SPAM1 cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAM1
Entrez Gene ID	104633729
Full Name	sperm adhesion molecule 1
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010300031.1	XP_010298333.1	hyaluronidase PH-20

Pathways from BioSystems
Gene Ontology

The following SPAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa182849	XM_010300031.1 Latest version!	Balearica regulorum gibbericeps sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAM1 ( XM_010300031.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010298333.1 Balearica regulorum gibbericeps sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), mRNA.

Price & Availability↑

CloneID	OBa182849
Clone ID Related Accession (Same CDS sequence)	XM_010300031.1
Accession Version	XM_010300031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	hyaluronidase PH-20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010734307.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACTC TAAGACAAGG TTTTGGTACC TGTGTTACCT GTACGTATCC TATAGCATCC 
GGTATGGTGT TCACCACTCT TCTAGTTTCT TGCTGCTCAT CTCTGAACAT AAGAGCTCGT 
CCACTTGTTT CTAATTCACC TTTCCTTTCT ATCTGGAATG CTCCTACAGA ACTTTGTACT 
GAAAGGATTG GAGTGCAGCT GGACATGAAA TTTTTTTCTC TCATTGGAAG CACACTGAAG 
ACATCCATTG GGCAAAACAT CACTCTCTTC TATCCAGACA GGCTTGGCTA CTATCCTTAC 
AAAAATGAAG TCACAGGAGA GGCGTTCAAT GGAGGACTCC CCCAGCTCTC GCTGCTGCAG 
AATCATTTAA AAAAAGCCAA AGAAGACATC CAGTTCTATA TTCCTTCAGA TGAACAGTTT 
GGATTGGCTG TCATTGACTG GGAAAATTGG AGACCTGTGT GGATAAGGAA CTGGGGATCA 
AAAGACATTT ATAGGCAGGA ATCCATTGAA CTGGTTCAGC ANNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNAGTCTATC AGAAGCCGAA GCCAGGACTA TAGCTAAAAT GGAATTTGAA 
GCTGCAGCAA AATCAATTAT GTTGGAATCT TTAAAGCTGG GCATAGAAAT GAAGCCAAAT 
CGTCTGTGGG GATATTACCT TTATCCAGAC TGTTATAACT ATGATTACAA ACAAAACCCA 
CATAATTATA CAGGAACCTG TTTAGATATT GAAATAGAAA GAAATAATGA GCTTAATTGG 
TTGTGGGAGG AAAGTACAGC ACTTTATCCA TCTATCTATC TAGAGACAGC CTTAAGATCT 
TCCAGAAATG CCCAGCTCTT TGTTCGCAAC AGAGTTCAAG AAGCCATTAG AATTTCGTAT 
GTTTCTAATT CTACCCATCC CCTTCCAGTT TTTGTATATA CACGTCCAGT ATTCACAGAT 
GTCTATGAAG AATATCTCTC CCAGGATGAC CTGGTAAATA CCATCGGAGA ATCTGCTGCA 
TTGGGTGCTT CTGGAATTGT GATCTGGGGT GATATGAATT TAACACAAAA TAAGAACACC 
TGTAGAACTC TGGACAACTA CCTTAGGAGG ACTTTGACCC CGTACCTCAT CAATGTCACA 
ATGGCAGCCA GAATCTGTAG CCAAGTGTTA TGCCAAGACT CTGGTGCTTG TGCACGGAAA 
AAATGGAATT CCAGTGACTA TCTTCACTTG AACCCTGAGA ATATCGTCAT TCAAATGACA 
AAGGATGGAA AATACACTCT GCAAGGGCAA CCAGCATTTC AGGACCTGCA AACACTCATA 
GAAAAATTTG ATTGCCACTG TTACGCAGGG CACAGTTGTG AACCTAGAAC TGATATAAAT 
GACATCCATT ACCTCCGTGC TTGCATTTCA GAAGATATTT GCATTCAGCT ATCCTCAAAT 
TCACTTTCTA ATATAGAAGC CTCTGAAGAA AAGATCCTGT CTAACAGAAC TGTATTTTCA 
CTTACCTCTC AGAATAAGGT GACATTATCT ACACATCCTG AAATAGATTT CCAATCTACC 
TTTGGAAATA ATATACTCAA TACAACAACT GCAGAATATA GCACTGTCAC AGCTGCCACT 
AGATATGATT TAGAAGCAAA TGACACTCGT ACTTTCAGTA GTTCTTCATC CTATAAGATA 
AGGATGTTTA ATCTGTTTTG TTTAATTCTA ATTTTGAGAA CCTTAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010298333.1
CDS	1..1731
Translation	METLRQGFGTCVTCTYPIASGMVFTTLLVSCCSSLNIRARPLVSNSPFLSIWNAPTELCTERIGVQLDMKFFSLIGSTLKTSIGQNITLFYPDRLGYYPYKNEVTGEAFNGGLPQLSLLQNHLKKAKEDIQFYIPSDEQFGLAVIDWENWRPVWIRNWGSKDIYRQESIELVQnullnullnullnullnullnullnullnullnullnullnullSLSEAEARTIAKMEFEAAAKSIMLESLKLGIEMKPNRLWGYYLYPDCYNYDYKQNPHNYTGTCLDIEIERNNELNWLWEESTALYPSIYLETALRSSRNAQLFVRNRVQEAIRISYVSNSTHPLPVFVYTRPVFTDVYEEYLSQDDLVNTIGESAALGASGIVIWGDMNLTQNKNTCRTLDNYLRRTLTPYLINVTMAARICSQVLCQDSGACARKKWNSSDYLHLNPENIVIQMTKDGKYTLQGQPAFQDLQTLIEKFDCHCYAGHSCEPRTDINDIHYLRACISEDICIQLSSNSLSNIEASEEKILSNRTVFSLTSQNKVTLSTHPEIDFQSTFGNNILNTTTAEYSTVTAATRYDLEANDTRTFSSSSSYKIRMFNLFCLILILRTLI

Target ORF information:

RefSeq Version	XM_010300031.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010300031.1

ORF Insert Sequence:

ATGGAAACTC TAAGACAAGG TTTTGGTACC TGTGTTACCT GTACGTATCC TATAGCATCC 
GGTATGGTGT TCACCACTCT TCTAGTTTCT TGCTGCTCAT CTCTGAACAT AAGAGCTCGT 
CCACTTGTTT CTAATTCACC TTTCCTTTCT ATCTGGAATG CTCCTACAGA ACTTTGTACT 
GAAAGGATTG GAGTGCAGCT GGACATGAAA TTTTTTTCTC TCATTGGAAG CACACTGAAG 
ACATCCATTG GGCAAAACAT CACTCTCTTC TATCCAGACA GGCTTGGCTA CTATCCTTAC 
AAAAATGAAG TCACAGGAGA GGCGTTCAAT GGAGGACTCC CCCAGCTCTC GCTGCTGCAG 
AATCATTTAA AAAAAGCCAA AGAAGACATC CAGTTCTATA TTCCTTCAGA TGAACAGTTT 
GGATTGGCTG TCATTGACTG GGAAAATTGG AGACCTGTGT GGATAAGGAA CTGGGGATCA 
AAAGACATTT ATAGGCAGGA ATCCATTGAA CTGGTTCAGC ANNNNNNNNN NNNNNNNNNN 
NNNNNNNNNN NNAGTCTATC AGAAGCCGAA GCCAGGACTA TAGCTAAAAT GGAATTTGAA 
GCTGCAGCAA AATCAATTAT GTTGGAATCT TTAAAGCTGG GCATAGAAAT GAAGCCAAAT 
CGTCTGTGGG GATATTACCT TTATCCAGAC TGTTATAACT ATGATTACAA ACAAAACCCA 
CATAATTATA CAGGAACCTG TTTAGATATT GAAATAGAAA GAAATAATGA GCTTAATTGG 
TTGTGGGAGG AAAGTACAGC ACTTTATCCA TCTATCTATC TAGAGACAGC CTTAAGATCT 
TCCAGAAATG CCCAGCTCTT TGTTCGCAAC AGAGTTCAAG AAGCCATTAG AATTTCGTAT 
GTTTCTAATT CTACCCATCC CCTTCCAGTT TTTGTATATA CACGTCCAGT ATTCACAGAT 
GTCTATGAAG AATATCTCTC CCAGGATGAC CTGGTAAATA CCATCGGAGA ATCTGCTGCA 
TTGGGTGCTT CTGGAATTGT GATCTGGGGT GATATGAATT TAACACAAAA TAAGAACACC 
TGTAGAACTC TGGACAACTA CCTTAGGAGG ACTTTGACCC CGTACCTCAT CAATGTCACA 
ATGGCAGCCA GAATCTGTAG CCAAGTGTTA TGCCAAGACT CTGGTGCTTG TGCACGGAAA 
AAATGGAATT CCAGTGACTA TCTTCACTTG AACCCTGAGA ATATCGTCAT TCAAATGACA 
AAGGATGGAA AATACACTCT GCAAGGGCAA CCAGCATTTC AGGACCTGCA AACACTCATA 
GAAAAATTTG ATTGCCACTG TTACGCAGGG CACAGTTGTG AACCTAGAAC TGATATAAAT 
GACATCCATT ACCTCCGTGC TTGCATTTCA GAAGATATTT GCATTCAGCT ATCCTCAAAT 
TCACTTTCTA ATATAGAAGC CTCTGAAGAA AAGATCCTGT CTAACAGAAC TGTATTTTCA 
CTTACCTCTC AGAATAAGGT GACATTATCT ACACATCCTG AAATAGATTT CCAATCTACC 
TTTGGAAATA ATATACTCAA TACAACAACT GCAGAATATA GCACTGTCAC AGCTGCCACT 
AGATATGATT TAGAAGCAAA TGACACTCGT ACTTTCAGTA GTTCTTCATC CTATAAGATA 
AGGATGTTTA ATCTGTTTTG TTTAATTCTA ATTTTGAGAA CCTTAATATA A