MCF2L2 cDNA ORF clone, Bos taurus(cattle)

The following MCF2L2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2L2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219190 XM_024995375.1
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa219187 XM_005201629.4
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa219188 XM_005201630.4
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa219189 XM_005201631.4
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa02585 XM_005201629.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa02585 XM_005192225.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa02586 XM_005192226.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa02586 XM_005201630.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa02587 XM_005201631.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa02587 XM_005192227.3
Latest version!
Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219190
    Clone ID Related Accession (Same CDS sequence) XM_024995375.1
    Accession Version XM_024995375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAAGTGC CGATTGTCAT GTTAAACTCC GTTTCTGACC TTCATGACTA CATTGACAAA 
    AGCCAATTGA CAGCAGACTT AGGGGGAACT TTGGAATATC GCCACAGTCA GTGGATAAAT
    CATCGAACCG CCATTGAAAA CTTTGCTCTA GCATTGAAGA CCACTGCCCA GATGCTTCAG
    ACTTTTGGGG CCTGCCTGGC TACAACAGAG CTGCCCAGAA GCATCCCTTC CACTGAAGAC
    CTTCTCATGT CCCACATGAG GCAGTGGAAC AAGTTGCAGG ATGAGCTGAA ATTACTTGGA
    AAGCAAGGGG CCACATTGCT GTCATGCATG CAAGAACCGG CAACCAAAAG TCCCACCAGC
    AAACTCAGTC TCCATGAAGT TGAGAATGTA GCTACCATGG AAAGGTTATT AGTTCAGTTG
    GATGAAACAC AAAAAGCCTT TATTCAGTTC TGGTCTGAGC ATCACCTGAA GCTTAATCAA
    TACCTACAAC TACAGCACTT TGAACACAGT TTTTGTGAGG TTAAGCTAGC CCTGGATAAC
    TTGTTGGCGG AGCAAGCAGA GTTTTCTGAC ATCGGAGACA GTGTGATGCA TGTGGAACAA
    CTTCTTAAGG AACACAAAAA ACTGGAAGGA GAAGGCCAGG AGTCCTTGGA AAAGGCCCAG
    CTGCTTGCAG TAATTGGGGA CCAGCTTATC CAAAGCCACC ATTATGCAGT GGACTCCATC
    AGACCCAGGT GTGCGGAGCT CAGGCACCTT TGTGATGACT TCATCAATGA AAACAAAAAG
    AAACATGACA TTTTAGAAAA GTCCTTGGAG TTGCACAGAA GGTTGGACAA GGTCAGCCAG
    TGGTGTGAAG CAGGAGTCTA CCTCTTGGCT TCCCAAGCTG TAGACAAGTA CCAGTCCCGA
    GAGGGCATTG ACATTGCCTT GAATGACATT GAGACATTCC TGGGCACAGC AAAGGAGTAC
    CAGTTGCCCA GCTCCAAGGA CTTTTACAAC CAGTATGAGT TGATGCTCAC TCTTGATATA
    AAGACTAAAG CCCAGAAAGT CCTGCAGAAG CTGAGCGATG TGCAGGAAAT ATTTCACAAG
    AGACAAATGA GTCTGATGAA ACTGGCAGCC AGACAGACTC GCCCAGTGCA ACCCGTGGCC
    CCCCATCCTG AGTCCTCCCC AAAATGGGCA TCACCAAAAA TCAGCCAGCC CTCCACTTTG
    GTTCCTCATC AGAAAACATC TGAGGAACCT TATACAGAGA CAGATTTAAA CTCAGAGGAA
    AAGGAAGATG ATGAGACTAA ATCTGAAGTC AAGAATGAAG AAATACTTGA AAGCAGTCAT
    GATGCAGACA ACCCTGAGCC AGAGCAGCCT GATGGTTCCA AAAATCTTTC TCACAGCAGG
    CATATTATAC GTGACTTGGT TGAGACTGAA GTGACTTACA TAAAAGAGAT TAAGAACATA
    ATTGATGGAT ATATCATTCC AATGGATTTT ATCTGGCTGA AGCATCTAAT TCCAGATGTT
    CTGCGGAATA ACAAGGAGTT TCTCTTTGGG AACATTAGAG AACTTTACGA ATTTCACAGC
    AGGACTTTTC TAAAAGAATT GGAAAAATGC ACCGAAAACC CTGAACTTCT GGCACGTTGC
    TTTCTCAAGA GAAAAGAAGA TCTTCAAATA TATTTTAAAT ACCATAAGAA CCTGCCCCGA
    GCCAGGGCAA TCTGGCAAGA ATGTCAAGAC TGCACCTACT TTGGGGTATG CCAGCGTCAA
    CTGGGTCACA GTCTCCCTCT TTTTAAGTGT CTGAGAGAGC CAAGTCAGAG ACTTATAAAG
    TACCAGATGC TGTTGAAGGG GCTGCTGGAT TTTGAGTCTC CTGAGGATTT GGAGATGGAC
    CCAAGTGATC TAGAAGGAGG TTCGGCTAAG AATGGGCCAA AGAGGACCAA GGATTCAACC
    TTCTTAGCTG ACCTACAACA GGCTTTGGCC ATGATGGAGG ATTTGATCAA GTCCTGTGAG
    TTGGCCATGG ACCTGGCAGC AGTCACTGGA TGTCCGGATG ATATTGGAAA ATTCGGCAAG
    TTGTTGATGC ATGGTTCTTT CAACGTTTGG ACAGTTCACA AAGATCGTTA TAAGATGAAG
    GATTTAATTC GATTCAAGCC CAGCCAGAGG CAAATCTATC TATTTGAACG GGAAATAATG
    TTCTGTAAGA TACAGTTGGA GCCCAGTGAC CAGGGCCTGT CCCCTCACTA CAGCTTTAAG
    AAGTCTATGA AGTTGATGAC ACTTTCAATT CGCCAACTTG GAAGGGGGAG CAACAGAAAG
    TTTGAAATTG CCAACCAAAA TGGACTTGAA AAATACATCC TACAGGCAGC TTCAAAAGAA
    ATCAGAGATT GTTGGTTTTC AGAAATAAGT AAATTATTGA TGAAACAACA AAACAATATT
    AAAGATGTTT ATGAAGATGA AGACAATGGC AGACAAATAT CCCTAAAACG TTGCTCAAGC
    AGGGAGTTTA TCTCCATGGA AACCTCTGTC AATTGTGAAG GTGCAGAAGA TTTGGAAAAG
    GAGAACAGTG TCCTGAGTCT CTCGGGACTT TTCCGGTTGG ACGACAGTTA CGAGACCTCC
    ACGTCTGGTC AGGAGAAGGG AGAAAGCATT CGGGGAGAGA AAAAGGGCTG TCAAAAGAAG
    GACACGGCCA CGAACAAAAC TGAGGAGAGC CTTGAGACGT CTACTGGCTT GCTCGCCCCT
    GCGGGGAAGG CCAGTTTCCC CCGAGCAGAG GCAGTGCGGG CAGAGGCAGT GCGGGCGCAA
    GAAGGAAGCC TGAGGCCGCC CTCTGCAGAG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851143.1
    CDS203..2998
    Translation

    Target ORF information:

    RefSeq Version XM_024995375.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAAGTGC CGATTGTCAT GTTAAACTCC GTTTCTGACC TTCATGACTA CATTGACAAA 
    AGCCAATTGA CAGCAGACTT AGGGGGAACT TTGGAATATC GCCACAGTCA GTGGATAAAT
    CATCGAACCG CCATTGAAAA CTTTGCTCTA GCATTGAAGA CCACTGCCCA GATGCTTCAG
    ACTTTTGGGG CCTGCCTGGC TACAACAGAG CTGCCCAGAA GCATCCCTTC CACTGAAGAC
    CTTCTCATGT CCCACATGAG GCAGTGGAAC AAGTTGCAGG ATGAGCTGAA ATTACTTGGA
    AAGCAAGGGG CCACATTGCT GTCATGCATG CAAGAACCGG CAACCAAAAG TCCCACCAGC
    AAACTCAGTC TCCATGAAGT TGAGAATGTA GCTACCATGG AAAGGTTATT AGTTCAGTTG
    GATGAAACAC AAAAAGCCTT TATTCAGTTC TGGTCTGAGC ATCACCTGAA GCTTAATCAA
    TACCTACAAC TACAGCACTT TGAACACAGT TTTTGTGAGG TTAAGCTAGC CCTGGATAAC
    TTGTTGGCGG AGCAAGCAGA GTTTTCTGAC ATCGGAGACA GTGTGATGCA TGTGGAACAA
    CTTCTTAAGG AACACAAAAA ACTGGAAGGA GAAGGCCAGG AGTCCTTGGA AAAGGCCCAG
    CTGCTTGCAG TAATTGGGGA CCAGCTTATC CAAAGCCACC ATTATGCAGT GGACTCCATC
    AGACCCAGGT GTGCGGAGCT CAGGCACCTT TGTGATGACT TCATCAATGA AAACAAAAAG
    AAACATGACA TTTTAGAAAA GTCCTTGGAG TTGCACAGAA GGTTGGACAA GGTCAGCCAG
    TGGTGTGAAG CAGGAGTCTA CCTCTTGGCT TCCCAAGCTG TAGACAAGTA CCAGTCCCGA
    GAGGGCATTG ACATTGCCTT GAATGACATT GAGACATTCC TGGGCACAGC AAAGGAGTAC
    CAGTTGCCCA GCTCCAAGGA CTTTTACAAC CAGTATGAGT TGATGCTCAC TCTTGATATA
    AAGACTAAAG CCCAGAAAGT CCTGCAGAAG CTGAGCGATG TGCAGGAAAT ATTTCACAAG
    AGACAAATGA GTCTGATGAA ACTGGCAGCC AGACAGACTC GCCCAGTGCA ACCCGTGGCC
    CCCCATCCTG AGTCCTCCCC AAAATGGGCA TCACCAAAAA TCAGCCAGCC CTCCACTTTG
    GTTCCTCATC AGAAAACATC TGAGGAACCT TATACAGAGA CAGATTTAAA CTCAGAGGAA
    AAGGAAGATG ATGAGACTAA ATCTGAAGTC AAGAATGAAG AAATACTTGA AAGCAGTCAT
    GATGCAGACA ACCCTGAGCC AGAGCAGCCT GATGGTTCCA AAAATCTTTC TCACAGCAGG
    CATATTATAC GTGACTTGGT TGAGACTGAA GTGACTTACA TAAAAGAGAT TAAGAACATA
    ATTGATGGAT ATATCATTCC AATGGATTTT ATCTGGCTGA AGCATCTAAT TCCAGATGTT
    CTGCGGAATA ACAAGGAGTT TCTCTTTGGG AACATTAGAG AACTTTACGA ATTTCACAGC
    AGGACTTTTC TAAAAGAATT GGAAAAATGC ACCGAAAACC CTGAACTTCT GGCACGTTGC
    TTTCTCAAGA GAAAAGAAGA TCTTCAAATA TATTTTAAAT ACCATAAGAA CCTGCCCCGA
    GCCAGGGCAA TCTGGCAAGA ATGTCAAGAC TGCACCTACT TTGGGGTATG CCAGCGTCAA
    CTGGGTCACA GTCTCCCTCT TTTTAAGTGT CTGAGAGAGC CAAGTCAGAG ACTTATAAAG
    TACCAGATGC TGTTGAAGGG GCTGCTGGAT TTTGAGTCTC CTGAGGATTT GGAGATGGAC
    CCAAGTGATC TAGAAGGAGG TTCGGCTAAG AATGGGCCAA AGAGGACCAA GGATTCAACC
    TTCTTAGCTG ACCTACAACA GGCTTTGGCC ATGATGGAGG ATTTGATCAA GTCCTGTGAG
    TTGGCCATGG ACCTGGCAGC AGTCACTGGA TGTCCGGATG ATATTGGAAA ATTCGGCAAG
    TTGTTGATGC ATGGTTCTTT CAACGTTTGG ACAGTTCACA AAGATCGTTA TAAGATGAAG
    GATTTAATTC GATTCAAGCC CAGCCAGAGG CAAATCTATC TATTTGAACG GGAAATAATG
    TTCTGTAAGA TACAGTTGGA GCCCAGTGAC CAGGGCCTGT CCCCTCACTA CAGCTTTAAG
    AAGTCTATGA AGTTGATGAC ACTTTCAATT CGCCAACTTG GAAGGGGGAG CAACAGAAAG
    TTTGAAATTG CCAACCAAAA TGGACTTGAA AAATACATCC TACAGGCAGC TTCAAAAGAA
    ATCAGAGATT GTTGGTTTTC AGAAATAAGT AAATTATTGA TGAAACAACA AAACAATATT
    AAAGATGTTT ATGAAGATGA AGACAATGGC AGACAAATAT CCCTAAAACG TTGCTCAAGC
    AGGGAGTTTA TCTCCATGGA AACCTCTGTC AATTGTGAAG GTGCAGAAGA TTTGGAAAAG
    GAGAACAGTG TCCTGAGTCT CTCGGGACTT TTCCGGTTGG ACGACAGTTA CGAGACCTCC
    ACGTCTGGTC AGGAGAAGGG AGAAAGCATT CGGGGAGAGA AAAAGGGCTG TCAAAAGAAG
    GACACGGCCA CGAACAAAAC TGAGGAGAGC CTTGAGACGT CTACTGGCTT GCTCGCCCCT
    GCGGGGAAGG CCAGTTTCCC CCGAGCAGAG GCAGTGCGGG CAGAGGCAGT GCGGGCGCAA
    GAAGGAAGCC TGAGGCCGCC CTCTGCAGAG AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219187
    Clone ID Related Accession (Same CDS sequence) XM_005201629.4
    Accession Version XM_005201629.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201629.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCAGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACGTTGCTC AAGCAGGGAG
    TTTATCTCCA TGGAAACCTC TGTCAATTGT GAAGGTGCAG AAGATTTGGA AAAGGAGAAC
    AGTGTCCTGA GTCTCTCGGG ACTTTTCCGG TTGGACGACA GTTACGAGAC CTCCACGTCT
    GGTCAGGAGA AGGGAGAAAG CATTCGGGGA GAGAAAAAGG GCTGTCAAAA GAAGGACACG
    GCCACGAACA AAACTGAGGA GAGCCTTGAG ACGTCTACTG GCTTGCTCGC CCCTGCGGGG
    AAGGCCAGTT TCCCCCGAGC AGAGGCAGTG CGGGCAGAGG CAGTGCGGGC GCAAGAAGGA
    AGCCTGAGGC CGCCCTCTGC AGAGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201686.2
    CDS720..3989
    Translation

    Target ORF information:

    RefSeq Version XM_005201629.4
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201629.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCAGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACGTTGCTC AAGCAGGGAG
    TTTATCTCCA TGGAAACCTC TGTCAATTGT GAAGGTGCAG AAGATTTGGA AAAGGAGAAC
    AGTGTCCTGA GTCTCTCGGG ACTTTTCCGG TTGGACGACA GTTACGAGAC CTCCACGTCT
    GGTCAGGAGA AGGGAGAAAG CATTCGGGGA GAGAAAAAGG GCTGTCAAAA GAAGGACACG
    GCCACGAACA AAACTGAGGA GAGCCTTGAG ACGTCTACTG GCTTGCTCGC CCCTGCGGGG
    AAGGCCAGTT TCCCCCGAGC AGAGGCAGTG CGGGCAGAGG CAGTGCGGGC GCAAGAAGGA
    AGCCTGAGGC CGCCCTCTGC AGAGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219188
    Clone ID Related Accession (Same CDS sequence) XM_005201630.4
    Accession Version XM_005201630.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201630.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCAGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GTGAGTGGAA CGCGGAAAGA AAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201687.2
    CDS720..3776
    Translation

    Target ORF information:

    RefSeq Version XM_005201630.4
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201630.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCAGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GTGAGTGGAA CGCGGAAAGA AAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219189
    Clone ID Related Accession (Same CDS sequence) XM_005201631.4
    Accession Version XM_005201631.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005201631.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCAGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GGCCACGAAC AAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201688.2
    CDS720..3767
    Translation

    Target ORF information:

    RefSeq Version XM_005201631.4
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201631.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCAGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GGCCACGAAC AAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02585
    Clone ID Related Accession (Same CDS sequence) XM_005192225.3 , XM_005201629.3
    Accession Version XM_005192225.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192225.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACGTTGCTC AAGCAGGGAG
    TTTATCTCCA TGGAAACCTC TGTCAATTGT GAAGGTGCAG AAGATTTGGA AAAGGAGAAC
    AGTGTCCTGA GTCTCTCGGG ACTTTTCCGG TTGGACGACA GTTACGAGAC CTCCACGTCT
    GGTCAGGAGA AGGGAGAAAG CATTCGGGGA GAGAAAAAGG GCTGTCAAAA GAAGGACACG
    GCCACGAACA AAACTGAGGA GAGCCTTGAG ACGTCTACTG GCTTGCTCGC CCCTGCGGGG
    AAGGCCAGTT TCCCCCGAGC AGAGGCAGTG CGGGCAGAGG CAGTGCGGGC GCAAGAAGGA
    AGCCTGAGGC CGCCCTCTGC AGAGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005192282.1
    CDS564..3833
    Translation

    Target ORF information:

    RefSeq Version XM_005192225.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005192225.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACGTTGCTC AAGCAGGGAG
    TTTATCTCCA TGGAAACCTC TGTCAATTGT GAAGGTGCAG AAGATTTGGA AAAGGAGAAC
    AGTGTCCTGA GTCTCTCGGG ACTTTTCCGG TTGGACGACA GTTACGAGAC CTCCACGTCT
    GGTCAGGAGA AGGGAGAAAG CATTCGGGGA GAGAAAAAGG GCTGTCAAAA GAAGGACACG
    GCCACGAACA AAACTGAGGA GAGCCTTGAG ACGTCTACTG GCTTGCTCGC CCCTGCGGGG
    AAGGCCAGTT TCCCCCGAGC AGAGGCAGTG CGGGCAGAGG CAGTGCGGGC GCAAGAAGGA
    AGCCTGAGGC CGCCCTCTGC AGAGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02585
    Clone ID Related Accession (Same CDS sequence) XM_005192225.3 , XM_005201629.3
    Accession Version XM_005201629.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3270bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201629.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACGTTGCTC AAGCAGGGAG
    TTTATCTCCA TGGAAACCTC TGTCAATTGT GAAGGTGCAG AAGATTTGGA AAAGGAGAAC
    AGTGTCCTGA GTCTCTCGGG ACTTTTCCGG TTGGACGACA GTTACGAGAC CTCCACGTCT
    GGTCAGGAGA AGGGAGAAAG CATTCGGGGA GAGAAAAAGG GCTGTCAAAA GAAGGACACG
    GCCACGAACA AAACTGAGGA GAGCCTTGAG ACGTCTACTG GCTTGCTCGC CCCTGCGGGG
    AAGGCCAGTT TCCCCCGAGC AGAGGCAGTG CGGGCAGAGG CAGTGCGGGC GCAAGAAGGA
    AGCCTGAGGC CGCCCTCTGC AGAGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201686.1
    CDS564..3833
    Translation

    Target ORF information:

    RefSeq Version XM_005201629.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201629.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACGTTGCTC AAGCAGGGAG
    TTTATCTCCA TGGAAACCTC TGTCAATTGT GAAGGTGCAG AAGATTTGGA AAAGGAGAAC
    AGTGTCCTGA GTCTCTCGGG ACTTTTCCGG TTGGACGACA GTTACGAGAC CTCCACGTCT
    GGTCAGGAGA AGGGAGAAAG CATTCGGGGA GAGAAAAAGG GCTGTCAAAA GAAGGACACG
    GCCACGAACA AAACTGAGGA GAGCCTTGAG ACGTCTACTG GCTTGCTCGC CCCTGCGGGG
    AAGGCCAGTT TCCCCCGAGC AGAGGCAGTG CGGGCAGAGG CAGTGCGGGC GCAAGAAGGA
    AGCCTGAGGC CGCCCTCTGC AGAGAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02586
    Clone ID Related Accession (Same CDS sequence) XM_005192226.3 , XM_005201630.3
    Accession Version XM_005192226.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GTGAGTGGAA CGCGGAAAGA AAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005192283.1
    CDS564..3620
    Translation

    Target ORF information:

    RefSeq Version XM_005192226.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005192226.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GTGAGTGGAA CGCGGAAAGA AAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02586
    Clone ID Related Accession (Same CDS sequence) XM_005192226.3 , XM_005201630.3
    Accession Version XM_005201630.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3057bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GTGAGTGGAA CGCGGAAAGA AAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201687.1
    CDS564..3620
    Translation

    Target ORF information:

    RefSeq Version XM_005201630.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201630.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GTGAGTGGAA CGCGGAAAGA AAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02587
    Clone ID Related Accession (Same CDS sequence) XM_005192227.3 , XM_005201631.3
    Accession Version XM_005192227.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644529.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GGCCACGAAC AAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005192284.1
    CDS564..3611
    Translation

    Target ORF information:

    RefSeq Version XM_005192227.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005192227.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GGCCACGAAC AAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa02587
    Clone ID Related Accession (Same CDS sequence) XM_005192227.3 , XM_005201631.3
    Accession Version XM_005201631.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103813.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005201631.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GGCCACGAAC AAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005201688.1
    CDS564..3611
    Translation

    Target ORF information:

    RefSeq Version XM_005201631.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005201631.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGCTGTTTT GGTTAAAAGA AGAAATGGCC CCTCGGGAGT TCACACGGCG ACTGGCCACA 
    GTGATCACTC ATGTCGATGA AATTATGCAG CAGGAAATCA GGCCCTTGGT GGCAGTGGAT
    ATAATTGAAC AACTTCACAG ACAATTTGCC ATTCTTTCAG GAGGCCGAGG GAAAGATGGT
    GCCCCCATCA TCACTTTTCC AGAGTTTGTG GGGTTCAAGC ACATCCCAGA TGAAGACTTC
    CTGAATGTCA TGACCTACCT AACTAGTGTC CCCAGCATGG AGGCTGCCAG CATCGGGTTC
    GTCATTGTTA TCGACAGACG AAGGGACAAG TGGAGCTCCA TAAAAGCATC CTTGACACGA
    ATAGCTGTAG CATTTCCAGG AAACTTGCAG CTCATACTCA TTCTTCGTCC ATCTCGATTT
    ATCCAGAGAA CATTCACTGA CATTGGCATT AAGTACTATA GAGATGAATT TAAAATGAAA
    GTGCCGATTG TCATGTTAAA CTCCGTTTCT GACCTTCATG ACTACATTGA CAAAAGCCAA
    TTGACAGCAG ACTTAGGGGG AACTTTGGAA TATCGCCACA GTCAGTGGAT AAATCATCGA
    ACCGCCATTG AAAACTTTGC TCTAGCATTG AAGACCACTG CCCAGATGCT TCAGACTTTT
    GGGGCCTGCC TGGCTACAAC AGAGCTGCCC AGAAGCATCC CTTCCACTGA AGACCTTCTC
    ATGTCCCACA TGAGGCAGTG GAACAAGTTG CAGGATGAGC TGAAATTACT TGGAAAGCAA
    GGGGCCACAT TGCTGTCATG CATGCAAGAA CCGGCAACCA AAAGTCCCAC CAGCAAACTC
    AGTCTCCATG AAGTTGAGAA TGTAGCTACC ATGGAAAGGT TATTAGTTCA GTTGGATGAA
    ACACAAAAAG CCTTTATTCA GTTCTGGTCT GAGCATCACC TGAAGCTTAA TCAATACCTA
    CAACTACAGC ACTTTGAACA CAGTTTTTGT GAGGTTAAGC TAGCCCTGGA TAACTTGTTG
    GCGGAGCAAG CAGAGTTTTC TGACATCGGA GACAGTGTGA TGCATGTGGA ACAACTTCTT
    AAGGAACACA AAAAACTGGA AGGAGAAGGC CAGGAGTCCT TGGAAAAGGC CCAGCTGCTT
    GCAGTAATTG GGGACCAGCT TATCCAAAGC CACCATTATG CAGTGGACTC CATCAGACCC
    AGGTGTGCGG AGCTCAGGCA CCTTTGTGAT GACTTCATCA ATGAAAACAA AAAGAAACAT
    GACATTTTAG AAAAGTCCTT GGAGTTGCAC AGAAGGTTGG ACAAGGTCAG CCAGTGGTGT
    GAAGCAGGAG TCTACCTCTT GGCTTCCCAA GCTGTAGACA AGTACCAGTC CCGAGAGGGC
    ATTGACATTG CCTTGAATGA CATTGAGACA TTCCTGGGCA CAGCAAAGGA GTACCAGTTG
    CCCAGCTCCA AGGACTTTTA CAACCAGTAT GAGTTGATGC TCACTCTTGA TATAAAGACT
    AAAGCCCAGA AAGTCCTGCA GAAGCTGAGC GATGTGCAGG AAATATTTCA CAAGAGACAA
    ATGAGTCTGA TGAAACTGGC AGCCAGACAG ACTCGCCCAG TGCAACCCGT GGCCCCCCAT
    CCTGAGTCCT CCCCAAAATG GGCATCACCA AAAATCAGCC AGCCCTCCAC TTTGGTTCCT
    CATCAGAAAA CATCTGAGGA ACCTTATACA GAGACAGATT TAAACTCAGA GGAAAAGGAA
    GATGATGAGA CTAAATCTGA AGTCAAGAAT GAAGAAATAC TTGAAAGCAG TCATGATGCA
    GACAACCCTG AGCCAGAGCA GCCTGATGGT TCCAAAAATC TTTCTCACAG CAGGCATATT
    ATACGTGACT TGGTTGAGAC TGAAGTGACT TACATAAAAG AGATTAAGAA CATAATTGAT
    GGATATATCA TTCCAATGGA TTTTATCTGG CTGAAGCATC TAATTCCAGA TGTTCTGCGG
    AATAACAAGG AGTTTCTCTT TGGGAACATT AGAGAACTTT ACGAATTTCA CAGCAGGACT
    TTTCTAAAAG AATTGGAAAA ATGCACCGAA AACCCTGAAC TTCTGGCACG TTGCTTTCTC
    AAGAGAAAAG AAGATCTTCA AATATATTTT AAATACCATA AGAACCTGCC CCGAGCCAGG
    GCAATCTGGC AAGAATGTCA AGACTGCACC TACTTTGGGG TATGCCAGCG TCAACTGGGT
    CACAGTCTCC CTCTTTTTAA GTGTCTGAGA GAGCCAAGTC AGAGACTTAT AAAGTACCAG
    ATGCTGTTGA AGGGGCTGCT GGATTTTGAG TCTCCTGAGG ATTTGGAGAT GGACCCAAGT
    GATCTAGAAG GAGGTTCGGC TAAGAATGGG CCAAAGAGGA CCAAGGATTC AACCTTCTTA
    GCTGACCTAC AACAGGCTTT GGCCATGATG GAGGATTTGA TCAAGTCCTG TGAGTTGGCC
    ATGGACCTGG CAGCAGTCAC TGGATGTCCG GATGATATTG GAAAATTCGG CAAGTTGTTG
    ATGCATGGTT CTTTCAACGT TTGGACAGTT CACAAAGATC GTTATAAGAT GAAGGATTTA
    ATTCGATTCA AGCCCAGCCA GAGGCAAATC TATCTATTTG AACGGGAAAT AATGTTCTGT
    AAGATACAGT TGGAGCCCAG TGACCAGGGC CTGTCCCCTC ACTACAGCTT TAAGAAGTCT
    ATGAAGTTGA TGACACTTTC AATTCGCCAA CTTGGAAGGG GGAGCAACAG AAAGTTTGAA
    ATTGCCAACC AAAATGGACT TGAAAAATAC ATCCTACAGG CAGCTTCAAA AGAAATCAGA
    GATTGTTGGT TTTCAGAAAT AAGTAAATTA TTGATGAAAC AACAAAACAA TATTAAAGAT
    GTTTATGAAG ATGAAGACAA TGGCAGACAA ATATCCCTAA AACCTCTCGG GACTTTTCCG
    GTTGGACGAC AGTTACGAGA CCTCCACGTC TGGTCAGGAG AAGGGAGAAA GCATTCGGGG
    AGAGAAAAAG GGCTGTCAAA AGAAGGACAC GGCCACGAAC AAAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.