GBA2 cDNA ORF clone, Bos taurus(cattle)

The following GBA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GBA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa05828 XM_002689680.6
Latest version!
Bos taurus glucosylceramidase beta 2 (GBA2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa05828 XM_001790070.6
Latest version!
Bos taurus glucosidase, beta (bile acid) 2 (GBA2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa05829 XM_005199225.3
Latest version!
Bos taurus glucosidase, beta (bile acid) 2 (GBA2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa05829 XM_005210145.4
Latest version!
Bos taurus glucosylceramidase beta 2 (GBA2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa58955 XM_024996204.1
Latest version!
Bos taurus glucosylceramidase beta 2 (GBA2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OBa05829 XM_005210145.3 Bos taurus glucosidase, beta (bile acid) 2 (GBA2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa05828 XM_002689680.5 Bos taurus glucosidase, beta (bile acid) 2 (GBA2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa05828
    Clone ID Related Accession (Same CDS sequence) XM_002689680.5 , XM_001790070.6 , XM_002689680.6
    Accession Version XM_002689680.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product non-lysosomal glucosylceramidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002689680.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGTCTATCGG
    GACTATTACC TGACGGGTGA CCAATGTTTC TTGAGGGACA TGTGGCCTGT GTGTCTGGCC
    GTGATGGAGT CTGAAATGAA GTTTGACAAG GACCACGATG GACTCATCGA GAATGGAGGC
    TATGCAGACC AAACCTATGA TGGATGGGTC ACCACAGGCC CCAGTGCTTA CTGCGGAGGA
    CTGTGGCTGG CGGCCGTGGC TGTGATGGTC CAGATGGCTG CTCTGTGTGG GGCACAGGAG
    GCCCAGAATA AGTTTTCTTC CATCCTTAGC CGGGGCCAGG AAGCCTATGA TCGGCTGCTG
    TGGAATGGCC GCTACTACAA CTACGACTGC AGCTCTCAGC CTCAGTCCTG TAGCATCATG
    TCTGACCAGT GTGCTGGCCA GTGGTTCCTA AGGGCCAGTG GCCTAGGAGA AGGAGACAGT
    GAGGTCTTTC CTACCCAACA GGTGGTCCGT GCTCTCCAAA CGATCTTTGA GTTCAATGTC
    CAGGCCTTTG CAGGAGGGGC CATGGGGGCT GTGAATGGCA TGCAGCCCTG TGGTGTTCCC
    GACACATCCA GCGTCCAGTC TGATGAAGTC TGGGTGGGCG TGGTCTACGG GCTGGCAGCC
    ACCATGATCC AAGAGGGCCT GACTCGGGAG GGCTTCCGGA CAGCTGAAGG CTGCTATCGT
    ACTGTGTGGG AGCGCCTGGG CCTGGCCTTC CAGACTCCTG AGGCGTACTG CCAGCGGCGG
    GTGTTCCGCT CACTGGCCTA CATGCGGCCA CTGAGCATCT GGGCCATGCA GCTGGCCCTG
    CAGCAGCAGC AGCAACATAA AAAGGCCTCT GGGCCAATAT CCAAACGGGG CACAGGACTA
    TGCACCAGGC CCAAACGTGG ACCAAAGGAA GCCATGGCAA ACCCAAGCCC AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689726.1
    CDS405..3161
    Translation

    Target ORF information:

    RefSeq Version XM_002689680.5
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase, beta (bile acid) 2 (GBA2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002689680.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGTCTATCGG
    GACTATTACC TGACGGGTGA CCAATGTTTC TTGAGGGACA TGTGGCCTGT GTGTCTGGCC
    GTGATGGAGT CTGAAATGAA GTTTGACAAG GACCACGATG GACTCATCGA GAATGGAGGC
    TATGCAGACC AAACCTATGA TGGATGGGTC ACCACAGGCC CCAGTGCTTA CTGCGGAGGA
    CTGTGGCTGG CGGCCGTGGC TGTGATGGTC CAGATGGCTG CTCTGTGTGG GGCACAGGAG
    GCCCAGAATA AGTTTTCTTC CATCCTTAGC CGGGGCCAGG AAGCCTATGA TCGGCTGCTG
    TGGAATGGCC GCTACTACAA CTACGACTGC AGCTCTCAGC CTCAGTCCTG TAGCATCATG
    TCTGACCAGT GTGCTGGCCA GTGGTTCCTA AGGGCCAGTG GCCTAGGAGA AGGAGACAGT
    GAGGTCTTTC CTACCCAACA GGTGGTCCGT GCTCTCCAAA CGATCTTTGA GTTCAATGTC
    CAGGCCTTTG CAGGAGGGGC CATGGGGGCT GTGAATGGCA TGCAGCCCTG TGGTGTTCCC
    GACACATCCA GCGTCCAGTC TGATGAAGTC TGGGTGGGCG TGGTCTACGG GCTGGCAGCC
    ACCATGATCC AAGAGGGCCT GACTCGGGAG GGCTTCCGGA CAGCTGAAGG CTGCTATCGT
    ACTGTGTGGG AGCGCCTGGG CCTGGCCTTC CAGACTCCTG AGGCGTACTG CCAGCGGCGG
    GTGTTCCGCT CACTGGCCTA CATGCGGCCA CTGAGCATCT GGGCCATGCA GCTGGCCCTG
    CAGCAGCAGC AGCAACATAA AAAGGCCTCT GGGCCAATAT CCAAACGGGG CACAGGACTA
    TGCACCAGGC CCAAACGTGG ACCAAAGGAA GCCATGGCAA ACCCAAGCCC AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05828
    Clone ID Related Accession (Same CDS sequence) XM_002689680.5 , XM_001790070.6 , XM_002689680.6
    Accession Version XM_001790070.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product non-lysosomal glucosylceramidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645279.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001790070.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGTCTATCGG
    GACTATTACC TGACGGGTGA CCAATGTTTC TTGAGGGACA TGTGGCCTGT GTGTCTGGCC
    GTGATGGAGT CTGAAATGAA GTTTGACAAG GACCACGATG GACTCATCGA GAATGGAGGC
    TATGCAGACC AAACCTATGA TGGATGGGTC ACCACAGGCC CCAGTGCTTA CTGCGGAGGA
    CTGTGGCTGG CGGCCGTGGC TGTGATGGTC CAGATGGCTG CTCTGTGTGG GGCACAGGAG
    GCCCAGAATA AGTTTTCTTC CATCCTTAGC CGGGGCCAGG AAGCCTATGA TCGGCTGCTG
    TGGAATGGCC GCTACTACAA CTACGACTGC AGCTCTCAGC CTCAGTCCTG TAGCATCATG
    TCTGACCAGT GTGCTGGCCA GTGGTTCCTA AGGGCCAGTG GCCTAGGAGA AGGAGACAGT
    GAGGTCTTTC CTACCCAACA GGTGGTCCGT GCTCTCCAAA CGATCTTTGA GTTCAATGTC
    CAGGCCTTTG CAGGAGGGGC CATGGGGGCT GTGAATGGCA TGCAGCCCTG TGGTGTTCCC
    GACACATCCA GCGTCCAGTC TGATGAAGTC TGGGTGGGCG TGGTCTACGG GCTGGCAGCC
    ACCATGATCC AAGAGGGCCT GACTCGGGAG GGCTTCCGGA CAGCTGAAGG CTGCTATCGT
    ACTGTGTGGG AGCGCCTGGG CCTGGCCTTC CAGACTCCTG AGGCGTACTG CCAGCGGCGG
    GTGTTCCGCT CACTGGCCTA CATGCGGCCA CTGAGCATCT GGGCCATGCA GCTGGCCCTG
    CAGCAGCAGC AGCAACATAA AAAGGCCTCT GGGCCAATAT CCAAACGGGG CACAGGACTA
    TGCACCAGGC CCAAACGTGG ACCAAAGGAA GCCATGGCAA ACCCAAGCCC AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001790122.1
    CDS405..3161
    Translation

    Target ORF information:

    RefSeq Version XM_001790070.6
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase, beta (bile acid) 2 (GBA2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001790070.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGTCTATCGG
    GACTATTACC TGACGGGTGA CCAATGTTTC TTGAGGGACA TGTGGCCTGT GTGTCTGGCC
    GTGATGGAGT CTGAAATGAA GTTTGACAAG GACCACGATG GACTCATCGA GAATGGAGGC
    TATGCAGACC AAACCTATGA TGGATGGGTC ACCACAGGCC CCAGTGCTTA CTGCGGAGGA
    CTGTGGCTGG CGGCCGTGGC TGTGATGGTC CAGATGGCTG CTCTGTGTGG GGCACAGGAG
    GCCCAGAATA AGTTTTCTTC CATCCTTAGC CGGGGCCAGG AAGCCTATGA TCGGCTGCTG
    TGGAATGGCC GCTACTACAA CTACGACTGC AGCTCTCAGC CTCAGTCCTG TAGCATCATG
    TCTGACCAGT GTGCTGGCCA GTGGTTCCTA AGGGCCAGTG GCCTAGGAGA AGGAGACAGT
    GAGGTCTTTC CTACCCAACA GGTGGTCCGT GCTCTCCAAA CGATCTTTGA GTTCAATGTC
    CAGGCCTTTG CAGGAGGGGC CATGGGGGCT GTGAATGGCA TGCAGCCCTG TGGTGTTCCC
    GACACATCCA GCGTCCAGTC TGATGAAGTC TGGGTGGGCG TGGTCTACGG GCTGGCAGCC
    ACCATGATCC AAGAGGGCCT GACTCGGGAG GGCTTCCGGA CAGCTGAAGG CTGCTATCGT
    ACTGTGTGGG AGCGCCTGGG CCTGGCCTTC CAGACTCCTG AGGCGTACTG CCAGCGGCGG
    GTGTTCCGCT CACTGGCCTA CATGCGGCCA CTGAGCATCT GGGCCATGCA GCTGGCCCTG
    CAGCAGCAGC AGCAACATAA AAAGGCCTCT GGGCCAATAT CCAAACGGGG CACAGGACTA
    TGCACCAGGC CCAAACGTGG ACCAAAGGAA GCCATGGCAA ACCCAAGCCC AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05828
    Clone ID Related Accession (Same CDS sequence) XM_002689680.5 , XM_001790070.6 , XM_002689680.6
    Accession Version XM_002689680.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product non-lysosomal glucosylceramidase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002689680.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGTCTATCGG
    GACTATTACC TGACGGGTGA CCAATGTTTC TTGAGGGACA TGTGGCCTGT GTGTCTGGCC
    GTGATGGAGT CTGAAATGAA GTTTGACAAG GACCACGATG GACTCATCGA GAATGGAGGC
    TATGCAGACC AAACCTATGA TGGATGGGTC ACCACAGGCC CCAGTGCTTA CTGCGGAGGA
    CTGTGGCTGG CGGCCGTGGC TGTGATGGTC CAGATGGCTG CTCTGTGTGG GGCACAGGAG
    GCCCAGAATA AGTTTTCTTC CATCCTTAGC CGGGGCCAGG AAGCCTATGA TCGGCTGCTG
    TGGAATGGCC GCTACTACAA CTACGACTGC AGCTCTCAGC CTCAGTCCTG TAGCATCATG
    TCTGACCAGT GTGCTGGCCA GTGGTTCCTA AGGGCCAGTG GCCTAGGAGA AGGAGACAGT
    GAGGTCTTTC CTACCCAACA GGTGGTCCGT GCTCTCCAAA CGATCTTTGA GTTCAATGTC
    CAGGCCTTTG CAGGAGGGGC CATGGGGGCT GTGAATGGCA TGCAGCCCTG TGGTGTTCCC
    GACACATCCA GCGTCCAGTC TGATGAAGTC TGGGTGGGCG TGGTCTACGG GCTGGCAGCC
    ACCATGATCC AAGAGGGCCT GACTCGGGAG GGCTTCCGGA CAGCTGAAGG CTGCTATCGT
    ACTGTGTGGG AGCGCCTGGG CCTGGCCTTC CAGACTCCTG AGGCGTACTG CCAGCGGCGG
    GTGTTCCGCT CACTGGCCTA CATGCGGCCA CTGAGCATCT GGGCCATGCA GCTGGCCCTG
    CAGCAGCAGC AGCAACATAA AAAGGCCTCT GGGCCAATAT CCAAACGGGG CACAGGACTA
    TGCACCAGGC CCAAACGTGG ACCAAAGGAA GCCATGGCAA ACCCAAGCCC AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689726.1
    CDS263..3019
    Translation

    Target ORF information:

    RefSeq Version XM_002689680.6
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosylceramidase beta 2 (GBA2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002689680.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGTCTATCGG
    GACTATTACC TGACGGGTGA CCAATGTTTC TTGAGGGACA TGTGGCCTGT GTGTCTGGCC
    GTGATGGAGT CTGAAATGAA GTTTGACAAG GACCACGATG GACTCATCGA GAATGGAGGC
    TATGCAGACC AAACCTATGA TGGATGGGTC ACCACAGGCC CCAGTGCTTA CTGCGGAGGA
    CTGTGGCTGG CGGCCGTGGC TGTGATGGTC CAGATGGCTG CTCTGTGTGG GGCACAGGAG
    GCCCAGAATA AGTTTTCTTC CATCCTTAGC CGGGGCCAGG AAGCCTATGA TCGGCTGCTG
    TGGAATGGCC GCTACTACAA CTACGACTGC AGCTCTCAGC CTCAGTCCTG TAGCATCATG
    TCTGACCAGT GTGCTGGCCA GTGGTTCCTA AGGGCCAGTG GCCTAGGAGA AGGAGACAGT
    GAGGTCTTTC CTACCCAACA GGTGGTCCGT GCTCTCCAAA CGATCTTTGA GTTCAATGTC
    CAGGCCTTTG CAGGAGGGGC CATGGGGGCT GTGAATGGCA TGCAGCCCTG TGGTGTTCCC
    GACACATCCA GCGTCCAGTC TGATGAAGTC TGGGTGGGCG TGGTCTACGG GCTGGCAGCC
    ACCATGATCC AAGAGGGCCT GACTCGGGAG GGCTTCCGGA CAGCTGAAGG CTGCTATCGT
    ACTGTGTGGG AGCGCCTGGG CCTGGCCTTC CAGACTCCTG AGGCGTACTG CCAGCGGCGG
    GTGTTCCGCT CACTGGCCTA CATGCGGCCA CTGAGCATCT GGGCCATGCA GCTGGCCCTG
    CAGCAGCAGC AGCAACATAA AAAGGCCTCT GGGCCAATAT CCAAACGGGG CACAGGACTA
    TGCACCAGGC CCAAACGTGG ACCAAAGGAA GCCATGGCAA ACCCAAGCCC AGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05829
    Clone ID Related Accession (Same CDS sequence) XM_005210145.3 , XM_005210145.4 , XM_005199225.3
    Accession Version XM_005210145.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product non-lysosomal glucosylceramidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210145.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGCCGTGATG
    GAGTCTGAAA TGAAGTTTGA CAAGGACCAC GATGGACTCA TCGAGAATGG AGGCTATGCA
    GACCAAACCT ATGATGGATG GGTCACCACA GGCCCCAGTG CTTACTGCGG AGGACTGTGG
    CTGGCGGCCG TGGCTGTGAT GGTCCAGATG GCTGCTCTGT GTGGGGCACA GGAGGCCCAG
    AATAAGTTTT CTTCCATCCT TAGCCGGGGC CAGGAAGCCT ATGATCGGCT GCTGTGGAAT
    GGCCGCTACT ACAACTACGA CTGCAGCTCT CAGCCTCAGT CCTGTAGCAT CATGTCTGAC
    CAGTGTGCTG GCCAGTGGTT CCTAAGGGCC AGTGGCCTAG GAGAAGGAGA CAGTGAGGTC
    TTTCCTACCC AACAGGTGGT CCGTGCTCTC CAAACGATCT TTGAGTTCAA TGTCCAGGCC
    TTTGCAGGAG GGGCCATGGG GGCTGTGAAT GGCATGCAGC CCTGTGGTGT TCCCGACACA
    TCCAGCGTCC AGTCTGATGA AGTCTGGGTG GGCGTGGTCT ACGGGCTGGC AGCCACCATG
    ATCCAAGAGG GCCTGACTCG GGAGGGCTTC CGGACAGCTG AAGGCTGCTA TCGTACTGTG
    TGGGAGCGCC TGGGCCTGGC CTTCCAGACT CCTGAGGCGT ACTGCCAGCG GCGGGTGTTC
    CGCTCACTGG CCTACATGCG GCCACTGAGC ATCTGGGCCA TGCAGCTGGC CCTGCAGCAG
    CAGCAGCAAC ATAAAAAGGC CTCTGGGCCA ATATCCAAAC GGGGCACAGG ACTATGCACC
    AGGCCCAAAC GTGGACCAAA GGAAGCCATG GCAAACCCAA GCCCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210202.1
    CDS405..3095
    Translation

    Target ORF information:

    RefSeq Version XM_005210145.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase, beta (bile acid) 2 (GBA2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210145.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGCCGTGATG
    GAGTCTGAAA TGAAGTTTGA CAAGGACCAC GATGGACTCA TCGAGAATGG AGGCTATGCA
    GACCAAACCT ATGATGGATG GGTCACCACA GGCCCCAGTG CTTACTGCGG AGGACTGTGG
    CTGGCGGCCG TGGCTGTGAT GGTCCAGATG GCTGCTCTGT GTGGGGCACA GGAGGCCCAG
    AATAAGTTTT CTTCCATCCT TAGCCGGGGC CAGGAAGCCT ATGATCGGCT GCTGTGGAAT
    GGCCGCTACT ACAACTACGA CTGCAGCTCT CAGCCTCAGT CCTGTAGCAT CATGTCTGAC
    CAGTGTGCTG GCCAGTGGTT CCTAAGGGCC AGTGGCCTAG GAGAAGGAGA CAGTGAGGTC
    TTTCCTACCC AACAGGTGGT CCGTGCTCTC CAAACGATCT TTGAGTTCAA TGTCCAGGCC
    TTTGCAGGAG GGGCCATGGG GGCTGTGAAT GGCATGCAGC CCTGTGGTGT TCCCGACACA
    TCCAGCGTCC AGTCTGATGA AGTCTGGGTG GGCGTGGTCT ACGGGCTGGC AGCCACCATG
    ATCCAAGAGG GCCTGACTCG GGAGGGCTTC CGGACAGCTG AAGGCTGCTA TCGTACTGTG
    TGGGAGCGCC TGGGCCTGGC CTTCCAGACT CCTGAGGCGT ACTGCCAGCG GCGGGTGTTC
    CGCTCACTGG CCTACATGCG GCCACTGAGC ATCTGGGCCA TGCAGCTGGC CCTGCAGCAG
    CAGCAGCAAC ATAAAAAGGC CTCTGGGCCA ATATCCAAAC GGGGCACAGG ACTATGCACC
    AGGCCCAAAC GTGGACCAAA GGAAGCCATG GCAAACCCAA GCCCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05829
    Clone ID Related Accession (Same CDS sequence) XM_005210145.3 , XM_005210145.4 , XM_005199225.3
    Accession Version XM_005210145.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product non-lysosomal glucosylceramidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210145.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGCCGTGATG
    GAGTCTGAAA TGAAGTTTGA CAAGGACCAC GATGGACTCA TCGAGAATGG AGGCTATGCA
    GACCAAACCT ATGATGGATG GGTCACCACA GGCCCCAGTG CTTACTGCGG AGGACTGTGG
    CTGGCGGCCG TGGCTGTGAT GGTCCAGATG GCTGCTCTGT GTGGGGCACA GGAGGCCCAG
    AATAAGTTTT CTTCCATCCT TAGCCGGGGC CAGGAAGCCT ATGATCGGCT GCTGTGGAAT
    GGCCGCTACT ACAACTACGA CTGCAGCTCT CAGCCTCAGT CCTGTAGCAT CATGTCTGAC
    CAGTGTGCTG GCCAGTGGTT CCTAAGGGCC AGTGGCCTAG GAGAAGGAGA CAGTGAGGTC
    TTTCCTACCC AACAGGTGGT CCGTGCTCTC CAAACGATCT TTGAGTTCAA TGTCCAGGCC
    TTTGCAGGAG GGGCCATGGG GGCTGTGAAT GGCATGCAGC CCTGTGGTGT TCCCGACACA
    TCCAGCGTCC AGTCTGATGA AGTCTGGGTG GGCGTGGTCT ACGGGCTGGC AGCCACCATG
    ATCCAAGAGG GCCTGACTCG GGAGGGCTTC CGGACAGCTG AAGGCTGCTA TCGTACTGTG
    TGGGAGCGCC TGGGCCTGGC CTTCCAGACT CCTGAGGCGT ACTGCCAGCG GCGGGTGTTC
    CGCTCACTGG CCTACATGCG GCCACTGAGC ATCTGGGCCA TGCAGCTGGC CCTGCAGCAG
    CAGCAGCAAC ATAAAAAGGC CTCTGGGCCA ATATCCAAAC GGGGCACAGG ACTATGCACC
    AGGCCCAAAC GTGGACCAAA GGAAGCCATG GCAAACCCAA GCCCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210202.1
    CDS208..2898
    Translation

    Target ORF information:

    RefSeq Version XM_005210145.4
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosylceramidase beta 2 (GBA2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210145.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGCCGTGATG
    GAGTCTGAAA TGAAGTTTGA CAAGGACCAC GATGGACTCA TCGAGAATGG AGGCTATGCA
    GACCAAACCT ATGATGGATG GGTCACCACA GGCCCCAGTG CTTACTGCGG AGGACTGTGG
    CTGGCGGCCG TGGCTGTGAT GGTCCAGATG GCTGCTCTGT GTGGGGCACA GGAGGCCCAG
    AATAAGTTTT CTTCCATCCT TAGCCGGGGC CAGGAAGCCT ATGATCGGCT GCTGTGGAAT
    GGCCGCTACT ACAACTACGA CTGCAGCTCT CAGCCTCAGT CCTGTAGCAT CATGTCTGAC
    CAGTGTGCTG GCCAGTGGTT CCTAAGGGCC AGTGGCCTAG GAGAAGGAGA CAGTGAGGTC
    TTTCCTACCC AACAGGTGGT CCGTGCTCTC CAAACGATCT TTGAGTTCAA TGTCCAGGCC
    TTTGCAGGAG GGGCCATGGG GGCTGTGAAT GGCATGCAGC CCTGTGGTGT TCCCGACACA
    TCCAGCGTCC AGTCTGATGA AGTCTGGGTG GGCGTGGTCT ACGGGCTGGC AGCCACCATG
    ATCCAAGAGG GCCTGACTCG GGAGGGCTTC CGGACAGCTG AAGGCTGCTA TCGTACTGTG
    TGGGAGCGCC TGGGCCTGGC CTTCCAGACT CCTGAGGCGT ACTGCCAGCG GCGGGTGTTC
    CGCTCACTGG CCTACATGCG GCCACTGAGC ATCTGGGCCA TGCAGCTGGC CCTGCAGCAG
    CAGCAGCAAC ATAAAAAGGC CTCTGGGCCA ATATCCAAAC GGGGCACAGG ACTATGCACC
    AGGCCCAAAC GTGGACCAAA GGAAGCCATG GCAAACCCAA GCCCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05829
    Clone ID Related Accession (Same CDS sequence) XM_005210145.3 , XM_005210145.4 , XM_005199225.3
    Accession Version XM_005199225.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product non-lysosomal glucosylceramidase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645279.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199225.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGCCGTGATG
    GAGTCTGAAA TGAAGTTTGA CAAGGACCAC GATGGACTCA TCGAGAATGG AGGCTATGCA
    GACCAAACCT ATGATGGATG GGTCACCACA GGCCCCAGTG CTTACTGCGG AGGACTGTGG
    CTGGCGGCCG TGGCTGTGAT GGTCCAGATG GCTGCTCTGT GTGGGGCACA GGAGGCCCAG
    AATAAGTTTT CTTCCATCCT TAGCCGGGGC CAGGAAGCCT ATGATCGGCT GCTGTGGAAT
    GGCCGCTACT ACAACTACGA CTGCAGCTCT CAGCCTCAGT CCTGTAGCAT CATGTCTGAC
    CAGTGTGCTG GCCAGTGGTT CCTAAGGGCC AGTGGCCTAG GAGAAGGAGA CAGTGAGGTC
    TTTCCTACCC AACAGGTGGT CCGTGCTCTC CAAACGATCT TTGAGTTCAA TGTCCAGGCC
    TTTGCAGGAG GGGCCATGGG GGCTGTGAAT GGCATGCAGC CCTGTGGTGT TCCCGACACA
    TCCAGCGTCC AGTCTGATGA AGTCTGGGTG GGCGTGGTCT ACGGGCTGGC AGCCACCATG
    ATCCAAGAGG GCCTGACTCG GGAGGGCTTC CGGACAGCTG AAGGCTGCTA TCGTACTGTG
    TGGGAGCGCC TGGGCCTGGC CTTCCAGACT CCTGAGGCGT ACTGCCAGCG GCGGGTGTTC
    CGCTCACTGG CCTACATGCG GCCACTGAGC ATCTGGGCCA TGCAGCTGGC CCTGCAGCAG
    CAGCAGCAAC ATAAAAAGGC CTCTGGGCCA ATATCCAAAC GGGGCACAGG ACTATGCACC
    AGGCCCAAAC GTGGACCAAA GGAAGCCATG GCAAACCCAA GCCCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199282.1
    CDS405..3095
    Translation

    Target ORF information:

    RefSeq Version XM_005199225.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosidase, beta (bile acid) 2 (GBA2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199225.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGG TACTTGCAGT GGTGGTACCG TAAGACCCAG
    GTGGAAAAGA AGAAACCTTT CATCGACCTC ATCAACTCTG TGCCCCTGAG ACAGATCTAC
    GGTTGTCCCT TGGGTGGCAT TGGGGGAGGC ACTATCACCA GAGGCTGGAG AGGCCAGTTC
    TGTCGTTGGC AGCTTAATCC TGGAATGTAC CAGCACCAGA CAGTCATCGC TAACCAATTC
    ACAGTGTGTT TGCGCCGGAG AGGACAGACG GTGTACCAGC AAGTCCTGTC GGTGGAGCGC
    CCGAGTGTCC TGTGCAGCTG GAACTGGGGT CTATGTGGGT ACTTTGCATT CTACCACGCC
    CTCTATCCCA GAGCCTGGAC TGTCTATCAG CTTCCTGGCC AGAACGTCAC CCTCACCTGC
    CGCCAGATCA CACCCATCTT ACCCCATGAC TACCAGGACA GCAGCCTGCC TGTAGGAGTC
    TTTGTGTGGG ATGTGGAAAA TGAAGGGGAT GAAGCCCTGG AAGTGTCCAT CATGTTCTCT
    ATGCGGAACG GACTTGGTGG TGAAGACGAC GCCCCCGGGG GGTTGTGGAA TGAGCCCTTC
    TGTCTGGAGC ATGATGGGGA GACTGTTCAG GGGCTGCTGC TGCATCATCC AACCCCCCCG
    AATCCCTACA CCATGGCTGT GGCTGCGCGA CTCTCGGCAG ACACAACGGT AACCCACCTT
    ACAGCCTTTG ACCCTGACAG CGCCGGGCAG CAGGTGTGGC AGGATCTACT TCTGGATGGA
    CAGCTGGACT CCCTCGCTGG AAAAAGTCTC CCTTCGCAGA AAGGAGTGGG CATCGCTGGG
    GCTGTGTGTG TTTCGGGCAA GCTGCCACCA CGAGGCCGGC ACCGCTTAGA GTTCTCACTG
    GCTTGGGACA TGCCCAGAAT CATGTTTGGA GCAAAGGGCC AGGTCTACTA CAGGCGGTAC
    ACAAGGTTCT TTGGCCCAGA CGGTGATGCA GCACCCGCCC TCAGCCACTA TGCCCTGAGG
    CACTATGCCG ACTGGGAAGA GAAGATCTCG GCTTGGCAGA GCCCGGTATT GGAGGACAGA
    TCCTTGCCTG CCTGGTACAA ATCTGCACTG TTCAATGAAC TTTACTTCCT GGCTGACGGG
    GGCACAGTAT GGCTGGAAGT TCCTGAAGAC TCCCTGCCAG AGGAGCTGGT GGGCAGCATG
    GGCCAGCTCC GCCCCCTCCT GCAGGAGTAC GGCCGGTTTG CCTACCTTGA AGGCCAGGAA
    TACCGCATGT ATAACACATA CGATGTCCAC TTTTATGCTT CCTTCGCCCT CATCATGCTC
    TGGCCCAAAC TTGAGCTCAG CCTTCAGTAT GACATGGCTC TGGCCACTCT CAGGGAGGAC
    CTGACACAGC GGCGGTTCCT GATGAGTGGG GTAACAGCGC CTGTGAAGAG GAGGAACGTC
    ATCCCCCATG ATGTTGGGGA CCCAGATGAC GAGCCATGGC TCCGGGTCAA TGCGTACGAG
    ATCCACGATA CTGGAGACTG GAAGGACCTG AACCTGAAGT TTGTGCTGCA GGCCGTGATG
    GAGTCTGAAA TGAAGTTTGA CAAGGACCAC GATGGACTCA TCGAGAATGG AGGCTATGCA
    GACCAAACCT ATGATGGATG GGTCACCACA GGCCCCAGTG CTTACTGCGG AGGACTGTGG
    CTGGCGGCCG TGGCTGTGAT GGTCCAGATG GCTGCTCTGT GTGGGGCACA GGAGGCCCAG
    AATAAGTTTT CTTCCATCCT TAGCCGGGGC CAGGAAGCCT ATGATCGGCT GCTGTGGAAT
    GGCCGCTACT ACAACTACGA CTGCAGCTCT CAGCCTCAGT CCTGTAGCAT CATGTCTGAC
    CAGTGTGCTG GCCAGTGGTT CCTAAGGGCC AGTGGCCTAG GAGAAGGAGA CAGTGAGGTC
    TTTCCTACCC AACAGGTGGT CCGTGCTCTC CAAACGATCT TTGAGTTCAA TGTCCAGGCC
    TTTGCAGGAG GGGCCATGGG GGCTGTGAAT GGCATGCAGC CCTGTGGTGT TCCCGACACA
    TCCAGCGTCC AGTCTGATGA AGTCTGGGTG GGCGTGGTCT ACGGGCTGGC AGCCACCATG
    ATCCAAGAGG GCCTGACTCG GGAGGGCTTC CGGACAGCTG AAGGCTGCTA TCGTACTGTG
    TGGGAGCGCC TGGGCCTGGC CTTCCAGACT CCTGAGGCGT ACTGCCAGCG GCGGGTGTTC
    CGCTCACTGG CCTACATGCG GCCACTGAGC ATCTGGGCCA TGCAGCTGGC CCTGCAGCAG
    CAGCAGCAAC ATAAAAAGGC CTCTGGGCCA ATATCCAAAC GGGGCACAGG ACTATGCACC
    AGGCCCAAAC GTGGACCAAA GGAAGCCATG GCAAACCCAA GCCCAGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58955
    Clone ID Related Accession (Same CDS sequence) XM_024996204.1
    Accession Version XM_024996204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2544bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product non-lysosomal glucosylceramidase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGT TCACAGTGTG TTTGCGCCGG AGAGGACAGA
    CGGTGTACCA GCAAGTCCTG TCGGTGGAGC GCCCGAGTGT CCTGTGCAGC TGGAACTGGG
    GTCTATGTGG GTACTTTGCA TTCTACCACG CCCTCTATCC CAGAGCCTGG ACTGTCTATC
    AGCTTCCTGG CCAGAACGTC ACCCTCACCT GCCGCCAGAT CACACCCATC TTACCCCATG
    ACTACCAGCA GCCTGCCTGT AGGAGTCTTT GTGTGGGATG TGGAAAATGA AGGGGATGAA
    GCCCTGGAAG TGTCCATCAT GTTCTCTATG CGGAACGGAC TTGGTGGTGA AGACGACGCC
    CCCGGGGGGT TGTGGAATGA GCCCTTCTGT CTGGAGCATG ATGGGGAGAC TGTTCAGGGG
    CTGCTGCTGC ATCATCCAAC CCCCCCGAAT CCCTACACCA TGGCTGTGGC TGCGCGACTC
    TCGGCAGACA CAACGGTAAC CCACCTTACA GCCTTTGACC CTGACAGCGC CGGGCAGCAG
    GTGTGGCAGG ATCTACTTCT GGATGGACAG CTGGACTCCC TCGCTGGAAA AAGTCTCCCT
    TCGCAGAAAG GAGTGGGCAT CGCTGGGGCT GTGTGTGTTT CGGGCAAGCT GCCACCACGA
    GGCCGGCACC GCTTAGAGTT CTCACTGGCT TGGGACATGC CCAGAATCAT GTTTGGAGCA
    AAGGGCCAGG TCTACTACAG GCGGTACACA AGGTTCTTTG GCCCAGACGG TGATGCAGCA
    CCCGCCCTCA GCCACTATGC CCTGAGGCAC TATGCCGACT GGGAAGAGAA GATCTCGGCT
    TGGCAGAGCC CGGTATTGGA GGACAGATCC TTGCCTGCCT GGTACAAATC TGCACTGTTC
    AATGAACTTT ACTTCCTGGC TGACGGGGGC ACAGTATGGC TGGAAGTTCC TGAAGACTCC
    CTGCCAGAGG AGCTGGTGGG CAGCATGGGC CAGCTCCGCC CCCTCCTGCA GGAGTACGGC
    CGGTTTGCCT ACCTTGAAGG CCAGGAATAC CGCATGTATA ACACATACGA TGTCCACTTT
    TATGCTTCCT TCGCCCTCAT CATGCTCTGG CCCAAACTTG AGCTCAGCCT TCAGTATGAC
    ATGGCTCTGG CCACTCTCAG GGAGGACCTG ACACAGCGGC GGTTCCTGAT GAGTGGGGTA
    ACAGCGCCTG TGAAGAGGAG GAACGTCATC CCCCATGATG TTGGGGACCC AGATGACGAG
    CCATGGCTCC GGGTCAATGC GTACGAGATC CACGATACTG GAGACTGGAA GGACCTGAAC
    CTGAAGTTTG TGCTGCAGGT CTATCGGGAC TATTACCTGA CGGGTGACCA ATGTTTCTTG
    AGGGACATGT GGCCTGTGTG TCTGGCCGTG ATGGAGTCTG AAATGAAGTT TGACAAGGAC
    CACGATGGAC TCATCGAGAA TGGAGGCTAT GCAGACCAAA CCTATGATGG ATGGGTCACC
    ACAGGCCCCA GTGCTTACTG CGGAGGACTG TGGCTGGCGG CCGTGGCTGT GATGGTCCAG
    ATGGCTGCTC TGTGTGGGGC ACAGGAGGCC CAGAATAAGT TTTCTTCCAT CCTTAGCCGG
    GGCCAGGAAG CCTATGATCG GCTGCTGTGG AATGGCCGCT ACTACAACTA CGACTGCAGC
    TCTCAGCCTC AGTCCTGTAG CATCATGTCT GACCAGTGTG CTGGCCAGTG GTTCCTAAGG
    GCCAGTGGCC TAGGAGAAGG AGACAGTGAG GTCTTTCCTA CCCAACAGGT GGTCCGTGCT
    CTCCAAACGA TCTTTGAGTT CAATGTCCAG GCCTTTGCAG GAGGGGCCAT GGGGGCTGTG
    AATGGCATGC AGCCCTGTGG TGTTCCCGAC ACATCCAGCG TCCAGTCTGA TGAAGTCTGG
    GTGGGCGTGG TCTACGGGCT GGCAGCCACC ATGATCCAAG AGGGCCTGAC TCGGGAGGGC
    TTCCGGACAG CTGAAGGCTG CTATCGTACT GTGTGGGAGC GCCTGGGCCT GGCCTTCCAG
    ACTCCTGAGG CGTACTGCCA GCGGCGGGTG TTCCGCTCAC TGGCCTACAT GCGGCCACTG
    AGCATCTGGG CCATGCAGCT GGCCCTGCAG CAGCAGCAGC AACATAAAAA GGCCTCTGGG
    CCAATATCCA AACGGGGCAC AGGACTATGC ACCAGGCCCA AACGTGGACC AAAGGAAGCC
    ATGGCAAACC CAAGCCCAGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851972.1
    CDS71..2614
    Translation

    Target ORF information:

    RefSeq Version XM_024996204.1
    Organism Bos taurus(cattle)
    Definition Bos taurus glucosylceramidase beta 2 (GBA2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGGAACCG GAGTCCCAGC CTCGGAGCAG ACCAGCTGTG CCAAAGGGGA TCCACAGGCT 
    TCTTGCCCTG AAAATGGCAC GGAGGCTGTG CAGGTTACAG ACTGTCCAAT CCCTGAGGAC
    AGTCCACCCC AGCATGAACA GGGTTACAGC AGTGCGGAGG ACTCCGGGCA GCTGATGGCT
    TCCTACGAGG GGAAAGCTAA GGGCTACCAG GTGCCTCCCT TTGGCTGGCG CATCTGCCTG
    GCTCACGAGT TTGCAGAGAA GAGGAAACCC TTTAATGCCA ATGACATCTC GCTGAGCAAC
    CTGATAAAGC ACTTGGGCAT GGGCTTGAGT TCACAGTGTG TTTGCGCCGG AGAGGACAGA
    CGGTGTACCA GCAAGTCCTG TCGGTGGAGC GCCCGAGTGT CCTGTGCAGC TGGAACTGGG
    GTCTATGTGG GTACTTTGCA TTCTACCACG CCCTCTATCC CAGAGCCTGG ACTGTCTATC
    AGCTTCCTGG CCAGAACGTC ACCCTCACCT GCCGCCAGAT CACACCCATC TTACCCCATG
    ACTACCAGCA GCCTGCCTGT AGGAGTCTTT GTGTGGGATG TGGAAAATGA AGGGGATGAA
    GCCCTGGAAG TGTCCATCAT GTTCTCTATG CGGAACGGAC TTGGTGGTGA AGACGACGCC
    CCCGGGGGGT TGTGGAATGA GCCCTTCTGT CTGGAGCATG ATGGGGAGAC TGTTCAGGGG
    CTGCTGCTGC ATCATCCAAC CCCCCCGAAT CCCTACACCA TGGCTGTGGC TGCGCGACTC
    TCGGCAGACA CAACGGTAAC CCACCTTACA GCCTTTGACC CTGACAGCGC CGGGCAGCAG
    GTGTGGCAGG ATCTACTTCT GGATGGACAG CTGGACTCCC TCGCTGGAAA AAGTCTCCCT
    TCGCAGAAAG GAGTGGGCAT CGCTGGGGCT GTGTGTGTTT CGGGCAAGCT GCCACCACGA
    GGCCGGCACC GCTTAGAGTT CTCACTGGCT TGGGACATGC CCAGAATCAT GTTTGGAGCA
    AAGGGCCAGG TCTACTACAG GCGGTACACA AGGTTCTTTG GCCCAGACGG TGATGCAGCA
    CCCGCCCTCA GCCACTATGC CCTGAGGCAC TATGCCGACT GGGAAGAGAA GATCTCGGCT
    TGGCAGAGCC CGGTATTGGA GGACAGATCC TTGCCTGCCT GGTACAAATC TGCACTGTTC
    AATGAACTTT ACTTCCTGGC TGACGGGGGC ACAGTATGGC TGGAAGTTCC TGAAGACTCC
    CTGCCAGAGG AGCTGGTGGG CAGCATGGGC CAGCTCCGCC CCCTCCTGCA GGAGTACGGC
    CGGTTTGCCT ACCTTGAAGG CCAGGAATAC CGCATGTATA ACACATACGA TGTCCACTTT
    TATGCTTCCT TCGCCCTCAT CATGCTCTGG CCCAAACTTG AGCTCAGCCT TCAGTATGAC
    ATGGCTCTGG CCACTCTCAG GGAGGACCTG ACACAGCGGC GGTTCCTGAT GAGTGGGGTA
    ACAGCGCCTG TGAAGAGGAG GAACGTCATC CCCCATGATG TTGGGGACCC AGATGACGAG
    CCATGGCTCC GGGTCAATGC GTACGAGATC CACGATACTG GAGACTGGAA GGACCTGAAC
    CTGAAGTTTG TGCTGCAGGT CTATCGGGAC TATTACCTGA CGGGTGACCA ATGTTTCTTG
    AGGGACATGT GGCCTGTGTG TCTGGCCGTG ATGGAGTCTG AAATGAAGTT TGACAAGGAC
    CACGATGGAC TCATCGAGAA TGGAGGCTAT GCAGACCAAA CCTATGATGG ATGGGTCACC
    ACAGGCCCCA GTGCTTACTG CGGAGGACTG TGGCTGGCGG CCGTGGCTGT GATGGTCCAG
    ATGGCTGCTC TGTGTGGGGC ACAGGAGGCC CAGAATAAGT TTTCTTCCAT CCTTAGCCGG
    GGCCAGGAAG CCTATGATCG GCTGCTGTGG AATGGCCGCT ACTACAACTA CGACTGCAGC
    TCTCAGCCTC AGTCCTGTAG CATCATGTCT GACCAGTGTG CTGGCCAGTG GTTCCTAAGG
    GCCAGTGGCC TAGGAGAAGG AGACAGTGAG GTCTTTCCTA CCCAACAGGT GGTCCGTGCT
    CTCCAAACGA TCTTTGAGTT CAATGTCCAG GCCTTTGCAG GAGGGGCCAT GGGGGCTGTG
    AATGGCATGC AGCCCTGTGG TGTTCCCGAC ACATCCAGCG TCCAGTCTGA TGAAGTCTGG
    GTGGGCGTGG TCTACGGGCT GGCAGCCACC ATGATCCAAG AGGGCCTGAC TCGGGAGGGC
    TTCCGGACAG CTGAAGGCTG CTATCGTACT GTGTGGGAGC GCCTGGGCCT GGCCTTCCAG
    ACTCCTGAGG CGTACTGCCA GCGGCGGGTG TTCCGCTCAC TGGCCTACAT GCGGCCACTG
    AGCATCTGGG CCATGCAGCT GGCCCTGCAG CAGCAGCAGC AACATAAAAA GGCCTCTGGG
    CCAATATCCA AACGGGGCAC AGGACTATGC ACCAGGCCCA AACGTGGACC AAAGGAAGCC
    ATGGCAAACC CAAGCCCAGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.