IFT140 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010819180.2	XP_010817482.1	intraflagellar transport protein 140 homolog isoform X1
XM_002697913.5	XP_002697959.3	intraflagellar transport protein 140 homolog isoform X1
XM_010825726.2	XP_010824028.1	intraflagellar transport protein 140 homolog isoform X1
XM_015469247.1	XP_015324733.1	intraflagellar transport protein 140 homolog isoform X1
XM_001789788.5	XP_001789840.3	intraflagellar transport protein 140 homolog isoform X1
XM_010819179.3	XP_010817481.1	intraflagellar transport protein 140 homolog isoform X1
XM_015460244.2	XP_015315730.1	intraflagellar transport protein 140 homolog isoform X2
XM_010825723.2	XP_010824025.1	intraflagellar transport protein 140 homolog isoform X1
XM_010819177.2	XP_010817479.1	intraflagellar transport protein 140 homolog isoform X1
XM_015460244.1	XP_015315730.1	intraflagellar transport protein 140 homolog isoform X2
XM_015460243.2	XP_015315729.1	intraflagellar transport protein 140 homolog isoform X1
XM_015460242.1	XP_015315728.1	intraflagellar transport protein 140 homolog isoform X1
XM_010825725.2	XP_010824027.1	intraflagellar transport protein 140 homolog isoform X1
XM_010819179.2	XP_010817481.1	intraflagellar transport protein 140 homolog isoform X1
XM_010819177.3	XP_010817479.1	intraflagellar transport protein 140 homolog isoform X1
XM_015460242.2	XP_015315728.1	intraflagellar transport protein 140 homolog isoform X1
XM_024985375.1	XP_024841143.1	intraflagellar transport protein 140 homolog isoform X1
XM_015460243.1	XP_015315729.1	intraflagellar transport protein 140 homolog isoform X1
XM_015469248.1	XP_015324734.1	intraflagellar transport protein 140 homolog isoform X1
XM_015469249.1	XP_015324735.1	intraflagellar transport protein 140 homolog isoform X2
XM_010819180.3	XP_010817482.1	intraflagellar transport protein 140 homolog isoform X1
XM_002697913.6	XP_002697959.3	intraflagellar transport protein 140 homolog isoform X1
XM_024985376.1	XP_024841144.1	intraflagellar transport protein 140 homolog isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230627	XM_024985376.1 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa11704	XM_010819179.3 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa11704	XM_015460242.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa11704	XM_002697913.6 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa11704	XM_015460243.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa11704	XM_024985375.1 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa11704	XM_010819180.3 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa11704	XM_010819177.3 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa11705	XM_015460244.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_010819179.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_015460243.1 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_002697913.5 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_010825725.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_010825726.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_015469248.1 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_015460242.1 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_015469247.1 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_001789788.5 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_010819180.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_010819177.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32867	XM_010825723.2 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32868	XM_015460244.1 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OBa32868	XM_015469249.1 Latest version!	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

IFT140 ( XM_024985376.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841144.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa230627
Clone ID Related Accession (Same CDS sequence)	XM_024985376.1
Accession Version	XM_024985376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCTACGA GAAGTCGGAC 
ACGCACCGCT TCGAGGTGCC GCGGATGCTG GTGGAGGACC TGCAGGCTCT GGGGCTGTAC 
GTCAACAAGA TGAAGGACAA GACGCTGTGG CGGTGGTGGG CCCAGTACCT GGAGAGCCAG 
GCCAAGATGG ACGCGGCGCT GCACTACTAC GAGCTGGCCC AGGACTACTT CTCCCTGGTC 
CGCATCCACT GCTTCCAGGG CAACATCCAG CGGGCCGCCG AGATAGCCAG CGAGACTGGG 
AACTGGGCGG CCTCCTACCA CCTGGCCCGG CAGTACGAGA GCCAGAACGA GGTGCGGCAG 
GCCGTGCACT TCTACACGCG TGCCCAGGCC TTCAACAACG CCATCCGCCT GTGCAAGGAG 
CACGGCCTGG ACGACCAGCT CATGAACCTG GCCCTGCTGA GCTCCCCCGA GGACATGCTG 
GAGGCCGCGC GCTACTACGA GGAGAGGGGC GAGCAGATGG ACCGGGCCGT CATGCTGTAC 
CACAAGGCCG GCCACTTCTC CAAGGCCCTG GAGCTGGCCT TTGCCACCCA GCAGTTTGCA 
GCCCTGCAGC TGGTGGCAGA GGACCTGGAT GAAAAGTCGG ATCCCGTCCT CCTGGCCCGT 
TGCTCTGACT TCTTCCTGGA GCACGGTCAG TGCGAGAAGG CCGTGGAGCT GCTGCTGGCC 
GCCAAGAAGT ATCAGGAAGC ACTGCAGCTG TGCCTGGAGC AGAACCTGAC CATCACCGAG 
GAGATGGCTG AGAAGATGAC GGTGCCCAAG GACTGCAAGG AGCTGTCCGA GGAGGCCCGG 
CGGGTGCTGC TGGAGCAGAT CGCCAGCTGC TGCATGCGCC AGGGCAGCTA CCACCTGGCC 
ACCAAGAAGT ACACGCAGGC AGGGGACAGG CTGAAGGCCA TGAGGGCGCT GCTCAAGTCT 
GGGGACACGG AGAAGATCGT GTTCTTCGCG GGCGTCTCGC GGCAGAAGGA GATCTATGTC 
ATGGCGGCTA ACTACCTGCA GTCCCTGGAC TGGCGGAAGG ACCCCGAGGT CATGAAGAGC 
ATCATCAGCT TCTACACCAA GGGCCGTGCG CTGGACCTTC TGGCCGGCTT CTACGATGCC 
TGCGCCCAGG TGGAGGTCGA CGAGTACCAG AACTACGACA AGGCACATGG GGCGCTGACC 
GAGGCCTACA AGTGCCTGTC CAAGGCCAAG GCCAGGAGCC CACTGGACCA GGAGGCCAGG 
CTGGCGCAGC TGCAGAGCAG GATGACGCTG GTGAAGCGCT TCATGCAGGC GCGCAGGACC 
TACACGGAGG ACCCCAAGGG GTCGGTCCGG CAGTGCCAGC TGCTCCTGGA AGAGCCGCAC 
CTGGACAGCG GCGTCCGTGT CGGGGACGTC TACGGCTTCC TGGTGGAGCG CCACGTGCAC 
TCGGGAGACC TGCAGACGGC CTACAGGTAC CTGGAGGAGA TGCGGCAGCG GCTGCCCTCC 
GCCAACGTGT CCTACTACGT GAGCCAGCAC ACCGTGGACG CCGTGCACCA GGGCCTGGGG 
CTCCCTCTGA CCTGCGCAGC GCCGAAGCGC GCGCAGCACA GCAGCATGGA GGAGCACAAG 
GAGGCGGACG AGGAGGTGGC CGAGGAGGTG GAAAGTGGGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841144.1
CDS	227..4231
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_024985376.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985376.1

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCTACGA GAAGTCGGAC 
ACGCACCGCT TCGAGGTGCC GCGGATGCTG GTGGAGGACC TGCAGGCTCT GGGGCTGTAC 
GTCAACAAGA TGAAGGACAA GACGCTGTGG CGGTGGTGGG CCCAGTACCT GGAGAGCCAG 
GCCAAGATGG ACGCGGCGCT GCACTACTAC GAGCTGGCCC AGGACTACTT CTCCCTGGTC 
CGCATCCACT GCTTCCAGGG CAACATCCAG CGGGCCGCCG AGATAGCCAG CGAGACTGGG 
AACTGGGCGG CCTCCTACCA CCTGGCCCGG CAGTACGAGA GCCAGAACGA GGTGCGGCAG 
GCCGTGCACT TCTACACGCG TGCCCAGGCC TTCAACAACG CCATCCGCCT GTGCAAGGAG 
CACGGCCTGG ACGACCAGCT CATGAACCTG GCCCTGCTGA GCTCCCCCGA GGACATGCTG 
GAGGCCGCGC GCTACTACGA GGAGAGGGGC GAGCAGATGG ACCGGGCCGT CATGCTGTAC 
CACAAGGCCG GCCACTTCTC CAAGGCCCTG GAGCTGGCCT TTGCCACCCA GCAGTTTGCA 
GCCCTGCAGC TGGTGGCAGA GGACCTGGAT GAAAAGTCGG ATCCCGTCCT CCTGGCCCGT 
TGCTCTGACT TCTTCCTGGA GCACGGTCAG TGCGAGAAGG CCGTGGAGCT GCTGCTGGCC 
GCCAAGAAGT ATCAGGAAGC ACTGCAGCTG TGCCTGGAGC AGAACCTGAC CATCACCGAG 
GAGATGGCTG AGAAGATGAC GGTGCCCAAG GACTGCAAGG AGCTGTCCGA GGAGGCCCGG 
CGGGTGCTGC TGGAGCAGAT CGCCAGCTGC TGCATGCGCC AGGGCAGCTA CCACCTGGCC 
ACCAAGAAGT ACACGCAGGC AGGGGACAGG CTGAAGGCCA TGAGGGCGCT GCTCAAGTCT 
GGGGACACGG AGAAGATCGT GTTCTTCGCG GGCGTCTCGC GGCAGAAGGA GATCTATGTC 
ATGGCGGCTA ACTACCTGCA GTCCCTGGAC TGGCGGAAGG ACCCCGAGGT CATGAAGAGC 
ATCATCAGCT TCTACACCAA GGGCCGTGCG CTGGACCTTC TGGCCGGCTT CTACGATGCC 
TGCGCCCAGG TGGAGGTCGA CGAGTACCAG AACTACGACA AGGCACATGG GGCGCTGACC 
GAGGCCTACA AGTGCCTGTC CAAGGCCAAG GCCAGGAGCC CACTGGACCA GGAGGCCAGG 
CTGGCGCAGC TGCAGAGCAG GATGACGCTG GTGAAGCGCT TCATGCAGGC GCGCAGGACC 
TACACGGAGG ACCCCAAGGG GTCGGTCCGG CAGTGCCAGC TGCTCCTGGA AGAGCCGCAC 
CTGGACAGCG GCGTCCGTGT CGGGGACGTC TACGGCTTCC TGGTGGAGCG CCACGTGCAC 
TCGGGAGACC TGCAGACGGC CTACAGGTAC CTGGAGGAGA TGCGGCAGCG GCTGCCCTCC 
GCCAACGTGT CCTACTACGT GAGCCAGCAC ACCGTGGACG CCGTGCACCA GGGCCTGGGG 
CTCCCTCTGA CCTGCGCAGC GCCGAAGCGC GCGCAGCACA GCAGCATGGA GGAGCACAAG 
GAGGCGGACG AGGAGGTGGC CGAGGAGGTG GAAAGTGGGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010819179.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817481.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa11704
Clone ID Related Accession (Same CDS sequence)	XM_010819179.3 , XM_015460243.2 , XM_010819177.3 , XM_015460242.2 , XM_024985375.1 , XM_010819180.3 , XM_002697913.6
Accession Version	XM_010819179.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817481.1
CDS	228..4601
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010819179.3
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819179.3

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015460243.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315729.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.

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CloneID	OBa11704
Clone ID Related Accession (Same CDS sequence)	XM_010819179.3 , XM_015460243.2 , XM_010819177.3 , XM_015460242.2 , XM_024985375.1 , XM_010819180.3 , XM_002697913.6
Accession Version	XM_015460243.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315729.1
CDS	211..4584
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015460243.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460243.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010819177.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817479.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.

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CloneID	OBa11704
Clone ID Related Accession (Same CDS sequence)	XM_010819179.3 , XM_015460243.2 , XM_010819177.3 , XM_015460242.2 , XM_024985375.1 , XM_010819180.3 , XM_002697913.6
Accession Version	XM_010819177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819177.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817479.1
CDS	171..4544
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010819177.3
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819177.3

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015460242.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315728.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.

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CloneID	OBa11704
Clone ID Related Accession (Same CDS sequence)	XM_010819179.3 , XM_015460243.2 , XM_010819177.3 , XM_015460242.2 , XM_024985375.1 , XM_010819180.3 , XM_002697913.6
Accession Version	XM_015460242.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315728.1
CDS	225..4598
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015460242.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460242.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_024985375.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841143.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa11704
Clone ID Related Accession (Same CDS sequence)	XM_010819179.3 , XM_015460243.2 , XM_010819177.3 , XM_015460242.2 , XM_024985375.1 , XM_010819180.3 , XM_002697913.6
Accession Version	XM_024985375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841143.1
CDS	208..4581
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_024985375.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985375.1

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010819180.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817482.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.

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CloneID	OBa11704
Clone ID Related Accession (Same CDS sequence)	XM_010819179.3 , XM_015460243.2 , XM_010819177.3 , XM_015460242.2 , XM_024985375.1 , XM_010819180.3 , XM_002697913.6
Accession Version	XM_010819180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817482.1
CDS	365..4738
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010819180.3
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819180.3

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_002697913.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697959.3 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.

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CloneID	OBa11704
Clone ID Related Accession (Same CDS sequence)	XM_010819179.3 , XM_015460243.2 , XM_010819177.3 , XM_015460242.2 , XM_024985375.1 , XM_010819180.3 , XM_002697913.6
Accession Version	XM_002697913.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002697913.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697959.3
CDS	186..4559
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_002697913.6
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697913.6

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAA 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015460244.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315730.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X8, mRNA.

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CloneID	OBa11705
Clone ID Related Accession (Same CDS sequence)	XM_015460244.2
Accession Version	XM_015460244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGCAGAC GTGGTGGTCC TGTCCTTCTT CGTCTCAGAA 
GAGCACGGCT TCCTGTTGCA GGACAGCTTC CCCCGTCCCC CTGCCTTCCA GACGCTCCTG 
GGGATCCAGG TGCCCCACTA CTACTTCACG AGAAAGCCAG GAGAAGCTAA TCTGGAGGAC 
CAGCTGGACT CCGGGTCCCA GTGCATCCCC CAGGCAGTGG GCAGGAGGCC CCTGCGGGAC 
TTCGTAGGGC TGGAGGACTG CGACAAGCCC ACGCGGGACG CCATGATCAA CTTCAGCTTT 
TTCCTGACCA TCGGGGACAT GGATGAGGCC TTCAGGTCCA TCAAGCTCAT CAAAAGCCAG 
GCCGTCTGGG AGAACATGGC ACGCATGTGC GTGAAGACTC AGCGGCTGGA CGTGGCCAGG 
GTGTGCCTGG GGCACATGGG CCACGCCCGG GGGGCCCGAG CGCTGCGCGA GGCCCAGCGG 
GAGCCGGAGC CGGAGGCCAG GGTGGCCATG CTGGCCGTGC AGTTGGGCAT GCTGGAGGAC 
GCAGAGCAGC TCTACAGGAG GTGTGGGCGC TACGACCTCC TCAACCGCCT GCTGCAGGCC 
TCAGGCCAGT GGCAGAGGGC CATCGAGGTG GCCGAGCGCC ACGACCGCAT GCACCTGCGC 
ACCACCTACT ACAACTACGC CCGGCACCTG GAGGCCAGCG GGGACTGCAG CCCGGCCCTC 
AGCTACTACG AGAAGTCGGA CACGCACCGC TTCGAGGTGC CGCGGATGCT GGTGGAGGAC 
CTGCAGGCTC TGGGGCTGTA CGTCAACAAG ATGAAGGACA AGACGCTGTG GCGGTGGTGG 
GCCCAGTACC TGGAGAGCCA GGCCAAGATG GACGCGGCGC TGCACTACTA CGAGCTGGCC 
CAGGACTACT TCTCCCTGGT CCGCATCCAC TGCTTCCAGG GCAACATCCA GCGGGCCGCC 
GAGATAGCCA GCGAGACTGG GAACTGGGCG GCCTCCTACC ACCTGGCCCG GCAGTACGAG 
AGCCAGAACG AGGTGCGGCA GGCCGTGCAC TTCTACACGC GTGCCCAGGC CTTCAACAAC 
GCCATCCGCC TGTGCAAGGA GCACGGCCTG GACGACCAGC TCATGAACCT GGCCCTGCTG 
AGCTCCCCCG AGGACATGCT GGAGGCCGCG CGCTACTACG AGGAGAGGGG CGAGCAGATG 
GACCGGGCCG TCATGCTGTA CCACAAGGCC GGCCACTTCT CCAAGGCCCT GGAGCTGGCC 
TTTGCCACCC AGCAGTTTGC AGCCCTGCAG CTGGTGGCAG AGGACCTGGA TGAAAAGTCG 
GATCCCGTCC TCCTGGCCCG TTGCTCTGAC TTCTTCCTGG AGCACGGTCA GTGCGAGAAG 
GCCGTGGAGC TGCTGCTGGC CGCCAAGAAG TATCAGGAAG CACTGCAGCT GTGCCTGGAG 
CAGAACCTGA CCATCACCGA GGAGATGGCT GAGAAGATGA CGGTGCCCAA GGACTGCAAG 
GAGCTGTCCG AGGAGGCCCG GCGGGTGCTG CTGGAGCAGA TCGCCAGCTG CTGCATGCGC 
CAGGGCAGCT ACCACCTGGC CACCAAGAAG TACACGCAGG CAGGGGACAG GCTGAAGGCC 
ATGAGGGCGC TGCTCAAGTC TGGGGACACG GAGAAGATCG TGTTCTTCGC GGGCGTCTCG 
CGGCAGAAGG AGATCTATGT CATGGCGGCT AACTACCTGC AGTCCCTGGA CTGGCGGAAG 
GACCCCGAGG TCATGAAGAG CATCATCAGC TTCTACACCA AGGGCCGTGC GCTGGACCTT 
CTGGCCGGCT TCTACGATGC CTGCGCCCAG GTGGAGGTCG ACGAGTACCA GAACTACGAC 
AAGGCACATG GGGCGCTGAC CGAGGCCTAC AAGTGCCTGT CCAAGGCCAA GGCCAGGAGC 
CCACTGGACC AGGAGGCCAG GCTGGCGCAG CTGCAGAGCA GGATGACGCT GGTGAAGCGC 
TTCATGCAGG CGCGCAGGAC CTACACGGAG GACCCCAAGG GGTCGGTCCG GCAGTGCCAG 
CTGCTCCTGG AAGAGCCGCA CCTGGACAGC GGCGTCCGTG TCGGGGACGT CTACGGCTTC 
CTGGTGGAGC GCCACGTGCA CTCGGGAGAC CTGCAGACGG CCTACAGGTA CCTGGAGGAG 
ATGCGGCAGC GGCTGCCCTC CGCCAACGTG TCCTACTACG TGAGCCAGCA CACCGTGGAC 
GCCGTGCACC AGGGCCTGGG GCTCCCTCTG ACCTGCGCAG CGCCGAAGCG CGCGCAGCAC 
AGCAGCATGG AGGAGCACAA GGAGGCGGAC GAGGAGGTGG CCGAGGAGGT GGAAAGTGGG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315730.1
CDS	227..4552
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015460244.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460244.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCACGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGCAGAC GTGGTGGTCC TGTCCTTCTT CGTCTCAGAA 
GAGCACGGCT TCCTGTTGCA GGACAGCTTC CCCCGTCCCC CTGCCTTCCA GACGCTCCTG 
GGGATCCAGG TGCCCCACTA CTACTTCACG AGAAAGCCAG GAGAAGCTAA TCTGGAGGAC 
CAGCTGGACT CCGGGTCCCA GTGCATCCCC CAGGCAGTGG GCAGGAGGCC CCTGCGGGAC 
TTCGTAGGGC TGGAGGACTG CGACAAGCCC ACGCGGGACG CCATGATCAA CTTCAGCTTT 
TTCCTGACCA TCGGGGACAT GGATGAGGCC TTCAGGTCCA TCAAGCTCAT CAAAAGCCAG 
GCCGTCTGGG AGAACATGGC ACGCATGTGC GTGAAGACTC AGCGGCTGGA CGTGGCCAGG 
GTGTGCCTGG GGCACATGGG CCACGCCCGG GGGGCCCGAG CGCTGCGCGA GGCCCAGCGG 
GAGCCGGAGC CGGAGGCCAG GGTGGCCATG CTGGCCGTGC AGTTGGGCAT GCTGGAGGAC 
GCAGAGCAGC TCTACAGGAG GTGTGGGCGC TACGACCTCC TCAACCGCCT GCTGCAGGCC 
TCAGGCCAGT GGCAGAGGGC CATCGAGGTG GCCGAGCGCC ACGACCGCAT GCACCTGCGC 
ACCACCTACT ACAACTACGC CCGGCACCTG GAGGCCAGCG GGGACTGCAG CCCGGCCCTC 
AGCTACTACG AGAAGTCGGA CACGCACCGC TTCGAGGTGC CGCGGATGCT GGTGGAGGAC 
CTGCAGGCTC TGGGGCTGTA CGTCAACAAG ATGAAGGACA AGACGCTGTG GCGGTGGTGG 
GCCCAGTACC TGGAGAGCCA GGCCAAGATG GACGCGGCGC TGCACTACTA CGAGCTGGCC 
CAGGACTACT TCTCCCTGGT CCGCATCCAC TGCTTCCAGG GCAACATCCA GCGGGCCGCC 
GAGATAGCCA GCGAGACTGG GAACTGGGCG GCCTCCTACC ACCTGGCCCG GCAGTACGAG 
AGCCAGAACG AGGTGCGGCA GGCCGTGCAC TTCTACACGC GTGCCCAGGC CTTCAACAAC 
GCCATCCGCC TGTGCAAGGA GCACGGCCTG GACGACCAGC TCATGAACCT GGCCCTGCTG 
AGCTCCCCCG AGGACATGCT GGAGGCCGCG CGCTACTACG AGGAGAGGGG CGAGCAGATG 
GACCGGGCCG TCATGCTGTA CCACAAGGCC GGCCACTTCT CCAAGGCCCT GGAGCTGGCC 
TTTGCCACCC AGCAGTTTGC AGCCCTGCAG CTGGTGGCAG AGGACCTGGA TGAAAAGTCG 
GATCCCGTCC TCCTGGCCCG TTGCTCTGAC TTCTTCCTGG AGCACGGTCA GTGCGAGAAG 
GCCGTGGAGC TGCTGCTGGC CGCCAAGAAG TATCAGGAAG CACTGCAGCT GTGCCTGGAG 
CAGAACCTGA CCATCACCGA GGAGATGGCT GAGAAGATGA CGGTGCCCAA GGACTGCAAG 
GAGCTGTCCG AGGAGGCCCG GCGGGTGCTG CTGGAGCAGA TCGCCAGCTG CTGCATGCGC 
CAGGGCAGCT ACCACCTGGC CACCAAGAAG TACACGCAGG CAGGGGACAG GCTGAAGGCC 
ATGAGGGCGC TGCTCAAGTC TGGGGACACG GAGAAGATCG TGTTCTTCGC GGGCGTCTCG 
CGGCAGAAGG AGATCTATGT CATGGCGGCT AACTACCTGC AGTCCCTGGA CTGGCGGAAG 
GACCCCGAGG TCATGAAGAG CATCATCAGC TTCTACACCA AGGGCCGTGC GCTGGACCTT 
CTGGCCGGCT TCTACGATGC CTGCGCCCAG GTGGAGGTCG ACGAGTACCA GAACTACGAC 
AAGGCACATG GGGCGCTGAC CGAGGCCTAC AAGTGCCTGT CCAAGGCCAA GGCCAGGAGC 
CCACTGGACC AGGAGGCCAG GCTGGCGCAG CTGCAGAGCA GGATGACGCT GGTGAAGCGC 
TTCATGCAGG CGCGCAGGAC CTACACGGAG GACCCCAAGG GGTCGGTCCG GCAGTGCCAG 
CTGCTCCTGG AAGAGCCGCA CCTGGACAGC GGCGTCCGTG TCGGGGACGT CTACGGCTTC 
CTGGTGGAGC GCCACGTGCA CTCGGGAGAC CTGCAGACGG CCTACAGGTA CCTGGAGGAG 
ATGCGGCAGC GGCTGCCCTC CGCCAACGTG TCCTACTACG TGAGCCAGCA CACCGTGGAC 
GCCGTGCACC AGGGCCTGGG GCTCCCTCTG ACCTGCGCAG CGCCGAAGCG CGCGCAGCAC 
AGCAGCATGG AGGAGCACAA GGAGGCGGAC GAGGAGGTGG CCGAGGAGGT GGAAAGTGGG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010819180.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817482.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_010819180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817482.1
CDS	341..4714
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010819180.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819180.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_002697913.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697959.3 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.

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CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_002697913.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697913.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697959.3
CDS	186..4559
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_002697913.5
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697913.5

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010825726.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824028.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.

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CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_010825726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824028.1
CDS	341..4714
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010825726.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825726.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015469247.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324733.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.

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CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_015469247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324733.1
CDS	389..4762
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015469247.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469247.1

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_001789788.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_001789840.3 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_001789788.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001789788.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001789840.3
CDS	186..4559
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_001789788.5
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001789788.5

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010825723.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824025.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_010825723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824025.1
CDS	171..4544
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010825723.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825723.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010819177.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817479.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.

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CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_010819177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817479.1
CDS	171..4544
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010819177.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819177.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015460242.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315728.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.

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CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_015460242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315728.1
CDS	389..4762
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015460242.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460242.1

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010825725.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824027.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_010825725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824027.1
CDS	238..4611
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010825725.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825725.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_010819179.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817481.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_010819179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817481.1
CDS	238..4611
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_010819179.2
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819179.2

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015460243.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315729.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.

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CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_015460243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315729.1
CDS	375..4748
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015460243.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460243.1

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015469248.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324734.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.

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CloneID	OBa32867
Clone ID Related Accession (Same CDS sequence)	XM_010819180.2 , XM_002697913.5 , XM_010825726.2 , XM_015469247.1 , XM_001789788.5 , XM_010825723.2 , XM_010819177.2 , XM_015460242.1 , XM_010825725.2 , XM_010819179.2 , XM_015460243.1 , XM_015469248.1
Accession Version	XM_015469248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324734.1
CDS	375..4748
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREVPGAPPQPADRNALAEADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015469248.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469248.1

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGTGCCC GGCGCCCCAC CACAGCCCGC GGACAGGAAC 
GCGCTGGCCG AGGCAGACGT GGTGGTCCTG TCCTTCTTCG TCTCAGAAGA GCACGGCTTC 
CTGTTGCAGG ACAGCTTCCC CCGTCCCCCT GCCTTCCAGA CGCTCCTGGG GATCCAGGTG 
CCCCACTACT ACTTCACGAG AAAGCCAGGA GAAGCTAATC TGGAGGACCA GCTGGACTCC 
GGGTCCCAGT GCATCCCCCA GGCAGTGGGC AGGAGGCCCC TGCGGGACTT CGTAGGGCTG 
GAGGACTGCG ACAAGCCCAC GCGGGACGCC ATGATCAACT TCAGCTTTTT CCTGACCATC 
GGGGACATGG ATGAGGCCTT CAGGTCCATC AAGCTCATCA AAAGCCAGGC CGTCTGGGAG 
AACATGGCAC GCATGTGCGT GAAGACTCAG CGGCTGGACG TGGCCAGGGT GTGCCTGGGG 
CACATGGGCC ACGCCCGGGG GGCCCGAGCG CTGCGCGAGG CCCAGCGGGA GCCGGAGCCG 
GAGGCCAGGG TGGCCATGCT GGCCGTGCAG TTGGGCATGC TGGAGGACGC AGAGCAGCTC 
TACAGGAGGT GTGGGCGCTA CGACCTCCTC AACCGCCTGC TGCAGGCCTC AGGCCAGTGG 
CAGAGGGCCA TCGAGGTGGC CGAGCGCCAC GACCGCATGC ACCTGCGCAC CACCTACTAC 
AACTACGCCC GGCACCTGGA GGCCAGCGGG GACTGCAGCC CGGCCCTCAG CTACTACGAG 
AAGTCGGACA CGCACCGCTT CGAGGTGCCG CGGATGCTGG TGGAGGACCT GCAGGCTCTG 
GGGCTGTACG TCAACAAGAT GAAGGACAAG ACGCTGTGGC GGTGGTGGGC CCAGTACCTG 
GAGAGCCAGG CCAAGATGGA CGCGGCGCTG CACTACTACG AGCTGGCCCA GGACTACTTC 
TCCCTGGTCC GCATCCACTG CTTCCAGGGC AACATCCAGC GGGCCGCCGA GATAGCCAGC 
GAGACTGGGA ACTGGGCGGC CTCCTACCAC CTGGCCCGGC AGTACGAGAG CCAGAACGAG 
GTGCGGCAGG CCGTGCACTT CTACACGCGT GCCCAGGCCT TCAACAACGC CATCCGCCTG 
TGCAAGGAGC ACGGCCTGGA CGACCAGCTC ATGAACCTGG CCCTGCTGAG CTCCCCCGAG 
GACATGCTGG AGGCCGCGCG CTACTACGAG GAGAGGGGCG AGCAGATGGA CCGGGCCGTC 
ATGCTGTACC ACAAGGCCGG CCACTTCTCC AAGGCCCTGG AGCTGGCCTT TGCCACCCAG 
CAGTTTGCAG CCCTGCAGCT GGTGGCAGAG GACCTGGATG AAAAGTCGGA TCCCGTCCTC 
CTGGCCCGTT GCTCTGACTT CTTCCTGGAG CACGGTCAGT GCGAGAAGGC CGTGGAGCTG 
CTGCTGGCCG CCAAGAAGTA TCAGGAAGCA CTGCAGCTGT GCCTGGAGCA GAACCTGACC 
ATCACCGAGG AGATGGCTGA GAAGATGACG GTGCCCAAGG ACTGCAAGGA GCTGTCCGAG 
GAGGCCCGGC GGGTGCTGCT GGAGCAGATC GCCAGCTGCT GCATGCGCCA GGGCAGCTAC 
CACCTGGCCA CCAAGAAGTA CACGCAGGCA GGGGACAGGC TGAAGGCCAT GAGGGCGCTG 
CTCAAGTCTG GGGACACGGA GAAGATCGTG TTCTTCGCGG GCGTCTCGCG GCAGAAGGAG 
ATCTATGTCA TGGCGGCTAA CTACCTGCAG TCCCTGGACT GGCGGAAGGA CCCCGAGGTC 
ATGAAGAGCA TCATCAGCTT CTACACCAAG GGCCGTGCGC TGGACCTTCT GGCCGGCTTC 
TACGATGCCT GCGCCCAGGT GGAGGTCGAC GAGTACCAGA ACTACGACAA GGCACATGGG 
GCGCTGACCG AGGCCTACAA GTGCCTGTCC AAGGCCAAGG CCAGGAGCCC ACTGGACCAG 
GAGGCCAGGC TGGCGCAGCT GCAGAGCAGG ATGACGCTGG TGAAGCGCTT CATGCAGGCG 
CGCAGGACCT ACACGGAGGA CCCCAAGGGG TCGGTCCGGC AGTGCCAGCT GCTCCTGGAG 
GAGCCGCACC TGGACAGCGG CGTCCGTGTC GGGGACGTCT ACGGCTTCCT GGTGGAGCGC 
CACGTGCACT CGGGAGACCT GCAGACGGCC TACAGGTACC TGGAGGAGAT GCGGCAGCGG 
CTGCCCTCCG CCAACGTGTC CTACTACGTG AGCCAGCACA CCGTGGACGC CGTGCACCAG 
GGCCTGGGGC TCCCTCTGAC CTGCGCAGCG CCGAAGCGCG CGCAGCACAG CAGCATGGAG 
GAGCACAAGG AGGCGGACGA GGAGGTGGCC GAGGAGGTGG AAAGTGGGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015460244.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015315730.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa32868
Clone ID Related Accession (Same CDS sequence)	XM_015460244.1 , XM_015469249.1
Accession Version	XM_015460244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGCAGAC GTGGTGGTCC TGTCCTTCTT CGTCTCAGAA 
GAGCACGGCT TCCTGTTGCA GGACAGCTTC CCCCGTCCCC CTGCCTTCCA GACGCTCCTG 
GGGATCCAGG TGCCCCACTA CTACTTCACG AGAAAGCCAG GAGAAGCTAA TCTGGAGGAC 
CAGCTGGACT CCGGGTCCCA GTGCATCCCC CAGGCAGTGG GCAGGAGGCC CCTGCGGGAC 
TTCGTAGGGC TGGAGGACTG CGACAAGCCC ACGCGGGACG CCATGATCAA CTTCAGCTTT 
TTCCTGACCA TCGGGGACAT GGATGAGGCC TTCAGGTCCA TCAAGCTCAT CAAAAGCCAG 
GCCGTCTGGG AGAACATGGC ACGCATGTGC GTGAAGACTC AGCGGCTGGA CGTGGCCAGG 
GTGTGCCTGG GGCACATGGG CCACGCCCGG GGGGCCCGAG CGCTGCGCGA GGCCCAGCGG 
GAGCCGGAGC CGGAGGCCAG GGTGGCCATG CTGGCCGTGC AGTTGGGCAT GCTGGAGGAC 
GCAGAGCAGC TCTACAGGAG GTGTGGGCGC TACGACCTCC TCAACCGCCT GCTGCAGGCC 
TCAGGCCAGT GGCAGAGGGC CATCGAGGTG GCCGAGCGCC ACGACCGCAT GCACCTGCGC 
ACCACCTACT ACAACTACGC CCGGCACCTG GAGGCCAGCG GGGACTGCAG CCCGGCCCTC 
AGCTACTACG AGAAGTCGGA CACGCACCGC TTCGAGGTGC CGCGGATGCT GGTGGAGGAC 
CTGCAGGCTC TGGGGCTGTA CGTCAACAAG ATGAAGGACA AGACGCTGTG GCGGTGGTGG 
GCCCAGTACC TGGAGAGCCA GGCCAAGATG GACGCGGCGC TGCACTACTA CGAGCTGGCC 
CAGGACTACT TCTCCCTGGT CCGCATCCAC TGCTTCCAGG GCAACATCCA GCGGGCCGCC 
GAGATAGCCA GCGAGACTGG GAACTGGGCG GCCTCCTACC ACCTGGCCCG GCAGTACGAG 
AGCCAGAACG AGGTGCGGCA GGCCGTGCAC TTCTACACGC GTGCCCAGGC CTTCAACAAC 
GCCATCCGCC TGTGCAAGGA GCACGGCCTG GACGACCAGC TCATGAACCT GGCCCTGCTG 
AGCTCCCCCG AGGACATGCT GGAGGCCGCG CGCTACTACG AGGAGAGGGG CGAGCAGATG 
GACCGGGCCG TCATGCTGTA CCACAAGGCC GGCCACTTCT CCAAGGCCCT GGAGCTGGCC 
TTTGCCACCC AGCAGTTTGC AGCCCTGCAG CTGGTGGCAG AGGACCTGGA TGAAAAGTCG 
GATCCCGTCC TCCTGGCCCG TTGCTCTGAC TTCTTCCTGG AGCACGGTCA GTGCGAGAAG 
GCCGTGGAGC TGCTGCTGGC CGCCAAGAAG TATCAGGAAG CACTGCAGCT GTGCCTGGAG 
CAGAACCTGA CCATCACCGA GGAGATGGCT GAGAAGATGA CGGTGCCCAA GGACTGCAAG 
GAGCTGTCCG AGGAGGCCCG GCGGGTGCTG CTGGAGCAGA TCGCCAGCTG CTGCATGCGC 
CAGGGCAGCT ACCACCTGGC CACCAAGAAG TACACGCAGG CAGGGGACAG GCTGAAGGCC 
ATGAGGGCGC TGCTCAAGTC TGGGGACACG GAGAAGATCG TGTTCTTCGC GGGCGTCTCG 
CGGCAGAAGG AGATCTATGT CATGGCGGCT AACTACCTGC AGTCCCTGGA CTGGCGGAAG 
GACCCCGAGG TCATGAAGAG CATCATCAGC TTCTACACCA AGGGCCGTGC GCTGGACCTT 
CTGGCCGGCT TCTACGATGC CTGCGCCCAG GTGGAGGTCG ACGAGTACCA GAACTACGAC 
AAGGCACATG GGGCGCTGAC CGAGGCCTAC AAGTGCCTGT CCAAGGCCAA GGCCAGGAGC 
CCACTGGACC AGGAGGCCAG GCTGGCGCAG CTGCAGAGCA GGATGACGCT GGTGAAGCGC 
TTCATGCAGG CGCGCAGGAC CTACACGGAG GACCCCAAGG GGTCGGTCCG GCAGTGCCAG 
CTGCTCCTGG AGGAGCCGCA CCTGGACAGC GGCGTCCGTG TCGGGGACGT CTACGGCTTC 
CTGGTGGAGC GCCACGTGCA CTCGGGAGAC CTGCAGACGG CCTACAGGTA CCTGGAGGAG 
ATGCGGCAGC GGCTGCCCTC CGCCAACGTG TCCTACTACG TGAGCCAGCA CACCGTGGAC 
GCCGTGCACC AGGGCCTGGG GCTCCCTCTG ACCTGCGCAG CGCCGAAGCG CGCGCAGCAC 
AGCAGCATGG AGGAGCACAA GGAGGCGGAC GAGGAGGTGG CCGAGGAGGT GGAAAGTGGG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015315730.1
CDS	392..4717
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015460244.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015460244.1

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGCAGAC GTGGTGGTCC TGTCCTTCTT CGTCTCAGAA 
GAGCACGGCT TCCTGTTGCA GGACAGCTTC CCCCGTCCCC CTGCCTTCCA GACGCTCCTG 
GGGATCCAGG TGCCCCACTA CTACTTCACG AGAAAGCCAG GAGAAGCTAA TCTGGAGGAC 
CAGCTGGACT CCGGGTCCCA GTGCATCCCC CAGGCAGTGG GCAGGAGGCC CCTGCGGGAC 
TTCGTAGGGC TGGAGGACTG CGACAAGCCC ACGCGGGACG CCATGATCAA CTTCAGCTTT 
TTCCTGACCA TCGGGGACAT GGATGAGGCC TTCAGGTCCA TCAAGCTCAT CAAAAGCCAG 
GCCGTCTGGG AGAACATGGC ACGCATGTGC GTGAAGACTC AGCGGCTGGA CGTGGCCAGG 
GTGTGCCTGG GGCACATGGG CCACGCCCGG GGGGCCCGAG CGCTGCGCGA GGCCCAGCGG 
GAGCCGGAGC CGGAGGCCAG GGTGGCCATG CTGGCCGTGC AGTTGGGCAT GCTGGAGGAC 
GCAGAGCAGC TCTACAGGAG GTGTGGGCGC TACGACCTCC TCAACCGCCT GCTGCAGGCC 
TCAGGCCAGT GGCAGAGGGC CATCGAGGTG GCCGAGCGCC ACGACCGCAT GCACCTGCGC 
ACCACCTACT ACAACTACGC CCGGCACCTG GAGGCCAGCG GGGACTGCAG CCCGGCCCTC 
AGCTACTACG AGAAGTCGGA CACGCACCGC TTCGAGGTGC CGCGGATGCT GGTGGAGGAC 
CTGCAGGCTC TGGGGCTGTA CGTCAACAAG ATGAAGGACA AGACGCTGTG GCGGTGGTGG 
GCCCAGTACC TGGAGAGCCA GGCCAAGATG GACGCGGCGC TGCACTACTA CGAGCTGGCC 
CAGGACTACT TCTCCCTGGT CCGCATCCAC TGCTTCCAGG GCAACATCCA GCGGGCCGCC 
GAGATAGCCA GCGAGACTGG GAACTGGGCG GCCTCCTACC ACCTGGCCCG GCAGTACGAG 
AGCCAGAACG AGGTGCGGCA GGCCGTGCAC TTCTACACGC GTGCCCAGGC CTTCAACAAC 
GCCATCCGCC TGTGCAAGGA GCACGGCCTG GACGACCAGC TCATGAACCT GGCCCTGCTG 
AGCTCCCCCG AGGACATGCT GGAGGCCGCG CGCTACTACG AGGAGAGGGG CGAGCAGATG 
GACCGGGCCG TCATGCTGTA CCACAAGGCC GGCCACTTCT CCAAGGCCCT GGAGCTGGCC 
TTTGCCACCC AGCAGTTTGC AGCCCTGCAG CTGGTGGCAG AGGACCTGGA TGAAAAGTCG 
GATCCCGTCC TCCTGGCCCG TTGCTCTGAC TTCTTCCTGG AGCACGGTCA GTGCGAGAAG 
GCCGTGGAGC TGCTGCTGGC CGCCAAGAAG TATCAGGAAG CACTGCAGCT GTGCCTGGAG 
CAGAACCTGA CCATCACCGA GGAGATGGCT GAGAAGATGA CGGTGCCCAA GGACTGCAAG 
GAGCTGTCCG AGGAGGCCCG GCGGGTGCTG CTGGAGCAGA TCGCCAGCTG CTGCATGCGC 
CAGGGCAGCT ACCACCTGGC CACCAAGAAG TACACGCAGG CAGGGGACAG GCTGAAGGCC 
ATGAGGGCGC TGCTCAAGTC TGGGGACACG GAGAAGATCG TGTTCTTCGC GGGCGTCTCG 
CGGCAGAAGG AGATCTATGT CATGGCGGCT AACTACCTGC AGTCCCTGGA CTGGCGGAAG 
GACCCCGAGG TCATGAAGAG CATCATCAGC TTCTACACCA AGGGCCGTGC GCTGGACCTT 
CTGGCCGGCT TCTACGATGC CTGCGCCCAG GTGGAGGTCG ACGAGTACCA GAACTACGAC 
AAGGCACATG GGGCGCTGAC CGAGGCCTAC AAGTGCCTGT CCAAGGCCAA GGCCAGGAGC 
CCACTGGACC AGGAGGCCAG GCTGGCGCAG CTGCAGAGCA GGATGACGCT GGTGAAGCGC 
TTCATGCAGG CGCGCAGGAC CTACACGGAG GACCCCAAGG GGTCGGTCCG GCAGTGCCAG 
CTGCTCCTGG AGGAGCCGCA CCTGGACAGC GGCGTCCGTG TCGGGGACGT CTACGGCTTC 
CTGGTGGAGC GCCACGTGCA CTCGGGAGAC CTGCAGACGG CCTACAGGTA CCTGGAGGAG 
ATGCGGCAGC GGCTGCCCTC CGCCAACGTG TCCTACTACG TGAGCCAGCA CACCGTGGAC 
GCCGTGCACC AGGGCCTGGG GCTCCCTCTG ACCTGCGCAG CGCCGAAGCG CGCGCAGCAC 
AGCAGCATGG AGGAGCACAA GGAGGCGGAC GAGGAGGTGG CCGAGGAGGT GGAAAGTGGG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IFT140 ( XM_015469249.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324735.1 Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa32868
Clone ID Related Accession (Same CDS sequence)	XM_015460244.1 , XM_015469249.1
Accession Version	XM_015469249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	intraflagellar transport protein 140 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGCAGAC GTGGTGGTCC TGTCCTTCTT CGTCTCAGAA 
GAGCACGGCT TCCTGTTGCA GGACAGCTTC CCCCGTCCCC CTGCCTTCCA GACGCTCCTG 
GGGATCCAGG TGCCCCACTA CTACTTCACG AGAAAGCCAG GAGAAGCTAA TCTGGAGGAC 
CAGCTGGACT CCGGGTCCCA GTGCATCCCC CAGGCAGTGG GCAGGAGGCC CCTGCGGGAC 
TTCGTAGGGC TGGAGGACTG CGACAAGCCC ACGCGGGACG CCATGATCAA CTTCAGCTTT 
TTCCTGACCA TCGGGGACAT GGATGAGGCC TTCAGGTCCA TCAAGCTCAT CAAAAGCCAG 
GCCGTCTGGG AGAACATGGC ACGCATGTGC GTGAAGACTC AGCGGCTGGA CGTGGCCAGG 
GTGTGCCTGG GGCACATGGG CCACGCCCGG GGGGCCCGAG CGCTGCGCGA GGCCCAGCGG 
GAGCCGGAGC CGGAGGCCAG GGTGGCCATG CTGGCCGTGC AGTTGGGCAT GCTGGAGGAC 
GCAGAGCAGC TCTACAGGAG GTGTGGGCGC TACGACCTCC TCAACCGCCT GCTGCAGGCC 
TCAGGCCAGT GGCAGAGGGC CATCGAGGTG GCCGAGCGCC ACGACCGCAT GCACCTGCGC 
ACCACCTACT ACAACTACGC CCGGCACCTG GAGGCCAGCG GGGACTGCAG CCCGGCCCTC 
AGCTACTACG AGAAGTCGGA CACGCACCGC TTCGAGGTGC CGCGGATGCT GGTGGAGGAC 
CTGCAGGCTC TGGGGCTGTA CGTCAACAAG ATGAAGGACA AGACGCTGTG GCGGTGGTGG 
GCCCAGTACC TGGAGAGCCA GGCCAAGATG GACGCGGCGC TGCACTACTA CGAGCTGGCC 
CAGGACTACT TCTCCCTGGT CCGCATCCAC TGCTTCCAGG GCAACATCCA GCGGGCCGCC 
GAGATAGCCA GCGAGACTGG GAACTGGGCG GCCTCCTACC ACCTGGCCCG GCAGTACGAG 
AGCCAGAACG AGGTGCGGCA GGCCGTGCAC TTCTACACGC GTGCCCAGGC CTTCAACAAC 
GCCATCCGCC TGTGCAAGGA GCACGGCCTG GACGACCAGC TCATGAACCT GGCCCTGCTG 
AGCTCCCCCG AGGACATGCT GGAGGCCGCG CGCTACTACG AGGAGAGGGG CGAGCAGATG 
GACCGGGCCG TCATGCTGTA CCACAAGGCC GGCCACTTCT CCAAGGCCCT GGAGCTGGCC 
TTTGCCACCC AGCAGTTTGC AGCCCTGCAG CTGGTGGCAG AGGACCTGGA TGAAAAGTCG 
GATCCCGTCC TCCTGGCCCG TTGCTCTGAC TTCTTCCTGG AGCACGGTCA GTGCGAGAAG 
GCCGTGGAGC TGCTGCTGGC CGCCAAGAAG TATCAGGAAG CACTGCAGCT GTGCCTGGAG 
CAGAACCTGA CCATCACCGA GGAGATGGCT GAGAAGATGA CGGTGCCCAA GGACTGCAAG 
GAGCTGTCCG AGGAGGCCCG GCGGGTGCTG CTGGAGCAGA TCGCCAGCTG CTGCATGCGC 
CAGGGCAGCT ACCACCTGGC CACCAAGAAG TACACGCAGG CAGGGGACAG GCTGAAGGCC 
ATGAGGGCGC TGCTCAAGTC TGGGGACACG GAGAAGATCG TGTTCTTCGC GGGCGTCTCG 
CGGCAGAAGG AGATCTATGT CATGGCGGCT AACTACCTGC AGTCCCTGGA CTGGCGGAAG 
GACCCCGAGG TCATGAAGAG CATCATCAGC TTCTACACCA AGGGCCGTGC GCTGGACCTT 
CTGGCCGGCT TCTACGATGC CTGCGCCCAG GTGGAGGTCG ACGAGTACCA GAACTACGAC 
AAGGCACATG GGGCGCTGAC CGAGGCCTAC AAGTGCCTGT CCAAGGCCAA GGCCAGGAGC 
CCACTGGACC AGGAGGCCAG GCTGGCGCAG CTGCAGAGCA GGATGACGCT GGTGAAGCGC 
TTCATGCAGG CGCGCAGGAC CTACACGGAG GACCCCAAGG GGTCGGTCCG GCAGTGCCAG 
CTGCTCCTGG AGGAGCCGCA CCTGGACAGC GGCGTCCGTG TCGGGGACGT CTACGGCTTC 
CTGGTGGAGC GCCACGTGCA CTCGGGAGAC CTGCAGACGG CCTACAGGTA CCTGGAGGAG 
ATGCGGCAGC GGCTGCCCTC CGCCAACGTG TCCTACTACG TGAGCCAGCA CACCGTGGAC 
GCCGTGCACC AGGGCCTGGG GCTCCCTCTG ACCTGCGCAG CGCCGAAGCG CGCGCAGCAC 
AGCAGCATGG AGGAGCACAA GGAGGCGGAC GAGGAGGTGG CCGAGGAGGT GGAAAGTGGG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324735.1
CDS	392..4717
Translation	MALYFDHRIEAPDTVGSPSHIGWHPIYPFLAVASVSTTSGGSVDVYLEQGERVPDMHVERSFRVTCLCWHPTRLILAIGWESGEVVMFNKQDKEQHAVPATHTADIAILAWSPNGNCLVSGDRLGVLLLWRLDQRGRVQGMPLLRHEYGQPLTHCIFLLPPPGEDLVQLARAAVSGDEKALDMFNWKKSGFRSILKMGSQEGLSFFVSLTDGTVHHVDEKGRTAQVASTDSSIQTLFYLEQRGALVAVTDSLLLCLFTVTPEGTAEEVMKVKLSGKTGHRADIALIDGSLLVTAIGEAALRFWDLERGENYVLSPEEKFGFEKGENINCVSYCKVKGLLAAGTDKGRVAMWRKAPGLPNGRGAEGKDKWTLQAPTELEGNIMQIKWGSRKSLLAVNSISSVLILHEQAMSSHFHQQVAAVQVSPSLLSVSFLSTGVTHSLRTDMHVSGVFATKDAVAVWNGKQVVIFEPSGATLRSAGSFLCESPVLAMHEENVYTVEPNRVQVRTWQGTVKQLLPFSESEGNPCFLDICGTFLVVGTDLAHFKSFDLSRREAKVHCGCKSLAELVPGVGGLTSLRCSASGSKISLLLSKADNSPDSKICFYDVDMDTVTILDLKTGQIDQRETLSINGQETKKSQALEDERLTDLVPVSHFWDQSEPRLFVCEAVREADVVVLSFFVSEEHGFLLQDSFPRPPAFQTLLGIQVPHYYFTRKPGEANLEDQLDSGSQCIPQAVGRRPLRDFVGLEDCDKPTRDAMINFSFFLTIGDMDEAFRSIKLIKSQAVWENMARMCVKTQRLDVARVCLGHMGHARGARALREAQREPEPEARVAMLAVQLGMLEDAEQLYRRCGRYDLLNRLLQASGQWQRAIEVAERHDRMHLRTTYYNYARHLEASGDCSPALSYYEKSDTHRFEVPRMLVEDLQALGLYVNKMKDKTLWRWWAQYLESQAKMDAALHYYELAQDYFSLVRIHCFQGNIQRAAEIASETGNWAASYHLARQYESQNEVRQAVHFYTRAQAFNNAIRLCKEHGLDDQLMNLALLSSPEDMLEAARYYEERGEQMDRAVMLYHKAGHFSKALELAFATQQFAALQLVAEDLDEKSDPVLLARCSDFFLEHGQCEKAVELLLAAKKYQEALQLCLEQNLTITEEMAEKMTVPKDCKELSEEARRVLLEQIASCCMRQGSYHLATKKYTQAGDRLKAMRALLKSGDTEKIVFFAGVSRQKEIYVMAANYLQSLDWRKDPEVMKSIISFYTKGRALDLLAGFYDACAQVEVDEYQNYDKAHGALTEAYKCLSKAKARSPLDQEARLAQLQSRMTLVKRFMQARRTYTEDPKGSVRQCQLLLEEPHLDSGVRVGDVYGFLVERHVHSGDLQTAYRYLEEMRQRLPSANVSYYVSQHTVDAVHQGLGLPLTCAAPKRAQHSSMEEHKEADEEVAEEVESGP

Target ORF information:

RefSeq Version	XM_015469249.1
Organism	Bos taurus(cattle)
Definition	Bos taurus intraflagellar transport 140 (IFT140), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469249.1

ORF Insert Sequence:

ATGGCCCTCT ACTTTGACCA CCGCATAGAA GCCCCAGACA CAGTCGGGTC TCCGTCCCAC 
ATTGGCTGGC ATCCCATCTA CCCATTCTTG GCGGTTGCTT CTGTTAGCAC AACCTCAGGA 
GGCAGCGTGG ATGTGTATCT GGAGCAAGGC GAGCGCGTGC CAGACATGCA TGTTGAGAGG 
TCGTTCCGGG TCACCTGCCT GTGCTGGCAC CCAACGCGGC TGATCCTGGC TATTGGCTGG 
GAGTCTGGAG AAGTGGTCAT GTTTAACAAG CAGGACAAGG AGCAGCATGC AGTCCCCGCC 
ACGCACACAG CCGACATTGC CATCCTCGCC TGGAGCCCCA ACGGGAACTG CCTAGTGTCG 
GGGGACAGGC TTGGCGTCCT GCTCCTCTGG AGGCTGGACC AGCGGGGCCG CGTGCAGGGG 
ATGCCGCTAC TGCGGCACGA GTACGGGCAG CCCCTCACCC ACTGCATCTT CCTGCTGCCC 
CCGCCTGGCG AGGACCTGGT TCAGCTGGCC AGGGCGGCAG TGAGCGGGGA CGAGAAGGCC 
CTGGACATGT TTAACTGGAA GAAGAGCGGC TTCAGGAGCA TCCTGAAGAT GGGGTCTCAG 
GAGGGCCTCT CGTTCTTTGT CAGCCTGACA GACGGGACCG TGCACCACGT GGACGAGAAA 
GGCAGGACGG CCCAGGTGGC ATCCACAGAC AGCTCCATCC AGACACTGTT CTACCTGGAG 
CAGAGGGGGG CGCTGGTGGC GGTGACGGAC AGCCTGCTGC TCTGCCTGTT CACAGTGACG 
CCCGAGGGCA CGGCCGAGGA GGTGATGAAG GTGAAGCTGA GCGGGAAGAC GGGCCACCGG 
GCAGACATCG CGCTGATCGA CGGCAGCCTG CTCGTGACCG CCATCGGAGA GGCGGCGCTC 
AGATTCTGGG ATTTAGAACG AGGGGAGAAC TACGTCCTGA GTCCAGAGGA GAAGTTTGGT 
TTTGAAAAAG GAGAGAATAT AAATTGCGTT TCTTACTGTA AAGTTAAAGG TCTCCTGGCA 
GCAGGTACCG ACAAGGGGCG AGTAGCCATG TGGAGAAAAG CGCCGGGCCT CCCGAACGGC 
CGCGGGGCGG AGGGCAAAGA CAAGTGGACC CTTCAGGCCC CCACTGAGCT GGAAGGAAAC 
ATCATGCAGA TAAAGTGGGG CTCCAGGAAG AGCCTCCTGG CCGTGAACAG CATCAGCTCC 
GTGCTCATCC TCCATGAACA GGCCATGTCA TCCCACTTCC ACCAGCAGGT GGCCGCCGTG 
CAGGTCTCCC CAAGCCTCCT CAGCGTGTCC TTCCTGTCCA CGGGGGTGAC CCACAGCCTG 
CGCACAGACA TGCATGTCAG CGGAGTGTTT GCCACCAAGG ATGCTGTTGC CGTCTGGAAT 
GGAAAACAGG TGGTGATCTT TGAGCCGTCT GGAGCCACGT TACGCAGTGC AGGGTCCTTC 
CTGTGTGAGT CTCCAGTGTT AGCCATGCAT GAAGAAAACG TTTATACGGT GGAGCCAAAC 
AGAGTCCAAG TTCGAACCTG GCAGGGGACC GTGAAGCAGC TCCTGCCGTT CTCCGAGTCA 
GAGGGGAACC CATGCTTCTT GGACATCTGT GGAACTTTCC TGGTTGTAGG GACGGACTTG 
GCTCACTTTA AAAGCTTTGA TCTTTCCCGA AGAGAGGCCA AGGTGCACTG CGGCTGCAAG 
AGCCTGGCCG AGCTGGTCCC AGGCGTGGGG GGCCTGACCT CACTCAGATG CAGTGCCAGC 
GGCAGCAAGA TCAGTCTCCT CCTCAGCAAG GCTGACAATA GCCCCGATTC AAAAATCTGC 
TTCTACGACG TGGATATGGA CACAGTGACC ATCCTCGACC TCAAGACAGG ACAGATAGAT 
CAGAGAGAGA CATTGTCCAT TAATGGGCAG GAGACAAAGA AGAGCCAGGC CCTGGAGGAT 
GAGAGGCTGA CAGACCTCGT GCCCGTCAGC CACTTCTGGG ATCAGAGCGA GCCCCGGCTG 
TTCGTGTGCG AAGCCGTGCG CGAGGCAGAC GTGGTGGTCC TGTCCTTCTT CGTCTCAGAA 
GAGCACGGCT TCCTGTTGCA GGACAGCTTC CCCCGTCCCC CTGCCTTCCA GACGCTCCTG 
GGGATCCAGG TGCCCCACTA CTACTTCACG AGAAAGCCAG GAGAAGCTAA TCTGGAGGAC 
CAGCTGGACT CCGGGTCCCA GTGCATCCCC CAGGCAGTGG GCAGGAGGCC CCTGCGGGAC 
TTCGTAGGGC TGGAGGACTG CGACAAGCCC ACGCGGGACG CCATGATCAA CTTCAGCTTT 
TTCCTGACCA TCGGGGACAT GGATGAGGCC TTCAGGTCCA TCAAGCTCAT CAAAAGCCAG 
GCCGTCTGGG AGAACATGGC ACGCATGTGC GTGAAGACTC AGCGGCTGGA CGTGGCCAGG 
GTGTGCCTGG GGCACATGGG CCACGCCCGG GGGGCCCGAG CGCTGCGCGA GGCCCAGCGG 
GAGCCGGAGC CGGAGGCCAG GGTGGCCATG CTGGCCGTGC AGTTGGGCAT GCTGGAGGAC 
GCAGAGCAGC TCTACAGGAG GTGTGGGCGC TACGACCTCC TCAACCGCCT GCTGCAGGCC 
TCAGGCCAGT GGCAGAGGGC CATCGAGGTG GCCGAGCGCC ACGACCGCAT GCACCTGCGC 
ACCACCTACT ACAACTACGC CCGGCACCTG GAGGCCAGCG GGGACTGCAG CCCGGCCCTC 
AGCTACTACG AGAAGTCGGA CACGCACCGC TTCGAGGTGC CGCGGATGCT GGTGGAGGAC 
CTGCAGGCTC TGGGGCTGTA CGTCAACAAG ATGAAGGACA AGACGCTGTG GCGGTGGTGG 
GCCCAGTACC TGGAGAGCCA GGCCAAGATG GACGCGGCGC TGCACTACTA CGAGCTGGCC 
CAGGACTACT TCTCCCTGGT CCGCATCCAC TGCTTCCAGG GCAACATCCA GCGGGCCGCC 
GAGATAGCCA GCGAGACTGG GAACTGGGCG GCCTCCTACC ACCTGGCCCG GCAGTACGAG 
AGCCAGAACG AGGTGCGGCA GGCCGTGCAC TTCTACACGC GTGCCCAGGC CTTCAACAAC 
GCCATCCGCC TGTGCAAGGA GCACGGCCTG GACGACCAGC TCATGAACCT GGCCCTGCTG 
AGCTCCCCCG AGGACATGCT GGAGGCCGCG CGCTACTACG AGGAGAGGGG CGAGCAGATG 
GACCGGGCCG TCATGCTGTA CCACAAGGCC GGCCACTTCT CCAAGGCCCT GGAGCTGGCC 
TTTGCCACCC AGCAGTTTGC AGCCCTGCAG CTGGTGGCAG AGGACCTGGA TGAAAAGTCG 
GATCCCGTCC TCCTGGCCCG TTGCTCTGAC TTCTTCCTGG AGCACGGTCA GTGCGAGAAG 
GCCGTGGAGC TGCTGCTGGC CGCCAAGAAG TATCAGGAAG CACTGCAGCT GTGCCTGGAG 
CAGAACCTGA CCATCACCGA GGAGATGGCT GAGAAGATGA CGGTGCCCAA GGACTGCAAG 
GAGCTGTCCG AGGAGGCCCG GCGGGTGCTG CTGGAGCAGA TCGCCAGCTG CTGCATGCGC 
CAGGGCAGCT ACCACCTGGC CACCAAGAAG TACACGCAGG CAGGGGACAG GCTGAAGGCC 
ATGAGGGCGC TGCTCAAGTC TGGGGACACG GAGAAGATCG TGTTCTTCGC GGGCGTCTCG 
CGGCAGAAGG AGATCTATGT CATGGCGGCT AACTACCTGC AGTCCCTGGA CTGGCGGAAG 
GACCCCGAGG TCATGAAGAG CATCATCAGC TTCTACACCA AGGGCCGTGC GCTGGACCTT 
CTGGCCGGCT TCTACGATGC CTGCGCCCAG GTGGAGGTCG ACGAGTACCA GAACTACGAC 
AAGGCACATG GGGCGCTGAC CGAGGCCTAC AAGTGCCTGT CCAAGGCCAA GGCCAGGAGC 
CCACTGGACC AGGAGGCCAG GCTGGCGCAG CTGCAGAGCA GGATGACGCT GGTGAAGCGC 
TTCATGCAGG CGCGCAGGAC CTACACGGAG GACCCCAAGG GGTCGGTCCG GCAGTGCCAG 
CTGCTCCTGG AGGAGCCGCA CCTGGACAGC GGCGTCCGTG TCGGGGACGT CTACGGCTTC 
CTGGTGGAGC GCCACGTGCA CTCGGGAGAC CTGCAGACGG CCTACAGGTA CCTGGAGGAG 
ATGCGGCAGC GGCTGCCCTC CGCCAACGTG TCCTACTACG TGAGCCAGCA CACCGTGGAC 
GCCGTGCACC AGGGCCTGGG GCTCCCTCTG ACCTGCGCAG CGCCGAAGCG CGCGCAGCAC 
AGCAGCATGG AGGAGCACAA GGAGGCGGAC GAGGAGGTGG CCGAGGAGGT GGAAAGTGGG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	IFT140
Entrez Gene ID	100139697
Full Name	intraflagellar transport 140
General protein information	Preferred Names intraflagellar transport 140 Names intraflagellar transport protein 140 homolog intraflagellar transport 140 homolog
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	IFT140	XP_001089057.1
Bos taurus (cattle)	IFT140	XP_002697959.3
Gallus gallus (chicken)	IFT140	NP_001012810.1
Danio rerio (zebrafish)	ift140	XP_695732.4
Mus musculus (house mouse)	Ift140	NP_598887.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000686	XP_311215.4
Pan troglodytes (chimpanzee)	IFT140	XP_003314958.1
Caenorhabditis elegans (roundworm)	che-11	NP_506047.5
Xenopus tropicalis (tropical clawed frog)	ift140	NP_001116497.1
Homo sapiens (human)	IFT140	NP_055529.2
Rattus norvegicus (Norway rat)	Ift140	XP_006246116.1
Drosophila melanogaster (fruit fly)	rempA	NP_995608.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

IFT140 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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