THBS1 cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	THBS1
Entrez Gene ID	104638462
Full Name	thrombospondin 1
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010306260.1	XP_010304562.1	thrombospondin-1

Pathways from BioSystems
Gene Ontology

The following THBS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa188425	XM_010306260.1 Latest version!	Balearica regulorum gibbericeps thrombospondin 1 (THBS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THBS1 ( XM_010306260.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010304562.1 Balearica regulorum gibbericeps thrombospondin 1 (THBS1), mRNA.

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CloneID	OBa188425
Clone ID Related Accession (Same CDS sequence)	XM_010306260.1
Accession Version	XM_010306260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	thrombospondin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010765720.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGGCCAA CGACTGTTCT CTTCCTGCTC CTCATGCTCG GTGTCTCGGA AGGCAAGCGC 
GCTGCAGAGT CCCGAAGTGA TGATAACAGT GTCTTCGATC TCTTTGAACT CATCGGCTTC 
ATTCGGAAGG GAGCTGGGCG CCGGGCACCC GGGGTGCACC TGGTGAAGGG ACCAGAATCC 
TCCAGCCCTG CTTACCGCAT TGAAGATGCC AGTCGCATCC CTGCTGTCCC TGACTCCAAG 
TTCCAAGACT TATTAGATGC CATCCATGCT GAGAAGGGCT TCATTCTCCT TGCAACCCTC 
CGGCAAGCCA AGAAGAGCAG AGGCACGCTG CTGTCCGTGG AACAGAAAGA CGGCTCTGGT 
CATGTCTTCA GTCTAGTATC AAATGGCAAG GCAGGCACCC TGGACCTGAG CCTTTCTGGT 
GATGGCAAGC AGCAGTTAGT GTCAGTAGAG GACGCCTTGC TAGCTACAGG ACATTGGAAG 
AATATCACCC TGTTTGTGCA GGAGGACCGG GCTCAGCTCT ATGTGGGGTG TGAGAAAATG 
GAGAATGCTG AACTAGACAT CCCCATCCAG AACATCTTCA CTAGGGACCT GGCCAGCAGT 
GCCAGGCTCC GTATTGCCAA AGGGGGAGTC AATGACAACT TCCAGGGACT GCTGCAGAAT 
GTGCGGTTTG TGTTTGGAAC AACTCTGGAA ACTATCCTGA GGAACAAAGG CTGCTCCAGC 
TCCACTAGTG CAATCATTAC TTTGGACAAC CCCATCAATG GTTCTAGCCC AGCTATCCGC 
ACTAATTACA TTGGCCATAA AACGAAGGAC ATCCAAGCAG TCTGTGGTTT TTCCTGTGAT 
GAACTAACAA ATATGTTTGT GGAACTGCAA GGGCTCCGGT CCATGGTTAC AACACTTCAA 
GACAGAGTTC GCAAAGTGAC TGAAGAAAAT GAACTGATTG CCAAAGTGGT CCAGATCACT 
CCTGGAGTAT GTATTCATAA TGGCATCTTG CACAAAAATA AAGAGGAGTG GACTATTGAC 
AGCTGCACTG AATGTACCTG CCAGAACTCT GCCACTATAT GCCGGAAAGT GTCTTGTCCT 
CTGATGCCTT GTTCCAATGC CACTGTGCCT GATGGGGAGT GCTGCCCCCG ATGTTGGCCT 
AGTGACTATG CAGATGATGG ATGGTCTCCT TGGTCTGAAT GGACTTCATG CTCTGTGACC 
TGTGGGAATG GGATTCAGCA GAGAGGGCGA TCCTGTGACA GTCTCAATAA CCGTTGTGAG 
GGGTCGTCTG TACAGACTAG GACTTGCCAC CTTCAGGAGT GCGATAAGAG ATTTAAACAG 
GATGGTGGCT GGAGTCACTG GTCACCATGG TCATCGTGTT CTGTCACTTG TGGCACGGGC 
ATCATCACAA GAATTCGTCT CTGCAACTCT CCAGTACCGC AGCTGAATGG CAAACCTTGT 
GAGGGTGAGG CAAGAGAAAA CAAACCCTGC CAGAAAGATC CTTGCCCAAT TAATGGCAAC 
TGGGGACCAT GGTCTCCATG GGATGCTTGC ACAGTGACAT GTGGAGGAGG ACTTCAAAAA 
CGTAGCCGTC TCTGCAATAA TCCTGAGCCT CAGTATGGTG GGAAGACTTG CGTAGGTGAA 
GCTAGAGGGA CTCAAGTCTG CAACAAACAG GACTGTCCAA TTGATGGGTG TCTGTCCAAT 
CCCTGCTTTG CGGGGACTAC GTGTACCAGC TCCCCAGATG GTTCCTGGAA GTGTGGTGCC 
TGTCCTGCTG GCTACCATGG TGATGGTGTC CACTGCCAAG ATATTGATGA GTGCAAAGAG 
GTTCCTGATG CCTGCTTTGT CTTCAACGGG GTGCACAGGT GTGAGAATAC TGAACCTGGG 
TACAACTGTC TGCCTTGTCC ACCACGTTTC ACTGGCACAC AGCCATTTGG TCGCAGTGTT 
GAAGATGCCA TGGCTAACAA GCAGGTCTGC AAGCCACGTA ATCCATGCAC AGATGGAACA 
CATGACTGCA ACAAGAATGC CAAATGCAAT TACCTTGGCC ACTTCAGTGA CCCTATGTAT 
CGTTGTGAAT GTAAACCAGG CTATGCTGGC AATGGCATAA TCTGTGGAGA AGATACTGAC 
TTGGATGGGT GGCCAAATGA GAACCTTGTT TGTGTTGCCA ATGCCACTTA CCACTGTAAA 
AAAGATAACT GCCCTAATCT GCCTAACTCT GGGCAGGAAG ACTACGATAA GGATGGTATT 
GGTGATGCCT GTGACAACGA TGACGATGAT GATGGTATTC CTGATGACCG GGACAACTGT 
CCGTTCATCT ACAACCCACA GCAGTATGAC TATGACAGGG ATGATGTTGG TGACCGCTGT 
GATAACTGCC CCTACAATCA CAACCCTGAT CAAACTGACA CTGACAACAA TGGAGAAGGA 
GACGCATGTG CAGTAGATAT TGATGGAGAT GGGGTCCTTA ACGAAAGGGA CAACTGCCAA 
TATGTCTACA ATGTAGACCA GAGGGATACA GACTTGGATG GTGTTGGAGA TCAGTGTGAT 
AACTGTCCAC TGGAACACAA TCCTGACCAG GAAGACACTG ATTCTGACCG CATAGGTGAT 
CAGTGTGACA ACAACCAGGA CATAGATGAA GATGGCCATC AAAATAATCT TGATAACTGC 
CCCTATGTGC CCAATGCCAA TCAAGCTGAC CATGACAAGG ATGGAAAAGG TGATGCCTGT 
GACCATGATG ATGACAACGA TGGCATGCCT GATGACAAAG ATAACTGCAG ATTGGTTGCC 
AACCCAGATC AAGCTGATTC TGATGGTGAT GGACGTGGAG ATGCTTGCAA AGATGACTTT 
GACCAAGACA GTGTGCCAGA CATTGATGAT ATCTGCCCTG AAAATGTGGA CATTAGCGAG 
ACTGATTTCC GAAGATTTCA GATGATTCCC CTGGATCCCA AAGGAACATC CCAAAATGAT 
CCAAACTGGG TTGTTCGTCA CCAGGGTAAA GAACTGGTTC AGACAGTCAA CTGTGACCCT 
GGCCTTGCAG TTGGTTTTGA TGAATTCAAT GCTGTGGACT TCAGTGGCAC CTTCTTCATC 
AATACAGAAA GGGATGATGA TTATGCTGGC TTTGTATTTG GCTACCAATC TAGCAGTCGT 
TTCTATGTTG TCATGTGGAA GCAGATCACC CAGTCTTATT GGGACTCCAC ACCAACCAAA 
GCTCAAGGCT ACTCAGGTCT CTCCATCAAA GTTGTGAATT CCACCACTGG CCCAGGAGAA 
CACCTTCGTA ATGCTCTGTG GCACACAGGA AACACTCCTG GCCAGGTGAG AACTTTGTGG 
CATGACCCTC GTCACATAGG CTGGAAAGAC TTCACTGCAT ACAGATGGCG TCTTAGCCAT 
AGGCCAAAGA CTGGTTACAT CAGGGTTGTA ATGTATGAAG GGAAGAAAAT CATGGCAGAC 
TCAGGACCAA TCTATGATAA AACCTATGCT GGTGGTCGGC TAGGATTATT TGTCTTCTCT 
CAAGAAATGG TGTTCTTTTC AGATCTTAAA TATGAATGCA GAGATCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010304562.1
CDS	1..3531
Translation	MGPTTVLFLLLMLGVSEGKRAAESRSDDNSVFDLFELIGFIRKGAGRRAPGVHLVKGPESSSPAYRIEDASRIPAVPDSKFQDLLDAIHAEKGFILLATLRQAKKSRGTLLSVEQKDGSGHVFSLVSNGKAGTLDLSLSGDGKQQLVSVEDALLATGHWKNITLFVQEDRAQLYVGCEKMENAELDIPIQNIFTRDLASSARLRIAKGGVNDNFQGLLQNVRFVFGTTLETILRNKGCSSSTSAIITLDNPINGSSPAIRTNYIGHKTKDIQAVCGFSCDELTNMFVELQGLRSMVTTLQDRVRKVTEENELIAKVVQITPGVCIHNGILHKNKEEWTIDSCTECTCQNSATICRKVSCPLMPCSNATVPDGECCPRCWPSDYADDGWSPWSEWTSCSVTCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHLQECDKRFKQDGGWSHWSPWSSCSVTCGTGIITRIRLCNSPVPQLNGKPCEGEARENKPCQKDPCPINGNWGPWSPWDACTVTCGGGLQKRSRLCNNPEPQYGGKTCVGEARGTQVCNKQDCPIDGCLSNPCFAGTTCTSSPDGSWKCGACPAGYHGDGVHCQDIDECKEVPDACFVFNGVHRCENTEPGYNCLPCPPRFTGTQPFGRSVEDAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHFSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDNDDDDDGIPDDRDNCPFIYNPQQYDYDRDDVGDRCDNCPYNHNPDQTDTDNNGEGDACAVDIDGDGVLNERDNCQYVYNVDQRDTDLDGVGDQCDNCPLEHNPDQEDTDSDRIGDQCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGMPDDKDNCRLVANPDQADSDGDGRGDACKDDFDQDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGFDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQSYWDSTPTKAQGYSGLSIKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGYIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

Target ORF information:

RefSeq Version	XM_010306260.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps thrombospondin 1 (THBS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010306260.1

ORF Insert Sequence:

ATGGGGCCAA CGACTGTTCT CTTCCTGCTC CTCATGCTCG GTGTCTCGGA AGGCAAGCGC 
GCTGCAGAGT CCCGAAGTGA TGATAACAGT GTCTTCGATC TCTTTGAACT CATCGGCTTC 
ATTCGGAAGG GAGCTGGGCG CCGGGCACCC GGGGTGCACC TGGTGAAGGG ACCAGAATCC 
TCCAGCCCTG CTTACCGCAT TGAAGATGCC AGTCGCATCC CTGCTGTCCC TGACTCCAAG 
TTCCAAGACT TATTAGATGC CATCCATGCT GAGAAGGGCT TCATTCTCCT TGCAACCCTC 
CGGCAAGCCA AGAAGAGCAG AGGCACGCTG CTGTCCGTGG AACAGAAAGA CGGCTCTGGT 
CATGTCTTCA GTCTAGTATC AAATGGCAAG GCAGGCACCC TGGACCTGAG CCTTTCTGGT 
GATGGCAAGC AGCAGTTAGT GTCAGTAGAG GACGCCTTGC TAGCTACAGG ACATTGGAAG 
AATATCACCC TGTTTGTGCA GGAGGACCGG GCTCAGCTCT ATGTGGGGTG TGAGAAAATG 
GAGAATGCTG AACTAGACAT CCCCATCCAG AACATCTTCA CTAGGGACCT GGCCAGCAGT 
GCCAGGCTCC GTATTGCCAA AGGGGGAGTC AATGACAACT TCCAGGGACT GCTGCAGAAT 
GTGCGGTTTG TGTTTGGAAC AACTCTGGAA ACTATCCTGA GGAACAAAGG CTGCTCCAGC 
TCCACTAGTG CAATCATTAC TTTGGACAAC CCCATCAATG GTTCTAGCCC AGCTATCCGC 
ACTAATTACA TTGGCCATAA AACGAAGGAC ATCCAAGCAG TCTGTGGTTT TTCCTGTGAT 
GAACTAACAA ATATGTTTGT GGAACTGCAA GGGCTCCGGT CCATGGTTAC AACACTTCAA 
GACAGAGTTC GCAAAGTGAC TGAAGAAAAT GAACTGATTG CCAAAGTGGT CCAGATCACT 
CCTGGAGTAT GTATTCATAA TGGCATCTTG CACAAAAATA AAGAGGAGTG GACTATTGAC 
AGCTGCACTG AATGTACCTG CCAGAACTCT GCCACTATAT GCCGGAAAGT GTCTTGTCCT 
CTGATGCCTT GTTCCAATGC CACTGTGCCT GATGGGGAGT GCTGCCCCCG ATGTTGGCCT 
AGTGACTATG CAGATGATGG ATGGTCTCCT TGGTCTGAAT GGACTTCATG CTCTGTGACC 
TGTGGGAATG GGATTCAGCA GAGAGGGCGA TCCTGTGACA GTCTCAATAA CCGTTGTGAG 
GGGTCGTCTG TACAGACTAG GACTTGCCAC CTTCAGGAGT GCGATAAGAG ATTTAAACAG 
GATGGTGGCT GGAGTCACTG GTCACCATGG TCATCGTGTT CTGTCACTTG TGGCACGGGC 
ATCATCACAA GAATTCGTCT CTGCAACTCT CCAGTACCGC AGCTGAATGG CAAACCTTGT 
GAGGGTGAGG CAAGAGAAAA CAAACCCTGC CAGAAAGATC CTTGCCCAAT TAATGGCAAC 
TGGGGACCAT GGTCTCCATG GGATGCTTGC ACAGTGACAT GTGGAGGAGG ACTTCAAAAA 
CGTAGCCGTC TCTGCAATAA TCCTGAGCCT CAGTATGGTG GGAAGACTTG CGTAGGTGAA 
GCTAGAGGGA CTCAAGTCTG CAACAAACAG GACTGTCCAA TTGATGGGTG TCTGTCCAAT 
CCCTGCTTTG CGGGGACTAC GTGTACCAGC TCCCCAGATG GTTCCTGGAA GTGTGGTGCC 
TGTCCTGCTG GCTACCATGG TGATGGTGTC CACTGCCAAG ATATTGATGA GTGCAAAGAG 
GTTCCTGATG CCTGCTTTGT CTTCAACGGG GTGCACAGGT GTGAGAATAC TGAACCTGGG 
TACAACTGTC TGCCTTGTCC ACCACGTTTC ACTGGCACAC AGCCATTTGG TCGCAGTGTT 
GAAGATGCCA TGGCTAACAA GCAGGTCTGC AAGCCACGTA ATCCATGCAC AGATGGAACA 
CATGACTGCA ACAAGAATGC CAAATGCAAT TACCTTGGCC ACTTCAGTGA CCCTATGTAT 
CGTTGTGAAT GTAAACCAGG CTATGCTGGC AATGGCATAA TCTGTGGAGA AGATACTGAC 
TTGGATGGGT GGCCAAATGA GAACCTTGTT TGTGTTGCCA ATGCCACTTA CCACTGTAAA 
AAAGATAACT GCCCTAATCT GCCTAACTCT GGGCAGGAAG ACTACGATAA GGATGGTATT 
GGTGATGCCT GTGACAACGA TGACGATGAT GATGGTATTC CTGATGACCG GGACAACTGT 
CCGTTCATCT ACAACCCACA GCAGTATGAC TATGACAGGG ATGATGTTGG TGACCGCTGT 
GATAACTGCC CCTACAATCA CAACCCTGAT CAAACTGACA CTGACAACAA TGGAGAAGGA 
GACGCATGTG CAGTAGATAT TGATGGAGAT GGGGTCCTTA ACGAAAGGGA CAACTGCCAA 
TATGTCTACA ATGTAGACCA GAGGGATACA GACTTGGATG GTGTTGGAGA TCAGTGTGAT 
AACTGTCCAC TGGAACACAA TCCTGACCAG GAAGACACTG ATTCTGACCG CATAGGTGAT 
CAGTGTGACA ACAACCAGGA CATAGATGAA GATGGCCATC AAAATAATCT TGATAACTGC 
CCCTATGTGC CCAATGCCAA TCAAGCTGAC CATGACAAGG ATGGAAAAGG TGATGCCTGT 
GACCATGATG ATGACAACGA TGGCATGCCT GATGACAAAG ATAACTGCAG ATTGGTTGCC 
AACCCAGATC AAGCTGATTC TGATGGTGAT GGACGTGGAG ATGCTTGCAA AGATGACTTT 
GACCAAGACA GTGTGCCAGA CATTGATGAT ATCTGCCCTG AAAATGTGGA CATTAGCGAG 
ACTGATTTCC GAAGATTTCA GATGATTCCC CTGGATCCCA AAGGAACATC CCAAAATGAT 
CCAAACTGGG TTGTTCGTCA CCAGGGTAAA GAACTGGTTC AGACAGTCAA CTGTGACCCT 
GGCCTTGCAG TTGGTTTTGA TGAATTCAAT GCTGTGGACT TCAGTGGCAC CTTCTTCATC 
AATACAGAAA GGGATGATGA TTATGCTGGC TTTGTATTTG GCTACCAATC TAGCAGTCGT 
TTCTATGTTG TCATGTGGAA GCAGATCACC CAGTCTTATT GGGACTCCAC ACCAACCAAA 
GCTCAAGGCT ACTCAGGTCT CTCCATCAAA GTTGTGAATT CCACCACTGG CCCAGGAGAA 
CACCTTCGTA ATGCTCTGTG GCACACAGGA AACACTCCTG GCCAGGTGAG AACTTTGTGG 
CATGACCCTC GTCACATAGG CTGGAAAGAC TTCACTGCAT ACAGATGGCG TCTTAGCCAT 
AGGCCAAAGA CTGGTTACAT CAGGGTTGTA ATGTATGAAG GGAAGAAAAT CATGGCAGAC 
TCAGGACCAA TCTATGATAA AACCTATGCT GGTGGTCGGC TAGGATTATT TGTCTTCTCT 
CAAGAAATGG TGTTCTTTTC AGATCTTAAA TATGAATGCA GAGATCCATA A