LOC100643170 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100643170
Entrez Gene ID	100643170
Full Name	trimethylguanosine synthase
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012312366.2	XP_012167756.2	LOW QUALITY PROTEIN: trimethylguanosine synthase

Pathways from BioSystems
Gene Ontology

The following LOC100643170 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100643170 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo58851	XM_012312366.2 Latest version!	Bombus terrestris trimethylguanosine synthase (LOC100643170), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100643170 ( XM_012312366.2 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_012167756.2 Bombus terrestris trimethylguanosine synthase (LOC100643170), mRNA.

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CloneID	OBo58851
Clone ID Related Accession (Same CDS sequence)	XM_012312366.2
Accession Version	XM_012312366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	LOW QUALITY PROTEIN: trimethylguanosine synthase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015771.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 25, 2017 this sequence version replaced XM_012312366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAGTGACC GCTTTTGGGA ACCTTTAGCC GAAGTGTACA TCGCAAAACA AGCTGAGTCT 
ACTGACCCTG ATCATTACAT TTATTGTCTT TGCAGCAGAG TATTTATTCG ACACCCAGAA 
GTTTATACTG CCCTTGCTCT AGAAGATGCT AGTAAAGATG AAGATATTGA GAGCGATTGT 
GTTGGAGAAA TGTTAGACCT CCATAAAAGG TCTATTATAG AAACACAATC CATAGAGAAA 
CATGATGAAG AACCAGTGAG CTGTTACTGC AGTGCATCAC ACACAGACAA CAATTATTCC 
ACAGATGAAC ATGACTCTGT TCGTGATTCG GCTCATCGTC CTACTGCACA TTCCCTCACG 
CAAAAATTGG GCCTGCACCA GTCAGATTCA GGTGCAGACC TATCAGAATA TCATGATCAA 
CATGAGGCTA AAAGCATACA AAATCTATTG GCATCTTATG GGAAAACTTT TCAAAATACA 
GAGACTGAAC TGGAAGATGA ATTTGACAAA GTGAATGTAA TTGCACAGCA CCAAGAAGAT 
AAAGATGTAG AATTTGAATG TAAAATAACA AACATTCACT CATCAGATCA TCTTGCAGAT 
AACATTGAAA TTTTAAGTTA TGATCCATCC ATTTGTAATG AAAAGGAACT TCACATAGAA 
AATCCATCAT GTATTTTAGA AGACAAAGAT TTTGAGATAC ATAATATATT TAAAAATGTT 
TCTTTGAATG ATACACAAAC TTATAAGGAA GATGTCCAAC TCAAGTACAA CTGTGACCTT 
AGCTCGTGTG ACTATCCATA TTATTCTATG TATTCTAAAA CTAAGAGTAT AATAGATATG 
GCATGGGAGA AATATTGGTC TAAAAATGGT GAACGTATAA TTTGGACAAC TTGGATTAGG 
AAATATGCAG ATTATATAAA TCCAGAATAT TTGCAACAAA ATGCTCATTT AATAAGTAAT 
GAAAAGTTAG AAGTGAAAGA TACTATCGAA AAATTTTCAG AGCAAAATAC ATGTTTTCCT 
AGTCAAGCGC ACAGAAATTG TGAACTTGAT CGCTCAAATT TTGAGGGAAT ATTTAGCAAA 
AGTAATAATG CTGGTAATAA TGAAATAAAA GCAAAAGACA CGTGTAGTAT TAATTTTTCA 
TTTGATGACA TAAGCAAGCA AGAGATAAGT GACAAAGATG CGGAAGATAA TAGAAAGAAA 
TTGACAAATT TTGAAGCATC ACTGGAAACT GGAGATGGTT GGAATCCATT AAGTCCTTTT 
AGTATGGAAG AAAGTTACAA CCAACAATCT AATGCGGAAG ACGAAAGACT TTTAACAAGG 
TGTGATTCTA TTAACGAGTC AATAGCAAAA ACAAATGCAA CATCAGATTC TATGACAAAC 
GTCACAAAGA TGACCCTTAC TAGTTCTAGT TGTGACTCTA TTTCTACAAA TTCATCTAGT 
CTCATCAGTT CTGTAACTAG TTCCATTGAA AGCAATATAA CAAACACAAG TTCTGATCAA 
GATAATGATC AGGAATATAC ACCAGAAGAT ACTGATAAGT ACTGGCAACA TTTATGGAAA 
GAAAATTTCC AAATAGAGTA CCAAAATCAT TATAAACTAT TTATAGAAAA ATATAAAAGG 
GAGCAACCTG AAATTAGCAA TCAAAGTTAT AGCCAGGAAT ATAATGATAT AGATATGAAA 
GAGGAAGGAA GTGATGAAAG TATAGACCAA GATTTTACAC AAAGGGAAAT AGACACATCT 
AATGAGATTA ATATTTCTGA AAGTAAAAGT GCACCTAGTA AAAATTTTTC TTATTCTAAG 
AAAGATAAGA CAAAAAAGAA ACGATTAATT ATGGAATCAG TGGGAATGCT CATTCAAAAT 
TTAACAGTAG AAGCAAAAGA TAATGAAACT GATGTACATA AAAATGAAAA AATTAATGAA 
ATGGAATGTT CACAAGAAGT TGAACATGAT CAAACTTCAA CTACAGAAAA TGAAAATGGT 
GCAATTAATT CCAATGATGC TAGTGGCAAC AATAGTATTC AACGTAAATA TTCTAATGAA 
GGAGATGGAG ATGAGCCCAA TGAAGATAAA CCTATAACAT TAAAACGGAG TCATGAGGCT 
GATTATGATG ACACGGAAGA AGGCCTGGAA TCAGTAAAAA AAGCATTTTC ATTGATGGGA 
TATACTTTCA ACGAAAATTA CGAACAATCT AATTTCCAAG GAGAAGTAGT ATATAGAAAA 
AAANATATTA GGTTACAAAA TAGACAATTA AAAATGAAAT ATAAACCCAA GCCAATAAAT 
AAACATATAT ACTTTGATGA TAACGGAGTG GAAATTACTA ACACTATAGA CAAAGTGAAG 
CATTATTTAT CATACTGTCC TGTTTTGCCA CCAGTAAAAA CAGAATTACA ATCCTCTGAA 
AAAGGTTCCT ACACAGCACA ATTAACGTCA AGTTCTGATG ATGAATGTGA TCATAGCCTT 
AAGTCAAAGT TACAAACAAA ACGATTTGTA TTTAGTAAAC CAAGCACAAG TTCAATAGAT 
TCAGGAGCAG ATAGAAAACA ATTTGATGAT GTTCACGATA CCTTAAACGT ATTTGATAAA 
CAGGGCAATG TTTTCCAAAG TATCAAAGAT GATAATATAT ACTTTGATCA GGATGAAACA 
AGCAATTCCA CAAAATGTGT ACCAGAAAAT CAAATTTTCG AACAAGAGAA CTTTGATGTA 
TTAAATGTCA ATATGGATAT AGATGAAAGC TATAGTAACA ACGAAATACA AAGTATGAAA 
TTATCAGATG AAGATAGCGT AAAGAAGACG CAAAAGAAAA AACGAAGGAA GCCACCCAAA 
AGAAATGTAA GCCTGCCACT AGAAATAGAC AATGATAAAA CTTTAATGAA ATATTGGTTC 
AAAAGATACC GCCTATTTAG CAAATTTGAT CAAGGCATTA AAATGGATCG TGAGAGCTGG 
TATTCTGTAA CACCAGAAAA AATTGCGGAG CACATAGCTC AAAGATGCAA ATGCGATACC 
GTAATCGATG CATTCTGTGG AGCAGGAGGG AACGCTATTC AATTTGCTTT TACATGTGAA 
AGAGTTTTCG CAATAGATAT AGATCCAGCT AAAATCGAAA TTGCTCGAAA TAATGCTCGA 
GTTTATGGCG TTGAAGACAG AATAGAATTC ATTGTTGGAG ATTTTATTAA ACTCGCATCA 
AAGTTAAGCG CAGATGTTGT GTTTCTAAGT CCTCCATGGG GAGGACCTGA ATATGCCAAG 
AATGAAACAT TTGATCTCAA TAATATAATG CTTCCCATTG GTGGTGTAAA TTTATTTAAT 
ATAGCAAGAA AAATCACAGA TCATGTGGCC TACTTTCTAC CTAAAAATGT AGATACAATG 
CAGCTTGCCA TGTTAGCCGC AGTAGGTGGT GGTGTAGAAG TGGAACAAAA CTTTCTTGAT 
AGAAAGTTAA TAGCTTTAAC AGCTTATTAT GGCGAACTGC CCAGAGACTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012167756.2
CDS	144..3617
Translation	MSDRFWEPLAEVYIAKQAESTDPDHYIYCLCSRVFIRHPEVYTALALEDASKDEDIESDCVGEMLDLHKRSIIETQSIEKHDEEPVSCYCSASHTDNNYSTDEHDSVRDSAHRPTAHSLTQKLGLHQSDSGADLSEYHDQHEAKSIQNLLASYGKTFQNTETELEDEFDKVNVIAQHQEDKDVEFECKITNIHSSDHLADNIEILSYDPSICNEKELHIENPSCILEDKDFEIHNIFKNVSLNDTQTYKEDVQLKYNCDLSSCDYPYYSMYSKTKSIIDMAWEKYWSKNGERIIWTTWIRKYADYINPEYLQQNAHLISNEKLEVKDTIEKFSEQNTCFPSQAHRNCELDRSNFEGIFSKSNNAGNNEIKAKDTCSINFSFDDISKQEISDKDAEDNRKKLTNFEASLETGDGWNPLSPFSMEESYNQQSNAEDERLLTRCDSINESIAKTNATSDSMTNVTKMTLTSSSCDSISTNSSSLISSVTSSIESNITNTSSDQDNDQEYTPEDTDKYWQHLWKENFQIEYQNHYKLFIEKYKREQPEISNQSYSQEYNDIDMKEEGSDESIDQDFTQREIDTSNEINISESKSAPSKNFSYSKKDKTKKKRLIMESVGMLIQNLTVEAKDNETDVHKNEKINEMECSQEVEHDQTSTTENENGAINSNDASGNNSIQRKYSNEGDGDEPNEDKPITLKRSHEADYDDTEEGLESVKKAFSLMGYTFNENYEQSNFQGEVVYRKKnullIRLQNRQLKMKYKPKPINKHIYFDDNGVEITNTIDKVKHYLSYCPVLPPVKTELQSSEKGSYTAQLTSSSDDECDHSLKSKLQTKRFVFSKPSTSSIDSGADRKQFDDVHDTLNVFDKQGNVFQSIKDDNIYFDQDETSNSTKCVPENQIFEQENFDVLNVNMDIDESYSNNEIQSMKLSDEDSVKKTQKKKRRKPPKRNVSLPLEIDNDKTLMKYWFKRYRLFSKFDQGIKMDRESWYSVTPEKIAEHIAQRCKCDTVIDAFCGAGGNAIQFAFTCERVFAIDIDPAKIEIARNNARVYGVEDRIEFIVGDFIKLASKLSADVVFLSPPWGGPEYAKNETFDLNNIMLPIGGVNLFNIARKITDHVAYFLPKNVDTMQLAMLAAVGGGVEVEQNFLDRKLIALTAYYGELPRDC

Target ORF information:

RefSeq Version	XM_012312366.2
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris trimethylguanosine synthase (LOC100643170), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012312366.2

ORF Insert Sequence:

ATGAGTGACC GCTTTTGGGA ACCTTTAGCC GAAGTGTACA TCGCAAAACA AGCTGAGTCT 
ACTGACCCTG ATCATTACAT TTATTGTCTT TGCAGCAGAG TATTTATTCG ACACCCAGAA 
GTTTATACTG CCCTTGCTCT AGAAGATGCT AGTAAAGATG AAGATATTGA GAGCGATTGT 
GTTGGAGAAA TGTTAGACCT CCATAAAAGG TCTATTATAG AAACACAATC CATAGAGAAA 
CATGATGAAG AACCAGTGAG CTGTTACTGC AGTGCATCAC ACACAGACAA CAATTATTCC 
ACAGATGAAC ATGACTCTGT TCGTGATTCG GCTCATCGTC CTACTGCACA TTCCCTCACG 
CAAAAATTGG GCCTGCACCA GTCAGATTCA GGTGCAGACC TATCAGAATA TCATGATCAA 
CATGAGGCTA AAAGCATACA AAATCTATTG GCATCTTATG GGAAAACTTT TCAAAATACA 
GAGACTGAAC TGGAAGATGA ATTTGACAAA GTGAATGTAA TTGCACAGCA CCAAGAAGAT 
AAAGATGTAG AATTTGAATG TAAAATAACA AACATTCACT CATCAGATCA TCTTGCAGAT 
AACATTGAAA TTTTAAGTTA TGATCCATCC ATTTGTAATG AAAAGGAACT TCACATAGAA 
AATCCATCAT GTATTTTAGA AGACAAAGAT TTTGAGATAC ATAATATATT TAAAAATGTT 
TCTTTGAATG ATACACAAAC TTATAAGGAA GATGTCCAAC TCAAGTACAA CTGTGACCTT 
AGCTCGTGTG ACTATCCATA TTATTCTATG TATTCTAAAA CTAAGAGTAT AATAGATATG 
GCATGGGAGA AATATTGGTC TAAAAATGGT GAACGTATAA TTTGGACAAC TTGGATTAGG 
AAATATGCAG ATTATATAAA TCCAGAATAT TTGCAACAAA ATGCTCATTT AATAAGTAAT 
GAAAAGTTAG AAGTGAAAGA TACTATCGAA AAATTTTCAG AGCAAAATAC ATGTTTTCCT 
AGTCAAGCGC ACAGAAATTG TGAACTTGAT CGCTCAAATT TTGAGGGAAT ATTTAGCAAA 
AGTAATAATG CTGGTAATAA TGAAATAAAA GCAAAAGACA CGTGTAGTAT TAATTTTTCA 
TTTGATGACA TAAGCAAGCA AGAGATAAGT GACAAAGATG CGGAAGATAA TAGAAAGAAA 
TTGACAAATT TTGAAGCATC ACTGGAAACT GGAGATGGTT GGAATCCATT AAGTCCTTTT 
AGTATGGAAG AAAGTTACAA CCAACAATCT AATGCGGAAG ACGAAAGACT TTTAACAAGG 
TGTGATTCTA TTAACGAGTC AATAGCAAAA ACAAATGCAA CATCAGATTC TATGACAAAC 
GTCACAAAGA TGACCCTTAC TAGTTCTAGT TGTGACTCTA TTTCTACAAA TTCATCTAGT 
CTCATCAGTT CTGTAACTAG TTCCATTGAA AGCAATATAA CAAACACAAG TTCTGATCAA 
GATAATGATC AGGAATATAC ACCAGAAGAT ACTGATAAGT ACTGGCAACA TTTATGGAAA 
GAAAATTTCC AAATAGAGTA CCAAAATCAT TATAAACTAT TTATAGAAAA ATATAAAAGG 
GAGCAACCTG AAATTAGCAA TCAAAGTTAT AGCCAGGAAT ATAATGATAT AGATATGAAA 
GAGGAAGGAA GTGATGAAAG TATAGACCAA GATTTTACAC AAAGGGAAAT AGACACATCT 
AATGAGATTA ATATTTCTGA AAGTAAAAGT GCACCTAGTA AAAATTTTTC TTATTCTAAG 
AAAGATAAGA CAAAAAAGAA ACGATTAATT ATGGAATCAG TGGGAATGCT CATTCAAAAT 
TTAACAGTAG AAGCAAAAGA TAATGAAACT GATGTACATA AAAATGAAAA AATTAATGAA 
ATGGAATGTT CACAAGAAGT TGAACATGAT CAAACTTCAA CTACAGAAAA TGAAAATGGT 
GCAATTAATT CCAATGATGC TAGTGGCAAC AATAGTATTC AACGTAAATA TTCTAATGAA 
GGAGATGGAG ATGAGCCCAA TGAAGATAAA CCTATAACAT TAAAACGGAG TCATGAGGCT 
GATTATGATG ACACGGAAGA AGGCCTGGAA TCAGTAAAAA AAGCATTTTC ATTGATGGGA 
TATACTTTCA ACGAAAATTA CGAACAATCT AATTTCCAAG GAGAAGTAGT ATATAGAAAA 
AAANATATTA GGTTACAAAA TAGACAATTA AAAATGAAAT ATAAACCCAA GCCAATAAAT 
AAACATATAT ACTTTGATGA TAACGGAGTG GAAATTACTA ACACTATAGA CAAAGTGAAG 
CATTATTTAT CATACTGTCC TGTTTTGCCA CCAGTAAAAA CAGAATTACA ATCCTCTGAA 
AAAGGTTCCT ACACAGCACA ATTAACGTCA AGTTCTGATG ATGAATGTGA TCATAGCCTT 
AAGTCAAAGT TACAAACAAA ACGATTTGTA TTTAGTAAAC CAAGCACAAG TTCAATAGAT 
TCAGGAGCAG ATAGAAAACA ATTTGATGAT GTTCACGATA CCTTAAACGT ATTTGATAAA 
CAGGGCAATG TTTTCCAAAG TATCAAAGAT GATAATATAT ACTTTGATCA GGATGAAACA 
AGCAATTCCA CAAAATGTGT ACCAGAAAAT CAAATTTTCG AACAAGAGAA CTTTGATGTA 
TTAAATGTCA ATATGGATAT AGATGAAAGC TATAGTAACA ACGAAATACA AAGTATGAAA 
TTATCAGATG AAGATAGCGT AAAGAAGACG CAAAAGAAAA AACGAAGGAA GCCACCCAAA 
AGAAATGTAA GCCTGCCACT AGAAATAGAC AATGATAAAA CTTTAATGAA ATATTGGTTC 
AAAAGATACC GCCTATTTAG CAAATTTGAT CAAGGCATTA AAATGGATCG TGAGAGCTGG 
TATTCTGTAA CACCAGAAAA AATTGCGGAG CACATAGCTC AAAGATGCAA ATGCGATACC 
GTAATCGATG CATTCTGTGG AGCAGGAGGG AACGCTATTC AATTTGCTTT TACATGTGAA 
AGAGTTTTCG CAATAGATAT AGATCCAGCT AAAATCGAAA TTGCTCGAAA TAATGCTCGA 
GTTTATGGCG TTGAAGACAG AATAGAATTC ATTGTTGGAG ATTTTATTAA ACTCGCATCA 
AAGTTAAGCG CAGATGTTGT GTTTCTAAGT CCTCCATGGG GAGGACCTGA ATATGCCAAG 
AATGAAACAT TTGATCTCAA TAATATAATG CTTCCCATTG GTGGTGTAAA TTTATTTAAT 
ATAGCAAGAA AAATCACAGA TCATGTGGCC TACTTTCTAC CTAAAAATGT AGATACAATG 
CAGCTTGCCA TGTTAGCCGC AGTAGGTGGT GGTGTAGAAG TGGAACAAAA CTTTCTTGAT 
AGAAAGTTAA TAGCTTTAAC AGCTTATTAT GGCGAACTGC CCAGAGACTG TTAG