NLK cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLK
Entrez Gene ID	104640909
Full Name	nemo like kinase
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010309425.1	XP_010307727.1	serine/threonine-protein kinase NLK

Pathways from BioSystems
Gene Ontology

The following NLK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa189889	XM_010309425.1 Latest version!	Balearica regulorum gibbericeps nemo-like kinase (NLK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLK ( XM_010309425.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010307727.1 Balearica regulorum gibbericeps nemo-like kinase (NLK), mRNA.

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CloneID	OBa189889
Clone ID Related Accession (Same CDS sequence)	XM_010309425.1
Accession Version	XM_010309425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	serine/threonine-protein kinase NLK
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010773703.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTTT GTGGCACAAC AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACG 
TCTGCGGCAG CAGCAGGTCA CCACCACCAC CATCACCACC ACCTTCCTCA CCTCCCTCCT 
CCTCACCTCC ATCACCACCA CCACCCTCAA CATCACTTGC ATCCAGGCTC TGCAGCTGCG 
GTACATCCCG TACAGCAGCA TGCCTCTTCG GCAGCTGCGG CCGCGGCAGC TGCGGCCGCG 
GCCGCGGCAA TGTTAAACCC AGGGCAGCAG CAGCCATATT TCCCATCACC GGCACCGGGT 
CAGGCCCCTG GACCAGCTGC AGCAGCCCCA GCTCAGGTAC AAGCTGCAGC AGCTGCTGCA 
GTTAAAGCGC ACCATCATCA GCACTCACAA CATCCACAAC AGCAACTGGA TATTGAGCCG 
GACAGACCTA TTGGATATGG AGCCTTTGGT GTTGTCTGGT CAGTAACAGA TCCACGAGAT 
GGAAAGAGAG TTGCACTCAA AAAGATGCCC AACGTCTTCC AAAATTTGGT CTCTTGTAAA 
AGAGTCTTCC GGGAGTTGAA GATGTTGTGT TTTTTTAAGC ATGACAATGT ACTCTCTGCC 
CTCGACATAC TCCAGCCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACTGAA 
TTGATGCAGA GTGACCTCCA TAAGATTATC GTCTCTCCTC AGCCACTCAG CTCGGATCAT 
GTCAAAGTTT TTCTTTACCA GATTTTAAGA GGTCTGAAAT ATCTACATTC TGCTGGCATT 
TTACATCGAG ACATTAAACC AGGGAACCTA CTTGTGAACA GCAACTGTGT TCTTAAGATT 
TGTGATTTTG GATTGGCCAG GGTGGAAGAA TTAGATGAAT CTCGTCACAT GACTCAGGAA 
GTTGTCACTC AGTATTATCG AGCTCCAGAA ATCCTGATGG GCAGCCGTCA CTACAGCAAT 
GCTATTGACA TCTGGTCTGT AGGCTGTATC TTTGCAGAAT TACTTGGGCG AAGAATATTG 
TTTCAGGCAC AGAGTCCCAT ACAGCAGCTG GATTTGATCA CAGACCTGTT GGGAACACCG 
TCGCTGGAAG CAATGAGGAC GGCTTGTGAA GGCGCCAAGG CACACATACT CAGGGGTCCT 
CATAAACAGC CATCCCTTCC TGTCCTATAT ACACTTTCCA GTCAAGCTAC ACATGAAGCA 
GTTCATCTCC TTTGCAGAAT GTTGGTCTTT GATCCATCCA AAAGAATATC CGCTAAGGAT 
GCCTTAGCTC ACCCATATCT CGATGAAGGG CGGTTGCGAT ACCACACTTG TATGTGTAAA 
TGTTGTTTTT CCACATCTAC TGGCAGAGTT TATACTAGTG ATTTTGAACC CATCACGAAT 
CCCAAATTTG ATGACACTTT TGAAAAGAAC CTCAGTTCTG TTCGGCAGGT TAAAGAAATA 
ATTCATCAGT TCATTTTGGA ACAGCAGAAA GGAAACAGAG TGCCTCTCTG CATCAACCCT 
CAGTCTGCTG CTTTTAAGAG CTTTATTAGT TCCACGGTTG CTCAACCATC TGAGATGCCG 
CCATCTCCAC TGGTTTGGGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010307727.1
CDS	1..1584
Translation	MSLCGTTANAKMMAAYNGGTSAAAAGHHHHHHHHLPHLPPPHLHHHHHPQHHLHPGSAAAVHPVQQHASSAAAAAAAAAAAAAMLNPGQQQPYFPSPAPGQAPGPAAAAPAQVQAAAAAAVKAHHHQHSQHPQQQLDIEPDRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHIDYFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKICDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQLDLITDLLGTPSLEAMRTACEGAKAHILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKDALAHPYLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPITNPKFDDTFEKNLSSVRQVKEIIHQFILEQQKGNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE

Target ORF information:

RefSeq Version	XM_010309425.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps nemo-like kinase (NLK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010309425.1

ORF Insert Sequence:

ATGTCTCTTT GTGGCACAAC AGCCAACGCA AAAATGATGG CGGCTTACAA TGGCGGTACG 
TCTGCGGCAG CAGCAGGTCA CCACCACCAC CATCACCACC ACCTTCCTCA CCTCCCTCCT 
CCTCACCTCC ATCACCACCA CCACCCTCAA CATCACTTGC ATCCAGGCTC TGCAGCTGCG 
GTACATCCCG TACAGCAGCA TGCCTCTTCG GCAGCTGCGG CCGCGGCAGC TGCGGCCGCG 
GCCGCGGCAA TGTTAAACCC AGGGCAGCAG CAGCCATATT TCCCATCACC GGCACCGGGT 
CAGGCCCCTG GACCAGCTGC AGCAGCCCCA GCTCAGGTAC AAGCTGCAGC AGCTGCTGCA 
GTTAAAGCGC ACCATCATCA GCACTCACAA CATCCACAAC AGCAACTGGA TATTGAGCCG 
GACAGACCTA TTGGATATGG AGCCTTTGGT GTTGTCTGGT CAGTAACAGA TCCACGAGAT 
GGAAAGAGAG TTGCACTCAA AAAGATGCCC AACGTCTTCC AAAATTTGGT CTCTTGTAAA 
AGAGTCTTCC GGGAGTTGAA GATGTTGTGT TTTTTTAAGC ATGACAATGT ACTCTCTGCC 
CTCGACATAC TCCAGCCTCC ACACATTGAC TATTTTGAAG AAATATATGT TGTCACTGAA 
TTGATGCAGA GTGACCTCCA TAAGATTATC GTCTCTCCTC AGCCACTCAG CTCGGATCAT 
GTCAAAGTTT TTCTTTACCA GATTTTAAGA GGTCTGAAAT ATCTACATTC TGCTGGCATT 
TTACATCGAG ACATTAAACC AGGGAACCTA CTTGTGAACA GCAACTGTGT TCTTAAGATT 
TGTGATTTTG GATTGGCCAG GGTGGAAGAA TTAGATGAAT CTCGTCACAT GACTCAGGAA 
GTTGTCACTC AGTATTATCG AGCTCCAGAA ATCCTGATGG GCAGCCGTCA CTACAGCAAT 
GCTATTGACA TCTGGTCTGT AGGCTGTATC TTTGCAGAAT TACTTGGGCG AAGAATATTG 
TTTCAGGCAC AGAGTCCCAT ACAGCAGCTG GATTTGATCA CAGACCTGTT GGGAACACCG 
TCGCTGGAAG CAATGAGGAC GGCTTGTGAA GGCGCCAAGG CACACATACT CAGGGGTCCT 
CATAAACAGC CATCCCTTCC TGTCCTATAT ACACTTTCCA GTCAAGCTAC ACATGAAGCA 
GTTCATCTCC TTTGCAGAAT GTTGGTCTTT GATCCATCCA AAAGAATATC CGCTAAGGAT 
GCCTTAGCTC ACCCATATCT CGATGAAGGG CGGTTGCGAT ACCACACTTG TATGTGTAAA 
TGTTGTTTTT CCACATCTAC TGGCAGAGTT TATACTAGTG ATTTTGAACC CATCACGAAT 
CCCAAATTTG ATGACACTTT TGAAAAGAAC CTCAGTTCTG TTCGGCAGGT TAAAGAAATA 
ATTCATCAGT TCATTTTGGA ACAGCAGAAA GGAAACAGAG TGCCTCTCTG CATCAACCCT 
CAGTCTGCTG CTTTTAAGAG CTTTATTAGT TCCACGGTTG CTCAACCATC TGAGATGCCG 
CCATCTCCAC TGGTTTGGGA ATAA