCTTCAT CAAAGATCCG AGTGATGGGA GCATTGTCTG GGGAAAGGTC 
TGGCCTGATT TTCCTGATGT GGTAATAAAC AGTTCTCTAG ACTGGGACAG TCAAGTGGAG 
AAATACCGGG CATTTGTGGC CTTTCCAGAC TTTTTCCGTA ATTCAACCAC CACATGGTGG 
AAGAGGGAGT TGAGAGAACT GTACACCAAT CCACGGGAAC CAGAGAAGAG CTTGAAGTTC 
GATGGCTTGT GGATTGATAT GAATGAACCA GCGAGCTTCG TGAATGGAGC AGTCCCTCCA 
GGCTGCAAGG ATGCCACTCT GAACCACCCT CCCTATATGC CATATCTGGA GTCCAGGGAC 
CGGGGCCTGA GCAGCAAGAC CCTGTGCATG GAGAGCCAGC AGGTCCTGCC TGACGGCTCA 
CCGGTGCGCC ACTACGACGT GCACAGCCTC TACGGGTGGG CCCAGACCAG ACCCACATAC 
GAAGCTGTGC AGGAGGTGAC GGGACAGCGA GGGATCGTCA TCACTCGCTC CACTTTCCCC 
TCTTCTGGCC GCTGGGGAGG ACACTGGCTG GGTGACAACA CGGCTGCATG GGACCAGCTG 
AAGAAGTCAA TCATTGGCAT GATGGAGTTT AGTCTCTTTG GTATATCCTA CACAGGAGCA 
GATATTTGTG GATTCTTTCA AGATGCTGAA TATGAAATGT GTGCTCGCTG GATGCAACTA 
GGGGCCTTCT ACCCCTTCGC AAGGAATCAC AATACCATTG GGACCAAGAG GCAAGACCCC 
GTGTCCTGGA ATTCTACTTT CGTGACAATC TCCAAGAGTG TCCTGGAGAC CAGATACACC 
CTGTTGCCGT ATATCTACAC CCTGATGCAC AAGGCCAGCA CAGAGGGCAG CACCGTCGTG 
CGGCCTCTCC TCCATGAGTT TGTGTCAGAC CGGGTGACCT GGGATGTAGA CAGTCAGTTC 
CTGCTGGGCC CAGCCTTCCT GGTCAGCCCT GTCCTGGAGG CTAACGCCAG AGATGTCACT 
GCATATTTCC CTAGAGCCCG CTGGTATGAT TACTACACGG GTGTGGACAT TCAATCCCGG 
GGTGAGTGGA AGTCCTTGCC GGCACCTCTT GACCACATTA ATCTTCATGT CCGTGGGGGC 
TATGTCCTGC CCTGGCAAGA GCCTGCACAG AACACCCACT TAAGCCGTCA GAAATTCTTA 
GGTTTCAAGG TTGCCTTGGA TGATGAGGGG GCTGCTGAAG GCTGGCTCTT CTGGGATGAT 
GGGCAAAGCA TTGATACCTA TGAAAAAGGA CTCTATTACC TGGCGCACTT TTCCGTCAGC 
CAGAATATGA TGCACAGTCA TATAATTTTC AACAACTACA TCAGCAATAC AAACCCTTTG 
AGACTGGGCT ACTTTGAGGT CTGGGGAGTG GGCAGTGACT CCATTTCCAG TGTCAGCATC 
TCTGCAAGTG GCGTGGTTAT AACACCCACC TTCTCCTACA ACTCTACAAC ACAGGTGTTA 
AATATTGATG TGACCAGCAG AAACATCAGC CTACATAATT TCACTTCATT GATGTGGAGA 
AGCACGCCGA ATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_015463155.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318641.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

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CloneID	OBa18931
Clone ID Related Accession (Same CDS sequence)	XM_015463155.1 , XM_005205921.3
Accession Version	XM_015463155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318641.1
CDS	209..5704
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_015463155.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463155.1

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MGAM ( XM_005205921.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205978.1 Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

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CloneID	OBa18931
Clone ID Related Accession (Same CDS sequence)	XM_015463155.1 , XM_005205921.3
Accession Version	XM_005205921.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maltase-glucoamylase, intestinal isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205978.1
CDS	209..5704
Translation	MARKKLKKFTTLELMLSILLLVVFIITIPIFVLSARDSLESQDPGTTVSPDSGTSTTPGSAECPVVNDSERINCIPDQSPTKATCDQRGCCWSPQGTISMPWCYYSKSHGYQVGGDLVNTNAGFTAQLKRLSSPLFGNDVNNVLLTAEYQTSNRFHFKLTDQNQNRYEVPHEHVQPFTGNAASSLTYKVEVSKQPFGIKVIRTSNNRVLFDSSIGPLLFAHQFLQLSIRLPSANVYGLGEHVHQQYRHDMNWKTWPIFARDTTPNGDGTNLYGAQTFFLCLEDASGLSFGVFLLNSNAMEVFLQPTPAVTYRTIGGILDFYVFLGNTPEQVVQEYLELVGRPALPSYWALGFHLSRYDYGTLDNMKEVVERNRAAQLPYDVQHADIDYMDARKDFTYDPVAFKGFPEFVKELHNNGQKLVIIVDPAISNNSSLSNPYGPYDRGSDMKIWVNTSDGVTPLIGEVWPGKTVFPDYTNPKCTAWWTNEFELFHSQVEFDGIWIDMNEVANFVDGSVSGCSTSNLNYPPFTPKILDGYLFSKSICMDAVQHWGQHYDVHNLYGYSMAITTAETVKTVFPNKRSLILTRSTFAGSGKFAAHWLGDNAATWSDLRWSIPGMLEFNLFGIPMVGADICGFMLDTSEELCRRWMQLGAFYPFSRNHNGQGYKAQDPASFGPDSLLLNSSRHYLTIRYTLLPYLYTLFYRAHSRGDTVARPLLHEFYQDSNTWDVHQQFLWGPGLLITPVLDEGAEKVTAYMPDAVWYDYETGGRVRWRKQKVEMELPGDKIGLHLRGGYIFPTQQPATTTVASRRNPLGLIIALDENKEAKGELFWDDGETKDTVANNLYLLCEFSVTQNRLEVKILQSTYTDPNNLAFKEIKILGTQEPNKVTVKQNGVPIQVSPNVTYDSNLQVALITEIDLVLGGTYTVEWDVKIRDEEKIDCYPDETGVSAENCTARGCAWEESSSHGVPFCYFVNDLYSVSDVQYDSHGASAVITLKTSLYAHSFPSVPVNSLRLTVTYHKDNMLQFKIYDPSNNRYEVPVPLNIPSIPSGTSESQLYAVLIKKNPFGIEIRRKSTGTVIWDSQLLGFTFNDMFIRISTRLPSKYLYGFGETEHTAFRRDLEWNTWGMFSRDQPPGYKKNSYGVHPYYMALEEDGSAHGVLLLNSNAMDVTFQPLPALTYRTTGGILDFYVVLGPTPELVTQQYTELIGRPVMVPYWSLGFQLCRYGYQNDSEIASLYDAMVAAQIPYDVQYSDIDYMERQLDFTLDAEFEGFPALITRMRADGMRVIIILDPAISGNETKPYLPFTRGVEDDVFIKDPSDGSIVWGKVWPDFPDVVINSSLDWDSQVEKYRAFVAFPDFFRNSTTTWWKRELRELYTNPREPEKSLKFDGLWIDMNEPASFVNGAVPPGCKDATLNHPPYMPYLESRDRGLSSKTLCMESQQVLPDGSPVRHYDVHSLYGWAQTRPTYEAVQEVTGQRGIVITRSTFPSSGRWGGHWLGDNTAAWDQLKKSIIGMMEFSLFGISYTGADICGFFQDAEYEMCARWMQLGAFYPFARNHNTIGTKRQDPVSWNSTFVTISKSVLETRYTLLPYIYTLMHKASTEGSTVVRPLLHEFVSDRVTWDVDSQFLLGPAFLVSPVLEANARDVTAYFPRARWYDYYTGVDIQSRGEWKSLPAPLDHINLHVRGGYVLPWQEPAQNTHLSRQKFLGFKVALDDEGAAEGWLFWDDGQSIDTYEKGLYYLAHFSVSQNMMHSHIIFNNYISNTNPLRLGYFEVWGVGSDSISSVSISASGVVITPTFSYNSTTQVLNIDVTSRNISLHNFTSLMWRSTPNF

Target ORF information:

RefSeq Version	XM_005205921.3
Organism	Bos taurus(cattle)
Definition	Bos taurus maltase-glucoamylase (MGAM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205921.3

ORF Insert Sequence:

ATGGCAAGAA AGAAGCTGAA AAAGTTCACT ACTTTGGAGC TCATGCTCAG TATTCTTCTG 
CTTGTGGTGT TTATCATCAC TATTCCCATC TTCGTGCTTT CAGCCAGAGA TTCCCTGGAA 
TCACAAGATC CTGGGACGAC AGTTAGCCCA GATTCTGGGA CAAGTACCAC CCCTGGTTCT 
GCTGAGTGCC CAGTGGTAAA TGACTCTGAA CGGATAAACT GCATCCCTGA CCAATCACCA 
ACAAAGGCTA CCTGTGACCA GCGTGGCTGT TGCTGGAGTC CTCAGGGAAC CATAAGTATG 
CCCTGGTGCT ACTATTCTAA AAGTCATGGC TATCAAGTAG GGGGCGACCT CGTGAACACA 
AATGCAGGAT TCACAGCCCA GCTGAAAAGG TTGTCTTCAC CTCTGTTTGG AAATGATGTC 
AACAATGTCC TTCTCACAGC AGAATACCAG ACATCTAACC GTTTCCATTT TAAGTTAACT 
GACCAAAACC AGAACAGATA CGAAGTGCCC CATGAACATG TGCAACCCTT CACAGGAAAT 
GCTGCCTCTT CCTTGACGTA TAAAGTTGAG GTCTCCAAAC AGCCATTTGG CATCAAAGTG 
ATCAGAACGA GCAACAATCG TGTTCTGTTT GACTCCAGCA TCGGGCCCCT GCTGTTTGCT 
CACCAGTTCC TGCAGCTCTC CATCCGACTG CCTAGTGCCA ACGTGTATGG GCTGGGAGAA 
CACGTGCACC AGCAGTACCG GCATGACATG AACTGGAAGA CCTGGCCCAT ATTTGCCAGG 
GACACAACCC CCAATGGGGA TGGAACTAAC TTGTATGGTG CACAGACGTT CTTCCTATGC 
CTTGAAGATG CTAGTGGATT GTCCTTTGGA GTGTTTCTGC TGAACAGCAA TGCCATGGAG 
GTTTTCCTCC AGCCCACCCC AGCTGTCACT TACCGCACCA TTGGGGGCAT TCTTGACTTC 
TACGTGTTCT TGGGGAATAC TCCAGAGCAA GTTGTTCAAG AATATCTAGA GCTCGTTGGA 
CGGCCAGCCC TACCCTCATA CTGGGCTCTT GGGTTTCATC TCAGTCGTTA TGACTATGGA 
ACCCTAGATA ACATGAAAGA AGTCGTGGAG AGAAACCGTG CTGCGCAGCT GCCTTATGAT 
GTTCAGCATG CTGATATTGA TTACATGGAC GCAAGAAAGG ACTTCACTTA TGATCCAGTA 
GCTTTCAAGG GCTTCCCTGA GTTTGTTAAA GAATTACACA ATAATGGACA GAAACTGGTC 
ATCATTGTGG ATCCTGCCAT CTCCAACAAC TCTTCCCTAA GTAATCCCTA TGGCCCGTAT 
GACCGGGGTT CAGATATGAA GATATGGGTG AATACTTCAG ACGGGGTGAC TCCACTCATT 
GGGGAGGTCT GGCCTGGAAA AACTGTGTTT CCTGATTACA CCAACCCTAA GTGTACTGCT 
TGGTGGACAA ATGAATTTGA GCTTTTCCAC AGTCAAGTAG AGTTTGATGG AATCTGGATT 
GATATGAATG AAGTTGCCAA CTTTGTGGAT GGCTCAGTTT CAGGATGTTC CACGAGCAAT 
CTAAATTATC CCCCATTCAC TCCCAAAATC CTGGATGGGT ACCTGTTCAG CAAGAGCATC 
TGCATGGACG CAGTGCAGCA CTGGGGGCAG CATTACGATG TCCACAATCT GTACGGCTAC 
TCCATGGCCA TCACCACTGC AGAAACTGTC AAGACTGTGT TCCCCAACAA GAGAAGCCTC 
ATTTTAACCC GTTCTACGTT TGCGGGTTCT GGCAAGTTTG CAGCCCACTG GCTAGGAGAC 
AATGCCGCCA CCTGGAGCGA CCTTCGATGG TCCATCCCTG GCATGCTTGA GTTCAACCTG 
TTTGGTATCC CCATGGTGGG CGCTGACATA TGTGGCTTTA TGCTGGATAC TTCTGAAGAG 
CTCTGCAGAC GGTGGATGCA GCTGGGAGCG TTCTATCCAT TTTCCAGGAA TCACAATGGC 
CAAGGCTACA AGGCCCAGGA TCCAGCCTCC TTTGGCCCTG ACTCACTGCT GTTGAATTCC 
TCCAGGCACT ACCTGACCAT CCGCTATACC CTTCTGCCCT ATCTCTACAC ACTCTTCTAC 
CGTGCTCACA GCCGCGGGGA CACTGTGGCC AGGCCCCTTC TGCATGAGTT CTACCAGGAC 
AGCAACACCT GGGATGTGCA CCAGCAGTTT TTATGGGGGC CGGGCCTCCT CATCACTCCA 
GTCCTGGATG AGGGTGCAGA GAAAGTGACC GCATACATGC CTGACGCTGT CTGGTATGAC 
TATGAGACTG GGGGCCGAGT GAGATGGAGG AAGCAAAAAG TGGAGATGGA ACTTCCTGGA 
GACAAAATTG GACTTCACCT GCGAGGAGGC TACATCTTCC CCACGCAGCA GCCAGCCACG 
ACCACGGTGG CCAGTCGACG GAACCCTCTC GGTCTAATCA TTGCCCTGGA TGAGAACAAA 
GAAGCAAAAG GAGAACTTTT CTGGGATGAT GGGGAAACAA AAGATACTGT GGCCAACAAC 
CTTTATCTCT TATGTGAGTT CTCCGTCACC CAAAACCGCC TGGAGGTTAA GATTCTGCAA 
TCAACCTACA CAGACCCCAA TAATTTAGCA TTTAAAGAAA TTAAAATCCT TGGAACCCAG 
GAGCCTAACA AGGTTACAGT GAAACAGAAC GGTGTTCCAA TTCAAGTTTC TCCTAATGTC 
ACTTACGATT CCAACCTACA GGTGGCCCTT ATCACAGAAA TTGATCTTGT TCTGGGAGGA 
ACATACACGG TGGAGTGGGA TGTGAAGATA AGGGATGAAG AAAAAATAGA CTGTTATCCT 
GATGAGACTG GTGTTTCTGC AGAAAACTGC ACTGCCCGCG GTTGTGCCTG GGAGGAGTCC 
AGTTCTCATG GAGTCCCTTT TTGCTATTTC GTCAATGATC TCTACTCTGT CAGTGATGTT 
CAGTATGACT CACATGGGGC CTCAGCTGTC ATCACCTTAA AGACTTCTCT GTATGCCCAC 
TCCTTCCCCT CAGTACCAGT GAACTCCCTG CGCCTGACTG TGACCTACCA TAAGGATAAC 
ATGCTGCAGT TTAAGATTTA TGATCCCAGC AACAACCGGT ATGAAGTTCC AGTCCCTCTC 
AACATACCCA GCATTCCATC TGGCACCTCT GAGAGTCAAC TCTATGCTGT CCTCATTAAG 
AAGAATCCAT TTGGGATTGA AATTCGCCGC AAGAGTACAG GCACTGTCAT CTGGGACTCT 
CAGCTCCTCG GCTTTACCTT CAATGACATG TTCATCCGCA TCTCCACGCG CCTCCCCTCC 
AAGTACCTCT ATGGCTTTGG GGAAACTGAG CACACAGCCT TCCGAAGAGA CCTGGAATGG 
AACACGTGGG GGATGTTCTC CCGAGACCAA CCCCCGGGGT ACAAGAAGAA CTCCTATGGT 
GTCCACCCCT ACTACATGGC ACTGGAGGAG GATGGCAGCG CCCACGGAGT GCTCCTGCTG 
AACAGCAATG CCATGGATGT GACATTCCAG CCCTTGCCTG CCTTGACATA CCGTACCACA 
GGAGGAATTC TGGACTTTTA TGTGGTTTTA GGGCCAACTC CAGAGCTTGT CACTCAGCAG 
TACACTGAGT TGATCGGTCG GCCAGTGATG GTTCCTTACT GGTCCCTGGG GTTTCAGCTG 
TGTCGCTATG GATACCAGAA CGACTCTGAG ATCGCCAGCT TGTACGATGC TATGGTGGCT 
GCACAGATCC CCTATGATGT GCAGTATTCG GACATTGACT ACATGGAGCG GCAGCTGGAC 
TTCACCCTTG ATGCCGAGTT TGAGGGGTTT CCAGCTCTGA TTACTCGCAT GAGGGCCGAT 
GGGATGCGAG TCATTATCAT TCTGGACCCA GCCATTTCTG GCAACGAGAC CAAGCCCTAT 
CTGCCATTCA CACGGGGTGT AGAGGATGAT GTCTTCATCA AAGATCCGAG TGATGGGAGC 
ATTGTCTGGG GAAAGGTCTG GCCTGATTTT CCTGATGTGG TAATAAACAG TTCTCTAGAC 
TGGGACAGTC AAGTGGAGAA ATACCGGGCA TTTGTGGCCT TTCCAGACTT TTTCCGTAAT 
TCAACCACCA CATGGTGGAA GAGGGAGTTG AGAGAACTGT ACACCAATCC ACGGGAACCA 
GAGAAGAGCT TGAAGTTCGA TGGCTTGTGG ATTGATATGA ATGAACCAGC GAGCTTCGTG 
AATGGAGCAG TCCCTCCAGG CTGCAAGGAT GCCACTCTGA ACCACCCTCC CTATATGCCA 
TATCTGGAGT CCAGGGACCG GGGCCTGAGC AGCAAGACCC TGTGCATGGA GAGCCAGCAG 
GTCCTGCCTG ACGGCTCACC GGTGCGCCAC TACGACGTGC ACAGCCTCTA CGGGTGGGCC 
CAGACCAGAC CCACATACGA AGCTGTGCAG GAGGTGACGG GACAGCGAGG GATCGTCATC 
ACTCGCTCCA CTTTCCCCTC TTCTGGCCGC TGGGGAGGAC ACTGGCTGGG TGACAACACG 
GCTGCATGGG ACCAGCTGAA GAAGTCAATC ATTGGCATGA TGGAGTTTAG TCTCTTTGGT 
ATATCCTACA CAGGAGCAGA TATTTGTGGA TTCTTTCAAG ATGCTGAATA TGAAATGTGT 
GCTCGCTGGA TGCAACTAGG GGCCTTCTAC CCCTTCGCAA GGAATCACAA TACCATTGGG 
ACCAAGAGGC AAGACCCCGT GTCCTGGAAT TCTACTTTCG TGACAATCTC CAAGAGTGTC 
CTGGAGACCA GATACACCCT GTTGCCGTAT ATCTACACCC TGATGCACAA GGCCAGCACA 
GAGGGCAGCA CCGTCGTGCG GCCTCTCCTC CATGAGTTTG TGTCAGACCG GGTGACCTGG 
GATGTAGACA GTCAGTTCCT GCTGGGCCCA GCCTTCCTGG TCAGCCCTGT CCTGGAGGCT 
AACGCCAGAG ATGTCACTGC ATATTTCCCT AGAGCCCGCT GGTATGATTA CTACACGGGT 
GTGGACATTC AATCCCGGGG TGAGTGGAAG TCCTTGCCGG CACCTCTTGA CCACATTAAT 
CTTCATGTCC GTGGGGGCTA TGTCCTGCCC TGGCAAGAGC CTGCACAGAA CACCCACTTA 
AGCCGTCAGA AATTCTTAGG TTTCAAGGTT GCCTTGGATG ATGAGGGGGC TGCTGAAGGC 
TGGCTCTTCT GGGATGATGG GCAAAGCATT GATACCTATG AAAAAGGACT CTATTACCTG 
GCGCACTTTT CCGTCAGCCA GAATATGATG CACAGTCATA TAATTTTCAA CAACTACATC 
AGCAATACAA ACCCTTTGAG ACTGGGCTAC TTTGAGGTCT GGGGAGTGGG CAGTGACTCC 
ATTTCCAGTG TCAGCATCTC TGCAAGTGGC GTGGTTATAA CACCCACCTT CTCCTACAAC 
TCTACAACAC AGGTGTTAAA TATTGATGTG ACCAGCAGAA ACATCAGCCT ACATAATTTC 
ACTTCATTGA TGTGGAGAAG CACGCCGAAT TTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MGAM
Entrez Gene ID	100336421
Full Name	maltase-glucoamylase
General protein information	Preferred Names maltase-glucoamylase Names maltase-glucoamylase, intestinal maltase-glucoamylase (alpha-glucosidase)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Mus musculus (house mouse)	Mgam	NP_001164474.1
Xenopus tropicalis (tropical clawed frog)	mgam	XP_002933295.2
Danio rerio (zebrafish)	si	XP_001919135.4
Caenorhabditis elegans (roundworm)	aagr-1	NP_501419.2
Arabidopsis thaliana (thale cress)	AT5G11720	NP_196733.1
Bos taurus (cattle)	MGAM	XP_002687127.1
Rattus norvegicus (Norway rat)	LOC679818	XP_006236432.1
Caenorhabditis elegans (roundworm)	aagr-2	NP_494897.3
Homo sapiens (human)	MGAM	NP_004659.2
Canis lupus familiaris (dog)	MGAM	XP_005629599.1
Gallus gallus (chicken)	SI	XP_422811.3
Neurospora crassa	NCU04674	XP_959217.2
Oryza sativa (rice)	Os06g0676700	NP_001058353.1
Oryza sativa (rice)	Os06g0675700	NP_001058347.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MGAM cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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