LOC100652255 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100652255
Entrez Gene ID	100652255
Full Name	eukaryotic translation initiation factor 3 subunit B
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003400774.3	XP_003400822.1	eukaryotic translation initiation factor 3 subunit B

Pathways from BioSystems
Gene Ontology

The following LOC100652255 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100652255 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo62638	XM_003400774.3 Latest version!	Bombus terrestris eukaryotic translation initiation factor 3 subunit B (LOC100652255), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100652255 ( XM_003400774.3 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_003400822.1 Bombus terrestris eukaryotic translation initiation factor 3 subunit B (LOC100652255), mRNA.

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CloneID	OBo62638
Clone ID Related Accession (Same CDS sequence)	XM_003400774.3
Accession Version	XM_003400774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	eukaryotic translation initiation factor 3 subunit B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015775.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_003400774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGA AAACGTTAGC AGATAAAACA CTTCAAAACG AAGATAATGT TAAAAATAAT 
GATGATATAT TTAACGACGA TGAAGAGCCA AATTTCAGCG ATCCTGAAGG ATTTGTGGAC 
GATATTACCG ACGAAGAACT CCTTGGTGAT ATCTTGAAGC AAAAACCATC AGAGACAGAT 
GGAGTGGAAT CCGTAATCGT CGTTGATGGA GTTCCCCAGG TTGAACCAGA AAGATTAGAA 
AAACTTCAAT CCGTCATTAG CAAAGTCTTA GGAAAATTTG GCACAATAGT GAATCAATAT 
TATCCCAAAA ATGAAAATGG ATATACAAAA GGGTACATAT TTCTAGAATA TAATAATCCT 
CTTAATGCTT TAGCAGCTGC CCAATCTGTA AATAATTACA AAATTGACAA GCAACATACG 
TTCAAAGTAA ATTTGTTTAC CGATTTTAAG AAATTTGAAG AAATTGCAGA TGAATGGGAA 
CCTCCAAAAC CACAAGAATT TAAAGCTGCA GCAGATTTAC ATTATTATCT TTTAGAACCA 
GATGCTTATG ACCAATTTTG TGTACTTTGT GGTAATGGTC CTTCTGTTAC TGTTCAAGTC 
TGGCAGAATT CTGCTCCTGA ACCAACATTG CTTGAAGAAC GTTCTCGTTG GACTGAGACG 
TATGTTAAAT GGTCTCCATT GGGCACATAT TTAGCAACAT TTCACAAATT GGGTGTTGCA 
TTATGGGGTG GTCCTCAATT TTTACAACAA GCCAGATTCA GTCAGAAGGG TGTTGAATGT 
ATTGACTTCT CTCCTTGCGA ACGTTACTTA GTAACATACA CTCCGCGAAC TGATCTTGGT 
TCAGATCAGA AACGACTAGT AATTTGGGAC ATTCTTACTG GACAAGAAAA ACGTGCGTTT 
TTCGCAGATG GTTCTTCAGT ATGGCCTATT TTCCGCTGGT CGCATGATGA TAAATATCTT 
GCTCGTATGG GGGAAGATGT TTTGAGTGTA TATGAAACAC CATCATTTGG ATTATTGGAT 
AAGAAAAGTA TAAAAATTCC CGGTATCAGA GATTTTAGTT GGTCTCCCAC AGATAACGTT 
CTTGCTTACT GGGTACCAGA AGATAAAGAT GTTCCAGCTA GAGTCACTCT TCTCGAAATT 
CCAAACCGAA ATGAAATTCG TAACAAGAAC TTGTTTAATG TAGCTGATTG TAAAATTTTT 
TGGCAAAAGT CAGGAGATTA TCTCTGTGTA AAAGTCGACC GTTTTGCCAA ACGCAAAGAG 
AAAACAGAAC AGAAATATAC GGGTATGTCT TATAACTTTG AAATCTTTCA TATGAGGGAA 
AAGCAAATTC CAGTCGATAG TGTAGAAATA AAAGAACCAA TCCATGCTTT TGCTTGGGAA 
CCTGTTGGAA GCAAATTTGC TATTATTCAC GGAGAGATTC CAAGCGTAAA CGTTAGCTTT 
TATGAAGTAC GATACGGTCA TCAACCAGCC CTTCTTAAAA AATTAGAGAA GAAGGCTTGT 
AATCATCTAT TTTGGTCGCC ATCTGGACAA TTTATTGTTC TTGCTGGATT GACAACCATG 
GCAGGGGCTT TGGAATTCAT TGATACTAAC GATTTTACTA TCATGAATTC AACTGACCAT 
TATCAAACTT CAGATGTCGA ATGGGATCCT ACTGGACGGT ATGTAGTAAC AGCAGTTTCG 
AGTTGGAAAA CTAGCGTAGA TAATGGATAC TGGATATGGA CATTCCAAGG ACGTATCTTA 
AAAAGAGTTA ATCTTAATCA GTTCAATCAA TTATTATGGA GACCGAGGCC ACCAACGTTA 
TTAACACCAG AACAAATTAA GGAAATTAAG AAAAATTTAA AGAAATATTC TGCACAATTT 
GAAAGCAAAG ATCGTATGCG ACTGACGCGC GCCTCGAAGG AATTAATCGA AAAACGCTGC 
GCAGCGATGA AAGCATTTGA AGAATATCGT TCAAAACGTA TACAAGAATG GAATAACCAA 
AAGAAACGAC GTTTAGAATT ACGTAATGCT ATCGACACCG ACGAATTAGA TTCTGATTCA 
AAGAATGTAG AAGAAGAAGT AATAGAATTT TTCGTCAAAG AAGAAACATT CATCATCGAC 
GAGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003400822.1
CDS	47..2155
Translation	MAKKTLADKTLQNEDNVKNNDDIFNDDEEPNFSDPEGFVDDITDEELLGDILKQKPSETDGVESVIVVDGVPQVEPERLEKLQSVISKVLGKFGTIVNQYYPKNENGYTKGYIFLEYNNPLNALAAAQSVNNYKIDKQHTFKVNLFTDFKKFEEIADEWEPPKPQEFKAAADLHYYLLEPDAYDQFCVLCGNGPSVTVQVWQNSAPEPTLLEERSRWTETYVKWSPLGTYLATFHKLGVALWGGPQFLQQARFSQKGVECIDFSPCERYLVTYTPRTDLGSDQKRLVIWDILTGQEKRAFFADGSSVWPIFRWSHDDKYLARMGEDVLSVYETPSFGLLDKKSIKIPGIRDFSWSPTDNVLAYWVPEDKDVPARVTLLEIPNRNEIRNKNLFNVADCKIFWQKSGDYLCVKVDRFAKRKEKTEQKYTGMSYNFEIFHMREKQIPVDSVEIKEPIHAFAWEPVGSKFAIIHGEIPSVNVSFYEVRYGHQPALLKKLEKKACNHLFWSPSGQFIVLAGLTTMAGALEFIDTNDFTIMNSTDHYQTSDVEWDPTGRYVVTAVSSWKTSVDNGYWIWTFQGRILKRVNLNQFNQLLWRPRPPTLLTPEQIKEIKKNLKKYSAQFESKDRMRLTRASKELIEKRCAAMKAFEEYRSKRIQEWNNQKKRRLELRNAIDTDELDSDSKNVEEEVIEFFVKEETFIIDEK

Target ORF information:

RefSeq Version	XM_003400774.3
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris eukaryotic translation initiation factor 3 subunit B (LOC100652255), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003400774.3

ORF Insert Sequence:

ATGGCGAAGA AAACGTTAGC AGATAAAACA CTTCAAAACG AAGATAATGT TAAAAATAAT 
GATGATATAT TTAACGACGA TGAAGAGCCA AATTTCAGCG ATCCTGAAGG ATTTGTGGAC 
GATATTACCG ACGAAGAACT CCTTGGTGAT ATCTTGAAGC AAAAACCATC AGAGACAGAT 
GGAGTGGAAT CCGTAATCGT CGTTGATGGA GTTCCCCAGG TTGAACCAGA AAGATTAGAA 
AAACTTCAAT CCGTCATTAG CAAAGTCTTA GGAAAATTTG GCACAATAGT GAATCAATAT 
TATCCCAAAA ATGAAAATGG ATATACAAAA GGGTACATAT TTCTAGAATA TAATAATCCT 
CTTAATGCTT TAGCAGCTGC CCAATCTGTA AATAATTACA AAATTGACAA GCAACATACG 
TTCAAAGTAA ATTTGTTTAC CGATTTTAAG AAATTTGAAG AAATTGCAGA TGAATGGGAA 
CCTCCAAAAC CACAAGAATT TAAAGCTGCA GCAGATTTAC ATTATTATCT TTTAGAACCA 
GATGCTTATG ACCAATTTTG TGTACTTTGT GGTAATGGTC CTTCTGTTAC TGTTCAAGTC 
TGGCAGAATT CTGCTCCTGA ACCAACATTG CTTGAAGAAC GTTCTCGTTG GACTGAGACG 
TATGTTAAAT GGTCTCCATT GGGCACATAT TTAGCAACAT TTCACAAATT GGGTGTTGCA 
TTATGGGGTG GTCCTCAATT TTTACAACAA GCCAGATTCA GTCAGAAGGG TGTTGAATGT 
ATTGACTTCT CTCCTTGCGA ACGTTACTTA GTAACATACA CTCCGCGAAC TGATCTTGGT 
TCAGATCAGA AACGACTAGT AATTTGGGAC ATTCTTACTG GACAAGAAAA ACGTGCGTTT 
TTCGCAGATG GTTCTTCAGT ATGGCCTATT TTCCGCTGGT CGCATGATGA TAAATATCTT 
GCTCGTATGG GGGAAGATGT TTTGAGTGTA TATGAAACAC CATCATTTGG ATTATTGGAT 
AAGAAAAGTA TAAAAATTCC CGGTATCAGA GATTTTAGTT GGTCTCCCAC AGATAACGTT 
CTTGCTTACT GGGTACCAGA AGATAAAGAT GTTCCAGCTA GAGTCACTCT TCTCGAAATT 
CCAAACCGAA ATGAAATTCG TAACAAGAAC TTGTTTAATG TAGCTGATTG TAAAATTTTT 
TGGCAAAAGT CAGGAGATTA TCTCTGTGTA AAAGTCGACC GTTTTGCCAA ACGCAAAGAG 
AAAACAGAAC AGAAATATAC GGGTATGTCT TATAACTTTG AAATCTTTCA TATGAGGGAA 
AAGCAAATTC CAGTCGATAG TGTAGAAATA AAAGAACCAA TCCATGCTTT TGCTTGGGAA 
CCTGTTGGAA GCAAATTTGC TATTATTCAC GGAGAGATTC CAAGCGTAAA CGTTAGCTTT 
TATGAAGTAC GATACGGTCA TCAACCAGCC CTTCTTAAAA AATTAGAGAA GAAGGCTTGT 
AATCATCTAT TTTGGTCGCC ATCTGGACAA TTTATTGTTC TTGCTGGATT GACAACCATG 
GCAGGGGCTT TGGAATTCAT TGATACTAAC GATTTTACTA TCATGAATTC AACTGACCAT 
TATCAAACTT CAGATGTCGA ATGGGATCCT ACTGGACGGT ATGTAGTAAC AGCAGTTTCG 
AGTTGGAAAA CTAGCGTAGA TAATGGATAC TGGATATGGA CATTCCAAGG ACGTATCTTA 
AAAAGAGTTA ATCTTAATCA GTTCAATCAA TTATTATGGA GACCGAGGCC ACCAACGTTA 
TTAACACCAG AACAAATTAA GGAAATTAAG AAAAATTTAA AGAAATATTC TGCACAATTT 
GAAAGCAAAG ATCGTATGCG ACTGACGCGC GCCTCGAAGG AATTAATCGA AAAACGCTGC 
GCAGCGATGA AAGCATTTGA AGAATATCGT TCAAAACGTA TACAAGAATG GAATAACCAA 
AAGAAACGAC GTTTAGAATT ACGTAATGCT ATCGACACCG ACGAATTAGA TTCTGATTCA 
AAGAATGTAG AAGAAGAAGT AATAGAATTT TTCGTCAAAG AAGAAACATT CATCATCGAC 
GAGAAATAA