FRMD4B cDNA ORF clone, Balaenoptera acutorostrata scammoni

Gene
Clones
gRNAs

Summary

Gene Symbol	FRMD4B
Entrez Gene ID	103020853
Full Name	FERM domain containing 4B
Gene Type	protein-coding
Organism	Balaenoptera acutorostrata scammoni

mRNA and Protein(s)

mRNA	Protein	Name
XM_007192432.1	XP_007192494.1	FERM domain-containing protein 4B isoform X2
XM_007192431.1	XP_007192493.1	FERM domain-containing protein 4B isoform X1

Pathways from BioSystems
Gene Ontology

The following FRMD4B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FRMD4B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa170516	XM_007192432.1 Latest version!	Balaenoptera acutorostrata scammoni FERM domain containing 4B (FRMD4B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa170515	XM_007192431.1 Latest version!	Balaenoptera acutorostrata scammoni FERM domain containing 4B (FRMD4B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FRMD4B ( XM_007192432.1 ) cDNA ORF clone, Balaenoptera acutorostrata scammoni -> XP_007192494.1 Balaenoptera acutorostrata scammoni FERM domain containing 4B (FRMD4B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa170516
Clone ID Related Accession (Same CDS sequence)	XM_007192432.1
Accession Version	XM_007192432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-11
Organism	Balaenoptera acutorostrata scammoni
Product	FERM domain-containing protein 4B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006731900.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Balaenoptera acutorostrata scammoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCGAG ACCATGGTTC GGAGATGACA GAAGGCAGGC ACTGCCAGGT GCACCTCCTG 
GATGACAGGA GATTGGAGCT GCTGGTTCAG CCCAAACTTT TATCAAGAGA GTTGCTGGAC 
CTCGTGGCTT CGCATTTCAA CCTGAAAGAA AAGGAGTATT TTGGAATCAC GTTTACAGAT 
GACACAGGTC AGCAGAACTG GCTACAGTTA GACCACCGAG TTCTTGACCA CGATTTGCCC 
AAGAAACCAG GCCCAACCGT TTTGCACTTT TCTGTGAGGT TTTACATTGA GAGCATATCG 
TTCCTGAAGG ATAAAACCAC CGTGGAGCTG TTTTTCCTGA ATGCAAAGGC CTGTGTGCAC 
AAGGGGCAAA TTGAAGTAGA CAGCGAAACT ATCTTCAGGT TAGCGGCGCT TATTTTACAG 
GAAGCCAAAG GGGATTACAC CAGTGATGAA AATGCCAGGA AAGATTTAAA GACATTACCA 
GCCTTTCCAA CCAAAACTCT GCAGGAGCAC CCCTCCCTTG CCTACTGTGA GGACAGGGTA 
ATTGAGCATT ATTTGAAAAT CAAAGGTCTG ACTCGGGGTC AAGCTGTGGT TCAGTATATG 
AAAATAGTAG AAGCTCTACC GACCTATGGA GTCCATTATT ATGCAGTAAA GGATAAACAA 
GGACTTCCTT GGTGGCTTGG AATCAGCTAT AAAGGAATTG GCCAGTATGA TTTGCAAGAT 
AAGGTGAAAC CTCGGAAATT ATTCCAGTGG AAACAGCTGG AGAACTTATA TTTCCGTGAG 
AAAAAGTTTG CTGTGGAAGT TCACGATCCA CGGAGGATTT CGGTATCCAG AAGAACCTTT 
GGGCAAAGTG GCCTCTTTGT GCAAACGTGG TATGCGAACT CTTCTCTCAT CAAGTCCATC 
TGGGTAATGG CAATTAGTCA GCATCAGTTT TACTTGGACC GGAAGCAAAG CAAAGCAAAA 
ATTCCTTCAG CCAGGAGTTT AGACGATATT GCAATGGATT TGACTGAGAC AGGAACACCA 
AGAGTCTCCA AAATGGTGAC ACTAGAGGCA AAAAGTCAGT TCATCATGGC AAGCAACGGC 
AGTTTAATCT CCTCAGGTTC TCAAGACTCG GAAGTCAGTG AGGAGCAAAA GAGAGAGAAA 
ATCCTTGAAC TCAAGAAGAA GGAAAAGCTG TTGCAAGAAA AACTTCTGAA AAAAGTTGAA 
GAACTTAAGA AGATCTGTTT GCGGGAGGCT GAGCTCACAG GCAAAATGCC AAAGGAGTAT 
CCACTGAACG TAGGTGAGAA AGCTCCTCAC GTCAGAAGGC GTGTAGGTAC CGCATTCAAA 
TTAGATGACA ACTTGCTGCC GAGTGAAGAG GACCCTGCTT TGCAAGAACT GGAGAGCAAT 
TTCCTAATAC AGCAAAAGCT GGTTGAAGCT GCAAAGAAAC TTGCCAACGA GCCAGATCTT 
TGTAAAACTG TGAAGAAAAA ACGAAAGCAA GATTACACGG ATGCAGTGAA AAAGCTTCAG 
GAGATTGAAA ATGCAATAAA TGAATACCGA ATTAGATGTG GAAAGAAACC CAGCCAGAAA 
GCAACAGTTA TCGTACCAGA AGACATAATC CCATCAGAAA GTAGCTCCTT GTCTGACACT 
ACCACCTATG ATGACCCCAG TGACACCTGT ACTCTTGCTG GGCAGCAATC AAGTTCAGTA 
CCTCACTCTC CAAGAATTCT TCCTCCCAAG TCTCTCGGGA TTGAGAGAAT CCATTTCAGA 
AAGTCGTCCA TCAATGAACA GTTTGTGGAT ACCAGACAGT GCAGAGAAAT GCTGTCGACA 
CACAGCAGTC CTTACAAAAC TCTGGAGAGG CGGCCCCAGG GAGGGCGAAG CGTGCCCACC 
ACACCTGTTC TCACCCGGAA CGCCTACAGC AGCAGCCACT TGCAACCCGA ATCTTCATCT 
CAGCACTGCC GCCAGCGGAG TGGAAGCCTT GAGTCCCAGT CTCATCTGCT CTCTGAGATG 
GACCACGATA AGCCATTTCC CTCCCTCTCC AAATCCCAAA GAAGCAGCAG CACAGAAATC 
CTGGATGACG GGTCTTCCTA TACCAGCCAG TCCAGCACAG AGTATTACTG CGTGACACCC 
GTGGCCGGCC CCTGTTACAC CACCCAAACT CTGGACACTC GCTCCAGGGG TCGGAGAAGG 
TCCAAGAAAC AGAACGTTTC GACTTCAAAT TCAGGAAGCA TGCCCAACCT AGCACAAAAG 
GATAGTGTGA GGAATGGCGT CTACTCAAAG AGTCAGGAGC AGCCTTCTTC CAATTACTAC 
ACTGCCGGGT ACACGCCACA TGCAGAGTGT GATCTCTATT ACAGCGGTGG CTACGTGTAC 
GAGAACGACA CTGAAGGACA GTACAGCGTC AATCCTTCCT ACCGGTCCTC CGCCCACTAT 
GGATATGACC ACCCAAGGGA CTACAGCAGG TCGTGTCATG AAGACGAGGT CGAGCGGGTG 
CCCCATAACC CGTATGCAAC TCTCCGGCTG TCGAGGAAGG CTGCTGCCAA GTCAGAGCAC 
ATCACCAAGA ACATCCACAA GGCCTTAGTG GCCGAGCACC TGCGTGGCTG GTACCAGCGG 
GCCTCTGGGC AAAAGGAGCA GGGCCACAGC ACACAGACTA GCTTTGATTC GGACAGGGGA 
TCGCAGAGAA GCCTGGGCTT TGCCGGGCTG CAGGTACCCT GTTCTCCAAG CAGCCGAGCG 
TCTTCCTACT CTTCAGTGTC TTCTACAAAT GCTTCCGGGA ACTGGAGGAC CCAGATAACT 
ATAGGGCTAT CTGAATACGA GACCCCGGCA CATTCTTCCT ACACCAGCTG CTATGGCAAC 
ATCTATAATC CTTTGCCCTC TCCAGGCAGA CCATACACAG AGACCGGTCA ACTGGACAAT 
ACAGATGGAA ACCGGCTGGA AGACAGCCTG GAGAATAGTG AGCAGAGGCT CTTTTGGCAT 
GAAGACTCAA AGCCTGGAAC ATTAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007192494.1
CDS	55..3024
Translation	MSRDHGSEMTEGRHCQVHLLDDRRLELLVQPKLLSRELLDLVASHFNLKEKEYFGITFTDDTGQQNWLQLDHRVLDHDLPKKPGPTVLHFSVRFYIESISFLKDKTTVELFFLNAKACVHKGQIEVDSETIFRLAALILQEAKGDYTSDENARKDLKTLPAFPTKTLQEHPSLAYCEDRVIEHYLKIKGLTRGQAVVQYMKIVEALPTYGVHYYAVKDKQGLPWWLGISYKGIGQYDLQDKVKPRKLFQWKQLENLYFREKKFAVEVHDPRRISVSRRTFGQSGLFVQTWYANSSLIKSIWVMAISQHQFYLDRKQSKAKIPSARSLDDIAMDLTETGTPRVSKMVTLEAKSQFIMASNGSLISSGSQDSEVSEEQKREKILELKKKEKLLQEKLLKKVEELKKICLREAELTGKMPKEYPLNVGEKAPHVRRRVGTAFKLDDNLLPSEEDPALQELESNFLIQQKLVEAAKKLANEPDLCKTVKKKRKQDYTDAVKKLQEIENAINEYRIRCGKKPSQKATVIVPEDIIPSESSSLSDTTTYDDPSDTCTLAGQQSSSVPHSPRILPPKSLGIERIHFRKSSINEQFVDTRQCREMLSTHSSPYKTLERRPQGGRSVPTTPVLTRNAYSSSHLQPESSSQHCRQRSGSLESQSHLLSEMDHDKPFPSLSKSQRSSSTEILDDGSSYTSQSSTEYYCVTPVAGPCYTTQTLDTRSRGRRRSKKQNVSTSNSGSMPNLAQKDSVRNGVYSKSQEQPSSNYYTAGYTPHAECDLYYSGGYVYENDTEGQYSVNPSYRSSAHYGYDHPRDYSRSCHEDEVERVPHNPYATLRLSRKAAAKSEHITKNIHKALVAEHLRGWYQRASGQKEQGHSTQTSFDSDRGSQRSLGFAGLQVPCSPSSRASSYSSVSSTNASGNWRTQITIGLSEYETPAHSSYTSCYGNIYNPLPSPGRPYTETGQLDNTDGNRLEDSLENSEQRLFWHEDSKPGTLV

Target ORF information:

RefSeq Version	XM_007192432.1
Organism	Balaenoptera acutorostrata scammoni
Definition	Balaenoptera acutorostrata scammoni FERM domain containing 4B (FRMD4B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007192432.1

ORF Insert Sequence:

ATGAGCCGAG ACCATGGTTC GGAGATGACA GAAGGCAGGC ACTGCCAGGT GCACCTCCTG 
GATGACAGGA GATTGGAGCT GCTGGTTCAG CCCAAACTTT TATCAAGAGA GTTGCTGGAC 
CTCGTGGCTT CGCATTTCAA CCTGAAAGAA AAGGAGTATT TTGGAATCAC GTTTACAGAT 
GACACAGGTC AGCAGAACTG GCTACAGTTA GACCACCGAG TTCTTGACCA CGATTTGCCC 
AAGAAACCAG GCCCAACCGT TTTGCACTTT TCTGTGAGGT TTTACATTGA GAGCATATCG 
TTCCTGAAGG ATAAAACCAC CGTGGAGCTG TTTTTCCTGA ATGCAAAGGC CTGTGTGCAC 
AAGGGGCAAA TTGAAGTAGA CAGCGAAACT ATCTTCAGGT TAGCGGCGCT TATTTTACAG 
GAAGCCAAAG GGGATTACAC CAGTGATGAA AATGCCAGGA AAGATTTAAA GACATTACCA 
GCCTTTCCAA CCAAAACTCT GCAGGAGCAC CCCTCCCTTG CCTACTGTGA GGACAGGGTA 
ATTGAGCATT ATTTGAAAAT CAAAGGTCTG ACTCGGGGTC AAGCTGTGGT TCAGTATATG 
AAAATAGTAG AAGCTCTACC GACCTATGGA GTCCATTATT ATGCAGTAAA GGATAAACAA 
GGACTTCCTT GGTGGCTTGG AATCAGCTAT AAAGGAATTG GCCAGTATGA TTTGCAAGAT 
AAGGTGAAAC CTCGGAAATT ATTCCAGTGG AAACAGCTGG AGAACTTATA TTTCCGTGAG 
AAAAAGTTTG CTGTGGAAGT TCACGATCCA CGGAGGATTT CGGTATCCAG AAGAACCTTT 
GGGCAAAGTG GCCTCTTTGT GCAAACGTGG TATGCGAACT CTTCTCTCAT CAAGTCCATC 
TGGGTAATGG CAATTAGTCA GCATCAGTTT TACTTGGACC GGAAGCAAAG CAAAGCAAAA 
ATTCCTTCAG CCAGGAGTTT AGACGATATT GCAATGGATT TGACTGAGAC AGGAACACCA 
AGAGTCTCCA AAATGGTGAC ACTAGAGGCA AAAAGTCAGT TCATCATGGC AAGCAACGGC 
AGTTTAATCT CCTCAGGTTC TCAAGACTCG GAAGTCAGTG AGGAGCAAAA GAGAGAGAAA 
ATCCTTGAAC TCAAGAAGAA GGAAAAGCTG TTGCAAGAAA AACTTCTGAA AAAAGTTGAA 
GAACTTAAGA AGATCTGTTT GCGGGAGGCT GAGCTCACAG GCAAAATGCC AAAGGAGTAT 
CCACTGAACG TAGGTGAGAA AGCTCCTCAC GTCAGAAGGC GTGTAGGTAC CGCATTCAAA 
TTAGATGACA ACTTGCTGCC GAGTGAAGAG GACCCTGCTT TGCAAGAACT GGAGAGCAAT 
TTCCTAATAC AGCAAAAGCT GGTTGAAGCT GCAAAGAAAC TTGCCAACGA GCCAGATCTT 
TGTAAAACTG TGAAGAAAAA ACGAAAGCAA GATTACACGG ATGCAGTGAA AAAGCTTCAG 
GAGATTGAAA ATGCAATAAA TGAATACCGA ATTAGATGTG GAAAGAAACC CAGCCAGAAA 
GCAACAGTTA TCGTACCAGA AGACATAATC CCATCAGAAA GTAGCTCCTT GTCTGACACT 
ACCACCTATG ATGACCCCAG TGACACCTGT ACTCTTGCTG GGCAGCAATC AAGTTCAGTA 
CCTCACTCTC CAAGAATTCT TCCTCCCAAG TCTCTCGGGA TTGAGAGAAT CCATTTCAGA 
AAGTCGTCCA TCAATGAACA GTTTGTGGAT ACCAGACAGT GCAGAGAAAT GCTGTCGACA 
CACAGCAGTC CTTACAAAAC TCTGGAGAGG CGGCCCCAGG GAGGGCGAAG CGTGCCCACC 
ACACCTGTTC TCACCCGGAA CGCCTACAGC AGCAGCCACT TGCAACCCGA ATCTTCATCT 
CAGCACTGCC GCCAGCGGAG TGGAAGCCTT GAGTCCCAGT CTCATCTGCT CTCTGAGATG 
GACCACGATA AGCCATTTCC CTCCCTCTCC AAATCCCAAA GAAGCAGCAG CACAGAAATC 
CTGGATGACG GGTCTTCCTA TACCAGCCAG TCCAGCACAG AGTATTACTG CGTGACACCC 
GTGGCCGGCC CCTGTTACAC CACCCAAACT CTGGACACTC GCTCCAGGGG TCGGAGAAGG 
TCCAAGAAAC AGAACGTTTC GACTTCAAAT TCAGGAAGCA TGCCCAACCT AGCACAAAAG 
GATAGTGTGA GGAATGGCGT CTACTCAAAG AGTCAGGAGC AGCCTTCTTC CAATTACTAC 
ACTGCCGGGT ACACGCCACA TGCAGAGTGT GATCTCTATT ACAGCGGTGG CTACGTGTAC 
GAGAACGACA CTGAAGGACA GTACAGCGTC AATCCTTCCT ACCGGTCCTC CGCCCACTAT 
GGATATGACC ACCCAAGGGA CTACAGCAGG TCGTGTCATG AAGACGAGGT CGAGCGGGTG 
CCCCATAACC CGTATGCAAC TCTCCGGCTG TCGAGGAAGG CTGCTGCCAA GTCAGAGCAC 
ATCACCAAGA ACATCCACAA GGCCTTAGTG GCCGAGCACC TGCGTGGCTG GTACCAGCGG 
GCCTCTGGGC AAAAGGAGCA GGGCCACAGC ACACAGACTA GCTTTGATTC GGACAGGGGA 
TCGCAGAGAA GCCTGGGCTT TGCCGGGCTG CAGGTACCCT GTTCTCCAAG CAGCCGAGCG 
TCTTCCTACT CTTCAGTGTC TTCTACAAAT GCTTCCGGGA ACTGGAGGAC CCAGATAACT 
ATAGGGCTAT CTGAATACGA GACCCCGGCA CATTCTTCCT ACACCAGCTG CTATGGCAAC 
ATCTATAATC CTTTGCCCTC TCCAGGCAGA CCATACACAG AGACCGGTCA ACTGGACAAT 
ACAGATGGAA ACCGGCTGGA AGACAGCCTG GAGAATAGTG AGCAGAGGCT CTTTTGGCAT 
GAAGACTCAA AGCCTGGAAC ATTAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FRMD4B ( XM_007192431.1 ) cDNA ORF clone, Balaenoptera acutorostrata scammoni -> XP_007192493.1 Balaenoptera acutorostrata scammoni FERM domain containing 4B (FRMD4B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa170515
Clone ID Related Accession (Same CDS sequence)	XM_007192431.1
Accession Version	XM_007192431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-11
Organism	Balaenoptera acutorostrata scammoni
Product	FERM domain-containing protein 4B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006731900.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Balaenoptera acutorostrata scammoni Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG TGTTCATGTG CGGAGTGGAG GACCTGCTGT TCAGCGGCAG CCGCTTCGTG 
TGGAACTTGA CCGTGTCCAC GCTGCGGAGA TGGTACACAG AGAGGCTGCG GGCTTGCCAC 
CAGGTGCTGC GGACGTGGTG CGGACTGCGG GACGTGTACC AGATGACAGA AGGCAGGCAC 
TGCCAGGTGC ACCTCCTGGA TGACAGGAGA TTGGAGCTGC TGGTTCAGCC CAAACTTTTA 
TCAAGAGAGT TGCTGGACCT CGTGGCTTCG CATTTCAACC TGAAAGAAAA GGAGTATTTT 
GGAATCACGT TTACAGATGA CACAGGTCAG CAGAACTGGC TACAGTTAGA CCACCGAGTT 
CTTGACCACG ATTTGCCCAA GAAACCAGGC CCAACCGTTT TGCACTTTTC TGTGAGGTTT 
TACATTGAGA GCATATCGTT CCTGAAGGAT AAAACCACCG TGGAGCTGTT TTTCCTGAAT 
GCAAAGGCCT GTGTGCACAA GGGGCAAATT GAAGTAGACA GCGAAACTAT CTTCAGGTTA 
GCGGCGCTTA TTTTACAGGA AGCCAAAGGG GATTACACCA GTGATGAAAA TGCCAGGAAA 
GATTTAAAGA CATTACCAGC CTTTCCAACC AAAACTCTGC AGGAGCACCC CTCCCTTGCC 
TACTGTGAGG ACAGGGTAAT TGAGCATTAT TTGAAAATCA AAGGTCTGAC TCGGGGTCAA 
GCTGTGGTTC AGTATATGAA AATAGTAGAA GCTCTACCGA CCTATGGAGT CCATTATTAT 
GCAGTAAAGG ATAAACAAGG ACTTCCTTGG TGGCTTGGAA TCAGCTATAA AGGAATTGGC 
CAGTATGATT TGCAAGATAA GGTGAAACCT CGGAAATTAT TCCAGTGGAA ACAGCTGGAG 
AACTTATATT TCCGTGAGAA AAAGTTTGCT GTGGAAGTTC ACGATCCACG GAGGATTTCG 
GTATCCAGAA GAACCTTTGG GCAAAGTGGC CTCTTTGTGC AAACGTGGTA TGCGAACTCT 
TCTCTCATCA AGTCCATCTG GGTAATGGCA ATTAGTCAGC ATCAGTTTTA CTTGGACCGG 
AAGCAAAGCA AAGCAAAAAT TCCTTCAGCC AGGAGTTTAG ACGATATTGC AATGGATTTG 
ACTGAGACAG GAACACCAAG AGTCTCCAAA ATGGTGACAC TAGAGGCAAA AAGTCAGTTC 
ATCATGGCAA GCAACGGCAG TTTAATCTCC TCAGGTTCTC AAGACTCGGA AGTCAGTGAG 
GAGCAAAAGA GAGAGAAAAT CCTTGAACTC AAGAAGAAGG AAAAGCTGTT GCAAGAAAAA 
CTTCTGAAAA AAGTTGAAGA ACTTAAGAAG ATCTGTTTGC GGGAGGCTGA GCTCACAGGC 
AAAATGCCAA AGGAGTATCC ACTGAACGTA GGTGAGAAAG CTCCTCACGT CAGAAGGCGT 
GTAGGTACCG CATTCAAATT AGATGACAAC TTGCTGCCGA GTGAAGAGGA CCCTGCTTTG 
CAAGAACTGG AGAGCAATTT CCTAATACAG CAAAAGCTGG TTGAAGCTGC AAAGAAACTT 
GCCAACGAGC CAGATCTTTG TAAAACTGTG AAGAAAAAAC GAAAGCAAGA TTACACGGAT 
GCAGTGAAAA AGCTTCAGGA GATTGAAAAT GCAATAAATG AATACCGAAT TAGATGTGGA 
AAGAAACCCA GCCAGAAAGC AACAGTTATC GTACCAGAAG ACATAATCCC ATCAGAAAGT 
AGCTCCTTGT CTGACACTAC CACCTATGAT GACCCCAGTG ACACCTGTAC TCTTGCTGGG 
CAGCAATCAA GTTCAGTACC TCACTCTCCA AGAATTCTTC CTCCCAAGTC TCTCGGGATT 
GAGAGAATCC ATTTCAGAAA GTCGTCCATC AATGAACAGT TTGTGGATAC CAGACAGTGC 
AGAGAAATGC TGTCGACACA CAGCAGTCCT TACAAAACTC TGGAGAGGCG GCCCCAGGGA 
GGGCGAAGCG TGCCCACCAC ACCTGTTCTC ACCCGGAACG CCTACAGCAG CAGCCACTTG 
CAACCCGAAT CTTCATCTCA GCACTGCCGC CAGCGGAGTG GAAGCCTTGA GTCCCAGTCT 
CATCTGCTCT CTGAGATGGA CCACGATAAG CCATTTCCCT CCCTCTCCAA ATCCCAAAGA 
AGCAGCAGCA CAGAAATCCT GGATGACGGG TCTTCCTATA CCAGCCAGTC CAGCACAGAG 
TATTACTGCG TGACACCCGT GGCCGGCCCC TGTTACACCA CCCAAACTCT GGACACTCGC 
TCCAGGGGTC GGAGAAGGTC CAAGAAACAG AACGTTTCGA CTTCAAATTC AGGAAGCATG 
CCCAACCTAG CACAAAAGGA TAGTGTGAGG AATGGCGTCT ACTCAAAGAG TCAGGAGCAG 
CCTTCTTCCA ATTACTACAC TGCCGGGTAC ACGCCACATG CAGAGTGTGA TCTCTATTAC 
AGCGGTGGCT ACGTGTACGA GAACGACACT GAAGGACAGT ACAGCGTCAA TCCTTCCTAC 
CGGTCCTCCG CCCACTATGG ATATGACCAC CCAAGGGACT ACAGCAGGTC GTGTCATGAA 
GACGAGGTCG AGCGGGTGCC CCATAACCCG TATGCAACTC TCCGGCTGTC GAGGAAGGCT 
GCTGCCAAGT CAGAGCACAT CACCAAGAAC ATCCACAAGG CCTTAGTGGC CGAGCACCTG 
CGTGGCTGGT ACCAGCGGGC CTCTGGGCAA AAGGAGCAGG GCCACAGCAC ACAGACTAGC 
TTTGATTCGG ACAGGGGATC GCAGAGAAGC CTGGGCTTTG CCGGGCTGCA GGTACCCTGT 
TCTCCAAGCA GCCGAGCGTC TTCCTACTCT TCAGTGTCTT CTACAAATGC TTCCGGGAAC 
TGGAGGACCC AGATAACTAT AGGGCTATCT GAATACGAGA CCCCGGCACA TTCTTCCTAC 
ACCAGCTGCT ATGGCAACAT CTATAATCCT TTGCCCTCTC CAGGCAGACC ATACACAGAG 
ACCGGTCAAC TGGACAATAC AGATGGAAAC CGGCTGGAAG ACAGCCTGGA GAATAGTGAG 
CAGAGGCTCT TTTGGCATGA AGACTCAAAG CCTGGAACAT TAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007192493.1
CDS	60..3167
Translation	MASVFMCGVEDLLFSGSRFVWNLTVSTLRRWYTERLRACHQVLRTWCGLRDVYQMTEGRHCQVHLLDDRRLELLVQPKLLSRELLDLVASHFNLKEKEYFGITFTDDTGQQNWLQLDHRVLDHDLPKKPGPTVLHFSVRFYIESISFLKDKTTVELFFLNAKACVHKGQIEVDSETIFRLAALILQEAKGDYTSDENARKDLKTLPAFPTKTLQEHPSLAYCEDRVIEHYLKIKGLTRGQAVVQYMKIVEALPTYGVHYYAVKDKQGLPWWLGISYKGIGQYDLQDKVKPRKLFQWKQLENLYFREKKFAVEVHDPRRISVSRRTFGQSGLFVQTWYANSSLIKSIWVMAISQHQFYLDRKQSKAKIPSARSLDDIAMDLTETGTPRVSKMVTLEAKSQFIMASNGSLISSGSQDSEVSEEQKREKILELKKKEKLLQEKLLKKVEELKKICLREAELTGKMPKEYPLNVGEKAPHVRRRVGTAFKLDDNLLPSEEDPALQELESNFLIQQKLVEAAKKLANEPDLCKTVKKKRKQDYTDAVKKLQEIENAINEYRIRCGKKPSQKATVIVPEDIIPSESSSLSDTTTYDDPSDTCTLAGQQSSSVPHSPRILPPKSLGIERIHFRKSSINEQFVDTRQCREMLSTHSSPYKTLERRPQGGRSVPTTPVLTRNAYSSSHLQPESSSQHCRQRSGSLESQSHLLSEMDHDKPFPSLSKSQRSSSTEILDDGSSYTSQSSTEYYCVTPVAGPCYTTQTLDTRSRGRRRSKKQNVSTSNSGSMPNLAQKDSVRNGVYSKSQEQPSSNYYTAGYTPHAECDLYYSGGYVYENDTEGQYSVNPSYRSSAHYGYDHPRDYSRSCHEDEVERVPHNPYATLRLSRKAAAKSEHITKNIHKALVAEHLRGWYQRASGQKEQGHSTQTSFDSDRGSQRSLGFAGLQVPCSPSSRASSYSSVSSTNASGNWRTQITIGLSEYETPAHSSYTSCYGNIYNPLPSPGRPYTETGQLDNTDGNRLEDSLENSEQRLFWHEDSKPGTLV

Target ORF information:

RefSeq Version	XM_007192431.1
Organism	Balaenoptera acutorostrata scammoni
Definition	Balaenoptera acutorostrata scammoni FERM domain containing 4B (FRMD4B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007192431.1

ORF Insert Sequence:

ATGGCCTCGG TGTTCATGTG CGGAGTGGAG GACCTGCTGT TCAGCGGCAG CCGCTTCGTG 
TGGAACTTGA CCGTGTCCAC GCTGCGGAGA TGGTACACAG AGAGGCTGCG GGCTTGCCAC 
CAGGTGCTGC GGACGTGGTG CGGACTGCGG GACGTGTACC AGATGACAGA AGGCAGGCAC 
TGCCAGGTGC ACCTCCTGGA TGACAGGAGA TTGGAGCTGC TGGTTCAGCC CAAACTTTTA 
TCAAGAGAGT TGCTGGACCT CGTGGCTTCG CATTTCAACC TGAAAGAAAA GGAGTATTTT 
GGAATCACGT TTACAGATGA CACAGGTCAG CAGAACTGGC TACAGTTAGA CCACCGAGTT 
CTTGACCACG ATTTGCCCAA GAAACCAGGC CCAACCGTTT TGCACTTTTC TGTGAGGTTT 
TACATTGAGA GCATATCGTT CCTGAAGGAT AAAACCACCG TGGAGCTGTT TTTCCTGAAT 
GCAAAGGCCT GTGTGCACAA GGGGCAAATT GAAGTAGACA GCGAAACTAT CTTCAGGTTA 
GCGGCGCTTA TTTTACAGGA AGCCAAAGGG GATTACACCA GTGATGAAAA TGCCAGGAAA 
GATTTAAAGA CATTACCAGC CTTTCCAACC AAAACTCTGC AGGAGCACCC CTCCCTTGCC 
TACTGTGAGG ACAGGGTAAT TGAGCATTAT TTGAAAATCA AAGGTCTGAC TCGGGGTCAA 
GCTGTGGTTC AGTATATGAA AATAGTAGAA GCTCTACCGA CCTATGGAGT CCATTATTAT 
GCAGTAAAGG ATAAACAAGG ACTTCCTTGG TGGCTTGGAA TCAGCTATAA AGGAATTGGC 
CAGTATGATT TGCAAGATAA GGTGAAACCT CGGAAATTAT TCCAGTGGAA ACAGCTGGAG 
AACTTATATT TCCGTGAGAA AAAGTTTGCT GTGGAAGTTC ACGATCCACG GAGGATTTCG 
GTATCCAGAA GAACCTTTGG GCAAAGTGGC CTCTTTGTGC AAACGTGGTA TGCGAACTCT 
TCTCTCATCA AGTCCATCTG GGTAATGGCA ATTAGTCAGC ATCAGTTTTA CTTGGACCGG 
AAGCAAAGCA AAGCAAAAAT TCCTTCAGCC AGGAGTTTAG ACGATATTGC AATGGATTTG 
ACTGAGACAG GAACACCAAG AGTCTCCAAA ATGGTGACAC TAGAGGCAAA AAGTCAGTTC 
ATCATGGCAA GCAACGGCAG TTTAATCTCC TCAGGTTCTC AAGACTCGGA AGTCAGTGAG 
GAGCAAAAGA GAGAGAAAAT CCTTGAACTC AAGAAGAAGG AAAAGCTGTT GCAAGAAAAA 
CTTCTGAAAA AAGTTGAAGA ACTTAAGAAG ATCTGTTTGC GGGAGGCTGA GCTCACAGGC 
AAAATGCCAA AGGAGTATCC ACTGAACGTA GGTGAGAAAG CTCCTCACGT CAGAAGGCGT 
GTAGGTACCG CATTCAAATT AGATGACAAC TTGCTGCCGA GTGAAGAGGA CCCTGCTTTG 
CAAGAACTGG AGAGCAATTT CCTAATACAG CAAAAGCTGG TTGAAGCTGC AAAGAAACTT 
GCCAACGAGC CAGATCTTTG TAAAACTGTG AAGAAAAAAC GAAAGCAAGA TTACACGGAT 
GCAGTGAAAA AGCTTCAGGA GATTGAAAAT GCAATAAATG AATACCGAAT TAGATGTGGA 
AAGAAACCCA GCCAGAAAGC AACAGTTATC GTACCAGAAG ACATAATCCC ATCAGAAAGT 
AGCTCCTTGT CTGACACTAC CACCTATGAT GACCCCAGTG ACACCTGTAC TCTTGCTGGG 
CAGCAATCAA GTTCAGTACC TCACTCTCCA AGAATTCTTC CTCCCAAGTC TCTCGGGATT 
GAGAGAATCC ATTTCAGAAA GTCGTCCATC AATGAACAGT TTGTGGATAC CAGACAGTGC 
AGAGAAATGC TGTCGACACA CAGCAGTCCT TACAAAACTC TGGAGAGGCG GCCCCAGGGA 
GGGCGAAGCG TGCCCACCAC ACCTGTTCTC ACCCGGAACG CCTACAGCAG CAGCCACTTG 
CAACCCGAAT CTTCATCTCA GCACTGCCGC CAGCGGAGTG GAAGCCTTGA GTCCCAGTCT 
CATCTGCTCT CTGAGATGGA CCACGATAAG CCATTTCCCT CCCTCTCCAA ATCCCAAAGA 
AGCAGCAGCA CAGAAATCCT GGATGACGGG TCTTCCTATA CCAGCCAGTC CAGCACAGAG 
TATTACTGCG TGACACCCGT GGCCGGCCCC TGTTACACCA CCCAAACTCT GGACACTCGC 
TCCAGGGGTC GGAGAAGGTC CAAGAAACAG AACGTTTCGA CTTCAAATTC AGGAAGCATG 
CCCAACCTAG CACAAAAGGA TAGTGTGAGG AATGGCGTCT ACTCAAAGAG TCAGGAGCAG 
CCTTCTTCCA ATTACTACAC TGCCGGGTAC ACGCCACATG CAGAGTGTGA TCTCTATTAC 
AGCGGTGGCT ACGTGTACGA GAACGACACT GAAGGACAGT ACAGCGTCAA TCCTTCCTAC 
CGGTCCTCCG CCCACTATGG ATATGACCAC CCAAGGGACT ACAGCAGGTC GTGTCATGAA 
GACGAGGTCG AGCGGGTGCC CCATAACCCG TATGCAACTC TCCGGCTGTC GAGGAAGGCT 
GCTGCCAAGT CAGAGCACAT CACCAAGAAC ATCCACAAGG CCTTAGTGGC CGAGCACCTG 
CGTGGCTGGT ACCAGCGGGC CTCTGGGCAA AAGGAGCAGG GCCACAGCAC ACAGACTAGC 
TTTGATTCGG ACAGGGGATC GCAGAGAAGC CTGGGCTTTG CCGGGCTGCA GGTACCCTGT 
TCTCCAAGCA GCCGAGCGTC TTCCTACTCT TCAGTGTCTT CTACAAATGC TTCCGGGAAC 
TGGAGGACCC AGATAACTAT AGGGCTATCT GAATACGAGA CCCCGGCACA TTCTTCCTAC 
ACCAGCTGCT ATGGCAACAT CTATAATCCT TTGCCCTCTC CAGGCAGACC ATACACAGAG 
ACCGGTCAAC TGGACAATAC AGATGGAAAC CGGCTGGAAG ACAGCCTGGA GAATAGTGAG 
CAGAGGCTCT TTTGGCATGA AGACTCAAAG CCTGGAACAT TAGTCTGA