BO88DRAFT_389539 cDNA ORF clone,

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gRNAs

The following BO88DRAFT_389539 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BO88DRAFT_389539 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs348321	XM_025704917.1 Latest version!	Aspergillus vadensis CBS 113365 histidine-tagged glucoamylase (BO88DRAFT_389539), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BO88DRAFT_389539 ( XM_025704917.1 ) cDNA ORF clone, -> XP_025562230.1 Aspergillus vadensis CBS 113365 histidine-tagged glucoamylase (BO88DRAFT_389539), mRNA.

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CloneID	OAs348321
Clone ID Related Accession (Same CDS sequence)	XM_025704917.1
Accession Version	XM_025704917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-10
Organism	Aspergillus vadensis CBS 113365
Product	histidine-tagged glucoamylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_020292051). ##Metadata-START## Organism Display Name :: Aspergillus vadensis CBS 113365 v1.0 GOLD Stamp ID :: Gp0047311 ##Metadata-END##

ATGTCGTTCC GATCTCTTCT TGCCCTGAGC GGCCTCGTCT GCTCGGGGTT GGCAAGTGTT 
ATTTCTAAGC GCGCGACCTT GGATTCGTGG TTGAGCAACG AAGCGACCGT GGCTCGTACT 
GCGATCCTGA ATAACATCGG GGCGGACGGT GCTTGGGTGT CGGGCGCGGA CTCGGGCATT 
GTCGTTGCCA GTCCCAGCAC CGATAACCCG GACTACTTCT ACACTTGGAC TCGCGACTCG 
GGTCTCGTCA TCAAGACCCT CGTCGATCTC TTCCGCAATG GAGATACTGA TCTCCTTTCC 
ACCATTGAGA ACTACATCTC CTCTCAGGCA ATTGTTCAGG GTATCAGTAA CCCCTCTGGT 
GATCTGTCCA GCGGCGGTCT TGGTGAGCCC AAGTTCAATG TCGATGAGAC TGCCTACACT 
GGGTCTTGGG GACGGCCGCA GCGTGATGGT CCTGCCCTGA GAGCAACTGC TATGATCGGC 
TTTGGGCAGT GGCTGCTTGA CAATGGCTAC ACCAGCGCTG CAACGGAGAT TGTTTGGCCC 
CTCGTTAGGA ACGACCTGTC GTATGTGGCT CAGTACTGGA ACCAGACGGG ATATGATCTC 
TGGGAAGAAG TTAATGGCTC GTCTTTCTTC ACTATTGCTG TGCAGCACCG CGCCCTCGTC 
GAAGGTAGTG CCTTCGCGAC GGCCGTCGGC TCGTCCTGCT CCTGGTGTGA TTCGCAGGCA 
CCTCAGATTC TCTGCTACCT GCAGTCCTTC TGGACCGGCG ACTACATCCT GGCCAACTTT 
GATAGCAGCC GTTCCGGCAA GGACACCAAC ACCCTCCTGG GAAGCATCCA CACCTTTGAT 
CCTGAGGCTG GATGCGATGA TTCCACCTTC CAGCCCTGCT CCCCGCGTGC GCTTGCCAAC 
CACAAGGAGG TTGTAGACTC TTTCCGCTCG ATTTATACTC TCAACGATGG TCTCAGTGAC 
AGTGAGGCGG TTGCAGTCGG TCGGTACCCC GAGGATACGT ACTACAACGG CAACCCGTGG 
TTCCTGTGCA CCTTGGCTGC CGCGGAGCAG CTGTACGATG CTCTGTACCA GTGGGACAAG 
CAGGGGTCCT TGGAGATCAC AGACGTGTCA CTTGACTTCT TCCAGGCTCT GTACAGTGAT 
GCTGCCACCG GCACGTACTC ATCGTCCAGC TCGACCTATA GCAGCATTGT GGATGCCGTG 
AAGACTTTCG CTGATGGTTT CGTTTCTATT GTGGAAACTC ACGCCGCAAG CAACGGCTCT 
CTTTCCGAGC AATACGACAA GTCTGATGGC GACGAGCTTT CTGCTCGCGA CCTGACCTGG 
TCTTACGCTG CTCTGCTGAC CGCCAACAAC CGTCGTAACT CTGTTGTGCC CCCATCCTGG 
GGCGAGACCT CTGCCAGCAG CGTGCCCGGC ACCTGTGCGG CTACCTCTGC CTCTGGTACC 
TACAGCAGTG TGACCGTCAC CTCGTGGCCG AGCATTGTGG CTACTGGTGG CACCACTACG 
ACGGCTACCA CCACTGGATC CGGCGGCGTG ACCTCGACCA GCAAGGCCAC CACAACTGCT 
AGTAAGACCA GCACCACTAC GTCCTCGACC TCCTGCACCA CTCCCACCGC CGTAGCTGTG 
ACCTTTGATC TGACGGCCAC CACCACCTAC GGCGAGAACA TCTACCTGGT CGGGTCGATC 
TCTCAGCTCG GTGACTGGGA CACCAGCGAC GGCATAGCTC TGAGCGCTGA CAAGTACACT 
TCCAGCAACC CGCTCTGGTA TGTAACTGTG ACTCTGCCGG CTGGTGAGTC GTTTGAGTAC 
AAATTCATTC GCATTGAGAG CGATGACTCC GTGGAGTGGG AGAGTGACCC GAACCGGGAA 
TACACGGTTC CTCAGGCGTG CGGAGAGTCG ACCGCGACGG TGACTGACAC CTGGCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025562230.1
CDS	264..2183
Translation	MSFRSLLALSGLVCSGLASVISKRATLDSWLSNEATVARTAILNNIGADGAWVSGADSGIVVASPSTDNPDYFYTWTRDSGLVIKTLVDLFRNGDTDLLSTIENYISSQAIVQGISNPSGDLSSGGLGEPKFNVDETAYTGSWGRPQRDGPALRATAMIGFGQWLLDNGYTSAATEIVWPLVRNDLSYVAQYWNQTGYDLWEEVNGSSFFTIAVQHRALVEGSAFATAVGSSCSWCDSQAPQILCYLQSFWTGDYILANFDSSRSGKDTNTLLGSIHTFDPEAGCDDSTFQPCSPRALANHKEVVDSFRSIYTLNDGLSDSEAVAVGRYPEDTYYNGNPWFLCTLAAAEQLYDALYQWDKQGSLEITDVSLDFFQALYSDAATGTYSSSSSTYSSIVDAVKTFADGFVSIVETHAASNGSLSEQYDKSDGDELSARDLTWSYAALLTANNRRNSVVPPSWGETSASSVPGTCAATSASGTYSSVTVTSWPSIVATGGTTTTATTTGSGGVTSTSKATTTASKTSTTTSSTSCTTPTAVAVTFDLTATTTYGENIYLVGSISQLGDWDTSDGIALSADKYTSSNPLWYVTVTLPAGESFEYKFIRIESDDSVEWESDPNREYTVPQACGESTATVTDTWR

Target ORF information:

RefSeq Version	XM_025704917.1
Organism	Aspergillus vadensis CBS 113365
Definition	Aspergillus vadensis CBS 113365 histidine-tagged glucoamylase (BO88DRAFT_389539), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025704917.1

ORF Insert Sequence:

ATGTCGTTCC GATCTCTTCT TGCCCTGAGC GGCCTCGTCT GCTCGGGGTT GGCAAGTGTT 
ATTTCTAAGC GCGCGACCTT GGATTCGTGG TTGAGCAACG AAGCGACCGT GGCTCGTACT 
GCGATCCTGA ATAACATCGG GGCGGACGGT GCTTGGGTGT CGGGCGCGGA CTCGGGCATT 
GTCGTTGCCA GTCCCAGCAC CGATAACCCG GACTACTTCT ACACTTGGAC TCGCGACTCG 
GGTCTCGTCA TCAAGACCCT CGTCGATCTC TTCCGCAATG GAGATACTGA TCTCCTTTCC 
ACCATTGAGA ACTACATCTC CTCTCAGGCA ATTGTTCAGG GTATCAGTAA CCCCTCTGGT 
GATCTGTCCA GCGGCGGTCT TGGTGAGCCC AAGTTCAATG TCGATGAGAC TGCCTACACT 
GGGTCTTGGG GACGGCCGCA GCGTGATGGT CCTGCCCTGA GAGCAACTGC TATGATCGGC 
TTTGGGCAGT GGCTGCTTGA CAATGGCTAC ACCAGCGCTG CAACGGAGAT TGTTTGGCCC 
CTCGTTAGGA ACGACCTGTC GTATGTGGCT CAGTACTGGA ACCAGACGGG ATATGATCTC 
TGGGAAGAAG TTAATGGCTC GTCTTTCTTC ACTATTGCTG TGCAGCACCG CGCCCTCGTC 
GAAGGTAGTG CCTTCGCGAC GGCCGTCGGC TCGTCCTGCT CCTGGTGTGA TTCGCAGGCA 
CCTCAGATTC TCTGCTACCT GCAGTCCTTC TGGACCGGCG ACTACATCCT GGCCAACTTT 
GATAGCAGCC GTTCCGGCAA GGACACCAAC ACCCTCCTGG GAAGCATCCA CACCTTTGAT 
CCTGAGGCTG GATGCGATGA TTCCACCTTC CAGCCCTGCT CCCCGCGTGC GCTTGCCAAC 
CACAAGGAGG TTGTAGACTC TTTCCGCTCG ATTTATACTC TCAACGATGG TCTCAGTGAC 
AGTGAGGCGG TTGCAGTCGG TCGGTACCCC GAGGATACGT ACTACAACGG CAACCCGTGG 
TTCCTGTGCA CCTTGGCTGC CGCGGAGCAG CTGTACGATG CTCTGTACCA GTGGGACAAG 
CAGGGGTCCT TGGAGATCAC AGACGTGTCA CTTGACTTCT TCCAGGCTCT GTACAGTGAT 
GCTGCCACCG GCACGTACTC ATCGTCCAGC TCGACCTATA GCAGCATTGT GGATGCCGTG 
AAGACTTTCG CTGATGGTTT CGTTTCTATT GTGGAAACTC ACGCCGCAAG CAACGGCTCT 
CTTTCCGAGC AATACGACAA GTCTGATGGC GACGAGCTTT CTGCTCGCGA CCTGACCTGG 
TCTTACGCTG CTCTGCTGAC CGCCAACAAC CGTCGTAACT CTGTTGTGCC CCCATCCTGG 
GGCGAGACCT CTGCCAGCAG CGTGCCCGGC ACCTGTGCGG CTACCTCTGC CTCTGGTACC 
TACAGCAGTG TGACCGTCAC CTCGTGGCCG AGCATTGTGG CTACTGGTGG CACCACTACG 
ACGGCTACCA CCACTGGATC CGGCGGCGTG ACCTCGACCA GCAAGGCCAC CACAACTGCT 
AGTAAGACCA GCACCACTAC GTCCTCGACC TCCTGCACCA CTCCCACCGC CGTAGCTGTG 
ACCTTTGATC TGACGGCCAC CACCACCTAC GGCGAGAACA TCTACCTGGT CGGGTCGATC 
TCTCAGCTCG GTGACTGGGA CACCAGCGAC GGCATAGCTC TGAGCGCTGA CAAGTACACT 
TCCAGCAACC CGCTCTGGTA TGTAACTGTG ACTCTGCCGG CTGGTGAGTC GTTTGAGTAC 
AAATTCATTC GCATTGAGAG CGATGACTCC GTGGAGTGGG AGAGTGACCC GAACCGGGAA 
TACACGGTTC CTCAGGCGTG CGGAGAGTCG ACCGCGACGG TGACTGACAC CTGGCGGTAG