LOC100646512 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100646512
Entrez Gene ID	100646512
Full Name	nuclear pore complex protein Nup133
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003399042.3	XP_003399090.1	nuclear pore complex protein Nup133

Pathways from BioSystems
Gene Ontology

The following LOC100646512 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100646512 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo60161	XM_003399042.3 Latest version!	Bombus terrestris nuclear pore complex protein Nup133 (LOC100646512), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100646512 ( XM_003399042.3 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_003399090.1 Bombus terrestris nuclear pore complex protein Nup133 (LOC100646512), mRNA.

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CloneID	OBo60161
Clone ID Related Accession (Same CDS sequence)	XM_003399042.3
Accession Version	XM_003399042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	nuclear pore complex protein Nup133
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015772.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_003399042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCGTA CGAGTATAGG AAATTCACTA GGAAGAAATT TGACGTCTCC GCGAAAACGC 
ATGTCAATCG TTCAATCCTT AAGAAAAAAT AATTCAGCGA TAAGTGTATC CGGACGATCG 
AATCAGTCAG TACAGATAAT ATGTAAAACG CCGAATCATG TTGTAGAAAG TTTCGGATCC 
TCCTTGCCTG TACTTATTAC GGAAGCTTTA ACATTTGTAG ATAGAAACAC AGCTGTTAGC 
GTTAATATCT CAATAGATGG TTGGGCTTGG CTTGTATGTG GTCGTAGACT GCTTGTGTGG 
CAATGTAAGG CCACTATCCA TGATTCAAAA CAAAGGAGAA CTTTTAAGAG CCAATGCAGA 
GAGTTATTGT TACCTCAAAG TGATTTAGCT CACAAAGCAG AATGCATAGC TGTGTGGTTG 
CCTCCAGGCC ATCAGGTGCC TAGTTGCATG GCTGTTTCTC CTGAAGGTAT TGTACGGTTC 
TGGGTTAGCG TTGCCCATGA AGGATCTTCT GTAGAAACAA GTGCAGAACT TGCTGGGCAA 
GAAGTCGATT GTCTTACTTA TATTCCTGGT CATGGTTGTA TTTTAGCTAC AACCACATGT 
ACAGTAGCAT TGTTACAACC ACAGTTTGTA GGTGGTAAGA ATAGTATCAA CTGTCAAGTT 
CTTAGAACAA GTCAAGGATG GCTAGGTGGT ATAGGAAGAA GAATGACTTC TCTTATATTT 
GGAGCTATAC CTCAATCTCC AGTCACAGAA ACTAAATTAG TAAAAGTTAC TTGCACAACC 
TTAGGAGATA GAGGATCAAG GGTTCTTATT TTAGCTGGCT CATCTTTGCA ATATTGGTCT 
TTCCCACATA ATGAGCAAGA GAAGATGGAG TTTGATGAAG ATATTGGGTA CATTATTTCA 
CAAACTTTCC AACGAGAAAT TTGGAAATCA AGTGCATGCA ACCCACAAAA TATGGAAACC 
TGGTTGATTG ATATGCAACC ATGTAATGAA GGAATAGTTT TTTTAATGGC AGCCCATTGT 
GCCGATGCAT CACCTCTTAT TGAATTTGCA CTTGGTTTGA TACAGCTATC TGGTATAACG 
TTAGCAAATA CATTTAAATG GTTTATACCA GTTAAAATTG ATTCCATTTC GTATCATAAT 
GATGTTGAAT CAACTTTAGT TTCTTACCAT TTTATACTAC ATGGTTGGGA AGCCATTATA 
TACAATCAGT ATGAAGTGCT TGTTGTGAAC TGTATATCTG AGCACGAGCA AGATAAAATA 
GATTTAGTGC GAGGCGGAGA AGATAGTATT CTTGGTGGTG CACTATGTTC AGGAACTCCA 
GTTTTATTTA CTAAGAATTT CGGATTAGTT TCCGTCATAC CGTCGGATTT TATATCGCAA 
GATTTTAATA TGAGTTATAC AGACAATGTT AATACAAGTG TGGATTATAA TTATCGACCT 
AGTTCAGCTG CACAAAATTT TAACAGTCTT ATCAGTAATG AAGAAATTCA AGATATGTAT 
TACAGTTCTG ATTGTGCTAC ACAATTGCGT GCTGCCTTTC TCCTTAGTTT ACGGCAAAGT 
GAGGCTCAGT GTGAAGATAT TTTATTACAA CTCTTTCCTT TGCAAGAAGA ACCAGTAATG 
GATATAGATG CTAATCTCGA CACACTGATT TTAAAAGTTG CTAGAGACCT GATAGATGAT 
TATCCAGCAA ATGATCCACG TTGGTCAAAT CACAGAGATT TGTCTATGAC GATAAATGCA 
GTCACTTCTA TGCAAATACC TAATCAACTA GAAGGGAAAC AGAAAGCCAT AGATTTATTT 
ACAACATTTC TAAAAGAACA TGATCTATGG AACAGATTCT GTGCTGTTAC ATATAGAGGA 
CTAATTATGT CTACACCACA CGTTCTTGCA GAATATGCAG AAAAGATAGT TGCAGCACTA 
ACTATATATA ATCTCCAGAA CAAGTATACT GATATTATAG ATACTATAAT AGAACAAACT 
TTAAGCCCTG AAGCTTACAT ATCTGACGAA TTAACAGCGC GTGATATATT TTATCGTCAA 
GTCAGTACTG TACATCGTTT TCTCCCTACT TTAGTAAACA ATGCATCAGA AGTAACTCAA 
TCCGAAAGAC CCATCCAACA AGTAGCACAT TATATTATGC AAGTAAACGC TATATTATTA 
GGTATACTAC ATGAAGTGGT GAAATACCGG CAACATAATG CTGAACGATT TATTCCTACG 
AGATGTTCGA ACGGAATAAC CGAGTATCTT CCGTGGACAG CTGCAGTTGG AAAACATGGA 
TTAAGAAATT GTCTCAACAC AATGTATAAT GTAACTCTCA AGCATGGCAT AACTGGAAGT 
AACGATTCAA CAGTGAGAAA TGAATTATAT GAACAATTAG TAAGTTATAT AGACTTAATA 
TTAGATGGCC GAAAATGTCA TTTAGAAAGT GTTAAGGGTA CAGAAAAGTT TGAAATACTT 
TTGAAGCAAT ACGAAACAGA TCGAATGAAT CTAATTCAAC CTCTAATAAA AGAAGAACAA 
TACGATAGTG CTGCAATGTT AGCTGAAAAG TATTGCGATT TTGCGTCCCT GATACAGATA 
TGCGAATTAA CTAACAATAA AAGCCAACTG GATGGATATA TGAAAAAATT TGCAGCTCAA 
GATTTCGTAG GATTTTTATT CTCATGGTAC GTGAAAGATG GTCGCCAGGG TCAGTTAGTA 
GAGAAATGTA GACGCGGAGG TACCATTGAA TTGTCAGAAA AGTTGGCAAA ACATCCAACT 
CTATCATGGG TACAATCTGC TCTCACGGAC GAGTTACGCT TTGCTGCTAA TACGCTTTAT 
TCTTTAGCAA TTCAAGAAAG TGAATTAGTG ACACGTAAGA AGTCCATACT TTCTTTAGCA 
AAATTGGCCC TTCTTGCTTC CGATGATTTA GAAGAAGAAG TGAAAGATTG TGTAAAAAGA 
ATCAATAATG AATTGGCGCT AGTAGCTTAT CAAGAAGAAT TACCAACTCA AGTACTTACT 
ACATATGGTT ATGATGTAGA AAAATTACAA GTATTTACAC CAACTGAATT AATTACGTTG 
TACACATCTG AGGATAATAT TGATGCAAAT GAGTATGACT TCAAGAAAGC TCTTGATTTG 
CTTGAGTATG TGGAGCAAGA AGATGAAAAA GTGTCATTGA AATTGCAAAT TTGGGCACGA 
GCTGCTAAAC GGGATCAGTG GGATACCCTA GGAAAGAATC CTGAACAACA AGTACAAGAA 
ACAATATTTT TCAAATTAAT GGACCTTGCA CATTTTACTG GTGATCAAGT AAACGAATTT 
CTTCCGCCAG TTGATATGCT TTTGGTAGAA CCTGAATTGG GAGATCTTGC TGCATCAAGC 
AACTTTCAAT TCCTCATCAA ATTTGTGTAT GAGTATGCAT ATAGCAATTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003399090.1
CDS	376..3789
Translation	MDRTSIGNSLGRNLTSPRKRMSIVQSLRKNNSAISVSGRSNQSVQIICKTPNHVVESFGSSLPVLITEALTFVDRNTAVSVNISIDGWAWLVCGRRLLVWQCKATIHDSKQRRTFKSQCRELLLPQSDLAHKAECIAVWLPPGHQVPSCMAVSPEGIVRFWVSVAHEGSSVETSAELAGQEVDCLTYIPGHGCILATTTCTVALLQPQFVGGKNSINCQVLRTSQGWLGGIGRRMTSLIFGAIPQSPVTETKLVKVTCTTLGDRGSRVLILAGSSLQYWSFPHNEQEKMEFDEDIGYIISQTFQREIWKSSACNPQNMETWLIDMQPCNEGIVFLMAAHCADASPLIEFALGLIQLSGITLANTFKWFIPVKIDSISYHNDVESTLVSYHFILHGWEAIIYNQYEVLVVNCISEHEQDKIDLVRGGEDSILGGALCSGTPVLFTKNFGLVSVIPSDFISQDFNMSYTDNVNTSVDYNYRPSSAAQNFNSLISNEEIQDMYYSSDCATQLRAAFLLSLRQSEAQCEDILLQLFPLQEEPVMDIDANLDTLILKVARDLIDDYPANDPRWSNHRDLSMTINAVTSMQIPNQLEGKQKAIDLFTTFLKEHDLWNRFCAVTYRGLIMSTPHVLAEYAEKIVAALTIYNLQNKYTDIIDTIIEQTLSPEAYISDELTARDIFYRQVSTVHRFLPTLVNNASEVTQSERPIQQVAHYIMQVNAILLGILHEVVKYRQHNAERFIPTRCSNGITEYLPWTAAVGKHGLRNCLNTMYNVTLKHGITGSNDSTVRNELYEQLVSYIDLILDGRKCHLESVKGTEKFEILLKQYETDRMNLIQPLIKEEQYDSAAMLAEKYCDFASLIQICELTNNKSQLDGYMKKFAAQDFVGFLFSWYVKDGRQGQLVEKCRRGGTIELSEKLAKHPTLSWVQSALTDELRFAANTLYSLAIQESELVTRKKSILSLAKLALLASDDLEEEVKDCVKRINNELALVAYQEELPTQVLTTYGYDVEKLQVFTPTELITLYTSEDNIDANEYDFKKALDLLEYVEQEDEKVSLKLQIWARAAKRDQWDTLGKNPEQQVQETIFFKLMDLAHFTGDQVNEFLPPVDMLLVEPELGDLAASSNFQFLIKFVYEYAYSNY

Target ORF information:

RefSeq Version	XM_003399042.3
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris nuclear pore complex protein Nup133 (LOC100646512), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003399042.3

ORF Insert Sequence:

ATGGATCGTA CGAGTATAGG AAATTCACTA GGAAGAAATT TGACGTCTCC GCGAAAACGC 
ATGTCAATCG TTCAATCCTT AAGAAAAAAT AATTCAGCGA TAAGTGTATC CGGACGATCG 
AATCAGTCAG TACAGATAAT ATGTAAAACG CCGAATCATG TTGTAGAAAG TTTCGGATCC 
TCCTTGCCTG TACTTATTAC GGAAGCTTTA ACATTTGTAG ATAGAAACAC AGCTGTTAGC 
GTTAATATCT CAATAGATGG TTGGGCTTGG CTTGTATGTG GTCGTAGACT GCTTGTGTGG 
CAATGTAAGG CCACTATCCA TGATTCAAAA CAAAGGAGAA CTTTTAAGAG CCAATGCAGA 
GAGTTATTGT TACCTCAAAG TGATTTAGCT CACAAAGCAG AATGCATAGC TGTGTGGTTG 
CCTCCAGGCC ATCAGGTGCC TAGTTGCATG GCTGTTTCTC CTGAAGGTAT TGTACGGTTC 
TGGGTTAGCG TTGCCCATGA AGGATCTTCT GTAGAAACAA GTGCAGAACT TGCTGGGCAA 
GAAGTCGATT GTCTTACTTA TATTCCTGGT CATGGTTGTA TTTTAGCTAC AACCACATGT 
ACAGTAGCAT TGTTACAACC ACAGTTTGTA GGTGGTAAGA ATAGTATCAA CTGTCAAGTT 
CTTAGAACAA GTCAAGGATG GCTAGGTGGT ATAGGAAGAA GAATGACTTC TCTTATATTT 
GGAGCTATAC CTCAATCTCC AGTCACAGAA ACTAAATTAG TAAAAGTTAC TTGCACAACC 
TTAGGAGATA GAGGATCAAG GGTTCTTATT TTAGCTGGCT CATCTTTGCA ATATTGGTCT 
TTCCCACATA ATGAGCAAGA GAAGATGGAG TTTGATGAAG ATATTGGGTA CATTATTTCA 
CAAACTTTCC AACGAGAAAT TTGGAAATCA AGTGCATGCA ACCCACAAAA TATGGAAACC 
TGGTTGATTG ATATGCAACC ATGTAATGAA GGAATAGTTT TTTTAATGGC AGCCCATTGT 
GCCGATGCAT CACCTCTTAT TGAATTTGCA CTTGGTTTGA TACAGCTATC TGGTATAACG 
TTAGCAAATA CATTTAAATG GTTTATACCA GTTAAAATTG ATTCCATTTC GTATCATAAT 
GATGTTGAAT CAACTTTAGT TTCTTACCAT TTTATACTAC ATGGTTGGGA AGCCATTATA 
TACAATCAGT ATGAAGTGCT TGTTGTGAAC TGTATATCTG AGCACGAGCA AGATAAAATA 
GATTTAGTGC GAGGCGGAGA AGATAGTATT CTTGGTGGTG CACTATGTTC AGGAACTCCA 
GTTTTATTTA CTAAGAATTT CGGATTAGTT TCCGTCATAC CGTCGGATTT TATATCGCAA 
GATTTTAATA TGAGTTATAC AGACAATGTT AATACAAGTG TGGATTATAA TTATCGACCT 
AGTTCAGCTG CACAAAATTT TAACAGTCTT ATCAGTAATG AAGAAATTCA AGATATGTAT 
TACAGTTCTG ATTGTGCTAC ACAATTGCGT GCTGCCTTTC TCCTTAGTTT ACGGCAAAGT 
GAGGCTCAGT GTGAAGATAT TTTATTACAA CTCTTTCCTT TGCAAGAAGA ACCAGTAATG 
GATATAGATG CTAATCTCGA CACACTGATT TTAAAAGTTG CTAGAGACCT GATAGATGAT 
TATCCAGCAA ATGATCCACG TTGGTCAAAT CACAGAGATT TGTCTATGAC GATAAATGCA 
GTCACTTCTA TGCAAATACC TAATCAACTA GAAGGGAAAC AGAAAGCCAT AGATTTATTT 
ACAACATTTC TAAAAGAACA TGATCTATGG AACAGATTCT GTGCTGTTAC ATATAGAGGA 
CTAATTATGT CTACACCACA CGTTCTTGCA GAATATGCAG AAAAGATAGT TGCAGCACTA 
ACTATATATA ATCTCCAGAA CAAGTATACT GATATTATAG ATACTATAAT AGAACAAACT 
TTAAGCCCTG AAGCTTACAT ATCTGACGAA TTAACAGCGC GTGATATATT TTATCGTCAA 
GTCAGTACTG TACATCGTTT TCTCCCTACT TTAGTAAACA ATGCATCAGA AGTAACTCAA 
TCCGAAAGAC CCATCCAACA AGTAGCACAT TATATTATGC AAGTAAACGC TATATTATTA 
GGTATACTAC ATGAAGTGGT GAAATACCGG CAACATAATG CTGAACGATT TATTCCTACG 
AGATGTTCGA ACGGAATAAC CGAGTATCTT CCGTGGACAG CTGCAGTTGG AAAACATGGA 
TTAAGAAATT GTCTCAACAC AATGTATAAT GTAACTCTCA AGCATGGCAT AACTGGAAGT 
AACGATTCAA CAGTGAGAAA TGAATTATAT GAACAATTAG TAAGTTATAT AGACTTAATA 
TTAGATGGCC GAAAATGTCA TTTAGAAAGT GTTAAGGGTA CAGAAAAGTT TGAAATACTT 
TTGAAGCAAT ACGAAACAGA TCGAATGAAT CTAATTCAAC CTCTAATAAA AGAAGAACAA 
TACGATAGTG CTGCAATGTT AGCTGAAAAG TATTGCGATT TTGCGTCCCT GATACAGATA 
TGCGAATTAA CTAACAATAA AAGCCAACTG GATGGATATA TGAAAAAATT TGCAGCTCAA 
GATTTCGTAG GATTTTTATT CTCATGGTAC GTGAAAGATG GTCGCCAGGG TCAGTTAGTA 
GAGAAATGTA GACGCGGAGG TACCATTGAA TTGTCAGAAA AGTTGGCAAA ACATCCAACT 
CTATCATGGG TACAATCTGC TCTCACGGAC GAGTTACGCT TTGCTGCTAA TACGCTTTAT 
TCTTTAGCAA TTCAAGAAAG TGAATTAGTG ACACGTAAGA AGTCCATACT TTCTTTAGCA 
AAATTGGCCC TTCTTGCTTC CGATGATTTA GAAGAAGAAG TGAAAGATTG TGTAAAAAGA 
ATCAATAATG AATTGGCGCT AGTAGCTTAT CAAGAAGAAT TACCAACTCA AGTACTTACT 
ACATATGGTT ATGATGTAGA AAAATTACAA GTATTTACAC CAACTGAATT AATTACGTTG 
TACACATCTG AGGATAATAT TGATGCAAAT GAGTATGACT TCAAGAAAGC TCTTGATTTG 
CTTGAGTATG TGGAGCAAGA AGATGAAAAA GTGTCATTGA AATTGCAAAT TTGGGCACGA 
GCTGCTAAAC GGGATCAGTG GGATACCCTA GGAAAGAATC CTGAACAACA AGTACAAGAA 
ACAATATTTT TCAAATTAAT GGACCTTGCA CATTTTACTG GTGATCAAGT AAACGAATTT 
CTTCCGCCAG TTGATATGCT TTTGGTAGAA CCTGAATTGG GAGATCTTGC TGCATCAAGC 
AACTTTCAAT TCCTCATCAA ATTTGTGTAT GAGTATGCAT ATAGCAATTA TTAA