LOC100648332 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100648332
Entrez Gene ID	100648332
Full Name	kinesin-like protein Klp61F
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012313322.2	XP_012168712.1	kinesin-like protein Klp61F isoform X2
XM_012313321.2	XP_012168711.1	kinesin-like protein Klp61F isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100648332 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100648332 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo59854	XM_012313321.2 Latest version!	Bombus terrestris kinesin-like protein Klp61F (LOC100648332), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo59855	XM_012313322.2 Latest version!	Bombus terrestris kinesin-like protein Klp61F (LOC100648332), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100648332 ( XM_012313321.2 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_012168711.1 Bombus terrestris kinesin-like protein Klp61F (LOC100648332), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo59854
Clone ID Related Accession (Same CDS sequence)	XM_012313321.2
Accession Version	XM_012313321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	kinesin-like protein Klp61F isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_012313321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGATA CACGGAGTGG AAGAAAGGAA AAATGTCAGC AGATACAAGT ATTTGTTCGT 
GTTAGACCTA TCAACAATTT TGAGGAAAAT AATAAATCTA CATCCATATT AGAAGTTCCA 
AATAATAGGG AAATAATAGT ACATGAACGT CCAAATGACA AAGTTTCTAA AAAATTTAAG 
TTTAATAATG TATTTGGACC TTTGTCAAAA CAGATCGATG TATATAATGT CGTTGTTAGT 
CCATTATTGG AACAAGTATT GGCTGGATAT AATTGTACAG TATTTGCATA TGGTCAAACT 
GGTACAGGGA AGACATACAC AATGGAAGGA ATTAACAATG ATCGAACATT ATATTGGCAC 
AGTGACTCGT CTGCTGGCAT GATACCACGT TCTTTAAGTC ACTTATTTGA CAAATTGCAA 
CTACTGGAAA CACAGGAATA TACAATTAGA GTTAGTTTTC TTGAACTCTA TAATGAAGAC 
ATATTTGATC TTTTATCATC TAGTAATGAT GCATGTAAAT TGAGGCTATA TGAAGATGCA 
TCAAAGAAAG GTGCAGTAAT CATACATGGA TTAGAAGAAG TAACAATACA CAATGCAAGT 
GAAGTTTATA AAATTATTAA GAAAGGGTCT GACAGAAGGC AAACAGCTGC AACATTGATG 
AATACTCAAT CTAGATGCCG GTCCCATACT ATATTCTCAA TTACTATACA TATGAAAGAA 
AGTACTGGTA ATACAGATGG TGAAGAGATT CTGAAAATGG GGAAATTAAA TTTAGTGGAT 
CTGGCTGGTA GTGAAAATAT TGGAAGATCT GGCTCTGTTG ATAGGAGAGC AAGAGAAGCT 
GGTAATATTA ACCAGTCTTT ATTAACTCTT GGCAGAGTTA TAACAGCTCT TGTTGAAAAA 
GCACCTCATA TACCTTACCG GGAATCTAAA CTTACAAGAT TACTGCAGGA ATCATTAGGT 
GGTCGGACAA AAACGTCAAT TATCGCAACA GTATCCCCTG CTAGTATTAA CCTTGAGGAA 
ACATTATCAA CGCTAGATTA TGCACATCGT GCAAAAAATA TCACAAATCG TCCAGAAATA 
AATCAGAAAC TTTCGAAACG AGAATTTTTG AAACAATATA CAGAAGAAAT TGAAAAGCTG 
CGAAGAGATT TATCAGCTTC ACGTGATAGA AATGGTGTTT ATCTTGCAGA TGATAATTAT 
AGAGAAATGG AAACACTAAT TTCACGACAG ACTAAGGAAA TAGAAGAAAA AATTAATCAT 
ATAAAAGCGC TTGAGAAAAT TATGCAAGAC AAGGAGAAAA TATTTAATGA ACTTGAATTA 
CAAAATATTG CCCAGACAAA AGAATTAGAT GAAGTTAAGA CTAAATTAAA TAATTTCTCT 
GAAGCTCTAC AGTCGTCAAA TTATCGATTA AAATTAACAA CGCAGGAACG AGATGAGCAT 
AAATATTTAG TAGAGAGACA TATGAATACA GAACAATCTT TATTGGATCA AGCTCAGACT 
TTGCTAAATG TTGCAGATAC AGCAATAACA GATTCATATA AATTACACGA TAAAATTGAC 
AGGAAAAGTA AAACAGAACA AAAATTTGAA GACCTTGGTA AGACCGTGAA AAGTAATGTT 
TGTGAATGTT TGCAAAATAT AGAAAAAAAT ATATCTACAT ATGGTGAAAC ATTAAAGAGA 
TTTTTTCCAG CAATGAAAAA TGACATTGAA ACCTCTATCA AATGTAACGG TATTGACACG 
ATAATACATC GAAATGCGAC GAGCAATTCG AATCAATCAG ATACTACTAA TTTAACAAAA 
AATATAAATG AATTTCATTT AGAGTATCAG AATTGGATAC AAAATGAAGT AAGAAATGTG 
GTTGGCACAG TGGAATCTGA ACATGAATTA ATGAGTATCG TTTCTCTGCA ATTGGCTGAA 
AACATCAACA GTATGATAGA AAATGAAATA GCTAAAAACA TGGAAATCAT AAGGAATAAT 
GTATCTGAAA AATTAAAACA AACTTCAACT CTTCTAAAAG AAATGATAGA TGCTTCTTGT 
AATTATGAAT TAGAAATTAA TGATCGTATG AGCCAAAGTG TCAAAAGTAT TATGGAAGCT 
GTTGAAGTAC TTCGTAAAAA ACAGGAATTT AGTGAAAAAC AACAGTTATT TACTAAAATG 
ATGGGAGATC TCTTTTCACA GTCAAATGAA TTACATAATA GTCAAGGAAA GCATTATTCT 
GCTACAATTG ACAAATGTGA TTATGTTAGC AAAATTTGTA ATGAAGCTAA TGTTCAAGCA 
ACAAATAGTT CTAATATAAA TGTTAAAATG AGAAATGATT TAAAAGATTA TATACAAAGG 
GATATAGAGA AAATTGAAAA TAACGTTATT GTTACAACAA GACAGAATGA AGAAATTGCA 
AATGAGATTA CAAAGCGAGG AGAGGATTTA ATAAACGAAT TAAAGTCAAA TATAAATGAA 
AGTTGTAATA TGTCGAAGCA GTATATAAAT GATATGGAAT GCAATATTAA GGAAAAGCAA 
CTGAAAATGG ATGCAGATAA GAATATATCC CTTTCATTAA TCAATGATGC ACATGAAGTA 
ATTTCAGATA TAAGTAAAAA CCAAGCAAGA TTCGTGAAAG ATATGAAGGA AAATACTGGA 
TATGTGTCTC AAGACGTATC TGCCCAATTA GAAAACCAAA TAAGGGAATC AAGTACACGG 
AATGATAATA TTATTGAACA GTTACAAATA ACAGTTGGTA AAATTAACAA ATTTTTCGAT 
GAAGATTTGC AACGTGATGT CGCAACAGGA TTAACTCCTG TAAGGAAAGA CTTTTCCTAT 
CCTCGTCAAT TTAAGGTAAC ATCTCCACAT GAGCAAATTC TTAAAAAGTT TAGAGAAAGC 
AGAAAATTCC TTGAAACATC AGATGATGAT TCTGTACAAG ATGACACAAT CATCAATGGT 
GAAATAATGA AAGGCATTGC AAATTCAACT GCATTATCTG ACATTACACT CGTATCTTCT 
GCAATTGATA ATATAACATT TGATACCTCT ATTTCAAGTA CTCATACTGA AAATATTACA 
CAAATGTTAG ATGTATCTTC AAAATCGATA CAATCCCATA CTCAATCTGA ATCTGCAATA 
TACAGAAAGA ATGAAAACAA GGAGAATAAT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012168711.1
CDS	198..3353
Translation	MNDTRSGRKEKCQQIQVFVRVRPINNFEENNKSTSILEVPNNREIIVHERPNDKVSKKFKFNNVFGPLSKQIDVYNVVVSPLLEQVLAGYNCTVFAYGQTGTGKTYTMEGINNDRTLYWHSDSSAGMIPRSLSHLFDKLQLLETQEYTIRVSFLELYNEDIFDLLSSSNDACKLRLYEDASKKGAVIIHGLEEVTIHNASEVYKIIKKGSDRRQTAATLMNTQSRCRSHTIFSITIHMKESTGNTDGEEILKMGKLNLVDLAGSENIGRSGSVDRRAREAGNINQSLLTLGRVITALVEKAPHIPYRESKLTRLLQESLGGRTKTSIIATVSPASINLEETLSTLDYAHRAKNITNRPEINQKLSKREFLKQYTEEIEKLRRDLSASRDRNGVYLADDNYREMETLISRQTKEIEEKINHIKALEKIMQDKEKIFNELELQNIAQTKELDEVKTKLNNFSEALQSSNYRLKLTTQERDEHKYLVERHMNTEQSLLDQAQTLLNVADTAITDSYKLHDKIDRKSKTEQKFEDLGKTVKSNVCECLQNIEKNISTYGETLKRFFPAMKNDIETSIKCNGIDTIIHRNATSNSNQSDTTNLTKNINEFHLEYQNWIQNEVRNVVGTVESEHELMSIVSLQLAENINSMIENEIAKNMEIIRNNVSEKLKQTSTLLKEMIDASCNYELEINDRMSQSVKSIMEAVEVLRKKQEFSEKQQLFTKMMGDLFSQSNELHNSQGKHYSATIDKCDYVSKICNEANVQATNSSNINVKMRNDLKDYIQRDIEKIENNVIVTTRQNEEIANEITKRGEDLINELKSNINESCNMSKQYINDMECNIKEKQLKMDADKNISLSLINDAHEVISDISKNQARFVKDMKENTGYVSQDVSAQLENQIRESSTRNDNIIEQLQITVGKINKFFDEDLQRDVATGLTPVRKDFSYPRQFKVTSPHEQILKKFRESRKFLETSDDDSVQDDTIINGEIMKGIANSTALSDITLVSSAIDNITFDTSISSTHTENITQMLDVSSKSIQSHTQSESAIYRKNENKENNN

Target ORF information:

RefSeq Version	XM_012313321.2
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris kinesin-like protein Klp61F (LOC100648332), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012313321.2

ORF Insert Sequence:

ATGAACGATA CACGGAGTGG AAGAAAGGAA AAATGTCAGC AGATACAAGT ATTTGTTCGT 
GTTAGACCTA TCAACAATTT TGAGGAAAAT AATAAATCTA CATCCATATT AGAAGTTCCA 
AATAATAGGG AAATAATAGT ACATGAACGT CCAAATGACA AAGTTTCTAA AAAATTTAAG 
TTTAATAATG TATTTGGACC TTTGTCAAAA CAGATCGATG TATATAATGT CGTTGTTAGT 
CCATTATTGG AACAAGTATT GGCTGGATAT AATTGTACAG TATTTGCATA TGGTCAAACT 
GGTACAGGGA AGACATACAC AATGGAAGGA ATTAACAATG ATCGAACATT ATATTGGCAC 
AGTGACTCGT CTGCTGGCAT GATACCACGT TCTTTAAGTC ACTTATTTGA CAAATTGCAA 
CTACTGGAAA CACAGGAATA TACAATTAGA GTTAGTTTTC TTGAACTCTA TAATGAAGAC 
ATATTTGATC TTTTATCATC TAGTAATGAT GCATGTAAAT TGAGGCTATA TGAAGATGCA 
TCAAAGAAAG GTGCAGTAAT CATACATGGA TTAGAAGAAG TAACAATACA CAATGCAAGT 
GAAGTTTATA AAATTATTAA GAAAGGGTCT GACAGAAGGC AAACAGCTGC AACATTGATG 
AATACTCAAT CTAGATGCCG GTCCCATACT ATATTCTCAA TTACTATACA TATGAAAGAA 
AGTACTGGTA ATACAGATGG TGAAGAGATT CTGAAAATGG GGAAATTAAA TTTAGTGGAT 
CTGGCTGGTA GTGAAAATAT TGGAAGATCT GGCTCTGTTG ATAGGAGAGC AAGAGAAGCT 
GGTAATATTA ACCAGTCTTT ATTAACTCTT GGCAGAGTTA TAACAGCTCT TGTTGAAAAA 
GCACCTCATA TACCTTACCG GGAATCTAAA CTTACAAGAT TACTGCAGGA ATCATTAGGT 
GGTCGGACAA AAACGTCAAT TATCGCAACA GTATCCCCTG CTAGTATTAA CCTTGAGGAA 
ACATTATCAA CGCTAGATTA TGCACATCGT GCAAAAAATA TCACAAATCG TCCAGAAATA 
AATCAGAAAC TTTCGAAACG AGAATTTTTG AAACAATATA CAGAAGAAAT TGAAAAGCTG 
CGAAGAGATT TATCAGCTTC ACGTGATAGA AATGGTGTTT ATCTTGCAGA TGATAATTAT 
AGAGAAATGG AAACACTAAT TTCACGACAG ACTAAGGAAA TAGAAGAAAA AATTAATCAT 
ATAAAAGCGC TTGAGAAAAT TATGCAAGAC AAGGAGAAAA TATTTAATGA ACTTGAATTA 
CAAAATATTG CCCAGACAAA AGAATTAGAT GAAGTTAAGA CTAAATTAAA TAATTTCTCT 
GAAGCTCTAC AGTCGTCAAA TTATCGATTA AAATTAACAA CGCAGGAACG AGATGAGCAT 
AAATATTTAG TAGAGAGACA TATGAATACA GAACAATCTT TATTGGATCA AGCTCAGACT 
TTGCTAAATG TTGCAGATAC AGCAATAACA GATTCATATA AATTACACGA TAAAATTGAC 
AGGAAAAGTA AAACAGAACA AAAATTTGAA GACCTTGGTA AGACCGTGAA AAGTAATGTT 
TGTGAATGTT TGCAAAATAT AGAAAAAAAT ATATCTACAT ATGGTGAAAC ATTAAAGAGA 
TTTTTTCCAG CAATGAAAAA TGACATTGAA ACCTCTATCA AATGTAACGG TATTGACACG 
ATAATACATC GAAATGCGAC GAGCAATTCG AATCAATCAG ATACTACTAA TTTAACAAAA 
AATATAAATG AATTTCATTT AGAGTATCAG AATTGGATAC AAAATGAAGT AAGAAATGTG 
GTTGGCACAG TGGAATCTGA ACATGAATTA ATGAGTATCG TTTCTCTGCA ATTGGCTGAA 
AACATCAACA GTATGATAGA AAATGAAATA GCTAAAAACA TGGAAATCAT AAGGAATAAT 
GTATCTGAAA AATTAAAACA AACTTCAACT CTTCTAAAAG AAATGATAGA TGCTTCTTGT 
AATTATGAAT TAGAAATTAA TGATCGTATG AGCCAAAGTG TCAAAAGTAT TATGGAAGCT 
GTTGAAGTAC TTCGTAAAAA ACAGGAATTT AGTGAAAAAC AACAGTTATT TACTAAAATG 
ATGGGAGATC TCTTTTCACA GTCAAATGAA TTACATAATA GTCAAGGAAA GCATTATTCT 
GCTACAATTG ACAAATGTGA TTATGTTAGC AAAATTTGTA ATGAAGCTAA TGTTCAAGCA 
ACAAATAGTT CTAATATAAA TGTTAAAATG AGAAATGATT TAAAAGATTA TATACAAAGG 
GATATAGAGA AAATTGAAAA TAACGTTATT GTTACAACAA GACAGAATGA AGAAATTGCA 
AATGAGATTA CAAAGCGAGG AGAGGATTTA ATAAACGAAT TAAAGTCAAA TATAAATGAA 
AGTTGTAATA TGTCGAAGCA GTATATAAAT GATATGGAAT GCAATATTAA GGAAAAGCAA 
CTGAAAATGG ATGCAGATAA GAATATATCC CTTTCATTAA TCAATGATGC ACATGAAGTA 
ATTTCAGATA TAAGTAAAAA CCAAGCAAGA TTCGTGAAAG ATATGAAGGA AAATACTGGA 
TATGTGTCTC AAGACGTATC TGCCCAATTA GAAAACCAAA TAAGGGAATC AAGTACACGG 
AATGATAATA TTATTGAACA GTTACAAATA ACAGTTGGTA AAATTAACAA ATTTTTCGAT 
GAAGATTTGC AACGTGATGT CGCAACAGGA TTAACTCCTG TAAGGAAAGA CTTTTCCTAT 
CCTCGTCAAT TTAAGGTAAC ATCTCCACAT GAGCAAATTC TTAAAAAGTT TAGAGAAAGC 
AGAAAATTCC TTGAAACATC AGATGATGAT TCTGTACAAG ATGACACAAT CATCAATGGT 
GAAATAATGA AAGGCATTGC AAATTCAACT GCATTATCTG ACATTACACT CGTATCTTCT 
GCAATTGATA ATATAACATT TGATACCTCT ATTTCAAGTA CTCATACTGA AAATATTACA 
CAAATGTTAG ATGTATCTTC AAAATCGATA CAATCCCATA CTCAATCTGA ATCTGCAATA 
TACAGAAAGA ATGAAAACAA GGAGAATAAT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100648332 ( XM_012313322.2 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_012168712.1 Bombus terrestris kinesin-like protein Klp61F (LOC100648332), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo59855
Clone ID Related Accession (Same CDS sequence)	XM_012313322.2
Accession Version	XM_012313322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	kinesin-like protein Klp61F isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_012313322.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGATA CACGGAGTGG AAGAAAGGAA AAATGTCAGC AGATACAAGT ATTTGTTCGT 
GTTAGACCTA TCAACAATTT TGAGGAAAAT AATAAATCTA CATCCATATT AGAAGTTCCA 
AATAATAGGG AAATAATAGT ACATGAACGT CCAAATGACA AAGTTTCTAA AAAATTTAAG 
TTTAATAATG TATTTGGACC TTTGTCAAAA CAGATCGATG TATATAATGT CGTTGTTAGT 
CCATTATTGG AACAAGTATT GGCTGGATAT AATTGTACAG TATTTGCATA TGGTCAAACT 
GGTACAGGGA AGACATACAC AATGGAAGGA ATTAACAATG ATCGAACATT ATATTGGCAC 
AGTGACTCGT CTGCTGGCAT GATACCACGT TCTTTAAGTC ACTTATTTGA CAAATTGCAA 
CTACTGGAAA CACAGGAATA TACAATTAGA GTTAGTTTTC TTGAACTCTA TAATGAAGAC 
ATATTTGATC TTTTATCATC TAGTAATGAT GCATGTAAAT TGAGGCTATA TGAAGATGCA 
TCAAAGAAAG GTGCAGTAAT CATACATGGA TTAGAAGAAG TAACAATACA CAATGCAAGT 
GAAGTTTATA AAATTATTAA GAAAGGGTCT GACAGAAGGC AAACAGCTGC AACATTGATG 
AATACTCAAT CTAGCCGGTC CCATACTATA TTCTCAATTA CTATACATAT GAAAGAAAGT 
ACTGGTAATA CAGATGGTGA AGAGATTCTG AAAATGGGGA AATTAAATTT AGTGGATCTG 
GCTGGTAGTG AAAATATTGG AAGATCTGGC TCTGTTGATA GGAGAGCAAG AGAAGCTGGT 
AATATTAACC AGTCTTTATT AACTCTTGGC AGAGTTATAA CAGCTCTTGT TGAAAAAGCA 
CCTCATATAC CTTACCGGGA ATCTAAACTT ACAAGATTAC TGCAGGAATC ATTAGGTGGT 
CGGACAAAAA CGTCAATTAT CGCAACAGTA TCCCCTGCTA GTATTAACCT TGAGGAAACA 
TTATCAACGC TAGATTATGC ACATCGTGCA AAAAATATCA CAAATCGTCC AGAAATAAAT 
CAGAAACTTT CGAAACGAGA ATTTTTGAAA CAATATACAG AAGAAATTGA AAAGCTGCGA 
AGAGATTTAT CAGCTTCACG TGATAGAAAT GGTGTTTATC TTGCAGATGA TAATTATAGA 
GAAATGGAAA CACTAATTTC ACGACAGACT AAGGAAATAG AAGAAAAAAT TAATCATATA 
AAAGCGCTTG AGAAAATTAT GCAAGACAAG GAGAAAATAT TTAATGAACT TGAATTACAA 
AATATTGCCC AGACAAAAGA ATTAGATGAA GTTAAGACTA AATTAAATAA TTTCTCTGAA 
GCTCTACAGT CGTCAAATTA TCGATTAAAA TTAACAACGC AGGAACGAGA TGAGCATAAA 
TATTTAGTAG AGAGACATAT GAATACAGAA CAATCTTTAT TGGATCAAGC TCAGACTTTG 
CTAAATGTTG CAGATACAGC AATAACAGAT TCATATAAAT TACACGATAA AATTGACAGG 
AAAAGTAAAA CAGAACAAAA ATTTGAAGAC CTTGGTAAGA CCGTGAAAAG TAATGTTTGT 
GAATGTTTGC AAAATATAGA AAAAAATATA TCTACATATG GTGAAACATT AAAGAGATTT 
TTTCCAGCAA TGAAAAATGA CATTGAAACC TCTATCAAAT GTAACGGTAT TGACACGATA 
ATACATCGAA ATGCGACGAG CAATTCGAAT CAATCAGATA CTACTAATTT AACAAAAAAT 
ATAAATGAAT TTCATTTAGA GTATCAGAAT TGGATACAAA ATGAAGTAAG AAATGTGGTT 
GGCACAGTGG AATCTGAACA TGAATTAATG AGTATCGTTT CTCTGCAATT GGCTGAAAAC 
ATCAACAGTA TGATAGAAAA TGAAATAGCT AAAAACATGG AAATCATAAG GAATAATGTA 
TCTGAAAAAT TAAAACAAAC TTCAACTCTT CTAAAAGAAA TGATAGATGC TTCTTGTAAT 
TATGAATTAG AAATTAATGA TCGTATGAGC CAAAGTGTCA AAAGTATTAT GGAAGCTGTT 
GAAGTACTTC GTAAAAAACA GGAATTTAGT GAAAAACAAC AGTTATTTAC TAAAATGATG 
GGAGATCTCT TTTCACAGTC AAATGAATTA CATAATAGTC AAGGAAAGCA TTATTCTGCT 
ACAATTGACA AATGTGATTA TGTTAGCAAA ATTTGTAATG AAGCTAATGT TCAAGCAACA 
AATAGTTCTA ATATAAATGT TAAAATGAGA AATGATTTAA AAGATTATAT ACAAAGGGAT 
ATAGAGAAAA TTGAAAATAA CGTTATTGTT ACAACAAGAC AGAATGAAGA AATTGCAAAT 
GAGATTACAA AGCGAGGAGA GGATTTAATA AACGAATTAA AGTCAAATAT AAATGAAAGT 
TGTAATATGT CGAAGCAGTA TATAAATGAT ATGGAATGCA ATATTAAGGA AAAGCAACTG 
AAAATGGATG CAGATAAGAA TATATCCCTT TCATTAATCA ATGATGCACA TGAAGTAATT 
TCAGATATAA GTAAAAACCA AGCAAGATTC GTGAAAGATA TGAAGGAAAA TACTGGATAT 
GTGTCTCAAG ACGTATCTGC CCAATTAGAA AACCAAATAA GGGAATCAAG TACACGGAAT 
GATAATATTA TTGAACAGTT ACAAATAACA GTTGGTAAAA TTAACAAATT TTTCGATGAA 
GATTTGCAAC GTGATGTCGC AACAGGATTA ACTCCTGTAA GGAAAGACTT TTCCTATCCT 
CGTCAATTTA AGGTAACATC TCCACATGAG CAAATTCTTA AAAAGTTTAG AGAAAGCAGA 
AAATTCCTTG AAACATCAGA TGATGATTCT GTACAAGATG ACACAATCAT CAATGGTGAA 
ATAATGAAAG GCATTGCAAA TTCAACTGCA TTATCTGACA TTACACTCGT ATCTTCTGCA 
ATTGATAATA TAACATTTGA TACCTCTATT TCAAGTACTC ATACTGAAAA TATTACACAA 
ATGTTAGATG TATCTTCAAA ATCGATACAA TCCCATACTC AATCTGAATC TGCAATATAC 
AGAAAGAATG AAAACAAGGA GAATAATAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012168712.1
CDS	196..3348
Translation	MNDTRSGRKEKCQQIQVFVRVRPINNFEENNKSTSILEVPNNREIIVHERPNDKVSKKFKFNNVFGPLSKQIDVYNVVVSPLLEQVLAGYNCTVFAYGQTGTGKTYTMEGINNDRTLYWHSDSSAGMIPRSLSHLFDKLQLLETQEYTIRVSFLELYNEDIFDLLSSSNDACKLRLYEDASKKGAVIIHGLEEVTIHNASEVYKIIKKGSDRRQTAATLMNTQSSRSHTIFSITIHMKESTGNTDGEEILKMGKLNLVDLAGSENIGRSGSVDRRAREAGNINQSLLTLGRVITALVEKAPHIPYRESKLTRLLQESLGGRTKTSIIATVSPASINLEETLSTLDYAHRAKNITNRPEINQKLSKREFLKQYTEEIEKLRRDLSASRDRNGVYLADDNYREMETLISRQTKEIEEKINHIKALEKIMQDKEKIFNELELQNIAQTKELDEVKTKLNNFSEALQSSNYRLKLTTQERDEHKYLVERHMNTEQSLLDQAQTLLNVADTAITDSYKLHDKIDRKSKTEQKFEDLGKTVKSNVCECLQNIEKNISTYGETLKRFFPAMKNDIETSIKCNGIDTIIHRNATSNSNQSDTTNLTKNINEFHLEYQNWIQNEVRNVVGTVESEHELMSIVSLQLAENINSMIENEIAKNMEIIRNNVSEKLKQTSTLLKEMIDASCNYELEINDRMSQSVKSIMEAVEVLRKKQEFSEKQQLFTKMMGDLFSQSNELHNSQGKHYSATIDKCDYVSKICNEANVQATNSSNINVKMRNDLKDYIQRDIEKIENNVIVTTRQNEEIANEITKRGEDLINELKSNINESCNMSKQYINDMECNIKEKQLKMDADKNISLSLINDAHEVISDISKNQARFVKDMKENTGYVSQDVSAQLENQIRESSTRNDNIIEQLQITVGKINKFFDEDLQRDVATGLTPVRKDFSYPRQFKVTSPHEQILKKFRESRKFLETSDDDSVQDDTIINGEIMKGIANSTALSDITLVSSAIDNITFDTSISSTHTENITQMLDVSSKSIQSHTQSESAIYRKNENKENNN

Target ORF information:

RefSeq Version	XM_012313322.2
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris kinesin-like protein Klp61F (LOC100648332), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012313322.2

ORF Insert Sequence:

ATGAACGATA CACGGAGTGG AAGAAAGGAA AAATGTCAGC AGATACAAGT ATTTGTTCGT 
GTTAGACCTA TCAACAATTT TGAGGAAAAT AATAAATCTA CATCCATATT AGAAGTTCCA 
AATAATAGGG AAATAATAGT ACATGAACGT CCAAATGACA AAGTTTCTAA AAAATTTAAG 
TTTAATAATG TATTTGGACC TTTGTCAAAA CAGATCGATG TATATAATGT CGTTGTTAGT 
CCATTATTGG AACAAGTATT GGCTGGATAT AATTGTACAG TATTTGCATA TGGTCAAACT 
GGTACAGGGA AGACATACAC AATGGAAGGA ATTAACAATG ATCGAACATT ATATTGGCAC 
AGTGACTCGT CTGCTGGCAT GATACCACGT TCTTTAAGTC ACTTATTTGA CAAATTGCAA 
CTACTGGAAA CACAGGAATA TACAATTAGA GTTAGTTTTC TTGAACTCTA TAATGAAGAC 
ATATTTGATC TTTTATCATC TAGTAATGAT GCATGTAAAT TGAGGCTATA TGAAGATGCA 
TCAAAGAAAG GTGCAGTAAT CATACATGGA TTAGAAGAAG TAACAATACA CAATGCAAGT 
GAAGTTTATA AAATTATTAA GAAAGGGTCT GACAGAAGGC AAACAGCTGC AACATTGATG 
AATACTCAAT CTAGCCGGTC CCATACTATA TTCTCAATTA CTATACATAT GAAAGAAAGT 
ACTGGTAATA CAGATGGTGA AGAGATTCTG AAAATGGGGA AATTAAATTT AGTGGATCTG 
GCTGGTAGTG AAAATATTGG AAGATCTGGC TCTGTTGATA GGAGAGCAAG AGAAGCTGGT 
AATATTAACC AGTCTTTATT AACTCTTGGC AGAGTTATAA CAGCTCTTGT TGAAAAAGCA 
CCTCATATAC CTTACCGGGA ATCTAAACTT ACAAGATTAC TGCAGGAATC ATTAGGTGGT 
CGGACAAAAA CGTCAATTAT CGCAACAGTA TCCCCTGCTA GTATTAACCT TGAGGAAACA 
TTATCAACGC TAGATTATGC ACATCGTGCA AAAAATATCA CAAATCGTCC AGAAATAAAT 
CAGAAACTTT CGAAACGAGA ATTTTTGAAA CAATATACAG AAGAAATTGA AAAGCTGCGA 
AGAGATTTAT CAGCTTCACG TGATAGAAAT GGTGTTTATC TTGCAGATGA TAATTATAGA 
GAAATGGAAA CACTAATTTC ACGACAGACT AAGGAAATAG AAGAAAAAAT TAATCATATA 
AAAGCGCTTG AGAAAATTAT GCAAGACAAG GAGAAAATAT TTAATGAACT TGAATTACAA 
AATATTGCCC AGACAAAAGA ATTAGATGAA GTTAAGACTA AATTAAATAA TTTCTCTGAA 
GCTCTACAGT CGTCAAATTA TCGATTAAAA TTAACAACGC AGGAACGAGA TGAGCATAAA 
TATTTAGTAG AGAGACATAT GAATACAGAA CAATCTTTAT TGGATCAAGC TCAGACTTTG 
CTAAATGTTG CAGATACAGC AATAACAGAT TCATATAAAT TACACGATAA AATTGACAGG 
AAAAGTAAAA CAGAACAAAA ATTTGAAGAC CTTGGTAAGA CCGTGAAAAG TAATGTTTGT 
GAATGTTTGC AAAATATAGA AAAAAATATA TCTACATATG GTGAAACATT AAAGAGATTT 
TTTCCAGCAA TGAAAAATGA CATTGAAACC TCTATCAAAT GTAACGGTAT TGACACGATA 
ATACATCGAA ATGCGACGAG CAATTCGAAT CAATCAGATA CTACTAATTT AACAAAAAAT 
ATAAATGAAT TTCATTTAGA GTATCAGAAT TGGATACAAA ATGAAGTAAG AAATGTGGTT 
GGCACAGTGG AATCTGAACA TGAATTAATG AGTATCGTTT CTCTGCAATT GGCTGAAAAC 
ATCAACAGTA TGATAGAAAA TGAAATAGCT AAAAACATGG AAATCATAAG GAATAATGTA 
TCTGAAAAAT TAAAACAAAC TTCAACTCTT CTAAAAGAAA TGATAGATGC TTCTTGTAAT 
TATGAATTAG AAATTAATGA TCGTATGAGC CAAAGTGTCA AAAGTATTAT GGAAGCTGTT 
GAAGTACTTC GTAAAAAACA GGAATTTAGT GAAAAACAAC AGTTATTTAC TAAAATGATG 
GGAGATCTCT TTTCACAGTC AAATGAATTA CATAATAGTC AAGGAAAGCA TTATTCTGCT 
ACAATTGACA AATGTGATTA TGTTAGCAAA ATTTGTAATG AAGCTAATGT TCAAGCAACA 
AATAGTTCTA ATATAAATGT TAAAATGAGA AATGATTTAA AAGATTATAT ACAAAGGGAT 
ATAGAGAAAA TTGAAAATAA CGTTATTGTT ACAACAAGAC AGAATGAAGA AATTGCAAAT 
GAGATTACAA AGCGAGGAGA GGATTTAATA AACGAATTAA AGTCAAATAT AAATGAAAGT 
TGTAATATGT CGAAGCAGTA TATAAATGAT ATGGAATGCA ATATTAAGGA AAAGCAACTG 
AAAATGGATG CAGATAAGAA TATATCCCTT TCATTAATCA ATGATGCACA TGAAGTAATT 
TCAGATATAA GTAAAAACCA AGCAAGATTC GTGAAAGATA TGAAGGAAAA TACTGGATAT 
GTGTCTCAAG ACGTATCTGC CCAATTAGAA AACCAAATAA GGGAATCAAG TACACGGAAT 
GATAATATTA TTGAACAGTT ACAAATAACA GTTGGTAAAA TTAACAAATT TTTCGATGAA 
GATTTGCAAC GTGATGTCGC AACAGGATTA ACTCCTGTAA GGAAAGACTT TTCCTATCCT 
CGTCAATTTA AGGTAACATC TCCACATGAG CAAATTCTTA AAAAGTTTAG AGAAAGCAGA 
AAATTCCTTG AAACATCAGA TGATGATTCT GTACAAGATG ACACAATCAT CAATGGTGAA 
ATAATGAAAG GCATTGCAAA TTCAACTGCA TTATCTGACA TTACACTCGT ATCTTCTGCA 
ATTGATAATA TAACATTTGA TACCTCTATT TCAAGTACTC ATACTGAAAA TATTACACAA 
ATGTTAGATG TATCTTCAAA ATCGATACAA TCCCATACTC AATCTGAATC TGCAATATAC 
AGAAAGAATG AAAACAAGGA GAATAATAAC TAA